dulbecco s modified eagle s medium dmem  (atcc)


Bioz Verified Symbol atcc is a verified supplier
Bioz Manufacturer Symbol atcc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    Dulbecco s Modified Eagle s Medium DMEM
    Description:
    Dulbecco s Modified Eagle s Medium DMEM modified to contain 4 mM L glutamine 4500 mg L glucose 1 mM sodium pyruvate and 1500 mg L sodium bicarbonate
    Catalog Number:
    30-2002
    Price:
    None
    Applications:
    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.
    Buy from Supplier


    Structured Review

    atcc dulbecco s modified eagle s medium dmem
    Dulbecco s Modified Eagle s Medium DMEM modified to contain 4 mM L glutamine 4500 mg L glucose 1 mM sodium pyruvate and 1500 mg L sodium bicarbonate
    https://www.bioz.com/result/dulbecco s modified eagle s medium dmem/product/atcc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium dmem - by Bioz Stars, 2021-03
    86/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Adipose-derived mesenchymal stem cells promote the malignant phenotype of cervical cancer
    Article Snippet: Briefly, freshly tissues were washed with PBS and minced into small pieces and samples were incubated with collagenase I at 0.075% (SCR103, Millipore USA MA) for 40 min at 37 °C with gently shaker. .. After centrifugation, the stromal fraction containing the ADSC was collected and seeded with DMEM medium supplemented with streptomycin/penicillin 1X (30-2300 ATCC, Virginia, USA) and 5% FBS. ..

    Modification:

    Article Title: The First Scale-Up Production of Theranostic Nanoemulsions
    Article Snippet: Cremophor EL (CrEL) was purchased from Sigma Aldrich. .. Miglyol 812N, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and the Raw 264.7 cell line were purchased from ATCC. .. Prostaglandin E2 EIA Kit-monoclonal was purchased from Cayman Chemical Company.

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

    Article Title: Subcellular Distribution of S-Nitrosylated H-Ras in Differentiated and Undifferentiated PC12 Cells during Hypoxia
    Article Snippet: .. PC12 cell line In our experimental study, we cultured PC12 (Adh; ATCC® CRL1721.1™) cells in T25 flasks (Greiner Bio-One GmbH, Cat. No.: 690 170) in a humidified atmosphere that contained 5% CO 2 at 37˚C in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC® 30-2002™) supplemented with 10% heat- inactivated horse serum (Sigma-Aldrich), 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 IU/ml penicillin and 50 µg/ml gentamycin sulphate. .. For the induction of differentiation, PC12 cells were incubated in low serum-DMEM that consisted of 1% heat-inactivated horse serum and 1% FBS, supplemented with nerve growth factor (NGF) 100 ng/ml for 5 days.

    Article Title: A Cost-Effective Approach for Non-Persistent Gold Nano-Architectures Production
    Article Snippet: Cell Culture MIA PaCa-2, SCC-25, and UPCI:SCC154 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). .. SCC-25 (ATCC® CRL-1628™) was maintained in a complete Dulbecco’s modified Eagle medium (DMEM)/F12 medium (1:1), while UPCI:SCC154 (ATCC® CRL-3241™) and MIA PaCa-2 (ATCC® CRM-CRL-1420™) were grown in DMEM from Invitrogen (Carlsbad, CA). .. Both growth mediums were supplemented with 10% fetal bovine serum (FBS), 4 mM L-glutamine, 1 mM sodium pyruvate, 100 U/mL penicillin, and 100 mg/mL streptomycin (Invitrogen).

    Derivative Assay:

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

    Cell Culture:

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

    Article Title: Subcellular Distribution of S-Nitrosylated H-Ras in Differentiated and Undifferentiated PC12 Cells during Hypoxia
    Article Snippet: .. PC12 cell line In our experimental study, we cultured PC12 (Adh; ATCC® CRL1721.1™) cells in T25 flasks (Greiner Bio-One GmbH, Cat. No.: 690 170) in a humidified atmosphere that contained 5% CO 2 at 37˚C in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC® 30-2002™) supplemented with 10% heat- inactivated horse serum (Sigma-Aldrich), 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 IU/ml penicillin and 50 µg/ml gentamycin sulphate. .. For the induction of differentiation, PC12 cells were incubated in low serum-DMEM that consisted of 1% heat-inactivated horse serum and 1% FBS, supplemented with nerve growth factor (NGF) 100 ng/ml for 5 days.

    Article Title: Adipose-derived mesenchymal stem cells promote the malignant phenotype of cervical cancer
    Article Snippet: Our results showed that ADSCs promote cell movement, angiogenesis, migration, and the epithelial–mesenchymal transition (EMT) and increase the malignant properties of CC cells through the positive regulation of NF-kappa B signaling, a pathway involved in initiation, progression and resistance to treatment in various types of cancer. .. Cell culture HeLa, SiHa, CaSki, HaCaT and ADSC were obtained from ATCC (Manassas, VA, USA) and cultured in DMEM medium supplied with 5% fetal bovine serum (FBS) (ATCC, 30-2020) at 37 °C/5% CO2 . .. To grow and expand ADSCs we used a commercially available medium (Mesenchymal Stem Cell Basal Medium, ATCC PCS 500030) containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts.

    Isolation:

    Article Title: Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
    Article Snippet: Murine preosteoblast MC3T3-E1 cells, a generous gift from Dr Renny Franceschi (University of Michigan, Ann Arbor MI) were cultured in alpha minimum essential medium (α-MEM) (without ascorbic acid, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 units/mL penicillin-streptomycin (PS, In-vitrogen) at 37° C and 5% carbon dioxide (CO2 ). .. A mouse clonally derived BMSC line (D1 stem cells, ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle medium (DMEM; ATCC) supplemented with 10% FBS (ATCC) and 100 units/mL PS. hBMSCs were isolated from bone marrow aspirate obtained from NDRI (Philadelphia, PA) using Ficoll-Paque (GE Healthcare, Piscataway, NJ) per the manufacturer's instructions. .. Cells at the low-density interphase were collected, rinsed twice with phosphate buffered saline (PBS), and transferred to tissue culture flasks. hBMSCs were cultured in α-MEM containing 10% FBS and 1% PS.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • ls174t  (ATCC)
    99
    ATCC ls174t
    EC958 adhesion and invasion of mucus-producing <t>LS174T</t> human colorectal epithelial cells and human intestinal biopsy specimens. A , LS174T monolayers were incubated with EC958 wild-type (WT) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria). Micrographs depict immunofluorescence staining of monolayers (n = 5 in duplicate); MUC2 is stained green, and red is stained EC958 WT. Scale bar, 20 µm. B and C , Immunofluorescence staining of human colonic ( B ) and ileal ( C ) biopsy specimens infected with EC958 WT for 7 hours with corresponding non-infected controls (n = 2 in duplicate). Tissue specimens were stained for actin (green) and EC958 (red). Scale bar, 50 µm. D , Human ileal biopsy specimens infected with EC958 for 8 hours (n = 2 in duplicate). Adherent EC958 (red) are present on exposed submucosal tissue (villus on right, indicated with white arrows) but not on intact epithelium (villus on left), which is indicated by an actin-rich (green) brush border. Cell nuclei are counterstained in blue.
    Ls174t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ls174t/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ls174t - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    nih3t3  (ATCC)
    99
    ATCC nih3t3
    Energy and wavelength requirement for PIP2 solubilization by photoexcited retinal. ( A ) UV-VIS absorption spectra for both 11-cis retinal (11CR) and all trans retinal (ATR). Note that 445 nm blue light spectrally overlaps with both absorption spectra. ( B ) The absorption spectra of melanopsin and retinal (left), (ε ATR = 44180.0 M −1 cm −1 ). ( C ) The energy level diagram and the population (pop.) of energy levels of free retinal (blue) and melanopsin (red) according to their respective absorption maxima (right). Note that blue light (445 nm) can highly populate melanopsin compared to that of free retinal. ( D – F ) Images of HeLa cells expressing PIP2 sensor (mCherry-PH). ( D ) Cells were incubated with ATR (50 µM) for 5 minutes. A substantial PIP2 sensor translocation was observed upon exposing cells to short pulses of blue light (4.86 µW of 445 nm). The plot shows the dynamics of PIP2 sensor translocation to cytosol. ( E ) In the absence of retinal, cells did not show a detectable PIP2 sensor translocation when exposed to blue light or other wavelengths. ( F ) Both blue light excited ATR (50 µM) and 11CR (50 µM) exhibited a permanent accumulation of PIP2 sensor cytosol. Compared to exposed cell (yellow arrow), control cell without blue light (BL) exposure (white arrow) did not show any detectable PIP2 response. The plots show the dynamics of PIP2 sensor translocation in cells shown in F (mean ± S.E.M., n = 6 cells). ( G ) All trans retinal and blue light induce PIP2 sensor translocation in cells with distinct origins. Images of RAW264.7, <t>NIH3t3,</t> ARPE-19, MDA-MB-468, BT-20, HCT116 and HEK293 cells expressing mCherry-PH (PIP2 sensor). ATR (50 µM) was incubated in cells for 5 minutes followed by continuous exposure of blue light for 5 minutes. Blue light exposure induced PIP2 sensor translocation from PM in all the cell types tested while cells that were not exposed to blue light did not respond. Mean and S.E.M. are from 3
    Nih3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nih3t3/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nih3t3 - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    99
    ATCC wm266 4 melanoma
    Representative dynamic spectra from a <t>WM266.4</t> melanoma cell suspension. Kinetic modeling was performed using a 2-site (left) and 3-site (right) model. Total (T), intracellular (I) and extracellular (E) [1- 13 C]lactate fits, derived from the 3-site kinetic model are shown. Residuals between the data and the model are shown (central row). The concentration curves (bottom) were generated by correcting data for hyperpolarized relaxation.
    Wm266 4 Melanoma, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wm266 4 melanoma/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wm266 4 melanoma - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    EC958 adhesion and invasion of mucus-producing LS174T human colorectal epithelial cells and human intestinal biopsy specimens. A , LS174T monolayers were incubated with EC958 wild-type (WT) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria). Micrographs depict immunofluorescence staining of monolayers (n = 5 in duplicate); MUC2 is stained green, and red is stained EC958 WT. Scale bar, 20 µm. B and C , Immunofluorescence staining of human colonic ( B ) and ileal ( C ) biopsy specimens infected with EC958 WT for 7 hours with corresponding non-infected controls (n = 2 in duplicate). Tissue specimens were stained for actin (green) and EC958 (red). Scale bar, 50 µm. D , Human ileal biopsy specimens infected with EC958 for 8 hours (n = 2 in duplicate). Adherent EC958 (red) are present on exposed submucosal tissue (villus on right, indicated with white arrows) but not on intact epithelium (villus on left), which is indicated by an actin-rich (green) brush border. Cell nuclei are counterstained in blue.

