dynabeads oligo  (Thermo Fisher)


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    • 99
    Name:
    Oligo dT Primer 50 µM
    Description:
    These are the same Ambion primers that are currently included in the RETROscript Kit SKU AM1710 They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript Kit
    Catalog Number:
    am5730g
    Price:
    None
    Applications:
    PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR
    Category:
    Oligos Primers Probes Nucleotides
    		Buy from Supplier

    Structured Review

    Thermo Fisher dynabeads oligo
    These are the same Ambion primers that are currently included in the RETROscript Kit SKU AM1710 They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript Kit
    https://www.bioz.com/result/dynabeads oligo/product/Thermo Fisher
    Average 99 stars, based on 179 article reviews
    Price from $9.99 to $1999.99
    dynabeads oligo - by Bioz Stars, 2020-07
    99/100 stars

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    Images

    Related Articles

    Transfection:

    Article Title: A redox mechanism underlying nucleolar stress sensing by nucleophosmin
    Article Snippet: .. Plasmids DNA and siRNA oligo were transfected into cells with Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: Glucocorticoid impairs cell-cell communication by autophagy-mediated degradation of connexin 43 in osteocytes
    Article Snippet: .. Transfection MLO-Y4 cells (1×105 /well) were plated in type-I collagen coated six-well culture plates, and then transfected with various concentrations (12.5nM, 25nM, 50nM and 100nM) of Atg5 siRNA oligo cocktail (Stealth siRNAs: MSS247019, MSS247020, MSS247021, from Thermo Fisher® , USA) or negative control using Lipofectamine® 3000 Transfection Reagent (Invitrogen® , USA) following the manufacturer's protocol. .. Confocal microscopy MLO-Y4 cells cultured on glass coverslips and calvarial primary osteocytes ex vivo cultured in 96-wells plates were fixed for 15mins and and 2hrs at RT with 4% PFA respectively.

    Article Title: miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors
    Article Snippet: .. Cell Transfection Cells were transfected with 30 mM Ambion pre-miR-411 precursor oligos (Life Technologies) using Lipofectamine 2000 (Life Technologies) following the manufacture protocol. .. Briefly, 4×103 /cm2 C2 C12 cells were plated in growth medium one day before the transfection.

    Article Title: Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells
    Article Snippet: .. RNAi was performed by the transfection of siRNA oligos using the Lipofectamine™ RNAiMAX transfection reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions. ..

    Amplification:

    Article Title: iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture
    Article Snippet: .. We then amplified the oligos in each well by real-time PCR using the SYBR Select Master Mix (Thermo Fisher Scientific, cat. no. 4472913). .. We designed PCR primers that would anneal to the F and R adapters specific for each oligo in the pool, and incorporate the C adapter and T7 promoter sequence, on the 5’ side of the F and R adapters, respectively.

    Synthesized:

    Article Title: Human RAD9 checkpoint control/proapoptotic protein can activate transcription of p21
    Article Snippet: .. Complementary oligos containing the p53-binding site were synthesized commercially (Invitrogen) and annealed in buffer (100 mM NaCl/5 mM Tris·Cl, pH 7.5/10 mM MgCl2 /0.02 mM EDTA/1 mM DTT). .. These probes were end-labeled with [γ-32 P]ATP by using T4 polynucleotide kinase (New England Biolabs).

    Magnetic Beads:

    Article Title: Transposable elements become active and mobile in the genomes of aging mammalian somatic tissues
    Article Snippet: .. Polyadenylated mRNA was prepared using two consecutive purifications with oligo-dT magnetic beads, following the mRNA Direct Dynabeads Kit protocol provided by the manufacturer (Invitrogen). .. The eluted mRNA was quantified using the Qubit 2.0 RNA HS Assay Kit (Invitrogen).

    Negative Control:

    Article Title: Glucocorticoid impairs cell-cell communication by autophagy-mediated degradation of connexin 43 in osteocytes
    Article Snippet: .. Transfection MLO-Y4 cells (1×105 /well) were plated in type-I collagen coated six-well culture plates, and then transfected with various concentrations (12.5nM, 25nM, 50nM and 100nM) of Atg5 siRNA oligo cocktail (Stealth siRNAs: MSS247019, MSS247020, MSS247021, from Thermo Fisher® , USA) or negative control using Lipofectamine® 3000 Transfection Reagent (Invitrogen® , USA) following the manufacturer's protocol. .. Confocal microscopy MLO-Y4 cells cultured on glass coverslips and calvarial primary osteocytes ex vivo cultured in 96-wells plates were fixed for 15mins and and 2hrs at RT with 4% PFA respectively.

