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Mediatech dulbecco s modified eagle s medium
Dulbecco S Modified Eagle S Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dulbecco s modified eagle s medium/product/Mediatech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dulbecco s modified eagle s medium - by Bioz Stars, 2021-03
86/100 stars

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Modification:

Article Title: Early Growth Response 1 (Egr1) Regulates Cholesterol Biosynthetic Gene Expression *
Article Snippet: .. H4IIE cells were maintained in Dulbecco's modification of Eagle's medium (DMEM) (Mediatech) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37 °C and 5% CO2 and passaged twice weekly. ..

Article Title: Myxoma Virus M083 Is a Virulence Factor Which Mediates Systemic Dissemination
Article Snippet: .. BSC40 and A549 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum (FBS) and 1× penicillin–streptomycin– l -glutamine (Mediatech, Inc., Manassas, VA, USA). .. RK13 cells were cultured in minimum essential medium (MEM) plus 10% fetal bovine serum and 1× penicillin–streptomycin– l -glutamine (Mediatech, Inc., Manassas, VA, USA).

Article Title: Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus *Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus * SLoss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus * S ◆
Article Snippet: .. After 24 or 48 h of treatment, the cell culture medium was removed, and an equal volume of a 1 mg/ml MTT solution, pH 7.4, in Dulbecco’s modified Eagle’s medium was added to the cells. .. After incubation at 37 °C for 1 h, the MTT solution was removed, and N -propyl alcohol was added to solubilize the formazan product whose absorbance was measured in a spectrophotometer at a wavelength of 560 nm.

Article Title: Heterometallic titanium-gold complexes inhibit renal cancer cells in vitro and in vivo ‡
Article Snippet: Human renal cell carcinoma lines A498, Caki-1 and UO31, as well as the human prostate carcinoma cell lines DU145 and PC3 were newly obtained for these studies from the American Type Culture Collection (ATCC) (Manassas, Virginia, USA) and cultured in Roswell Park Memorial Institute (RPMI-1640) (Mediatech Inc., Manassas, VA) media containing 10% fetal bovine serum (FBS, Life Technologies, Grand Island, NY), 1% Minimum Essential Media (MEM) nonessential amino acids (NEAA, Mediatech) and 1% penicillin–streptomycin (PenStrep, Mediatech). .. HEK-293T cells were newly purchased from ATCC (Manassas, Virginia, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM) (Mediatech) supplemented with 10% FBS, 1% NEAA and 1% PenStrep. .. Normal human renal epithelial cells (RPTC) were purchased from Lifeline Cell Technology (Lifeline Cell Technology, Frederick, MD, USA) and maintained in Lifeline's Renal Life Medium from Lifeline Cell Technology supplemented with 2.4 mM l -glutamine, 5 μg mL–1 rh insulin, 1.0 nM epinephrine, 10 nM triiodothyronine, 0.1 μg mL–1 hydrocortisone hemisuccinate, 10 ng mL–1 rhEGF, 0.50% FBS, 5 μg mL–1 transferrin PS.

Article Title: Bimetallic titanocene-gold phosphane complexes inhibit invasion, metastasis, and angiogenesis-associated signaling molecules in renal cancer
Article Snippet: Human renal cell carcinoma line Caki-1 was newly obtained for these studies from the American Type Culture Collection (ATCC) (Manassas, Virginia, USA) and cultured in Roswell Park Memorial Institute (RPMI-1640) (Mediatech Inc., Manassas, VA) media containing 10% Fetal Bovine Serum, certified, heat inactivated, US origin (FBS) (Gibco, Life Technologies, US), 1% Minimum Essential Media (MEM) nonessential amino acids (NEAA, Mediatech) and 1% penicillin–streptomycin (PenStrep, Mediatech). .. IMR90 (human fetal lung fibroblast) cells were purchased from ATCC (Manassas, Virginia, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM) (Mediatech) supplemented with 10% FBS, 1% NEAA and 1% Penicillin Streptomycin. .. HUVEC (human umbilical vein endothelial) cells were obtained from ATCC and cultured in Medium 200PRF (Gibco, Life Technologies, US).

Article Title: Ionic Silicon Protects Oxidative Damage and Promotes Skeletal Muscle Cell Regeneration
Article Snippet: Materials Sodium metasilicate powder (Na2 SiO3 , MW: 122.06 g/mol, Sigma-Aldrich Co., St. Louis, MO, USA) and hydrogen peroxide (H2 O2 , 30% w /w , Sigma-Aldrich Co., St. Louis, MO, USA) were used as sources of Si-ions and ROS, respectively. .. Dulbecco’s Modified Eagle’s Medium ((DMEM-1×) with 4.5 g/L glucose, L-glutamine, and sodium pyruvate), phosphate-buffered saline (PBS-1×), penicillin-streptomycin (P/S) 10,000 U/mL each, and trypsin EDTA-1× solution were purchased from Mediatech Inc. (Manassas, VA, USA). .. Fetal bovine serum (FBS) and horse serum (HS) were obtained from Thermo Fischer Scientific Inc. (Waltham, MA, USA).

Article Title: Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems
Article Snippet: We performed flow cytometry experiments on either a Beckman-Coulter EPICS XV-MCL flow cytometer or a LSR Fortessa cell analyzer (BD Biosciences) equipped with a 488-nm laser. .. (1) Dulbecco’s modified Eagle medium (DMEM, Mediatech) with 10% heat-deactivated fetal bovine serum (FBS, HyClone) and 1% penicillin–streptomycin (Mediatech); (2) washing media: Dulbecco’s phosphate-buffered saline (DPBS, Invitrogen), DMEM containing no dye, serum, or antibiotics. ..

Article Title: Role for Amino Acids 212KLR214 of Ebola Virus VP40 in Assembly and Budding ▿
Article Snippet: .. Vero and 293T cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (Mediatech) supplemented with 10% fetal calf serum (Invitrogen) and 1× penicillin-streptomycin (Invitrogen) at 5% CO2 at 37°C. .. Plasmid pCAGGS VP40-WT has been described previously ( , ).

Cell Culture:

Article Title: Myxoma Virus M083 Is a Virulence Factor Which Mediates Systemic Dissemination
Article Snippet: .. BSC40 and A549 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum (FBS) and 1× penicillin–streptomycin– l -glutamine (Mediatech, Inc., Manassas, VA, USA). .. RK13 cells were cultured in minimum essential medium (MEM) plus 10% fetal bovine serum and 1× penicillin–streptomycin– l -glutamine (Mediatech, Inc., Manassas, VA, USA).

Article Title: Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus *Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus * SLoss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus * S ◆
Article Snippet: .. After 24 or 48 h of treatment, the cell culture medium was removed, and an equal volume of a 1 mg/ml MTT solution, pH 7.4, in Dulbecco’s modified Eagle’s medium was added to the cells. .. After incubation at 37 °C for 1 h, the MTT solution was removed, and N -propyl alcohol was added to solubilize the formazan product whose absorbance was measured in a spectrophotometer at a wavelength of 560 nm.

MTT Assay:

Article Title: Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus *Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus * SLoss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus * S ◆
Article Snippet: .. After 24 or 48 h of treatment, the cell culture medium was removed, and an equal volume of a 1 mg/ml MTT solution, pH 7.4, in Dulbecco’s modified Eagle’s medium was added to the cells. .. After incubation at 37 °C for 1 h, the MTT solution was removed, and N -propyl alcohol was added to solubilize the formazan product whose absorbance was measured in a spectrophotometer at a wavelength of 560 nm.

