Structured Review

Promega e coli bl21
Killing of E. coli <t>BL21</t> (DE3) containing plasmid pHp200 (■) or pET30b (⧫) with 100 μg of ampicillin per ml (A) or 100 μg of ciprofloxacin per ml (B). Cultures contained 0.01 mM IPTG.
E Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli bl21/product/Promega
Average 99 stars, based on 45 article reviews
Price from $9.99 to $1999.99
e coli bl21 - by Bioz Stars, 2020-08
99/100 stars

Images

1) Product Images from "Joint Tolerance to ?-Lactam and Fluoroquinolone Antibiotics in Escherichia coli Results from Overexpression of hipA"

Article Title: Joint Tolerance to ?-Lactam and Fluoroquinolone Antibiotics in Escherichia coli Results from Overexpression of hipA

Journal: Antimicrobial Agents and Chemotherapy

doi:

Killing of E. coli BL21 (DE3) containing plasmid pHp200 (■) or pET30b (⧫) with 100 μg of ampicillin per ml (A) or 100 μg of ciprofloxacin per ml (B). Cultures contained 0.01 mM IPTG.
Figure Legend Snippet: Killing of E. coli BL21 (DE3) containing plasmid pHp200 (■) or pET30b (⧫) with 100 μg of ampicillin per ml (A) or 100 μg of ciprofloxacin per ml (B). Cultures contained 0.01 mM IPTG.

Techniques Used: Plasmid Preparation

Growth curve of E. coli BL21 (DE3) carrying pLysS and pHp200 with and without IPTG induction. OD 600 , optical density at 600 nm.
Figure Legend Snippet: Growth curve of E. coli BL21 (DE3) carrying pLysS and pHp200 with and without IPTG induction. OD 600 , optical density at 600 nm.

Techniques Used:

2) Product Images from "A novel dimeric thymosin beta 4 with enhanced activities accelerates the rate of wound healing"

Article Title: A novel dimeric thymosin beta 4 with enhanced activities accelerates the rate of wound healing

Journal: Drug Design, Development and Therapy

doi: 10.2147/DDDT.S50183

Cloning, expression, and large-scale cultivation of dimeric thymosin beta 4 (DTβ4) engineered bacteria. ( A ) Two entire complementary DNA sequences of thymosin beta 4 (Tβ4) were constructed into a prokaryotic expression plasmid with a small DNA linker (GGTTCT). The results show a 267 bp fragment with correct sequence as expected (arrow). ( B ) Five colonies obtained after the transformation of Escherichia coli were randomly picked to test the protein expression on a small scale with sodium dodecyl sulfate polyacrylamide gel electrophoresis. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, a new protein (arrow) appeared in each culture pellet and accounted for over 15% of all the bacteria proteins (1: molecular ladder; 2–6: protein expression of picked five clones; 7: protein expression without IPTG induction). ( C ) Bacterial growth curve of BL21/DTβ4 in a 10 L fermenter (the arrow indicates the induction time). ( D ) DTβ4 expression (arrow) during fermentation (1: DTβ4 expression without IPTG induction; 2–6: DTβ4 expression every hour after IPTG induction). Abbreviation: DNA, deoxyribonucleic acid.
Figure Legend Snippet: Cloning, expression, and large-scale cultivation of dimeric thymosin beta 4 (DTβ4) engineered bacteria. ( A ) Two entire complementary DNA sequences of thymosin beta 4 (Tβ4) were constructed into a prokaryotic expression plasmid with a small DNA linker (GGTTCT). The results show a 267 bp fragment with correct sequence as expected (arrow). ( B ) Five colonies obtained after the transformation of Escherichia coli were randomly picked to test the protein expression on a small scale with sodium dodecyl sulfate polyacrylamide gel electrophoresis. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, a new protein (arrow) appeared in each culture pellet and accounted for over 15% of all the bacteria proteins (1: molecular ladder; 2–6: protein expression of picked five clones; 7: protein expression without IPTG induction). ( C ) Bacterial growth curve of BL21/DTβ4 in a 10 L fermenter (the arrow indicates the induction time). ( D ) DTβ4 expression (arrow) during fermentation (1: DTβ4 expression without IPTG induction; 2–6: DTβ4 expression every hour after IPTG induction). Abbreviation: DNA, deoxyribonucleic acid.

Techniques Used: Clone Assay, Expressing, Construct, Plasmid Preparation, Sequencing, Transformation Assay, Polyacrylamide Gel Electrophoresis

3) Product Images from "Immunization of mice by Hollow Mesoporous Silica Nanoparticles as carriers of Porcine Circovirus Type 2 ORF2 Protein"

Article Title: Immunization of mice by Hollow Mesoporous Silica Nanoparticles as carriers of Porcine Circovirus Type 2 ORF2 Protein

Journal: Virology Journal

doi: 10.1186/1743-422X-9-108

The expression of GST-ORF2-E protein. GST-ORF2-E protein was analyzed by (a) SDS-PAGE and (b) Western blot with an anti-GST monoclonal antibody. Lane 1: the third elution; Lane 2: the second elution; Lane 3: the first elution; Lane 4, supernatant of cell lysate after sonication; Lane 5: cell pellet after sonication; Lane 6: BL21 cells lysate after induction of IPTG; Lane 7, BL21 cells lysate before induction of IPTG. A clear band of 29 kDa was observed after induction.
Figure Legend Snippet: The expression of GST-ORF2-E protein. GST-ORF2-E protein was analyzed by (a) SDS-PAGE and (b) Western blot with an anti-GST monoclonal antibody. Lane 1: the third elution; Lane 2: the second elution; Lane 3: the first elution; Lane 4, supernatant of cell lysate after sonication; Lane 5: cell pellet after sonication; Lane 6: BL21 cells lysate after induction of IPTG; Lane 7, BL21 cells lysate before induction of IPTG. A clear band of 29 kDa was observed after induction.

Techniques Used: Expressing, SDS Page, Western Blot, Sonication

Related Articles

Pull Down Assay:

Article Title: C9-ALS/FTD-linked proline–arginine dipeptide repeat protein associates with paraspeckle components and increases paraspeckle formation
Article Snippet: .. Pull-down assay Recombinant proteins were produced using Escherichia coli BL21(DE3)pLysS competent cells (Promega, Madison, WI) as the host and the pGEX vector system (GE Healthcare UK Ltd). .. NSC-34 cells or NSC-34 cells, transiently transfected with indicated vectors for 48 h, were harvested and lysed in a pull-down buffer (150 mM NaCl, 20 mM HEPES [pH 7.4], 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and protease inhibitors) by sonication.

Ligation:

Article Title: Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans
Article Snippet: .. The ligation was therefore transformed into competent BL21(DE3)pLysS E. coli cells (Promega) expressing the pREP4 repressor plasmid. .. Transformed cells were grown in Terrific Broth (TB) medium with 100 µg ml−1 ampicillin, 35 µg ml−1 kanamycin and 12.5 µg ml−1 chloramphenicol.

Produced:

Article Title: C9-ALS/FTD-linked proline–arginine dipeptide repeat protein associates with paraspeckle components and increases paraspeckle formation
Article Snippet: .. Pull-down assay Recombinant proteins were produced using Escherichia coli BL21(DE3)pLysS competent cells (Promega, Madison, WI) as the host and the pGEX vector system (GE Healthcare UK Ltd). .. NSC-34 cells or NSC-34 cells, transiently transfected with indicated vectors for 48 h, were harvested and lysed in a pull-down buffer (150 mM NaCl, 20 mM HEPES [pH 7.4], 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and protease inhibitors) by sonication.

Article Title: Immunogenic and protective properties of GP5 and M structural proteins of porcine reproductive and respiratory syndrome virus expressed from replicating but nondisseminating adenovectors
Article Snippet: .. Recombinant GP5 in fusion with Glutathione S-transferase (GST), designated hereafter rGP5, was produced in BL21 (DE3)pLysS competent E . coli cells (Promega, Madison, WI, USA) upon induction at OD(600 nm) of 1.2 with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 h at 37°C. .. Bacterial cells were lysed by sonication in buffer (PBS, 0.5% Tween-20, 0.5% triton X-100, 0.5% NP40) and the whole bacteria protein extract was separated through 12% SDS-PAGE.

other:

Article Title: Production and use of recombinant Aβ for aggregation studies
Article Snippet: Note 2: Preparation of chemically competent BL21* DE3 pLysS E. coli cells: add 1 μl of stock BL21* DE3 pLysS cells (Promega Cat. #L1195) to 50 ml of autoclaved LB media containing 38 μg/ml of chloramphenicol.

Expressing:

Article Title: Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans
Article Snippet: .. The ligation was therefore transformed into competent BL21(DE3)pLysS E. coli cells (Promega) expressing the pREP4 repressor plasmid. .. Transformed cells were grown in Terrific Broth (TB) medium with 100 µg ml−1 ampicillin, 35 µg ml−1 kanamycin and 12.5 µg ml−1 chloramphenicol.

Article Title: High-throughput small RNA sequencing enhanced by AlkB-facilitated RNA de-Methylation (ARM-seq)
Article Snippet: .. E. coli BL21(DE3)pLysS competent cells (Promega) were transformed with AlkB-AVA421 plasmid according to manufacturer’s protocol (any equivalent E. coli competent cells commonly used for protein expression may be used). .. Frozen cell pellet from 12 L culture of AlkB-AVA421-transformed cells grown at 37°C to OD600 0.5 and induced with IPTG for 2 h. Sonication buffer: 20 mM Hepes pH 7.5, 5% glycerol, 1 M NaCl, 2 mM β-mercaptoethanol (BME), 2 μg/mL Leupeptin, 1 μg/mL Pepstatin, 1 mM Pefabloc TALON Metal Affinity Resin, 2 mL per liter of bacterial culture.

Transformation Assay:

Article Title: Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans
Article Snippet: .. The ligation was therefore transformed into competent BL21(DE3)pLysS E. coli cells (Promega) expressing the pREP4 repressor plasmid. .. Transformed cells were grown in Terrific Broth (TB) medium with 100 µg ml−1 ampicillin, 35 µg ml−1 kanamycin and 12.5 µg ml−1 chloramphenicol.

Article Title: High-throughput small RNA sequencing enhanced by AlkB-facilitated RNA de-Methylation (ARM-seq)
Article Snippet: .. E. coli BL21(DE3)pLysS competent cells (Promega) were transformed with AlkB-AVA421 plasmid according to manufacturer’s protocol (any equivalent E. coli competent cells commonly used for protein expression may be used). .. Frozen cell pellet from 12 L culture of AlkB-AVA421-transformed cells grown at 37°C to OD600 0.5 and induced with IPTG for 2 h. Sonication buffer: 20 mM Hepes pH 7.5, 5% glycerol, 1 M NaCl, 2 mM β-mercaptoethanol (BME), 2 μg/mL Leupeptin, 1 μg/mL Pepstatin, 1 mM Pefabloc TALON Metal Affinity Resin, 2 mL per liter of bacterial culture.

Article Title: The NarE protein of Neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion
Article Snippet: .. Escherichia coli BL21 (DE3) pLysS competent cells (Promega) were transformed with recombinant vectors and selected onto LB plates +kan+cam. .. Bacterial cultures were induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (Invitrogen) at OD600 of 0.5–0.6, grown for 4 h at 25°C with gentle shaking and harvested by centrifugation.

Article Title: The Oncogenic Small Tumor Antigen of Merkel Cell Polyomavirus Is an Iron-Sulfur Cluster Protein That Enhances Viral DNA Replication
Article Snippet: .. BL21(DE3)pLysS competent cells (Promega) were transformed with plasmids as indicated in the figure legends. .. Bacterial cultures were grown in 2×YT medium to an A 600 of ∼0.6 before 0.4 mM IPTG (isopropyl-β- d -thiogalactopyranoside) induction at 37°C for 4 h. Bacteria were collected, and sT purification was performed using solutions that were degassed and purged with oxygen-free nitrogen.

Recombinant:

Article Title: C9-ALS/FTD-linked proline–arginine dipeptide repeat protein associates with paraspeckle components and increases paraspeckle formation
Article Snippet: .. Pull-down assay Recombinant proteins were produced using Escherichia coli BL21(DE3)pLysS competent cells (Promega, Madison, WI) as the host and the pGEX vector system (GE Healthcare UK Ltd). .. NSC-34 cells or NSC-34 cells, transiently transfected with indicated vectors for 48 h, were harvested and lysed in a pull-down buffer (150 mM NaCl, 20 mM HEPES [pH 7.4], 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and protease inhibitors) by sonication.

Article Title: The NarE protein of Neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion
Article Snippet: .. Escherichia coli BL21 (DE3) pLysS competent cells (Promega) were transformed with recombinant vectors and selected onto LB plates +kan+cam. .. Bacterial cultures were induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (Invitrogen) at OD600 of 0.5–0.6, grown for 4 h at 25°C with gentle shaking and harvested by centrifugation.

Article Title: Immunogenic and protective properties of GP5 and M structural proteins of porcine reproductive and respiratory syndrome virus expressed from replicating but nondisseminating adenovectors
Article Snippet: .. Recombinant GP5 in fusion with Glutathione S-transferase (GST), designated hereafter rGP5, was produced in BL21 (DE3)pLysS competent E . coli cells (Promega, Madison, WI, USA) upon induction at OD(600 nm) of 1.2 with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 h at 37°C. .. Bacterial cells were lysed by sonication in buffer (PBS, 0.5% Tween-20, 0.5% triton X-100, 0.5% NP40) and the whole bacteria protein extract was separated through 12% SDS-PAGE.

Plasmid Preparation:

Article Title: Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans
Article Snippet: .. The ligation was therefore transformed into competent BL21(DE3)pLysS E. coli cells (Promega) expressing the pREP4 repressor plasmid. .. Transformed cells were grown in Terrific Broth (TB) medium with 100 µg ml−1 ampicillin, 35 µg ml−1 kanamycin and 12.5 µg ml−1 chloramphenicol.

Article Title: High-throughput small RNA sequencing enhanced by AlkB-facilitated RNA de-Methylation (ARM-seq)
Article Snippet: .. E. coli BL21(DE3)pLysS competent cells (Promega) were transformed with AlkB-AVA421 plasmid according to manufacturer’s protocol (any equivalent E. coli competent cells commonly used for protein expression may be used). .. Frozen cell pellet from 12 L culture of AlkB-AVA421-transformed cells grown at 37°C to OD600 0.5 and induced with IPTG for 2 h. Sonication buffer: 20 mM Hepes pH 7.5, 5% glycerol, 1 M NaCl, 2 mM β-mercaptoethanol (BME), 2 μg/mL Leupeptin, 1 μg/mL Pepstatin, 1 mM Pefabloc TALON Metal Affinity Resin, 2 mL per liter of bacterial culture.

Article Title: C9-ALS/FTD-linked proline–arginine dipeptide repeat protein associates with paraspeckle components and increases paraspeckle formation
Article Snippet: .. Pull-down assay Recombinant proteins were produced using Escherichia coli BL21(DE3)pLysS competent cells (Promega, Madison, WI) as the host and the pGEX vector system (GE Healthcare UK Ltd). .. NSC-34 cells or NSC-34 cells, transiently transfected with indicated vectors for 48 h, were harvested and lysed in a pull-down buffer (150 mM NaCl, 20 mM HEPES [pH 7.4], 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and protease inhibitors) by sonication.

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  • 92
    Promega e coli bl21 cells
    Expression of recombinant gametocyte protein EnGAM22. (A) Analysis of the expressed protein by SDS-PAGE. Protein marker (lane 1), the recombinant bacterial <t>BL21</t> containing recombinant expression vector induced by 1 mM IPTG (lane 2), the recombinant bacterial BL21 not induced by IPTG (lane3), the bacterial containing the wild type vector induced by IPTG (lane 4) and the bacterial BL21 induced by IPTG (lane 5). (B) The solubility analysis of recombinant protein. Protein marker (lane 1), supernatant of bacterial sonicates (lane 2) and sediments of bacterial sonicates (lane 3).
    E Coli Bl21 Cells, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 cells/product/Promega
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 cells - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Promega e coli bl21
    Killing of E. coli <t>BL21</t> (DE3) containing plasmid pHp200 (■) or pET30b (⧫) with 100 μg of ampicillin per ml (A) or 100 μg of ciprofloxacin per ml (B). Cultures contained 0.01 mM IPTG.
    E Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21/product/Promega
    Average 93 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expression of recombinant gametocyte protein EnGAM22. (A) Analysis of the expressed protein by SDS-PAGE. Protein marker (lane 1), the recombinant bacterial BL21 containing recombinant expression vector induced by 1 mM IPTG (lane 2), the recombinant bacterial BL21 not induced by IPTG (lane3), the bacterial containing the wild type vector induced by IPTG (lane 4) and the bacterial BL21 induced by IPTG (lane 5). (B) The solubility analysis of recombinant protein. Protein marker (lane 1), supernatant of bacterial sonicates (lane 2) and sediments of bacterial sonicates (lane 3).

    Journal: Parasites & Vectors

    Article Title: Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation

    doi: 10.1186/1756-3305-7-27

    Figure Lengend Snippet: Expression of recombinant gametocyte protein EnGAM22. (A) Analysis of the expressed protein by SDS-PAGE. Protein marker (lane 1), the recombinant bacterial BL21 containing recombinant expression vector induced by 1 mM IPTG (lane 2), the recombinant bacterial BL21 not induced by IPTG (lane3), the bacterial containing the wild type vector induced by IPTG (lane 4) and the bacterial BL21 induced by IPTG (lane 5). (B) The solubility analysis of recombinant protein. Protein marker (lane 1), supernatant of bacterial sonicates (lane 2) and sediments of bacterial sonicates (lane 3).

    Article Snippet: Recombinant protein expression from E. coli BL21 cells grown in lysogeny broth (LB) medium containing 30 μg/mL kanamycin at 37°C was induced using 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Promega Corp.) at an absorbance at 600 nm of 0.6.

    Techniques: Expressing, Recombinant, SDS Page, Marker, Plasmid Preparation, Solubility

    Killing of E. coli BL21 (DE3) containing plasmid pHp200 (■) or pET30b (⧫) with 100 μg of ampicillin per ml (A) or 100 μg of ciprofloxacin per ml (B). Cultures contained 0.01 mM IPTG.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Joint Tolerance to ?-Lactam and Fluoroquinolone Antibiotics in Escherichia coli Results from Overexpression of hipA

    doi:

    Figure Lengend Snippet: Killing of E. coli BL21 (DE3) containing plasmid pHp200 (■) or pET30b (⧫) with 100 μg of ampicillin per ml (A) or 100 μg of ciprofloxacin per ml (B). Cultures contained 0.01 mM IPTG.

    Article Snippet: Growth of E. coli BL21 (DE3) carrying pLysS (Promega) and pHp200, a pET30b (Novagen) construct containing the entire hipA open reading frame, was induced with 0.05 to 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG).

    Techniques: Plasmid Preparation

    Growth curve of E. coli BL21 (DE3) carrying pLysS and pHp200 with and without IPTG induction. OD 600 , optical density at 600 nm.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Joint Tolerance to ?-Lactam and Fluoroquinolone Antibiotics in Escherichia coli Results from Overexpression of hipA

    doi:

    Figure Lengend Snippet: Growth curve of E. coli BL21 (DE3) carrying pLysS and pHp200 with and without IPTG induction. OD 600 , optical density at 600 nm.

    Article Snippet: Growth of E. coli BL21 (DE3) carrying pLysS (Promega) and pHp200, a pET30b (Novagen) construct containing the entire hipA open reading frame, was induced with 0.05 to 1 mM isopropyl-β- d -thiogalactopyranoside (IPTG).

