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Stratagene e coli strain bl21 gold
E Coli Strain Bl21 Gold, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli strain bl21 gold/product/Stratagene
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
e coli strain bl21 gold - by Bioz Stars, 2020-08
88/100 stars

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Transduction:

Article Title: Pervasive Cryptic Epistasis in Molecular Evolution
Article Snippet: .. A derivative of E. coli strain BL21-gold (Stratagene) was constructed by P1 transduction of the ΔleuB-leuC::kanr construct from strain JW5807. ..

Article Title: Pervasive Cryptic Epistasis in Molecular Evolution
Article Snippet: .. Protein Expression Mutant IMDHs were over-expressed from plasmids in a derivative of E. coli strain BL21-gold (Stratagene) formed by P1 transduction of the ΔleuB-leuC::kanr construct from strain JW5807. .. Transformed cells were grown overnight at 37°C in 5 mL of LB containing ampicillin (100 µg/ml) and 0.2 mM IPTG.

Clone Assay:

Article Title: The Ca2+/Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development
Article Snippet: .. This cDNA fragment (∼1.2 kb) was cloned into the Nde I/ Bam HI site of the E. coli expression vector pET15b (Novagen), selected by transformation into E. coli strain DH5α, and then introduced into E. coli strain BL21-gold(DE3) (Stratagene). ..

Mutagenesis:

Article Title: Pervasive Cryptic Epistasis in Molecular Evolution
Article Snippet: .. Protein Expression Mutant IMDHs were over-expressed from plasmids in a derivative of E. coli strain BL21-gold (Stratagene) formed by P1 transduction of the ΔleuB-leuC::kanr construct from strain JW5807. .. Transformed cells were grown overnight at 37°C in 5 mL of LB containing ampicillin (100 µg/ml) and 0.2 mM IPTG.

Construct:

Article Title: Pervasive Cryptic Epistasis in Molecular Evolution
Article Snippet: .. A derivative of E. coli strain BL21-gold (Stratagene) was constructed by P1 transduction of the ΔleuB-leuC::kanr construct from strain JW5807. ..

Article Title: Recombinant prion protein vaccination of transgenic elk PrP mice and reindeer overcomes self-tolerance and protects mice against chronic wasting disease
Article Snippet: .. Briefly, pQE30 (Qiagen) expression vector was used to express the monomeric and dimeric constructs in Escherichia coli strain BL21-Gold(DE3) pLysS (Stratagene) as described previously ( , ). .. Nickel-nitrilotriacetic acid Superflow resin beads (Qiagen) were used to separate His-tagged immunogens from the bacterial lysate.

Article Title: Pervasive Cryptic Epistasis in Molecular Evolution
Article Snippet: .. Protein Expression Mutant IMDHs were over-expressed from plasmids in a derivative of E. coli strain BL21-gold (Stratagene) formed by P1 transduction of the ΔleuB-leuC::kanr construct from strain JW5807. .. Transformed cells were grown overnight at 37°C in 5 mL of LB containing ampicillin (100 µg/ml) and 0.2 mM IPTG.

Expressing:

Article Title: Recombinant prion protein vaccination of transgenic elk PrP mice and reindeer overcomes self-tolerance and protects mice against chronic wasting disease
Article Snippet: .. Briefly, pQE30 (Qiagen) expression vector was used to express the monomeric and dimeric constructs in Escherichia coli strain BL21-Gold(DE3) pLysS (Stratagene) as described previously ( , ). .. Nickel-nitrilotriacetic acid Superflow resin beads (Qiagen) were used to separate His-tagged immunogens from the bacterial lysate.

Article Title: Pervasive Cryptic Epistasis in Molecular Evolution
Article Snippet: .. Protein Expression Mutant IMDHs were over-expressed from plasmids in a derivative of E. coli strain BL21-gold (Stratagene) formed by P1 transduction of the ΔleuB-leuC::kanr construct from strain JW5807. .. Transformed cells were grown overnight at 37°C in 5 mL of LB containing ampicillin (100 µg/ml) and 0.2 mM IPTG.

Article Title: The Carboxy-Terminal Region of the Human Immunodeficiency Virus Type 1 Protein Rev Has Multiple Roles in Mediating CRM1-Related Rev Functions
Article Snippet: .. The expression of recombinant zz-Rev protein was induced by 0.5 mM isopropyl-β- d -thiogalactopyranoside for 15 h at 20°C in the Escherichia coli strain BL21-Gold(DE3)pLysS (Stratagene). .. E. coli cells were lysed in buffer A (50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM MgCl2 , 2 mM dithiothreitol, and 1 μg each of aprotinin, leupeptin, and pepstatin per ml) with a French press after three freeze-thaw cycles and clarified by centrifugation (100,000 × g , 1 h).

Article Title: The Ca2+/Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development
Article Snippet: .. This cDNA fragment (∼1.2 kb) was cloned into the Nde I/ Bam HI site of the E. coli expression vector pET15b (Novagen), selected by transformation into E. coli strain DH5α, and then introduced into E. coli strain BL21-gold(DE3) (Stratagene). ..

Article Title: Immunization of cervidized transgenic mice with multimeric deer prion protein induces self-antibodies that antagonize chronic wasting disease infectivity in vitro
Article Snippet: .. Expression of immunogens was performed in Escherichia coli strain BL21-Gold(DE3) pLysS (Stratagene) as previously described , . .. Briefly, the bacterial pellet was lysed with lysis buffer pH 7.8 (6 M guanidinium hydrochloride [GdnHCl], 20 mM sodium-phosphate, 500 mM NaCl).

Transformation Assay:

Article Title: The Ca2+/Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development
Article Snippet: .. This cDNA fragment (∼1.2 kb) was cloned into the Nde I/ Bam HI site of the E. coli expression vector pET15b (Novagen), selected by transformation into E. coli strain DH5α, and then introduced into E. coli strain BL21-gold(DE3) (Stratagene). ..

Recombinant:

Article Title: The Carboxy-Terminal Region of the Human Immunodeficiency Virus Type 1 Protein Rev Has Multiple Roles in Mediating CRM1-Related Rev Functions
Article Snippet: .. The expression of recombinant zz-Rev protein was induced by 0.5 mM isopropyl-β- d -thiogalactopyranoside for 15 h at 20°C in the Escherichia coli strain BL21-Gold(DE3)pLysS (Stratagene). .. E. coli cells were lysed in buffer A (50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM MgCl2 , 2 mM dithiothreitol, and 1 μg each of aprotinin, leupeptin, and pepstatin per ml) with a French press after three freeze-thaw cycles and clarified by centrifugation (100,000 × g , 1 h).

Plasmid Preparation:

Article Title: Mechanisms of Checkpoint Kinase Rad53 Inactivation after a Double-Strand Break in Saccharomyces cerevisiae ▿
Article Snippet: .. E. coli strain BL21-Gold(DE3) (Stratagene) carrying the pETM-30/FHA1(1-164) plasmid was grown in M9 minimal medium supplemented with (15 NH4 )2 SO4 (0.5 g liter−1 ; Eurisotop) as the sole nitrogen source. .. After IPTG (isopropyl-1-thio-β-D-galactopyranoside) induction at an optical density of 0.8, cells were lysed by freeze-thaw and disrupted by sonication.

Article Title: Recombinant prion protein vaccination of transgenic elk PrP mice and reindeer overcomes self-tolerance and protects mice against chronic wasting disease
Article Snippet: .. Briefly, pQE30 (Qiagen) expression vector was used to express the monomeric and dimeric constructs in Escherichia coli strain BL21-Gold(DE3) pLysS (Stratagene) as described previously ( , ). .. Nickel-nitrilotriacetic acid Superflow resin beads (Qiagen) were used to separate His-tagged immunogens from the bacterial lysate.

Article Title: The Ca2+/Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development
Article Snippet: .. This cDNA fragment (∼1.2 kb) was cloned into the Nde I/ Bam HI site of the E. coli expression vector pET15b (Novagen), selected by transformation into E. coli strain DH5α, and then introduced into E. coli strain BL21-gold(DE3) (Stratagene). ..

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    Stratagene e coli strains bl21 de3 gold
    Expression in whole cell lysates: (A) Growth in an automated plate reader of E. coli <t>BL21</t> (DE3) <t>Gold</t> expressing 48 different pBAD constructs (left panel) and 48 different pET constructs (Right panel). Arrows indicate time of induction. (B) Dot blot analysis
    E Coli Strains Bl21 De3 Gold, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strains bl21 de3 gold/product/Stratagene
    Average 85 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    e coli strains bl21 de3 gold - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    91
    Stratagene bl21 gold
    Optimization of expression conditions and screening background. (A) Colonies of E . coli <t>BL21</t> transformed with TN-XXL cloned into the vector pRSETB (exposure time 4s, gain 2x, binning 1). Scale bar, 10 mm (B) Colonies of E . coli XL1 transformed with TN-XXL cloned into pRSETB and imaged under the same conditions. (C) Mean fluorescence intensities ± SD of colonies transformed with TN-XXL in either BL1 or XL1, imaged under identical conditions (n BL21 = 312, n XL1 = 558). (D) ΔR/R ± SD of E . coli colonies expressing TN-XXL cloned into BL21 or XL1 (n BL21 = 19, n XL1 = 15). (E) Auto-fluorescence of agar plate versus white blotting paper imaged under identical conditions with filters used for FRET imaging. Error bars indicate SD. (F) Basal ratio valuesR 0 ± SD of XL1 colonies transformed with the FRET sensor TN-XXL and imaged on agar plate or on filter paper. (n plate = 149 colonies, n blotting paper = 90 colonies) (G) ΔR/R ± SD of the same colonies following calcium application.
    Bl21 Gold, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 gold/product/Stratagene
    Average 91 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    bl21 gold - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Expression in whole cell lysates: (A) Growth in an automated plate reader of E. coli BL21 (DE3) Gold expressing 48 different pBAD constructs (left panel) and 48 different pET constructs (Right panel). Arrows indicate time of induction. (B) Dot blot analysis

    Journal: Journal of molecular biology

    Article Title: The funnel approach to the pre-crystallization production of membrane proteins

    doi: 10.1016/j.jmb.2007.12.059

    Figure Lengend Snippet: Expression in whole cell lysates: (A) Growth in an automated plate reader of E. coli BL21 (DE3) Gold expressing 48 different pBAD constructs (left panel) and 48 different pET constructs (Right panel). Arrows indicate time of induction. (B) Dot blot analysis

    Article Snippet: E. coli strains BL21 (DE3) GOLD and BL21(DE3)RIPL were from Stratagene.

    Techniques: Expressing, Construct, Positron Emission Tomography, Dot Blot

    Optimization of expression conditions and screening background. (A) Colonies of E . coli BL21 transformed with TN-XXL cloned into the vector pRSETB (exposure time 4s, gain 2x, binning 1). Scale bar, 10 mm (B) Colonies of E . coli XL1 transformed with TN-XXL cloned into pRSETB and imaged under the same conditions. (C) Mean fluorescence intensities ± SD of colonies transformed with TN-XXL in either BL1 or XL1, imaged under identical conditions (n BL21 = 312, n XL1 = 558). (D) ΔR/R ± SD of E . coli colonies expressing TN-XXL cloned into BL21 or XL1 (n BL21 = 19, n XL1 = 15). (E) Auto-fluorescence of agar plate versus white blotting paper imaged under identical conditions with filters used for FRET imaging. Error bars indicate SD. (F) Basal ratio valuesR 0 ± SD of XL1 colonies transformed with the FRET sensor TN-XXL and imaged on agar plate or on filter paper. (n plate = 149 colonies, n blotting paper = 90 colonies) (G) ΔR/R ± SD of the same colonies following calcium application.

    Journal: PLoS ONE

    Article Title: Large Scale Bacterial Colony Screening of Diversified FRET Biosensors

    doi: 10.1371/journal.pone.0119860

    Figure Lengend Snippet: Optimization of expression conditions and screening background. (A) Colonies of E . coli BL21 transformed with TN-XXL cloned into the vector pRSETB (exposure time 4s, gain 2x, binning 1). Scale bar, 10 mm (B) Colonies of E . coli XL1 transformed with TN-XXL cloned into pRSETB and imaged under the same conditions. (C) Mean fluorescence intensities ± SD of colonies transformed with TN-XXL in either BL1 or XL1, imaged under identical conditions (n BL21 = 312, n XL1 = 558). (D) ΔR/R ± SD of E . coli colonies expressing TN-XXL cloned into BL21 or XL1 (n BL21 = 19, n XL1 = 15). (E) Auto-fluorescence of agar plate versus white blotting paper imaged under identical conditions with filters used for FRET imaging. Error bars indicate SD. (F) Basal ratio valuesR 0 ± SD of XL1 colonies transformed with the FRET sensor TN-XXL and imaged on agar plate or on filter paper. (n plate = 149 colonies, n blotting paper = 90 colonies) (G) ΔR/R ± SD of the same colonies following calcium application.

    Article Snippet: We compared sensor response in a bacterial strain used for protein expression, BL21-Gold (Stratagene), and a strain used for cloning, XL1-Blue (Stratagene) ( ).

    Techniques: Expressing, Transformation Assay, Clone Assay, Plasmid Preparation, Fluorescence, Imaging