dulbecco s phosphate buffered saline  (Millipore)

 
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  • 86
    Name:
    Dulbecco s Phosphate Buffered Saline
    Description:

    Catalog Number:
    d8537
    Price:
    None
    Applications:
    DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
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    Structured Review

    Millipore dulbecco s phosphate buffered saline

    https://www.bioz.com/result/dulbecco s phosphate buffered saline/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s phosphate buffered saline - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Purification:

    Article Title: Gelatin Type A from Porcine Skin Used as Co-Initiator in a Radical Photo-Initiating System
    Article Snippet: .. Materials Gelatin (type A, Vetec reagent grade powder, gel strength = 300 g Bloom, Mn = 50,000–100,000) from porcine skin, poly(ethylene glycol) diacrylate (PEGDA, Mn = 700 g mol−1 ), camphorquinone (CQ, 97%) and Dulbecco′s phosphate buffered saline (DPBS, pH 7.0–7.3) were purchased from Sigma-Aldrich (Milano, Italy) and used as received without further purification. .. Deionized water (DIH2 O) was obtained from a reverse osmosis (RO) purification system.

    other:

    Article Title: Long-Term Modeling of SARS-CoV-2 Infection of In Vitro Cultured Polarized Human Airway Epithelium
    Article Snippet: Transepithelial electrical resistance (TEER).One hundred microliters of D-PBS was added to the apical chamber to determine the TEER using a Millicell ERS-2 volt-ohm meter (MilliporeSigma, Burlington, MA) following a previously used method ( ).

    Incubation:

    Article Title: Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes
    Article Snippet: After washing thrice with D-PBS, the oocytes were incubated with goat anti-MAD2 antibody (1:50 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) as a primary antibody over night at 4°C and then washed three times with D-PBS. .. This was followed by incubation in Alexa Fluor® 594-conjugated anti-goat IgG secondary antibody (1:500 dilution, Invitrogen) for 60 min. After washing thrice with D-PBS, the oocytes were incubated with mouse anti-α-Tubulin monoclonal antibody (1:500 dilution, Sigma) as a primary antibody for 60 min at room temperature, washed thrice with D-PBS, then incubated in fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG (1:100 dilution, Sigma) as a secondary antibody for 1 h at room temperature. .. Oocyte chromosomes were stained with 0.1 μg/ml 4',6-diamidino-2-phenylindole (DAPI, Sigma) for 30 min at 4°C.

    Article Title: Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci
    Article Snippet: VREfm Adherence to Caco-2 Cells In Vitro The adherence of VREfm to Caco-2 cells was inhibited using probiotics according to a previous procedure, with modifications [ ]. .. For the exclusion assay, Caco-2 cells in each well of 12-well plates forming confluent monolayers were washed twice with Dulbecco’s phosphate buffer saline (DPBS) (D5652, Sigma-Aldrich, St. Louis, MO, USA) and cultured with probiotic strains (approximately 3.33 × 108 CFU/mL) for 1 h. After incubation with probiotics, the Caco-2 cells were washed with DPBS twice and incubated with VREfm (approximately 3.33 × 106 CFU/mL) for another 1 h. Subsequently, the Caco-2 cells were washed twice with DPBS and then incubated with 0.05% Triton-X100 (T8787, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 20 min. ..

    Article Title: Norwalk Virus-Like Particle Hemagglutination by Binding to H Histo-Blood Group Antigens
    Article Snippet: .. Neuraminidase from Arthrobacter ureafaciens (6 μU; Sigma) in Dulbecco's PBS (D-PBS) (Invitrogen) was combined with the RBCs and incubated for 60 min at 37°C. l -(Toslylamido-2-phenyl)ethyl chloromethyl ketone-treated trypsin (1.25 ng; Sigma) in D-PBS was added to the RBCs and incubated for 30 min at 37°C. .. Trypsin activity was stopped by adding 0.5 μg of soybean trypsin inhibitor (Sigma) to the reaction mixture.

    Exclusion Assay:

    Article Title: Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci
    Article Snippet: VREfm Adherence to Caco-2 Cells In Vitro The adherence of VREfm to Caco-2 cells was inhibited using probiotics according to a previous procedure, with modifications [ ]. .. For the exclusion assay, Caco-2 cells in each well of 12-well plates forming confluent monolayers were washed twice with Dulbecco’s phosphate buffer saline (DPBS) (D5652, Sigma-Aldrich, St. Louis, MO, USA) and cultured with probiotic strains (approximately 3.33 × 108 CFU/mL) for 1 h. After incubation with probiotics, the Caco-2 cells were washed with DPBS twice and incubated with VREfm (approximately 3.33 × 106 CFU/mL) for another 1 h. Subsequently, the Caco-2 cells were washed twice with DPBS and then incubated with 0.05% Triton-X100 (T8787, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 20 min. ..

