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TransGen biotech co e coli bl21
Effect of Sip2 on bacterial resistance against serum damage. (A) Escherichia coli <t>BL21/pETSip2</t> (expressing Sip2) and BL21/pET259 (control) were treated with normal or inactivated tongue sole serum for 1 h, and bacterial survival was determined. (B) Edwardsiella tarda TX01, TX01Δ sip2 , and TX01Δ sip2 / sip2 were treated with tongue sole serum as above, and bacterial survival rate was determined. Data are the means of three independent experiments and presented as means ± SEM. Values with different letters indicate significantly different ( P
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1) Product Images from "Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection"

Article Title: Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.01084

Effect of Sip2 on bacterial resistance against serum damage. (A) Escherichia coli BL21/pETSip2 (expressing Sip2) and BL21/pET259 (control) were treated with normal or inactivated tongue sole serum for 1 h, and bacterial survival was determined. (B) Edwardsiella tarda TX01, TX01Δ sip2 , and TX01Δ sip2 / sip2 were treated with tongue sole serum as above, and bacterial survival rate was determined. Data are the means of three independent experiments and presented as means ± SEM. Values with different letters indicate significantly different ( P
Figure Legend Snippet: Effect of Sip2 on bacterial resistance against serum damage. (A) Escherichia coli BL21/pETSip2 (expressing Sip2) and BL21/pET259 (control) were treated with normal or inactivated tongue sole serum for 1 h, and bacterial survival was determined. (B) Edwardsiella tarda TX01, TX01Δ sip2 , and TX01Δ sip2 / sip2 were treated with tongue sole serum as above, and bacterial survival rate was determined. Data are the means of three independent experiments and presented as means ± SEM. Values with different letters indicate significantly different ( P

Techniques Used: Expressing

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Article Snippet: E. coli BL21 (DE3) and DH5α were purchased from TransGen Biotech (Beijing, China); E. coli S17-1λpir was purchased from Biomedal (Sevilla, Spain).

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Article Snippet: Expression of α -13 Giardin The recombinant plasmids were transformed into E. coli BL21(DE3) (TransGen Biotech, Beijing, China).

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Article Snippet: The recombinant plasmid was verified by DNA sequencing on a 3730XL system (Thermo Scientific) and transfected into E. coli BL21 (DE3; TransGen Biotech, Beijing, China).

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Article Snippet: Prokaryotic expression was performed in E. coli BL21(DE3) cells (TransGen Biotech, Beijing, China).

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Article Snippet: This gene was then cloned into the E1 plasmid (TransGen Biotech, Beijing, China), which has 18aa in upstream (MRGSHHHHHHGMASELAL), and transformed into E. coli BL21(DE3) cells (TransGen Biotech, Beijing, China).

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Article Snippet: To induce the expression of GhCYP-3 protein with a His-tag in E. coli BL21(DE3) (TransGen Biotech, Beijing, China), a final concentration of 1.0 mmol·L−1 isopropyl-β-D-thiogalactopyranoside (IPTG) was added into the culture when the OD600 value reached 0.4–0.6, and the culture was allowed to continue growing for 4 – 6 h before harvesting.

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Article Snippet: Therefore, to further confirm the invalidation of mcr-3-like in mediating colistin resistance, only the 1623-bp and 1626-bp coding sequences of mcr-3.3 and mcr-3-like were ligated into expression vector pET-28a (Novagen, Beijing, China) and transformed into E. coli BL21(DE3)plys (Transgen Biotech, Beijing, China).

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Article Snippet: E. coli strains and plasmids: E. coli BL21 (DE3) and E. coli DH5α (TransGen Biotech, China), pET-51b(+) vector (Novagen, USA), cloning vector pMD18-T (TaKaRa, China).

