e coli bl21 (Stratagene)
96
Structured Review
Stratagene
e coli bl21
E Coli Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 96/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli bl21/product/Stratagene
Average 96 stars, based on 133 article reviews
Price from $9.99 to $1999.99
E Coli Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 96/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli bl21/product/Stratagene
Average 96 stars, based on 133 article reviews
Price from $9.99 to $1999.99
e coli bl21 - by Bioz Stars,
2020-01
96/100 stars
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Images
1) Product Images from "Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification"
Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification
Journal: International Journal of Molecular Medicine
doi: 10.3892/ijmm.2012.890
Figure Legend Snippet: SDS-PAGE analysis of purified protein recombinant SllB in E. coli BL21 cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
Techniques Used: SDS Page, Purification, Recombinant, Marker
Figure Legend Snippet: A transmission electron microscopic observation on the E. coli BL21 with recombinant protein. (A) Normal E. coli BL21 was treated as control. (B) The E. coli BL21 cells recombinant S-layer protein. (C) Crystal lattice structures on surface of the E. coli BL21 cells recombinant S-layer protein.
Techniques Used: Transmission Assay, Recombinant
Figure Legend Snippet: Expression of sllB in E. coli BL21 cells. (A) SDS-PAGE analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; lane M represent Takara Protein Marker (Broad). (B) Western blot analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; M, Takara Protein Marker (Broad). Lane M1, Precision plus protein standards; lane M2, perfect protein marker. Note the band pointed with arrows is the recombinant S-layer protein.
Techniques Used: Expressing, SDS Page, Marker, Western Blot, Recombinant
2) Product Images from "Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine"
Article Title: Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine
Journal: Scientific Reports
doi: 10.1038/srep39235
Figure Legend Snippet: HPTLC chromatogram of total lipid extracts from phage isolates. Lane 1: Escherichia coli BL21 positive control, lane 2: wild-type phage T7; lane 3: mutant phage T7::PhoE. Arrow head indicates the lipid species that is particularly enriched in the T7::PhoE mutant.
Techniques Used: High Performance Thin Layer Chromatography, Positive Control, Mutagenesis
3) Product Images from "Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes"
Article Title: Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes
Journal: Journal of Virology
doi: 10.1128/JVI.03274-13
Figure Legend Snippet: Effects of holin overproduction on burst size and lysis time of T7 phage infection. (A and B) The T7 phage burst size (A) and lysis time (B) were measured with infection of the BL21 host carrying a pET21 vector containing the holin gene under 1.5 mM IPTG-induced
Techniques Used: Lysis, Infection, Plasmid Preparation
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DV801A), from which 0.5 μl was used to transform 100 μl of Article Title: Unfurling of the band 4.1, ezrin, radixin, moesin (FERM) domain of the merlin tumor suppressor Article Snippet: The untagged merlin-1 tail domain (residues 503–595) was amplified and cloned into pET24b expression vector (Novagen) using the Nde I and Xho I restriction sites. .. Proteins were expressed in Article Title: The adaptor protein PID1 regulates receptor-dependent endocytosis of postprandial triglyceride-rich lipoproteins Article Snippet: .. 2.6 GST-pulldown experiments For purification of GST-fusion proteins, the GST expression vector (pGex-6P, GE Healthcare) as well as the Flag-expression vector pCMV-Tag2B, both containing the respective insert (murine PID1 ; wild type and mutated LRP1 ICD; see for structure and mutations of the intracellular LRP1 domain) were transformed into Article Title: Methods for Expression, Purification, and Characterization of PqqE, a Radical SAM Enzyme in the PQQ Biosynthetic Pathway Article Snippet: .. 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Selection:Article Title: Microbial Synthesis of Non-Natural Anthraquinone Glucosides Displaying Superior Antiproliferative Properties Article Snippet: Article Title: The evolution of substrate discrimination in macrolide antibiotic resistance enzymes Article Snippet: E. coli was routinely cultured on LB-Lennox (Bioshop) at 37 °C overnight, and either 100 µg mL−1 ampicillin or 50 µg mL−1 kanamycin was used for plasmid selection. .. Activation Assay:Article Title: Immunotherapy With the PreS-based Grass Pollen Allergy Vaccine BM32 Induces Antibody Responses Protecting Against Hepatitis B Infection Article Snippet: 2.1 Expression and Purification of Recombinant PreS, Synthesis of PreS Overlapping Peptides, Sequence Alignments The procedure of the expression and purification of a hexahistidine-tagged recombinant preS protein (preS1 + preS2; genotype A; subtype adw2, GenBank: AAT28735.1 ) in Phage Preparation:Article Title: Lysis Delay and Burst Shrinkage of Coliphage T7 by Deletion of Terminator Tφ Reversed by Deletion of Early Genes Article Snippet: .. Lysis:Article Title: Validation of an Immunohistochemistry Assay for Detection of CD155, the Poliovirus Receptor, in Malignant Gliomas Article Snippet: Individual CD155 expression constructs were transformed and expressed under control of the T7 promoter in Positron Emission Tomography:Article Title: Methods for Expression, Purification, and Characterization of PqqE, a Radical SAM Enzyme in the PQQ Biosynthetic Pathway Article Snippet: The pET cloning system uses a strong T7 promoter, facilitating a robust overexpression of the protein encoded by the cloned gene. .. Once the sequence is confirmed, the plasmid harboring the correct construct is cotransformed into Chick Chorioallantoic Membrane Assay:Article Title: Methods for Expression, Purification, and Characterization of PqqE, a Radical SAM Enzyme in the PQQ Biosynthetic Pathway Article Snippet: Once the sequence is confirmed, the plasmid harboring the correct construct is cotransformed into |
![Saturation binding of [ 3 H] CP-55,940 on BL21-107 membranes expressing CB2. The assay was performed in triplicate as described in Materials and Methods.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2253291/bin/2638fig3.gif.jpg)