    Cell Culture:

    Article Title: Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci
    Article Snippet: VREfm Adherence to Caco-2 Cells In Vitro The adherence of VREfm to Caco-2 cells was inhibited using probiotics according to a previous procedure, with modifications [ ]. .. For the exclusion assay, Caco-2 cells in each well of 12-well plates forming confluent monolayers were washed twice with Dulbecco’s phosphate buffer saline (DPBS) (D5652, Sigma-Aldrich, St. Louis, MO, USA) and cultured with probiotic strains (approximately 3.33 × 108 CFU/mL) for 1 h. After incubation with probiotics, the Caco-2 cells were washed with DPBS twice and incubated with VREfm (approximately 3.33 × 106 CFU/mL) for another 1 h. Subsequently, the Caco-2 cells were washed twice with DPBS and then incubated with 0.05% Triton-X100 (T8787, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 20 min. ..

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  • 98
    Millipore elispot wash buffer
    IFN- γ expression by BAL cells and PBMCs following interaction with SHIV antigen-pulsed dendritic cells. Cells were seeded into 96-well plates and IFN- γ expression measured by <t>ELISPOT</t> assay. Results are expressed as the fold change from
    Elispot Wash Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elispot wash buffer/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elispot wash buffer - by Bioz Stars, 2021-03
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    97
    Millipore d pbs
    Virus release kinetics and <t>transepithelial</t> electrical resistance (TEER) measurement of HAE-ALI infected with SARS-CoV-2 at various viral loads (multiplicities of infection [MOIs]). (A and C) Virus release kinetics. HAE-ALI B4-20 cultures were infected with SARS-CoV-2 at an MOI of 0.2 (A) and 0.02 and 0.002 (C), respectively, from the apical side. At the indicated days postinfection (dpi), 100 μl of apical washes by incubation of 100 μl of <t>D-PBS</t> in the apical chamber and 100 μl of the basolateral media were taken for plaque assays. Plaque-forming units (PFU) were plotted to the dpi. Values represent means ± standard deviations. (B and D) TEER measurement. The TEER of mock- and SARS-CoV-2-infected HAE-ALI culture was measured using an epithelial volt-ohm meter (Millipore) at the indicated dpi. The TEER values were normalized to the TEER measured on the day of infection, which is set at 1.0. Values represent the means of relative TEER ± standard deviations. *** * , P
    D Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d pbs/product/Millipore
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    Image Search Results


    IFN- γ expression by BAL cells and PBMCs following interaction with SHIV antigen-pulsed dendritic cells. Cells were seeded into 96-well plates and IFN- γ expression measured by ELISPOT assay. Results are expressed as the fold change from

    Journal:

    Article Title: Comparison of the Effects of Pathogenic Simian Human Immunodeficiency Virus Strains SHIV-89.6P and SHIV-KU2 in Cynomolgus Macaques

    doi: 10.1089/aid.2007.0238

    Figure Lengend Snippet: IFN- γ expression by BAL cells and PBMCs following interaction with SHIV antigen-pulsed dendritic cells. Cells were seeded into 96-well plates and IFN- γ expression measured by ELISPOT assay. Results are expressed as the fold change from

    Article Snippet: Plates were incubated with antigen for 40 h at 37°C/5% CO2 , and then washed for 10 min with distilled water followed by four washes with ELISPOT wash buffer [D-PBS containing 0.05% Tween-20 (Sigma)].

    Techniques: Expressing, Enzyme-linked Immunospot

    Virus release kinetics and transepithelial electrical resistance (TEER) measurement of HAE-ALI infected with SARS-CoV-2 at various viral loads (multiplicities of infection [MOIs]). (A and C) Virus release kinetics. HAE-ALI B4-20 cultures were infected with SARS-CoV-2 at an MOI of 0.2 (A) and 0.02 and 0.002 (C), respectively, from the apical side. At the indicated days postinfection (dpi), 100 μl of apical washes by incubation of 100 μl of D-PBS in the apical chamber and 100 μl of the basolateral media were taken for plaque assays. Plaque-forming units (PFU) were plotted to the dpi. Values represent means ± standard deviations. (B and D) TEER measurement. The TEER of mock- and SARS-CoV-2-infected HAE-ALI culture was measured using an epithelial volt-ohm meter (Millipore) at the indicated dpi. The TEER values were normalized to the TEER measured on the day of infection, which is set at 1.0. Values represent the means of relative TEER ± standard deviations. *** * , P

