e coli strains top10  (Thermo Fisher)


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    Structured Review

    Thermo Fisher e coli strains top10
    RNase-L mediates IFNβ production by BMDM in response to bacterial RNA. (A) BMDM were infected with <t>Top10</t> or LF82 strains of E. coli at an MOI of 5 or 20 for 8 hours and IFNβ was measured by ELISA. (B) BMDMs were transfected with E. coli
    E Coli Strains Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strains top10/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    e coli strains top10 - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer"

    Article Title: RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer

    Journal: Inflammatory bowel diseases

    doi: 10.1097/MIB.0b013e318281f2fd

    RNase-L mediates IFNβ production by BMDM in response to bacterial RNA. (A) BMDM were infected with Top10 or LF82 strains of E. coli at an MOI of 5 or 20 for 8 hours and IFNβ was measured by ELISA. (B) BMDMs were transfected with E. coli
    Figure Legend Snippet: RNase-L mediates IFNβ production by BMDM in response to bacterial RNA. (A) BMDM were infected with Top10 or LF82 strains of E. coli at an MOI of 5 or 20 for 8 hours and IFNβ was measured by ELISA. (B) BMDMs were transfected with E. coli

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Transfection

    2) Product Images from "Study of model systems to test the potential function of Artemia group 1 late embryogenesis abundant (LEA) proteins"

    Article Title: Study of model systems to test the potential function of Artemia group 1 late embryogenesis abundant (LEA) proteins

    Journal: Cell Stress & Chaperones

    doi: 10.1007/s12192-015-0647-3

    Effect of sorbitol on growth of E. coli Top10 transformed with LEA genes coding for cytoplasmic (clone E1C) and mitochondrial (clone 4A1) LEA proteins. Cells were grown for 24 h in LB medium, then equal amounts of cells were added to fresh LB
    Figure Legend Snippet: Effect of sorbitol on growth of E. coli Top10 transformed with LEA genes coding for cytoplasmic (clone E1C) and mitochondrial (clone 4A1) LEA proteins. Cells were grown for 24 h in LB medium, then equal amounts of cells were added to fresh LB

    Techniques Used: Transformation Assay

    Growth of E. coli transformed with different plasmid constructs. a Growth of E. coli Top10 after 22–23 h incubation at 37 °C in LB medium. pCR2.1 clone with the empty vector, B25 control clone with E1A sequence in reverse
    Figure Legend Snippet: Growth of E. coli transformed with different plasmid constructs. a Growth of E. coli Top10 after 22–23 h incubation at 37 °C in LB medium. pCR2.1 clone with the empty vector, B25 control clone with E1A sequence in reverse

    Techniques Used: Transformation Assay, Plasmid Preparation, Construct, Incubation, Sequencing

    Related Articles

    Electroporation:

    Article Title: Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains
    Article Snippet: .. One Shot TOP10 Electrocomp Escherichia coli ( E. coli) was from Invitrogen (cat. no. C404052, Carlsbad, CA, USA) and used for DNA transformation by electroporation, which was carried out in a 2 mm cuvette in the following conditions: 2500 V, 25 μF, 200 Ω. .. The DNA-transformed E. coli was spread onto lysogeny broth (LB) plate containing 100 μg/mL of Ampicillin with about 20-h incubation at 30 °C.

    Flow Cytometry:

    Article Title: Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay.
    Article Snippet: .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis.

    Over Expression:

    Article Title: UvrC Coordinates an O2-Sensitive [4Fe4S] Cofactor
    Article Snippet: .. A starter culture of One Shot TOP10 Electrocomp E. coli Cells (Invitrogen) containing the UvrC overexpression plasmid (encoding the His6 -MBP-UvrC fusion protein) or Cys→Ala mutant overexpression plasmids were grown (200 rpm, 37 °C) ~16 h in LB/Amp (50 mg/L). .. Large (1 L) cultures LB/Amp (50 mg/L) were inoculated with starter culture and grown (225 rpm, 37 °C) to an OD600 of ~0.6, and expression was induced by addition of arabinose to a final concentration of 10 mg/L.

