e coli strains top10  (Thermo Fisher)


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    Structured Review

    Thermo Fisher e coli strains top10
    Effect of sorbitol on growth of E. coli <t>Top10</t> transformed with LEA genes coding for cytoplasmic (clone E1C) and mitochondrial (clone 4A1) LEA proteins. Cells were grown for 24 h in LB medium, then equal amounts of cells were added to fresh LB
    E Coli Strains Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strains top10/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    e coli strains top10 - by Bioz Stars, 2020-01
    99/100 stars

    Images

    1) Product Images from "Study of model systems to test the potential function of Artemia group 1 late embryogenesis abundant (LEA) proteins"

    Article Title: Study of model systems to test the potential function of Artemia group 1 late embryogenesis abundant (LEA) proteins

    Journal: Cell Stress & Chaperones

    doi: 10.1007/s12192-015-0647-3

    Effect of sorbitol on growth of E. coli Top10 transformed with LEA genes coding for cytoplasmic (clone E1C) and mitochondrial (clone 4A1) LEA proteins. Cells were grown for 24 h in LB medium, then equal amounts of cells were added to fresh LB
    Figure Legend Snippet: Effect of sorbitol on growth of E. coli Top10 transformed with LEA genes coding for cytoplasmic (clone E1C) and mitochondrial (clone 4A1) LEA proteins. Cells were grown for 24 h in LB medium, then equal amounts of cells were added to fresh LB

    Techniques Used: Transformation Assay

    Growth of E. coli transformed with different plasmid constructs. a Growth of E. coli Top10 after 22–23 h incubation at 37 °C in LB medium. pCR2.1 clone with the empty vector, B25 control clone with E1A sequence in reverse
    Figure Legend Snippet: Growth of E. coli transformed with different plasmid constructs. a Growth of E. coli Top10 after 22–23 h incubation at 37 °C in LB medium. pCR2.1 clone with the empty vector, B25 control clone with E1A sequence in reverse

    Techniques Used: Transformation Assay, Plasmid Preparation, Construct, Incubation, Sequencing

    2) Product Images from "RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer"

    Article Title: RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer

    Journal: Inflammatory bowel diseases

    doi: 10.1097/MIB.0b013e318281f2fd

    RNase-L mediates IFNβ production by BMDM in response to bacterial RNA. (A) BMDM were infected with Top10 or LF82 strains of E. coli at an MOI of 5 or 20 for 8 hours and IFNβ was measured by ELISA. (B) BMDMs were transfected with E. coli
    Figure Legend Snippet: RNase-L mediates IFNβ production by BMDM in response to bacterial RNA. (A) BMDM were infected with Top10 or LF82 strains of E. coli at an MOI of 5 or 20 for 8 hours and IFNβ was measured by ELISA. (B) BMDMs were transfected with E. coli

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Transfection

    Related Articles

    Clone Assay:

    Article Title: Discovery of new enzymes and metabolic pathways using structure and genome context
    Article Snippet: .. E. coli strain TOP10 (Invitrogen) was used for plasmid maintenance, propagation and cloning purpose. .. E. coli strain S17-1 was used for conjugation .

    Article Title: A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
    Article Snippet: General methods and materials A BrBphP gene was ordered from GeneScript and cloned into a modified pBAD vector containing the heme oxygenase-1 gene from cyanobacteria. .. Libraries were expressed in E. coli strain TOP10 (Invitrogen) and screened by imaging the agar plates with colonies using a BioSpectrum Imaging System (UVP).

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: .. E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction. .. All plasmid constructs were confirmed by using PCR amplification, restriction digestion, and sequence analysis.

    Article Title: Rgg-Shp regulators are important for pneumococcal colonization and invasion through their effect on mannose utilization and capsule synthesis
    Article Snippet: .. Escherichia coli strains Top10 (Invitrogen) and DH5α were used for cloning and were grown in Luria broth (LB) or on Luria broth agar with kanamycin (150 μg/ml) or ampicillin (100 μg/ml). .. Synthetic peptides were used to test the activity of Shp144 and Shp939.

    Article Title: Role of the Two Catalytic Domains of DSR-E Dextransucrase and Their Involvement in the Formation of Highly ?-1,2 Branched Dextran
    Article Snippet: .. E. coli strain TOP10 was used as the host for TOPO cloning of PCR products in pBAD/TOPO Thiofusion (Invitrogen) and for the overproduction of complete ( ) and truncated DSR-E enzymes. .. Luria-Bertani broth was used as the culture medium, and growth was monitored at 600 nm with a Shimadzu UV-1601 spectrophotometer.

    Article Title: Identification and Characterization of trans-Isopentenyl Diphosphate Synthases Involved in Herbivory-Induced Volatile Terpene Formation in Populus trichocarpa
    Article Snippet: The full-length ORF of PtFPPS1 and the N-terminal truncated ORFs of PtGPPS1.LSU, PtGPPS2.LSU, PtGPPS.SSU1 , and PtGPPS.SSU2 lacking the putative signal peptides predicted with the programs ChloroP ( http://www.cbs.dtu.dk/services/ChloroP/ ), TargetP ( http://www.cbs.dtu.dk/services/TargetP/ ), and PREDOTAR ( https://urgi.versailles.inra.fr/Tools/Predotar ) ( ) were amplified from cDNA made from herbivore-damaged P. trichocarpa leaves using the primers listed in and cloned into the expression vectors pASK-IBA33+ (IBA-GmbH, Göttingen, Germany, PtGPPS1.LSU, PtGPPS2.LSU, PtGPPS.SSU1 , and PtGPPS.SSU2 ) or pASK-IBA37+ (PtFPPS1 ). .. The constructs were introduced into the E. coli strain TOP10 (Thermo Fisher Scientific, Erlangen, Germany) and fully sequenced to check for amplification errors.

    Amplification:

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: .. Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The expression vector for secretion of recombinant PfCyRPA was generated by PCR using the plasmid BVM_PFD1130W_FLAG_GP_His as template ( ).

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction. .. All plasmid constructs were confirmed by using PCR amplification, restriction digestion, and sequence analysis.

    Article Title: The Mating Type Switch-Activating Protein Sap1 Is Required for Replication Fork Arrest at the rRNA Genes of Fission Yeast
    Article Snippet: The sap1 gene was obtained by PCR amplification from genomic DNA using the primers SapEcoHis and SapHind (Table ). .. The plasmid obtained was used to transform the E. coli strain TOP10 (Invitrogen), and the expression of Sap1p was induced by the addition of 0.02% arabinose to exponentially growing cells for 2 h. The total proteins from 2.5 × 108 cells were separated in 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred to polyvinylidene difluoride membranes, and His6 -Sap1p was detected by using anti-His6 -peroxidase antibody (Roche).

    Article Title: Identification and Characterization of trans-Isopentenyl Diphosphate Synthases Involved in Herbivory-Induced Volatile Terpene Formation in Populus trichocarpa
    Article Snippet: .. The constructs were introduced into the E. coli strain TOP10 (Thermo Fisher Scientific, Erlangen, Germany) and fully sequenced to check for amplification errors. .. Heterologous Expression and Enzyme Assays Liquid cultures of bacteria harboring the expression constructs were grown at 18 °C to an OD600 of 0.5.

    Construct:

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: The isogenic bosR mutant OY08 was constructed in our previous study (Ouyang et al., ). .. E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction.

    Article Title: Identification and Characterization of trans-Isopentenyl Diphosphate Synthases Involved in Herbivory-Induced Volatile Terpene Formation in Populus trichocarpa
    Article Snippet: .. The constructs were introduced into the E. coli strain TOP10 (Thermo Fisher Scientific, Erlangen, Germany) and fully sequenced to check for amplification errors. .. Heterologous Expression and Enzyme Assays Liquid cultures of bacteria harboring the expression constructs were grown at 18 °C to an OD600 of 0.5.

