e coli atcc 25922  (ATCC)


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    Name:
    Escherichia coli
    Description:

    Catalog Number:
    25922-MINI-PACK
    Price:
    None
    Applications:
    ATCC® 25922-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 25922™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Learn more about ATCC® MinisDeveloped by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! . Susceptibility disc testing cephalexin ref Susceptibility disc testing cephaloglycin ref Susceptibility disc testing kanamycin ref Susceptibility disc testing cephaloridine cephalomycin ref ref Food testing Control Culture ref ref ref Control strain ref ref ref Evaluation of Mueller-Hinton agar ref ref Media testing ref ref ref ref ref ref ref Quality control strain ref ref ref ref ref ref ref ref ref ref ref Susceptibility disc testing ref ref ref ref Susceptibility disc testing cephalothin ref Susceptibility disc testing chloramphenicol ref Susceptibility disc testing colistin colimycin ref Susceptibility disc testing gentamicins gentamicin ref Susceptibility disc testing nalidixic acid ref Susceptibility disc testing neomycin ref Susceptibility disc testing tetracycline ref Susceptibility testing ref ref ref ref ref ref ref ref ref ref Testing ref Quality control strain for Abbott, API, Autobac, BBL, bioMérieux Vitek, Biosynth, Difco, IDS, Micro-Media, MicroScan®, Roche Diagnostics, and Sensititre products
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    Structured Review

    ATCC e coli atcc 25922
    Antibacterial activity of AgNPs against different bacteria ( E .  coli  ATCC 25922,  S. aureus  ATCC 25923,  B. subtilis ,  P. aeruginosa ,  K. pneumoniae  AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

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    Images

    1) Product Images from "Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria"

    Article Title: Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-18590-6

    Antibacterial activity of AgNPs against different bacteria ( E .  coli  ATCC 25922,  S. aureus  ATCC 25923,  B. subtilis ,  P. aeruginosa ,  K. pneumoniae  AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.
    Figure Legend Snippet: Antibacterial activity of AgNPs against different bacteria ( E . coli ATCC 25922, S. aureus ATCC 25923, B. subtilis , P. aeruginosa , K. pneumoniae AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

    Techniques Used: Activity Assay

    2) Product Images from "White-Light-Activated Antibacterial Surfaces Generated by Synergy between Zinc Oxide Nanoparticles and Crystal Violet"

    Article Title: White-Light-Activated Antibacterial Surfaces Generated by Synergy between Zinc Oxide Nanoparticles and Crystal Violet

    Journal: ACS Omega

    doi: 10.1021/acsomega.7b01473

    Number of viable colony counts of E. coli ATCC 25922 on treated PDMS squares after incubation in the dark and in white light for 45 min. The purple asterisk indicates E. coli levels below detection limits.
    Figure Legend Snippet: Number of viable colony counts of E. coli ATCC 25922 on treated PDMS squares after incubation in the dark and in white light for 45 min. The purple asterisk indicates E. coli levels below detection limits.

    Techniques Used: Incubation

    3) Product Images from "New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp. Dairy Isolates"

    Article Title: New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp. Dairy Isolates

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.00078

    Association of Salmonella Enteritidis 654/7E to HT29-MTX cells in the presence of enterococci (A) and association of E. coli ATCC 25922 to HT29-MTX cells in the presence of enterococci (B) . The statistical differences with respect to the control strains are annotated with asterisks (* p
    Figure Legend Snippet: Association of Salmonella Enteritidis 654/7E to HT29-MTX cells in the presence of enterococci (A) and association of E. coli ATCC 25922 to HT29-MTX cells in the presence of enterococci (B) . The statistical differences with respect to the control strains are annotated with asterisks (* p

    Techniques Used:

    4) Product Images from "Pluronic-based nano-self-assemblies of bacitracin A with a new mechanism of action for an efficient in vivo therapeutic effect against bacterial peritonitis"

    Article Title: Pluronic-based nano-self-assemblies of bacitracin A with a new mechanism of action for an efficient in vivo therapeutic effect against bacterial peritonitis

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-018-0397-3

    Δπ–π i relationships in constant area mode induced by the injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( a ). Relative surface area change (%) upon injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( b ). The binding affinity of Nano-BA P85 to LPS from E. coli ATCC 25922 was determined by the BODIPY-TR-cadaverine displacement method ( c ). Size distribution of LPS. ( d ) and size distribution of LPS in the presence of Nano-BA P85 ( e ). Representative results from six individual observations are shown as the mean ± SD in each group
    Figure Legend Snippet: Δπ–π i relationships in constant area mode induced by the injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( a ). Relative surface area change (%) upon injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( b ). The binding affinity of Nano-BA P85 to LPS from E. coli ATCC 25922 was determined by the BODIPY-TR-cadaverine displacement method ( c ). Size distribution of LPS. ( d ) and size distribution of LPS in the presence of Nano-BA P85 ( e ). Representative results from six individual observations are shown as the mean ± SD in each group

    Techniques Used: Injection, Binding Assay

    TEM micrographs of E. coli ATCC 25922 treated with the Negative control ( A ) Nano-BA PLGA ( B ) Nano-BA P85 with an incubation time of 30 min ( C ) 1 h ( D ) 2 h ( E ) and Polymyxin B ( F )
    Figure Legend Snippet: TEM micrographs of E. coli ATCC 25922 treated with the Negative control ( A ) Nano-BA PLGA ( B ) Nano-BA P85 with an incubation time of 30 min ( C ) 1 h ( D ) 2 h ( E ) and Polymyxin B ( F )

    Techniques Used: Transmission Electron Microscopy, Negative Control, Incubation

    5) Product Images from "Pluronic-based nano-self-assemblies of bacitracin A with a new mechanism of action for an efficient in vivo therapeutic effect against bacterial peritonitis"

    Article Title: Pluronic-based nano-self-assemblies of bacitracin A with a new mechanism of action for an efficient in vivo therapeutic effect against bacterial peritonitis

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-018-0397-3

    Δπ–π i relationships in constant area mode induced by the injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( a ). Relative surface area change (%) upon injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( b ). The binding affinity of Nano-BA P85 to LPS from E. coli ATCC 25922 was determined by the BODIPY-TR-cadaverine displacement method ( c ). Size distribution of LPS. ( d ) and size distribution of LPS in the presence of Nano-BA P85 ( e ). Representative results from six individual observations are shown as the mean ± SD in each group
    Figure Legend Snippet: Δπ–π i relationships in constant area mode induced by the injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( a ). Relative surface area change (%) upon injection of Nano-BA 5K (0.1 μM), Nano-BA 5K (1 μM), Nano-BA P85 (0.1 μM) and Nano-BA P85 (1 μM) beneath the mixed LPS 3:1 monolayers ( b ). The binding affinity of Nano-BA P85 to LPS from E. coli ATCC 25922 was determined by the BODIPY-TR-cadaverine displacement method ( c ). Size distribution of LPS. ( d ) and size distribution of LPS in the presence of Nano-BA P85 ( e ). Representative results from six individual observations are shown as the mean ± SD in each group

    Techniques Used: Injection, Binding Assay

    TEM micrographs of E. coli ATCC 25922 treated with the Negative control ( A ) Nano-BA PLGA ( B ) Nano-BA P85 with an incubation time of 30 min ( C ) 1 h ( D ) 2 h ( E ) and Polymyxin B ( F )
    Figure Legend Snippet: TEM micrographs of E. coli ATCC 25922 treated with the Negative control ( A ) Nano-BA PLGA ( B ) Nano-BA P85 with an incubation time of 30 min ( C ) 1 h ( D ) 2 h ( E ) and Polymyxin B ( F )

    Techniques Used: Transmission Electron Microscopy, Negative Control, Incubation

    6) Product Images from "Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli"

    Article Title: Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli

    Journal: Science Advances

    doi: 10.1126/sciadv.aau2634

    Photolabeling of E. coli ATCC 25922 with thanatin-PAL5. ( A ) Western blot (biotin detection) and corresponding SDS–polyacrylamide gel electrophoresis (SDS-PAGE) (Coomassie blue staining) of membrane protein fraction from: lane 1, control unlabeled cells; lanes 2 and 3, cells photolabeled with thanatin-PAL5 (10 and 2 μg/ml); and lane 4, cells photolabeled with thanatin-PAL5 (10 μg/ml) + competitor thanatin (200 μg/ml). ( B ) Western blot and SDS-PAGE after photolabeling with thanatin-PAL5 (2 μg/ml) with (+) or without (−) reduction of extracted membrane proteins with dithiothreitol (DTT). ( C ) Volcano plot showing relative abundance of E. coli proteins in thanatin-PAL5 labeled versus unlabeled control sample after streptavidin pulldown detected by MS-based proteomic analysis. Significantly enriched proteins (right/above dashed lines) are highlighted in green and represent PAL5-labeled proteins.
    Figure Legend Snippet: Photolabeling of E. coli ATCC 25922 with thanatin-PAL5. ( A ) Western blot (biotin detection) and corresponding SDS–polyacrylamide gel electrophoresis (SDS-PAGE) (Coomassie blue staining) of membrane protein fraction from: lane 1, control unlabeled cells; lanes 2 and 3, cells photolabeled with thanatin-PAL5 (10 and 2 μg/ml); and lane 4, cells photolabeled with thanatin-PAL5 (10 μg/ml) + competitor thanatin (200 μg/ml). ( B ) Western blot and SDS-PAGE after photolabeling with thanatin-PAL5 (2 μg/ml) with (+) or without (−) reduction of extracted membrane proteins with dithiothreitol (DTT). ( C ) Volcano plot showing relative abundance of E. coli proteins in thanatin-PAL5 labeled versus unlabeled control sample after streptavidin pulldown detected by MS-based proteomic analysis. Significantly enriched proteins (right/above dashed lines) are highlighted in green and represent PAL5-labeled proteins.

