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Millipore dulbecco s modified eagle s medium
Dulbecco S Modified Eagle S Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dulbecco s modified eagle s medium - by Bioz Stars, 2021-03
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Modification:

Article Title: Length‐independent telomere damage drives post‐mitotic cardiomyocyte senescence
Article Snippet: .. The supernatant was then discarded and cells re‐suspended in cardiomyocyte growth medium (Dulbecco's Modified Eagle's Medium (DMEM), supplemented with 17% Medium 199, 5% FBS, 10% horse serum (SIGMA, H0146), 100 μg/ml streptomycin, 100 units/ml penicillin and 2 mM l‐glutamine). .. Cells were then seeded into a collagen‐coated (1 mg/ml) T75 culture flask (SIGMA) and incubated at 37°C for 2 h. After the incubation, the supernatant, containing cardiomyocytes, was collected from the flask and the adherent fibroblasts discarded.

Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells
Article Snippet: .. After overnight incubation (day 0), pellets were grown in a serum-free chondrogenic induction medium [high-glucose Dulbecco's modified Eagle's medium (DMEM) with 40 μg/ml proline (MilliporeSigma), 100 nM dexamethasone (MilliporeSigma), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM ascorbic acid-2-phosphate, and 1 × ITS™ Premix (BD Biosciences)] with the supplementation of 10 ng/ml transforming growth factor beta3 (TGFβ3; PeproTech, Rocky Hill, NJ) for up to 18 days. .. Chondrogenic differentiation was assessed using histology, immunohistochemistry, and qPCR.

Article Title: Effect of transplantation of olfactory ensheathing cell conditioned medium induced bone marrow stromal cells on rats with spinal cord injury
Article Snippet: .. The granular layer was washed twice with D-Hank's buffer, placed in a 37°C incubator, and digested with 0.125% trypsin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 25 min. Digestion was terminated with a trypsin terminator (0.2 mM/l; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 8 min at 37°C and the mixture was centrifuged at 71.6 × g for 5 min at 37°C, before it was washed once with serum-free Dulbecco's modified Eagle's medium (DMEM)/F12 medium (Sigma-Aldrich; Merck KGaA). .. Finally, single cell suspensions were produced using DMEM/F12 medium containing 20% fetal calf serum (FCS; Sigma-Aldrich; Merck KGaA), seeded in plastic culture flasks and cultured in an incubator at 37°C and 5% CO2 .

Article Title: Additive Effects of Zinc Chloride on the Suppression of Hepatitis A Virus Replication by Interferon in Human Hepatoma Huh7 Cells
Article Snippet: .. The human hepatoma cell lines Huh7 and HepG2 were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, Saint Louis, MO, USA) containing 10% fetal calf serum (FCS), 100 U/ml penicillin G and 200 μg/ml streptomycin at 37˚C in an atmosphere of 5% CO2 ( ). .. The HAV genotype IIIA HA11-1299 was established by Okamoto et al. and grown in cell culture as described previously ( ).

Article Title: Iron limitation promotes the atrophy of skeletal myocytes, whereas iron supplementation prevents this process in the hypoxic conditions
Article Snippet: Hypoxia was generated in a standard cell culture incubator by displacing O2 with infusion of N2 , which was supplied by an external high-pressure liquid nitrogen tank. .. Cell culture conditions L6 cells were grown in 37°C in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) with the addition of 10% fetal bovine serum (FBS; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 2 mmol/l glutamine, 1 U/ml penicillin and 10 mg/ml streptomycin (all from Sigma-Aldrich; Merck KGaA). .. For passages, cells were washed with PBS (without ions Ca2+ and Mg2+ ) and released via the use of Trypsin (Sigma-Aldrich; Merck KGaA).

Article Title: Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway
Article Snippet: .. Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypsin-EDTA and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Fibronectin was purchased from BD Biosciences (Franklin Lakes, NJ, USA) and Transwell chambers from Costar (Corning Inc., NY, USA).

Article Title: Involvement of Hydrogen Peroxide in the Differentiation of Clonal HD-11EM Cells Into Osteoclast-Like Cells
Article Snippet: The HD-11EM clonal cell line ( ) was kindly provided by Drs. Yi-Jan Hsia and Peter V. Hauschka (Children’s Hospital, Boston, MA), and was derived from the original HD-11 line of v- myc transformed chicken bone marrow cells ( ) obtained from Dr. John S. Adams (UCLA School of Medicine, Los Angeles, CA). .. Cells were maintained in 75-cm2 tissue culture flasks (Falcon Becton Dickinson Labware, Franklin Lanes, NJ) in Dulbecco’s modified Eagle’s medium (DMEM/F12; Sigma D-8900, Sigma) 200 ml supplemented with 1.5 ml penicillin-streptomycin (15070-014, Gibco BRL, Grand Island, NY), 0.1 ml of Fungizone (Gibco BRL 15295-017), 1.5 ml of l-glutamine (25030-016, Gibco BRL), and 10% heat-inactivated fetal calf serum (FCS) (Hyclone, Logan, UT) at 37°C in a humidified atmosphere of 5% CO2 and 95% air. .. Cells were passaged once a week before reaching confluency by transferring 0.3 ml of the 1 ml of trypsinized cells to 20 ml of fresh medium containing 10% heat-inactivated FCS, and were fed 2–3 days after passage by complete media replacement.

Incubation:

Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells
Article Snippet: .. After overnight incubation (day 0), pellets were grown in a serum-free chondrogenic induction medium [high-glucose Dulbecco's modified Eagle's medium (DMEM) with 40 μg/ml proline (MilliporeSigma), 100 nM dexamethasone (MilliporeSigma), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM ascorbic acid-2-phosphate, and 1 × ITS™ Premix (BD Biosciences)] with the supplementation of 10 ng/ml transforming growth factor beta3 (TGFβ3; PeproTech, Rocky Hill, NJ) for up to 18 days. .. Chondrogenic differentiation was assessed using histology, immunohistochemistry, and qPCR.

Derivative Assay:

Article Title: CRY1/2 selectively repress PPARδ and limit exercise capacity
Article Snippet: .. We performed fatty acid oxidation assays after incubating myotubes in substrate-limited media [glucose- and glutamine-free DMEM base media (Sigma #D5030) supplemented with 0.5 mM glucose, 1 mM glutamine, 0.5 mM carnitine and 1% heatin-activated horse serum] for 3–8 hours; it was difficult to achieve appropriate substrate-limited conditions such that fatty acid oxidation derived primarily from exogenous palmitate without impacting cell viability and this was variable between experiments; data shown represent one of six experiments performed with similar differences in overall palmitate oxidation between genotypes in which we achieved the best utilization of exogenous palmitate in all cells (e.g. very little utilization of endogenous palmitate) such that reliable calculations could be performed. ..

Cell Culture:

Article Title: Iron limitation promotes the atrophy of skeletal myocytes, whereas iron supplementation prevents this process in the hypoxic conditions
Article Snippet: Hypoxia was generated in a standard cell culture incubator by displacing O2 with infusion of N2 , which was supplied by an external high-pressure liquid nitrogen tank. .. Cell culture conditions L6 cells were grown in 37°C in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) with the addition of 10% fetal bovine serum (FBS; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 2 mmol/l glutamine, 1 U/ml penicillin and 10 mg/ml streptomycin (all from Sigma-Aldrich; Merck KGaA). .. For passages, cells were washed with PBS (without ions Ca2+ and Mg2+ ) and released via the use of Trypsin (Sigma-Aldrich; Merck KGaA).

MTT Assay:

Article Title: Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway
Article Snippet: .. Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypsin-EDTA and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Fibronectin was purchased from BD Biosciences (Franklin Lakes, NJ, USA) and Transwell chambers from Costar (Corning Inc., NY, USA).

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  • 97
    Millipore dmem cell culture medium
    Physical-chemical characterization of polystyrene NPs. Intensity weighted size distribution of carboxylated ( a ) and PEGylated ( b ) polystyrene nanoparticles measured by dynamic light scattering. Representative TEM images of the particles are shown in the top right panels of each DLS plot, scale bars represent 400 nm. c , d Aggregation–agglomeration properties and corona thickening of polystyrene nanoparticles was measured by DLS as a change in hydrodynamic size. Particles were incubated in inorganic ( c ) or biological solutions ( d ) for 96 h, and the size distribution was monitored. Color codes of samples are shown in the figure. Data are presented as mean ± standard deviation (n = 3). Particles showed no aggregation in distilled water or in <t>PBS</t> during the 96-h assay period ( c ). In contrast a time-dependent, heavy particle aggregation was found in serum-free <t>DMEM</t> (d). Incubation of nanoparticles with 10 % FBS also evoked an immediate size increase, but prevented large-scale aggregation ( d ). SDS-PAGE analysis confirmed the adsorption of serum proteins to both PS-COOH and PS-PEG nanoparticles after 1 h incubation in 10 % serum containing MEM (e) and verified reduced protein adsorption of PEG-coated nanoparticles after 24 h incubation ( f )
    Dmem Cell Culture Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem cell culture medium/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem cell culture medium - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Millipore dulbecco s modified eagle s medium ham s nutrient mixture f12
    Signaling assays. Aortic smooth muscle cells were isolated from Rgs5 +/+ and Rgs5 −/− mice and cultured in <t>Dulbecco's</t> modified <t>Eagle's</t> <t>medium-F12</t> containing 20% fetal calf serum. Cells were serum starved for 24 h
    Dulbecco S Modified Eagle S Medium Ham S Nutrient Mixture F12, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium ham s nutrient mixture f12/product/Millipore
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    98
    Millipore dmem cultured jpcs
    Quantification of MSCA-1 + cells under <t>DMEM-</t> and MC-XF culturing conditions by FACS analysis. Unseparated <t>JPCs</t> were plated in culture dishes in DMEM medium. For the first test runs, cells were maintained in DMEM medium whereas the second series underwent stepwise FCS reduction and convertion to MC-XF medium. At the same time points (day 2, 5 and 9 after conversion from DMEM to MC-XF culture conditions), cells were detached from the dishes and the percentages of MSCA-1 + cells were determined by FACS analysis. Significant higher amounts of MSCA-1 + cells were detected under MC-XF culturing conditions at day 2 and 5 (p
    Dmem Cultured Jpcs, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem cultured jpcs/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem cultured jpcs - by Bioz Stars, 2021-03
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    97
    Millipore serum free chondrogenic induction medium
    <t>Chondrogenic</t> potential of human IPFSCs after FN1-KO. Human IPFSCs were chondrogenically induced in a pellet culture system for 18 days. The effect of fibronectin on chondrogenic capacity of human IPFSCs was evaluated using gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs and immunohistochemical staining (IHC) for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P
    Serum Free Chondrogenic Induction Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Physical-chemical characterization of polystyrene NPs. Intensity weighted size distribution of carboxylated ( a ) and PEGylated ( b ) polystyrene nanoparticles measured by dynamic light scattering. Representative TEM images of the particles are shown in the top right panels of each DLS plot, scale bars represent 400 nm. c , d Aggregation–agglomeration properties and corona thickening of polystyrene nanoparticles was measured by DLS as a change in hydrodynamic size. Particles were incubated in inorganic ( c ) or biological solutions ( d ) for 96 h, and the size distribution was monitored. Color codes of samples are shown in the figure. Data are presented as mean ± standard deviation (n = 3). Particles showed no aggregation in distilled water or in PBS during the 96-h assay period ( c ). In contrast a time-dependent, heavy particle aggregation was found in serum-free DMEM (d). Incubation of nanoparticles with 10 % FBS also evoked an immediate size increase, but prevented large-scale aggregation ( d ). SDS-PAGE analysis confirmed the adsorption of serum proteins to both PS-COOH and PS-PEG nanoparticles after 1 h incubation in 10 % serum containing MEM (e) and verified reduced protein adsorption of PEG-coated nanoparticles after 24 h incubation ( f )

    Journal: Journal of Nanobiotechnology

    Article Title: Enhanced detection with spectral imaging fluorescence microscopy reveals tissue- and cell-type-specific compartmentalization of surface-modified polystyrene nanoparticles

    doi: 10.1186/s12951-016-0210-0

    Figure Lengend Snippet: Physical-chemical characterization of polystyrene NPs. Intensity weighted size distribution of carboxylated ( a ) and PEGylated ( b ) polystyrene nanoparticles measured by dynamic light scattering. Representative TEM images of the particles are shown in the top right panels of each DLS plot, scale bars represent 400 nm. c , d Aggregation–agglomeration properties and corona thickening of polystyrene nanoparticles was measured by DLS as a change in hydrodynamic size. Particles were incubated in inorganic ( c ) or biological solutions ( d ) for 96 h, and the size distribution was monitored. Color codes of samples are shown in the figure. Data are presented as mean ± standard deviation (n = 3). Particles showed no aggregation in distilled water or in PBS during the 96-h assay period ( c ). In contrast a time-dependent, heavy particle aggregation was found in serum-free DMEM (d). Incubation of nanoparticles with 10 % FBS also evoked an immediate size increase, but prevented large-scale aggregation ( d ). SDS-PAGE analysis confirmed the adsorption of serum proteins to both PS-COOH and PS-PEG nanoparticles after 1 h incubation in 10 % serum containing MEM (e) and verified reduced protein adsorption of PEG-coated nanoparticles after 24 h incubation ( f )

    Article Snippet: The suspensions were further diluted 1:10 with PBS (pH 7.4), DMEM cell culture medium (Sigma-Aldrich, St. Louis, MO, USA) or DMEM supplemented with 10 % fetal bovine serum (Invitrogen/Gibco, Carlsbad, CA, USA).

    Techniques: Transmission Electron Microscopy, Incubation, Standard Deviation, SDS Page, Adsorption

    Signaling assays. Aortic smooth muscle cells were isolated from Rgs5 +/+ and Rgs5 −/− mice and cultured in Dulbecco's modified Eagle's medium-F12 containing 20% fetal calf serum. Cells were serum starved for 24 h

    Journal:

    Article Title: Rgs5 Targeting Leads to Chronic Low Blood Pressure and a Lean Body Habitus ▿

    doi: 10.1128/MCB.01889-07

    Figure Lengend Snippet: Signaling assays. Aortic smooth muscle cells were isolated from Rgs5 +/+ and Rgs5 −/− mice and cultured in Dulbecco's modified Eagle's medium-F12 containing 20% fetal calf serum. Cells were serum starved for 24 h

    Article Snippet: Cells in culture were maintained in Dulbecco's modified Eagle's medium-F12 containing 20% fetal calf serum and 2 mM glutamine with penicillin and streptomycin and were serum starved for 24 h prior to the signaling assays.

    Techniques: Isolation, Mouse Assay, Cell Culture, Modification

    Quantification of MSCA-1 + cells under DMEM- and MC-XF culturing conditions by FACS analysis. Unseparated JPCs were plated in culture dishes in DMEM medium. For the first test runs, cells were maintained in DMEM medium whereas the second series underwent stepwise FCS reduction and convertion to MC-XF medium. At the same time points (day 2, 5 and 9 after conversion from DMEM to MC-XF culture conditions), cells were detached from the dishes and the percentages of MSCA-1 + cells were determined by FACS analysis. Significant higher amounts of MSCA-1 + cells were detected under MC-XF culturing conditions at day 2 and 5 (p

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Quantification of MSCA-1 + cells under DMEM- and MC-XF culturing conditions by FACS analysis. Unseparated JPCs were plated in culture dishes in DMEM medium. For the first test runs, cells were maintained in DMEM medium whereas the second series underwent stepwise FCS reduction and convertion to MC-XF medium. At the same time points (day 2, 5 and 9 after conversion from DMEM to MC-XF culture conditions), cells were detached from the dishes and the percentages of MSCA-1 + cells were determined by FACS analysis. Significant higher amounts of MSCA-1 + cells were detected under MC-XF culturing conditions at day 2 and 5 (p

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: FACS

    Detection of mineral deposition by mineralizing JPCs (of passage 6). The upper panel of the figure illustrates the beginning of mineralization at day 12 of osteogenic induction (ob) under both media conditions. Note that precipitate formation originates from only a few cells of the DMEM-cultured monolayer. In contrast, the MC-XF-cultivated monolayer seemed to be purer due to the appearance of mineralization potential originating from almost every cell of the monolayer. 4× magnification. The lower panel of the figure illustrates representative fluorescent stainings of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. JPCs cultivated under DMEM media conditions showed a stronger mineralization potential. 10× magnification.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Detection of mineral deposition by mineralizing JPCs (of passage 6). The upper panel of the figure illustrates the beginning of mineralization at day 12 of osteogenic induction (ob) under both media conditions. Note that precipitate formation originates from only a few cells of the DMEM-cultured monolayer. In contrast, the MC-XF-cultivated monolayer seemed to be purer due to the appearance of mineralization potential originating from almost every cell of the monolayer. 4× magnification. The lower panel of the figure illustrates representative fluorescent stainings of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. JPCs cultivated under DMEM media conditions showed a stronger mineralization potential. 10× magnification.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Cell Culture

    Expression patterns of DMEM- and MC-XF-cultured JPCs (growing within coated flasks) by flow cytometric analyses. Representative histograms and the average percentages (±STD) of positive cells for CD29, CD45, CD73, CD90 and CD105 expression by unseparated JPCs are illustrated.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Expression patterns of DMEM- and MC-XF-cultured JPCs (growing within coated flasks) by flow cytometric analyses. Representative histograms and the average percentages (±STD) of positive cells for CD29, CD45, CD73, CD90 and CD105 expression by unseparated JPCs are illustrated.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Expressing, Cell Culture, Flow Cytometry

    Detection of mineral deposition by non-mineralizing JPCs (of passage 6). Representative fluorescent staining of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. Non-mineralizing JPCs showed mineralization capacity only under MC-XF but not DMEM media conditions. 10× magnification. Co = untreated cell, ob = osteogenic induced cells.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Detection of mineral deposition by non-mineralizing JPCs (of passage 6). Representative fluorescent staining of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. Non-mineralizing JPCs showed mineralization capacity only under MC-XF but not DMEM media conditions. 10× magnification. Co = untreated cell, ob = osteogenic induced cells.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Staining, Cell Culture

    Quantitative analysis of gene expression levels in DMEM- and MC-XF-cultured JPCs at day 5 and 10 of osteogenesis (of passage 6, n = 4). Induction indices (x-fold) and significance values of alkaline phosphatase, Runx-2, type I collagen (alpha1-chain) and osteoprotegerin in osteogenic induced in comparison to untreated cells under both culture conditions are illustrated.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Quantitative analysis of gene expression levels in DMEM- and MC-XF-cultured JPCs at day 5 and 10 of osteogenesis (of passage 6, n = 4). Induction indices (x-fold) and significance values of alkaline phosphatase, Runx-2, type I collagen (alpha1-chain) and osteoprotegerin in osteogenic induced in comparison to untreated cells under both culture conditions are illustrated.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Expressing, Cell Culture

    Life-monitoring measurements of cell proliferation by unseparated JPCs using the x-CELLigence system (ACEA Biosciences). JPCs of passage 4 derived from two different donors were seeded into special E-plates in DMEM/F12/10%FCS culture medium. Two days later (44 hours - each tick of the scale corresponds to 11 hours), a gradual FCS reduction was performed in one-half of the test runs (green and dark green), whereas the other half of the wells was further cultivated in DMEM/F12/10%FCS (red and coral). Nine days (297 hours) after the initiation of FCS reduction, MC-XF culture medium was added to the cells. The proliferation curve progression of DMEM-cultured JPCs (from two representative patients) is highlighted in red and coral and that of MC-XF-cultivated cells is highlighted in dark green and green. The right panel of the figure shows the cell morphology of DMEM- and MC-XF-cultured JPCs. Note the reduced cell size under MC-XF culture conditions (on uncoated dishes) leading to the significant decrease of cell impedance immediately after the addition of the MC-XF culture medium.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Life-monitoring measurements of cell proliferation by unseparated JPCs using the x-CELLigence system (ACEA Biosciences). JPCs of passage 4 derived from two different donors were seeded into special E-plates in DMEM/F12/10%FCS culture medium. Two days later (44 hours - each tick of the scale corresponds to 11 hours), a gradual FCS reduction was performed in one-half of the test runs (green and dark green), whereas the other half of the wells was further cultivated in DMEM/F12/10%FCS (red and coral). Nine days (297 hours) after the initiation of FCS reduction, MC-XF culture medium was added to the cells. The proliferation curve progression of DMEM-cultured JPCs (from two representative patients) is highlighted in red and coral and that of MC-XF-cultivated cells is highlighted in dark green and green. The right panel of the figure shows the cell morphology of DMEM- and MC-XF-cultured JPCs. Note the reduced cell size under MC-XF culture conditions (on uncoated dishes) leading to the significant decrease of cell impedance immediately after the addition of the MC-XF culture medium.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Derivative Assay, Cell Culture

    Chondrogenic potential of human IPFSCs after FN1-KO. Human IPFSCs were chondrogenically induced in a pellet culture system for 18 days. The effect of fibronectin on chondrogenic capacity of human IPFSCs was evaluated using gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs and immunohistochemical staining (IHC) for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

    doi: 10.3389/fbioe.2019.00321

    Figure Lengend Snippet: Chondrogenic potential of human IPFSCs after FN1-KO. Human IPFSCs were chondrogenically induced in a pellet culture system for 18 days. The effect of fibronectin on chondrogenic capacity of human IPFSCs was evaluated using gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs and immunohistochemical staining (IHC) for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Article Snippet: After overnight incubation (day 0), pellets were grown in a serum-free chondrogenic induction medium [high-glucose Dulbecco's modified Eagle's medium (DMEM) with 40 μg/ml proline (MilliporeSigma), 100 nM dexamethasone (MilliporeSigma), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM ascorbic acid-2-phosphate, and 1 × ITS™ Premix (BD Biosciences)] with the supplementation of 10 ng/ml transforming growth factor beta3 (TGFβ3; PeproTech, Rocky Hill, NJ) for up to 18 days.

    Techniques: Staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Marker

    Chondrogenic potential of human IPFSCs after expansion on dECMs deposited by FN1-KO cells. Passage 15 human IPFSCs in a pellet culture system were compared for chondrogenic capacity after expansion on dECMs deposited by Cas9-sgFN1a/b transduced cells (sgFN1a ECM and sgFN1b ECM, respectively) with those deposited by copGFP (copGFP ECM) and those grown on TCP (TCP) as controls including gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs, and IHC for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

    doi: 10.3389/fbioe.2019.00321

    Figure Lengend Snippet: Chondrogenic potential of human IPFSCs after expansion on dECMs deposited by FN1-KO cells. Passage 15 human IPFSCs in a pellet culture system were compared for chondrogenic capacity after expansion on dECMs deposited by Cas9-sgFN1a/b transduced cells (sgFN1a ECM and sgFN1b ECM, respectively) with those deposited by copGFP (copGFP ECM) and those grown on TCP (TCP) as controls including gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs, and IHC for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Article Snippet: After overnight incubation (day 0), pellets were grown in a serum-free chondrogenic induction medium [high-glucose Dulbecco's modified Eagle's medium (DMEM) with 40 μg/ml proline (MilliporeSigma), 100 nM dexamethasone (MilliporeSigma), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM ascorbic acid-2-phosphate, and 1 × ITS™ Premix (BD Biosciences)] with the supplementation of 10 ng/ml transforming growth factor beta3 (TGFβ3; PeproTech, Rocky Hill, NJ) for up to 18 days.

    Techniques: Staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Marker