Journal: Frontiers in Immunology

Article Title: Flagellar-based motility accelerates IgA-mediated agglutination of Salmonella Typhimurium at high bacterial cell densities

doi: 10.3389/fimmu.2023.1193855

Figure Lengend Snippet:

Article Snippet: E. coli DH5α competent cells were transformed with the resulting yhjH construct (NEB).

Techniques:

Journal: Cell

Article Title: Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome

doi: 10.1016/j.cell.2023.04.003

Figure Lengend Snippet: The COMMD proteins assemble into specific heteromeric complexes (A) Purification of COMMD sub-complexes. The ten human COMMD proteins were co-expressed in E. coli and purified via His-tags on different subunits followed by gel filtration. Peptide mass spectrometry identified the subunits co-purified with each tagged protein and reveals three distinct stable tetrameric complexes of COMMD1-6-4-8 (subcomplex A), COMMD2-3-4-8 (subcomplex B), and COMMD5-10-7-9 (subcomplex C). (B) 3.3-Å crystal structure of the tetrameric subcomplex C (COMMD5-10-7-9), primarily built around the three major binding interfaces shown in more detail below. (C) Key residues involved in the COMMD5-COMMD10 interface. (D) Key residues that form a β-sheet extension between COMMD5-COMMD10 and COMMD7-COMMD9 dimers. (E) Key COMMD10 residue Leu129 binds in a hydrophobic pocket to stabilize the tetramer. (F) The unique COMMD9 HN domain interface in which residues form stable and specific tetrameric interactions focusing on Trp157 of COMMD5. (G) Key interactions involving the COMMD9 linker between the HN and COMM domains centered around Ile118. (H) Similar to (F) showing the COMMD10 HN domain interactions with three subunits, centered on the COMMD7 Trp139 conserved sidechain.

Article Snippet: The bacterial expression plasmids were transformed into E. coli BL21 DE3 competent cells (New England Biolabs) and plated on agar plates containing ampicillin.

Techniques: Purification, Filtration, Mass Spectrometry, Binding Assay

Journal: Cell

Article Title: Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome

doi: 10.1016/j.cell.2023.04.003

Figure Lengend Snippet:

Article Snippet: The bacterial expression plasmids were transformed into E. coli BL21 DE3 competent cells (New England Biolabs) and plated on agar plates containing ampicillin.

Techniques: Strep-tag, FLAG-tag, Knock-Out, Transfection, Recombinant, Plasmid Preparation, Electron Microscopy, Sequencing, Software