Journal: RSC Advances

Article Title: Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices †

doi: 10.1039/d4ra08290g

Figure Lengend Snippet: Cellular growth at different days (1, 3, 6) of organoid-derived intestinal epithelial cells on different substrates (APTMS, PEIGA, PDMS, plastic). The cells were cultured on different adhesion proteins: Laminin 111 (A), Laminin 511 (B), Collagen I and Matrigel (C), Collagen I and Laminin 511 (D) and in ENR medium. The data points represent the means and error bars the standard deviations. N = 3. (E) Growth dynamics showing representative images of organoid-derived intestinal epithelial cells, cultured in the microchannels as outline by the white dashed lines, of the alternative covering the largest area from (A–D) respectively (nuclei blue, actin green). Scale bar = 200 μm.

Article Snippet: Cryopreserved mouse duodenum epithelial organoids (StemCell Technologies #70931) were thawed and entrapped in Matrigel (Corning) in an appropriate volume (25 μL for one dome).

Techniques: Derivative Assay, Cell Culture

Journal: RSC Advances

Article Title: Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices †

doi: 10.1039/d4ra08290g

Figure Lengend Snippet: Cellular growth at different days (1, 3, 6) of organoid-derived intestinal epithelial cells on different substrates (APTMS, PEIGA, PDMS, plastic). The cells were cultured on different adhesion proteins: Laminin 111 (A), Laminin 511 (B), Collagen I and Matrigel (C), Collagen I and Laminin 511 (D) in CV medium. The data points represent the means and error bars the standard deviations. N = 3. (E) Growth dynamics showing representative images of organoid-derived intestinal epithelial cells, cultured in the microchannels as outline by the white dashed lines, of the alternative covering the largest area from A–D respectively (nuclei blue, actin green). Scale bar = 200 μm.

Article Snippet: Cryopreserved mouse duodenum epithelial organoids (StemCell Technologies #70931) were thawed and entrapped in Matrigel (Corning) in an appropriate volume (25 μL for one dome).

Techniques: Derivative Assay, Cell Culture

Journal: RSC Advances

Article Title: Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices †

doi: 10.1039/d4ra08290g

Figure Lengend Snippet: Representative images of murine small intestinal epithelium at day 6. (A) and (B) Show the epithelial coverage with actin (green) and nuclei (blue) on PEIGA-functionalized PDMS and coated with Laminin 511 in either (A) CV, or (B) ENR media. (C) and (D) Display the same epithelium on plastic coated with Laminin 511 in the presence of either (C) CV medium, or (D) ENR medium. (E–L) Display actin in green, nuclei in blue, Ki67 in red and the colocalization pixels between nuclei and Ki67 in gray. (E) and (F) Display the epithelium on PEIGA-functionalized PDMS and Laminin 511 in presence of CV, (G) and (H) display the epithelium in ENR medium. (I) and (J) Display the epithelium in plastic and Laminin 511 in presence of CV. (K) and (L) Display the epithelium in ENR medium. Scale bar = 100 μm.

Article Snippet: Cryopreserved mouse duodenum epithelial organoids (StemCell Technologies #70931) were thawed and entrapped in Matrigel (Corning) in an appropriate volume (25 μL for one dome).

Techniques:

Journal: RSC Advances

Article Title: Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices †

doi: 10.1039/d4ra08290g

Figure Lengend Snippet: Representative images of human intestinal epithelium at day 6. (A) and (B) Show the epithelial coverage with actin (green) and nuclei (blue) in (A) plastic and (B) PDMS functionalized with PEIGA both coated with Laminin 511. The microfluidic channel is outlined by the white dashed lines. Scale bar = 200 μm.

Article Snippet: Cryopreserved mouse duodenum epithelial organoids (StemCell Technologies #70931) were thawed and entrapped in Matrigel (Corning) in an appropriate volume (25 μL for one dome).

Techniques: