dynabeads oligo dt 25  (Thermo Fisher)


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    Name:
    Dynabeads Oligo dT 25
    Description:
    Dynabeads Oligo dT 25 mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information bearing nucleic acid is mRNA Since mRNA comprises only about 1 5 ot total cellular RNA the isolation of total RNA is not the most efficient way to isolate mRNA Other technologies designed to purify total RNA yield 80 ribosomal RNA and force mRNA to compete with ribosomal RNA transfer RNA micro RNA small nucleolar RNA and small cytoplasmic RNA for membrane binding Advantages of Dynabeads Oligo dT 25 beads • Fast and gentle procedure yields pure intact mRNA• Extremely pure mRNA isolation best choice upstream of cDNA synthesis• Exquisitely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra small starting samples enables cDNA library construction from a single cell How the beads workThe oligo dT 25 coated Dynabeads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples Ribosomal RNA DNA proteins and small RNA molecules such as transfer RNA micro RNA and small nucleolar RNA do not bind to the beads and are discarded Only polyadenylated RNA species mRNA are captured Isolated mRNA is pure eliminating the need for ribosomal RNA subtraction or a post extraction DNase treatment This column free system ensures the highest transcriptome recovery • Physical mRNA capture on mobile magnetic beads• Rapid and gentle magnetic handling procedures• No mRNA lost during high g force spins• No mRNA trapped in column membranes during elutionApplicationsmRNA is suitable for all downstream molecular applications including gene cloning cDNA synthesis cDNA library construction RT PCR quantitative RT PCR RPA Ribonuclease Protection Assay subtractive hybridization primer extension SAGE RACE and others The Dynabeads Oligo dT 25 mRNA isolation beads are the ideal mRNA purification method prior to cDNA library construction Use of these beads ensures the highest recovery and enrichment of the transcriptome These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation Verastile elution optionsElution can be performed in any volume down to 5 µL mRNA elution is optional because enzymatic reactions in downstream procedures are not inhibited by presence of Dynabeads Additionally one can perform cDNA synthesis directly on the beads to create a reusable solid phase cDNA library
    Catalog Number:
    61002
    Price:
    None
    Applications:
    Automated mRNA Isolation|Bead-Based IVD Assay Development|Bead-Based Nucleic Acid IVD|Clinical|DNA & RNA Purification & Analysis|Diagnostic Development|Molecular Diagnostic Test Development|RNA Extraction|mRNA Isolation|Automated Nucleic Acid Purification
    Category:
    Beads Microspheres
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    Structured Review

    Thermo Fisher dynabeads oligo dt 25
    Dynabeads Oligo dT 25 mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information bearing nucleic acid is mRNA Since mRNA comprises only about 1 5 ot total cellular RNA the isolation of total RNA is not the most efficient way to isolate mRNA Other technologies designed to purify total RNA yield 80 ribosomal RNA and force mRNA to compete with ribosomal RNA transfer RNA micro RNA small nucleolar RNA and small cytoplasmic RNA for membrane binding Advantages of Dynabeads Oligo dT 25 beads • Fast and gentle procedure yields pure intact mRNA• Extremely pure mRNA isolation best choice upstream of cDNA synthesis• Exquisitely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra small starting samples enables cDNA library construction from a single cell How the beads workThe oligo dT 25 coated Dynabeads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples Ribosomal RNA DNA proteins and small RNA molecules such as transfer RNA micro RNA and small nucleolar RNA do not bind to the beads and are discarded Only polyadenylated RNA species mRNA are captured Isolated mRNA is pure eliminating the need for ribosomal RNA subtraction or a post extraction DNase treatment This column free system ensures the highest transcriptome recovery • Physical mRNA capture on mobile magnetic beads• Rapid and gentle magnetic handling procedures• No mRNA lost during high g force spins• No mRNA trapped in column membranes during elutionApplicationsmRNA is suitable for all downstream molecular applications including gene cloning cDNA synthesis cDNA library construction RT PCR quantitative RT PCR RPA Ribonuclease Protection Assay subtractive hybridization primer extension SAGE RACE and others The Dynabeads Oligo dT 25 mRNA isolation beads are the ideal mRNA purification method prior to cDNA library construction Use of these beads ensures the highest recovery and enrichment of the transcriptome These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation Verastile elution optionsElution can be performed in any volume down to 5 µL mRNA elution is optional because enzymatic reactions in downstream procedures are not inhibited by presence of Dynabeads Additionally one can perform cDNA synthesis directly on the beads to create a reusable solid phase cDNA library
    https://www.bioz.com/result/dynabeads oligo dt 25/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dynabeads oligo dt 25 - by Bioz Stars, 2020-08
    99/100 stars

