dulbecco s modified eagles  (Thermo Fisher)


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    Name:
    Dulbecco s Modified Eagle s Limiting Medium DMEM LM
    Description:
    Formulation Sterile filtered DMEM L leucine L methionine with 4 5g L glucose 4 0mM L glutamine sodium pyruvate and phenol red Related Products L Photo Leucine L Photo Methionine
    Catalog Number:
    30030
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Protein Biology|Protein Crosslinking|Protein Labeling & Crosslinking
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    Structured Review

    Thermo Fisher dulbecco s modified eagles
    Low-dose styrene maleic acid application effectively samples proteins from cells. ( a ) Labelling of cells with biotin prior to 6.25 ppm SMA application allowed confirmation with biotin immunoblotting of the presence of a range of proteins (20–200 kDa) in the obtained suspension. ( b ) Proteins were absent from the final post-biotinylation <t>Dulbecco’s</t> PBS cell wash, as confirmed across repetitions (ANOVA plus Tukey’s test * p
    Formulation Sterile filtered DMEM L leucine L methionine with 4 5g L glucose 4 0mM L glutamine sodium pyruvate and phenol red Related Products L Photo Leucine L Photo Methionine
    https://www.bioz.com/result/dulbecco s modified eagles/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagles - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Styrene maleic acid recovers proteins from mammalian cells and tissues while avoiding significant cell death"

    Article Title: Styrene maleic acid recovers proteins from mammalian cells and tissues while avoiding significant cell death

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-51896-1

    Low-dose styrene maleic acid application effectively samples proteins from cells. ( a ) Labelling of cells with biotin prior to 6.25 ppm SMA application allowed confirmation with biotin immunoblotting of the presence of a range of proteins (20–200 kDa) in the obtained suspension. ( b ) Proteins were absent from the final post-biotinylation Dulbecco’s PBS cell wash, as confirmed across repetitions (ANOVA plus Tukey’s test * p
    Figure Legend Snippet: Low-dose styrene maleic acid application effectively samples proteins from cells. ( a ) Labelling of cells with biotin prior to 6.25 ppm SMA application allowed confirmation with biotin immunoblotting of the presence of a range of proteins (20–200 kDa) in the obtained suspension. ( b ) Proteins were absent from the final post-biotinylation Dulbecco’s PBS cell wash, as confirmed across repetitions (ANOVA plus Tukey’s test * p

    Techniques Used:

    Related Articles

    Cell Culture:

    Article Title: HDAC6 Regulates the MRTF-A/SRF Axis and Vascular Smooth Muscle Cell Plasticity
    Article Snippet: In addition, our in vivo data show that a selective HDAC6 inhibitor (but not HDAC3 inhibitor) effectively reduced neointima in a rat vascular injury model. As such, the present study highlights the unique HDAC6 regulatory mechanism in SMC pathobiology that could be targeted in vivo for neointima mitigation. .. The mouse aortic smooth muscle MOVAS cell line was purchased from ATCC (Manassas, Virginia), and the cell culture was maintained at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, Massachusetts). ..

    Article Title: Enhanced Cardiomyogenic Differentiation of P19 Embryonal Carcinoma Stem Cells
    Article Snippet: Culture and cardiac differentiation of P19 cells The P19 cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco-BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco-BRL), 100 units of penicillin/mL, and 100 µg of streptomycin/mL. .. To induce cardiac differentiation, EB formation was induced by plating P19 cells on 10-cm bacterial dishes with 1×106 cells in 10 mL of DMEM supplemented with 1% DMSO (Sigma, St. Louis, MO, USA), 10% FBS, 100 units of penicillin/mL, and 100 µg of streptomycin/mL for 96 hours.

    Article Title: Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells
    Article Snippet: HepG2 cells and their derivatives are also used as a model system for studies of liver metabolism and toxicity of xenobiotics. .. HepG2 cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin, and 100 µg/ml streptomycin. .. For treatment with T3 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), cells were seeded in 12-well culture plates at a density of 3×105 cells/well, and maintained for 2 days incubated at 37°C in a humidified chamber containing 5% CO2 .

    Modification:

    Article Title: HDAC6 Regulates the MRTF-A/SRF Axis and Vascular Smooth Muscle Cell Plasticity
    Article Snippet: In addition, our in vivo data show that a selective HDAC6 inhibitor (but not HDAC3 inhibitor) effectively reduced neointima in a rat vascular injury model. As such, the present study highlights the unique HDAC6 regulatory mechanism in SMC pathobiology that could be targeted in vivo for neointima mitigation. .. The mouse aortic smooth muscle MOVAS cell line was purchased from ATCC (Manassas, Virginia), and the cell culture was maintained at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, Massachusetts). ..

    Article Title: Enhanced Cardiomyogenic Differentiation of P19 Embryonal Carcinoma Stem Cells
    Article Snippet: Culture and cardiac differentiation of P19 cells The P19 cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). .. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco-BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco-BRL), 100 units of penicillin/mL, and 100 µg of streptomycin/mL. .. To induce cardiac differentiation, EB formation was induced by plating P19 cells on 10-cm bacterial dishes with 1×106 cells in 10 mL of DMEM supplemented with 1% DMSO (Sigma, St. Louis, MO, USA), 10% FBS, 100 units of penicillin/mL, and 100 µg of streptomycin/mL for 96 hours.

    Article Title: Identification of Translational Activators of Glial Glutamate Transporter EAAT2 through Cell-Based High-Throughput Screening: An Approach to Prevent Excitotoxicity
    Article Snippet: PA-EAAT2 cells, a primary astrocyte line that stably expresses human EAAT2 transcripts with the 1091-nucleotides 5′ UTR driven by the cytomegalovirus (CMV) promoter, were generated in our previous study. .. PA-EAAT2 cells at passage 9 were thawed from frozen stocks and grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) containing 25 mM glucose, 1 mM sodium pyruvate, 19.4 μM pyridoxine hydrochloride, and 2 mM glutamine and supplemented with 10% fetal bovine serum (FBS), 700 μg/mL geneticin (Gibco, Los Angeles, CA), and 100 μg/mL penicillin-streptomycin (Sigma, St. Louis, MO). ..

    Article Title: Chemical Reactive Anchoring Lipids with Different Performance for Cell Surface Re-engineering Application
    Article Snippet: Azido–PEG4 –biotin and streptavidin-fluorescein isothiocyanate (streptavidin-FITC) were purchased from Biolegend (San Diego, CA). .. Dulbecco’s modified Eagle’s medium (DMEM), (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and MTT reagent were purchased from Life Technologies (Grand Island, NY). .. First, monocholesteryl–PEG2000 -amine was synthesized as per a previously described method.

    Article Title: Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells
    Article Snippet: HepG2 cells and their derivatives are also used as a model system for studies of liver metabolism and toxicity of xenobiotics. .. HepG2 cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin, and 100 µg/ml streptomycin. .. For treatment with T3 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), cells were seeded in 12-well culture plates at a density of 3×105 cells/well, and maintained for 2 days incubated at 37°C in a humidified chamber containing 5% CO2 .

    Article Title: Characterization of Virulent West Nile Virus Kunjin Strain, Australia, 2011
    Article Snippet: The mosquitoes were submitted live to the Medical Entomology Laboratory at Westmead Hospital (Westmead, NSW, Australia) for species identification, arbovirus isolation, and virus identification ( ). .. Cells and Viruses We propagated Vero 76 cells in Dulbecco modified minimum essential medium (DMEM; Life Technologies) supplemented with 10% fetal bovine serum (FBS). ..

    Article Title: Selenate Prevents Adipogenesis through Induction of Selenoprotein S and Attenuation of Endoplasmic Reticulum Stress
    Article Snippet: The fetal calf serum (FCS) and fetal bovine serum (FBS) were purchased from the PAA Cell Culture Company (Worcester, MA, USA). .. The Dulbecco’s Modified Eagle’s medium (DMEM) and 0.25% trypsin-EDTA were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. The rosiglitazone was purchased from Calbiochem (San Diego, CA, USA).

    Article Title: Mesenchymal Stromal Cells Engineered to Produce IGF-I by Recombinant Adenovirus Ameliorate Liver Fibrosis in Mice
    Article Snippet: Mononuclear cells were isolated using Ficoll–PaqueTM Plus density gradient (1.077 g/mL; GE Healthcare). .. Cells were plated at 4,000 cells per cm2 and incubated in Dulbecco's modified Eagle's medium low glucose (DMEM lg; Invitrogen/Life Technologies) supplemented with 10% fetal bovine serum (FBS; Gibco/Invitrogen). ..

    MTT Assay:

    Article Title: Chemical Reactive Anchoring Lipids with Different Performance for Cell Surface Re-engineering Application
    Article Snippet: Azido–PEG4 –biotin and streptavidin-fluorescein isothiocyanate (streptavidin-FITC) were purchased from Biolegend (San Diego, CA). .. Dulbecco’s modified Eagle’s medium (DMEM), (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and MTT reagent were purchased from Life Technologies (Grand Island, NY). .. First, monocholesteryl–PEG2000 -amine was synthesized as per a previously described method.

    Incubation:

    Article Title: Mesenchymal Stromal Cells Engineered to Produce IGF-I by Recombinant Adenovirus Ameliorate Liver Fibrosis in Mice
    Article Snippet: Mononuclear cells were isolated using Ficoll–PaqueTM Plus density gradient (1.077 g/mL; GE Healthcare). .. Cells were plated at 4,000 cells per cm2 and incubated in Dulbecco's modified Eagle's medium low glucose (DMEM lg; Invitrogen/Life Technologies) supplemented with 10% fetal bovine serum (FBS; Gibco/Invitrogen). ..

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  • 99
    Thermo Fisher glucose dmem
    Activator of thyroid and retinoid receptor (ACTR) enhances <t>sorafenib</t> resistance by affecting aerobic glycolysis in vitro. A and B, The relative viability curves of ACTR WT or KO HepG2 cells or ACTR KO HepG2 cells transiently transfected with ACTR, as well as Huh‐7 cells in <t>DMEM</t> with high glucose (25 mmol/L), transfected with ACTR siRNA or ACTR siRNA plus ACTR expression vector or non‐specific control for siRNA (Control siRNA); they were treated with Deoxy‐d‐glucose (2‐DG) (2.5 mmol/L) and increasing concentrations of sorafenib as indicated above. After 72 h, cell viability assays were performed using the CCK‐8. The group without treatment of sorafenib had 100% viable cells and was used as an internal control for comparison. The representative immunoblot with ACTR indicates ACTR expression levels. C and D, The relative viability curves of HepG2 cells (C) or Huh‐7 cells (D) transfected and treated as in (A) or (B) and cultured in DMEM with low glucose (5.5 mmol/L). E and F, Colony formation assays of HepG2 and Huh‐7 cells treated as in (A) and (B) with sorafenib (6 μmol/L) or not. G, Representative flow cytometry analysis of Annexin V (1:1000) and propidium iodide (1:1000) staining was carried out in HepG2 ACTR WT cells, KO cells, WT cells and KO cells treated with 2‐DG (2.5 mmol/L) and sorafenib (6 μmol/L) for 6 h. Data shown are mean ± SD of triplicate measurements that have been repeated three times with similar results. * P
    Glucose Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    97
    Thermo Fisher dmem
    Insulin does not induce activation of quiescent, freshly isolated mouse PaSC but promotes fibrosing responses. Freshly isolated mPaSC were cultured for 5 days in 1% FBS <t>DMEM/F12</t> media containing 5 mM or 25 mM glucose either alone or with 100 nM insulin.
    Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem - by Bioz Stars, 2021-06
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    99
    Thermo Fisher dulbecco s modified eagle s medium
    Validation of the enzyme-linked immunosorbent assay (ELISA). PA-EAAT2 cells were cultured in <t>Dulbecco’s</t> modified <t>Eagle’s</t> medium (DMEM) and treated with retinoic acid (1.5 μg/mL) for 72 h and then subjected to ( A ) ELISA, ( B ) Western
    Dulbecco S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Activator of thyroid and retinoid receptor (ACTR) enhances sorafenib resistance by affecting aerobic glycolysis in vitro. A and B, The relative viability curves of ACTR WT or KO HepG2 cells or ACTR KO HepG2 cells transiently transfected with ACTR, as well as Huh‐7 cells in DMEM with high glucose (25 mmol/L), transfected with ACTR siRNA or ACTR siRNA plus ACTR expression vector or non‐specific control for siRNA (Control siRNA); they were treated with Deoxy‐d‐glucose (2‐DG) (2.5 mmol/L) and increasing concentrations of sorafenib as indicated above. After 72 h, cell viability assays were performed using the CCK‐8. The group without treatment of sorafenib had 100% viable cells and was used as an internal control for comparison. The representative immunoblot with ACTR indicates ACTR expression levels. C and D, The relative viability curves of HepG2 cells (C) or Huh‐7 cells (D) transfected and treated as in (A) or (B) and cultured in DMEM with low glucose (5.5 mmol/L). E and F, Colony formation assays of HepG2 and Huh‐7 cells treated as in (A) and (B) with sorafenib (6 μmol/L) or not. G, Representative flow cytometry analysis of Annexin V (1:1000) and propidium iodide (1:1000) staining was carried out in HepG2 ACTR WT cells, KO cells, WT cells and KO cells treated with 2‐DG (2.5 mmol/L) and sorafenib (6 μmol/L) for 6 h. Data shown are mean ± SD of triplicate measurements that have been repeated three times with similar results. * P

    Journal: Cancer Science

    Article Title: Activator of thyroid and retinoid receptor increases sorafenib resistance in hepatocellular carcinoma by facilitating the Warburg effect, et al. Activator of thyroid and retinoid receptor increases sorafenib resistance in hepatocellular carcinoma by facilitating the Warburg effect

    doi: 10.1111/cas.14412

    Figure Lengend Snippet: Activator of thyroid and retinoid receptor (ACTR) enhances sorafenib resistance by affecting aerobic glycolysis in vitro. A and B, The relative viability curves of ACTR WT or KO HepG2 cells or ACTR KO HepG2 cells transiently transfected with ACTR, as well as Huh‐7 cells in DMEM with high glucose (25 mmol/L), transfected with ACTR siRNA or ACTR siRNA plus ACTR expression vector or non‐specific control for siRNA (Control siRNA); they were treated with Deoxy‐d‐glucose (2‐DG) (2.5 mmol/L) and increasing concentrations of sorafenib as indicated above. After 72 h, cell viability assays were performed using the CCK‐8. The group without treatment of sorafenib had 100% viable cells and was used as an internal control for comparison. The representative immunoblot with ACTR indicates ACTR expression levels. C and D, The relative viability curves of HepG2 cells (C) or Huh‐7 cells (D) transfected and treated as in (A) or (B) and cultured in DMEM with low glucose (5.5 mmol/L). E and F, Colony formation assays of HepG2 and Huh‐7 cells treated as in (A) and (B) with sorafenib (6 μmol/L) or not. G, Representative flow cytometry analysis of Annexin V (1:1000) and propidium iodide (1:1000) staining was carried out in HepG2 ACTR WT cells, KO cells, WT cells and KO cells treated with 2‐DG (2.5 mmol/L) and sorafenib (6 μmol/L) for 6 h. Data shown are mean ± SD of triplicate measurements that have been repeated three times with similar results. * P

    Article Snippet: At the same time, the sensitivity of low glucose DMEM (5.5 mM, Gibco) to sorafenib was detected.

    Techniques: In Vitro, Transfection, Expressing, Plasmid Preparation, CCK-8 Assay, Cell Culture, Flow Cytometry, Staining

    TSP1 immunodepletion or TSP1 knockout abolished ACM-exerted effects, which was rescued by the supplementation of exogenous TSP1 protein. ( a ) TSP1 antibodies were used for western blotting, which showed clear TSP1 bands in TSP1, mock, and ACM groups, a weak band in TSP1 immunodepleted ACM group (TSP1-ID-ACM), and no bands in empty, DMEM/F12, or TSP1 knockout ACM (TSP1-KO-ACM) groups. The image was cropped and inverted from a digital image captured by UVP imaging system (original images with ladder in the supplemental Fig. 1 ). ( b ) Immunofluorescence shows TUJ1-expressing connections between EGFP-ScNs (asterisks) and wild type CN neurons (arrowheads). A number of SV2-expressing puncta were observed along connections in the mock group (co-cultures supplied with the wild type ACM that has been immunodepleted by mouse IgG). The number of SV2-expressing puncta (arrows) along connections was reduced in the TSP1-ID-ACM group (co-cultures supplied with TSP1-ID-ACM), whereas it was increased when exogenous TSP1 protein (10 nM) was supplemented (the rescue group). ( b1 ) The quantitative study indicates that the relative number and area of SV2 puncta decreased in the TSP1-ID-ACM group, whereas they significantly increased in the rescue group (mean ± standard error shown in the figure; **indicates P

    Journal: Scientific Reports

    Article Title: Stimulation of synapse formation between stem cell-derived neurons and native brainstem auditory neurons

    doi: 10.1038/s41598-017-13764-8

    Figure Lengend Snippet: TSP1 immunodepletion or TSP1 knockout abolished ACM-exerted effects, which was rescued by the supplementation of exogenous TSP1 protein. ( a ) TSP1 antibodies were used for western blotting, which showed clear TSP1 bands in TSP1, mock, and ACM groups, a weak band in TSP1 immunodepleted ACM group (TSP1-ID-ACM), and no bands in empty, DMEM/F12, or TSP1 knockout ACM (TSP1-KO-ACM) groups. The image was cropped and inverted from a digital image captured by UVP imaging system (original images with ladder in the supplemental Fig. 1 ). ( b ) Immunofluorescence shows TUJ1-expressing connections between EGFP-ScNs (asterisks) and wild type CN neurons (arrowheads). A number of SV2-expressing puncta were observed along connections in the mock group (co-cultures supplied with the wild type ACM that has been immunodepleted by mouse IgG). The number of SV2-expressing puncta (arrows) along connections was reduced in the TSP1-ID-ACM group (co-cultures supplied with TSP1-ID-ACM), whereas it was increased when exogenous TSP1 protein (10 nM) was supplemented (the rescue group). ( b1 ) The quantitative study indicates that the relative number and area of SV2 puncta decreased in the TSP1-ID-ACM group, whereas they significantly increased in the rescue group (mean ± standard error shown in the figure; **indicates P

    Article Snippet: Dissociated CN tissues were transferred to culture wells containing 45% DMEM/F12, 45% neurobasal medium, 10% fetal bovine serum (FBS), 55 nM 2-mercaptoethanol, 0.1% penicillin/streptomycin (all from Invitrogen), and maintained in 5% CO2 incubator at 37 °C.

    Techniques: Knock-Out, Western Blot, Imaging, Immunofluorescence, Expressing

    Insulin does not induce activation of quiescent, freshly isolated mouse PaSC but promotes fibrosing responses. Freshly isolated mPaSC were cultured for 5 days in 1% FBS DMEM/F12 media containing 5 mM or 25 mM glucose either alone or with 100 nM insulin.

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Insulin promotes proliferation and fibrosing responses in activated pancreatic stellate cells

    doi: 10.1152/ajpgi.00251.2016

    Figure Lengend Snippet: Insulin does not induce activation of quiescent, freshly isolated mouse PaSC but promotes fibrosing responses. Freshly isolated mPaSC were cultured for 5 days in 1% FBS DMEM/F12 media containing 5 mM or 25 mM glucose either alone or with 100 nM insulin.

    Article Snippet: Cell culture DMEM/F12 medium (no. 11330-032), DMEM (no glucose; no. 11966-025), DMEM (25 mM glucose; no. 11965-092) and l -glutamine (no. 25030-081) were from ThermoFisher Scientific; antibiotics/antimycotics (1% Penicillin-Streptomycin; no. 25030-081) and fetal bovine serum (FBS; no. FB11) were from Omega Scientific (Tarzana, CA).

    Techniques: Activation Assay, Isolation, Cell Culture

    Quiescent and activated mouse PaSC express insulin (IR) and IGF (IGF-1R) receptors. A : freshly isolated, quiescent mouse PaSC (mPaSC) were cultured for 48 h ( day 3 ) or 96 h ( day 5 ) in DMEM/F12 medium (17.5 mM glucose) containing 10% FBS without or with

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Insulin promotes proliferation and fibrosing responses in activated pancreatic stellate cells

    doi: 10.1152/ajpgi.00251.2016

    Figure Lengend Snippet: Quiescent and activated mouse PaSC express insulin (IR) and IGF (IGF-1R) receptors. A : freshly isolated, quiescent mouse PaSC (mPaSC) were cultured for 48 h ( day 3 ) or 96 h ( day 5 ) in DMEM/F12 medium (17.5 mM glucose) containing 10% FBS without or with

    Article Snippet: Cell culture DMEM/F12 medium (no. 11330-032), DMEM (no glucose; no. 11966-025), DMEM (25 mM glucose; no. 11965-092) and l -glutamine (no. 25030-081) were from ThermoFisher Scientific; antibiotics/antimycotics (1% Penicillin-Streptomycin; no. 25030-081) and fetal bovine serum (FBS; no. FB11) were from Omega Scientific (Tarzana, CA).

    Techniques: Isolation, Cell Culture

    Validation of the enzyme-linked immunosorbent assay (ELISA). PA-EAAT2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and treated with retinoic acid (1.5 μg/mL) for 72 h and then subjected to ( A ) ELISA, ( B ) Western

    Journal: Journal of biomolecular screening

    Article Title: Identification of Translational Activators of Glial Glutamate Transporter EAAT2 through Cell-Based High-Throughput Screening: An Approach to Prevent Excitotoxicity

    doi: 10.1177/1087057110370998

    Figure Lengend Snippet: Validation of the enzyme-linked immunosorbent assay (ELISA). PA-EAAT2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and treated with retinoic acid (1.5 μg/mL) for 72 h and then subjected to ( A ) ELISA, ( B ) Western

    Article Snippet: PA-EAAT2 cells at passage 9 were thawed from frozen stocks and grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) containing 25 mM glucose, 1 mM sodium pyruvate, 19.4 μM pyridoxine hydrochloride, and 2 mM glutamine and supplemented with 10% fetal bovine serum (FBS), 700 μg/mL geneticin (Gibco, Los Angeles, CA), and 100 μg/mL penicillin-streptomycin (Sigma, St. Louis, MO).

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Modification, Western Blot

    Confirmation of the hits. PA-EAAT2 cells were treated with indicated concentrations of compound 9 in Dulbecco’s modified Eagle’s medium (DMEM) for 72 h and then harvested ( A ) for determining EAAT2 protein levels by Western blotting (equal

    Journal: Journal of biomolecular screening

    Article Title: Identification of Translational Activators of Glial Glutamate Transporter EAAT2 through Cell-Based High-Throughput Screening: An Approach to Prevent Excitotoxicity

    doi: 10.1177/1087057110370998

    Figure Lengend Snippet: Confirmation of the hits. PA-EAAT2 cells were treated with indicated concentrations of compound 9 in Dulbecco’s modified Eagle’s medium (DMEM) for 72 h and then harvested ( A ) for determining EAAT2 protein levels by Western blotting (equal

    Article Snippet: PA-EAAT2 cells at passage 9 were thawed from frozen stocks and grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) containing 25 mM glucose, 1 mM sodium pyruvate, 19.4 μM pyridoxine hydrochloride, and 2 mM glutamine and supplemented with 10% fetal bovine serum (FBS), 700 μg/mL geneticin (Gibco, Los Angeles, CA), and 100 μg/mL penicillin-streptomycin (Sigma, St. Louis, MO).

    Techniques: Modification, Western Blot