    Techniques:

    Cloning, expression, and large-scale cultivation of dimeric thymosin beta 4 (DTβ4) engineered bacteria. ( A ) Two entire complementary DNA sequences of thymosin beta 4 (Tβ4) were constructed into a prokaryotic expression plasmid with a small DNA linker (GGTTCT). The results show a 267 bp fragment with correct sequence as expected (arrow). ( B ) Five colonies obtained after the transformation of Escherichia coli were randomly picked to test the protein expression on a small scale with sodium dodecyl sulfate polyacrylamide gel electrophoresis. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, a new protein (arrow) appeared in each culture pellet and accounted for over 15% of all the bacteria proteins (1: molecular ladder; 2–6: protein expression of picked five clones; 7: protein expression without IPTG induction). ( C ) Bacterial growth curve of BL21/DTβ4 in a 10 L fermenter (the arrow indicates the induction time). ( D ) DTβ4 expression (arrow) during fermentation (1: DTβ4 expression without IPTG induction; 2–6: DTβ4 expression every hour after IPTG induction). Abbreviation: DNA, deoxyribonucleic acid.

    Journal: Drug Design, Development and Therapy

    Article Title: A novel dimeric thymosin beta 4 with enhanced activities accelerates the rate of wound healing

    doi: 10.2147/DDDT.S50183

    Figure Lengend Snippet: Cloning, expression, and large-scale cultivation of dimeric thymosin beta 4 (DTβ4) engineered bacteria. ( A ) Two entire complementary DNA sequences of thymosin beta 4 (Tβ4) were constructed into a prokaryotic expression plasmid with a small DNA linker (GGTTCT). The results show a 267 bp fragment with correct sequence as expected (arrow). ( B ) Five colonies obtained after the transformation of Escherichia coli were randomly picked to test the protein expression on a small scale with sodium dodecyl sulfate polyacrylamide gel electrophoresis. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, a new protein (arrow) appeared in each culture pellet and accounted for over 15% of all the bacteria proteins (1: molecular ladder; 2–6: protein expression of picked five clones; 7: protein expression without IPTG induction). ( C ) Bacterial growth curve of BL21/DTβ4 in a 10 L fermenter (the arrow indicates the induction time). ( D ) DTβ4 expression (arrow) during fermentation (1: DTβ4 expression without IPTG induction; 2–6: DTβ4 expression every hour after IPTG induction). Abbreviation: DNA, deoxyribonucleic acid.

    Article Snippet: Expression of DTβ4 and large-scale cultivation of engineered bacteria The pET22b-DTβ4 plasmid was transformed into E. coli BL21 (DE3; Promega) using the calcium chloride method.

    Techniques: Clone Assay, Expressing, Construct, Plasmid Preparation, Sequencing, Transformation Assay, Polyacrylamide Gel Electrophoresis

    The expression of GST-ORF2-E protein. GST-ORF2-E protein was analyzed by (a) SDS-PAGE and (b) Western blot with an anti-GST monoclonal antibody. Lane 1: the third elution; Lane 2: the second elution; Lane 3: the first elution; Lane 4, supernatant of cell lysate after sonication; Lane 5: cell pellet after sonication; Lane 6: BL21 cells lysate after induction of IPTG; Lane 7, BL21 cells lysate before induction of IPTG. A clear band of 29 kDa was observed after induction.

    Journal: Virology Journal

    Article Title: Immunization of mice by Hollow Mesoporous Silica Nanoparticles as carriers of Porcine Circovirus Type 2 ORF2 Protein

    doi: 10.1186/1743-422X-9-108

    Figure Lengend Snippet: The expression of GST-ORF2-E protein. GST-ORF2-E protein was analyzed by (a) SDS-PAGE and (b) Western blot with an anti-GST monoclonal antibody. Lane 1: the third elution; Lane 2: the second elution; Lane 3: the first elution; Lane 4, supernatant of cell lysate after sonication; Lane 5: cell pellet after sonication; Lane 6: BL21 cells lysate after induction of IPTG; Lane 7, BL21 cells lysate before induction of IPTG. A clear band of 29 kDa was observed after induction.

    Article Snippet: Expression of protein PCV2 ORF2-E protein was expressed in E. coli BL21 as described previously [ ] The GST-ORF2-E fusion protein was purified by a MagneGST™ Protein Purification System (Promega, USA).

    Techniques: Expressing, SDS Page, Western Blot, Sonication