    Article Title: Production optimization of cyanophycinase ChpEal from Pseudomonas alcaligenes DIP1
    Article Snippet: To construct the P. alcaligenes DIP1 DNA library, genomic DNA of P. alcaligenes DIP1 was digested with Pst I and ligated into the vector pBluescriptSK- (Stratagene). .. E. coli strain Top10 (Invitrogen) and plasmid pBBR1MCS-4 ( ) were used to subclone and sequence DNA fragments containing cphEal .

    Enzyme-linked Immunosorbent Assay:

    Article Title: RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer
    Article Snippet: Paragraph title: Single ELISA assay for IFNβ ... E. coli strains TOP10 (Life Technologies) or LF82 were grown to exponential phase in L broth and used at multiplicities of infections of 5 and 20 to infect bone marrow macrophages in bone marrow cultured media (in the absence of antibiotics) for 1.5 hr after which gentamycin (Life Technologies) was added to the media at a concentration of 100 μg/ml.

    Expressing:

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The expression vector for secretion of recombinant PfCyRPA was generated by PCR using the plasmid BVM_PFD1130W_FLAG_GP_His as template ( ).

    Article Title: The Mating Type Switch-Activating Protein Sap1 Is Required for Replication Fork Arrest at the rRNA Genes of Fission Yeast
    Article Snippet: .. The plasmid obtained was used to transform the E. coli strain TOP10 (Invitrogen), and the expression of Sap1p was induced by the addition of 0.02% arabinose to exponentially growing cells for 2 h. The total proteins from 2.5 × 108 cells were separated in 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred to polyvinylidene difluoride membranes, and His6 -Sap1p was detected by using anti-His6 -peroxidase antibody (Roche). .. The 78-bp fragment containing RFB1 and analyzed in the mobility shift assay (Fig. ) was used to purify Sap1p by affinity chromatography.

    Article Title: Identification and Characterization of trans-Isopentenyl Diphosphate Synthases Involved in Herbivory-Induced Volatile Terpene Formation in Populus trichocarpa
    Article Snippet: The full-length ORF of PtFPPS1 and the N-terminal truncated ORFs of PtGPPS1.LSU, PtGPPS2.LSU, PtGPPS.SSU1 , and PtGPPS.SSU2 lacking the putative signal peptides predicted with the programs ChloroP ( http://www.cbs.dtu.dk/services/ChloroP/ ), TargetP ( http://www.cbs.dtu.dk/services/TargetP/ ), and PREDOTAR ( https://urgi.versailles.inra.fr/Tools/Predotar ) ( ) were amplified from cDNA made from herbivore-damaged P. trichocarpa leaves using the primers listed in and cloned into the expression vectors pASK-IBA33+ (IBA-GmbH, Göttingen, Germany, PtGPPS1.LSU, PtGPPS2.LSU, PtGPPS.SSU1 , and PtGPPS.SSU2 ) or pASK-IBA37+ (PtFPPS1 ). .. The constructs were introduced into the E. coli strain TOP10 (Thermo Fisher Scientific, Erlangen, Germany) and fully sequenced to check for amplification errors.

    Article Title: Production optimization of cyanophycinase ChpEal from Pseudomonas alcaligenes DIP1
    Article Snippet: E. coli strain Top10 (Invitrogen) and plasmid pBBR1MCS-4 ( ) were used to subclone and sequence DNA fragments containing cphEal . .. Nucleic acid sequence data and deduced amino acid sequences were analyzed using BLAST , Genamics Expression 1.1 and SignalP 3.0 ( ) server.

    Modification:

    Article Title: A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
    Article Snippet: General methods and materials A BrBphP gene was ordered from GeneScript and cloned into a modified pBAD vector containing the heme oxygenase-1 gene from cyanobacteria. .. Libraries were expressed in E. coli strain TOP10 (Invitrogen) and screened by imaging the agar plates with colonies using a BioSpectrum Imaging System (UVP).

