dulbecco s phosphate buffered saline dpbs  (Thermo Fisher)


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    Name:
    Phosphate Buffered Saline
    Description:
    Recover microorganisms from multiple sample types including rinse samples from clinical instruments and environmental monitoring samples by creating a pH balanced environment with Thermo Scientific Phosphate Buffered Saline with Poly 80 PBST a buffer solution commonly used in biological research
    Catalog Number:
    r112502
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Blood Culture|Clinical|Clinical Microbiology
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    Structured Review

    Thermo Fisher dulbecco s phosphate buffered saline dpbs
    Recover microorganisms from multiple sample types including rinse samples from clinical instruments and environmental monitoring samples by creating a pH balanced environment with Thermo Scientific Phosphate Buffered Saline with Poly 80 PBST a buffer solution commonly used in biological research
    https://www.bioz.com/result/dulbecco s phosphate buffered saline dpbs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s phosphate buffered saline dpbs - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Murine cytomegalovirus degrades MHC class II to colonize the salivary glands
    Article Snippet: 6μM sections were blocked with 0.3% Triton X-100 / 5% normal goat serum, then incubated (18h, 4°C) with mAbs to MHC II (rat, M5/114) and MCMV IE1 (mouse IgG1 , CROMA101) [ ]. .. Sections were washed x3 in PBS, incubated (1h, 23°C) with Alexa 488-goat anti-mouse IgG1 and Alexa 568-goat anti-rat IgG pAb plus DAPI (1μg/ml), then washed x3 in PBS, and mounted in ProLong Gold (Life Technologies). .. Cultured cells were adhered to coverslips, then fixed (2% formaldehyde, 30min), blocked in PBS / 0.1% Triton-X100 / 1% bovine serum albumin, then stained with antibodies to MHC II (M5/114 or IBL5/22), CD44 (rat mAb IM7), MCMV IE1 (CROMA101), βgal (chicken pAb, AbCam), MCMV (rabbit pAb), and M78 (rabbit pAb) [ ].

    Article Title: TNFα/IFNγ Mediated Intestinal Epithelial Barrier Dysfunction Is Attenuated by MicroRNA-93 Downregulation of PTK6 in Mouse Colonic Epithelial Cells
    Article Snippet: .. Cells were incubated with primary antibodies (ZO-1, Cell Signaling/ FoxO1, Millipore) 16 hours at 4°C diluted 1:50 [PBS, 0.01% Tween-20, 3% BSA], washed 3 times for 10 minutes each [PBS, 0.1% Tween-20], incubated with appropriate secondary antibody diluted 1:200 [PBS, 0.01% Tween-20, 2% BSA] (AlexaFluor-conjugated; Life Technologies). .. Slides were mounted with anti-fade media containing DAPI (Life Technologies).

    Article Title: High Fat Diet Causes Depletion of Intestinal Eosinophils Associated with Intestinal Permeability
    Article Snippet: .. 2 x 106 cells were then incubated with 1:1000 dilution of Live dead aqua (Invitrogen, NY) and 1:100 dilution of anti CD16/32 antibody (eBioscience, San Diego, CA, 93) for 30 minutes on ice in PBS, washed twice and incubated with the following antibody panel in PBS containing 2%FCS, 2mM EDTA: CD45 NC605 (30-F11), F4/80PECy7 (BM8) (from eBioscience, San Diego, CA), CD11b-FITC (M170), Siglec F-PE (E50-2440), CD11c-APC or CD11c-v450 (HL3) (from BD Biosciences, San Jose, CA) and MHCII APCCy7 (Biolegend, San Diego, CA, M5/114.15.2). .. Cells were fixed overnight in BD stabilizing fixative (BD Biosciences, San Jose, CA), washed twice and acquired on a BD Canto RUO flow cytometer.

    Mouse Assay:

    Article Title: Reliability and reproducibility of a rodent model of choroidal neovascularization based on the subretinal injection of polyethylene glycol
    Article Snippet: To evaluate the reliability and reproducibility of a rodent choroidal neovascularization (CNV) model by subretinal injection of polyethylene glycol (PEG). .. C57BL/6 mice were injected subretinally with 2 μl PBS (Gibco, Invitrogen, Paisley, UK; n=14) or PEG (1 mg; n=18). .. Eyes were embedded in paraffin wax and serial sections were stained with haematoxylin and eosin or Fontana-Masson or immunostained for cytokeratin 8/18, isolectin B4 (IB4), vascular endothelial growth factor (VEGF) and von Willebrand factor (vWF).

    Injection:

    Article Title: Reliability and reproducibility of a rodent model of choroidal neovascularization based on the subretinal injection of polyethylene glycol
    Article Snippet: To evaluate the reliability and reproducibility of a rodent choroidal neovascularization (CNV) model by subretinal injection of polyethylene glycol (PEG). .. C57BL/6 mice were injected subretinally with 2 μl PBS (Gibco, Invitrogen, Paisley, UK; n=14) or PEG (1 mg; n=18). .. Eyes were embedded in paraffin wax and serial sections were stained with haematoxylin and eosin or Fontana-Masson or immunostained for cytokeratin 8/18, isolectin B4 (IB4), vascular endothelial growth factor (VEGF) and von Willebrand factor (vWF).

    Modification:

    Article Title: Oral delivery of siRNA lipid nanoparticles: Fate in the GI tract
    Article Snippet: Porcine pepsin, pancreatin, bile salts, and mucin type II were obtained from Sigma (St Louis, MO). .. Dulbecco’s Modified Eagles Media (DMEM), trypsin, penicillin/streptomyocin, phosphate buffered saline (PBS), fetal bovine serum (FBS), and GAPDH siRNA were purchased from Thermo Fisher (Waltham, MA). .. Corning BioCoat HTS 1 µm porous support Transwell plates, basal seeding medium (BSM), Corning EnteroSTIM enterocyte differentiation medium (EDM), and MITO + serum extender were obtained from VWR (Radnor, PA).

    Article Title: Indocyanine Green-Conjugated Magnetic Prussian Blue Nanoparticles for Synchronous Photothermal/Photodynamic Tumor Therapy
    Article Snippet: Indocyanine green (ICG), thiazolyl blue tetrazolium bromide (MTT, 98%), and dimethyl sulfoxide (DMSO) were supplied by Aladdin (China). .. Dulbecco’s modified Eagle medium (DMEM), TrypLE™ Express Enzyme, 4′,6-diamidino-2-phenylindole (DAPI), fetal bovine serum (FBS), penicillin/streptomycin solution, calcein-AM, and phosphate-buffered saline (PBS) were acquired from Thermo Fisher Scientific (USA). .. The One-Step TUNEL Apoptosis Assay Kit and the mitochondrial membrane potential assay kit with JC-1, and proteinase K were purchased from Beyotime Biotechnology (China).

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    Thermo Fisher slex ap
    (A) Schematic workflow of <t>CTC-</t> BioT Chip fabrication. HepG2 capture yields of <t>sLeX-AP</t> coated CTC- BioT Chip (B) at different sLeX-AP concentration (with concentration of 10, 20, 30, 40, 50, 60 μM) and (C) at different incubation time (15, 30, 45, 60, 90min). The error bar represents standard deviation from three repeats.
    Slex Ap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slex ap/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    slex ap - by Bioz Stars, 2021-03
    97/100 stars
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    (A) Schematic workflow of CTC- BioT Chip fabrication. HepG2 capture yields of sLeX-AP coated CTC- BioT Chip (B) at different sLeX-AP concentration (with concentration of 10, 20, 30, 40, 50, 60 μM) and (C) at different incubation time (15, 30, 45, 60, 90min). The error bar represents standard deviation from three repeats.

    Journal: Theranostics

    Article Title: Aptamer-Mediated Transparent-Biocompatible Nanostructured Surfaces for Hepotocellular Circulating Tumor Cells Enrichment

    doi: 10.7150/thno.15284

    Figure Lengend Snippet: (A) Schematic workflow of CTC- BioT Chip fabrication. HepG2 capture yields of sLeX-AP coated CTC- BioT Chip (B) at different sLeX-AP concentration (with concentration of 10, 20, 30, 40, 50, 60 μM) and (C) at different incubation time (15, 30, 45, 60, 90min). The error bar represents standard deviation from three repeats.

    Article Snippet: Cell capture and identification Prior to cell capture, CTC-BioT Chip was placed into a size-matched chamber, and then 10 μL sLex-AP (in Dulbecco's Phosphate Buffered saline, DPBS) (Gibco, China) or anti-EpCAM was added, after 2 hours' incubation, CTC-BioT Chip was gently washed with PBS, 1 mL of cell suspensions (105 cells mL-1) was loaded, then placing in a cell incubator under the conditions of 37°C and 5% CO2 .

