dulbecco s modified eagle s medium  (ATCC)


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    Name:
    Dulbecco s Modified Eagle s Medium DMEM
    Description:
    Dulbecco s Modified Eagle s Medium DMEM modified to contain 4 mM L glutamine 4500 mg L glucose 1 mM sodium pyruvate and 1500 mg L sodium bicarbonate
    Catalog Number:
    30-2002
    Price:
    None
    Applications:
    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.
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    Structured Review

    ATCC dulbecco s modified eagle s medium
    Dulbecco s Modified Eagle s Medium DMEM modified to contain 4 mM L glutamine 4500 mg L glucose 1 mM sodium pyruvate and 1500 mg L sodium bicarbonate
    https://www.bioz.com/result/dulbecco s modified eagle s medium/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Adipose-derived mesenchymal stem cells promote the malignant phenotype of cervical cancer
    Article Snippet: Briefly, freshly tissues were washed with PBS and minced into small pieces and samples were incubated with collagenase I at 0.075% (SCR103, Millipore USA MA) for 40 min at 37 °C with gently shaker. .. After centrifugation, the stromal fraction containing the ADSC was collected and seeded with DMEM medium supplemented with streptomycin/penicillin 1X (30-2300 ATCC, Virginia, USA) and 5% FBS. ..

    Modification:

    Article Title: The First Scale-Up Production of Theranostic Nanoemulsions
    Article Snippet: Cremophor EL (CrEL) was purchased from Sigma Aldrich. .. Miglyol 812N, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and the Raw 264.7 cell line were purchased from ATCC. .. Prostaglandin E2 EIA Kit-monoclonal was purchased from Cayman Chemical Company.

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

    Article Title: Subcellular Distribution of S-Nitrosylated H-Ras in Differentiated and Undifferentiated PC12 Cells during Hypoxia
    Article Snippet: .. PC12 cell line In our experimental study, we cultured PC12 (Adh; ATCC® CRL1721.1™) cells in T25 flasks (Greiner Bio-One GmbH, Cat. No.: 690 170) in a humidified atmosphere that contained 5% CO 2 at 37˚C in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC® 30-2002™) supplemented with 10% heat- inactivated horse serum (Sigma-Aldrich), 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 IU/ml penicillin and 50 µg/ml gentamycin sulphate. .. For the induction of differentiation, PC12 cells were incubated in low serum-DMEM that consisted of 1% heat-inactivated horse serum and 1% FBS, supplemented with nerve growth factor (NGF) 100 ng/ml for 5 days.

    Article Title: A Cost-Effective Approach for Non-Persistent Gold Nano-Architectures Production
    Article Snippet: Cell Culture MIA PaCa-2, SCC-25, and UPCI:SCC154 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). .. SCC-25 (ATCC® CRL-1628™) was maintained in a complete Dulbecco’s modified Eagle medium (DMEM)/F12 medium (1:1), while UPCI:SCC154 (ATCC® CRL-3241™) and MIA PaCa-2 (ATCC® CRM-CRL-1420™) were grown in DMEM from Invitrogen (Carlsbad, CA). .. Both growth mediums were supplemented with 10% fetal bovine serum (FBS), 4 mM L-glutamine, 1 mM sodium pyruvate, 100 U/mL penicillin, and 100 mg/mL streptomycin (Invitrogen).

    Derivative Assay:

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

    Cell Culture:

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

    Article Title: Subcellular Distribution of S-Nitrosylated H-Ras in Differentiated and Undifferentiated PC12 Cells during Hypoxia
    Article Snippet: .. PC12 cell line In our experimental study, we cultured PC12 (Adh; ATCC® CRL1721.1™) cells in T25 flasks (Greiner Bio-One GmbH, Cat. No.: 690 170) in a humidified atmosphere that contained 5% CO 2 at 37˚C in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC® 30-2002™) supplemented with 10% heat- inactivated horse serum (Sigma-Aldrich), 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 IU/ml penicillin and 50 µg/ml gentamycin sulphate. .. For the induction of differentiation, PC12 cells were incubated in low serum-DMEM that consisted of 1% heat-inactivated horse serum and 1% FBS, supplemented with nerve growth factor (NGF) 100 ng/ml for 5 days.

    Article Title: Adipose-derived mesenchymal stem cells promote the malignant phenotype of cervical cancer
    Article Snippet: Our results showed that ADSCs promote cell movement, angiogenesis, migration, and the epithelial–mesenchymal transition (EMT) and increase the malignant properties of CC cells through the positive regulation of NF-kappa B signaling, a pathway involved in initiation, progression and resistance to treatment in various types of cancer. .. Cell culture HeLa, SiHa, CaSki, HaCaT and ADSC were obtained from ATCC (Manassas, VA, USA) and cultured in DMEM medium supplied with 5% fetal bovine serum (FBS) (ATCC, 30-2020) at 37 °C/5% CO2 . .. To grow and expand ADSCs we used a commercially available medium (Mesenchymal Stem Cell Basal Medium, ATCC PCS 500030) containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts.