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    TransGen biotech co pgex 4t 2
    HPLC analysis after co-expression in E. coli of recombinant CaXMT1 andTCS1 by using pMAL-c5X vector instead of <t>pGEX-4t-2.</t> The top trace shows the negative control and the bottom trace shows the products of the sample enzymatic reaction. XR is substrate and SAM is added into the reaction as methyl donor.
    Pgex 4t 2, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co e coli bl21
    Effect of Sip2 on bacterial resistance against serum damage. (A) Escherichia coli <t>BL21/pETSip2</t> (expressing Sip2) and BL21/pET259 (control) were treated with normal or inactivated tongue sole serum for 1 h, and bacterial survival was determined. (B) Edwardsiella tarda TX01, TX01Δ sip2 , and TX01Δ sip2 / sip2 were treated with tongue sole serum as above, and bacterial survival rate was determined. Data are the means of three independent experiments and presented as means ± SEM. Values with different letters indicate significantly different ( P
    E Coli Bl21, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co bl21
    SDS-PAGE analysis of the CDA expression. E . coli <t>BL21</t> (DE3) cells containing pET-28a- cdd were grown and induced with 1 mM IPTG. The cells were sonicated and then centrifuged to divide into two fractions, soluble and insoluble fractions. Soluble fraction was then purified using Ni-NTA agarose. Lane 1, size markers; Lane 2, total proteins of the uninduced cells; Lane 3, total proteins of the IPTG-induced cells; Lane 4, purified protein of CDA.
    Bl21, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co e coli strain bl21
    Growth of E. coli expressing BjHMA4R . <t>BL21</t> transformants contained a pEASY-Blunt E1 expression vector (negative control) and BjHMA4R , respectively. The cultures were adjusted to an OD 600 of 1 and were serially diluted 10-fold in water. Five-microliter aliquots of each dilution were spotted either on nonselective LB plates or on LB plates supplemented with 600 μM CdCl 2 and 2.5 mM ZnCl 2, Ni(NO3) 2 , Co(NO3) 2 and Pb(NO3) 2 . After 1 day of incubation at 37 °C, the plates were imaged. The dilutions are indicated in the above figure, and three individual clones of each E. coli transformant were analyzed
    E Coli Strain Bl21, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HPLC analysis after co-expression in E. coli of recombinant CaXMT1 andTCS1 by using pMAL-c5X vector instead of pGEX-4t-2. The top trace shows the negative control and the bottom trace shows the products of the sample enzymatic reaction. XR is substrate and SAM is added into the reaction as methyl donor.

    Journal: PLoS ONE

    Article Title: Metabolic Engineering of Saccharomyces cerevisiae for Caffeine and Theobromine Production

    doi: 10.1371/journal.pone.0105368

    Figure Lengend Snippet: HPLC analysis after co-expression in E. coli of recombinant CaXMT1 andTCS1 by using pMAL-c5X vector instead of pGEX-4t-2. The top trace shows the negative control and the bottom trace shows the products of the sample enzymatic reaction. XR is substrate and SAM is added into the reaction as methyl donor.

    Article Snippet: For the further improvement, a new high efficiency expression vector, pMAL-c5X (NewEnglandBiolabs, Beijing, China) was used instead of pGEX-4t-2 (Transgen Biotech, Beijing, China) afterwards.

    Techniques: High Performance Liquid Chromatography, Expressing, Recombinant, Plasmid Preparation, Negative Control

    HPLC analysis after expression in E. coli of TCS1 or one of its putative active site mutants by using pMAL-c5X vector instead of pGEX-4t-2. (a) The trace shows the authentic standards run in parallel. (b) The trace shows the original TCS1 enzymatic reaction products. (c to f) show the HPLC analysis of the reaction products from the mutants TM1 to TM4. Black arrowheads indicate reaction products.