    Derivative Assay:

    Article Title: Molecular Basis for the Differential Expression of the Global Regulator VieA in Vibrio cholerae Biotypes Directed by H-NS, LeuO and Quorum Sensing
    Article Snippet: V. cholerae mutants used in this study were derived from the classical and El Tor biotype strains O395 and C7258, respectively. .. Escherichia coli strains TOP10 (Life Technologies) or SM10λpir ( ) were used for plasmid propagation.

    Conjugation Assay:

    Article Title: Discovery of new enzymes and metabolic pathways using structure and genome context
    Article Snippet: E. coli strain TOP10 (Invitrogen) was used for plasmid maintenance, propagation and cloning purpose. .. E. coli strain S17-1 was used for conjugation .

    Transfection:

    Article Title: RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer
    Article Snippet: Following transfections, cell supernatants were collected and used to measure IFNβ with VeriKine TM Mouse Interferon-Beta ELISA KIT (PBL InterferonSource, Piscataway, NJ). .. E. coli strains TOP10 (Life Technologies) or LF82 were grown to exponential phase in L broth and used at multiplicities of infections of 5 and 20 to infect bone marrow macrophages in bone marrow cultured media (in the absence of antibiotics) for 1.5 hr after which gentamycin (Life Technologies) was added to the media at a concentration of 100 μg/ml.

    Cell Culture:

    Article Title: A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
    Article Snippet: Libraries were expressed in E. coli strain TOP10 (Invitrogen) and screened by imaging the agar plates with colonies using a BioSpectrum Imaging System (UVP). .. Cultured cells were not tested for the presence of Mycoplasma, as such contamination would not impact the conclu- sions made on the basis of our imaging results.

    Article Title: Evolution of high-level ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-β-d-arabinose biosynthetic and utilization pathway genes
    Article Snippet: M. tuberculosis strains were cultured as described previously . .. For all plasmid construction, E. coli strains Top10 (Invitrogen) were grown in Luria-Bertani medium supplemented with 50 µg/ml kanamycin, where appropriate.

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: Escherichia coli strains were cultured in lysogeny broth (LB) medium supplemented with appropriate antibiotics at the following concentrations: ampicillin, 100 μg/ml; kanamycin, 50 μg/ml; or spectinomycin, 100 μg/ml. .. E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction.

    Article Title: Role of the Two Catalytic Domains of DSR-E Dextransucrase and Their Involvement in the Formation of Highly ?-1,2 Branched Dextran
    Article Snippet: E. coli strain TOP10 was used as the host for TOPO cloning of PCR products in pBAD/TOPO Thiofusion (Invitrogen) and for the overproduction of complete ( ) and truncated DSR-E enzymes. .. L. mesenteroides NRRL strain B-1299 was cultured in 40 g of sucrose liter−1 , 20 g of yeast extract liter−1 , 20 g of K2 HPO4 (pH 6.9) liter−1 (adjusted with orthophosphoric acid), and 0.01 g of MnSO4 (1H2 O) liter−1 at 30°C.

    Article Title: RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer
    Article Snippet: .. E. coli strains TOP10 (Life Technologies) or LF82 were grown to exponential phase in L broth and used at multiplicities of infections of 5 and 20 to infect bone marrow macrophages in bone marrow cultured media (in the absence of antibiotics) for 1.5 hr after which gentamycin (Life Technologies) was added to the media at a concentration of 100 μg/ml. .. Cell supernatants were used for IFNβ determination by ELISA.

    Generated:

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The expression vector for secretion of recombinant PfCyRPA was generated by PCR using the plasmid BVM_PFD1130W_FLAG_GP_His as template ( ).

    Imaging:

    Article Title: A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
    Article Snippet: .. Libraries were expressed in E. coli strain TOP10 (Invitrogen) and screened by imaging the agar plates with colonies using a BioSpectrum Imaging System (UVP). .. Cultured cells were not tested for the presence of Mycoplasma, as such contamination would not impact the conclu- sions made on the basis of our imaging results.

