e coli bl21 (Stratagene)
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E Coli Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 173 article reviews
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1) Product Images from "Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification"
Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification
Journal: International Journal of Molecular Medicine
doi: 10.3892/ijmm.2012.890

Figure Legend Snippet: SDS-PAGE analysis of purified protein recombinant SllB in E. coli BL21 cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
Techniques Used: SDS Page, Purification, Recombinant, Marker

Figure Legend Snippet: A transmission electron microscopic observation on the E. coli BL21 with recombinant protein. (A) Normal E. coli BL21 was treated as control. (B) The E. coli BL21 cells recombinant S-layer protein. (C) Crystal lattice structures on surface of the E. coli BL21 cells recombinant S-layer protein.
Techniques Used: Transmission Assay, Recombinant

Figure Legend Snippet: Expression of sllB in E. coli BL21 cells. (A) SDS-PAGE analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; lane M represent Takara Protein Marker (Broad). (B) Western blot analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; M, Takara Protein Marker (Broad). Lane M1, Precision plus protein standards; lane M2, perfect protein marker. Note the band pointed with arrows is the recombinant S-layer protein.
Techniques Used: Expressing, SDS Page, Marker, Western Blot, Recombinant
2) Product Images from "Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation"
Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation
Journal: Molecular bioSystems
doi: 10.1039/c2mb25051a

Figure Legend Snippet: Abl kinase domain and Abl SH3-SH2-Kinase single chain multi-domain expression in bacteria and their purification analysed by SDS-PAGE and Western blot. (A) SDS-PAGE of Abl kinase: whole-cell lysate (lane 1); soluble proteins after centrifuge (lane 2); proteins bound on Co 2+ resin (lane3); retaining proteins on Co 2+ resin after thrombin cleavage (lane 4). (B) Tyrosine phosphorylation status: whole-cell lysate of BL21 with GroEL expression (lane 1); whole-cell lysate of BL21 with GroEL and Abl kinase domain co-expression (lane 2); proteins bound on Co 2+ resin (lane 3); proteins on Co 2+ resin after thrombin cleavage (lane 4); proteins eluted from the affinity resin (lane 5); protein from Lane 5 after CIP treatment (lane 6); dephosphorylated Abl kinase domain after purification (lane 7). The upper panel is Coomassie blue stained SDS-PAGE, the lower panel is an immunoblot with anti-phosphotyrosine antibody. (C) SDS-PAGE of Abl SH3-SH2-kinase: cell lysate before and after centrifuge (land 1 and 2); proteins remained on and eluted from the TALON resin after thrombin cleavage (land 3 and 4); after ionic exchange purification (lane 5); after both ionic exchange and size exclusion purification (lane 6); NMR sample before and after measurement (lane 7 and 8).
Techniques Used: Expressing, Purification, SDS Page, Western Blot, Staining, Nuclear Magnetic Resonance
3) Product Images from "Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen"
Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen
Journal: American Journal of Translational Research
doi:

Figure Legend Snippet: Expression and purification of recombinant protein Der f 21. A. SDS-PAGE analysis of the protein expressed from the pCold-TF- Der f 21 recombinant plasmid in E. coli BL21 cells. Lane M , TaKaRa Protein Marker (Broad); Lane 1 , the whole cell lysate of
Techniques Used: Expressing, Purification, Recombinant, SDS Page, Plasmid Preparation, Marker
4) Product Images from "Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification"
Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification
Journal: International Journal of Molecular Medicine
doi: 10.3892/ijmm.2012.890

Figure Legend Snippet: SDS-PAGE analysis of purified protein recombinant SllB in E. coli BL21 cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
Techniques Used: SDS Page, Purification, Recombinant, Marker

Figure Legend Snippet: A transmission electron microscopic observation on the E. coli BL21 with recombinant protein. (A) Normal E. coli BL21 was treated as control. (B) The E. coli BL21 cells recombinant S-layer protein. (C) Crystal lattice structures on surface of the E. coli BL21 cells recombinant S-layer protein.
Techniques Used: Transmission Assay, Recombinant