    Clone Assay:

    Article Title: Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay.
    Article Snippet: .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis.

    Article Title: Insulin-like Growth Factor-binding Protein-5-induced Laminin ?1 Transcription Requires Filamin A *
    Article Snippet: .. The cloning reaction was transformed into One Shot TOP10 Escherichia coli (Invitrogen). .. Multiple clones were screened by PCR using GFP vector sequencing primers provided with the vector.

    Article Title: Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo
    Article Snippet: .. The PCR products were then cloned into pCRII-TOPO and transformed in One Shot TOP10 E . coli (Thermo Scientific) according to manufacturer’s instructions. .. The plasmids were purified and the insert was sequenced using M13 sequencing primers.

    Mutagenesis:

    Article Title: UvrC Coordinates an O2-Sensitive [4Fe4S] Cofactor
    Article Snippet: .. A starter culture of One Shot TOP10 Electrocomp E. coli Cells (Invitrogen) containing the UvrC overexpression plasmid (encoding the His6 -MBP-UvrC fusion protein) or Cys→Ala mutant overexpression plasmids were grown (200 rpm, 37 °C) ~16 h in LB/Amp (50 mg/L). .. Large (1 L) cultures LB/Amp (50 mg/L) were inoculated with starter culture and grown (225 rpm, 37 °C) to an OD600 of ~0.6, and expression was induced by addition of arabinose to a final concentration of 10 mg/L.

    Construct:

    Article Title: Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site
    Article Snippet: .. One Shot TOP 10 E.coli cells (Invitrogen) were transformed with the recombinant prodomain construct. .. The expression of the recombinant protein was induced with 1 mM isopropyl-1-thio-β-D-galactopyranoside.

    Purification:

    Article Title: Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay.
    Article Snippet: .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis.

    Polymerase Chain Reaction:

    Article Title: Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo
    Article Snippet: .. The PCR products were then cloned into pCRII-TOPO and transformed in One Shot TOP10 E . coli (Thermo Scientific) according to manufacturer’s instructions. .. The plasmids were purified and the insert was sequenced using M13 sequencing primers.

    Transformation Assay:

    Article Title: Resolving Cytosolic Diffusive States in Bacteria by Single-Molecule Tracking
    Article Snippet: .. Digested vector and inserts were ligated using T4 DNA ligase and transformed into E. coli TOP10 cells. .. Colonies were PCR screened for the presence of the correct insert using GoTaq DNA Polymerase (Thermo Fisher Scientific, Hampton, NH), and the plasmid was isolated from positive clones (Omega Bio-tek, Norcross, GA).

    Article Title: Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains
    Article Snippet: .. One Shot TOP10 Electrocomp Escherichia coli ( E. coli) was from Invitrogen (cat. no. C404052, Carlsbad, CA, USA) and used for DNA transformation by electroporation, which was carried out in a 2 mm cuvette in the following conditions: 2500 V, 25 μF, 200 Ω. .. The DNA-transformed E. coli was spread onto lysogeny broth (LB) plate containing 100 μg/mL of Ampicillin with about 20-h incubation at 30 °C.

    Article Title: Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay.
    Article Snippet: .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis.

    Article Title: Complementary expression of EphA7 and SCO-spondin during posterior commissure development
    Article Snippet: .. One Shot TOP10 Escherichia coli (Invitrogen, Carlsbad, CA, USA) were transformed with this vector. .. Posterior purification of the plasmid was performed with the E.Z.N.A.

    Article Title: Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site
    Article Snippet: .. One Shot TOP 10 E.coli cells (Invitrogen) were transformed with the recombinant prodomain construct. .. The expression of the recombinant protein was induced with 1 mM isopropyl-1-thio-β-D-galactopyranoside.

    Article Title: Insulin-like Growth Factor-binding Protein-5-induced Laminin ?1 Transcription Requires Filamin A *
    Article Snippet: .. The cloning reaction was transformed into One Shot TOP10 Escherichia coli (Invitrogen). .. Multiple clones were screened by PCR using GFP vector sequencing primers provided with the vector.

    Article Title: Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo
    Article Snippet: .. The PCR products were then cloned into pCRII-TOPO and transformed in One Shot TOP10 E . coli (Thermo Scientific) according to manufacturer’s instructions. .. The plasmids were purified and the insert was sequenced using M13 sequencing primers.

    Recombinant:

    Article Title: Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site
    Article Snippet: .. One Shot TOP 10 E.coli cells (Invitrogen) were transformed with the recombinant prodomain construct. .. The expression of the recombinant protein was induced with 1 mM isopropyl-1-thio-β-D-galactopyranoside.

    Plasmid Preparation:

    Article Title: Resolving Cytosolic Diffusive States in Bacteria by Single-Molecule Tracking
    Article Snippet: .. Digested vector and inserts were ligated using T4 DNA ligase and transformed into E. coli TOP10 cells. .. Colonies were PCR screened for the presence of the correct insert using GoTaq DNA Polymerase (Thermo Fisher Scientific, Hampton, NH), and the plasmid was isolated from positive clones (Omega Bio-tek, Norcross, GA).

    Article Title: UvrC Coordinates an O2-Sensitive [4Fe4S] Cofactor
    Article Snippet: .. A starter culture of One Shot TOP10 Electrocomp E. coli Cells (Invitrogen) containing the UvrC overexpression plasmid (encoding the His6 -MBP-UvrC fusion protein) or Cys→Ala mutant overexpression plasmids were grown (200 rpm, 37 °C) ~16 h in LB/Amp (50 mg/L). .. Large (1 L) cultures LB/Amp (50 mg/L) were inoculated with starter culture and grown (225 rpm, 37 °C) to an OD600 of ~0.6, and expression was induced by addition of arabinose to a final concentration of 10 mg/L.

    Article Title: Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay.
    Article Snippet: .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. .. The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis.

    Article Title: Complementary expression of EphA7 and SCO-spondin during posterior commissure development
    Article Snippet: .. One Shot TOP10 Escherichia coli (Invitrogen, Carlsbad, CA, USA) were transformed with this vector. .. Posterior purification of the plasmid was performed with the E.Z.N.A.

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    Thermo Fisher one shot top10 cells
    One Shot Top10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot top10 cells/product/Thermo Fisher
    Average 99 stars, based on 33 article reviews
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    97
    Thermo Fisher multishot flexplate top10 competent cells
    Analysis of the pop transcriptional unit. (A) Promoter activity assay for the promoter of pop , which was introduced in the promoterless GFP vector pProbe-NT. Mean fluorescence units of E. coli <t>Top10</t> cells with the different constructs in culture conditions as indicated are given. Error bars show standard deviations. Statistical significance between empty vector (grey bars) and the promoter construct (blue bars) and between growth conditions was tested with a Welch two sample t-test (**/++ p≤0.01; *** p≤0.001; ns, not significant). (B) Test for mono- or polycistronic mRNA. An agarose gel of RT-PCRs is shown. Two different forward primers, binding within ycbG (no. 23) or within pop (no. 24), were combined with a pop reverse primer (no. 25). L: 100 bp DNA Ladder (NEB); 23: PCR with primers 23+25; 24: PCR with primers 24+25. (C) Test for the predicted rho-independent terminator. An agarose gel of RT-PCRs is shown. Two different reverse primers, binding upstream (no. 27) or downstream (no. 28) of the stem loop structure, were combined with a pop forward primer (no. 26). L, 1 kb DNA Ladder (NEB); 27, PCR with primers 27+26; 28, PCR with primers 28+26; d. ORFs, downstream ORFs.
    Multishot Flexplate Top10 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multishot flexplate top10 competent cells/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
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    88
    Thermo Fisher growth conditions escherichia coli top10
    Analysis of the pop transcriptional unit. (A) Promoter activity assay for the promoter of pop , which was introduced in the promoterless GFP vector pProbe-NT. Mean fluorescence units of E. coli <t>Top10</t> cells with the different constructs in culture conditions as indicated are given. Error bars show standard deviations. Statistical significance between empty vector (grey bars) and the promoter construct (blue bars) and between growth conditions was tested with a Welch two sample t-test (**/++ p≤0.01; *** p≤0.001; ns, not significant). (B) Test for mono- or polycistronic mRNA. An agarose gel of RT-PCRs is shown. Two different forward primers, binding within ycbG (no. 23) or within pop (no. 24), were combined with a pop reverse primer (no. 25). L: 100 bp DNA Ladder (NEB); 23: PCR with primers 23+25; 24: PCR with primers 24+25. (C) Test for the predicted rho-independent terminator. An agarose gel of RT-PCRs is shown. Two different reverse primers, binding upstream (no. 27) or downstream (no. 28) of the stem loop structure, were combined with a pop forward primer (no. 26). L, 1 kb DNA Ladder (NEB); 27, PCR with primers 27+26; 28, PCR with primers 28+26; d. ORFs, downstream ORFs.
    Growth Conditions Escherichia Coli Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/growth conditions escherichia coli top10/product/Thermo Fisher
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    growth conditions escherichia coli top10 - by Bioz Stars, 2020-09
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    Image Search Results


    Analysis of the pop transcriptional unit. (A) Promoter activity assay for the promoter of pop , which was introduced in the promoterless GFP vector pProbe-NT. Mean fluorescence units of E. coli Top10 cells with the different constructs in culture conditions as indicated are given. Error bars show standard deviations. Statistical significance between empty vector (grey bars) and the promoter construct (blue bars) and between growth conditions was tested with a Welch two sample t-test (**/++ p≤0.01; *** p≤0.001; ns, not significant). (B) Test for mono- or polycistronic mRNA. An agarose gel of RT-PCRs is shown. Two different forward primers, binding within ycbG (no. 23) or within pop (no. 24), were combined with a pop reverse primer (no. 25). L: 100 bp DNA Ladder (NEB); 23: PCR with primers 23+25; 24: PCR with primers 24+25. (C) Test for the predicted rho-independent terminator. An agarose gel of RT-PCRs is shown. Two different reverse primers, binding upstream (no. 27) or downstream (no. 28) of the stem loop structure, were combined with a pop forward primer (no. 26). L, 1 kb DNA Ladder (NEB); 27, PCR with primers 27+26; 28, PCR with primers 28+26; d. ORFs, downstream ORFs.

    Journal: bioRxiv

    Article Title: A novel pH-regulated, unusual 603 bp overlapping protein coding gene pop is encoded antisense to ompA in Escherichia coli O157:H7 (EHEC)

    doi: 10.1101/852251

    Figure Lengend Snippet: Analysis of the pop transcriptional unit. (A) Promoter activity assay for the promoter of pop , which was introduced in the promoterless GFP vector pProbe-NT. Mean fluorescence units of E. coli Top10 cells with the different constructs in culture conditions as indicated are given. Error bars show standard deviations. Statistical significance between empty vector (grey bars) and the promoter construct (blue bars) and between growth conditions was tested with a Welch two sample t-test (**/++ p≤0.01; *** p≤0.001; ns, not significant). (B) Test for mono- or polycistronic mRNA. An agarose gel of RT-PCRs is shown. Two different forward primers, binding within ycbG (no. 23) or within pop (no. 24), were combined with a pop reverse primer (no. 25). L: 100 bp DNA Ladder (NEB); 23: PCR with primers 23+25; 24: PCR with primers 24+25. (C) Test for the predicted rho-independent terminator. An agarose gel of RT-PCRs is shown. Two different reverse primers, binding upstream (no. 27) or downstream (no. 28) of the stem loop structure, were combined with a pop forward primer (no. 26). L, 1 kb DNA Ladder (NEB); 27, PCR with primers 27+26; 28, PCR with primers 28+26; d. ORFs, downstream ORFs.

    Article Snippet: Vector constructs were transformed in E. coli Top10 cells and plated on LB with required antibiotics.

    Techniques: Activity Assay, Plasmid Preparation, Fluorescence, Construct, Agarose Gel Electrophoresis, Binding Assay, Polymerase Chain Reaction