dynabeads oligo  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dynabeads oligo
    Dynabeads Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads oligo/product/Thermo Fisher
    Average 99 stars, based on 201 article reviews
    Price from $9.99 to $1999.99
    dynabeads oligo - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: In addition, the presence of proper ACT protein expression in astrocytes of the PDGF-hAPP/V717F (+/−)ACT(+/−) mice and its absence in the PDGF-hAPP/V717F (+/−)ACT(−/−) mice was verified by ACT immunohistochemistry in all of the pathologically examined animals. .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Sequencing:

    Article Title: Spt6 is required for the fidelity of promoter selection
    Article Snippet: Paragraph title: Transcription start site sequencing ... Poly(A)-enriched RNA was isolated from 300 ∝g of total RNA with 300 ∝l of Dynabeads oligo(dT)25 (Invitrogen), using the manufacturer’s instructions and eluted in water.

    Agarose Gel Electrophoresis:

    Article Title: Dynamics in the tomato root transcriptome on infection with the potato cyst nematode Globodera rostochiensis
    Article Snippet: The integrity of the total RNA was verified on a denaturing agarose gel, and the total RNA concentrations were measured spectrophotometrically. .. Dynabeads Oligo (dT)25 (Dynal A. S., Oslo, Norway) were used to isolate mRNA from each of the samples.

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: The DNA was linearized with Eco RV and Sca I, and the 10 kbp fragment was separated on a low melting point agarose gel, purified with β-agarase according to manufacturer's instructions (FMC Bioproducts, Rockland, ME), and injected into pronuclei of FVB/N zygotes at the Core Transgenic Mouse Facility (Brigham and Women's Hospital, Harvard Medical School, Boston, MA). .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Concentration Assay:

    Article Title: Spt6 is required for the fidelity of promoter selection
    Article Snippet: After determining the cell concentration using a hemocytometer, S. pombe cells (strain 972) were added to each culture at a level of 10%, to be used for spike-in normalization. .. Poly(A)-enriched RNA was isolated from 300 ∝g of total RNA with 300 ∝l of Dynabeads oligo(dT)25 (Invitrogen), using the manufacturer’s instructions and eluted in water.

    Isolation:

    Article Title: Spt6 is required for the fidelity of promoter selection
    Article Snippet: .. Poly(A)-enriched RNA was isolated from 300 ∝g of total RNA with 300 ∝l of Dynabeads oligo(dT)25 (Invitrogen), using the manufacturer’s instructions and eluted in water. .. Prior to each subsequent step of library construction, RNA samples were heat denatured at 80°C for two minutes and rapidly cooled on ice, followed by addition of 40 U of RNasin (Promega).

    Article Title: Dynamics in the tomato root transcriptome on infection with the potato cyst nematode Globodera rostochiensis
    Article Snippet: Paragraph title: Tissue sample collection and RNA isolation ... Dynabeads Oligo (dT)25 (Dynal A. S., Oslo, Norway) were used to isolate mRNA from each of the samples.

    Infection:

    Article Title: Dynamics in the tomato root transcriptome on infection with the potato cyst nematode Globodera rostochiensis
    Article Snippet: At time points 1, 3, 7 and 14 dpi, tomato root segments (length, ≈1 cm) from both infected (as detected by microscopy) and uninfected roots were collected and placed in RNase‐free tubes (≈400 mg of fresh weight per sample). .. Dynabeads Oligo (dT)25 (Dynal A. S., Oslo, Norway) were used to isolate mRNA from each of the samples.

    Purification:

    Article Title: Spt6 is required for the fidelity of promoter selection
    Article Snippet: Poly(A)-enriched RNA was isolated from 300 ∝g of total RNA with 300 ∝l of Dynabeads oligo(dT)25 (Invitrogen), using the manufacturer’s instructions and eluted in water. .. Between each enzymatic reaction, samples were purified using an RNA binding column (Zymo Research).

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: The DNA was linearized with Eco RV and Sca I, and the 10 kbp fragment was separated on a low melting point agarose gel, purified with β-agarase according to manufacturer's instructions (FMC Bioproducts, Rockland, ME), and injected into pronuclei of FVB/N zygotes at the Core Transgenic Mouse Facility (Brigham and Women's Hospital, Harvard Medical School, Boston, MA). .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Electrophoresis:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY). .. The RNA samples were heat-denatured (70°C) in 50% formamide, 2.2 m formaldehyde, 1 m 4-morpholinepropanesulfonic acid buffer, pH 7.0, and separated by electrophoresis on a 1.0% agarose-formaldehyde gel, blotted onto Hybond-N+ filters, and baked for 2 hr at 80°C.