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    RNA Extraction:

    Article Title: On the cellular and developmental lethality of a Xenopus nucleocytoplasmic hybrid
    Article Snippet: .. Total RNA was extracted from the oocytes using a standard Trizol (Invitrogen) based method, followed by poly(A)+ RNA extraction using the Dynabeads® Oligo (dT)25 system (Invitrogen), according to the manufacturer’s recommendations. .. Fertilized enucleated eggs were de-jellied using a 2% L-Cysteine (Sigma) (pH 8) solution, placed in a 6% Ficoll (type 400, Sigma), 0.4x MMR solution, and injected at the one-cell stage using a Drummond micro-injector.

    RNA Sequencing Assay:

    Article Title: Correction: Transcriptome Analysis of Plant Hormone-Related Tomato (Solanum lycopersicum) Genes in a Sunlight-Type Plant Factory
    Article Snippet: .. There are errors in the first paragraph of the Materials and Methods section under the heading “RNA-Seq library preparation and sequence analyses.” The correct paragraph is: To prepare RNA-Seq library, the extracted total RNA was mixed with ERCC RNA Spike-In control mixes (Life Technologies, Carlsbad, CA, USA) and then polyA RNA was isolated using oligo(dT)25 Dynabeads (Invitrogen) (see reference 23). .. Briefly, the purified polyA RNA was fragmented by heat treatment, and reverse transcription was performed using M-MuLV Reverse Transcriptase (Enzymatics) and random hexamers.

    Magnetic Beads:

    Article Title: A New Mouse Gene, SRG3, Related to the SWI3 of Saccharomyces cerevisiae, Is Required for Apoptosis Induced by Glucocorticoids in a Thymoma Cell Line
    Article Snippet: .. ∼10 μg of poly(A)+ RNA was heated at 65°C for 2 min and annealed with 1 mg of magnetic beads containing oligo (dT) (Dynabeads oligo [dT]25 ; Dynal, Inc., Great Neck, NY) for 30 min at room temperature ( ). ..

    Isolation:

    Article Title: A small nuclear RNA, hdm365, is the major processing product of the human mdm2 gene
    Article Snippet: .. Poly(A)+ RNA was isolated from total RNA by binding to oligo(dT)25 Dynabeads (Dynal; Hamburg, Germany) according to the manufacturer’s recommendations. ..

    Article Title: Correction: Transcriptome Analysis of Plant Hormone-Related Tomato (Solanum lycopersicum) Genes in a Sunlight-Type Plant Factory
    Article Snippet: .. There are errors in the first paragraph of the Materials and Methods section under the heading “RNA-Seq library preparation and sequence analyses.” The correct paragraph is: To prepare RNA-Seq library, the extracted total RNA was mixed with ERCC RNA Spike-In control mixes (Life Technologies, Carlsbad, CA, USA) and then polyA RNA was isolated using oligo(dT)25 Dynabeads (Invitrogen) (see reference 23). .. Briefly, the purified polyA RNA was fragmented by heat treatment, and reverse transcription was performed using M-MuLV Reverse Transcriptase (Enzymatics) and random hexamers.

    Article Title: A role for small secreted proteins (SSPs) in a saprophytic fungal lifestyle: Ligninolytic enzyme regulation in Pleurotus ostreatus
    Article Snippet: .. cDNA libraries preparation and RNAseq The mRNA from biological triplicates was isolated from 10 µg total RNA samples using Dynabeads Oligo(dT)25 (Invitrogen) and fragmented by the RNA Fragmentation Reagents kit (Ambion). ..

    Article Title: Abundant Defective Viral Particles Budding from Microglia in the Course of Retroviral Spongiform Encephalopathy
    Article Snippet: .. mRNA was isolated from cells and tissues, employing oligo(dT)25 Dynabeads following the recommendations of the manufacturer (Dynal). .. After Northern blotting, blots were probed with a viral envelope-specific 32 P-labeled probe ( ) as well as with a 32 P-labeled probe for a housekeeping gene, rat glyceraldehyde-3-phosphate dehydrogenase.

    Sequencing:

    Article Title: Correction: Transcriptome Analysis of Plant Hormone-Related Tomato (Solanum lycopersicum) Genes in a Sunlight-Type Plant Factory
    Article Snippet: .. There are errors in the first paragraph of the Materials and Methods section under the heading “RNA-Seq library preparation and sequence analyses.” The correct paragraph is: To prepare RNA-Seq library, the extracted total RNA was mixed with ERCC RNA Spike-In control mixes (Life Technologies, Carlsbad, CA, USA) and then polyA RNA was isolated using oligo(dT)25 Dynabeads (Invitrogen) (see reference 23). .. Briefly, the purified polyA RNA was fragmented by heat treatment, and reverse transcription was performed using M-MuLV Reverse Transcriptase (Enzymatics) and random hexamers.