    Sequencing:

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The resulting expression vector pcDNA3.1_BVM_CyRPA(26-362)_His6 encodes the bee-venom melittin (BVM) signal sequence to secrete PfCyRPA into the cultivation medium, and a C-terminal His6 -tag.

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction. .. All plasmid constructs were confirmed by using PCR amplification, restriction digestion, and sequence analysis.

    Article Title: Production optimization of cyanophycinase ChpEal from Pseudomonas alcaligenes DIP1
    Article Snippet: .. E. coli strain Top10 (Invitrogen) and plasmid pBBR1MCS-4 ( ) were used to subclone and sequence DNA fragments containing cphEal . .. Sequence analysis was performed by Seqlab (Göttingen, Germany).

    Recombinant:

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The expression vector for secretion of recombinant PfCyRPA was generated by PCR using the plasmid BVM_PFD1130W_FLAG_GP_His as template ( ).

    Nucleic Acid Electrophoresis:

    Article Title: Study of model systems to test the potential function of Artemia group 1 late embryogenesis abundant (LEA) proteins
    Article Snippet: All gel electrophoresis materials were from Bio-Rad (Mississauga, ON, Canada), while the X-ray film and developer were Kodak products from Ultident (St. Laurent, QC, Canada). .. Plasmids pCR2.1, pYES2, and pYES3 and E. coli strains Top10, Top10F’, INVαF’, and S. cerevisiae , strain INVSc1, were from Invitrogen (Carlsbad, CA, USA).

    Mutagenesis:

    Article Title: Discovery of new enzymes and metabolic pathways using structure and genome context
    Article Snippet: Bacterial Strains and Growth Conditions P. denitrificans PD1222 wild-type and mutant strains were grown in minimal medium containing (g per liter) K2 HPO4 , 6.0; 4.0 g of KH2 PO4 4;, sodium molybdate, 0,15; MgS04 · 7H2 0, 0.2, CaCl2, ; 0.04; MnSO4 · 2H2 O, 0.001; and FeSO4 · 7H2 0 1.1 g with or without 1.6 g of NH4 Cl as nitrogen source. .. E. coli strain TOP10 (Invitrogen) was used for plasmid maintenance, propagation and cloning purpose.

    Article Title: A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
    Article Snippet: Random mutagenesis was performed using a GeneMorph II Random Mutagenesis Kit (Stratagene). .. Libraries were expressed in E. coli strain TOP10 (Invitrogen) and screened by imaging the agar plates with colonies using a BioSpectrum Imaging System (UVP).

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: The isogenic bosR mutant OY08 was constructed in our previous study (Ouyang et al., ). .. E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction.

    Isolation:

    Article Title: Identification and Characterization of trans-Isopentenyl Diphosphate Synthases Involved in Herbivory-Induced Volatile Terpene Formation in Populus trichocarpa
    Article Snippet: Paragraph title: 4.3. Identification and Isolation of trans-IDS Genes ... The constructs were introduced into the E. coli strain TOP10 (Thermo Fisher Scientific, Erlangen, Germany) and fully sequenced to check for amplification errors.

    Article Title: Production optimization of cyanophycinase ChpEal from Pseudomonas alcaligenes DIP1
    Article Snippet: Paragraph title: Isolation, manipulation and analysis of DNA ... E. coli strain Top10 (Invitrogen) and plasmid pBBR1MCS-4 ( ) were used to subclone and sequence DNA fragments containing cphEal .

    Microscopy:

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: Growth of B. burgdorferi was monitored by dark-field microscopy. .. E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction.

    Polymerase Chain Reaction:

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The expression vector for secretion of recombinant PfCyRPA was generated by PCR using the plasmid BVM_PFD1130W_FLAG_GP_His as template ( ).

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction. .. All plasmid constructs were confirmed by using PCR amplification, restriction digestion, and sequence analysis.

    Article Title: The Mating Type Switch-Activating Protein Sap1 Is Required for Replication Fork Arrest at the rRNA Genes of Fission Yeast
    Article Snippet: The PCR product was digested with EcoRI and HindIII to generate sticky ends and inserted into the expression vector pBAD24 (Invitrogen) digested with the same enzymes. .. The plasmid obtained was used to transform the E. coli strain TOP10 (Invitrogen), and the expression of Sap1p was induced by the addition of 0.02% arabinose to exponentially growing cells for 2 h. The total proteins from 2.5 × 108 cells were separated in 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred to polyvinylidene difluoride membranes, and His6 -Sap1p was detected by using anti-His6 -peroxidase antibody (Roche).

    Article Title: Role of the Two Catalytic Domains of DSR-E Dextransucrase and Their Involvement in the Formation of Highly ?-1,2 Branched Dextran
    Article Snippet: .. E. coli strain TOP10 was used as the host for TOPO cloning of PCR products in pBAD/TOPO Thiofusion (Invitrogen) and for the overproduction of complete ( ) and truncated DSR-E enzymes. .. Luria-Bertani broth was used as the culture medium, and growth was monitored at 600 nm with a Shimadzu UV-1601 spectrophotometer.

    Plasmid Preparation:

    Article Title: Discovery of new enzymes and metabolic pathways using structure and genome context
    Article Snippet: .. E. coli strain TOP10 (Invitrogen) was used for plasmid maintenance, propagation and cloning purpose. .. E. coli strain S17-1 was used for conjugation .

    Article Title: A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
    Article Snippet: General methods and materials A BrBphP gene was ordered from GeneScript and cloned into a modified pBAD vector containing the heme oxygenase-1 gene from cyanobacteria. .. Libraries were expressed in E. coli strain TOP10 (Invitrogen) and screened by imaging the agar plates with colonies using a BioSpectrum Imaging System (UVP).

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The expression vector for secretion of recombinant PfCyRPA was generated by PCR using the plasmid BVM_PFD1130W_FLAG_GP_His as template ( ).

    Article Title: Evolution of high-level ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-β-d-arabinose biosynthetic and utilization pathway genes
    Article Snippet: .. For all plasmid construction, E. coli strains Top10 (Invitrogen) were grown in Luria-Bertani medium supplemented with 50 µg/ml kanamycin, where appropriate. .. Clinical M. tuberculosis strains with varying ethambutol MICs were selected from a pedigreed collection of 229 highly characterized M. tuberculosis isolates established by the World Health Organization Special Programme for Research and Training in Tropical Disease using geographic, phylogenetic and ethambutol MIC diversity as selection criteria .

    Article Title: The CXXC Motifs Are Essential for the Function of BosR in Borrelia burgdorferi
    Article Snippet: .. E. coli strain TOP10 (Thermo Fisher Scientific, Grand Island, NY) was used as the cloning host for plasmid construction. .. All plasmid constructs were confirmed by using PCR amplification, restriction digestion, and sequence analysis.

    Article Title: Molecular Basis for the Differential Expression of the Global Regulator VieA in Vibrio cholerae Biotypes Directed by H-NS, LeuO and Quorum Sensing
    Article Snippet: .. Escherichia coli strains TOP10 (Life Technologies) or SM10λpir ( ) were used for plasmid propagation. ..

    Article Title: The Mating Type Switch-Activating Protein Sap1 Is Required for Replication Fork Arrest at the rRNA Genes of Fission Yeast
    Article Snippet: .. The plasmid obtained was used to transform the E. coli strain TOP10 (Invitrogen), and the expression of Sap1p was induced by the addition of 0.02% arabinose to exponentially growing cells for 2 h. The total proteins from 2.5 × 108 cells were separated in 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred to polyvinylidene difluoride membranes, and His6 -Sap1p was detected by using anti-His6 -peroxidase antibody (Roche). .. The 78-bp fragment containing RFB1 and analyzed in the mobility shift assay (Fig. ) was used to purify Sap1p by affinity chromatography.

