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Cellgro dulbecco s modified eagle s medium
Dulbecco S Modified Eagle S Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dulbecco s modified eagle s medium/product/Cellgro
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dulbecco s modified eagle s medium - by Bioz Stars, 2021-03
86/100 stars

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Modification:

Article Title: Serum Amyloid A1 Is an Epithelial Prorestitutive Factor
Article Snippet: .. SAA (1.0 μmol/L)/WRW4 (23.0 μmol/L)/vehicle was added to cells suspended in Dulbecco's modified Eagle's medium before plating. .. Cells were successively immunolabeled with anti–E-cadherin (1:250) and then secondary antibody (anti-rat Alexa Fluor 488 conjugate at 1:1000) to delineate the outer membranes of cells.

Article Title: Development of the Novel PEG-PE-Based Polymer for the Reversible Attachment of Specific Ligands to Liposomes: Synthesis and in vitro Characterization
Article Snippet: Human cervical carcinoma (HeLa) cells and breast cancer cell line (MCF-7) were purchased from ATCC (Manassas, VA). .. Dulbecco’s modified Eagle’s media (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin solution were from CellGro (Kansas City, MO). ..

Article Title: Balance between senescence and apoptosis is regulated by telomere damage–induced association between p16 and caspase-3
Article Snippet: WT, SphK1−/− , and SphK2−/− MEFs were obtained from K. Argraves (Medical University of South Carolina). .. GM00847 cells and MEFs were cultured in Dulbecco's modified Eagle's medium and incubated at 37 °C with 5% CO2 . .. Primary human lung fibroblasts were purchased from Lonza, Inc., and cultured in fibroblast growth media as described ( ).

Article Title: ATR and H2AX Cooperate in Maintaining Genome Stability under Replication Stress
Article Snippet: .. 2 The abbreviations used are: DSB, double-strand break; MEF, murine embryonic fibroblast; 4-OHT, 4-hydroxytamoxifen; HR, homologous recombination; DMEM, Dulbecco's modified Eagle's medium; shRNA, short hairpin RNA; FBS, fetal bovine serum; BrdUrd, bromodeoxyuridine; shATR, shRNA targeting ATR; shCtrl, shRNA targeting control; DNA-PKcs, catalytic subunit of DNA-dependent protein kinase. ..

Article Title: G Protein-coupled Receptor Kinase-2 Constitutively Regulates D2 Dopamine Receptor Expression and Signaling Independently of Receptor Phosphorylation *
Article Snippet: [3 H]Sulpiride (70–87 Ci/mmol), [3 H]methylspiperone (80–85 Ci/mmol), [32 P]orthophosphate (carrier-free), and [3 H]cAMP (25–40 Ci/mmol) were purchased from PerkinElmer Life Sciences. .. Dulbecco's modified Eagle's medium (DMEM) was from Cellgro® Mediatech, Inc. (Herndon, VA). .. Fetal calf serum and other cell culture reagents were purchased from Invitrogen.

Article Title: miR-1289 and "Zipcode"-like Sequence Enrich mRNAs in Microvesicles
Article Snippet: Cultures of HEK-293T cells (75–80% confluent) were transfected with 7 µg pEGFP-N1 original or derived plasmids per each 200-mm plate with Lipofectamine 2000 (Invitrogen, Carlsbad, CA; 11668-019), according to manufacturer's protocol. .. Six hours later, transfection media was removed and replaced with Dulbecco's modified Eagle's medium containing 5% MV-free fetal bovine serum. .. MV-free fetal bovine serum is obtained after the ultracentrifugation and filtration process.

Article Title:
Article Snippet: .. At 80–90% confluency (the following morning), 0.5 ml of warm incubation media composed of Dulbecco’s modified Eagle’s medium with 0.9 g/l NaCl, 2.5 μ Ci/ml [3 H]adenine and 0.5 mM IBMX was added to the cells. .. After a 4-hour incubation period at 37°C in a 5% CO2 incubator, the media was removed and the plate was briefly floated on an ice water bath while 0.5 ml of an assay mix was quickly added to the cells in triplicate.

Cell Culture:

Article Title: Balance between senescence and apoptosis is regulated by telomere damage–induced association between p16 and caspase-3
Article Snippet: WT, SphK1−/− , and SphK2−/− MEFs were obtained from K. Argraves (Medical University of South Carolina). .. GM00847 cells and MEFs were cultured in Dulbecco's modified Eagle's medium and incubated at 37 °C with 5% CO2 . .. Primary human lung fibroblasts were purchased from Lonza, Inc., and cultured in fibroblast growth media as described ( ).