    Techniques: Chromatin Immunoprecipitation, Concentration Assay, Incubation, Standard Deviation

    (A-B) Schematic workflow of the CTC- BioT Chip. The biotinylated-sLeX-AP coated on HA/CTS substrate for HCC CTCs capture. (C) SEM image of HA/CTS substrate. (D) Conceptual illustration of HCC CTC interacting with sLeX-AP coated CTC- BioT Chip. (E) SEM image of HepG2 cell captured on sLeX-AP coated CTC- BioT Chip. (F) Conceptual illustration of the molecular mechanism of capturing HCC CTC by sLeX-AP coated CTC- BioT Chip. Abbreviations: CTCs, circulating tumor cells; SEM, scanning electron microscope; HA/CTS, hydroxyapatite/chitosan.

    Journal: Theranostics

    Article Title: Aptamer-Mediated Transparent-Biocompatible Nanostructured Surfaces for Hepotocellular Circulating Tumor Cells Enrichment

    doi: 10.7150/thno.15284

    Figure Lengend Snippet: (A-B) Schematic workflow of the CTC- BioT Chip. The biotinylated-sLeX-AP coated on HA/CTS substrate for HCC CTCs capture. (C) SEM image of HA/CTS substrate. (D) Conceptual illustration of HCC CTC interacting with sLeX-AP coated CTC- BioT Chip. (E) SEM image of HepG2 cell captured on sLeX-AP coated CTC- BioT Chip. (F) Conceptual illustration of the molecular mechanism of capturing HCC CTC by sLeX-AP coated CTC- BioT Chip. Abbreviations: CTCs, circulating tumor cells; SEM, scanning electron microscope; HA/CTS, hydroxyapatite/chitosan.

    Article Snippet: Cell capture and identification Prior to cell capture, CTC-BioT Chip was placed into a size-matched chamber, and then 10 μL sLex-AP (in Dulbecco's Phosphate Buffered saline, DPBS) (Gibco, China) or anti-EpCAM was added, after 2 hours' incubation, CTC-BioT Chip was gently washed with PBS, 1 mL of cell suspensions (105 cells mL-1) was loaded, then placing in a cell incubator under the conditions of 37°C and 5% CO2 .

    Techniques: Chromatin Immunoprecipitation, Capture-C, Microscopy

    (A-B) Five control studies and their corresponding fluorescent micrographs (DAPI nuclear staining only): 1) sLex-AP coated CTC- BioT Chip, 2) random DNA coated CTC- BioT Chip, 3) SA coated CTC- BioT Chip, 4) bare CTC- BioT Chip, 5)sLeX-AP coated glass were examined. (C) Cell capture efficiences on sLeX-AP and anti-EpCAM coated CTC- BioT Chip were validated using HepG2 (EpCAM-), MCF7 (EpCAM+), HCT116 (EpCAM+), MGC803 (EpCAM+), HeLa (EpCAM-). (D)The captured cell number of CTCs was validated from two kinds of artificial blood samples. (E)The fluorescent micrographs of cancer cells captured from the artificial blood samples. Three-color immunocytochemistry method based on FITC-labeled anti-CD45, PE-labeled anti-CK, and DAPI nuclear staining was applied to identify and enumerate CTCs from non-specially trapped WBCs. Scale bars are 20μm. All capture efficiency experiments were under optimal condition. The error bar represents standard deviation from three repeats. Abbreviations: CTCs, circulating tumor cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin; DAPI, 4 ,6-diamidino-2-phenylindole dihydrochloride; WBCs, white blood cells.

    Journal: Theranostics

    Article Title: Aptamer-Mediated Transparent-Biocompatible Nanostructured Surfaces for Hepotocellular Circulating Tumor Cells Enrichment

    doi: 10.7150/thno.15284

    Figure Lengend Snippet: (A-B) Five control studies and their corresponding fluorescent micrographs (DAPI nuclear staining only): 1) sLex-AP coated CTC- BioT Chip, 2) random DNA coated CTC- BioT Chip, 3) SA coated CTC- BioT Chip, 4) bare CTC- BioT Chip, 5)sLeX-AP coated glass were examined. (C) Cell capture efficiences on sLeX-AP and anti-EpCAM coated CTC- BioT Chip were validated using HepG2 (EpCAM-), MCF7 (EpCAM+), HCT116 (EpCAM+), MGC803 (EpCAM+), HeLa (EpCAM-). (D)The captured cell number of CTCs was validated from two kinds of artificial blood samples. (E)The fluorescent micrographs of cancer cells captured from the artificial blood samples. Three-color immunocytochemistry method based on FITC-labeled anti-CD45, PE-labeled anti-CK, and DAPI nuclear staining was applied to identify and enumerate CTCs from non-specially trapped WBCs. Scale bars are 20μm. All capture efficiency experiments were under optimal condition. The error bar represents standard deviation from three repeats. Abbreviations: CTCs, circulating tumor cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin; DAPI, 4 ,6-diamidino-2-phenylindole dihydrochloride; WBCs, white blood cells.

