e faecium atcc 19433  (ATCC)


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  • 98
    Name:
    Enterococcus faecalis NCTC 775 DSM 20478 JCM 8726 NCDO 581 Tissier
    Description:

    Catalog Number:
    19433
    Price:
    None
    Applications:
    Control CultureMedia testingQuality control strainReference materialControl strain for Group DQuality control strain for Difco productsWater testing
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    Structured Review

    ATCC e faecium atcc 19433

    https://www.bioz.com/result/e faecium atcc 19433/product/ATCC
    Average 98 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e faecium atcc 19433 - by Bioz Stars, 2020-08
    98/100 stars

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    Positive Control:

    Article Title: Antibiotic Susceptibilities of Enterococcus Species Isolated from Hospital and Domestic Wastewater Effluents in Alice, Eastern Cape Province of South Africa
    Article Snippet: .. The positive control used was E. feacalis ATCC 19433. ..

    Incubation:

    Article Title: Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen
    Article Snippet: .. The root canals were inoculated with a fresh suspension of E. faecalis (ATCC 19433) at 109 CFU/mL in BHI, followed by incubation at 37°C, according to Soares et al. [ ]. ..

    other:

    Article Title: Most Probable Number - Loop Mediated Isothermal Amplification (MPN-LAMP) for Quantifying Waterborne Pathogens in Less Than 25 Minutes
    Article Snippet: Enterococcus faecalis was obtained from American Type Culture Collection (ATCC, 19433, Manassas, VA).

    Activity Assay:

    Article Title: Anti-biofilm effects and characterisation of the hydrogen peroxide activity of a range of Western Australian honeys compared to Manuka and multifloral honeys
    Article Snippet: .. Antibacterial activity testing Reference isolates were obtained from the School of Biomedical Sciences at The University of Western Australia, and from PathWest Laboratory Medicine WA, and were as follows; Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 700699, Enterococcus faecalis NCTC 775, Pseudomonas aeruginosa ATCC BAA-47, P. aeruginosa ATCC 27853, Escherichia coli NCTC 10538 and E. coli ATCC 25922. ..

    Sequencing:

    Article Title: Mechanisms for Photoinactivation of Enterococcus faecalis in Seawater
    Article Snippet: .. Primers for sodA were designed using Primer Express software, version 2 (Applied Biosystems), based on the sodA sequence for E. faecalis (ATCC 19433) in GenBank, and primer concentrations were optimized. ..

    Western Blot:

    Article Title: Anti-biofilm effects and characterisation of the hydrogen peroxide activity of a range of Western Australian honeys compared to Manuka and multifloral honeys
    Article Snippet: .. Antibacterial activity testing Reference isolates were obtained from the School of Biomedical Sciences at The University of Western Australia, and from PathWest Laboratory Medicine WA, and were as follows; Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 700699, Enterococcus faecalis NCTC 775, Pseudomonas aeruginosa ATCC BAA-47, P. aeruginosa ATCC 27853, Escherichia coli NCTC 10538 and E. coli ATCC 25922. ..

    Software:

    Article Title: Mechanisms for Photoinactivation of Enterococcus faecalis in Seawater
    Article Snippet: .. Primers for sodA were designed using Primer Express software, version 2 (Applied Biosystems), based on the sodA sequence for E. faecalis (ATCC 19433) in GenBank, and primer concentrations were optimized. ..

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    ATCC e faecium
    Pulsed-field gel electrophoresis patterns of genomic DNA of Enterococcus <t>faecium</t> strains isolated from commercially available swine ( n = 9) and cattle ( n = 13) probiotics.
    E Faecium, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e faecium/product/ATCC
    Average 96 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    e faecium - by Bioz Stars, 2020-08
    96/100 stars
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    Pulsed-field gel electrophoresis patterns of genomic DNA of Enterococcus faecium strains isolated from commercially available swine ( n = 9) and cattle ( n = 13) probiotics.

    Journal: Journal of Animal Science

    Article Title: Antimicrobial resistance of Enterococcus faecium strains isolated from commercial probiotic products used in cattle and swine strains isolated from commercial probiotic products used in cattle and swine

    doi: 10.1093/jas/sky056

    Figure Lengend Snippet: Pulsed-field gel electrophoresis patterns of genomic DNA of Enterococcus faecium strains isolated from commercially available swine ( n = 9) and cattle ( n = 13) probiotics.

