anti dynein ic1 2  (Santa Cruz Biotechnology)

 
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    Santa Cruz Biotechnology anti dynein ic1 2
    Anti Dynein Ic1 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    • anti dynein  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology anti dynein
    NPs synthesis and characterization (A) NP-complex synthesis process entails several consecutive steps, where at each step, a single component is added. (B) Mean anchoring distance between neighboring PEG-NLSs, ξ*, against the concentration of NLS. The grey dots correspond to extrapolated values which were calculated from the fit of 〈N〉 vs. [NLS] (see Table 1 , SI.1 and Fig. S1.2 ). Error bars indicate the standard deviations for 3 experiments. (C) Western blot (WB) analysis results demonstrating the recruitment of <t>importin-α2</t> (a) and mammalian <t>Dynein</t> motors (b) to the NPs after incubation in Hela cells extract. Group 1 (in both (a) and (b)) refers to Hela cells extract without NPs, group 2 refers to NPs coated with Biotin-PEG-SH, and group 3 refers to PEG-NLS coated NPs. The concentration of cells extract used in WB is 3.4 mg/ml.
    Anti Dynein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti dynein/product/Santa Cruz Biotechnology
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti dynein - by Bioz Stars, 2022-10
    95/100 stars
    		  Buy from Supplier

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    NPs synthesis and characterization (A) NP-complex synthesis process entails several consecutive steps, where at each step, a single component is added. (B) Mean anchoring distance between neighboring PEG-NLSs, ξ*, against the concentration of NLS. The grey dots correspond to extrapolated values which were calculated from the fit of 〈N〉 vs. [NLS] (see Table 1 , SI.1 and Fig. S1.2 ). Error bars indicate the standard deviations for 3 experiments. (C) Western blot (WB) analysis results demonstrating the recruitment of importin-α2 (a) and mammalian Dynein motors (b) to the NPs after incubation in Hela cells extract. Group 1 (in both (a) and (b)) refers to Hela cells extract without NPs, group 2 refers to NPs coated with Biotin-PEG-SH, and group 3 refers to PEG-NLS coated NPs. The concentration of cells extract used in WB is 3.4 mg/ml.

    Journal: bioRxiv

    Article Title: Nano-particles carried by multiple dynein motors: A Self-Regulating Nano-Machine

    doi: 10.1101/2020.07.09.194720

    Figure Lengend Snippet: NPs synthesis and characterization (A) NP-complex synthesis process entails several consecutive steps, where at each step, a single component is added. (B) Mean anchoring distance between neighboring PEG-NLSs, ξ*, against the concentration of NLS. The grey dots correspond to extrapolated values which were calculated from the fit of 〈N〉 vs. [NLS] (see Table 1 , SI.1 and Fig. S1.2 ). Error bars indicate the standard deviations for 3 experiments. (C) Western blot (WB) analysis results demonstrating the recruitment of importin-α2 (a) and mammalian Dynein motors (b) to the NPs after incubation in Hela cells extract. Group 1 (in both (a) and (b)) refers to Hela cells extract without NPs, group 2 refers to NPs coated with Biotin-PEG-SH, and group 3 refers to PEG-NLS coated NPs. The concentration of cells extract used in WB is 3.4 mg/ml.

    Article Snippet: The membrane was washed 3 times with PBST for 5 min, and then incubated for 1h at 25°C with anti-Dynein (Santa Cruz, sc-13524), anti-Karyoprotein α2 (Santa Cruz, sc-55538), or anti-Karyoprotein β1 antibody (Santa Cruz, sc-137016).

    Techniques: Concentration Assay, Western Blot, Incubation

    Tax expression in MT-2 cells. (A) Flow cytometry analysis of MT-2 cell culture, showing about 50% of Tax expressing cells (anti-Tax APC diluted 1:100). (B) Western blot of MT-2 or K562 cell fractionation into cytosolic (lanes 1), intermediate (lanes 2), and nuclear (lanes 3) fractions. The figure shows western blots using antibodies against Tax (Covalab, diluted 1:1,000), GAPDH (cytosolic marker, diluted 1:20,000), calreticulin (CRT) ER marker, diluted 1:5,000), and TFIIB (nuclear marker, diluted 1:1,000).

    Journal: AIDS Research and Human Retroviruses

    Article Title: Tax Posttranslational Modifications and Interaction with Calreticulin in MT-2 Cells and Human Peripheral Blood Mononuclear Cells of Human T Cell Lymphotropic Virus Type-I-Associated Myelopathy/Tropical Spastic Paraparesis Patients

    doi: 10.1089/aid.2013.0036

    Figure Lengend Snippet: Tax expression in MT-2 cells. (A) Flow cytometry analysis of MT-2 cell culture, showing about 50% of Tax expressing cells (anti-Tax APC diluted 1:100). (B) Western blot of MT-2 or K562 cell fractionation into cytosolic (lanes 1), intermediate (lanes 2), and nuclear (lanes 3) fractions. The figure shows western blots using antibodies against Tax (Covalab, diluted 1:1,000), GAPDH (cytosolic marker, diluted 1:20,000), calreticulin (CRT) ER marker, diluted 1:5,000), and TFIIB (nuclear marker, diluted 1:1,000).

    Article Snippet: Cat. mab0022, diluted 1:1,000) and HTLV-1 Tax hybridoma 168A51-2 (NIH, AIDS Reagent Program, Germantown, MD; Cat. 1316, used as ascitic fluid, diluted 1:1,000), antibodies against ubiquitin (Upstate, Lake Placid, NY; Cat. 04-263, diluted 1:1,000), SUMO-1 (Upstate; Cat. 04-453, diluted 1:1,000), SUMO-2/3 (Abcam Inc., Cambridge, MA; Cat. Ab3742, diluted 1:1,000), GAPDH (Sigma-Aldrich Inc.; Cat. G8795, diluted 1:20,000), TFIIB (Santa Cruz Biotechnology Inc., Santa Cruz, CA; Cat. sc-225, diluted 1:1,000), Dynein IC1/2, cytosolic (Santa Cruz Biotechnology Inc.; Cat. sc-13524, diluted 1:500), MMP-9 (Millipore Merck, Billerica, MA; Cat. MAB3309, diluted 1:500), and polyclonal antibodies against CRT prepared in Dr. Arturo Ferreira's laboratory (diluted 1:5,000 or 1:800,000 according to the antibody titer).

    Techniques: Expressing, Flow Cytometry, Cytometry, Cell Culture, Western Blot, Cell Fractionation, Marker

    Working model of DIAPH3 function in aNPC. ( A ) During mitosis, DIAPH3 maintains the dynamics of cytoskeleton. Polarity proteins NUMA and GPSM2 assemble underneath cortical F-actin and recruit the dynein/dynactin motor protein complex. Dynein and dynactin interact with SPAG5/KNSTRN/CLASP1 located at the microtubule plus-end and attach astral microtubules to cell cortex, thus providing the pulling force for chromosome bipolar segregation ( Okumura et al., 2018 , Dunsch et al., 2011 , Kern et al., 2016 ). ( B ) The position of polarity proteins NUMA/GPSM2 directs the orientation of the mitotic spindle and determines the type of division (proliferative versus neurogenic) of aNPC. ( C , D ) Absence of DIAPH3 destabilizes actin and microtubules and disrupts the expression of SPAG5, KNSTRN, GPSM2, PAR3 and NUMB, therefore weakening astral microtubules-cell cortex and spindle microtubules-kinetochore interactions. This causes spindle abnormalities, chromosome mis-alignment and mis-segregation, and alters fate decision of aNPC.

    Journal: bioRxiv

    Article Title: DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction

    doi: 10.1101/2020.08.11.245829

    Figure Lengend Snippet: Working model of DIAPH3 function in aNPC. ( A ) During mitosis, DIAPH3 maintains the dynamics of cytoskeleton. Polarity proteins NUMA and GPSM2 assemble underneath cortical F-actin and recruit the dynein/dynactin motor protein complex. Dynein and dynactin interact with SPAG5/KNSTRN/CLASP1 located at the microtubule plus-end and attach astral microtubules to cell cortex, thus providing the pulling force for chromosome bipolar segregation ( Okumura et al., 2018 , Dunsch et al., 2011 , Kern et al., 2016 ). ( B ) The position of polarity proteins NUMA/GPSM2 directs the orientation of the mitotic spindle and determines the type of division (proliferative versus neurogenic) of aNPC. ( C , D ) Absence of DIAPH3 destabilizes actin and microtubules and disrupts the expression of SPAG5, KNSTRN, GPSM2, PAR3 and NUMB, therefore weakening astral microtubules-cell cortex and spindle microtubules-kinetochore interactions. This causes spindle abnormalities, chromosome mis-alignment and mis-segregation, and alters fate decision of aNPC.

    Article Snippet: Membranes were blocked with StartingBlock buffer (ThermoScientific) and incubated overnight at 4°C with rabbit anti-DIAPH3 (1:5000) , rabbit anti-SPAG5 (Sigma, HPA022008, 1:750;), chicken anti-GAPDH (Millipore, AB2302, 1:2000), mouse anti-NUMA (Becton Dickinson, 610561, 1:500), rabbit anti-PAR3 (Millipore, 07-330, 1 :500), rabbit anti-GPSM2 (1 :200) , rabbit anti-CENPA (Cell Signaling, 2048, 1 :500), mouse anti-dynein (Santa Cruz, sc-13524, 1 :500), rabbit anti-KNSTRN (Sigma, HPA042027, 1:1000), rabbit anti-INSC (Abcam, ab102953, 1:1000), goat anti-NUMB (Abcam, ab4147, 1:100), mouse anti-dynactin (BD Biosciences, 610474, 1:500), rabbit anti-CLASP1 (Abcam, ab108620, 1:5000).

    Techniques: Expressing

    Diaph3-deficiency impairs the expression and stability of mitotic spindle polarity proteins. ( A, B ) Assessment of NUMA, GPSM2, INSC, NUMB, PAR3, Dynein, Dynactin, SPAG5, KNSTRN, and CLASP1 levels in telencephalon extracts of Diaph3 KO mice by western blotting. CENPA was used as positive control ( Liu and Mao, 2016 ) and GAPDH as loading control for quantification ( n =3 embryos for each genotype. *: P

    Journal: bioRxiv

    Article Title: DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction

    doi: 10.1101/2020.08.11.245829

    Figure Lengend Snippet: Diaph3-deficiency impairs the expression and stability of mitotic spindle polarity proteins. ( A, B ) Assessment of NUMA, GPSM2, INSC, NUMB, PAR3, Dynein, Dynactin, SPAG5, KNSTRN, and CLASP1 levels in telencephalon extracts of Diaph3 KO mice by western blotting. CENPA was used as positive control ( Liu and Mao, 2016 ) and GAPDH as loading control for quantification ( n =3 embryos for each genotype. *: P

    Article Snippet: Membranes were blocked with StartingBlock buffer (ThermoScientific) and incubated overnight at 4°C with rabbit anti-DIAPH3 (1:5000) , rabbit anti-SPAG5 (Sigma, HPA022008, 1:750;), chicken anti-GAPDH (Millipore, AB2302, 1:2000), mouse anti-NUMA (Becton Dickinson, 610561, 1:500), rabbit anti-PAR3 (Millipore, 07-330, 1 :500), rabbit anti-GPSM2 (1 :200) , rabbit anti-CENPA (Cell Signaling, 2048, 1 :500), mouse anti-dynein (Santa Cruz, sc-13524, 1 :500), rabbit anti-KNSTRN (Sigma, HPA042027, 1:1000), rabbit anti-INSC (Abcam, ab102953, 1:1000), goat anti-NUMB (Abcam, ab4147, 1:100), mouse anti-dynactin (BD Biosciences, 610474, 1:500), rabbit anti-CLASP1 (Abcam, ab108620, 1:5000).