    Techniques Used: Western Blot, Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Labeling, Mass Spectrometry

    Electron and fluorescence microscopy studies. ( A and B ) TEM studies of E. coli ATCC 25922, before (A) and after (B) thanatin treatment (1.5 μg/ml), showing internal accumulations of membrane-like material. Scale bars, 500 nm. ( C and D ) Super-resolution fluorescence microscopy of E. coli ATCC 25922 without (C) or with thanatin (5 μg/ml) (D) and stained with FM4-64, SYTOX Green, or DAPI. Top: The FM4-64 channel (red staining). Bottom: Superimposition of all three channels [with DAPI (blue) and SYTOX Green (nondetected)]. ( E ) E. coli staining with thanatin-BDP-FL (8 μg/ml) for 2 hours at 30°C (both pictures). Cells were analyzed using a Leica CLSM SP8 gSTED microscope. Scale bars, 4 or 10 μm (bottom right). For experimental details, see section S5.
    Figure Legend Snippet: Electron and fluorescence microscopy studies. ( A and B ) TEM studies of E. coli ATCC 25922, before (A) and after (B) thanatin treatment (1.5 μg/ml), showing internal accumulations of membrane-like material. Scale bars, 500 nm. ( C and D ) Super-resolution fluorescence microscopy of E. coli ATCC 25922 without (C) or with thanatin (5 μg/ml) (D) and stained with FM4-64, SYTOX Green, or DAPI. Top: The FM4-64 channel (red staining). Bottom: Superimposition of all three channels [with DAPI (blue) and SYTOX Green (nondetected)]. ( E ) E. coli staining with thanatin-BDP-FL (8 μg/ml) for 2 hours at 30°C (both pictures). Cells were analyzed using a Leica CLSM SP8 gSTED microscope. Scale bars, 4 or 10 μm (bottom right). For experimental details, see section S5.

    Techniques Used: Fluorescence, Microscopy, Transmission Electron Microscopy, Staining, Confocal Laser Scanning Microscopy

    7) Product Images from "Urinary Concentrations and Antibacterial Activities of Nitroxoline at 250 Milligrams versus Trimethoprim at 200 Milligrams against Uropathogens in Healthy Volunteers"

    Article Title: Urinary Concentrations and Antibacterial Activities of Nitroxoline at 250 Milligrams versus Trimethoprim at 200 Milligrams against Uropathogens in Healthy Volunteers

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.02147-13

    Urinary bactericidal kinetics for E. coli ATCC 25922 in volunteers A, B, and C, using urine samples collected 0 to 2 h after the eighth dose (a) and 4 to 6 h after the 10th dose (b) of nitroxoline at 250 mg t.i.d.
    Figure Legend Snippet: Urinary bactericidal kinetics for E. coli ATCC 25922 in volunteers A, B, and C, using urine samples collected 0 to 2 h after the eighth dose (a) and 4 to 6 h after the 10th dose (b) of nitroxoline at 250 mg t.i.d.

    Techniques Used:

    8) Product Images from "Urinary Concentrations and Antibacterial Activities of Nitroxoline at 250 Milligrams versus Trimethoprim at 200 Milligrams against Uropathogens in Healthy Volunteers"

    Article Title: Urinary Concentrations and Antibacterial Activities of Nitroxoline at 250 Milligrams versus Trimethoprim at 200 Milligrams against Uropathogens in Healthy Volunteers

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.02147-13

    Urinary bactericidal kinetics for E. coli ATCC 25922 in volunteers A, B, and C, using urine samples collected 0 to 2 h after the eighth dose (a) and 4 to 6 h after the 10th dose (b) of nitroxoline at 250 mg t.i.d.
    Figure Legend Snippet: Urinary bactericidal kinetics for E. coli ATCC 25922 in volunteers A, B, and C, using urine samples collected 0 to 2 h after the eighth dose (a) and 4 to 6 h after the 10th dose (b) of nitroxoline at 250 mg t.i.d.

    Techniques Used:

    9) Product Images from "Galactomannan from Trigonella foenum‐graecum L. seed: Prebiotic application and its fermentation by the probiotic Bacillus coagulans strain MTCC 5856, et al. Galactomannan from Trigonella foenum‐graecum L. seed: Prebiotic application and its fermentation by the probiotic Bacillus coagulans strain MTCC 5856"

    Article Title: Galactomannan from Trigonella foenum‐graecum L. seed: Prebiotic application and its fermentation by the probiotic Bacillus coagulans strain MTCC 5856, et al. Galactomannan from Trigonella foenum‐graecum L. seed: Prebiotic application and its fermentation by the probiotic Bacillus coagulans strain MTCC 5856

    Journal: Food Science & Nutrition

    doi: 10.1002/fsn3.606

    The effect of Bacillus coagulans MTCC 5856 on the viability of Escherichia coli ATCC 25922 from galactomannan from fenugreek seeds alone and along with MRSD ( MRS media devoid of dextrose), MRS , and MRS media devoid of dextrose. Values are average mean of triplicate performed at two different occasions and represented in log 10 cfu/ml
    Figure Legend Snippet: The effect of Bacillus coagulans MTCC 5856 on the viability of Escherichia coli ATCC 25922 from galactomannan from fenugreek seeds alone and along with MRSD ( MRS media devoid of dextrose), MRS , and MRS media devoid of dextrose. Values are average mean of triplicate performed at two different occasions and represented in log 10 cfu/ml

    Techniques Used:

    10) Product Images from "In Vivo Pharmacodynamic Characterization of a Novel Odilorhabdin Antibiotic, NOSO-502, against Escherichia coli and Klebsiella pneumoniae in a Murine Thigh Infection Model"

    Article Title: In Vivo Pharmacodynamic Characterization of a Novel Odilorhabdin Antibiotic, NOSO-502, against Escherichia coli and Klebsiella pneumoniae in a Murine Thigh Infection Model

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01067-18

    In vivo  dose fractionation with NOSO-502 using a neutropenic mouse thigh model. Each symbol represents the mean from four thighs infected with  E. coli  ATCC 25922. The error bars represent the SDs. The burden of organisms was measured at the start and end of therapy. Five total drug (milligrams per kilogram per 24 h) dose levels were fractionated into one of four dosing regimens and are shown on the  x  axis. The  y  axis represents the change in organism burden from the start of therapy. The dashed horizontal line represents net stasis over the treatment period. Points above the line represent net growth, and points below represent net killing (bactericidal activity).
    Figure Legend Snippet: In vivo dose fractionation with NOSO-502 using a neutropenic mouse thigh model. Each symbol represents the mean from four thighs infected with E. coli ATCC 25922. The error bars represent the SDs. The burden of organisms was measured at the start and end of therapy. Five total drug (milligrams per kilogram per 24 h) dose levels were fractionated into one of four dosing regimens and are shown on the x axis. The y axis represents the change in organism burden from the start of therapy. The dashed horizontal line represents net stasis over the treatment period. Points above the line represent net growth, and points below represent net killing (bactericidal activity).

    Techniques Used: In Vivo, Fractionation, Infection, Activity Assay

    Impact of pharmacodynamic regression of the  in vivo  dose fractionation study with NOSO-502 against  E. coli  ATCC 25922. Each symbol represents the mean from four thighs. The dose data are expressed as free drug AUC/MIC ( f AUC/MIC), free drug  C max /MIC ( fC max /MIC), and the percent time that plasma free drug concentrations exceeded the MIC (% T MIC ).  R 2  is the coefficient of determination. Also shown for each PD index is the maximal effect ( E max ), the PD index value associated with 50% of the maximal effect (ED 50 ), and the slope of the relationship, or the Hill coefficient ( N ). The line drawn through the data points is the best-fit line based upon the sigmoid  E max  formula.
    Figure Legend Snippet: Impact of pharmacodynamic regression of the in vivo dose fractionation study with NOSO-502 against E. coli ATCC 25922. Each symbol represents the mean from four thighs. The dose data are expressed as free drug AUC/MIC ( f AUC/MIC), free drug C max /MIC ( fC max /MIC), and the percent time that plasma free drug concentrations exceeded the MIC (% T MIC ). R 2 is the coefficient of determination. Also shown for each PD index is the maximal effect ( E max ), the PD index value associated with 50% of the maximal effect (ED 50 ), and the slope of the relationship, or the Hill coefficient ( N ). The line drawn through the data points is the best-fit line based upon the sigmoid E max formula.

    Techniques Used: In Vivo, Fractionation

    11) Product Images from "Newly Isolated Paenibacillus tyrfis sp. nov., from Malaysian Tropical Peat Swamp Soil with Broad Spectrum Antimicrobial Activity"

    Article Title: Newly Isolated Paenibacillus tyrfis sp. nov., from Malaysian Tropical Peat Swamp Soil with Broad Spectrum Antimicrobial Activity

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.00219

    Scanning electron microscopy images on the antimicrobial effect of active fraction produced by strain MSt1 T against E. coli ATCC 25922 at 5000x magnification . (A) Negative control (treatment with PBS); (B) Treatment with 0.1 mg/mL of active fraction produced by strain MSt1 T . Arrows points at the cell rupture of E. coli after treatment.
    Figure Legend Snippet: Scanning electron microscopy images on the antimicrobial effect of active fraction produced by strain MSt1 T against E. coli ATCC 25922 at 5000x magnification . (A) Negative control (treatment with PBS); (B) Treatment with 0.1 mg/mL of active fraction produced by strain MSt1 T . Arrows points at the cell rupture of E. coli after treatment.

    Techniques Used: Electron Microscopy, Produced, Negative Control

    12) Product Images from "Coupling next-generation sequencing to dominant positive screens for finding antibiotic cellular targets and resistance mechanisms in Escherichia coli"

    Article Title: Coupling next-generation sequencing to dominant positive screens for finding antibiotic cellular targets and resistance mechanisms in Escherichia coli

    Journal: Microbial Genomics

    doi: 10.1099/mgen.0.000148

    CRISPRi knock-down of the E. coli rob gene. Quantitative RT-PCR showing relative rob expression compared with that in wild-type E. coli ATCC 25922. dCas9, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease; rob sgRNA, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and a single guide RNA against the rob gene; rob sgRNA mismatch, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and an inactive version of the single guide RNA against the rob gene. All qRT-PCR data were normalized according to the amplification signals of the housekeeping tuf mRNA. MICs for CRO measured by agar dilution are shown for all strains. Error bars indicate the standard deviation for the triplicate measurements.
    Figure Legend Snippet: CRISPRi knock-down of the E. coli rob gene. Quantitative RT-PCR showing relative rob expression compared with that in wild-type E. coli ATCC 25922. dCas9, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease; rob sgRNA, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and a single guide RNA against the rob gene; rob sgRNA mismatch, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and an inactive version of the single guide RNA against the rob gene. All qRT-PCR data were normalized according to the amplification signals of the housekeeping tuf mRNA. MICs for CRO measured by agar dilution are shown for all strains. Error bars indicate the standard deviation for the triplicate measurements.

    Techniques Used: Quantitative RT-PCR, Expressing, Amplification, Standard Deviation

    13) Product Images from "Coupling next-generation sequencing to dominant positive screens for finding antibiotic cellular targets and resistance mechanisms in Escherichia coli"

    Article Title: Coupling next-generation sequencing to dominant positive screens for finding antibiotic cellular targets and resistance mechanisms in Escherichia coli

    Journal: Microbial Genomics

    doi: 10.1099/mgen.0.000148

    CRISPRi knock-down of the E. coli rob gene. Quantitative RT-PCR showing relative rob expression compared with that in wild-type E. coli ATCC 25922. dCas9, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease; rob sgRNA, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and a single guide RNA against the rob gene; rob sgRNA mismatch, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and an inactive version of the single guide RNA against the rob gene. All qRT-PCR data were normalized according to the amplification signals of the housekeeping tuf mRNA. MICs for CRO measured by agar dilution are shown for all strains. Error bars indicate the standard deviation for the triplicate measurements.
    Figure Legend Snippet: CRISPRi knock-down of the E. coli rob gene. Quantitative RT-PCR showing relative rob expression compared with that in wild-type E. coli ATCC 25922. dCas9, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease; rob sgRNA, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and a single guide RNA against the rob gene; rob sgRNA mismatch, E. coli ATCC 25922 expressing a catalytically dead version of the Cas9 nuclease and an inactive version of the single guide RNA against the rob gene. All qRT-PCR data were normalized according to the amplification signals of the housekeeping tuf mRNA. MICs for CRO measured by agar dilution are shown for all strains. Error bars indicate the standard deviation for the triplicate measurements.

    Techniques Used: Quantitative RT-PCR, Expressing, Amplification, Standard Deviation

    14) Product Images from "Ag colloids and Ag clusters over EDAPTMS-coated silica nanoparticles: synthesis, characterization, and antibacterial activity against Escherichia coli"

    Article Title: Ag colloids and Ag clusters over EDAPTMS-coated silica nanoparticles: synthesis, characterization, and antibacterial activity against Escherichia coli

    Journal: Nanomedicine : nanotechnology, biology, and medicine

    doi: 10.1016/j.nano.2010.11.003

    Fluorescent microscopy images of live and dead bacterial cells in presence of silver NPs, using green and red UV-visible filters, respectively.  (A ,  B)  100 μg/mL Ag-SiO 2  NPs +  E. coli  ATCC 25922;  (C ,  D)  8.5 μg/mL Ag colloid NPs +  E. coli
    Figure Legend Snippet: Fluorescent microscopy images of live and dead bacterial cells in presence of silver NPs, using green and red UV-visible filters, respectively. (A , B) 100 μg/mL Ag-SiO 2 NPs + E. coli ATCC 25922; (C , D) 8.5 μg/mL Ag colloid NPs + E. coli

    Techniques Used: Microscopy

    Growth curve of E. coli (EC) ATCC 25922 (A) in presence of Ag-c NPs at a concentration of 0.85 ( dark blue ), 8.5 ( pink ), and 42.5 μg/mL ( red ) with their positive controls (bacteria only) light blue, yellow, and violet, respectively, and negative
    Figure Legend Snippet: Growth curve of E. coli (EC) ATCC 25922 (A) in presence of Ag-c NPs at a concentration of 0.85 ( dark blue ), 8.5 ( pink ), and 42.5 μg/mL ( red ) with their positive controls (bacteria only) light blue, yellow, and violet, respectively, and negative

    Techniques Used: Concentration Assay

    Colonies count of E. coli ATCC 25922 expressed as a percentage of the number of colonies grown on silver-free LB agar plates. (A) Ag-SiO 2 NPs at 0.1, 1, 10, and 100 μg/mL; (B) colloidal Ag NPs at 0.085, 0.85, 8.5, and 42.5 μg/mL. (C ,
    Figure Legend Snippet: Colonies count of E. coli ATCC 25922 expressed as a percentage of the number of colonies grown on silver-free LB agar plates. (A) Ag-SiO 2 NPs at 0.1, 1, 10, and 100 μg/mL; (B) colloidal Ag NPs at 0.085, 0.85, 8.5, and 42.5 μg/mL. (C ,

    Techniques Used:

    Bar graph of live and dead  (A) E. coli  ATCC 25922,  (B) E. coli  O157:H7 in the presence and absence of Ag-c NPs, where bacterial cells counts are expressed as a function of percentage per milliliter.
    Figure Legend Snippet: Bar graph of live and dead (A) E. coli ATCC 25922, (B) E. coli O157:H7 in the presence and absence of Ag-c NPs, where bacterial cells counts are expressed as a function of percentage per milliliter.

    Techniques Used:

    15) Product Images from "Broad-Spectrum Activity against Bacterial Mastitis Pathogens and Activation of Mammary Epithelial Cells Support a Protective Role of Neutrophil Cathelicidins in Bovine Mastitis ▿"

    Article Title: Broad-Spectrum Activity against Bacterial Mastitis Pathogens and Activation of Mammary Epithelial Cells Support a Protective Role of Neutrophil Cathelicidins in Bovine Mastitis ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.01090-09

    Killing of E. coli ATCC 25922 in milk collected from cases of clinical mastitis. Bacteria (4 × 10 4 to 7 × 10 4 CFU/ml) were incubated in mastitic milk samples (a to f) at 37°C for 60 min in the absence and presence of BMAP-27, BMAP-28,
    Figure Legend Snippet: Killing of E. coli ATCC 25922 in milk collected from cases of clinical mastitis. Bacteria (4 × 10 4 to 7 × 10 4 CFU/ml) were incubated in mastitic milk samples (a to f) at 37°C for 60 min in the absence and presence of BMAP-27, BMAP-28,

    Techniques Used: Incubation

    16) Product Images from "Transcriptional response of OmpC and OmpF in Escherichia coli against differential gradient of carbapenem stress"

    Article Title: Transcriptional response of OmpC and OmpF in Escherichia coli against differential gradient of carbapenem stress

    Journal: BMC Research Notes

    doi: 10.1186/s13104-019-4177-4

    Expression of OmpF, OmpC and micF gene under normal condition (without stress) relative to E. coli ATCC 25922
    Figure Legend Snippet: Expression of OmpF, OmpC and micF gene under normal condition (without stress) relative to E. coli ATCC 25922

    Techniques Used: Expressing

    17) Product Images from "Data on Laurdan spectroscopic analyses to compare membrane fluidity between susceptible and multidrug-resistant bacteria"

    Article Title: Data on Laurdan spectroscopic analyses to compare membrane fluidity between susceptible and multidrug-resistant bacteria

    Journal: Data in Brief

    doi: 10.1016/j.dib.2018.09.106

    Laurdan emission spectra in three E. coli strains – E. coli ATCC 25922 and two multidrug-resistant isolates, E. coli EC2 and E. coli EC3 – at 24, 48, 72 and 144 h after bacterial growth at 37 °C. The excitation wavelength was 350 nm. Data presented are from a single experiment. Intensities at 440 and 490 nm were used to calculate excitation generalized polarization values ( GP exc ).
    Figure Legend Snippet: Laurdan emission spectra in three E. coli strains – E. coli ATCC 25922 and two multidrug-resistant isolates, E. coli EC2 and E. coli EC3 – at 24, 48, 72 and 144 h after bacterial growth at 37 °C. The excitation wavelength was 350 nm. Data presented are from a single experiment. Intensities at 440 and 490 nm were used to calculate excitation generalized polarization values ( GP exc ).

    Techniques Used:

    18) Product Images from "Determination of the In Vivo Pharmacodynamic Profile of Cefepime against Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli at Various Inocula"

    Article Title: Determination of the In Vivo Pharmacodynamic Profile of Cefepime against Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli at Various Inocula

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.48.6.1941-1947.2004

    Relationship between the % T > MIC and the change in bacterial density following infection with non-ESBL-producing E. coli strains ATCC 25922 and EC 120 at the standard inoculum (A) and a 100-fold-higher inoculum (B).
    Figure Legend Snippet: Relationship between the % T > MIC and the change in bacterial density following infection with non-ESBL-producing E. coli strains ATCC 25922 and EC 120 at the standard inoculum (A) and a 100-fold-higher inoculum (B).

    Techniques Used: Infection

    19) Product Images from "Detection of KPC Carbapenemase in Pseudomonas aeruginosa Isolated From Clinical Samples Using Modified Hodge Test and Boronic Acid Phenotypic Methods and Their Comparison With the Polymerase Chain Reaction"

    Article Title: Detection of KPC Carbapenemase in Pseudomonas aeruginosa Isolated From Clinical Samples Using Modified Hodge Test and Boronic Acid Phenotypic Methods and Their Comparison With the Polymerase Chain Reaction

    Journal: Jundishapur Journal of Microbiology

    doi: 10.5812/jjm.27249

    A, modified hodge test (MHT). Results for three Pseudomonas isolates, which were unexplainable when using E. coli ATCC 25922 standard strain. B, MHT test results using K. pneumoniae ATCC 700603 standard strain. C, The results of combination-disk test with boronic acid, which was positive for the examined strains.
    Figure Legend Snippet: A, modified hodge test (MHT). Results for three Pseudomonas isolates, which were unexplainable when using E. coli ATCC 25922 standard strain. B, MHT test results using K. pneumoniae ATCC 700603 standard strain. C, The results of combination-disk test with boronic acid, which was positive for the examined strains.

    Techniques Used: Modification

    20) Product Images from "End-Tagging of Ultra-Short Antimicrobial Peptides by W/F Stretches to Facilitate Bacterial Killing"

    Article Title: End-Tagging of Ultra-Short Antimicrobial Peptides by W/F Stretches to Facilitate Bacterial Killing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0005285

    Antibacterial and hemolytic activity of peptides. (A) Antibacterial activity of PEPscreen peptides as assessed by radial diffusion assay (RDA) in the presence and absence of 0.15 M NaCl against E. coli ATCC 25922 and S. aureus ATCC 29213, as well as hemolysis. For determination of antibacterial activity, bacteria (4×10 6 cfu/ml) were inoculated in 0.1% TSB agarose gel and 4 mm wells punched. In each well 6 µl of peptide (at 100 µM) was loaded. The zones of clearance correspond to the inhibitory effect of each peptide after incubation at 37°C for 18–24 h (mean values are presented, n = 3). For hemolysis, the cells were incubated with the peptides at 60 µM, while 2% Triton X-100 served as positive control. The absorbance of hemoglobin release was measured at 540 nm and is expressed as % of Triton X-100 induced hemolysis. (B) Dose dependent activity of selected high purity peptides KNK10, KNK7, and W-modified variants of these against E. coli ATCC 25922 and S. aureus ATCC 29213 in RDA. Shown also are effects of the peptides on human erythrocytes in the hemolysis assay. Throughout, mean values are presented, n = 3. (In Figure 1B , the difference between tagged and non-tagged peptides is statistically significant in all cases (P
    Figure Legend Snippet: Antibacterial and hemolytic activity of peptides. (A) Antibacterial activity of PEPscreen peptides as assessed by radial diffusion assay (RDA) in the presence and absence of 0.15 M NaCl against E. coli ATCC 25922 and S. aureus ATCC 29213, as well as hemolysis. For determination of antibacterial activity, bacteria (4×10 6 cfu/ml) were inoculated in 0.1% TSB agarose gel and 4 mm wells punched. In each well 6 µl of peptide (at 100 µM) was loaded. The zones of clearance correspond to the inhibitory effect of each peptide after incubation at 37°C for 18–24 h (mean values are presented, n = 3). For hemolysis, the cells were incubated with the peptides at 60 µM, while 2% Triton X-100 served as positive control. The absorbance of hemoglobin release was measured at 540 nm and is expressed as % of Triton X-100 induced hemolysis. (B) Dose dependent activity of selected high purity peptides KNK10, KNK7, and W-modified variants of these against E. coli ATCC 25922 and S. aureus ATCC 29213 in RDA. Shown also are effects of the peptides on human erythrocytes in the hemolysis assay. Throughout, mean values are presented, n = 3. (In Figure 1B , the difference between tagged and non-tagged peptides is statistically significant in all cases (P

    Techniques Used: Activity Assay, Diffusion-based Assay, Agarose Gel Electrophoresis, Incubation, Positive Control, Modification, Hemolysis Assay

    (A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), V8 proteinase (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In Figure 5B , the difference between tagged and non-tagged peptide is statistically significant in all cases (P
    Figure Legend Snippet: (A) Protease sensitivity of peptides. KNK10 and KNK10-WWWWW were incubated with (+) or without (−) the S. aureus enzymes aureolysin (Aur), V8 proteinase (V8), or human leukocyte elastase (HLE), and analyzed by SDS-PAGE (16.5% Tris-Tricine gels). (B) Activities of peptides in an ex vivo skin infection model. Pig skin was inoculated with E. coli ATCC 25922 (upper panel) or S. aureus ATCC 29213 (lower panel). Peptides at 1 mM were added after an incubation time of 4 h. Bacteria were collected after 2 h and cfu determined (mean values are presented, n = 6). Note the logarithmic scale on the y-axis. (In Figure 5B , the difference between tagged and non-tagged peptide is statistically significant in all cases (P

    Techniques Used: Incubation, SDS Page, Ex Vivo, Infection

    21) Product Images from "Purification, Characterization and in vitro Evaluation of Polymyxin A From Paenibacillus dendritiformis: An Underexplored Member of the Polymyxin Family"

    Article Title: Purification, Characterization and in vitro Evaluation of Polymyxin A From Paenibacillus dendritiformis: An Underexplored Member of the Polymyxin Family

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.02864

    Time-kill kinetics of P1 and P2 against (A) E. coli ATCC 25922 and (B) P. aeruginosa ATCC 27853. The experiment was carried out in triplicate and two biological repeats were performed. Data plotted as mean ± SD.
    Figure Legend Snippet: Time-kill kinetics of P1 and P2 against (A) E. coli ATCC 25922 and (B) P. aeruginosa ATCC 27853. The experiment was carried out in triplicate and two biological repeats were performed. Data plotted as mean ± SD.

    Techniques Used:

    22) Product Images from "In Vitro Antibacterial Activity of Biological-Derived Silver Nanoparticles: Preliminary Data"

    Article Title: In Vitro Antibacterial Activity of Biological-Derived Silver Nanoparticles: Preliminary Data

    Journal: Veterinary Sciences

    doi: 10.3390/vetsci7010012

    Biosynthesis of silver nanoparticles. Plant-mediated method ( A ) after dissolving  C. longa  powder in ddH 2 O, the solution was filtered to remove the majorities of vegetal debris; finally, silver nitrate was added and the change in colour was monitored. Bacterial supernatant method ( B ). After growing  E. coli  ATCC ®  25922 in Mueller-Hinton broth, the supernatant was collected, and silver nitrate was added to induce nanoparticle synthesis.
    Figure Legend Snippet: Biosynthesis of silver nanoparticles. Plant-mediated method ( A ) after dissolving C. longa powder in ddH 2 O, the solution was filtered to remove the majorities of vegetal debris; finally, silver nitrate was added and the change in colour was monitored. Bacterial supernatant method ( B ). After growing E. coli ATCC ® 25922 in Mueller-Hinton broth, the supernatant was collected, and silver nitrate was added to induce nanoparticle synthesis.

    Techniques Used:

    23) Product Images from "Transcriptional response of OmpC and OmpF in Escherichia coli against differential gradient of carbapenem stress"

    Article Title: Transcriptional response of OmpC and OmpF in Escherichia coli against differential gradient of carbapenem stress

    Journal: BMC Research Notes

    doi: 10.1186/s13104-019-4177-4

    Expression of OmpF, OmpC and micF gene under normal condition (without stress) relative to E. coli ATCC 25922
    Figure Legend Snippet: Expression of OmpF, OmpC and micF gene under normal condition (without stress) relative to E. coli ATCC 25922

    Techniques Used: Expressing

    24) Product Images from "Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats"

    Article Title: Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087150

    Influence of a non-probiotic bacteria on the pharmacokinetics of amiodarone (A) and N-desethylamiodarone (B). Legend Fig. 3A: Pharmacokinetics of amiodarone with or without (control group) non-probiotic E. coli ATCC 25922 pre-treatment. Each point is presented as means ± S.D.; N = 3. Legend Fig. 3B: Pharmacokinetics of N-desethylamiodarone (metabolite of amiodarone) with or without (control group) non-probiotic E. coli ATCC 25922 pre-treatment. Each point is presented as means ± S.D.; N = 3.
    Figure Legend Snippet: Influence of a non-probiotic bacteria on the pharmacokinetics of amiodarone (A) and N-desethylamiodarone (B). Legend Fig. 3A: Pharmacokinetics of amiodarone with or without (control group) non-probiotic E. coli ATCC 25922 pre-treatment. Each point is presented as means ± S.D.; N = 3. Legend Fig. 3B: Pharmacokinetics of N-desethylamiodarone (metabolite of amiodarone) with or without (control group) non-probiotic E. coli ATCC 25922 pre-treatment. Each point is presented as means ± S.D.; N = 3.

    Techniques Used:

    25) Product Images from "Enterobactin-Mediated Delivery of β-Lactam Antibiotics Enhances Antibacterial Activity against Pathogenic Escherichia coli"

    Article Title: Enterobactin-Mediated Delivery of β-Lactam Antibiotics Enhances Antibacterial Activity against Pathogenic Escherichia coli

    Journal: Journal of the American Chemical Society

    doi: 10.1021/ja503911p

    Antibacterial activity of Ent-Amp/Amx against various  E. coli  strains that include human pathogens. (A) Laboratory test strain  E. coli  ATCC 25922. (B) Uropathogenic  E. coli  UTI89. (C) Uropathogenic  E. coli  CFT073. (D) Non-pathogenic clinical isolate  E. coli  H9049. (E) Pathogenic ETEC  E. coli  ATCC 35401. (F) Pathogenic EHEC  E. coli  ATCC 43895. All assays were performed in 50% MHB medium supplemented with 200 μM DP to provide iron-limiting conditions (mean ± SEM,  n  ≥ 3). The data for assays performed in the absence of DP are presented in   Figures S2–S7 .
    Figure Legend Snippet: Antibacterial activity of Ent-Amp/Amx against various E. coli strains that include human pathogens. (A) Laboratory test strain E. coli ATCC 25922. (B) Uropathogenic E. coli UTI89. (C) Uropathogenic E. coli CFT073. (D) Non-pathogenic clinical isolate E. coli H9049. (E) Pathogenic ETEC E. coli ATCC 35401. (F) Pathogenic EHEC E. coli ATCC 43895. All assays were performed in 50% MHB medium supplemented with 200 μM DP to provide iron-limiting conditions (mean ± SEM, n ≥ 3). The data for assays performed in the absence of DP are presented in Figures S2–S7 .

    Techniques Used: Activity Assay

    26) Product Images from "Butenolide, a Marine-Derived Broad-Spectrum Antibiofilm Agent Against Both Gram-Positive and Gram-Negative Pathogenic Bacteria"

    Article Title: Butenolide, a Marine-Derived Broad-Spectrum Antibiofilm Agent Against Both Gram-Positive and Gram-Negative Pathogenic Bacteria

    Journal: Marine Biotechnology (New York, N.y.)

    doi: 10.1007/s10126-018-9861-1

    CLSM analyses of biofilm formation inhibitory effects of butenolide (BU). (a)  E. coli  ATCC 25922. (b)  E. coli  K-12. (c)  E. coli  O157:H7. (d)  E. coli  DH5α. (e) strain PAO1. (f) MRSA strain ATCC 43300. Series 1 and 2 were biofilms after 24 h incubation without BU treatment. Series 3 and 4 were biofilms after 24 h incubation treated with BU under MBICs (100 mg/L for  E. coli  K-12, ATCC 25922, and DH5α; 50 mg/L for  E. coli  O157:H7; 800 mg/L for PAO1; 200 mg/L for MRSA). Dyes of SYTO-9 and propidium iodide, wavelength of 488 and 561 nm, and ×20 magnification were used to observe. Scale bar is 50 μm
    Figure Legend Snippet: CLSM analyses of biofilm formation inhibitory effects of butenolide (BU). (a) E. coli ATCC 25922. (b) E. coli K-12. (c) E. coli O157:H7. (d) E. coli DH5α. (e) strain PAO1. (f) MRSA strain ATCC 43300. Series 1 and 2 were biofilms after 24 h incubation without BU treatment. Series 3 and 4 were biofilms after 24 h incubation treated with BU under MBICs (100 mg/L for E. coli K-12, ATCC 25922, and DH5α; 50 mg/L for E. coli O157:H7; 800 mg/L for PAO1; 200 mg/L for MRSA). Dyes of SYTO-9 and propidium iodide, wavelength of 488 and 561 nm, and ×20 magnification were used to observe. Scale bar is 50 μm

    Techniques Used: Confocal Laser Scanning Microscopy, Incubation

    27) Product Images from "Metal Ions, Not Metal-Catalyzed Oxidative Stress, Cause Clay Leachate Antibacterial Activity"

    Article Title: Metal Ions, Not Metal-Catalyzed Oxidative Stress, Cause Clay Leachate Antibacterial Activity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0115172

    CB-L-associated ROS production in the absence and presence of  E. coli  ATCC 25922. ROS was measured in CB-L without the presence of cells (extracellular; using a plate reader) and exclusively in the intracellular environment of exponential-phase  E. coli  following CB-L exposure (intracellular; using flow cytometry). Cells were grown overnight, transferred to fresh growth media and grown to mid-logarithmic phase, washed, and exposed to CB-L for 24 h. Reported values represent the ROS production fold change of CB-L compared to water and CB-L-exposed cells compared to water-exposed cells. Data are presented as the mean ± standard error of the mean (SEM) for three independent experiments.
    Figure Legend Snippet: CB-L-associated ROS production in the absence and presence of E. coli ATCC 25922. ROS was measured in CB-L without the presence of cells (extracellular; using a plate reader) and exclusively in the intracellular environment of exponential-phase E. coli following CB-L exposure (intracellular; using flow cytometry). Cells were grown overnight, transferred to fresh growth media and grown to mid-logarithmic phase, washed, and exposed to CB-L for 24 h. Reported values represent the ROS production fold change of CB-L compared to water and CB-L-exposed cells compared to water-exposed cells. Data are presented as the mean ± standard error of the mean (SEM) for three independent experiments.

    Techniques Used: Flow Cytometry, Cytometry

    Hydrogen peroxide concentration in 10% CB suspensions and CB-L. Hydrogen peroxide levels were measured in 10% CB suspensions and CB-L with or without the addition of stationary-phase  E. coli  ATCC 25922. Values represent the average H 2 O 2  concentration ±SD for three independent experiments.
    Figure Legend Snippet: Hydrogen peroxide concentration in 10% CB suspensions and CB-L. Hydrogen peroxide levels were measured in 10% CB suspensions and CB-L with or without the addition of stationary-phase E. coli ATCC 25922. Values represent the average H 2 O 2 concentration ±SD for three independent experiments.

    Techniques Used: Concentration Assay

    Assessment of E. coli ATCC 25922 oxidative damage to proteins (A), DNA (B), and lipids (C) and killing (D) upon exposure to CB-L and ROS scavengers or metal chelators. (A) Oxidative damage to protein samples collected from E. coli exposed to dH 2 O, H 2 O 2 (2 mM), and CB-L with and without the addition of DMSO (1%) or EDTA (10 mM). Values represent the concentration of total carbonylated protein lesions formed as a result of protein oxidation during control and experimental exposures. Error bars represent the SEM for at least three independent experiments. (B) 8-OHdG levels measured in E. coli following CB-L exposure with and without the addition of DMSO or EDTA. Error bars represent the SEM for at least three independent experiments. (C) Lipid peroxidation levels measured in E. coli exposed to dH 2 O, H 2 O 2 , and CB-L with and without the addition of DMSO or EDTA. Values represent the percent change in lipid peroxidation over the water-exposed control, and the SEM for at least three independent experiments. (D) Viability of logarithmic phase E. coli following CB-L exposure with and without the addition of DMSO or EDTA. Error bars represent the SEM for at least three independent experiments. The dotted line represents the limit of detection. *, p
    Figure Legend Snippet: Assessment of E. coli ATCC 25922 oxidative damage to proteins (A), DNA (B), and lipids (C) and killing (D) upon exposure to CB-L and ROS scavengers or metal chelators. (A) Oxidative damage to protein samples collected from E. coli exposed to dH 2 O, H 2 O 2 (2 mM), and CB-L with and without the addition of DMSO (1%) or EDTA (10 mM). Values represent the concentration of total carbonylated protein lesions formed as a result of protein oxidation during control and experimental exposures. Error bars represent the SEM for at least three independent experiments. (B) 8-OHdG levels measured in E. coli following CB-L exposure with and without the addition of DMSO or EDTA. Error bars represent the SEM for at least three independent experiments. (C) Lipid peroxidation levels measured in E. coli exposed to dH 2 O, H 2 O 2 , and CB-L with and without the addition of DMSO or EDTA. Values represent the percent change in lipid peroxidation over the water-exposed control, and the SEM for at least three independent experiments. (D) Viability of logarithmic phase E. coli following CB-L exposure with and without the addition of DMSO or EDTA. Error bars represent the SEM for at least three independent experiments. The dotted line represents the limit of detection. *, p

    Techniques Used: Concentration Assay

    Assessment of E. coli ATCC 25922 (A) and MRSA (B) viability following exposure to SMM3 and SMM16 synthetic microbicidal mixtures of metal salts. The dotted line represents the limit of detection.
    Figure Legend Snippet: Assessment of E. coli ATCC 25922 (A) and MRSA (B) viability following exposure to SMM3 and SMM16 synthetic microbicidal mixtures of metal salts. The dotted line represents the limit of detection.

    Techniques Used:

    28) Product Images from "In Vivo Pharmacodynamic Activities of Two Glycylcyclines (GAR-936 and WAY 152,288) against Various Gram-Positive and Gram-Negative Bacteria"

    Article Title: In Vivo Pharmacodynamic Activities of Two Glycylcyclines (GAR-936 and WAY 152,288) against Various Gram-Positive and Gram-Negative Bacteria

    Journal: Antimicrobial Agents and Chemotherapy

    doi:

    In vivo PAE of GAR-936 after administration of 3 mg/kg subcutaneously to neutropenic mice infected with S. pneumoniae ATCC 10813 (a) and E. coli ATCC 25922 (b). t > MIC, period when an active concentration of GAR-936 is present; ●, untreated mice; ○, GAR-936-treated mice.
    Figure Legend Snippet: In vivo PAE of GAR-936 after administration of 3 mg/kg subcutaneously to neutropenic mice infected with S. pneumoniae ATCC 10813 (a) and E. coli ATCC 25922 (b). t > MIC, period when an active concentration of GAR-936 is present; ●, untreated mice; ○, GAR-936-treated mice.

    Techniques Used: In Vivo, Mouse Assay, Infection, Concentration Assay

    29) Product Images from "Induction of antimicrobial peptides secretion by IL-1β enhances human amniotic membrane for regenerative medicine"

    Article Title: Induction of antimicrobial peptides secretion by IL-1β enhances human amniotic membrane for regenerative medicine

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-17210-7

    The inhibition zone of mesenchymal side up and epithelial side up amnion in, ( a ) P. aeruginosa ATCC 27853 culture, ( b ) E. coli T3 culture (The arrows indicate the inhibition zone), ( c ) E. coli T4 culture (The arrows indicate the inhibition zone), ( d ) No inhibitory effect appeared in; E. coli ATCC 25922 (left) and S. aureus ATCC 25923 (right). The experiment was performed in triplicate (n = 5 for each time).
    Figure Legend Snippet: The inhibition zone of mesenchymal side up and epithelial side up amnion in, ( a ) P. aeruginosa ATCC 27853 culture, ( b ) E. coli T3 culture (The arrows indicate the inhibition zone), ( c ) E. coli T4 culture (The arrows indicate the inhibition zone), ( d ) No inhibitory effect appeared in; E. coli ATCC 25922 (left) and S. aureus ATCC 25923 (right). The experiment was performed in triplicate (n = 5 for each time).

    Techniques Used: Inhibition

    30) Product Images from "Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms"

    Article Title: Incorporation of Actinobacillus pleuropneumoniae in Preformed Biofilms by Escherichia coli Isolated From Drinking Water of Swine Farms

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2018.00184

    Scanning electron microscopy images of biofilms formed by A. pleuropneumoniae and E. coli . Mono-species and two-species biofilms constituted by (A) A. pleuropneumoniae (719), (B) E. coli (ATCC 25922) (Magnification: 3500x) and (C–D) A. pleuropneumoniae and E. coli (719 and ATCC 25922 respectively) (Magnification: 5000x). Fimbria- and curli-like structures in biofilms formed by E. coli and E. coli - A. pleuropneumoniae are indicated. (D) shows the sizes of bacteria (white labels); cells were painted to distinguish the two apparent populations of bacteria. Scale bar 5 μm.
    Figure Legend Snippet: Scanning electron microscopy images of biofilms formed by A. pleuropneumoniae and E. coli . Mono-species and two-species biofilms constituted by (A) A. pleuropneumoniae (719), (B) E. coli (ATCC 25922) (Magnification: 3500x) and (C–D) A. pleuropneumoniae and E. coli (719 and ATCC 25922 respectively) (Magnification: 5000x). Fimbria- and curli-like structures in biofilms formed by E. coli and E. coli - A. pleuropneumoniae are indicated. (D) shows the sizes of bacteria (white labels); cells were painted to distinguish the two apparent populations of bacteria. Scale bar 5 μm.

    Techniques Used: Electron Microscopy

    31) Product Images from "Outer Membrane Protein of Gut Commensal Microorganism Induces Autoantibody Production and Extra-Intestinal Gland Inflammation in Mice"

    Article Title: Outer Membrane Protein of Gut Commensal Microorganism Induces Autoantibody Production and Extra-Intestinal Gland Inflammation in Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19103241

    Phylogenetic analysis of the Proteobacteria pylum based on OmpA amino acid sequences by Maximum Likelihood method conducted on MEGA X software (Molecular Evolutionary Genetics Analysis, State College, PA, USA), as described in Reference [ 29 ]. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Genera of Proteobacteria phylum included those of classes Gammaproteobacteria and Betaproteobacteria ( A ). Multiple sequence alignment of OmpA G80 to P305 of E. coli ATCC 25922 with amino acids of Gammaproteobacteria/Enterobacteriales by ClustalW (Available online: https://www.genome.jp/tools-bin/clustalw ). Conserved residues are highlighted with asterisks. Amino acids exhibiting strong and weak similarities according to point-accepted-mutation are indicated by colons and points, respectively. Scores (%) of aligned similarities compared with those of E. coli ATCC 25922 are shown ( B ).
    Figure Legend Snippet: Phylogenetic analysis of the Proteobacteria pylum based on OmpA amino acid sequences by Maximum Likelihood method conducted on MEGA X software (Molecular Evolutionary Genetics Analysis, State College, PA, USA), as described in Reference [ 29 ]. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Genera of Proteobacteria phylum included those of classes Gammaproteobacteria and Betaproteobacteria ( A ). Multiple sequence alignment of OmpA G80 to P305 of E. coli ATCC 25922 with amino acids of Gammaproteobacteria/Enterobacteriales by ClustalW (Available online: https://www.genome.jp/tools-bin/clustalw ). Conserved residues are highlighted with asterisks. Amino acids exhibiting strong and weak similarities according to point-accepted-mutation are indicated by colons and points, respectively. Scores (%) of aligned similarities compared with those of E. coli ATCC 25922 are shown ( B ).

    Techniques Used: Software, Sequencing, Mutagenesis

    32) Product Images from "Outer Membrane Protein of Gut Commensal Microorganism Induces Autoantibody Production and Extra-Intestinal Gland Inflammation in Mice"

    Article Title: Outer Membrane Protein of Gut Commensal Microorganism Induces Autoantibody Production and Extra-Intestinal Gland Inflammation in Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19103241

    Phylogenetic analysis of the Proteobacteria pylum based on OmpA amino acid sequences by Maximum Likelihood method conducted on MEGA X software (Molecular Evolutionary Genetics Analysis, State College, PA, USA), as described in Reference [ 29 ]. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Genera of Proteobacteria phylum included those of classes Gammaproteobacteria and Betaproteobacteria ( A ). Multiple sequence alignment of OmpA G80 to P305 of E. coli ATCC 25922 with amino acids of Gammaproteobacteria/Enterobacteriales by ClustalW (Available online: https://www.genome.jp/tools-bin/clustalw ). Conserved residues are highlighted with asterisks. Amino acids exhibiting strong and weak similarities according to point-accepted-mutation are indicated by colons and points, respectively. Scores (%) of aligned similarities compared with those of E. coli ATCC 25922 are shown ( B ).
    Figure Legend Snippet: Phylogenetic analysis of the Proteobacteria pylum based on OmpA amino acid sequences by Maximum Likelihood method conducted on MEGA X software (Molecular Evolutionary Genetics Analysis, State College, PA, USA), as described in Reference [ 29 ]. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Genera of Proteobacteria phylum included those of classes Gammaproteobacteria and Betaproteobacteria ( A ). Multiple sequence alignment of OmpA G80 to P305 of E. coli ATCC 25922 with amino acids of Gammaproteobacteria/Enterobacteriales by ClustalW (Available online: https://www.genome.jp/tools-bin/clustalw ). Conserved residues are highlighted with asterisks. Amino acids exhibiting strong and weak similarities according to point-accepted-mutation are indicated by colons and points, respectively. Scores (%) of aligned similarities compared with those of E. coli ATCC 25922 are shown ( B ).

    Techniques Used: Software, Sequencing, Mutagenesis

    33) Product Images from "In Vitro Effect of qnrA1, qnrB1, and qnrS1 Genes on Fluoroquinolone Activity against Isogenic Escherichia coli Isolates with Mutations in gyrA and parC ▿"

    Article Title: In Vitro Effect of qnrA1, qnrB1, and qnrS1 Genes on Fluoroquinolone Activity against Isogenic Escherichia coli Isolates with Mutations in gyrA and parC ▿

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00927-10

    Viable bacterial counts in time-kill curve assays with ciprofloxacin (CIP) 1 μg/ml. (A) Isogenic wild-type  E. coli  ATCC 25922, with and without  qnrA1 ,  qnrB1 , or  qnrS1  gene expression; (B) isogenic mutant  E. coli  ATCC 25922-S83L, with and without  qnrA1 ,  qnrB1 , or  qnrS1  gene expression.
    Figure Legend Snippet: Viable bacterial counts in time-kill curve assays with ciprofloxacin (CIP) 1 μg/ml. (A) Isogenic wild-type E. coli ATCC 25922, with and without qnrA1 , qnrB1 , or qnrS1 gene expression; (B) isogenic mutant E. coli ATCC 25922-S83L, with and without qnrA1 , qnrB1 , or qnrS1 gene expression.

    Techniques Used: Expressing, Mutagenesis

    34) Product Images from "Study of Benzofuroquinolinium Derivatives as a New Class of Potent Antibacterial Agent and the Mode of Inhibition Targeting FtsZ"

    Article Title: Study of Benzofuroquinolinium Derivatives as a New Class of Potent Antibacterial Agent and the Mode of Inhibition Targeting FtsZ

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.01937

    Time Killing Curve of 5 against E. coli ATCC 25922 (A) and S. aureus ATCC 29213 (B) . The different concentration of 5 was represented by different colors, 0× MIC (1% DMSO) (black), 1× MIC (red), 2× MIC (blue), 4× MIC (pink) and 8× MIC (green).
    Figure Legend Snippet: Time Killing Curve of 5 against E. coli ATCC 25922 (A) and S. aureus ATCC 29213 (B) . The different concentration of 5 was represented by different colors, 0× MIC (1% DMSO) (black), 1× MIC (red), 2× MIC (blue), 4× MIC (pink) and 8× MIC (green).

    Techniques Used: Concentration Assay

    35) Product Images from "Study of pandrug and heavy metal resistance among E. coli from anthropogenically influenced Delhi stretch of river Yamuna"

    Article Title: Study of pandrug and heavy metal resistance among E. coli from anthropogenically influenced Delhi stretch of river Yamuna

    Journal: Brazilian Journal of Microbiology

    doi: 10.1016/j.bjm.2017.11.001

    Growth pattern of bacteria under different culture conditions. (A) Isolate  E. coli  MRC11, (B) isolate  E. coli  J53 trans-conjugant, (C) positive control (i.e., isolate resistant to β-lactams and heavy metals) and (D) sensitive control  E. coli  ATCC 25922. The  X  axis depicts time in hours where as  Y  axis represents bacterial growth presented in terms of OD at 600 nm.
    Figure Legend Snippet: Growth pattern of bacteria under different culture conditions. (A) Isolate E. coli MRC11, (B) isolate E. coli J53 trans-conjugant, (C) positive control (i.e., isolate resistant to β-lactams and heavy metals) and (D) sensitive control E. coli ATCC 25922. The X axis depicts time in hours where as Y axis represents bacterial growth presented in terms of OD at 600 nm.

    Techniques Used: Positive Control

    36) Product Images from "Cellular Response to Ciprofloxacin in Low-Level Quinolone-Resistant Escherichia coli"

    Article Title: Cellular Response to Ciprofloxacin in Low-Level Quinolone-Resistant Escherichia coli

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01370

    Impact on SOS response of low-level quinolone-resistant (LLQR) cells exposed to 1 μg/mL of ciprofloxacin, compared to wild-type cells in the same conditions. LLQR phenotypes: EC14 means E. coli ATCC 25922 pBK-QnrS1; EC19 means E. coli ATCC 25922 ΔmarR pBK-QnrS1; and EC24 means E. coli ATCC 25922 S83L pBK-QnrS1. All indicated genes shown a pattern of expression significantly different among LLQR strains and wild-type E. coli ( p -value
    Figure Legend Snippet: Impact on SOS response of low-level quinolone-resistant (LLQR) cells exposed to 1 μg/mL of ciprofloxacin, compared to wild-type cells in the same conditions. LLQR phenotypes: EC14 means E. coli ATCC 25922 pBK-QnrS1; EC19 means E. coli ATCC 25922 ΔmarR pBK-QnrS1; and EC24 means E. coli ATCC 25922 S83L pBK-QnrS1. All indicated genes shown a pattern of expression significantly different among LLQR strains and wild-type E. coli ( p -value

    Techniques Used: Expressing

    Impact on ROS response of low-level quinolone-resistant (LLQR) cells exposed to 1 μg/mL of ciprofloxacin compared to wild-type cells in the same conditions. LLQR phenotypes: EC14 means E. coli ATCC 25922 pBK-QnrS1; EC19 means E. coli ATCC 25922 ΔmarR pBK-QnrS1; and EC24 means E. coli ATCC 25922 S83L pBK-QnrS1. All indicated genes show a significantly different pattern of expression between LLQR strains and wild-type E. coli ( p -value
    Figure Legend Snippet: Impact on ROS response of low-level quinolone-resistant (LLQR) cells exposed to 1 μg/mL of ciprofloxacin compared to wild-type cells in the same conditions. LLQR phenotypes: EC14 means E. coli ATCC 25922 pBK-QnrS1; EC19 means E. coli ATCC 25922 ΔmarR pBK-QnrS1; and EC24 means E. coli ATCC 25922 S83L pBK-QnrS1. All indicated genes show a significantly different pattern of expression between LLQR strains and wild-type E. coli ( p -value

    Techniques Used: Expressing

    37) Product Images from "Human Defensin 5 Disulfide Array Mutants: Disulfide Bond Deletion Attenuates Antibacterial Activity Against Staphylococcus aureus"

    Article Title: Human Defensin 5 Disulfide Array Mutants: Disulfide Bond Deletion Attenuates Antibacterial Activity Against Staphylococcus aureus

    Journal: Biochemistry

    doi: 10.1021/bi201043j

    Antibacterial activity assays with the HD5 ox  mutant family (CFU method). 1, HD5 ox  ] ox  ] ox  ] ox  ] ox  ] ox  ] ox  (3-20)(5-31). (AActivity of HD5 ox  and the Ser mutants against  E. coli  ATCC 25922. (B) Activity of HD5 ox  and the Ser mutants against  S. aureus .
    Figure Legend Snippet: Antibacterial activity assays with the HD5 ox mutant family (CFU method). 1, HD5 ox ] ox ] ox ] ox ] ox ] ox ] ox (3-20)(5-31). (AActivity of HD5 ox and the Ser mutants against E. coli ATCC 25922. (B) Activity of HD5 ox and the Ser mutants against S. aureus .

    Techniques Used: Activity Assay, Mutagenesis

    38) Product Images from "Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential"

    Article Title: Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00835-17

    MCR-1 detection by the modified rapid polymyxin NP test (MPNP). Modification of the NP test was based on incorporation of two additional wells, which were filled with colistin-free solution plus EDTA (80 μg/ml) and colistin-containing (5 μg/ml) solution plus EDTA, respectively. Wells A1 to A7 were filled with 150 μl of a colistin-free NP solution. Wells B1 to B7 were completed with 150 μl of NP solution supplemented with 5 μg/ml colistin  sulfate. Wells C1 to C7 were filled with 150 μl of colistin-free NP solution supplemented with 80 μg/ml EDTA. Wells D1 to D7 were added with 150 μl of NP solution containing 5 μg/ml colistin sulfate and 80 μg/ml EDTA. Wells in column 1 were filled with 50 μl of 0.85% NaCl (negative sterility control), whereas for each isolate, 50 μl of a 3.0 to 3.5 McFarland bacterial suspension (∼10 9  UFC/ml) was dispensed and mixed with 150 μl of reaction solution contained in each of the wells in columns 2 to 7. Columns 2 to 7 represent the MPNP test performed for  E. coli  ATCC 25922,  P. mirabilis  ATCC 25933, colistin-resistant (Col-R)  K. pneumoniae  Alerta 16 ( mgrB  promoter IS 1  family), colistin-resistant  mcr-1 -negative  E. coli  strain HC113, colistin-resistant  mcr-1 -negative  E. coli  Δ806 mutant strain, and  mcr-1 -positive  E. coli  strain ICBEC72H, respectively. The plates were incubated at 35 ± 2°C under aerobic conditions for 4 h, and visual changes in the color of the wells were monitored each hour. In wells with added colistin (B1 t oB7), a color change from orange to yellow was considered positive to colistin resistance, whereas the MPNP test was considered positive to MCR-1 phosphoethanolamine transferase production when the colistin-containing solution supplemented with EDTA (wells D1 to D7) remained orange (i.e., absence of glucose metabolization); this shows that growth of the colistin-resistant  E. coli  ( mcr-1 -positive) in the well containing colistin solution (well D7) was inhibited by EDTA.
    Figure Legend Snippet: MCR-1 detection by the modified rapid polymyxin NP test (MPNP). Modification of the NP test was based on incorporation of two additional wells, which were filled with colistin-free solution plus EDTA (80 μg/ml) and colistin-containing (5 μg/ml) solution plus EDTA, respectively. Wells A1 to A7 were filled with 150 μl of a colistin-free NP solution. Wells B1 to B7 were completed with 150 μl of NP solution supplemented with 5 μg/ml colistin sulfate. Wells C1 to C7 were filled with 150 μl of colistin-free NP solution supplemented with 80 μg/ml EDTA. Wells D1 to D7 were added with 150 μl of NP solution containing 5 μg/ml colistin sulfate and 80 μg/ml EDTA. Wells in column 1 were filled with 50 μl of 0.85% NaCl (negative sterility control), whereas for each isolate, 50 μl of a 3.0 to 3.5 McFarland bacterial suspension (∼10 9 UFC/ml) was dispensed and mixed with 150 μl of reaction solution contained in each of the wells in columns 2 to 7. Columns 2 to 7 represent the MPNP test performed for E. coli ATCC 25922, P. mirabilis ATCC 25933, colistin-resistant (Col-R) K. pneumoniae Alerta 16 ( mgrB promoter IS 1 family), colistin-resistant mcr-1 -negative E. coli strain HC113, colistin-resistant mcr-1 -negative E. coli Δ806 mutant strain, and mcr-1 -positive E. coli strain ICBEC72H, respectively. The plates were incubated at 35 ± 2°C under aerobic conditions for 4 h, and visual changes in the color of the wells were monitored each hour. In wells with added colistin (B1 t oB7), a color change from orange to yellow was considered positive to colistin resistance, whereas the MPNP test was considered positive to MCR-1 phosphoethanolamine transferase production when the colistin-containing solution supplemented with EDTA (wells D1 to D7) remained orange (i.e., absence of glucose metabolization); this shows that growth of the colistin-resistant E. coli ( mcr-1 -positive) in the well containing colistin solution (well D7) was inhibited by EDTA.

    Techniques Used: Modification, Sterility, Mutagenesis, Incubation

    39) Product Images from "Enhancing the Antibacterial Activity of Light-Activated Surfaces Containing Crystal Violet and ZnO Nanoparticles: Investigation of Nanoparticle Size, Capping Ligand, and Dopants"

    Article Title: Enhancing the Antibacterial Activity of Light-Activated Surfaces Containing Crystal Violet and ZnO Nanoparticles: Investigation of Nanoparticle Size, Capping Ligand, and Dopants

    Journal: ACS Omega

    doi: 10.1021/acsomega.6b00017

    Viable counts of E. coli ATCC 25922 after incubation at 20 °C on modified polyurethane squares for (a) 4 h in the dark and (b) 2 h with exposure to white-light illumination with an average light intensity of 6600 ± 900 lx at a distance of 25 cm from the samples. *Bacterial counts were reduced to below the detection limit of 100 cfu/mL. OA indicates NPs synthesized with OA capping. Error bars are based on the standard deviation of three experimental replicates.
    Figure Legend Snippet: Viable counts of E. coli ATCC 25922 after incubation at 20 °C on modified polyurethane squares for (a) 4 h in the dark and (b) 2 h with exposure to white-light illumination with an average light intensity of 6600 ± 900 lx at a distance of 25 cm from the samples. *Bacterial counts were reduced to below the detection limit of 100 cfu/mL. OA indicates NPs synthesized with OA capping. Error bars are based on the standard deviation of three experimental replicates.

    Techniques Used: Incubation, Modification, Synthesized, Standard Deviation

    Viable counts of E. coli ATCC 25922 after incubation at 20 °C on modified polyurethane squares for (a) 2 h in the dark, (b) 2 h with exposure to standard laboratory white light (500 ± 300 lx), (c) 3 h in the dark, and (d) 3 h with exposure to standard laboratory white light (500 ± 300 lx). *Bacterial counts were reduced to below the detection limit of 100 cfu/mL. DOPA indicates NPs synthesized with DOPA capping. Error bars are based on the standard deviations of three experimental replicates.
    Figure Legend Snippet: Viable counts of E. coli ATCC 25922 after incubation at 20 °C on modified polyurethane squares for (a) 2 h in the dark, (b) 2 h with exposure to standard laboratory white light (500 ± 300 lx), (c) 3 h in the dark, and (d) 3 h with exposure to standard laboratory white light (500 ± 300 lx). *Bacterial counts were reduced to below the detection limit of 100 cfu/mL. DOPA indicates NPs synthesized with DOPA capping. Error bars are based on the standard deviations of three experimental replicates.

    Techniques Used: Incubation, Modification, Synthesized

    40) Product Images from "Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis"

    Article Title: Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-01278-2

    Consistent AST results from a wide range of inoculum sizes. ( A ) Colony formation of bacteria at different inoculum sizes. In all inoculum sizes from 5.0 × 10 7 to 5.0 × 10 5 CFU/ml, there was microcolony formation at 0, 0.25 and 0.5 μg/ml gentamicin. At 1 μg/ml gentamicin, there was no colony formation at any inoculum size. The scale bar represents 100 μm. ( B ) MIC values from different E. coli ATCC 25922 inoculum sizes of 5.0 × 10 7 , 5.0 × 10 6 and 5.0 × 10 5 CFU/ml incubated with 17 clinically important antimicrobials and analyzed using dRAST and broth microdilution test. For all inoculum sizes, the MIC values were in the middle of the CLSI quality control ranges. (C) Summary of a comparison of AST results from various inoculum sizes of E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. aureus ATCC 29213 and E. faecalis ATCC 29212. “Correct” means a case with identical MIC values from different inoculum sizes. “One two-fold” and “Two two-fold” denote cases with MIC values from different inoculum sizes that were different by two-fold and four-fold, respectively. “Essential agreement” refers to the proportion of cases that were correct or had one two-fold difference. The essential agreement rates were 99.33% in E. coli ATCC 25922, 100% in P. aeruginosa ATCC 27853, 95.37% in S. aureus ATCC 29213 and 100% in E. faecalis ATCC 29212.
    Figure Legend Snippet: Consistent AST results from a wide range of inoculum sizes. ( A ) Colony formation of bacteria at different inoculum sizes. In all inoculum sizes from 5.0 × 10 7 to 5.0 × 10 5 CFU/ml, there was microcolony formation at 0, 0.25 and 0.5 μg/ml gentamicin. At 1 μg/ml gentamicin, there was no colony formation at any inoculum size. The scale bar represents 100 μm. ( B ) MIC values from different E. coli ATCC 25922 inoculum sizes of 5.0 × 10 7 , 5.0 × 10 6 and 5.0 × 10 5 CFU/ml incubated with 17 clinically important antimicrobials and analyzed using dRAST and broth microdilution test. For all inoculum sizes, the MIC values were in the middle of the CLSI quality control ranges. (C) Summary of a comparison of AST results from various inoculum sizes of E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. aureus ATCC 29213 and E. faecalis ATCC 29212. “Correct” means a case with identical MIC values from different inoculum sizes. “One two-fold” and “Two two-fold” denote cases with MIC values from different inoculum sizes that were different by two-fold and four-fold, respectively. “Essential agreement” refers to the proportion of cases that were correct or had one two-fold difference. The essential agreement rates were 99.33% in E. coli ATCC 25922, 100% in P. aeruginosa ATCC 27853, 95.37% in S. aureus ATCC 29213 and 100% in E. faecalis ATCC 29212.

    Techniques Used: AST Assay, Incubation

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    Article Snippet: .. The antibacterial function of the AMPs was evaluated in vitro using antibacterial assays, and in vivo after their administration to BALB/c mice infected with Escherichia coli ( E. coli , ATCC 25922). .. We also investigated the immunomodulatory activities of the recombinant hybrid antimicrobial peptide and C. militaris producing AMPs, and provide here preliminary scientific evidence for the future production of C. militaris mycelium producing AMPs as potential feed additives for livestock.

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    ATCC cefiderocol
    <t>Cefiderocol</t> MIC distributions for all isolates of MDR P. aeruginosa (white bars; n = 262), isolates of MDR P. aeruginosa that were concurrently nonsusceptible to ceftolozane-tazobactam (gray bars; n = 199), and isolates of MDR P. aeruginosa that were concurrently nonsusceptible to ceftazidime-avibactam (black bars; n = 167).
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    Cefiderocol MIC distributions for all isolates of MDR P. aeruginosa (white bars; n = 262), isolates of MDR P. aeruginosa that were concurrently nonsusceptible to ceftolozane-tazobactam (gray bars; n = 199), and isolates of MDR P. aeruginosa that were concurrently nonsusceptible to ceftazidime-avibactam (black bars; n = 167).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vitro Activity of the Siderophore Cephalosporin, Cefiderocol, against Carbapenem-Nonsusceptible and Multidrug-Resistant Isolates of Gram-Negative Bacilli Collected Worldwide in 2014 to 2016

    doi: 10.1128/AAC.01968-17

    Figure Lengend Snippet: Cefiderocol MIC distributions for all isolates of MDR P. aeruginosa (white bars; n = 262), isolates of MDR P. aeruginosa that were concurrently nonsusceptible to ceftolozane-tazobactam (gray bars; n = 199), and isolates of MDR P. aeruginosa that were concurrently nonsusceptible to ceftazidime-avibactam (black bars; n = 167).

    Article Snippet: All quality control results were within specified CLSI ranges ( ) including CLSI approved, but not yet published, ranges for cefiderocol ( E. coli ATCC 25922, 0.06 to 0.5 μg/ml; P. aeruginosa ATCC 27853, 0.06 to 0.5 μg/ml) ( , , ).

    Techniques:

    Cumulative cefiderocol MIC distribution (percentage of isolates) for 262 isolates of MDR P. aeruginosa .

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vitro Activity of the Siderophore Cephalosporin, Cefiderocol, against Carbapenem-Nonsusceptible and Multidrug-Resistant Isolates of Gram-Negative Bacilli Collected Worldwide in 2014 to 2016

    doi: 10.1128/AAC.01968-17

    Figure Lengend Snippet: Cumulative cefiderocol MIC distribution (percentage of isolates) for 262 isolates of MDR P. aeruginosa .

    Article Snippet: All quality control results were within specified CLSI ranges ( ) including CLSI approved, but not yet published, ranges for cefiderocol ( E. coli ATCC 25922, 0.06 to 0.5 μg/ml; P. aeruginosa ATCC 27853, 0.06 to 0.5 μg/ml) ( , , ).

    Techniques:

    Cumulative cefiderocol MIC distribution (percentage of isolates) for 1,022 isolates of meropenem-nonsusceptible Enterobacteriaceae .

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vitro Activity of the Siderophore Cephalosporin, Cefiderocol, against Carbapenem-Nonsusceptible and Multidrug-Resistant Isolates of Gram-Negative Bacilli Collected Worldwide in 2014 to 2016

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    Figure Lengend Snippet: Cumulative cefiderocol MIC distribution (percentage of isolates) for 1,022 isolates of meropenem-nonsusceptible Enterobacteriaceae .

    Article Snippet: All quality control results were within specified CLSI ranges ( ) including CLSI approved, but not yet published, ranges for cefiderocol ( E. coli ATCC 25922, 0.06 to 0.5 μg/ml; P. aeruginosa ATCC 27853, 0.06 to 0.5 μg/ml) ( , , ).

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    Cumulative cefiderocol MIC distribution (percentage of isolates) for 217 isolates of S. maltophilia .

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    Figure Lengend Snippet: Cumulative cefiderocol MIC distribution (percentage of isolates) for 217 isolates of S. maltophilia .

    Article Snippet: All quality control results were within specified CLSI ranges ( ) including CLSI approved, but not yet published, ranges for cefiderocol ( E. coli ATCC 25922, 0.06 to 0.5 μg/ml; P. aeruginosa ATCC 27853, 0.06 to 0.5 μg/ml) ( , , ).

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    Antibacterial activities of Fr2 of green tea seed saponins against Escherichia coli, Salmonella typhimurium, Salmonella enteritidis, Salmonella gallinarum, Salmonella choleraesuis, Salmonella pullorum, Salmonella dublin, and Staphylococcus aureus . The antibacterial activities were determined by allowing the bacteria to grow in the presence of various concentrations of saponins using 96-well microtiter plate and then checking the viability of bacteria by measuring OD 660 with spectrophotometer. Data are expressed as means of experiments in triplicate ± SEM. Data are statistically significant at P

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    Figure Lengend Snippet: Antibacterial activities of Fr2 of green tea seed saponins against Escherichia coli, Salmonella typhimurium, Salmonella enteritidis, Salmonella gallinarum, Salmonella choleraesuis, Salmonella pullorum, Salmonella dublin, and Staphylococcus aureus . The antibacterial activities were determined by allowing the bacteria to grow in the presence of various concentrations of saponins using 96-well microtiter plate and then checking the viability of bacteria by measuring OD 660 with spectrophotometer. Data are expressed as means of experiments in triplicate ± SEM. Data are statistically significant at P

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    Antibacterial activities of Fr1 of green tea seed saponins against Escherichia coli, Salmonella typhimurium, Salmonella enteritidis, Salmonella gallinarum, Salmonella choleraesuis, Salmonella pullorum, Salmonella dublin, and Staphylococcus aureus . The antibacterial activities were determined by allowing the bacteria to grow in the presence of various concentrations of saponins using 96-well microtiter plate and then checking the viability of bacteria by measuring OD 660 with spectrophotometer. Data are expressed as means of experiments in triplicate ± SEM. Data are statistically significant at P

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    doi: 10.1155/2018/3486106

    Figure Lengend Snippet: Antibacterial activities of Fr1 of green tea seed saponins against Escherichia coli, Salmonella typhimurium, Salmonella enteritidis, Salmonella gallinarum, Salmonella choleraesuis, Salmonella pullorum, Salmonella dublin, and Staphylococcus aureus . The antibacterial activities were determined by allowing the bacteria to grow in the presence of various concentrations of saponins using 96-well microtiter plate and then checking the viability of bacteria by measuring OD 660 with spectrophotometer. Data are expressed as means of experiments in triplicate ± SEM. Data are statistically significant at P

    Article Snippet: The strains used were Escherichia coli (ATCC 25922), Salmonella typhimurium (ATCC 14028), Salmonella enteritidis (ATCC 13076), Salmonella gallinarum (ATCC 9184), Salmonella choleraesuis (ATCC 7001), Salmonella pullorum (ATCC 19945), Salmonella dublin (ATCC 15480), and Staphylococcus aureus (ATCC 12600).

    Techniques: Spectrophotometry

    Plate counting photographs of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus co-cultured with Ag-C and Ag or with 10× NaCl in vitro for 3 h ( A – J ). Notes: The corresponding inhibition rates of bacteria ( K ). ** p

    Journal: International Journal of Nanomedicine

    Article Title: NaCl: for the safer in vivo use of antibacterial silver based nanoparticles

    doi: 10.2147/IJN.S153168

    Figure Lengend Snippet: Plate counting photographs of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus co-cultured with Ag-C and Ag or with 10× NaCl in vitro for 3 h ( A – J ). Notes: The corresponding inhibition rates of bacteria ( K ). ** p

    Article Snippet: Staphylococcus aureus (American Type Culture Collection, Manassas, VA, USA [ATCC] 25923), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 10145) were purchased from ATCC.

    Techniques: Cell Culture, In Vitro, Inhibition

    The effects of Mag II-CB and CB treatment in the mice, as determined by intestinal histology. Representative images for the different groups are shown at ×40 magnification. a Normal control (NC); b recombinant Mag II-CB (MC); c recombinant CB (CB); d Escherichia coli (ATCC 25922) (EI); e E. coli -infected mice treated with Mag II-CB (MC + EI); f E. coli -infected mice treated with CB (CB + EI)

    Journal: Microbial Cell Factories

    Article Title: Expression of a recombinant hybrid antimicrobial peptide magainin II-cecropin B in the mycelium of the medicinal fungus Cordyceps militaris and its validation in mice

    doi: 10.1186/s12934-018-0865-3

    Figure Lengend Snippet: The effects of Mag II-CB and CB treatment in the mice, as determined by intestinal histology. Representative images for the different groups are shown at ×40 magnification. a Normal control (NC); b recombinant Mag II-CB (MC); c recombinant CB (CB); d Escherichia coli (ATCC 25922) (EI); e E. coli -infected mice treated with Mag II-CB (MC + EI); f E. coli -infected mice treated with CB (CB + EI)

    Article Snippet: The antibacterial function of the AMPs was evaluated in vitro using antibacterial assays, and in vivo after their administration to BALB/c mice infected with Escherichia coli ( E. coli , ATCC 25922).

    Techniques: Mouse Assay, Recombinant, Infection