    Real-time Polymerase Chain Reaction:

    Article Title: iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture
    Article Snippet: .. We then amplified the oligos in each well by real-time PCR using the SYBR Select Master Mix (Thermo Fisher Scientific, cat. no. 4472913). .. We designed PCR primers that would anneal to the F and R adapters specific for each oligo in the pool, and incorporate the C adapter and T7 promoter sequence, on the 5’ side of the F and R adapters, respectively.

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    • rna  (Thermo Fisher)
    99
    Thermo Fisher rna
    Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified <t>mRNA</t> from total <t>RNA.</t> Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/Thermo Fisher
    Average 99 stars, based on 3653 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-07
    99/100 stars
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    oligo dt magnetic beads  (Thermo Fisher)
    93
    Thermo Fisher oligo dt magnetic beads
    Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified <t>mRNA</t> from total <t>RNA.</t> Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p
    Oligo Dt Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt magnetic beads/product/Thermo Fisher
    Average 93 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    oligo dt magnetic beads - by Bioz Stars, 2020-07
    93/100 stars
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    oligo  (Thermo Fisher)
    99
    Thermo Fisher oligo
    Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified <t>mRNA</t> from total <t>RNA.</t> Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p
    Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1662 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo/product/Thermo Fisher
    Average 99 stars, based on 1662 article reviews
    Price from $9.99 to $1999.99
    oligo - by Bioz Stars, 2020-07
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified mRNA from total RNA. Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p

    Journal: The Journal of Immunology Author Choice

    Article Title: Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response

    doi: 10.4049/jimmunol.1801151

    Figure Lengend Snippet: Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified mRNA from total RNA. Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p

    Article Snippet: mRNA was purified from total RNA using Dynabeads Oligo(dT)25 (Thermo Fisher Scientific).

    Techniques: Inhibition, Methylation, Gas Chromatography-Mass Spectrometry, Dot Blot, Purification, Staining

    Quantitative Proteomics of ATF4 Translation (A) Schematic of SILAC. MEF cells were cultured in “light” or “heavy” media for 5 passages before treating “heavy” cells with amino acid starvation for 2 hr. Atf4 mRNA and associated proteins were purified by a biotinylated probe followed by mass spectrometry. (B) Relative ratio of Atf4 mRNA-associated 60S and 40S ribosomal proteins before and after amino acid starvation. Two biological replicates are shown. (C) A scatter plot shows Atf4 mRNA-associated proteins before and after amino acid starvation. The original peptide score (log 2 ) and starvation-induced fold changes are shown in the x-axis and the y-axis, respectively. RNA-binding proteins (RBPs) are highlighted with ALKBH5 indicated. (D) Validation of Atf4 mRNA-associated ALKBH5 by immunoblotting using the same sample as (D). (E) Schematic of zero distance cross linking methodology. 4-Thiouridine (s 4 U)-labelled RNAs were crosslinked to directly associated proteins using 365 nm UV. Gapdh or Atf4 mRNAs were purified by biotinylated probes followed by immunoblotting. .

    Journal: Molecular cell

    Article Title: N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response

    doi: 10.1016/j.molcel.2018.01.019

    Figure Lengend Snippet: Quantitative Proteomics of ATF4 Translation (A) Schematic of SILAC. MEF cells were cultured in “light” or “heavy” media for 5 passages before treating “heavy” cells with amino acid starvation for 2 hr. Atf4 mRNA and associated proteins were purified by a biotinylated probe followed by mass spectrometry. (B) Relative ratio of Atf4 mRNA-associated 60S and 40S ribosomal proteins before and after amino acid starvation. Two biological replicates are shown. (C) A scatter plot shows Atf4 mRNA-associated proteins before and after amino acid starvation. The original peptide score (log 2 ) and starvation-induced fold changes are shown in the x-axis and the y-axis, respectively. RNA-binding proteins (RBPs) are highlighted with ALKBH5 indicated. (D) Validation of Atf4 mRNA-associated ALKBH5 by immunoblotting using the same sample as (D). (E) Schematic of zero distance cross linking methodology. 4-Thiouridine (s 4 U)-labelled RNAs were crosslinked to directly associated proteins using 365 nm UV. Gapdh or Atf4 mRNAs were purified by biotinylated probes followed by immunoblotting. .

    Article Snippet: mRNA was purified from total RNA using Dynabeads® Oligo(dT)25 (Thermo Fisher).

    Techniques: Cell Culture, Purification, Mass Spectrometry, RNA Binding Assay