    Journal: mBio

    Article Title: Long-Term Modeling of SARS-CoV-2 Infection of In Vitro Cultured Polarized Human Airway Epithelium

    doi: 10.1128/mBio.02852-20

    Figure Lengend Snippet: Virus release kinetics and transepithelial electrical resistance (TEER) measurement of HAE-ALI infected with SARS-CoV-2 at various viral loads (multiplicities of infection [MOIs]). (A and C) Virus release kinetics. HAE-ALI B4-20 cultures were infected with SARS-CoV-2 at an MOI of 0.2 (A) and 0.02 and 0.002 (C), respectively, from the apical side. At the indicated days postinfection (dpi), 100 μl of apical washes by incubation of 100 μl of D-PBS in the apical chamber and 100 μl of the basolateral media were taken for plaque assays. Plaque-forming units (PFU) were plotted to the dpi. Values represent means ± standard deviations. (B and D) TEER measurement. The TEER of mock- and SARS-CoV-2-infected HAE-ALI culture was measured using an epithelial volt-ohm meter (Millipore) at the indicated dpi. The TEER values were normalized to the TEER measured on the day of infection, which is set at 1.0. Values represent the means of relative TEER ± standard deviations. *** * , P

    Article Snippet: Transepithelial electrical resistance (TEER).One hundred microliters of D-PBS was added to the apical chamber to determine the TEER using a Millicell ERS-2 volt-ohm meter (MilliporeSigma, Burlington, MA) following a previously used method ( ).

    Techniques: Infection, Incubation

    Flow cytometric analysis of intracellular thiol redox status in primary human T-cells . PBMC’s were prepared from venous blood using Ficoll-Paque density centrifugation and washed in d -PBS. Washed PBMC’s (10 6 cells in 100 μl) were labelled with anti-human CD3-APC. PBMC’s were then treated with either 1 μM NEM followed by 0.1 μM F5M, or 0.1 μM F5M alone and analysed by flow cytometry. (A) Representative forward light scatter (FSC) vs side light scatter (SSC) flow cytometric profile with the treated PBMC’s identified in P2. (B) Gated on P2, CD3 + T-cells are shown in the FL4 parameter (laser: λ640 nm, filter: λ675/25 nm) in R1 (C). Panel shows representative fluorescence histograms depicting F5M fluorescence in control and treated T-cells (gated on R1). (D) T-cell viability was monitored before and after NEM and F5M treatment using trypan blue staining. Confocal microscopy was used to confirm (E) F5M cellular uptake in PBMCs, (F) surface staining for T-cells with anti-human CD3-APC and (G) F5M and T-cell overlay.

    Journal: MethodsX

    Article Title: Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers

    doi: 10.1016/j.mex.2018.10.013

    Figure Lengend Snippet: Flow cytometric analysis of intracellular thiol redox status in primary human T-cells . PBMC’s were prepared from venous blood using Ficoll-Paque density centrifugation and washed in d -PBS. Washed PBMC’s (10 6 cells in 100 μl) were labelled with anti-human CD3-APC. PBMC’s were then treated with either 1 μM NEM followed by 0.1 μM F5M, or 0.1 μM F5M alone and analysed by flow cytometry. (A) Representative forward light scatter (FSC) vs side light scatter (SSC) flow cytometric profile with the treated PBMC’s identified in P2. (B) Gated on P2, CD3 + T-cells are shown in the FL4 parameter (laser: λ640 nm, filter: λ675/25 nm) in R1 (C). Panel shows representative fluorescence histograms depicting F5M fluorescence in control and treated T-cells (gated on R1). (D) T-cell viability was monitored before and after NEM and F5M treatment using trypan blue staining. Confocal microscopy was used to confirm (E) F5M cellular uptake in PBMCs, (F) surface staining for T-cells with anti-human CD3-APC and (G) F5M and T-cell overlay.

    Article Snippet: 8 N- ethylmaleimide (NEM): 1 mM stock in d -PBS stored at 4 °C protected from light (Sigma-Aldrich, Dorset, UK; catalogue #E3876).

    Techniques: Flow Cytometry, Centrifugation, Cytometry, Fluorescence, Staining, Confocal Microscopy

    ( a ) Amplitude sweep curves and ( b ) swelling degree of the different hydrogel samples in Dulbecco′s phosphate buffered saline (DPBS) at 37 °C.

    Journal: Polymers

    Article Title: Gelatin Type A from Porcine Skin Used as Co-Initiator in a Radical Photo-Initiating System

    doi: 10.3390/polym11111901

    Figure Lengend Snippet: ( a ) Amplitude sweep curves and ( b ) swelling degree of the different hydrogel samples in Dulbecco′s phosphate buffered saline (DPBS) at 37 °C.

    Article Snippet: Materials Gelatin (type A, Vetec reagent grade powder, gel strength = 300 g Bloom, Mn = 50,000–100,000) from porcine skin, poly(ethylene glycol) diacrylate (PEGDA, Mn = 700 g mol−1 ), camphorquinone (CQ, 97%) and Dulbecco′s phosphate buffered saline (DPBS, pH 7.0–7.3) were purchased from Sigma-Aldrich (Milano, Italy) and used as received without further purification.

    Techniques: