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Stratagene e coli bl21 de3 ripl cells
A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli <t>BL21</t> (DE3) <t>RIPL</t> strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells
E Coli Bl21 De3 Ripl Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine"

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine

Journal: Journal of molecular biology

doi: 10.1016/j.jmb.2013.10.011

A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli BL21 (DE3) RIPL strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells
Figure Legend Snippet: A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli BL21 (DE3) RIPL strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells

Techniques Used: Transformation Assay, Expressing

2) Product Images from "Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine"

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine

Journal: Journal of molecular biology

doi: 10.1016/j.jmb.2013.10.011

A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli BL21 (DE3) RIPL strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells
Figure Legend Snippet: A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli BL21 (DE3) RIPL strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells

Techniques Used: Transformation Assay, Expressing

Related Articles

Clone Assay:

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine
Article Snippet: .. The plasmid clones were then transformed into E. coli BL21 (DE3) RIPL cells (Stratagene) for expression of recombinant proteins. .. E. coli P301 was used as a suppressor-negative strain and E. coli B40 ( sup ) was used for preparation of T4 phage amber mutant stocks.

Cell Culture:

Article Title: Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for aspartate A (CcaA)
Article Snippet: .. E. coli BL21(DE3)-RIPL cells (Stratagene) were transformed with the expression vector and cultured to exponential phase at 37°C in LB medium containing 50 µg ml−1 ampicillin. .. Expression was induced with 1 m M isopropyl β- d -1-thiogalactopyranoside at an OD600 nm of 0.6.

Purification:

Article Title: Site-Specific Monoubiquitination Activates Ras by Impeding GTPase Activating Protein Function
Article Snippet: .. Protein Purification The Ras domains (1–166) of H–Ras and K–Ras were expressed in the pQlinkH vector (Addgene) with a histidine purification tag in Escherichia coli BL21 (DE3) RIPL cells (Stratagene: La Jolla, CA). .. Proteins were purified following standard Qiagen nickel affinity purification procedures.

Protein Purification:

Article Title: Site-Specific Monoubiquitination Activates Ras by Impeding GTPase Activating Protein Function
Article Snippet: .. Protein Purification The Ras domains (1–166) of H–Ras and K–Ras were expressed in the pQlinkH vector (Addgene) with a histidine purification tag in Escherichia coli BL21 (DE3) RIPL cells (Stratagene: La Jolla, CA). .. Proteins were purified following standard Qiagen nickel affinity purification procedures.

Expressing:

Article Title: Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for aspartate A (CcaA)
Article Snippet: .. E. coli BL21(DE3)-RIPL cells (Stratagene) were transformed with the expression vector and cultured to exponential phase at 37°C in LB medium containing 50 µg ml−1 ampicillin. .. Expression was induced with 1 m M isopropyl β- d -1-thiogalactopyranoside at an OD600 nm of 0.6.

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine
Article Snippet: .. The plasmid clones were then transformed into E. coli BL21 (DE3) RIPL cells (Stratagene) for expression of recombinant proteins. .. E. coli P301 was used as a suppressor-negative strain and E. coli B40 ( sup ) was used for preparation of T4 phage amber mutant stocks.

Transformation Assay:

Article Title: Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for aspartate A (CcaA)
Article Snippet: .. E. coli BL21(DE3)-RIPL cells (Stratagene) were transformed with the expression vector and cultured to exponential phase at 37°C in LB medium containing 50 µg ml−1 ampicillin. .. Expression was induced with 1 m M isopropyl β- d -1-thiogalactopyranoside at an OD600 nm of 0.6.

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine
Article Snippet: .. The plasmid clones were then transformed into E. coli BL21 (DE3) RIPL cells (Stratagene) for expression of recombinant proteins. .. E. coli P301 was used as a suppressor-negative strain and E. coli B40 ( sup ) was used for preparation of T4 phage amber mutant stocks.

Recombinant:

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine
Article Snippet: .. The plasmid clones were then transformed into E. coli BL21 (DE3) RIPL cells (Stratagene) for expression of recombinant proteins. .. E. coli P301 was used as a suppressor-negative strain and E. coli B40 ( sup ) was used for preparation of T4 phage amber mutant stocks.

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine
Article Snippet: .. E. coli BL21 (DE3) RIPL cells containing the gp20 recombinant plasmids was induced with isopropyl-β-D-thio-galactoside (IPTG; 1 mM) for 20 min at 37°C. .. The cells were infected with the 10am13am20am or 20am mutant phage [multiplicity of infection (MOI) = 4) and superinfected at the same MOI 7 min post-initial infection and incubated in a 37°C shaker for 28 min.

Article Title: Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for aspartate A (CcaA)
Article Snippet: .. Recombinant CcaAperi from C. jejuni was overexpressed in E. coli BL21(DE3)-RIPL cells. .. The purified protein fragment contained CcaA residues 31–327 plus six additional residues at the N-terminus (a cloning artifact; GIDPFT).

Plasmid Preparation:

Article Title: Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for aspartate A (CcaA)
Article Snippet: .. E. coli BL21(DE3)-RIPL cells (Stratagene) were transformed with the expression vector and cultured to exponential phase at 37°C in LB medium containing 50 µg ml−1 ampicillin. .. Expression was induced with 1 m M isopropyl β- d -1-thiogalactopyranoside at an OD600 nm of 0.6.

Article Title: Site-Specific Monoubiquitination Activates Ras by Impeding GTPase Activating Protein Function
Article Snippet: .. Protein Purification The Ras domains (1–166) of H–Ras and K–Ras were expressed in the pQlinkH vector (Addgene) with a histidine purification tag in Escherichia coli BL21 (DE3) RIPL cells (Stratagene: La Jolla, CA). .. Proteins were purified following standard Qiagen nickel affinity purification procedures.

Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine
Article Snippet: .. The plasmid clones were then transformed into E. coli BL21 (DE3) RIPL cells (Stratagene) for expression of recombinant proteins. .. E. coli P301 was used as a suppressor-negative strain and E. coli B40 ( sup ) was used for preparation of T4 phage amber mutant stocks.

Article Title: MLL protects CpG clusters from methylation within the Hoxa9 gene, maintaining transcript expression
Article Snippet: .. The plasmid was used to transform E. coli BL21 (DE3) RIPL cells (Stratagene). .. Protein expression was induced by addition of 1 mM IPTG.

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    Stratagene e coli bl21 de3 ripl cells
    A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli <t>BL21</t> (DE3) <t>RIPL</t> strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells
    E Coli Bl21 De3 Ripl Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 de3 ripl cells/product/Stratagene
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 de3 ripl cells - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    85
    Stratagene escherichia coli codon plus ripl bl21 cells
    A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli <t>BL21</t> (DE3) <t>RIPL</t> strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells
    Escherichia Coli Codon Plus Ripl Bl21 Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli codon plus ripl bl21 cells/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli codon plus ripl bl21 cells - by Bioz Stars, 2020-09
    85/100 stars
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    85
    Stratagene e coli bl21 codonplus ripl cells
    A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli <t>BL21</t> (DE3) <t>RIPL</t> strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells
    E Coli Bl21 Codonplus Ripl Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 codonplus ripl cells/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 codonplus ripl cells - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli BL21 (DE3) RIPL strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells

    Journal: Journal of molecular biology

    Article Title: Structure-Function Analysis of the DNA Translocating Portal of the Bacteriophage T4 Packaging Machine

    doi: 10.1016/j.jmb.2013.10.011

    Figure Lengend Snippet: A biochemical approach to dissect portal function. (a) The plasmids containing g20 variants (WT or mutants) were transformed into E. coli BL21 (DE3) RIPL strain for IPTG induced expression of the respective gp20 protein (green). (b) The E. coli cells

    Article Snippet: E. coli BL21 (DE3) RIPL cells containing the gp20 recombinant plasmids was induced with isopropyl-β-D-thio-galactoside (IPTG; 1 mM) for 20 min at 37°C.

    Techniques: Transformation Assay, Expressing