    Article Snippet: Cell capture and identification Prior to cell capture, CTC-BioT Chip was placed into a size-matched chamber, and then 10 μL sLex-AP (in Dulbecco's Phosphate Buffered saline, DPBS) (Gibco, China) or anti-EpCAM was added, after 2 hours' incubation, CTC-BioT Chip was gently washed with PBS, 1 mL of cell suspensions (105 cells mL-1) was loaded, then placing in a cell incubator under the conditions of 37°C and 5% CO2 .

    Techniques: Staining, Chromatin Immunoprecipitation, Immunocytochemistry, Labeling, Standard Deviation

    Schematic illustration of coprecipitating LN within biomimetic apatite. Abbreviations: LN, laminin; mDPBS, modified Dulbecco’s phosphate-buffered saline.

    Journal: International Journal of Nanomedicine

    Article Title: Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells

    doi: 10.2147/IJN.S176596

    Figure Lengend Snippet: Schematic illustration of coprecipitating LN within biomimetic apatite. Abbreviations: LN, laminin; mDPBS, modified Dulbecco’s phosphate-buffered saline.

    Article Snippet: Materials Dulbecco’s phosphate-buffered saline (DPBS, without calcium and magnesium), phosphate-buffered saline (PBS), accutase cell dissociation reagent, micro BCA protein assay kit (mBCA), serum albumin and live/dead viability/cytotoxicity kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Modification

    Effect of pro-inflammatory cytokines on the expression of human leukocyte antigens (HLAs) and co-stimulatory molecules on the surface of ADSCs. ADSCs were isolated from human adipose tissue and cultured in complete Dulbecco’s modified Eagle’s medium (DMEM). ADSCs that had been passaged fewer than eight times were used. ADSCs were stained with monoclonal antibodies (mAbs) against CD80 and CD86. ADSCs were additionally stained with mAbs against HLA-DR and HLA-ABC and for markers of NKG2DL with mAbs against MIC-A, MIC-B, ULBP1 and ULBP2/5/6. The data are representative of at least three experiments. IFN+IL-17: combination of IFN-γ and IL-17A/F; IFN+IL-17+IL-23: combination of IFN-γ, IL-17A/F and IL-23.

    Journal: Cells

    Article Title: Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens

    doi: 10.3390/cells9051246

    Figure Lengend Snippet: Effect of pro-inflammatory cytokines on the expression of human leukocyte antigens (HLAs) and co-stimulatory molecules on the surface of ADSCs. ADSCs were isolated from human adipose tissue and cultured in complete Dulbecco’s modified Eagle’s medium (DMEM). ADSCs that had been passaged fewer than eight times were used. ADSCs were stained with monoclonal antibodies (mAbs) against CD80 and CD86. ADSCs were additionally stained with mAbs against HLA-DR and HLA-ABC and for markers of NKG2DL with mAbs against MIC-A, MIC-B, ULBP1 and ULBP2/5/6. The data are representative of at least three experiments. IFN+IL-17: combination of IFN-γ and IL-17A/F; IFN+IL-17+IL-23: combination of IFN-γ, IL-17A/F and IL-23.

    Article Snippet: Briefly, adipose tissue was finely cut with scissors and separated into adipocytes through digestion for 1 h in a water bath of 37 °C in Dulbecco’s phosphate-buffered saline (DPBS, Life Technologies, Grand Island, NY, USA) containing 0.1% collagenase type I (Life Technologies, Grand Island, NY, USA) [ ].

    Techniques: Expressing, Isolation, Cell Culture, Modification, Staining

    Absence of the analgesic effect following the injection of ASC-derived culture medium on the rat model with L5 spinal nerve ligation. (a, b) No effects observed on mechanical allodynia (a) and cold allodynia (b) by treatment with ASC-derived culture medium after the L5 spinal nerve ligation surgery. Arrows indicate time of injection (POD 14, POD 15). p > 0.05, compared with vehicle (30 μ l DMEM); n = 5 − 7 rats/group. p > 0.05, compared with POD14. All data are expressed as the mean ± SEM. Statistical significance was determined by 2-way ANOVA, followed by Sidak's posthoc test. ns: not significant; POD: postoperative day; BL: baseline; i.t.: intrathecal injection; ASC: adipose tissue-derived stem cell; DMEM: Dulbecco's modified Eagle's high-glucose medium.

