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Welgene inc dulbecco s modified eagle s medium
Dulbecco S Modified Eagle S Medium, supplied by Welgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dulbecco s modified eagle s medium - by Bioz Stars, 2021-03
86/100 stars

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Modification:

Article Title: Antioxidant, inhibition of α-glucosidase and suppression of nitric oxide production in LPS-induced murine macrophages by different fractions of Actinidia arguta stem
Article Snippet: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), Folin–Ciocalteu’s reagent, Trolox, gallic acid, and yeast α-glucosidase were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and phosphate buffered saline (PBS) were from WelGENE (Daegu, Republic of Korea). .. The authentic stem material of A. arguta was purchased from local herbal store (Gangwon, Republic of Korea) and deposited at Ildong Foodis (Chuncheon, South Korea) with batch No. IL-2012-0023.

Article Title: Puffing of Rehmannia glutinosa enhances anti-oxidant capacity and down-regulates IL-6 production in RAW 264.7 cells
Article Snippet: .. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic/antimycotic solution were purchased from Welgene (Gyeongsan, Korea). .. Lipopolysaccharide (LPS), phosphate-buffered saline (PBS), ABTS, 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH), 5-HMF, and ascorbic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA), while DPPH was purchased from Aladdin (Shanghai, China).

Article Title: “Two-Cell Assemblage” Assay: A Simple in vitro Method for Screening Hair Growth-Promoting Compounds
Article Snippet: .. In detail, the cultured hDP cells (passage 2 or 3; 5 × 103 cells/well) were combined in a single cell suspension and seeded in each well of the 96-well plate in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum and an antibiotic/antimycotic solution. ..

Article Title: Ethyl Acetate Fraction from Dendropanax morbifera Leaves Increases T Cell Growth by Upregulating NF-AT-Mediated IL-2 Secretion.
Article Snippet: .. Dendropanax morbifera Leveille (Araliaceae) is an endemic species that grows in Southwestern Korea and has been used as a folk medicine. .. Dendropanax morbifera Leveille (Araliaceae) is an endemic species that grows in Southwestern Korea and has been used as a folk medicine.

Article Title: Anthocyanins from Hibiscus syriacus L. Inhibit NLRP3 Inflammasome in BV2 Microglia Cells by Alleviating NF-κB- and ER Stress-Induced Ca2+ Accumulation and Mitochondrial ROS Production
Article Snippet: Reagents and Antibody LPS from Escherichia coli O111:B4, ATP, salubrinal, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), and Fluo-4 AM were obtained from Sigma (St. Louis, MO, USA). .. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and antibiotic mixture were obtained from WelGENE (Gyeongsan-si, Gyeongsangbuk-do, Republic of Korea). .. Antibodies against ASC (sc-514414, 22 kDa), pro-caspase-1 (sc-56036, 45 kDa), p50 (sc-8414, 50 kDa), p65 (sc-8008, 65 kDa), CHOP (sc-7351, 30 kDa), nucleolin (sc-8031, 110 kDa), β -actin (sc-69879, 43 kDa), and peroxidase-labeled anti-mouse immunoglobulins (sc-16102) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Article Title: Human β-defensin 2 plays a regulatory role in innate antiviral immunity and is capable of potentiating the induction of antigen-specific immunity
Article Snippet: We measured color development using reading the absorbance at 405 nm on an ELISA plate reader (SPECTROstar Nano, BMG Labtech, Ortenberg, Germany). .. Vero E6 cells were used to propagate MERS-CoV and were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Welgene) supplemented with 10% FBS (Gibco) at 37 °C in a humidified CO2 incubator. .. MERS-CoV was passed six times in Vero E6 cells and used to assess the neutralizing potential of each recombinant protein.

Article Title: Anti-Melanogenic Effect of Dendropanax morbiferus and Its Active Components via Protein Kinase A/Cyclic Adenosine Monophosphate-Responsive Binding Protein- and p38 Mitogen-Activated Protein Kinase-Mediated Microphthalmia−Associated Transcription Factor Downregulation
Article Snippet: Chemical and Reagents L-3,4-dihydroxyphenylalanine (L-DOPA), α-melanocyte stimulating hormone (α-MSH), and arbutin were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). .. Dulbecco's modified Eagle's medium (DMEM) was obtained from Welgene (DG, KR). .. Fetal bovine serum (FBS) and penicillin/streptomycin were purchased from Thermo Fisher Scientific (MA, USA).

Article Title: Puffing as a Novel Process to Enhance the Antioxidant and Anti-Inflammatory Properties of Curcuma longa L. (Turmeric)
Article Snippet: .. RAW264.7 Cell Culture Murine macrophage RAW264.7 cells were cultured at 1 × 105 cells/well on 24-well plates in Dulbecco’s modified Eagle’s medium (DMEM, Welgene, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS, Welgene) and 1% antibiotic/antimycotic solution (10,000 U/mL penicillin G, 10,000 μg/mL streptomycin, and 25 μg/mL amphotericin B, Welgene) at 37 °C in a 5 % CO2 incubator (Model BB15, Thermo Scientific, Waltham, MA, USA). .. The cells were grown with or without turmeric extract for 24 h, and inflammatory responses were stimulated using 500 ng/mL lipopolysaccharide (LPS, Sigma-Aldrich Co., St. Louis, MO, USA) for 12 h.

Cell Culture:

Article Title: “Two-Cell Assemblage” Assay: A Simple in vitro Method for Screening Hair Growth-Promoting Compounds
Article Snippet: .. In detail, the cultured hDP cells (passage 2 or 3; 5 × 103 cells/well) were combined in a single cell suspension and seeded in each well of the 96-well plate in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum and an antibiotic/antimycotic solution. ..

Article Title: Puffing as a Novel Process to Enhance the Antioxidant and Anti-Inflammatory Properties of Curcuma longa L. (Turmeric)
Article Snippet: .. RAW264.7 Cell Culture Murine macrophage RAW264.7 cells were cultured at 1 × 105 cells/well on 24-well plates in Dulbecco’s modified Eagle’s medium (DMEM, Welgene, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS, Welgene) and 1% antibiotic/antimycotic solution (10,000 U/mL penicillin G, 10,000 μg/mL streptomycin, and 25 μg/mL amphotericin B, Welgene) at 37 °C in a 5 % CO2 incubator (Model BB15, Thermo Scientific, Waltham, MA, USA). .. The cells were grown with or without turmeric extract for 24 h, and inflammatory responses were stimulated using 500 ng/mL lipopolysaccharide (LPS, Sigma-Aldrich Co., St. Louis, MO, USA) for 12 h.

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    Welgene inc dmem
    SEM images, X-ray powder diffraction patterns and Miller indices of Cu 2 O crystals (A) and CuO crystals (B). Cu 2 O induces cell death, but does not activate caspase-3 (C). <t>BAECs</t> were grown to confluence in <t>DMEM</t> containing 20% serum and then serum-starved for 12 h before treating with the indicated concentrations of Cu 2 O or CuO crystals for the indicated periods of time. Cells were then observed under a microscope (Top panels). Dead cells (round, shrunken cells) counted per visual field were plotted on bar graphs in the bottom panels (means ± S.E., n = 3). * P
    Dmem, supplied by Welgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem/product/Welgene inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Welgene inc dulbecco s modified eagle s medium dmem
    Effects of LPLB5 and L. plantarum ATCC 8014 (LP8014) on the mRNA expression levels of ( a – c ) pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and ( d – f ) anti-inflammatory cytokines (IL-4, IL-10, and IFN-γ) in LPS-stimulated Caco-2 cells. Cells were pretreated with <t>Dulbecco’s</t> modified Eagle medium <t>(DMEM),</t> LPLB5, or L. plantarum ATCC 8014 (10 6 and 10 7 CFU/mL) for 6 h and then stimulated with LPS for 2 h. The results represent the mean ± SEM, with n = 3. * indicates a significant difference vs. control (* p
    Dulbecco S Modified Eagle S Medium Dmem, supplied by Welgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium dmem/product/Welgene inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium dmem - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    SEM images, X-ray powder diffraction patterns and Miller indices of Cu 2 O crystals (A) and CuO crystals (B). Cu 2 O induces cell death, but does not activate caspase-3 (C). BAECs were grown to confluence in DMEM containing 20% serum and then serum-starved for 12 h before treating with the indicated concentrations of Cu 2 O or CuO crystals for the indicated periods of time. Cells were then observed under a microscope (Top panels). Dead cells (round, shrunken cells) counted per visual field were plotted on bar graphs in the bottom panels (means ± S.E., n = 3). * P

    Journal: Molecules and Cells

    Article Title: Copper Ion from Cu2O Crystal Induces AMPK-Mediated Autophagy via Superoxide in Endothelial Cells

    doi: 10.14348/molcells.2016.2198

    Figure Lengend Snippet: SEM images, X-ray powder diffraction patterns and Miller indices of Cu 2 O crystals (A) and CuO crystals (B). Cu 2 O induces cell death, but does not activate caspase-3 (C). BAECs were grown to confluence in DMEM containing 20% serum and then serum-starved for 12 h before treating with the indicated concentrations of Cu 2 O or CuO crystals for the indicated periods of time. Cells were then observed under a microscope (Top panels). Dead cells (round, shrunken cells) counted per visual field were plotted on bar graphs in the bottom panels (means ± S.E., n = 3). * P

    Article Snippet: Cell culture Bovine aortic endothelial cells (BAECs) obtained from descending thoracic aortas were cultured in DMEM (1 g/L glucose; Wel GENE Inc, Korea) containing 20% fetal bovine serum (FBS, Wel GENE Inc) with antibiotics (50 μg/ml of penicillin/streptomycin) at 37°C with 5% CO2 .

    Techniques: Microscopy

    SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells with the absence or presence of ROT (0.5 μ M) for 48 h were treated with hBMSC-CM (50%) or NI-hBMSC-CM (50%) during the last 24 h and analyzed for NF-H (a), β 3-tubulin (b), NeuN (c), SYP (d), and GAPDH or β -actin by Western blotting. Each picture is representative of three independent experiments. Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Journal: Stem Cells International

    Article Title: Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis

    doi: 10.1155/2021/6658271

    Figure Lengend Snippet: SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells with the absence or presence of ROT (0.5 μ M) for 48 h were treated with hBMSC-CM (50%) or NI-hBMSC-CM (50%) during the last 24 h and analyzed for NF-H (a), β 3-tubulin (b), NeuN (c), SYP (d), and GAPDH or β -actin by Western blotting. Each picture is representative of three independent experiments. Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Article Snippet: SH-SY5Y Cell Culture and Rotenone Preparation The human neuroblastoma cell line SH-SY5Y (RRID: CVCL_0019; ATCC® CRL-2266) was maintained in DMEM (Welgene Inc., Gyeonsangbuk-do, Republic of Korea) supplemented with 10% FBS and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO2 /95% air as previously described [ ].

    Techniques: Incubation, Western Blot

    SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells with the absence or presence of ROT (0.5 μ M) for 48 h were treated with hBMSC-CM (50%) or NI-hBMSC-CM (50%) during the last 24 h and assessed for Bax (a), Bcl-2 (b), Bax/Bcl-2 ratio (c), Mcl-1 (d), and β -actin by Western blotting. Each picture is representative of three independent experiments. Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Journal: Stem Cells International

    Article Title: Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis

    doi: 10.1155/2021/6658271

    Figure Lengend Snippet: SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells with the absence or presence of ROT (0.5 μ M) for 48 h were treated with hBMSC-CM (50%) or NI-hBMSC-CM (50%) during the last 24 h and assessed for Bax (a), Bcl-2 (b), Bax/Bcl-2 ratio (c), Mcl-1 (d), and β -actin by Western blotting. Each picture is representative of three independent experiments. Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Article Snippet: SH-SY5Y Cell Culture and Rotenone Preparation The human neuroblastoma cell line SH-SY5Y (RRID: CVCL_0019; ATCC® CRL-2266) was maintained in DMEM (Welgene Inc., Gyeonsangbuk-do, Republic of Korea) supplemented with 10% FBS and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO2 /95% air as previously described [ ].

    Techniques: Incubation, Western Blot

    SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells incubated with the absence or presence of ROT (0.5 μ M) for 48 h were treated with NI-hBMSC-CM (50%) during the last 24 h. Cell lysates were prepared as 1% Triton X-100-soluble and Triton X-100-insoluble (2x SDS soluble) fractions. p-S129 and total α -syn were analyzed from 1% Triton X-100-soluble fractions by Western blotting using 12% and 8% SDS-PAGE gels (a). Each picture is representative of three independent experiments. Bar graphs represent fold changes in p-S129 α -syn/GAPDH (b, d) and total α -syn/GAPDH (c, e) in SDS-PAGE gels of 12% (b, c) or 8% (d, e). Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Journal: Stem Cells International

    Article Title: Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis

    doi: 10.1155/2021/6658271

    Figure Lengend Snippet: SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells incubated with the absence or presence of ROT (0.5 μ M) for 48 h were treated with NI-hBMSC-CM (50%) during the last 24 h. Cell lysates were prepared as 1% Triton X-100-soluble and Triton X-100-insoluble (2x SDS soluble) fractions. p-S129 and total α -syn were analyzed from 1% Triton X-100-soluble fractions by Western blotting using 12% and 8% SDS-PAGE gels (a). Each picture is representative of three independent experiments. Bar graphs represent fold changes in p-S129 α -syn/GAPDH (b, d) and total α -syn/GAPDH (c, e) in SDS-PAGE gels of 12% (b, c) or 8% (d, e). Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Article Snippet: SH-SY5Y Cell Culture and Rotenone Preparation The human neuroblastoma cell line SH-SY5Y (RRID: CVCL_0019; ATCC® CRL-2266) was maintained in DMEM (Welgene Inc., Gyeonsangbuk-do, Republic of Korea) supplemented with 10% FBS and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO2 /95% air as previously described [ ].

    Techniques: Incubation, Western Blot, SDS Page

    SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells incubated with the absence or presence of ROT (0.5 μ M) for 48 h were treated with NI-hBMSC-CM (50%) during the last 24 h. Cell lysates were prepared as 1% Triton X-100-soluble and Triton X-100-insoluble (2x SDS soluble) fractions. p-S129 and total α -syn were analyzed from 1% Triton X-100-insoluble (2x SDS soluble) fractions by Western blotting using 12% and 8% SDS-PAGE gels (a). Each picture is representative of three independent experiments. Bar graphs represent fold changes in p-S129 α -syn/GAPDH (b, d) and total α -syn/GAPDH (c, e) in SDS-PAGE gels of 12% (b, c) or 8% (d, e). Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Journal: Stem Cells International

    Article Title: Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis

    doi: 10.1155/2021/6658271

    Figure Lengend Snippet: SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells incubated with the absence or presence of ROT (0.5 μ M) for 48 h were treated with NI-hBMSC-CM (50%) during the last 24 h. Cell lysates were prepared as 1% Triton X-100-soluble and Triton X-100-insoluble (2x SDS soluble) fractions. p-S129 and total α -syn were analyzed from 1% Triton X-100-insoluble (2x SDS soluble) fractions by Western blotting using 12% and 8% SDS-PAGE gels (a). Each picture is representative of three independent experiments. Bar graphs represent fold changes in p-S129 α -syn/GAPDH (b, d) and total α -syn/GAPDH (c, e) in SDS-PAGE gels of 12% (b, c) or 8% (d, e). Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Article Snippet: SH-SY5Y Cell Culture and Rotenone Preparation The human neuroblastoma cell line SH-SY5Y (RRID: CVCL_0019; ATCC® CRL-2266) was maintained in DMEM (Welgene Inc., Gyeonsangbuk-do, Republic of Korea) supplemented with 10% FBS and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO2 /95% air as previously described [ ].

    Techniques: Incubation, Western Blot, SDS Page

    SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells with the absence or presence of ROT (0.5 μ M) for 48 h were treated with hBMSC-CM (50%) or NI-hBMSC-CM (50%) during the last 24 h and assessed for pro-Cas-9 (a), pro-Cas-3 (b), pro-Cas-7 (c), PARP (d), and β -actin or GAPDH by Western blotting. Each picture is representative of three independent experiments. Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Journal: Stem Cells International

    Article Title: Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis

    doi: 10.1155/2021/6658271

    Figure Lengend Snippet: SH-SY5Y cells were seeded as 5 × 10 4 cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells with the absence or presence of ROT (0.5 μ M) for 48 h were treated with hBMSC-CM (50%) or NI-hBMSC-CM (50%) during the last 24 h and assessed for pro-Cas-9 (a), pro-Cas-3 (b), pro-Cas-7 (c), PARP (d), and β -actin or GAPDH by Western blotting. Each picture is representative of three independent experiments. Data are the mean ± SEM of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. Statistical significance: a compared with control; b compared with ROT; ∗ p

    Article Snippet: SH-SY5Y Cell Culture and Rotenone Preparation The human neuroblastoma cell line SH-SY5Y (RRID: CVCL_0019; ATCC® CRL-2266) was maintained in DMEM (Welgene Inc., Gyeonsangbuk-do, Republic of Korea) supplemented with 10% FBS and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO2 /95% air as previously described [ ].

    Techniques: Incubation, Western Blot

    Inhibition of myogenic differentiation by Setdb1 depletion. (A) levels of Setdb1 decrease during C2C12 myoblast differentiation. C2C12 myoblast cells were grown to confluency in DMEM supplemented with 10% fetal bovine serum and differentiation was induced by serum withdrawal. Cells were harvested at the indicated time points and total RNAs or total protein extracts were prepared as described in “Materials and Methods”. RNA was analyzed by quantitative real-time RT-PCR using primers specific for Setdb1 and GAPDH. Relative expression of Setdb1 was determined using the standard curve method and then normalized to GAPDH. Error bars indicate standard deviation (left). Proteins were resolved on 7.5% (for Setdb1 and MHC) or 12% (for MyoD, myogenin, and Actin) SDS-PAGE and detected with antibodies against indicated proteins (right). (B-E) C2C12 myoblast cells with Setdb1 shRNA displayed severely delayed differentiation under serum-deprived conditions. C2C12 myoblast cells stably expressing control vector (pLKO.1) or Setdb1 shRNA were maintained in DMEM containing 10% fetal bovine serum and differentiation was initiated as described in Materials and methods. After 72 h, differentiation was assessed by the appearance of myotubes using photomicrograph (B), expression of MHC as well as endogenous MyoD using Western blot analysis (C), and number of MHC-positive nuclei per 10 3 cells using immunofluorescence (D, E).

    Journal: Molecules and Cells

    Article Title: Setdb1 Is Required for Myogenic Differentiation of C2C12 Myoblast Cells via Maintenance of MyoD Expression

    doi: 10.14348/molcells.2015.2291

    Figure Lengend Snippet: Inhibition of myogenic differentiation by Setdb1 depletion. (A) levels of Setdb1 decrease during C2C12 myoblast differentiation. C2C12 myoblast cells were grown to confluency in DMEM supplemented with 10% fetal bovine serum and differentiation was induced by serum withdrawal. Cells were harvested at the indicated time points and total RNAs or total protein extracts were prepared as described in “Materials and Methods”. RNA was analyzed by quantitative real-time RT-PCR using primers specific for Setdb1 and GAPDH. Relative expression of Setdb1 was determined using the standard curve method and then normalized to GAPDH. Error bars indicate standard deviation (left). Proteins were resolved on 7.5% (for Setdb1 and MHC) or 12% (for MyoD, myogenin, and Actin) SDS-PAGE and detected with antibodies against indicated proteins (right). (B-E) C2C12 myoblast cells with Setdb1 shRNA displayed severely delayed differentiation under serum-deprived conditions. C2C12 myoblast cells stably expressing control vector (pLKO.1) or Setdb1 shRNA were maintained in DMEM containing 10% fetal bovine serum and differentiation was initiated as described in Materials and methods. After 72 h, differentiation was assessed by the appearance of myotubes using photomicrograph (B), expression of MHC as well as endogenous MyoD using Western blot analysis (C), and number of MHC-positive nuclei per 10 3 cells using immunofluorescence (D, E).

    Article Snippet: Cell cultures, differentiation assays, and retroviral gene transfer Mouse C2C12 myoblast cells and C3H 10T1/2 mesenchymal cells were maintained in DMEM (WelGENE) supplemented with 10% fetal bovine serum (WelGENE) and antibiotics in humidified atmosphere with 5% CO2 at 37°C.

    Techniques: Inhibition, Quantitative RT-PCR, Expressing, Standard Deviation, SDS Page, shRNA, Stable Transfection, Plasmid Preparation, Western Blot, Immunofluorescence

    Effects of LPLB5 and L. plantarum ATCC 8014 (LP8014) on the mRNA expression levels of ( a – c ) pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and ( d – f ) anti-inflammatory cytokines (IL-4, IL-10, and IFN-γ) in LPS-stimulated Caco-2 cells. Cells were pretreated with Dulbecco’s modified Eagle medium (DMEM), LPLB5, or L. plantarum ATCC 8014 (10 6 and 10 7 CFU/mL) for 6 h and then stimulated with LPS for 2 h. The results represent the mean ± SEM, with n = 3. * indicates a significant difference vs. control (* p

    Journal: Foods

    Article Title: Probiotic Properties of Lactiplantibacillus plantarum LB5 Isolated from Kimchi Based on Nitrate Reducing Capability

    doi: 10.3390/foods9121777

    Figure Lengend Snippet: Effects of LPLB5 and L. plantarum ATCC 8014 (LP8014) on the mRNA expression levels of ( a – c ) pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and ( d – f ) anti-inflammatory cytokines (IL-4, IL-10, and IFN-γ) in LPS-stimulated Caco-2 cells. Cells were pretreated with Dulbecco’s modified Eagle medium (DMEM), LPLB5, or L. plantarum ATCC 8014 (10 6 and 10 7 CFU/mL) for 6 h and then stimulated with LPS for 2 h. The results represent the mean ± SEM, with n = 3. * indicates a significant difference vs. control (* p

    Article Snippet: Cell Culture and Treatments The human colon carcinoma cell line was supplied by the Korean Cell Line Bank (Jongno-gu, Seoul, Korea) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Welgene Inc., Gyeongsan-si, Korea) supplemented with 10% fetal bovine serum (FBS) (Welgene Inc., Gyeongsan-si, Korea) and 1% penicillin/streptomycin (v/v ) at 37 °C in a humidified atmosphere containing 5% CO2 .

    Techniques: Expressing, Modification