    Techniques: Expressing, Mouse Assay, Western Blot, Positive Control

    Complexes containing Caly and dynein are present in axons Endogenous DIC (A) and Lis1 (C) co-localized with endogenous Caly puncta in axons extended by adult rat sensory neurons in culture (arrows). GFP-DIC (B) and GFP-Lis1 (D) co-localized with mCh-Caly puncta in DRG axons. Scale Bars=5 μm. (E) Time-lapse data from two-minute recordings of 30 μm axon segments were used to generate the kymographs of particles moving in DRG axons. GFP-DIC and GFP-Lis1 were observed moving with mCh-Caly in the same particle. F. Dynein and Lis1 were pulled down by GST-Caly from brain extracts. Equal amounts of GST and GST-Caly-C were incubated with brain extracts, eluted proteins were probed with anti-DIC, anti-Lis1 and anti-GST antibodies. Only GST-Caly showed the capacity to bind dynein and Lis1.

    Journal: The international journal of biochemistry & cell biology

    Article Title: Dynein Binds and Stimulates Axonal Motility of the Endosome Adaptor and NEEP21 Family Member, Calcyon

    doi: 10.1016/j.biocel.2017.07.005

    Figure Lengend Snippet: Complexes containing Caly and dynein are present in axons Endogenous DIC (A) and Lis1 (C) co-localized with endogenous Caly puncta in axons extended by adult rat sensory neurons in culture (arrows). GFP-DIC (B) and GFP-Lis1 (D) co-localized with mCh-Caly puncta in DRG axons. Scale Bars=5 μm. (E) Time-lapse data from two-minute recordings of 30 μm axon segments were used to generate the kymographs of particles moving in DRG axons. GFP-DIC and GFP-Lis1 were observed moving with mCh-Caly in the same particle. F. Dynein and Lis1 were pulled down by GST-Caly from brain extracts. Equal amounts of GST and GST-Caly-C were incubated with brain extracts, eluted proteins were probed with anti-DIC, anti-Lis1 and anti-GST antibodies. Only GST-Caly showed the capacity to bind dynein and Lis1.

    Article Snippet: Alternatively, brain proteins pulled-down from brain were resolved on gradient gels were transferred to polyvinylidene fluoride (PVDF) membranes, and probed with anti-DIC (sc-13524, Santa Cruz), anti-Lis1 (in-house rabbit polyclonal antibody) or anti-GST (sc-459, Santa Cruz) antibodies.

    Techniques: Incubation

    Lis1 and Dynein overexpression increases the motility of mCh-Caly organelles in DRG axons (A) Kymographs of mCh-Caly organelles in axons overexpressing Lis1 and DIC. (B, C) Overexpression of Lis1 but not DIC increased the percentage of organelles classified as retrograde (numbers above bars is the number of axons analyzed for each condition). (D, E) Overexpression of Lis1 or DIC increased the speeds and run lengths of retrograde motile events. Lis1 increased the speed of anterograde motile events, and both Lis1 and DIC increased run lengths of anterograde motile events. (B) * p

    Journal: The international journal of biochemistry & cell biology

    Article Title: Dynein Binds and Stimulates Axonal Motility of the Endosome Adaptor and NEEP21 Family Member, Calcyon

    doi: 10.1016/j.biocel.2017.07.005

    Figure Lengend Snippet: Lis1 and Dynein overexpression increases the motility of mCh-Caly organelles in DRG axons (A) Kymographs of mCh-Caly organelles in axons overexpressing Lis1 and DIC. (B, C) Overexpression of Lis1 but not DIC increased the percentage of organelles classified as retrograde (numbers above bars is the number of axons analyzed for each condition). (D, E) Overexpression of Lis1 or DIC increased the speeds and run lengths of retrograde motile events. Lis1 increased the speed of anterograde motile events, and both Lis1 and DIC increased run lengths of anterograde motile events. (B) * p

    Article Snippet: Alternatively, brain proteins pulled-down from brain were resolved on gradient gels were transferred to polyvinylidene fluoride (PVDF) membranes, and probed with anti-DIC (sc-13524, Santa Cruz), anti-Lis1 (in-house rabbit polyclonal antibody) or anti-GST (sc-459, Santa Cruz) antibodies.

    Techniques: Over Expression