Figure Legend Snippet: Expression of sllB in E. coli BL21 cells. (A) SDS-PAGE analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; lane M represent Takara Protein Marker (Broad). (B) Western blot analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; M, Takara Protein Marker (Broad). Lane M1, Precision plus protein standards; lane M2, perfect protein marker. Note the band pointed with arrows is the recombinant S-layer protein.
Techniques Used: Expressing, SDS Page, Marker, Western Blot, Recombinant
5) Product Images from "Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes"
Article Title: Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes
Journal: Journal of Virology
doi: 10.1128/JVI.03274-13

Figure Legend Snippet: Effects of holin overproduction on burst size and lysis time of T7 phage infection. (A and B) The T7 phage burst size (A) and lysis time (B) were measured with infection of the BL21 host carrying a pET21 vector containing the holin gene under 1.5 mM IPTG-induced
Techniques Used: Lysis, Infection, Plasmid Preparation
6) Product Images from "Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae"
Article Title: Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae
Journal: Brazilian Journal of Medical and Biological Research
doi: 10.1590/S0100-879X2012007500077

Figure Legend Snippet: Expression of rDer f 5 in Escherichia coli BL21 cells. E. coli BL21 cells were transformed with either pET28a(+)-Der f 5 or empty vector pET28a(+) as control. A , SDS-PAGE analysis of the rDer f 5 protein. Lane M 1 = TaKaRa protein marker (Broad); lane 1 = whole cell lysate of E. coli BL21 cells containing pET28a; lane 2 = supernatant of cells containing pET28a; lane 3 = pellet of cells containing pET28a; lane 4 = whole cell lysate of E. coli BL21 cells containing pET28a(+)-Der f 5; lane 5 = supernatant of cells containing pET28a(+)-Der f 5; lane 6 = pellet of cells containing pET28a(+)-Der f 5. B , Western blotting analysis of the rDer f 5 protein. Lane M 2 = Precision Plus Protein Standards; lane 1 = whole cell lysate of E. coli BL21 cells containing pET28a; lane 2 = whole cell lysate of E. coli BL21 cells containing pET28a(+)-Der f 5. Arrows point to the band of rDer f 5.
Techniques Used: Expressing, Transformation Assay, Plasmid Preparation, SDS Page, Marker, Western Blot
7) Product Images from "Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen"
Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen
Journal: American Journal of Translational Research
doi:

Figure Legend Snippet: Expression and purification of recombinant protein Der f 21. A. SDS-PAGE analysis of the protein expressed from the pCold-TF- Der f 21 recombinant plasmid in E. coli BL21 cells. Lane M , TaKaRa Protein Marker (Broad); Lane 1 , the whole cell lysate of
Techniques Used: Expressing, Purification, Recombinant, SDS Page, Plasmid Preparation, Marker
8) Product Images from "Tetrachloroethene Dehalogenase from Dehalospirillum multivorans: Cloning, Sequencing of the Encoding Genes, and Expression of the pceA Gene in Escherichia coli"
Article Title: Tetrachloroethene Dehalogenase from Dehalospirillum multivorans: Cloning, Sequencing of the Encoding Genes, and Expression of the pceA Gene in Escherichia coli
Journal: Journal of Bacteriology
doi:

Figure Legend Snippet: Expression of pceA from D. multivorans in E. coli BL21 (DE3) as analyzed by SDS-PAGE. Strains of E. coli BL21 (DE3) harboring different plasmids were induced by IPTG. Cell extracts were analyzed by SDSâÂÂ12% PAGE and subsequently stained
Techniques Used: Expressing, SDS Page, Polyacrylamide Gel Electrophoresis, Staining
9) Product Images from "Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine"
Article Title: Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine
Journal: Scientific Reports
doi: 10.1038/srep39235

Figure Legend Snippet: HPTLC chromatogram of total lipid extracts from phage isolates. Lane 1: Escherichia coli BL21 positive control, lane 2: wild-type phage T7; lane 3: mutant phage T7::PhoE. Arrow head indicates the lipid species that is particularly enriched in the T7::PhoE mutant.
Techniques Used: High Performance Thin Layer Chromatography, Positive Control, Mutagenesis
10) Product Images from "Tetrachloroethene Dehalogenase from Dehalospirillum multivorans: Cloning, Sequencing of the Encoding Genes, and Expression of the pceA Gene in Escherichia coli"
Article Title: Tetrachloroethene Dehalogenase from Dehalospirillum multivorans: Cloning, Sequencing of the Encoding Genes, and Expression of the pceA Gene in Escherichia coli
Journal: Journal of Bacteriology
doi:

Figure Legend Snippet: Expression of pceA from D. multivorans in E. coli BL21 (DE3) as analyzed by SDS-PAGE. Strains of E. coli BL21 (DE3) harboring different plasmids were induced by IPTG. Cell extracts were analyzed by SDS–12% PAGE and subsequently stained
Techniques Used: Expressing, SDS Page, Polyacrylamide Gel Electrophoresis, Staining
11) Product Images from "Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification"
Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification
Journal: International Journal of Molecular Medicine
doi: 10.3892/ijmm.2012.890

Figure Legend Snippet: SDS-PAGE analysis of purified protein recombinant SllB in E. coli BL21 cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
Techniques Used: SDS Page, Purification, Recombinant, Marker

Figure Legend Snippet: A transmission electron microscopic observation on the E. coli BL21 with recombinant protein. (A) Normal E. coli BL21 was treated as control. (B) The E. coli BL21 cells recombinant S-layer protein. (C) Crystal lattice structures on surface of the E. coli BL21 cells recombinant S-layer protein.
Techniques Used: Transmission Assay, Recombinant

Figure Legend Snippet: Expression of sllB in E. coli BL21 cells. (A) SDS-PAGE analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; lane M represent Takara Protein Marker (Broad). (B) Western blot analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; M, Takara Protein Marker (Broad). Lane M1, Precision plus protein standards; lane M2, perfect protein marker. Note the band pointed with arrows is the recombinant S-layer protein.
Techniques Used: Expressing, SDS Page, Marker, Western Blot, Recombinant
12) Product Images from "Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes"
Article Title: Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes
Journal: Journal of Virology
doi: 10.1128/JVI.03274-13

Figure Legend Snippet: Effects of holin overproduction on burst size and lysis time of T7 phage infection. (A and B) The T7 phage burst size (A) and lysis time (B) were measured with infection of the BL21 host carrying a pET21 vector containing the holin gene under 1.5 mM IPTG-induced
Techniques Used: Lysis, Infection, Plasmid Preparation
13) Product Images from "Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes"
Article Title: Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes
Journal: Journal of Virology
doi: 10.1128/JVI.03274-13

Figure Legend Snippet: Effects of holin overproduction on burst size and lysis time of T7 phage infection. (A and B) The T7 phage burst size (A) and lysis time (B) were measured with infection of the BL21 host carrying a pET21 vector containing the holin gene under 1.5 mM IPTG-induced
Techniques Used: Lysis, Infection, Plasmid Preparation
14) Product Images from "Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine"
Article Title: Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine
Journal: Scientific Reports
doi: 10.1038/srep39235

Figure Legend Snippet: HPTLC chromatogram of total lipid extracts from phage isolates. Lane 1: Escherichia coli BL21 positive control, lane 2: wild-type phage T7; lane 3: mutant phage T7::PhoE. Arrow head indicates the lipid species that is particularly enriched in the T7::PhoE mutant.
Techniques Used: High Performance Thin Layer Chromatography, Positive Control, Mutagenesis
15) Product Images from "Tetrachloroethene Dehalogenase from Dehalospirillum multivorans: Cloning, Sequencing of the Encoding Genes, and Expression of the pceA Gene in Escherichia coli"
Article Title: Tetrachloroethene Dehalogenase from Dehalospirillum multivorans: Cloning, Sequencing of the Encoding Genes, and Expression of the pceA Gene in Escherichia coli
Journal: Journal of Bacteriology
doi:

Figure Legend Snippet: Expression of pceA from D. multivorans in E. coli BL21 (DE3) as analyzed by SDS-PAGE. Strains of E. coli BL21 (DE3) harboring different plasmids were induced by IPTG. Cell extracts were analyzed by SDS–12% PAGE and subsequently stained
Techniques Used: Expressing, SDS Page, Polyacrylamide Gel Electrophoresis, Staining
16) Product Images from "Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation"
Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation
Journal: Molecular bioSystems
doi: 10.1039/c2mb25051a

Figure Legend Snippet: Abl kinase domain and Abl SH3-SH2-Kinase single chain multi-domain expression in bacteria and their purification analysed by SDS-PAGE and Western blot. (A) SDS-PAGE of Abl kinase: whole-cell lysate (lane 1); soluble proteins after centrifuge (lane 2); proteins bound on Co 2+ resin (lane3); retaining proteins on Co 2+ resin after thrombin cleavage (lane 4). (B) Tyrosine phosphorylation status: whole-cell lysate of BL21 with GroEL expression (lane 1); whole-cell lysate of BL21 with GroEL and Abl kinase domain co-expression (lane 2); proteins bound on Co 2+ resin (lane 3); proteins on Co 2+ resin after thrombin cleavage (lane 4); proteins eluted from the affinity resin (lane 5); protein from Lane 5 after CIP treatment (lane 6); dephosphorylated Abl kinase domain after purification (lane 7). The upper panel is Coomassie blue stained SDS-PAGE, the lower panel is an immunoblot with anti-phosphotyrosine antibody. (C) SDS-PAGE of Abl SH3-SH2-kinase: cell lysate before and after centrifuge (land 1 and 2); proteins remained on and eluted from the TALON resin after thrombin cleavage (land 3 and 4); after ionic exchange purification (lane 5); after both ionic exchange and size exclusion purification (lane 6); NMR sample before and after measurement (lane 7 and 8).
Techniques Used: Expressing, Purification, SDS Page, Western Blot, Staining, Nuclear Magnetic Resonance
17) Product Images from "Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae"
Article Title: Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae
Journal: Brazilian Journal of Medical and Biological Research
doi: 10.1590/S0100-879X2012007500077

Figure Legend Snippet: Expression of rDer f 5 in Escherichia coli BL21 cells. E. coli BL21 cells were transformed with either pET28a(+)-Der f 5 or empty vector pET28a(+) as control. A , SDS-PAGE analysis of the rDer f 5 protein. Lane M 1 = TaKaRa protein marker (Broad); lane 1 = whole cell lysate of E. coli BL21 cells containing pET28a; lane 2 = supernatant of cells containing pET28a; lane 3 = pellet of cells containing pET28a; lane 4 = whole cell lysate of E. coli BL21 cells containing pET28a(+)-Der f 5; lane 5 = supernatant of cells containing pET28a(+)-Der f 5; lane 6 = pellet of cells containing pET28a(+)-Der f 5. B , Western blotting analysis of the rDer f 5 protein. Lane M 2 = Precision Plus Protein Standards; lane 1 = whole cell lysate of E. coli BL21 cells containing pET28a; lane 2 = whole cell lysate of E. coli BL21 cells containing pET28a(+)-Der f 5. Arrows point to the band of rDer f 5.
Techniques Used: Expressing, Transformation Assay, Plasmid Preparation, SDS Page, Marker, Western Blot
18) Product Images from "Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen"
Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen
Journal: American Journal of Translational Research
doi:

Figure Legend Snippet: Expression and purification of recombinant protein Der f 21. A. SDS-PAGE analysis of the protein expressed from the pCold-TF- Der f 21 recombinant plasmid in E. coli BL21 cells. Lane M , TaKaRa Protein Marker (Broad); Lane 1 , the whole cell lysate of
Techniques Used: Expressing, Purification, Recombinant, SDS Page, Plasmid Preparation, Marker
19) Product Images from "Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine"
Article Title: Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine
Journal: Scientific Reports
doi: 10.1038/srep39235