Incubation:

Article Title: Balance between senescence and apoptosis is regulated by telomere damage–induced association between p16 and caspase-3
Article Snippet: WT, SphK1−/− , and SphK2−/− MEFs were obtained from K. Argraves (Medical University of South Carolina). .. GM00847 cells and MEFs were cultured in Dulbecco's modified Eagle's medium and incubated at 37 °C with 5% CO2 . .. Primary human lung fibroblasts were purchased from Lonza, Inc., and cultured in fibroblast growth media as described ( ).

Article Title: Brucella abortus Strain RB51 Leucine Auxotroph as an Environmentally Safe Vaccine for Plasmid Maintenance and Antigen Overexpression ▿ Strain RB51 Leucine Auxotroph as an Environmentally Safe Vaccine for Plasmid Maintenance and Antigen Overexpression ▿ †
Article Snippet: A 48-h culture of the pNS4/GFP-complemented strain RB51 leuB was resuspended in phosphate-buffered saline and used to infect the macrophages at a 100:1 (bacteria/macrophage) multiplicity of infection. .. After 45 min, the macrophages were washed three times with phosphate-buffered saline and then incubated in Dulbecco's modified Eagle's medium containing 100 μg/ml of streptomycin-penicillin. ..

Article Title:
Article Snippet: .. At 80–90% confluency (the following morning), 0.5 ml of warm incubation media composed of Dulbecco’s modified Eagle’s medium with 0.9 g/l NaCl, 2.5 μ Ci/ml [3 H]adenine and 0.5 mM IBMX was added to the cells. .. After a 4-hour incubation period at 37°C in a 5% CO2 incubator, the media was removed and the plate was briefly floated on an ice water bath while 0.5 ml of an assay mix was quickly added to the cells in triplicate.

Homologous Recombination:

Article Title: ATR and H2AX Cooperate in Maintaining Genome Stability under Replication Stress
Article Snippet: .. 2 The abbreviations used are: DSB, double-strand break; MEF, murine embryonic fibroblast; 4-OHT, 4-hydroxytamoxifen; HR, homologous recombination; DMEM, Dulbecco's modified Eagle's medium; shRNA, short hairpin RNA; FBS, fetal bovine serum; BrdUrd, bromodeoxyuridine; shATR, shRNA targeting ATR; shCtrl, shRNA targeting control; DNA-PKcs, catalytic subunit of DNA-dependent protein kinase. ..

shRNA:

Article Title: ATR and H2AX Cooperate in Maintaining Genome Stability under Replication Stress
Article Snippet: .. 2 The abbreviations used are: DSB, double-strand break; MEF, murine embryonic fibroblast; 4-OHT, 4-hydroxytamoxifen; HR, homologous recombination; DMEM, Dulbecco's modified Eagle's medium; shRNA, short hairpin RNA; FBS, fetal bovine serum; BrdUrd, bromodeoxyuridine; shATR, shRNA targeting ATR; shCtrl, shRNA targeting control; DNA-PKcs, catalytic subunit of DNA-dependent protein kinase. ..

Transfection:

Article Title: miR-1289 and "Zipcode"-like Sequence Enrich mRNAs in Microvesicles
Article Snippet: Cultures of HEK-293T cells (75–80% confluent) were transfected with 7 µg pEGFP-N1 original or derived plasmids per each 200-mm plate with Lipofectamine 2000 (Invitrogen, Carlsbad, CA; 11668-019), according to manufacturer's protocol. .. Six hours later, transfection media was removed and replaced with Dulbecco's modified Eagle's medium containing 5% MV-free fetal bovine serum. .. MV-free fetal bovine serum is obtained after the ultracentrifugation and filtration process.