    Journal: PLoS ONE

    Article Title: Metabolic Engineering of Saccharomyces cerevisiae for Caffeine and Theobromine Production

    doi: 10.1371/journal.pone.0105368

    Figure Lengend Snippet: HPLC analysis after expression in E. coli of TCS1 or one of its putative active site mutants by using pMAL-c5X vector instead of pGEX-4t-2. (a) The trace shows the authentic standards run in parallel. (b) The trace shows the original TCS1 enzymatic reaction products. (c to f) show the HPLC analysis of the reaction products from the mutants TM1 to TM4. Black arrowheads indicate reaction products.

    Article Snippet: For the further improvement, a new high efficiency expression vector, pMAL-c5X (NewEnglandBiolabs, Beijing, China) was used instead of pGEX-4t-2 (Transgen Biotech, Beijing, China) afterwards.

    Techniques: High Performance Liquid Chromatography, Expressing, Plasmid Preparation

    HPLC analysis after expression in E. coli of TCS1 or one of its putative active site mutants by using pGEX-4t-2 vector. (a) The trace shows the authentic standards run in parallel. (b) The trace shows the original TCS1 enzymatic reaction products. (c to f) show the HPLC analysis of the reaction products from the mutants TM1 to TM4. Black arrowheads indicate reaction products.

    Journal: PLoS ONE

    Article Title: Metabolic Engineering of Saccharomyces cerevisiae for Caffeine and Theobromine Production

    doi: 10.1371/journal.pone.0105368

    Figure Lengend Snippet: HPLC analysis after expression in E. coli of TCS1 or one of its putative active site mutants by using pGEX-4t-2 vector. (a) The trace shows the authentic standards run in parallel. (b) The trace shows the original TCS1 enzymatic reaction products. (c to f) show the HPLC analysis of the reaction products from the mutants TM1 to TM4. Black arrowheads indicate reaction products.

    Article Snippet: For the further improvement, a new high efficiency expression vector, pMAL-c5X (NewEnglandBiolabs, Beijing, China) was used instead of pGEX-4t-2 (Transgen Biotech, Beijing, China) afterwards.

    Techniques: High Performance Liquid Chromatography, Expressing, Plasmid Preparation

    HPLC analysis after co-expression in E. coli of recombinant CaXMT1 andTCS1 by using pGEX-4t-2 vector. The top trace shows the negative control and the bottom trace shows the products of the sample enzymatic reaction. XR is substrate and SAM is added into the reaction as methyl donor.

    Journal: PLoS ONE

    Article Title: Metabolic Engineering of Saccharomyces cerevisiae for Caffeine and Theobromine Production

    doi: 10.1371/journal.pone.0105368

    Figure Lengend Snippet: HPLC analysis after co-expression in E. coli of recombinant CaXMT1 andTCS1 by using pGEX-4t-2 vector. The top trace shows the negative control and the bottom trace shows the products of the sample enzymatic reaction. XR is substrate and SAM is added into the reaction as methyl donor.

    Article Snippet: For the further improvement, a new high efficiency expression vector, pMAL-c5X (NewEnglandBiolabs, Beijing, China) was used instead of pGEX-4t-2 (Transgen Biotech, Beijing, China) afterwards.

    Techniques: High Performance Liquid Chromatography, Expressing, Recombinant, Plasmid Preparation, Negative Control

    Effect of Sip2 on bacterial resistance against serum damage. (A) Escherichia coli BL21/pETSip2 (expressing Sip2) and BL21/pET259 (control) were treated with normal or inactivated tongue sole serum for 1 h, and bacterial survival was determined. (B) Edwardsiella tarda TX01, TX01Δ sip2 , and TX01Δ sip2 / sip2 were treated with tongue sole serum as above, and bacterial survival rate was determined. Data are the means of three independent experiments and presented as means ± SEM. Values with different letters indicate significantly different ( P

    Journal: Frontiers in Microbiology

    Article Title: Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection

    doi: 10.3389/fmicb.2018.01084

    Figure Lengend Snippet: Effect of Sip2 on bacterial resistance against serum damage. (A) Escherichia coli BL21/pETSip2 (expressing Sip2) and BL21/pET259 (control) were treated with normal or inactivated tongue sole serum for 1 h, and bacterial survival was determined. (B) Edwardsiella tarda TX01, TX01Δ sip2 , and TX01Δ sip2 / sip2 were treated with tongue sole serum as above, and bacterial survival rate was determined. Data are the means of three independent experiments and presented as means ± SEM. Values with different letters indicate significantly different ( P

    Article Snippet: E. coli BL21 (DE3) and DH5α were purchased from TransGen Biotech (Beijing, China); E. coli S17-1λpir was purchased from Biomedal (Sevilla, Spain).