    Article Snippet: The DNA was isolated by suspending a single colony in nuclease-free water with 5% Chelex 100 resin (Bio-Rad Laboratories, Hercules, CA) and boiled for 10 min. An ATCC strain of E. faecium (ATCC 19434; American Type Culture Collection, Manassas, VA) was used as a positive control in the assay.

    Techniques: Pulsed-Field Gel, Electrophoresis, Isolation

    Binding of bacterial cells to immobilized Plg. Glu-Plg (20 μg/ml) and BSA (25 μg/ml) were immobilized onto diagnostic glass slides. Enterococcus faecium ATCC 19434, Lactobacillus crispatus ST1 and Staphylococcus aureus NCTC 8325-4 (5 × 10 8 cells/ml) were allowed to adhere to the immobilized proteins. Adhered bacteria were visualized by staining with Loeffler’s methylene blue. The number of adhered bacteria was quantified microscopically. The mean and the ± SD of adhered bacteria in 19 or 20 randomly chosen microscopic fields in a representative experiment are shown

    Journal: BMC Research Notes

    Article Title: Penicillin binding protein 3 of Staphylococcus aureus NCTC 8325-4 binds and activates human plasminogen

    doi: 10.1186/s13104-016-2190-4

    Figure Lengend Snippet: Binding of bacterial cells to immobilized Plg. Glu-Plg (20 μg/ml) and BSA (25 μg/ml) were immobilized onto diagnostic glass slides. Enterococcus faecium ATCC 19434, Lactobacillus crispatus ST1 and Staphylococcus aureus NCTC 8325-4 (5 × 10 8 cells/ml) were allowed to adhere to the immobilized proteins. Adhered bacteria were visualized by staining with Loeffler’s methylene blue. The number of adhered bacteria was quantified microscopically. The mean and the ± SD of adhered bacteria in 19 or 20 randomly chosen microscopic fields in a representative experiment are shown

    Article Snippet: E. faecium ATCC 19434 and S. aureus NCTC 8325-4 were grown shaking in 10 ml Todd-Hewitt broth (Difco; BD Biosciences) for 18 h at 37 °C.

    Techniques: Binding Assay, Diagnostic Assay, Staining

    PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Article Snippet: These referenced bacterial strains were as follow: Bacteroides fragilis ATCC 25285, VSE strains (E. faecalis NCTC 77, E faecium NCIMB 2699) and VRE strains (E faecium ATCC 19434, E faecalis ATCC 29212, E faecalis ATCC 51299).

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control

    Concentration dependence of RT-PCR-HRMA using the V1 primer set: Amplification curves for different quantities of DNA from VRE E faecalis ATCC 29212 (250 fg to 250 ng) using the V1 primer pair.

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: Concentration dependence of RT-PCR-HRMA using the V1 primer set: Amplification curves for different quantities of DNA from VRE E faecalis ATCC 29212 (250 fg to 250 ng) using the V1 primer pair.

    Article Snippet: These referenced bacterial strains were as follow: Bacteroides fragilis ATCC 25285, VSE strains (E. faecalis NCTC 77, E faecium NCIMB 2699) and VRE strains (E faecium ATCC 19434, E faecalis ATCC 29212, E faecalis ATCC 51299).

    Techniques: Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Amplification

    Analytical sensitivity of VRE PCR-HRMA assay using the V1 primer set. Data show that the characteristic difference curve shape for VRE (using DNA from VRE E faecalis strain ATCC 29212) is maintained over a 6-log concentration range (250 fg to 250 ng DNA).

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: Analytical sensitivity of VRE PCR-HRMA assay using the V1 primer set. Data show that the characteristic difference curve shape for VRE (using DNA from VRE E faecalis strain ATCC 29212) is maintained over a 6-log concentration range (250 fg to 250 ng DNA).

    Article Snippet: These referenced bacterial strains were as follow: Bacteroides fragilis ATCC 25285, VSE strains (E. faecalis NCTC 77, E faecium NCIMB 2699) and VRE strains (E faecium ATCC 19434, E faecalis ATCC 29212, E faecalis ATCC 51299).

    Techniques: Polymerase Chain Reaction, Concentration Assay

    PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Article Snippet: These referenced bacterial strains were as follow: Bacteroides fragilis ATCC 25285, VSE strains (E. faecalis NCTC 77, E faecium NCIMB 2699) and VRE strains (E faecium ATCC 19434, E faecalis ATCC 29212, E faecalis ATCC 51299).

    Techniques: Polymerase Chain Reaction, Generated

    PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains ( E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains ( E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Polymerase Chain Reaction, Generated

    PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains ( E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains ( E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control