e coli j5 lps extracts  (ATCC)


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    ATCC e coli j5 lps extracts
    Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes
    E Coli J5 Lps Extracts, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Model architectures for bacterial membranes"

    Article Title: Model architectures for bacterial membranes

    Journal: Biophysical Reviews

    doi: 10.1007/s12551-021-00913-7

    Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes
    Figure Legend Snippet: Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes

    Techniques Used: Microarray, Sterility, Activation Assay, Activity Assay, Mutagenesis, Permeability, Fluorescence, Translocation Assay, Binding Assay

    Summary of cited solid supported bilayer models, the lipid source, the lipid species utilised and their corresponding research outcomes
    Figure Legend Snippet: Summary of cited solid supported bilayer models, the lipid source, the lipid species utilised and their corresponding research outcomes

    Techniques Used: Activity Assay, Derivative Assay, In Vitro, Binding Assay, Functional Assay, Diffusion-based Assay, Generated

    Summary of cited computational models, the bacterial species modelled, and the lipid species utilised and their corresponding research outcomes
    Figure Legend Snippet: Summary of cited computational models, the bacterial species modelled, and the lipid species utilised and their corresponding research outcomes

    Techniques Used: Binding Assay, Introduce, Electroporation, Permeability, Spectroscopy, Modification, Sublimation, Construct, Diffusion-based Assay, Functional Assay

    e coli j5 lps  (ATCC)


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    ATCC e coli j5 lps
    SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— <t>E.</t> <t>coli</t> 0119; 6— E. coli <t>J5;</t> 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; <t>11—LPS-MW</t> Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
    E Coli J5 Lps, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification"

    Article Title: Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25073927

    SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— E. coli 0119; 6— E. coli J5; 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; 11—LPS-MW Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
    Figure Legend Snippet: SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— E. coli 0119; 6— E. coli J5; 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; 11—LPS-MW Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.

    Techniques Used: SDS Page, Staining

    MALDI mass spectra obtained for tetra-acyl E. coli J5 lipid A ( A ) and hexa-acyl E. coli J5 lipid A ( B ). Corresponding lipid A structures ( C ). Comparison of LAL and HEK-blue hTLR-4 activities ( D ). Fatty acids other than 14:0-3-OH are presented with different colors.
    Figure Legend Snippet: MALDI mass spectra obtained for tetra-acyl E. coli J5 lipid A ( A ) and hexa-acyl E. coli J5 lipid A ( B ). Corresponding lipid A structures ( C ). Comparison of LAL and HEK-blue hTLR-4 activities ( D ). Fatty acids other than 14:0-3-OH are presented with different colors.

    Techniques Used: Comparison

    e coli 43745 j5  (ATCC)


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    ATCC e coli 43745 j5
    E Coli 43745 J5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli j5 lps extracts  (ATCC)


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    ATCC e coli j5 lps extracts
    Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes
    E Coli J5 Lps Extracts, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Model architectures for bacterial membranes"

    Article Title: Model architectures for bacterial membranes

    Journal: Biophysical Reviews

    doi: 10.1007/s12551-021-00913-7

    Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes
    Figure Legend Snippet: Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes

    Techniques Used: Microarray, Sterility, Activation Assay, Activity Assay, Mutagenesis, Permeability, Fluorescence, Translocation Assay, Binding Assay

    Summary of cited solid supported bilayer models, the lipid source, the lipid species utilised and their corresponding research outcomes
    Figure Legend Snippet: Summary of cited solid supported bilayer models, the lipid source, the lipid species utilised and their corresponding research outcomes

    Techniques Used: Activity Assay, Derivative Assay, In Vitro, Binding Assay, Functional Assay, Diffusion-based Assay, Generated

    Summary of cited computational models, the bacterial species modelled, and the lipid species utilised and their corresponding research outcomes
    Figure Legend Snippet: Summary of cited computational models, the bacterial species modelled, and the lipid species utilised and their corresponding research outcomes

    Techniques Used: Binding Assay, Introduce, Electroporation, Permeability, Spectroscopy, Modification, Sublimation, Construct, Diffusion-based Assay, Functional Assay

    e coli strains j5 3  (ATCC)


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    ATCC e coli strains j5 3
    E Coli Strains J5 3, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli 43745 j5  (ATCC)


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    ATCC e coli 43745 j5
    E Coli 43745 J5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli j5  (ATCC)