    Journal: Stem Cells International

    Article Title: Adipose Tissue-Derived Stem Cells Alleviate Cold Allodynia in a Rat Spinal Nerve Ligation Model of Neuropathic Pain

    doi: 10.1155/2020/8845262

    Figure Lengend Snippet: Absence of the analgesic effect following the injection of ASC-derived culture medium on the rat model with L5 spinal nerve ligation. (a, b) No effects observed on mechanical allodynia (a) and cold allodynia (b) by treatment with ASC-derived culture medium after the L5 spinal nerve ligation surgery. Arrows indicate time of injection (POD 14, POD 15). p > 0.05, compared with vehicle (30 μ l DMEM); n = 5 − 7 rats/group. p > 0.05, compared with POD14. All data are expressed as the mean ± SEM. Statistical significance was determined by 2-way ANOVA, followed by Sidak's posthoc test. ns: not significant; POD: postoperative day; BL: baseline; i.t.: intrathecal injection; ASC: adipose tissue-derived stem cell; DMEM: Dulbecco's modified Eagle's high-glucose medium.

    Article Snippet: ReagentsWe used Dulbecco's phosphate-buffered saline (DPBS; Gibco, USA), penicillin streptomycin (Sigma, USA), collagenase-IV (Sigma, USA), 0.25% trypsin/1 mM ethylenediaminetetraacetic acid (Gibco-BRL, USA), Dulbecco's modified Eagle's high-glucose medium (DMEM, 4.5 g/L glucose; Gibco-BRL, USA), and fetal bovine serum (FBS; Gibco-BRL, USA).

    Techniques: Injection, Derivative Assay, Ligation, Modification

    Absence of analgesic effect following the retro -orbital injection of ASCs on the rat model with L5 spinal nerve ligation. (a, b) No effect observed on cold allodynia (a) and mechanical allodynia (b) by treatment with ASCs (1 × 10 6 cells) after the L5 spinal nerve ligation surgery. Arrows indicate time of injection (POD 0, POD 6, POD 13). p > 0.05, compared with vehicle (20 μ l DMEM); p > 0.05, compared with n = 6 rats/group. All data are expressed as the mean ± SEM. Statistical significance was determined by 2-way ANOVA, followed by Sidak's posthoc test. ns: not significant; POD: postoperative day; BL: baseline; i.v. : intravenous injection; ASC: adipose tissue-derived stem cell; DMEM: Dulbecco's modified Eagle's high-glucose medium.

    Journal: Stem Cells International

    Article Title: Adipose Tissue-Derived Stem Cells Alleviate Cold Allodynia in a Rat Spinal Nerve Ligation Model of Neuropathic Pain

    doi: 10.1155/2020/8845262

    Figure Lengend Snippet: Absence of analgesic effect following the retro -orbital injection of ASCs on the rat model with L5 spinal nerve ligation. (a, b) No effect observed on cold allodynia (a) and mechanical allodynia (b) by treatment with ASCs (1 × 10 6 cells) after the L5 spinal nerve ligation surgery. Arrows indicate time of injection (POD 0, POD 6, POD 13). p > 0.05, compared with vehicle (20 μ l DMEM); p > 0.05, compared with n = 6 rats/group. All data are expressed as the mean ± SEM. Statistical significance was determined by 2-way ANOVA, followed by Sidak's posthoc test. ns: not significant; POD: postoperative day; BL: baseline; i.v. : intravenous injection; ASC: adipose tissue-derived stem cell; DMEM: Dulbecco's modified Eagle's high-glucose medium.

    Article Snippet: ReagentsWe used Dulbecco's phosphate-buffered saline (DPBS; Gibco, USA), penicillin streptomycin (Sigma, USA), collagenase-IV (Sigma, USA), 0.25% trypsin/1 mM ethylenediaminetetraacetic acid (Gibco-BRL, USA), Dulbecco's modified Eagle's high-glucose medium (DMEM, 4.5 g/L glucose; Gibco-BRL, USA), and fetal bovine serum (FBS; Gibco-BRL, USA).

    Techniques: Injection, Ligation, Derivative Assay, Modification