e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922
    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, <t>E.</t> <t>coli</t> ATCC 25922.
    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli atcc 25922 - by Bioz Stars, 2024-06
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    Images

    1) Product Images from "Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target"

    Article Title: Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target

    Journal: Nature Communications

    doi: 10.1038/s41467-024-49484-7

    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.
    Figure Legend Snippet: a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.

    Techniques Used: Activity Assay, Concentration Assay

    MraY inhibitory and antibacterial activity against ESKAPE pathogens
    Figure Legend Snippet: MraY inhibitory and antibacterial activity against ESKAPE pathogens

    Techniques Used: Activity Assay

    Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.
    Figure Legend Snippet: Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.

    Techniques Used: Analogues, Infection

    e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922
    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, <t>E.</t> <t>coli</t> ATCC 25922.
    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli atcc 25922/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli atcc 25922 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target"

    Article Title: Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target

    Journal: Nature Communications

    doi: 10.1038/s41467-024-49484-7

    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.
    Figure Legend Snippet: a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.

    Techniques Used: Activity Assay, Concentration Assay

    MraY inhibitory and antibacterial activity against ESKAPE pathogens
    Figure Legend Snippet: MraY inhibitory and antibacterial activity against ESKAPE pathogens

    Techniques Used: Activity Assay

    Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.
    Figure Legend Snippet: Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.

    Techniques Used: Analogues, Infection

    e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922
    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, <t>E.</t> <t>coli</t> ATCC 25922.
    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli atcc 25922/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli atcc 25922 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target"

    Article Title: Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target

    Journal: Nature Communications

    doi: 10.1038/s41467-024-49484-7

    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.
    Figure Legend Snippet: a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.

    Techniques Used: Activity Assay, Concentration Assay

    MraY inhibitory and antibacterial activity against ESKAPE pathogens
    Figure Legend Snippet: MraY inhibitory and antibacterial activity against ESKAPE pathogens

    Techniques Used: Activity Assay

    Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.
    Figure Legend Snippet: Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.

    Techniques Used: Analogues, Infection

    e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922

    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli atcc 25922/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli atcc 25922 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?"

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    Journal: bioRxiv

    doi: 10.1101/2024.06.13.596383


    Figure Legend Snippet:

    Techniques Used: Inhibition

    Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).

    Techniques Used: Concentration Assay, Bacteria, Cell Culture

    Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).
    Figure Legend Snippet: Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).

    Techniques Used: Inhibition

    Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.
    Figure Legend Snippet: Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.

    Techniques Used: Confocal Laser Scanning Microscopy, Bacteria, Staining

    e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922

    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli atcc 25922/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli atcc 25922 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?"

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    Journal: bioRxiv

    doi: 10.1101/2024.06.13.596383


    Figure Legend Snippet:

    Techniques Used: Inhibition

    Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).

    Techniques Used: Concentration Assay, Bacteria, Cell Culture

    Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).
    Figure Legend Snippet: Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).

    Techniques Used: Inhibition

    Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.
    Figure Legend Snippet: Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.

    Techniques Used: Confocal Laser Scanning Microscopy, Bacteria, Staining

    e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922

    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?"

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    Journal: bioRxiv

    doi: 10.1101/2024.06.13.596383


    Figure Legend Snippet:

    Techniques Used: Inhibition

    Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).

    Techniques Used: Concentration Assay, Bacteria, Cell Culture

    Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).
    Figure Legend Snippet: Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).

    Techniques Used: Inhibition

    Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.
    Figure Legend Snippet: Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.

    Techniques Used: Confocal Laser Scanning Microscopy, Bacteria, Staining

    e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922

    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?"

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    Journal: bioRxiv

    doi: 10.1101/2024.06.13.596383


    Figure Legend Snippet:

    Techniques Used: Inhibition

    Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNO 3 and silver-chitosan nanocomposites (nAgCS-1 and nAgCS-3) to Escherichia coli and Staphylococcus aureus after 24-h growth in CA-MHB50 at 37°C. The bacteria were continuously exposed to AgNO 3 (0.8 mg Ag/L for E. coli and 1.6 mg Ag/L for S. aureus ), nAgCS-1 (6.0 mg Ag/L) or nAgCS-3 (3.0 mg Ag/L) for 0–5 weeks in successive 24-hour culture steps. The control bacteria, not exposed to silver compounds, were sub-cultured under the same test conditions. MIC and MBC were determined weekly using the antibacterial susceptibility test (ISO 20776-1:2019). Asterisks designate to statistical differences (p ≤0.05).

    Techniques Used: Concentration Assay, Bacteria, Cell Culture

    Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).
    Figure Legend Snippet: Susceptibility (formation of growth-inhibition zone, mm) of Escherichia coli (A) and Staphylococcus aureus (B) after 0–5 weeks of continuous exposure (or not; control) to AgNO 3 and Ag-chitosan nanocomposites with weight ratios of 1:1 (nAgCS-1) and 1:3 (nAgCS-3) to the antibiotics (µg/disk): aztreonam (30), cefotaxime (5), ceftazidime (10), ampicillin (10), meropenem (10), cefoxitin (30), ciprofloxacin (5), chloramphenicol (30), gentamicin (10), tetracycline (30), clindamycin (2), erythromycin (15), oxacillin (1) and vancomycin (5).

    Techniques Used: Inhibition

    Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.
    Figure Legend Snippet: Confocal laser scanning microscopy images of Escherichia coli . The bacteria had been exposed (or not; control culture) to AgNO 3 (0.8 mg Ag/L) and silver-chitosan nanocomposites (nAgCSs) with weight ratios of 1:1 (nAgCS-1, 6.0 mg Ag/L) or 1:3 (nAgCS-3, 3.0 mg Ag/L) for 0–5 weeks in consecutive 24-hour sub-cultures in 50% Mueller-Hinton medium at 37°C. Bacteria were stained by the green-fluorescent stain Syto-9 (nucleic acid probe), and nAgCSs were visualized in laser (640 nm) reflection mode (nAgCSs were not detected). All the scale bars are 5 µm.

    Techniques Used: Confocal Laser Scanning Microscopy, Bacteria, Staining

    bacteria e coli atcc 25922  (ATCC)


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    ATCC bacteria e coli atcc 25922

    Bacteria E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?"

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    Journal: bioRxiv

    doi: 10.1101/2024.06.13.596383


    Figure Legend Snippet:

    Techniques Used: Inhibition

    bac against e coli atcc 25922  (ATCC)


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    ATCC bac against e coli atcc 25922

    Bac Against E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?"

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    Journal: bioRxiv

    doi: 10.1101/2024.06.13.596383


    Figure Legend Snippet:

    Techniques Used: Inhibition

    e coli atcc 25922  (ATCC)


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    ATCC e coli atcc 25922
    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli atcc 25922
    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, <t>E.</t> <t>coli</t> ATCC 25922.
    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bacteria e coli atcc 25922

    Bacteria E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bac against e coli atcc 25922

    Bac Against E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.

    Journal: Nature Communications

    Article Title: Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target

    doi: 10.1038/s41467-024-49484-7

    Figure Lengend Snippet: a General structures of hydrazine fragment moiety are shown. Each hydrazine class are represented by different colours (green; BZ, cyan; PA, grey; AC, yellow; AA, orange; LA). b–h MraY inhibitory activity and antibacterial activity of the hydrazone library. The bar graphs show the relative enzyme activity with DMSO as 100% when the compound is treated at given concentration (b,c; TUN at 100 nM, d,e; MRY at 10 nM, f.g; MRD at 100 nM, h; CAP at 1000 nM. n = 1), with the lower bars indicating higher inhibitory activity. The colour of the bars corresponds to Fig. 2a. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) at three points, 0.5, 5, and 50 μM ( n = 1), and shown as a heat map with the compound on the horizontal axis and the bacterium on the vertical axis. The redder the heat map, the lower the MIC value, i.e., the higher the antibacterial activity (pink; <0.5 µM, orange; 5 µM, yellow orange; 50 µM, light grey; >50 µM). Data of control compounds (van: vancomycin, col: colistin) are shown left column in each panel. E. faecium ATCC 35667, S. aureus ATCC 29213, P. aeruginosa ATCC 27853, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047, A. baumannii ATCC 19606, E. coli ATCC 25922.

    Article Snippet: The bactericidal activity of 2 against S. aureus ATCC 29213 and E. coli ATCC 25922 was determined using the time-kill method according to the CLSI guideline.

    Techniques: Activity Assay, Concentration Assay

    MraY inhibitory and antibacterial activity against ESKAPE pathogens

    Journal: Nature Communications

    Article Title: Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target

    doi: 10.1038/s41467-024-49484-7

    Figure Lengend Snippet: MraY inhibitory and antibacterial activity against ESKAPE pathogens

    Article Snippet: The bactericidal activity of 2 against S. aureus ATCC 29213 and E. coli ATCC 25922 was determined using the time-kill method according to the CLSI guideline.

    Techniques: Activity Assay

    Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.

    Journal: Nature Communications

    Article Title: Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target

    doi: 10.1038/s41467-024-49484-7

    Figure Lengend Snippet: Time-killing curve against S. aureus ATCC 29213 ( a ) and E. coli ATCC 25922 ( b ) not treated (black circle) or treated with MIC×1/4 (blue diamond), MIC×1 (orange triangle), and MIC×4 (grey square). These data were collected from three independent experiment ( n = 3). Error bars represents SD. c Resistance emergency plot against S. aureus ATCC 29213 treated with MIC×1/2 drugs (rifampicin 0.004 μg/mL (black triangle), vancomycin 0.5 μg/mL (orange circle), or compound 2 0.5 μg/mL (blue diamond)). d The efficacy of analogues against S. aureus (ATCC 29213) mouse thigh infection model. The dots represent the raw data, bar graph indicates average values ( n = 4,5 biologically independent mice). Error bars represents SD. Dose (mg/kg) was shown in parenthesis after the compounds’ name or numbers.

    Article Snippet: The bactericidal activity of 2 against S. aureus ATCC 29213 and E. coli ATCC 25922 was determined using the time-kill method according to the CLSI guideline.

    Techniques: Analogues, Infection

    Journal: bioRxiv

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    doi: 10.1101/2024.06.13.596383

    Figure Lengend Snippet:

    Article Snippet: The antimicrobial efficiency of nAgCS-1, nAgCS-3, AgNO 3 and low molecular weight chitosan (LMW CS) against bacteria E. coli ATCC 25922 and S. aureus ATCC 6538 was evaluated according to the antimicrobial susceptibility test protocol (ISO 20776-1:2019 [ ]).

    Techniques: Inhibition

    Journal: bioRxiv

    Article Title: Do silver-chitosan nanocomposites promote bacterial resistance to silver or common antibiotics?

    doi: 10.1101/2024.06.13.596383

    Figure Lengend Snippet:

    Article Snippet: Interestingly, the combination of silver and chitosan in the ratio of 1:3 yielded nanocomposites that were as efficient as AgNO 3 against S. aureus ATCC 6538 (MIC 6.25 mg Ag/L) and as efficient as BAC against E. coli ATCC 25922 (MIC 6.25 mg/L).

    Techniques: Inhibition