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    ATCC e coli j5
    Cytokines secreted by THP-1 cells stimulated by LPSs from various E. cloacae complex strains 10 6 THP-1 cells were stimulated for 24 hr with 1 ng/mL of purified LPS from different E. cloacae complex strains or from <t>E.</t> <t>coli</t> <t>J5.</t> (A) IL-6 production. (B) TNF-α production. Results are expressed as the mean and standard deviation of 9 values (Three independent experiments in triplicate). Significance was determined using the GraphPad Prism8 software, by two-tailed Student's t-test. ∗: p < 0.0001.
    E Coli J5, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92/100 stars

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    1) Product Images from "Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock"

    Article Title: Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock

    Journal: iScience

    doi: 10.1016/j.isci.2021.102916

    Cytokines secreted by THP-1 cells stimulated by LPSs from various E. cloacae complex strains 10 6 THP-1 cells were stimulated for 24 hr with 1 ng/mL of purified LPS from different E. cloacae complex strains or from E. coli J5. (A) IL-6 production. (B) TNF-α production. Results are expressed as the mean and standard deviation of 9 values (Three independent experiments in triplicate). Significance was determined using the GraphPad Prism8 software, by two-tailed Student's t-test. ∗: p < 0.0001.
    Figure Legend Snippet: Cytokines secreted by THP-1 cells stimulated by LPSs from various E. cloacae complex strains 10 6 THP-1 cells were stimulated for 24 hr with 1 ng/mL of purified LPS from different E. cloacae complex strains or from E. coli J5. (A) IL-6 production. (B) TNF-α production. Results are expressed as the mean and standard deviation of 9 values (Three independent experiments in triplicate). Significance was determined using the GraphPad Prism8 software, by two-tailed Student's t-test. ∗: p < 0.0001.

    Techniques Used: Purification, Standard Deviation, Software, Two Tailed Test

    e coli j5  (ATCC)


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    ATCC e coli j5
    Analyses of purified LPS from the two V. filiformis samples compared with LPS standards by SDS-PAGE (A) and HPTLC (B). A: 1, <t>E.</t> <t>coli</t> <t>J5</t> LPS; 2, V. filiformis V.f5-6 LPS; 3, V. filiformis V.f6+2PEA LPS; 4, B. pertussis 1414 LPS; 5, P. aeruginosa serotype O6 LPS. One-tenth of a microgram of each LPS was loaded per well for the SDS-PAGE electrophoresis. B: 1, B. pertussis 1414 LPS; 2, E. coli J5; 3, V. filiformis V.f5-6; 4, V. filiformis V.f6+2PEA. Twenty micrograms were deposited for HPTLC migration.
    E Coli J5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies"

    Article Title: Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M072900

    Analyses of purified LPS from the two V. filiformis samples compared with LPS standards by SDS-PAGE (A) and HPTLC (B). A: 1, E. coli J5 LPS; 2, V. filiformis V.f5-6 LPS; 3, V. filiformis V.f6+2PEA LPS; 4, B. pertussis 1414 LPS; 5, P. aeruginosa serotype O6 LPS. One-tenth of a microgram of each LPS was loaded per well for the SDS-PAGE electrophoresis. B: 1, B. pertussis 1414 LPS; 2, E. coli J5; 3, V. filiformis V.f5-6; 4, V. filiformis V.f6+2PEA. Twenty micrograms were deposited for HPTLC migration.
    Figure Legend Snippet: Analyses of purified LPS from the two V. filiformis samples compared with LPS standards by SDS-PAGE (A) and HPTLC (B). A: 1, E. coli J5 LPS; 2, V. filiformis V.f5-6 LPS; 3, V. filiformis V.f6+2PEA LPS; 4, B. pertussis 1414 LPS; 5, P. aeruginosa serotype O6 LPS. One-tenth of a microgram of each LPS was loaded per well for the SDS-PAGE electrophoresis. B: 1, B. pertussis 1414 LPS; 2, E. coli J5; 3, V. filiformis V.f5-6; 4, V. filiformis V.f6+2PEA. Twenty micrograms were deposited for HPTLC migration.

    Techniques Used: Purification, SDS Page, High Performance Thin Layer Chromatography, Electrophoresis, Migration

    Lipid A structures of the major molecular species present in the purified LPS samples. The structures presented with the corresponding MWT given masses correspond to published data for all LPSs. The dashed lines indicate elements missing in the structures for each LPS sample used in this paper and corresponding to MWS. E. coli J5 (A), H. influenzae (B), R. gelatinosa (C), P. aeruginosa (D), N. meningitis (E), B. pertussis 1414 (F), B. pertussis 18-323 (G), V. filiformis V.f5-6 (H), V. filiformis V.f6+2PEA (I).
    Figure Legend Snippet: Lipid A structures of the major molecular species present in the purified LPS samples. The structures presented with the corresponding MWT given masses correspond to published data for all LPSs. The dashed lines indicate elements missing in the structures for each LPS sample used in this paper and corresponding to MWS. E. coli J5 (A), H. influenzae (B), R. gelatinosa (C), P. aeruginosa (D), N. meningitis (E), B. pertussis 1414 (F), B. pertussis 18-323 (G), V. filiformis V.f5-6 (H), V. filiformis V.f6+2PEA (I).