    Transgenic Assay:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: The DNA was linearized with Eco RV and Sca I, and the 10 kbp fragment was separated on a low melting point agarose gel, purified with β-agarase according to manufacturer's instructions (FMC Bioproducts, Rockland, ME), and injected into pronuclei of FVB/N zygotes at the Core Transgenic Mouse Facility (Brigham and Women's Hospital, Harvard Medical School, Boston, MA). .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Incubation:

    Article Title: Dynamics in the tomato root transcriptome on infection with the potato cyst nematode Globodera rostochiensis
    Article Snippet: For total RNA isolation, aliquots of about 100 mg of frozen tomato root segments were incubated in 0.75 mL TRIzol Reagent (Gibco/Life Technologies, Breda, The Netherlands). .. Dynabeads Oligo (dT)25 (Dynal A. S., Oslo, Norway) were used to isolate mRNA from each of the samples.

    Activated Clotting Time Assay:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: In addition, the presence of proper ACT protein expression in astrocytes of the PDGF-hAPP/V717F (+/−)ACT(+/−) mice and its absence in the PDGF-hAPP/V717F (+/−)ACT(−/−) mice was verified by ACT immunohistochemistry in all of the pathologically examined animals. .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Microscopy:

    Article Title: Dynamics in the tomato root transcriptome on infection with the potato cyst nematode Globodera rostochiensis
    Article Snippet: At time points 1, 3, 7 and 14 dpi, tomato root segments (length, ≈1 cm) from both infected (as detected by microscopy) and uninfected roots were collected and placed in RNase‐free tubes (≈400 mg of fresh weight per sample). .. Dynabeads Oligo (dT)25 (Dynal A. S., Oslo, Norway) were used to isolate mRNA from each of the samples.

    Expressing:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: In addition, the presence of proper ACT protein expression in astrocytes of the PDGF-hAPP/V717F (+/−)ACT(+/−) mice and its absence in the PDGF-hAPP/V717F (+/−)ACT(−/−) mice was verified by ACT immunohistochemistry in all of the pathologically examined animals. .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Polymerase Chain Reaction:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: Mice from one of these founder lines (#8784) were crossed with homozygous PDGF-hAPP/V717F mice , and all offspring were screened with PCR for the GFAP-ACT and the PDGF-hAPP gene constructs. .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Construct:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: Mice from one of these founder lines (#8784) were crossed with homozygous PDGF-hAPP/V717F mice , and all offspring were screened with PCR for the GFAP-ACT and the PDGF-hAPP gene constructs. .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Injection:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: The DNA was linearized with Eco RV and Sca I, and the 10 kbp fragment was separated on a low melting point agarose gel, purified with β-agarase according to manufacturer's instructions (FMC Bioproducts, Rockland, ME), and injected into pronuclei of FVB/N zygotes at the Core Transgenic Mouse Facility (Brigham and Women's Hospital, Harvard Medical School, Boston, MA). .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Mouse Assay:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: In addition, the presence of proper ACT protein expression in astrocytes of the PDGF-hAPP/V717F (+/−)ACT(+/−) mice and its absence in the PDGF-hAPP/V717F (+/−)ACT(−/−) mice was verified by ACT immunohistochemistry in all of the pathologically examined animals. .. Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY).

    Labeling:

    Article Title: α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease
    Article Snippet: Total RNA was extracted with RNA Stat-60-kit (Tel-Test), and poly(A+ ) mRNA was selected with Dynabeads Oligo(dt)25 (Dynal, Great Neck, NY). .. Filters were prehybridized overnight and hybridized with P32 -labeled hACT and hGAPDH-cRNA probes ( ) in 50% formamide, 120 m m Tris-HCl, 8 m m EDTA, 600 m m NaCl, 1% nonfat dry milk powder, and 1% SDS.

    RNA Binding Assay:

    Article Title: Spt6 is required for the fidelity of promoter selection
    Article Snippet: Poly(A)-enriched RNA was isolated from 300 ∝g of total RNA with 300 ∝l of Dynabeads oligo(dT)25 (Invitrogen), using the manufacturer’s instructions and eluted in water. .. Between each enzymatic reaction, samples were purified using an RNA binding column (Zymo Research).

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    Thermo Fisher dynabeads oligo dt 25
    Dynabeads Oligo Dt 25, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads oligo dt 25/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
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    Thermo Fisher dynabeads mrna direct purification kit
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    https://www.bioz.com/result/dynabeads mrna direct purification kit/product/Thermo Fisher
    Average 90 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    dynabeads mrna direct purification kit - by Bioz Stars, 2020-01
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