    RNA Binding Assay:

    Article Title: A small nuclear RNA, hdm365, is the major processing product of the human mdm2 gene
    Article Snippet: .. Poly(A)+ RNA was isolated from total RNA by binding to oligo(dT)25 Dynabeads (Dynal; Hamburg, Germany) according to the manufacturer’s recommendations. ..

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    Thermo Fisher rna
    Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified <t>mRNA</t> from total <t>RNA.</t> Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/Thermo Fisher
    Average 99 stars, based on 3832 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher oligo
    Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified <t>mRNA</t> from total <t>RNA.</t> Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p
    Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1662 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo/product/Thermo Fisher
    Average 99 stars, based on 1662 article reviews
    Price from $9.99 to $1999.99
    oligo - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified mRNA from total RNA. Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p

    Journal: The Journal of Immunology Author Choice

    Article Title: Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response

    doi: 10.4049/jimmunol.1801151

    Figure Lengend Snippet: Inhibition effect of LPS on LCFA absorption in IPEC-J2 cells was related to m 6 A methylation. IPEC-J2 cells were treated with 10 μg/ml LPS for various times, and cells without LPS stimulation were used as controls. ( A ) The composition of fatty acids in whole lysate measured by GC/MS. ( B ) m 6 A dot blot to measure the m 6 A levels with purified mRNA from total RNA. Methylene blue staining was used for loading control. The right panel shows the relative levels quantified by densitometry and normalized to control. The data are expressed as the mean ± SEM. Statistically significant difference relative to the control (0 h) ( n = 3, biological replicates). * p

    Article Snippet: mRNA was purified from total RNA using Dynabeads Oligo(dT)25 (Thermo Fisher Scientific).

    Techniques: Inhibition, Methylation, Gas Chromatography-Mass Spectrometry, Dot Blot, Purification, Staining

    Quantitative Proteomics of ATF4 Translation (A) Schematic of SILAC. MEF cells were cultured in “light” or “heavy” media for 5 passages before treating “heavy” cells with amino acid starvation for 2 hr. Atf4 mRNA and associated proteins were purified by a biotinylated probe followed by mass spectrometry. (B) Relative ratio of Atf4 mRNA-associated 60S and 40S ribosomal proteins before and after amino acid starvation. Two biological replicates are shown. (C) A scatter plot shows Atf4 mRNA-associated proteins before and after amino acid starvation. The original peptide score (log 2 ) and starvation-induced fold changes are shown in the x-axis and the y-axis, respectively. RNA-binding proteins (RBPs) are highlighted with ALKBH5 indicated. (D) Validation of Atf4 mRNA-associated ALKBH5 by immunoblotting using the same sample as (D). (E) Schematic of zero distance cross linking methodology. 4-Thiouridine (s 4 U)-labelled RNAs were crosslinked to directly associated proteins using 365 nm UV. Gapdh or Atf4 mRNAs were purified by biotinylated probes followed by immunoblotting. .

    Journal: Molecular cell

    Article Title: N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response

    doi: 10.1016/j.molcel.2018.01.019

    Figure Lengend Snippet: Quantitative Proteomics of ATF4 Translation (A) Schematic of SILAC. MEF cells were cultured in “light” or “heavy” media for 5 passages before treating “heavy” cells with amino acid starvation for 2 hr. Atf4 mRNA and associated proteins were purified by a biotinylated probe followed by mass spectrometry. (B) Relative ratio of Atf4 mRNA-associated 60S and 40S ribosomal proteins before and after amino acid starvation. Two biological replicates are shown. (C) A scatter plot shows Atf4 mRNA-associated proteins before and after amino acid starvation. The original peptide score (log 2 ) and starvation-induced fold changes are shown in the x-axis and the y-axis, respectively. RNA-binding proteins (RBPs) are highlighted with ALKBH5 indicated. (D) Validation of Atf4 mRNA-associated ALKBH5 by immunoblotting using the same sample as (D). (E) Schematic of zero distance cross linking methodology. 4-Thiouridine (s 4 U)-labelled RNAs were crosslinked to directly associated proteins using 365 nm UV. Gapdh or Atf4 mRNAs were purified by biotinylated probes followed by immunoblotting. .

    Article Snippet: mRNA was purified from total RNA using Dynabeads® Oligo(dT)25 (Thermo Fisher).

    Techniques: Cell Culture, Purification, Mass Spectrometry, RNA Binding Assay