    Techniques: Expressing

    SDS-PAGE analysis of the CDA expression. E . coli BL21 (DE3) cells containing pET-28a- cdd were grown and induced with 1 mM IPTG. The cells were sonicated and then centrifuged to divide into two fractions, soluble and insoluble fractions. Soluble fraction was then purified using Ni-NTA agarose. Lane 1, size markers; Lane 2, total proteins of the uninduced cells; Lane 3, total proteins of the IPTG-induced cells; Lane 4, purified protein of CDA.

    Journal: PLoS ONE

    Article Title: An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains

    doi: 10.1371/journal.pone.0121612

    Figure Lengend Snippet: SDS-PAGE analysis of the CDA expression. E . coli BL21 (DE3) cells containing pET-28a- cdd were grown and induced with 1 mM IPTG. The cells were sonicated and then centrifuged to divide into two fractions, soluble and insoluble fractions. Soluble fraction was then purified using Ni-NTA agarose. Lane 1, size markers; Lane 2, total proteins of the uninduced cells; Lane 3, total proteins of the IPTG-induced cells; Lane 4, purified protein of CDA.

    Article Snippet: The host bacterial E . coli DH5α and BL21 (DE3) were purchased from Trans-Gen Biotech Company for the construction, propagation and expression of plasmids.

    Techniques: SDS Page, Expressing, Positron Emission Tomography, Sonication, Purification

    Growth of E. coli expressing BjHMA4R . BL21 transformants contained a pEASY-Blunt E1 expression vector (negative control) and BjHMA4R , respectively. The cultures were adjusted to an OD 600 of 1 and were serially diluted 10-fold in water. Five-microliter aliquots of each dilution were spotted either on nonselective LB plates or on LB plates supplemented with 600 μM CdCl 2 and 2.5 mM ZnCl 2, Ni(NO3) 2 , Co(NO3) 2 and Pb(NO3) 2 . After 1 day of incubation at 37 °C, the plates were imaged. The dilutions are indicated in the above figure, and three individual clones of each E. coli transformant were analyzed

    Journal: BMC Plant Biology

    Article Title: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

    doi: 10.1186/s12870-019-1674-5

    Figure Lengend Snippet: Growth of E. coli expressing BjHMA4R . BL21 transformants contained a pEASY-Blunt E1 expression vector (negative control) and BjHMA4R , respectively. The cultures were adjusted to an OD 600 of 1 and were serially diluted 10-fold in water. Five-microliter aliquots of each dilution were spotted either on nonselective LB plates or on LB plates supplemented with 600 μM CdCl 2 and 2.5 mM ZnCl 2, Ni(NO3) 2 , Co(NO3) 2 and Pb(NO3) 2 . After 1 day of incubation at 37 °C, the plates were imaged. The dilutions are indicated in the above figure, and three individual clones of each E. coli transformant were analyzed

    Article Snippet: The E. coli strain BL21 was part of a commercial batch from Transgen Biotech, China.