    Techniques Used: Purification

    ROS production and caspase 3/7 activation induced by LPS stimulation. Cell viability (A) and caspase 3/7 activation (B) were assessed after 24 h THP-1 stimulation. C: ROS production by THP-1 cells after 1 h stimulation. N-acetyl-l-cysteine was used to inhibit ROS production in E. coli J5 stimulation. *P < 0.05 compared to no LPS.
    Figure Legend Snippet: ROS production and caspase 3/7 activation induced by LPS stimulation. Cell viability (A) and caspase 3/7 activation (B) were assessed after 24 h THP-1 stimulation. C: ROS production by THP-1 cells after 1 h stimulation. N-acetyl-l-cysteine was used to inhibit ROS production in E. coli J5 stimulation. *P < 0.05 compared to no LPS.

    Techniques Used: Activation Assay

    e coli j5  (ATCC)


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    ATCC e coli j5
    Analyses of purified LPS from the two V. filiformis samples compared with LPS standards by SDS-PAGE (A) and HPTLC (B). A: 1, <t>E.</t> <t>coli</t> <t>J5</t> LPS; 2, V. filiformis V.f5-6 LPS; 3, V. filiformis V.f6+2PEA LPS; 4, B. pertussis 1414 LPS; 5, P. aeruginosa serotype O6 LPS. One-tenth of a microgram of each LPS was loaded per well for the SDS-PAGE electrophoresis. B: 1, B. pertussis 1414 LPS; 2, E. coli J5; 3, V. filiformis V.f5-6; 4, V. filiformis V.f6+2PEA. Twenty micrograms were deposited for HPTLC migration.
    E Coli J5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies"

    Article Title: Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M072900

    Analyses of purified LPS from the two V. filiformis samples compared with LPS standards by SDS-PAGE (A) and HPTLC (B). A: 1, E. coli J5 LPS; 2, V. filiformis V.f5-6 LPS; 3, V. filiformis V.f6+2PEA LPS; 4, B. pertussis 1414 LPS; 5, P. aeruginosa serotype O6 LPS. One-tenth of a microgram of each LPS was loaded per well for the SDS-PAGE electrophoresis. B: 1, B. pertussis 1414 LPS; 2, E. coli J5; 3, V. filiformis V.f5-6; 4, V. filiformis V.f6+2PEA. Twenty micrograms were deposited for HPTLC migration.
    Figure Legend Snippet: Analyses of purified LPS from the two V. filiformis samples compared with LPS standards by SDS-PAGE (A) and HPTLC (B). A: 1, E. coli J5 LPS; 2, V. filiformis V.f5-6 LPS; 3, V. filiformis V.f6+2PEA LPS; 4, B. pertussis 1414 LPS; 5, P. aeruginosa serotype O6 LPS. One-tenth of a microgram of each LPS was loaded per well for the SDS-PAGE electrophoresis. B: 1, B. pertussis 1414 LPS; 2, E. coli J5; 3, V. filiformis V.f5-6; 4, V. filiformis V.f6+2PEA. Twenty micrograms were deposited for HPTLC migration.

    Techniques Used: Purification, SDS Page, High Performance Thin Layer Chromatography, Electrophoresis, Migration

    Lipid A structures of the major molecular species present in the purified LPS samples. The structures presented with the corresponding MWT given masses correspond to published data for all LPSs. The dashed lines indicate elements missing in the structures for each LPS sample used in this paper and corresponding to MWS. E. coli J5 (A), H. influenzae (B), R. gelatinosa (C), P. aeruginosa (D), N. meningitis (E), B. pertussis 1414 (F), B. pertussis 18-323 (G), V. filiformis V.f5-6 (H), V. filiformis V.f6+2PEA (I).
    Figure Legend Snippet: Lipid A structures of the major molecular species present in the purified LPS samples. The structures presented with the corresponding MWT given masses correspond to published data for all LPSs. The dashed lines indicate elements missing in the structures for each LPS sample used in this paper and corresponding to MWS. E. coli J5 (A), H. influenzae (B), R. gelatinosa (C), P. aeruginosa (D), N. meningitis (E), B. pertussis 1414 (F), B. pertussis 18-323 (G), V. filiformis V.f5-6 (H), V. filiformis V.f6+2PEA (I).

    Techniques Used: Purification

    ROS production and caspase 3/7 activation induced by LPS stimulation. Cell viability (A) and caspase 3/7 activation (B) were assessed after 24 h THP-1 stimulation. C: ROS production by THP-1 cells after 1 h stimulation. N-acetyl-l-cysteine was used to inhibit ROS production in E. coli J5 stimulation. *P < 0.05 compared to no LPS.
    Figure Legend Snippet: ROS production and caspase 3/7 activation induced by LPS stimulation. Cell viability (A) and caspase 3/7 activation (B) were assessed after 24 h THP-1 stimulation. C: ROS production by THP-1 cells after 1 h stimulation. N-acetyl-l-cysteine was used to inhibit ROS production in E. coli J5 stimulation. *P < 0.05 compared to no LPS.

    Techniques Used: Activation Assay

    bacterial strains e coli j5  (ATCC)


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    ATCC bacterial strains e coli j5
    Bacterial Strains E Coli J5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli j5  (ATCC)


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    ATCC e coli j5
    E Coli J5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli j5 lps extracts
    Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes
    E Coli J5 Lps Extracts, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli j5 lps
    SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— <t>E.</t> <t>coli</t> 0119; 6— E. coli <t>J5;</t> 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; <t>11—LPS-MW</t> Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
    E Coli J5 Lps, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli 43745 j5
    SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— <t>E.</t> <t>coli</t> 0119; 6— E. coli <t>J5;</t> 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; <t>11—LPS-MW</t> Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
    E Coli 43745 J5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli strains j5 3
    SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— <t>E.</t> <t>coli</t> 0119; 6— E. coli <t>J5;</t> 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; <t>11—LPS-MW</t> Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
    E Coli Strains J5 3, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strains j5 3/product/ATCC
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    92
    ATCC e coli j5
    Cytokines secreted by THP-1 cells stimulated by LPSs from various E. cloacae complex strains 10 6 THP-1 cells were stimulated for 24 hr with 1 ng/mL of purified LPS from different E. cloacae complex strains or from <t>E.</t> <t>coli</t> <t>J5.</t> (A) IL-6 production. (B) TNF-α production. Results are expressed as the mean and standard deviation of 9 values (Three independent experiments in triplicate). Significance was determined using the GraphPad Prism8 software, by two-tailed Student's t-test. ∗: p < 0.0001.
    E Coli J5, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli j5/product/ATCC
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    ATCC bacterial strains e coli j5
    Cytokines secreted by THP-1 cells stimulated by LPSs from various E. cloacae complex strains 10 6 THP-1 cells were stimulated for 24 hr with 1 ng/mL of purified LPS from different E. cloacae complex strains or from <t>E.</t> <t>coli</t> <t>J5.</t> (A) IL-6 production. (B) TNF-α production. Results are expressed as the mean and standard deviation of 9 values (Three independent experiments in triplicate). Significance was determined using the GraphPad Prism8 software, by two-tailed Student's t-test. ∗: p < 0.0001.
    Bacterial Strains E Coli J5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes

    Journal: Biophysical Reviews

    Article Title: Model architectures for bacterial membranes

    doi: 10.1007/s12551-021-00913-7

    Figure Lengend Snippet: Summary of cited liposome models, the lipid source, the lipid species utilised, and their corresponding research outcomes

    Article Snippet: , Kubiak et al. ) , Purchased E. coli B (ATCC 11,303) polar lipid extracts, E. coli (O55:B5) LPS extracts, E. coli (EH-100) LPS extracts, E. coli (J5) LPS extracts, E. coli (F583) LPS, and lipid A extracts and synthesised lipids , Extracted: PE, PG, CL, S-LPS, FITC-LPS, Ra-LPS, Rc-LPS, Rd-LPS, MPLA Synthesised: Rhod-DHPE , Development of novel protocol for formation of GUVs composed of LPS species and E. coli extracts.

    Techniques: Microarray, Sterility, Activation Assay, Activity Assay, Mutagenesis, Permeability, Fluorescence, Translocation Assay, Binding Assay

    Summary of cited solid supported bilayer models, the lipid source, the lipid species utilised and their corresponding research outcomes

    Journal: Biophysical Reviews

    Article Title: Model architectures for bacterial membranes

    doi: 10.1007/s12551-021-00913-7

    Figure Lengend Snippet: Summary of cited solid supported bilayer models, the lipid source, the lipid species utilised and their corresponding research outcomes

    Article Snippet: , Kubiak et al. ) , Purchased E. coli B (ATCC 11,303) polar lipid extracts, E. coli (O55:B5) LPS extracts, E. coli (EH-100) LPS extracts, E. coli (J5) LPS extracts, E. coli (F583) LPS, and lipid A extracts and synthesised lipids , Extracted: PE, PG, CL, S-LPS, FITC-LPS, Ra-LPS, Rc-LPS, Rd-LPS, MPLA Synthesised: Rhod-DHPE , Development of novel protocol for formation of GUVs composed of LPS species and E. coli extracts.

    Techniques: Activity Assay, Derivative Assay, In Vitro, Binding Assay, Functional Assay, Diffusion-based Assay, Generated

    Summary of cited computational models, the bacterial species modelled, and the lipid species utilised and their corresponding research outcomes

    Journal: Biophysical Reviews

    Article Title: Model architectures for bacterial membranes

    doi: 10.1007/s12551-021-00913-7

    Figure Lengend Snippet: Summary of cited computational models, the bacterial species modelled, and the lipid species utilised and their corresponding research outcomes

    Article Snippet: , Kubiak et al. ) , Purchased E. coli B (ATCC 11,303) polar lipid extracts, E. coli (O55:B5) LPS extracts, E. coli (EH-100) LPS extracts, E. coli (J5) LPS extracts, E. coli (F583) LPS, and lipid A extracts and synthesised lipids , Extracted: PE, PG, CL, S-LPS, FITC-LPS, Ra-LPS, Rc-LPS, Rd-LPS, MPLA Synthesised: Rhod-DHPE , Development of novel protocol for formation of GUVs composed of LPS species and E. coli extracts.

    Techniques: Binding Assay, Introduce, Electroporation, Permeability, Spectroscopy, Modification, Sublimation, Construct, Diffusion-based Assay, Functional Assay

    SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— E. coli 0119; 6— E. coli J5; 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; 11—LPS-MW Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.

    Journal: International Journal of Molecular Sciences

    Article Title: Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification

    doi: 10.3390/ijms25073927

    Figure Lengend Snippet: SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— E. coli 0119; 6— E. coli J5; 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; 11—LPS-MW Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.

    Article Snippet: E. coli J5 LPS was extracted from strain ATCC 43745.

    Techniques: SDS Page, Staining

    MALDI mass spectra obtained for tetra-acyl E. coli J5 lipid A ( A ) and hexa-acyl E. coli J5 lipid A ( B ). Corresponding lipid A structures ( C ). Comparison of LAL and HEK-blue hTLR-4 activities ( D ). Fatty acids other than 14:0-3-OH are presented with different colors.

    Journal: International Journal of Molecular Sciences

    Article Title: Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification

    doi: 10.3390/ijms25073927

    Figure Lengend Snippet: MALDI mass spectra obtained for tetra-acyl E. coli J5 lipid A ( A ) and hexa-acyl E. coli J5 lipid A ( B ). Corresponding lipid A structures ( C ). Comparison of LAL and HEK-blue hTLR-4 activities ( D ). Fatty acids other than 14:0-3-OH are presented with different colors.

    Article Snippet: E. coli J5 LPS was extracted from strain ATCC 43745.

    Techniques: Comparison

    Cytokines secreted by THP-1 cells stimulated by LPSs from various E. cloacae complex strains 10 6 THP-1 cells were stimulated for 24 hr with 1 ng/mL of purified LPS from different E. cloacae complex strains or from E. coli J5. (A) IL-6 production. (B) TNF-α production. Results are expressed as the mean and standard deviation of 9 values (Three independent experiments in triplicate). Significance was determined using the GraphPad Prism8 software, by two-tailed Student's t-test. ∗: p < 0.0001.

    Journal: iScience

    Article Title: Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock

    doi: 10.1016/j.isci.2021.102916

    Figure Lengend Snippet: Cytokines secreted by THP-1 cells stimulated by LPSs from various E. cloacae complex strains 10 6 THP-1 cells were stimulated for 24 hr with 1 ng/mL of purified LPS from different E. cloacae complex strains or from E. coli J5. (A) IL-6 production. (B) TNF-α production. Results are expressed as the mean and standard deviation of 9 values (Three independent experiments in triplicate). Significance was determined using the GraphPad Prism8 software, by two-tailed Student's t-test. ∗: p < 0.0001.

    Article Snippet: The LPS extracted from E. coli J5 (ATCC, Old Town Manassas, VA) and purified as indicated above, was used as a reference.

    Techniques: Purification, Standard Deviation, Software, Two Tailed Test