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  • 86
    Cellgro dmem f12
    Anti-fibrotic effects of Rosiglitazone on cultured feline corneal fibroblast. A . Representative western blots showing protein levels for αSMA. Tubulin levels were assayed as a loading control. For this experiment, cells were pretreated with 25 µM, 50 µM, and 75 µM Rosiglitazone for 30 min, before adding 1 ng/ml of TGFβ in <t>DMEM/F12</t> containing 1% HS. The cells were cultured in this treated medium for 1, 2 or 3 days and then harvested for western blotting. While some effect could be observed at lower doses, Rosiglitazone clearly inhibited αSMA expression at 75 µM, while tubulin levels remained stable. B . Plots of relative expression of αSMA normalized to densitometric values obtained in cells stimulated with 1 ng/ml TGFβ for each culture day sampled. Data shown are means±SD, averaged over 3 experiments, and they confirm a statistically significant inhibitory effect of Rosiglitazone on αSMA. * P
    Dmem F12, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem f12/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem f12 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cellgro dmem
    Cell-survival quantification with the XTT assay after treatment with recombinant NGF (open bars) or proNGF123 (filled bars) at the concentrations indicated for 72 h in defined <t>DMEM.</t> RN22 <t>schwannoma</t> cells ( A ), PC12nnr cells ( B ), and C6 glioma cells ( C
    Dmem, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cellgro dmem medium
    Cytokine production by liver immune cells and adipose tissue (AT) stromal vascular cells <t>(SVC).</t> Isolated immune cells from liver (upper panel) and SVC from AT (lower panel) were cultured for 18 h in complete <t>DMEM</t> medium. The harvested media was analyzed for the indicated cytokines using Luminex. Data are presented as mean ± SE (minimum of five animals/group analyzed individually). Significant differences are indicated (* P
    Dmem Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem medium/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem medium - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Anti-fibrotic effects of Rosiglitazone on cultured feline corneal fibroblast. A . Representative western blots showing protein levels for αSMA. Tubulin levels were assayed as a loading control. For this experiment, cells were pretreated with 25 µM, 50 µM, and 75 µM Rosiglitazone for 30 min, before adding 1 ng/ml of TGFβ in DMEM/F12 containing 1% HS. The cells were cultured in this treated medium for 1, 2 or 3 days and then harvested for western blotting. While some effect could be observed at lower doses, Rosiglitazone clearly inhibited αSMA expression at 75 µM, while tubulin levels remained stable. B . Plots of relative expression of αSMA normalized to densitometric values obtained in cells stimulated with 1 ng/ml TGFβ for each culture day sampled. Data shown are means±SD, averaged over 3 experiments, and they confirm a statistically significant inhibitory effect of Rosiglitazone on αSMA. * P

    Journal: PLoS ONE

    Article Title: Topical Rosiglitazone Is an Effective Anti-Scarring Agent in the Cornea

    doi: 10.1371/journal.pone.0070785

    Figure Lengend Snippet: Anti-fibrotic effects of Rosiglitazone on cultured feline corneal fibroblast. A . Representative western blots showing protein levels for αSMA. Tubulin levels were assayed as a loading control. For this experiment, cells were pretreated with 25 µM, 50 µM, and 75 µM Rosiglitazone for 30 min, before adding 1 ng/ml of TGFβ in DMEM/F12 containing 1% HS. The cells were cultured in this treated medium for 1, 2 or 3 days and then harvested for western blotting. While some effect could be observed at lower doses, Rosiglitazone clearly inhibited αSMA expression at 75 µM, while tubulin levels remained stable. B . Plots of relative expression of αSMA normalized to densitometric values obtained in cells stimulated with 1 ng/ml TGFβ for each culture day sampled. Data shown are means±SD, averaged over 3 experiments, and they confirm a statistically significant inhibitory effect of Rosiglitazone on αSMA. * P

    Article Snippet: After attachment, which usually took 1–1.5 hrs, the medium was changed to one containing DMEM/F12 with 1% HS for 1day in order to promote cellular quiescence.

    Techniques: Cell Culture, Western Blot, Expressing

    Cell-survival quantification with the XTT assay after treatment with recombinant NGF (open bars) or proNGF123 (filled bars) at the concentrations indicated for 72 h in defined DMEM. RN22 schwannoma cells ( A ), PC12nnr cells ( B ), and C6 glioma cells ( C

    Journal:

    Article Title: Construction of a mutated pro-nerve growth factor resistant to degradation and suitable for biophysical and cellular utilization

    doi: 10.1073/pnas.0604139103

    Figure Lengend Snippet: Cell-survival quantification with the XTT assay after treatment with recombinant NGF (open bars) or proNGF123 (filled bars) at the concentrations indicated for 72 h in defined DMEM. RN22 schwannoma cells ( A ), PC12nnr cells ( B ), and C6 glioma cells ( C

    Article Snippet: PC12 (from Lloyd A. Greene, Columbia University, New York, NY), PC12nnr (from Phil Barker, McGill University, Montreal, CA), C6 glioma (from ATCC, Manassas, VA), and RN22 schwannoma (from Bruce Carter, Vanderbilt University, Nashville, TN) cells were grown in DMEM (Cellgro; Voigt Global Distribution, Kansas City, MO) supplemented with 10% horse serum, 5% FBS, 4.5 mg/ml glucose, 4.0 mM l -glutamine, 100 units/ml penicillin, 100 pg/ml streptomycin, and 0.25 pg/ml amphotericin-B25 at 37°C in a humid atmosphere containing 5% CO2 .

    Techniques: XTT Assay, Recombinant

    Relationship between the incidence of PC and rate of proliferation in 184A1 and MDA-MB-231. ( A,B ) 184A1 was cultured in three different media: DMEM without FBS (D S−), DMEM with 10% FBS (D S+), and optimal basal medium with supplements and growth factors (MEM). PC were labeled by anti-acetylated α-tubulin (red) and proliferating cells by anti-Ki-67 (green). ( A ) PC were located only in non-proliferating Ki-67-negative cells. ( B ) The incidence of PC (red bars) in 184A1 cells was higher in serum-free conditions (D S−) and with longer duration of culture. Data are the mean and standard error of two independent experiments performed in triplicate. ( C ) MDA-MB-231 were grown in DMEM with (D S+) and without (D S−) serum. Proliferation (Ki-67 labeling, green bars) was lower in serum-free conditions; however, there was no corresponding statistically significant increase in PC (red bars). Data are the mean of two independent experiments performed in triplicate. Bar: A = 10 μm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Primary Cilia Are Decreased in Breast Cancer: Analysis of a Collection of Human Breast Cancer Cell Lines and Tissues

    doi: 10.1369/jhc.2010.955856

    Figure Lengend Snippet: Relationship between the incidence of PC and rate of proliferation in 184A1 and MDA-MB-231. ( A,B ) 184A1 was cultured in three different media: DMEM without FBS (D S−), DMEM with 10% FBS (D S+), and optimal basal medium with supplements and growth factors (MEM). PC were labeled by anti-acetylated α-tubulin (red) and proliferating cells by anti-Ki-67 (green). ( A ) PC were located only in non-proliferating Ki-67-negative cells. ( B ) The incidence of PC (red bars) in 184A1 cells was higher in serum-free conditions (D S−) and with longer duration of culture. Data are the mean and standard error of two independent experiments performed in triplicate. ( C ) MDA-MB-231 were grown in DMEM with (D S+) and without (D S−) serum. Proliferation (Ki-67 labeling, green bars) was lower in serum-free conditions; however, there was no corresponding statistically significant increase in PC (red bars). Data are the mean of two independent experiments performed in triplicate. Bar: A = 10 μm.

    Article Snippet: MDA-MB-231, MDA-MB-361, MDA-MB-468, Hs578T, MDA-MB-435, MDA435/LCC6, MCF10DCIS.com, MCF10CA1, and MCF7 cells were maintained in DMEM (Cellgro; Manassas, VA) supplemented with 10% FBS (Hyclone; Logan, UT).

    Techniques: Multiple Displacement Amplification, Cell Culture, Labeling

    Cytokine production by liver immune cells and adipose tissue (AT) stromal vascular cells (SVC). Isolated immune cells from liver (upper panel) and SVC from AT (lower panel) were cultured for 18 h in complete DMEM medium. The harvested media was analyzed for the indicated cytokines using Luminex. Data are presented as mean ± SE (minimum of five animals/group analyzed individually). Significant differences are indicated (* P

    Journal: Physiological Reports

    Article Title: Similar degrees of obesity induced by diet or aging cause strikingly different immunologic and metabolic outcomes. Similar degrees of obesity induced by diet or aging cause strikingly different immunologic and metabolic outcomes

    doi: 10.14814/phy2.12708

    Figure Lengend Snippet: Cytokine production by liver immune cells and adipose tissue (AT) stromal vascular cells (SVC). Isolated immune cells from liver (upper panel) and SVC from AT (lower panel) were cultured for 18 h in complete DMEM medium. The harvested media was analyzed for the indicated cytokines using Luminex. Data are presented as mean ± SE (minimum of five animals/group analyzed individually). Significant differences are indicated (* P

    Article Snippet: Tissue and plasma measurements The isolated immune cells from liver and SVC from AT were cultured for 18 h in complete DMEM medium (Cellgro, Manassas, VA).

    Techniques: Isolation, Cell Culture, Luminex