Journal: Frontiers in Microbiology
Article Title: The Discovery of New Antilisterial Proteins From Paenibacillus polymyxa Kp10 via Genome Mining and Mass Spectrometry
doi: 10.3389/fmicb.2020.00960
Figure Lengend Snippet: In vitro antimicrobial activity assay of recombinant antilisterial proteins. Each crude recombinant protein was obtained from three different cultures and assayed in triplicates. (i) (A) P1_1, P1_2, and P1_3, (B) P2_1, P2_2, P2_3, (C) P3_1, P3_2, P3_3, and (D) P4_1, P4_2, P4_3 using agar well diffusion assay. C: crude protein of untransformed E. coli T7 Express LysY/Iq (New England BioLabs, Inc). S: cell-free culture supernatant of P. polymyxa Kp10. Inhibition zones were obtained in all of the recombinant proteins, thus confirming their antilisterial activity.
Article Snippet: The genes encoding for P1, P2, P3, and P4 were separately cloned into pET28b+ for expression in E. coli T7 Express LysY/Iq (New England BioLabs).
Techniques: In Vitro, Activity Assay, Recombinant, Diffusion-based Assay, Inhibition