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  • 86
    Mediatech dmem f12
    NeuT oncogene transformation enhanced ALA-induced PpIX fluorescence ( A ) Fluorescence spectra of MCF10A vector and NeuT cell lysates and PpIX standard (25 ng/mL in DMSO). Both vector and NeuT cells were incubated with 1 mM ALA in serum free medium for 4 h and lysed. Fluorescence spectra of cell lysates and PpIX standard were obtained using 400 ± 2.5 nm excitation. ( B – D ) Flow cytometer analysis showing forward scatter (FSC) (B), basal cell fluorescence without ALA (C), and a dose-dependent fluorescence increase after ALA incubation (D) in MCF10A vector and NeuT cells. Cells were cultured in serum free <t>DMEM/F12</t> medium with or without ALA for 4 h and cell fluorescence was measured with flow cytometry. ALA-induced fluorescence increase, calculated by subtracting basal cell fluorescence without ALA from cell fluorescence after incubation with different doses of ALA, was fit with the Michaelis-Menten enzyme kinetics. Data are presented as mean ± SD from at least 3 experiments. *** p
    Dmem F12, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem f12/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem f12 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Mediatech serum free dmem f12 medium
    Rapamycin inhibits proliferation and motility in LECs. (A) LECs, grown in serum-free <t>DMEM/F12</t> or the medium supplemented with IGF-1 (10 ng/ml) or FBS (10%), were exposed to rapamycin (0-1000 ng/ml) for 72 hours, followed by cell counting using a Beckman Coulter counter. (B) Cell motility of LECs was determined using the single-cell motility assay. (C) LEC viability was evaluated by one-solution assay. Quantitative data are presented as mean ± SD ( n = 3) in A to C. a P
    Serum Free Dmem F12 Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum free dmem f12 medium/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    serum free dmem f12 medium - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Mediatech dulbecco s modified essential media
    Experimental schema of ex vivo culture of progenitors for human erythroid differentiation. NOD/SCID, nonobese diabetic/severe combined immune deficiency; DMEM, <t>Dulbecco’s</t> modified essential media; FCS, fetal calf serum; BSA, bovine serum albumin;
    Dulbecco S Modified Essential Media, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified essential media/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified essential media - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Mediatech dulbecco s modified eagle s medium dmem
    Endocrine disrupting chemical-induction of adipocyte protein expression. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in <t>Dulbecco’s</t> modified <t>Eagle’s</t> medium <t>(DMEM)</t>
    Dulbecco S Modified Eagle S Medium Dmem, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium dmem/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium dmem - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    NeuT oncogene transformation enhanced ALA-induced PpIX fluorescence ( A ) Fluorescence spectra of MCF10A vector and NeuT cell lysates and PpIX standard (25 ng/mL in DMSO). Both vector and NeuT cells were incubated with 1 mM ALA in serum free medium for 4 h and lysed. Fluorescence spectra of cell lysates and PpIX standard were obtained using 400 ± 2.5 nm excitation. ( B – D ) Flow cytometer analysis showing forward scatter (FSC) (B), basal cell fluorescence without ALA (C), and a dose-dependent fluorescence increase after ALA incubation (D) in MCF10A vector and NeuT cells. Cells were cultured in serum free DMEM/F12 medium with or without ALA for 4 h and cell fluorescence was measured with flow cytometry. ALA-induced fluorescence increase, calculated by subtracting basal cell fluorescence without ALA from cell fluorescence after incubation with different doses of ALA, was fit with the Michaelis-Menten enzyme kinetics. Data are presented as mean ± SD from at least 3 experiments. *** p

    Journal: Oncotarget

    Article Title: Her2 oncogene transformation enhances 5-aminolevulinic acid-mediated protoporphyrin IX production and photodynamic therapy response

    doi: 10.18632/oncotarget.11058

    Figure Lengend Snippet: NeuT oncogene transformation enhanced ALA-induced PpIX fluorescence ( A ) Fluorescence spectra of MCF10A vector and NeuT cell lysates and PpIX standard (25 ng/mL in DMSO). Both vector and NeuT cells were incubated with 1 mM ALA in serum free medium for 4 h and lysed. Fluorescence spectra of cell lysates and PpIX standard were obtained using 400 ± 2.5 nm excitation. ( B – D ) Flow cytometer analysis showing forward scatter (FSC) (B), basal cell fluorescence without ALA (C), and a dose-dependent fluorescence increase after ALA incubation (D) in MCF10A vector and NeuT cells. Cells were cultured in serum free DMEM/F12 medium with or without ALA for 4 h and cell fluorescence was measured with flow cytometry. ALA-induced fluorescence increase, calculated by subtracting basal cell fluorescence without ALA from cell fluorescence after incubation with different doses of ALA, was fit with the Michaelis-Menten enzyme kinetics. Data are presented as mean ± SD from at least 3 experiments. *** p

    Article Snippet: Cell culture and transfection MCF10A human breast epithelial cells were routinely maintained in complete DMEM/F12 (50/50) medium (Mediatech, Manassas, VA) supplemented with 5% horse serum (Atlanta Biologicals), insulin 10 ug/mL, epidermal growth factor (EGF) 20 ng/mL, cholera toxin 100 ng/mL, hydrocortisone 0.5 ug/mL and 1% of antibiotics and antimycotics solution (Mediatech) at 37° C in a humidified 5% CO2 incubator.

    Techniques: Transformation Assay, Fluorescence, Plasmid Preparation, Incubation, Flow Cytometry, Cytometry, Cell Culture

    Her2/NeuT oncogene expression transformed MCF10A human breast epithelial cells ( A ) Differential interference contrast (DIC) images (60×) show distinct differences in cell morphology between MCF10A vector control and NeuT-transformed cells. ( B ) Her2/NeuT oncogene transformation altered cell signaling. MCF10A vector and NeuT cells were cultured in complete DMEM/F12 medium, serum free medium with or without 1 mM ALA for 4 h and lysed with lysis buffer. Cell lysates were examined by Western blot for Her2/Neu signaling molecules, EMT and tight junction markers, and glycolytic enzyme PDK1.

    Journal: Oncotarget

    Article Title: Her2 oncogene transformation enhances 5-aminolevulinic acid-mediated protoporphyrin IX production and photodynamic therapy response

    doi: 10.18632/oncotarget.11058

    Figure Lengend Snippet: Her2/NeuT oncogene expression transformed MCF10A human breast epithelial cells ( A ) Differential interference contrast (DIC) images (60×) show distinct differences in cell morphology between MCF10A vector control and NeuT-transformed cells. ( B ) Her2/NeuT oncogene transformation altered cell signaling. MCF10A vector and NeuT cells were cultured in complete DMEM/F12 medium, serum free medium with or without 1 mM ALA for 4 h and lysed with lysis buffer. Cell lysates were examined by Western blot for Her2/Neu signaling molecules, EMT and tight junction markers, and glycolytic enzyme PDK1.

    Article Snippet: Cell culture and transfection MCF10A human breast epithelial cells were routinely maintained in complete DMEM/F12 (50/50) medium (Mediatech, Manassas, VA) supplemented with 5% horse serum (Atlanta Biologicals), insulin 10 ug/mL, epidermal growth factor (EGF) 20 ng/mL, cholera toxin 100 ng/mL, hydrocortisone 0.5 ug/mL and 1% of antibiotics and antimycotics solution (Mediatech) at 37° C in a humidified 5% CO2 incubator.

    Techniques: Expressing, Transformation Assay, Plasmid Preparation, Cell Culture, Lysis, Western Blot

    Rapamycin inhibits proliferation and motility in LECs. (A) LECs, grown in serum-free DMEM/F12 or the medium supplemented with IGF-1 (10 ng/ml) or FBS (10%), were exposed to rapamycin (0-1000 ng/ml) for 72 hours, followed by cell counting using a Beckman Coulter counter. (B) Cell motility of LECs was determined using the single-cell motility assay. (C) LEC viability was evaluated by one-solution assay. Quantitative data are presented as mean ± SD ( n = 3) in A to C. a P

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Rapamycin Inhibits Lymphatic Endothelial Cell Tube Formation by Downregulating Vascular Endothelial Growth Factor Receptor 3 Protein Expression 1

    doi:

    Figure Lengend Snippet: Rapamycin inhibits proliferation and motility in LECs. (A) LECs, grown in serum-free DMEM/F12 or the medium supplemented with IGF-1 (10 ng/ml) or FBS (10%), were exposed to rapamycin (0-1000 ng/ml) for 72 hours, followed by cell counting using a Beckman Coulter counter. (B) Cell motility of LECs was determined using the single-cell motility assay. (C) LEC viability was evaluated by one-solution assay. Quantitative data are presented as mean ± SD ( n = 3) in A to C. a P

    Article Snippet: For experiments where cells were deprived of serum, cell monolayers were washed with phosphate-buffered saline, and incubated in the serum-free DMEM/F12 medium (Mediatech).

    Techniques: Cell Counting, Motility Assay

    Rapamycin does not alter mRNA expression but inhibits protein synthesis and promotes protein degradation of VEGFR-3. (A) Rapamycin did not affect VEGFR-3 mRNA level. Total RNA was extracted from LECs treated with rapamycin (Rapa, 100 ng/ml) for 24 hours in the presence or absence of IGF-1 (10 ng/ml) or 2% FBS, followed by semiquantitative RT-PCR. β-Actin was used as a loading control. (B) Rapamycin inhibited protein synthesis of VEGFR-3 in LECs. LECs were pretreated with rapamycin (Rapa, 100 ng/ml) for 24 hours, in the presence or absence of IGF-1 (10 ng/ml) or 2% FBS, and then pulsed with 35 S-Met/Cys for 4 hours, followed by immunoprecipitation with antibodies to VEGFR-3. The immunoprecipitates were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes, followed by autoradiography. GAPDH served as an internal control. (C) Rapamycin promoted protein degradation of VEGFR-3 in LECs. LECs, grown in 10% FBS-DMEM/F12 medium, were exposed to CHX (50 µ g/ml), in the presence or absence of rapamycin (Rapa, 100 ng/ml) for 0 to 12 hours, followed by Western blot analysis with the indicated antibodies. Semiquantitative data for A, B, and C by densitometry using ImageJ are shown in D, E, and F, respectively. Results are means ± SD and are pooled from three independent experiments. a P

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Rapamycin Inhibits Lymphatic Endothelial Cell Tube Formation by Downregulating Vascular Endothelial Growth Factor Receptor 3 Protein Expression 1

    doi:

    Figure Lengend Snippet: Rapamycin does not alter mRNA expression but inhibits protein synthesis and promotes protein degradation of VEGFR-3. (A) Rapamycin did not affect VEGFR-3 mRNA level. Total RNA was extracted from LECs treated with rapamycin (Rapa, 100 ng/ml) for 24 hours in the presence or absence of IGF-1 (10 ng/ml) or 2% FBS, followed by semiquantitative RT-PCR. β-Actin was used as a loading control. (B) Rapamycin inhibited protein synthesis of VEGFR-3 in LECs. LECs were pretreated with rapamycin (Rapa, 100 ng/ml) for 24 hours, in the presence or absence of IGF-1 (10 ng/ml) or 2% FBS, and then pulsed with 35 S-Met/Cys for 4 hours, followed by immunoprecipitation with antibodies to VEGFR-3. The immunoprecipitates were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes, followed by autoradiography. GAPDH served as an internal control. (C) Rapamycin promoted protein degradation of VEGFR-3 in LECs. LECs, grown in 10% FBS-DMEM/F12 medium, were exposed to CHX (50 µ g/ml), in the presence or absence of rapamycin (Rapa, 100 ng/ml) for 0 to 12 hours, followed by Western blot analysis with the indicated antibodies. Semiquantitative data for A, B, and C by densitometry using ImageJ are shown in D, E, and F, respectively. Results are means ± SD and are pooled from three independent experiments. a P

    Article Snippet: For experiments where cells were deprived of serum, cell monolayers were washed with phosphate-buffered saline, and incubated in the serum-free DMEM/F12 medium (Mediatech).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, SDS Page, Autoradiography, Western Blot

    Experimental schema of ex vivo culture of progenitors for human erythroid differentiation. NOD/SCID, nonobese diabetic/severe combined immune deficiency; DMEM, Dulbecco’s modified essential media; FCS, fetal calf serum; BSA, bovine serum albumin;

    Journal: Cell transplantation

    Article Title: The Assessment of Human Erythroid Output in NOD/SCID Mice Reconstituted With Human Hematopoietic Stem Cells

    doi: 10.3727/096368910X314161

    Figure Lengend Snippet: Experimental schema of ex vivo culture of progenitors for human erythroid differentiation. NOD/SCID, nonobese diabetic/severe combined immune deficiency; DMEM, Dulbecco’s modified essential media; FCS, fetal calf serum; BSA, bovine serum albumin;

    Article Snippet: In the next 7 days, nonadherent cells were collected and resuspended in 20 ml of media specific for erythroid differentiation from a modified protocol containing Dulbecco’s modified essential media (DMEM, Mediatech, Herdon, VA), 30% FCS, 2% BSA, 10−5 M β-mercaptoethanol, 10−6 M dexamethasone, 0.6 mg/ml human holo-transferrin (Sigma-Aldrich, St. Louis, MO), 2 mM glutamine with penicillin/streptomycin (Invitrogen), 100 ng/ml of SCF, 1.25 ng/ml of transforming growth factor-β (TGF-β, R & D Systems) in the medium, and 5 U/ml rHu erythropoietin (EPO, Amgen), in a 75-cm2 flask at 37°C in 5% CO2 .

    Techniques: Ex Vivo, Modification

    Endocrine disrupting chemical-induction of adipocyte protein expression. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Environmental Endocrine Disruptors Promote Adipogenesis in the 3T3-L1 Cell Line through Glucocorticoid Receptor Activation

    doi: 10.1038/oby.2009.419

    Figure Lengend Snippet: Endocrine disrupting chemical-induction of adipocyte protein expression. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Article Snippet: Two days after reaching confluency, differentiation was initiated by the addition of Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech, Manassas, VA) containing 10% fetal bovine serum (FBS; Aleken Biologicals, Nash, TX), 167 nmol/l porcine insulin, 0–100 nmol/l dehydrocorticosterone (DHC) and 0.5 mmol/l isobutylmethylxanthine (all from Sigma, St Louis, MO).

    Techniques: Expressing, Incubation, Modification

    Dose-dependence of endocrine disrupting chemical-induced adipogenesis. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Environmental Endocrine Disruptors Promote Adipogenesis in the 3T3-L1 Cell Line through Glucocorticoid Receptor Activation

    doi: 10.1038/oby.2009.419

    Figure Lengend Snippet: Dose-dependence of endocrine disrupting chemical-induced adipogenesis. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Article Snippet: Two days after reaching confluency, differentiation was initiated by the addition of Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech, Manassas, VA) containing 10% fetal bovine serum (FBS; Aleken Biologicals, Nash, TX), 167 nmol/l porcine insulin, 0–100 nmol/l dehydrocorticosterone (DHC) and 0.5 mmol/l isobutylmethylxanthine (all from Sigma, St Louis, MO).

    Techniques: Incubation, Modification