    Article Title: Production optimization of cyanophycinase ChpEal from Pseudomonas alcaligenes DIP1
    Article Snippet: .. E. coli strain Top10 (Invitrogen) and plasmid pBBR1MCS-4 ( ) were used to subclone and sequence DNA fragments containing cphEal . .. Sequence analysis was performed by Seqlab (Göttingen, Germany).

    Article Title: A toxin antitoxin system promotes the maintenance of the IncA/C-mobilizable Salmonella Genomic Island 1
    Article Snippet: E. coli strain TOP10 was used for the PSK assays (Invitrogen SARL, Cergy-Pontoise, France). .. Plasmid R55 was used as the reference IncA/C plasmid in SGI1 stability and compatibility tests.

    Selection:

    Article Title: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
    Article Snippet: .. Molecular biology Plasmids were amplified in E. coli strain Top10 (Life Technologies) grown in LB medium under 100 μg/mL ampicillin selection. .. The expression vector for secretion of recombinant PfCyRPA was generated by PCR using the plasmid BVM_PFD1130W_FLAG_GP_His as template ( ).

    Article Title: Evolution of high-level ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-β-d-arabinose biosynthetic and utilization pathway genes
    Article Snippet: For all plasmid construction, E. coli strains Top10 (Invitrogen) were grown in Luria-Bertani medium supplemented with 50 µg/ml kanamycin, where appropriate. .. Clinical M. tuberculosis strains with varying ethambutol MICs were selected from a pedigreed collection of 229 highly characterized M. tuberculosis isolates established by the World Health Organization Special Programme for Research and Training in Tropical Disease using geographic, phylogenetic and ethambutol MIC diversity as selection criteria .

    Spectrophotometry:

    Article Title: Role of the Two Catalytic Domains of DSR-E Dextransucrase and Their Involvement in the Formation of Highly ?-1,2 Branched Dextran
    Article Snippet: E. coli strain TOP10 was used as the host for TOPO cloning of PCR products in pBAD/TOPO Thiofusion (Invitrogen) and for the overproduction of complete ( ) and truncated DSR-E enzymes. .. Luria-Bertani broth was used as the culture medium, and growth was monitored at 600 nm with a Shimadzu UV-1601 spectrophotometer.

    Concentration Assay:

    Article Title: Discovery of new enzymes and metabolic pathways using structure and genome context
    Article Snippet: P. denitrificans PD1222 was grown aerobically at 30°C in mineral medium supplemented with either glucose/succinate, t/c-Hyp-B or methanol at the same concentration (20 mM). .. E. coli strain TOP10 (Invitrogen) was used for plasmid maintenance, propagation and cloning purpose.

    Article Title: RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer
    Article Snippet: .. E. coli strains TOP10 (Life Technologies) or LF82 were grown to exponential phase in L broth and used at multiplicities of infections of 5 and 20 to infect bone marrow macrophages in bone marrow cultured media (in the absence of antibiotics) for 1.5 hr after which gentamycin (Life Technologies) was added to the media at a concentration of 100 μg/ml. .. Cell supernatants were used for IFNβ determination by ELISA.

    Standard Deviation:

    Article Title: Evolution of high-level ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-β-d-arabinose biosynthetic and utilization pathway genes
    Article Snippet: Because each value within a triplicate MIC test was almost always identical to the other values within the same triplicate set, a single MIC value is shown without standard deviation for each test unless otherwise indicated. .. For all plasmid construction, E. coli strains Top10 (Invitrogen) were grown in Luria-Bertani medium supplemented with 50 µg/ml kanamycin, where appropriate.

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    Thermo Fisher one shot top10 chemically competent e coli
    One Shot Top10 Chemically Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot top10 chemically competent e coli/product/Thermo Fisher
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    one shot top10 chemically competent e coli - by Bioz Stars, 2020-01
    90/100 stars
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    MultiShot StripWell TOP10 chemically competent E coli cells are cloning competent cells that packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium throughput bacterial
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