    Techniques: Expressing, Plasmid Preparation, Negative Control, Incubation, Clone Assay

    Dry weight of yeast and E. coli expressing BjHMA4R . a , b Yeast BY4741 and YK44 cells transformed with a pYES2 plasmid and a pYES2 plasmid that harbored BjHMA4R were grown in liquid YPD medium supplemented with 30 μM CdCl 2 . The cells were incubated at 30 °C for 10 h and 21 h. Fifteen milliliters of yeast fluid was collected by centrifugation and then dried at 95 °C for 48 h for determination of the dry weight. c , d , e E. coli BL21 cells transformed with a pEASY-Blunt E1 expression plasmid or with a pEASY-Blunt E1 expression plasmid that harbored BjHMA4R were grown in liquid LB medium supplemented with normal LB, 50 μM CdCl 2 and 100 μM CdCl 2 . The cells were incubated at 37 °C for 10 h and 20 h. Fifteen milliliters of E. coli fluid was collected by centrifugation and then dried at 95 °C for 48 h to determine the dry weight. The results are the means ± SEs of four independent experiments performed with four different colonies. Different letters above the columns indicate significant differences ( P

    Journal: BMC Plant Biology

    Article Title: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

    doi: 10.1186/s12870-019-1674-5

    Figure Lengend Snippet: Dry weight of yeast and E. coli expressing BjHMA4R . a , b Yeast BY4741 and YK44 cells transformed with a pYES2 plasmid and a pYES2 plasmid that harbored BjHMA4R were grown in liquid YPD medium supplemented with 30 μM CdCl 2 . The cells were incubated at 30 °C for 10 h and 21 h. Fifteen milliliters of yeast fluid was collected by centrifugation and then dried at 95 °C for 48 h for determination of the dry weight. c , d , e E. coli BL21 cells transformed with a pEASY-Blunt E1 expression plasmid or with a pEASY-Blunt E1 expression plasmid that harbored BjHMA4R were grown in liquid LB medium supplemented with normal LB, 50 μM CdCl 2 and 100 μM CdCl 2 . The cells were incubated at 37 °C for 10 h and 20 h. Fifteen milliliters of E. coli fluid was collected by centrifugation and then dried at 95 °C for 48 h to determine the dry weight. The results are the means ± SEs of four independent experiments performed with four different colonies. Different letters above the columns indicate significant differences ( P

    Article Snippet: The E. coli strain BL21 was part of a commercial batch from Transgen Biotech, China.

    Techniques: Expressing, Transformation Assay, Plasmid Preparation, Incubation, Centrifugation

    Cd and Zn contents of E. coli expressing BjHMA4R . E. coli BL21 cells transformed with a pEASY-Blunt E1 expression plasmid or a pEASY-Blunt E1 expression plasmid that harbored BjHMA4R were grown in normal liquid LB medium overnight, then supplemented with 50 μM CdCl 2 ( b ), 100 μM CdCl 2 ( c ), 200 μM ZnCl 2 ( e ) and normal LB ( a , d ). The cells were incubated at 37 °C for 10 h and 20 h. The metal contents of the samples were analyzed with inductively coupled plasma-mass spectrometry (ICP-MS). The results are the means ± SEs of four independent experiments performed with four different colonies. Different letters above the columns indicate significant differences ( P

    Journal: BMC Plant Biology

    Article Title: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

    doi: 10.1186/s12870-019-1674-5

    Figure Lengend Snippet: Cd and Zn contents of E. coli expressing BjHMA4R . E. coli BL21 cells transformed with a pEASY-Blunt E1 expression plasmid or a pEASY-Blunt E1 expression plasmid that harbored BjHMA4R were grown in normal liquid LB medium overnight, then supplemented with 50 μM CdCl 2 ( b ), 100 μM CdCl 2 ( c ), 200 μM ZnCl 2 ( e ) and normal LB ( a , d ). The cells were incubated at 37 °C for 10 h and 20 h. The metal contents of the samples were analyzed with inductively coupled plasma-mass spectrometry (ICP-MS). The results are the means ± SEs of four independent experiments performed with four different colonies. Different letters above the columns indicate significant differences ( P

    Article Snippet: The E. coli strain BL21 was part of a commercial batch from Transgen Biotech, China.

    Techniques: Expressing, Transformation Assay, Plasmid Preparation, Incubation, Mass Spectrometry