    Isolation:

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

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  • ls174t  (ATCC)
    99
    ATCC ls174t
    EC958 adhesion and invasion of mucus-producing <t>LS174T</t> human colorectal epithelial cells and human intestinal biopsy specimens. A , LS174T monolayers were incubated with EC958 wild-type (WT) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria). Micrographs depict immunofluorescence staining of monolayers (n = 5 in duplicate); MUC2 is stained green, and red is stained EC958 WT. Scale bar, 20 µm. B and C , Immunofluorescence staining of human colonic ( B ) and ileal ( C ) biopsy specimens infected with EC958 WT for 7 hours with corresponding non-infected controls (n = 2 in duplicate). Tissue specimens were stained for actin (green) and EC958 (red). Scale bar, 50 µm. D , Human ileal biopsy specimens infected with EC958 for 8 hours (n = 2 in duplicate). Adherent EC958 (red) are present on exposed submucosal tissue (villus on right, indicated with white arrows) but not on intact epithelium (villus on left), which is indicated by an actin-rich (green) brush border. Cell nuclei are counterstained in blue.
    Ls174t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ls174t/product/ATCC
    Average 99 stars, based on 1 article reviews
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    93
    ATCC mda mb 134 vi
    IGF1-IGF1R pathway is active in invasive lobular breast carcinoma with genetic loss of CDH1 . (A) SUM44PE, (B) <t>MDA-MB-134,</t> and (C) BCK4 ILC cells were immunostained for IGF1R (green) and E-cadherin (red) and imaged by confocal microscopy. Of note, BCK4 cells were imaged at an increased exposure compared to MM134 and SUM44PE cells. (D) CDH1 mRNA, (E) IGF1 mRNA, (F) IGF2 mRNA, and (G) pIGF1R/InsR levels in ER+ IDC compared to ER+ ILC in TCGA were plotted using RNAseq (log2 TPM+1) and RPPA (median normalized) data. The TCGA cohort includes n=417 IDC cases and n=137 ILC cases that have matched data for RNAseq and RPPA. Man-Whitney test was used to determine significant differences in expression level between the two subtypes, p
    Mda Mb 134 Vi, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    t84  (ATCC)
    97
    ATCC t84
    Adhesion to and invasion of <t>T84</t> and Caco-2 human intestinal epithelial cells by uropathogenic Escherichia coli (UPEC) and commensal E. coli strains. T84 ( A – C ) and Caco-2 ( D – F ) monolayers were incubated with UPEC (strains EC958, UTI89, and CFT073), probiotic E. coli Nissle 1917, or commensal E. coli (strains F-18 and MG1655) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin (to determine the number of CFUs of intracellular bacteria). Invasion frequencies are expressed as percentages of adherent bacteria invading the cells. Box plots summarize data from at least 4 experimental repeats. * P
    T84, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
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    Image Search Results


    EC958 adhesion and invasion of mucus-producing LS174T human colorectal epithelial cells and human intestinal biopsy specimens. A , LS174T monolayers were incubated with EC958 wild-type (WT) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria). Micrographs depict immunofluorescence staining of monolayers (n = 5 in duplicate); MUC2 is stained green, and red is stained EC958 WT. Scale bar, 20 µm. B and C , Immunofluorescence staining of human colonic ( B ) and ileal ( C ) biopsy specimens infected with EC958 WT for 7 hours with corresponding non-infected controls (n = 2 in duplicate). Tissue specimens were stained for actin (green) and EC958 (red). Scale bar, 50 µm. D , Human ileal biopsy specimens infected with EC958 for 8 hours (n = 2 in duplicate). Adherent EC958 (red) are present on exposed submucosal tissue (villus on right, indicated with white arrows) but not on intact epithelium (villus on left), which is indicated by an actin-rich (green) brush border. Cell nuclei are counterstained in blue.

    Journal: The Journal of Infectious Diseases

    Article Title: Intestinal Colonization Traits of Pandemic Multidrug-Resistant Escherichia coli ST131

    doi: 10.1093/infdis/jiy031

    Figure Lengend Snippet: EC958 adhesion and invasion of mucus-producing LS174T human colorectal epithelial cells and human intestinal biopsy specimens. A , LS174T monolayers were incubated with EC958 wild-type (WT) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria). Micrographs depict immunofluorescence staining of monolayers (n = 5 in duplicate); MUC2 is stained green, and red is stained EC958 WT. Scale bar, 20 µm. B and C , Immunofluorescence staining of human colonic ( B ) and ileal ( C ) biopsy specimens infected with EC958 WT for 7 hours with corresponding non-infected controls (n = 2 in duplicate). Tissue specimens were stained for actin (green) and EC958 (red). Scale bar, 50 µm. D , Human ileal biopsy specimens infected with EC958 for 8 hours (n = 2 in duplicate). Adherent EC958 (red) are present on exposed submucosal tissue (villus on right, indicated with white arrows) but not on intact epithelium (villus on left), which is indicated by an actin-rich (green) brush border. Cell nuclei are counterstained in blue.

    Article Snippet: Epithelial Cell Adhesion and Invasion Assays Intestinal epithelial cells Caco-2 (ATCC HTB-37; in Dulbecco’s modified Eagle’s medium [DMEM]), T84 (ATCC CCL-248; in DMEM and Ham’s F-12 nutrient mixture), and LS174T (ATCC CL-188; in DMEM) were maintained in medium (Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen).

    Techniques: Incubation, Immunofluorescence, Staining, Infection

    IGF1-IGF1R pathway is active in invasive lobular breast carcinoma with genetic loss of CDH1 . (A) SUM44PE, (B) MDA-MB-134, and (C) BCK4 ILC cells were immunostained for IGF1R (green) and E-cadherin (red) and imaged by confocal microscopy. Of note, BCK4 cells were imaged at an increased exposure compared to MM134 and SUM44PE cells. (D) CDH1 mRNA, (E) IGF1 mRNA, (F) IGF2 mRNA, and (G) pIGF1R/InsR levels in ER+ IDC compared to ER+ ILC in TCGA were plotted using RNAseq (log2 TPM+1) and RPPA (median normalized) data. The TCGA cohort includes n=417 IDC cases and n=137 ILC cases that have matched data for RNAseq and RPPA. Man-Whitney test was used to determine significant differences in expression level between the two subtypes, p

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Loss of E-cadherin enhances IGF1-IGF1R pathway activation and sensitizes breast cancers to anti-IGF1R/InsR inhibitors

    doi: 10.1158/1078-0432.CCR-18-0279

    Figure Lengend Snippet: IGF1-IGF1R pathway is active in invasive lobular breast carcinoma with genetic loss of CDH1 . (A) SUM44PE, (B) MDA-MB-134, and (C) BCK4 ILC cells were immunostained for IGF1R (green) and E-cadherin (red) and imaged by confocal microscopy. Of note, BCK4 cells were imaged at an increased exposure compared to MM134 and SUM44PE cells. (D) CDH1 mRNA, (E) IGF1 mRNA, (F) IGF2 mRNA, and (G) pIGF1R/InsR levels in ER+ IDC compared to ER+ ILC in TCGA were plotted using RNAseq (log2 TPM+1) and RPPA (median normalized) data. The TCGA cohort includes n=417 IDC cases and n=137 ILC cases that have matched data for RNAseq and RPPA. Man-Whitney test was used to determine significant differences in expression level between the two subtypes, p

    Article Snippet: MCF-7 (ATCC; DMEM+10% FBS [06/29/16]), T47D (ATCC; RPMI+10% FBS [02/08/17]), ZR75.1 (ATCC; RPMI+10% FBS [10/13/16]), MDA-MB-231 (ATCC; DMEM+10% FBS [10/13/16]), MDA-MB-134-VI (ATCC; 50/50 DMEM/L15+10% FBS [02/08/17]), SUM44PE (Asterand; DMEM/F12+2% CSS with 5ug/ml insulin, 1ug/ml hydrocortisone, 5mM ethanolamine, 5ug/ml transferrin, 10nM triodothyronime, and 50nM sodium selenite [02/08/17 – no reference profile exists in database]), and BCK4 (MEM+5% FBS with 1nM insulin and 1× NEAA [10/13/16 – no reference profile exists in database) cells were cultured with indicated media conditions.

