Journal: PLoS ONE
Article Title: Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons
Figure Lengend Snippet: Cdk1 protein kinase activity measured from DCRNs in either the presence or absence of BDNF. ( A ) General cdk protein kinase activity, based on the phosphorylation of a Rb-derived peptide by the cdk activity present in cell extracts from DCRNs cultured for 20 h in the presence of 100 ng/ml NGF and treated for 30 min with either vehicle (Control) or 2 ng/ml BDNF. Equal amount of cell extracts were used in both cases. ( B ) Left panel, a representative western blot performed with an anti-cdk1 antibody using the cell extracts described above prior to immunoprecipitation (INPUT). Right panel, protein kinase activity immunoprecipitated with an anti cdk1 antibody (i.e. cdk1-specific kinase activity) from the cell extracts described above, normalized to the relative amount of cdk1 present in these extracts (see input in left panel). *p
Article Snippet: Cultures were maintained at 37°C for 20 h in a water-saturated atmosphere containing 5% CO2 and where stated, they were supplemented with recombinant NGF (Alomone Labs, and Sigma) and/or BDNF (Alomone Labs) for different time periods.
Techniques: Activity Assay, Derivative Assay, Cell Culture, Western Blot, Immunoprecipitation