Figure Legend Snippet: HPTLC chromatogram of total lipid extracts from phage isolates. Lane 1: Escherichia coli BL21 positive control, lane 2: wild-type phage T7; lane 3: mutant phage T7::PhoE. Arrow head indicates the lipid species that is particularly enriched in the T7::PhoE mutant.
Techniques Used: High Performance Thin Layer Chromatography, Positive Control, Mutagenesis
20) Product Images from "Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae"
Article Title: Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae
Journal: Brazilian Journal of Medical and Biological Research
doi: 10.1590/S0100-879X2012007500077

Figure Legend Snippet: Expression of rDer f 5 in Escherichia coli BL21 cells. E. coli BL21 cells were transformed with either pET28a(+)-Der f 5 or empty vector pET28a(+) as control. A , SDS-PAGE analysis of the rDer f 5 protein. Lane M 1 = TaKaRa protein marker (Broad); lane 1 = whole cell lysate of E. coli BL21 cells containing pET28a; lane 2 = supernatant of cells containing pET28a; lane 3 = pellet of cells containing pET28a; lane 4 = whole cell lysate of E. coli BL21 cells containing pET28a(+)-Der f 5; lane 5 = supernatant of cells containing pET28a(+)-Der f 5; lane 6 = pellet of cells containing pET28a(+)-Der f 5. B , Western blotting analysis of the rDer f 5 protein. Lane M 2 = Precision Plus Protein Standards; lane 1 = whole cell lysate of E. coli BL21 cells containing pET28a; lane 2 = whole cell lysate of E. coli BL21 cells containing pET28a(+)-Der f 5. Arrows point to the band of rDer f 5.
Techniques Used: Expressing, Transformation Assay, Plasmid Preparation, SDS Page, Marker, Western Blot
21) Product Images from "Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen"
Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen
Journal: American Journal of Translational Research
doi:

Figure Legend Snippet: Expression and purification of recombinant protein Der f 21. A. SDS-PAGE analysis of the protein expressed from the pCold-TF- Der f 21 recombinant plasmid in E. coli BL21 cells. Lane M , TaKaRa Protein Marker (Broad); Lane 1 , the whole cell lysate of
Techniques Used: Expressing, Purification, Recombinant, SDS Page, Plasmid Preparation, Marker
Related Articles
Ligation:Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation Article Snippet: .. Plasmid containing SH3-SH2-kinase, pMAL/SH32(C2A)kin and plasmid containing kinase domain for ligation, pMAL/SspDnaAblCkinase were simply transformed into Infection:Article Title: Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes Article Snippet: .. Phage Preparation:Article Title: Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes Article Snippet: .. Expressing:Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification Article Snippet: .. Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation Article Snippet: .. Plasmid containing SH3-SH2-kinase, pMAL/SH32(C2A)kin and plasmid containing kinase domain for ligation, pMAL/SspDnaAblCkinase were simply transformed into Sequencing:Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen Article Snippet: .. After sequencing, the verified pCold-TF-Der f 21 plasmids were transformed into Transformation Assay:Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification Article Snippet: .. Article Title: Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae Article Snippet: .. Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen Article Snippet: .. Transformed Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen Article Snippet: .. After sequencing, the verified pCold-TF-Der f 21 plasmids were transformed into Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation Article Snippet: .. Plasmid containing SH3-SH2-kinase, pMAL/SH32(C2A)kin and plasmid containing kinase domain for ligation, pMAL/SspDnaAblCkinase were simply transformed into Recombinant:Article Title: Cloning, expression, and analysis of a cDNA coding for the Dermatophagoides farinae group 21 (Der f 21) allergen Article Snippet: .. Transformed Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation Article Snippet: .. Plasmid containing SH3-SH2-kinase, pMAL/SH32(C2A)kin and plasmid containing kinase domain for ligation, pMAL/SspDnaAblCkinase were simply transformed into Plasmid Preparation:Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification Article Snippet: .. Article Title: Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae Article Snippet: .. Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation Article Snippet: .. Plasmid containing SH3-SH2-kinase, pMAL/SH32(C2A)kin and plasmid containing kinase domain for ligation, pMAL/SspDnaAblCkinase were simply transformed into |