    Techniques: Multiple Displacement Amplification, Confocal Microscopy, Expressing

    Adhesion to and invasion of T84 and Caco-2 human intestinal epithelial cells by uropathogenic Escherichia coli (UPEC) and commensal E. coli strains. T84 ( A – C ) and Caco-2 ( D – F ) monolayers were incubated with UPEC (strains EC958, UTI89, and CFT073), probiotic E. coli Nissle 1917, or commensal E. coli (strains F-18 and MG1655) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin (to determine the number of CFUs of intracellular bacteria). Invasion frequencies are expressed as percentages of adherent bacteria invading the cells. Box plots summarize data from at least 4 experimental repeats. * P

    Journal: The Journal of Infectious Diseases

    Article Title: Intestinal Colonization Traits of Pandemic Multidrug-Resistant Escherichia coli ST131

    doi: 10.1093/infdis/jiy031

    Figure Lengend Snippet: Adhesion to and invasion of T84 and Caco-2 human intestinal epithelial cells by uropathogenic Escherichia coli (UPEC) and commensal E. coli strains. T84 ( A – C ) and Caco-2 ( D – F ) monolayers were incubated with UPEC (strains EC958, UTI89, and CFT073), probiotic E. coli Nissle 1917, or commensal E. coli (strains F-18 and MG1655) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin (to determine the number of CFUs of intracellular bacteria). Invasion frequencies are expressed as percentages of adherent bacteria invading the cells. Box plots summarize data from at least 4 experimental repeats. * P

    Article Snippet: Epithelial Cell Adhesion and Invasion Assays Intestinal epithelial cells Caco-2 (ATCC HTB-37; in Dulbecco’s modified Eagle’s medium [DMEM]), T84 (ATCC CCL-248; in DMEM and Ham’s F-12 nutrient mixture), and LS174T (ATCC CL-188; in DMEM) were maintained in medium (Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen).

    Techniques: Incubation

    Role of type 1 fimbriae in EC958 adhesion and invasion of T84 and Caco-2 cells. T84 ( A – C ) and Caco-2 ( D – F ) monolayers were incubated with the EC958 wild-type (WT) strain, the type 1 fimbriae null mutant (EC958Δ fimH ), or its complemented derivative (EC958 fimH C ) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria; A and D ) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria; B and E ). Invasion frequencies ( C and F ) are expressed as percentages of adherent bacteria invading the cells. Box plots summarize data from at least 4 experimental repeats. * P

    Journal: The Journal of Infectious Diseases

    Article Title: Intestinal Colonization Traits of Pandemic Multidrug-Resistant Escherichia coli ST131

    doi: 10.1093/infdis/jiy031

    Figure Lengend Snippet: Role of type 1 fimbriae in EC958 adhesion and invasion of T84 and Caco-2 cells. T84 ( A – C ) and Caco-2 ( D – F ) monolayers were incubated with the EC958 wild-type (WT) strain, the type 1 fimbriae null mutant (EC958Δ fimH ), or its complemented derivative (EC958 fimH C ) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria; A and D ) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria; B and E ). Invasion frequencies ( C and F ) are expressed as percentages of adherent bacteria invading the cells. Box plots summarize data from at least 4 experimental repeats. * P

    Article Snippet: Epithelial Cell Adhesion and Invasion Assays Intestinal epithelial cells Caco-2 (ATCC HTB-37; in Dulbecco’s modified Eagle’s medium [DMEM]), T84 (ATCC CCL-248; in DMEM and Ham’s F-12 nutrient mixture), and LS174T (ATCC CL-188; in DMEM) were maintained in medium (Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen).

    Techniques: Incubation, Mutagenesis

    Adhesion to and invasion of T84 and Caco-2 human intestinal epithelial cells by different ST131 strains. T84 ( A – C ) and Caco-2 ( D – F ) monolayers were incubated with ST131 strains from clades A, B, and C for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria; A and D ) and then treated with gentamicin \for 1 hour (to determine the number of CFUs of intracellular bacteria; B and E ). Invasion frequencies are expressed as percentages of adherent bacteria invading the cells ( C and F ). Box plots summarize data from at least 4 experimental repeats. * P

    Journal: The Journal of Infectious Diseases

    Article Title: Intestinal Colonization Traits of Pandemic Multidrug-Resistant Escherichia coli ST131

    doi: 10.1093/infdis/jiy031

    Figure Lengend Snippet: Adhesion to and invasion of T84 and Caco-2 human intestinal epithelial cells by different ST131 strains. T84 ( A – C ) and Caco-2 ( D – F ) monolayers were incubated with ST131 strains from clades A, B, and C for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria; A and D ) and then treated with gentamicin \for 1 hour (to determine the number of CFUs of intracellular bacteria; B and E ). Invasion frequencies are expressed as percentages of adherent bacteria invading the cells ( C and F ). Box plots summarize data from at least 4 experimental repeats. * P

    Article Snippet: Epithelial Cell Adhesion and Invasion Assays Intestinal epithelial cells Caco-2 (ATCC HTB-37; in Dulbecco’s modified Eagle’s medium [DMEM]), T84 (ATCC CCL-248; in DMEM and Ham’s F-12 nutrient mixture), and LS174T (ATCC CL-188; in DMEM) were maintained in medium (Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen).

    Techniques: Incubation