    Journal: The Journal of Infectious Diseases

    Article Title: Intestinal Colonization Traits of Pandemic Multidrug-Resistant Escherichia coli ST131

    doi: 10.1093/infdis/jiy031

    Figure Lengend Snippet: EC958 adhesion and invasion of mucus-producing LS174T human colorectal epithelial cells and human intestinal biopsy specimens. A , LS174T monolayers were incubated with EC958 wild-type (WT) for 1 hour (to determine the number of colony-forming units [CFU] of adherent bacteria) and then treated with gentamicin for 1 hour (to determine the number of CFUs of intracellular bacteria). Micrographs depict immunofluorescence staining of monolayers (n = 5 in duplicate); MUC2 is stained green, and red is stained EC958 WT. Scale bar, 20 µm. B and C , Immunofluorescence staining of human colonic ( B ) and ileal ( C ) biopsy specimens infected with EC958 WT for 7 hours with corresponding non-infected controls (n = 2 in duplicate). Tissue specimens were stained for actin (green) and EC958 (red). Scale bar, 50 µm. D , Human ileal biopsy specimens infected with EC958 for 8 hours (n = 2 in duplicate). Adherent EC958 (red) are present on exposed submucosal tissue (villus on right, indicated with white arrows) but not on intact epithelium (villus on left), which is indicated by an actin-rich (green) brush border. Cell nuclei are counterstained in blue.

    Article Snippet: Epithelial Cell Adhesion and Invasion Assays Intestinal epithelial cells Caco-2 (ATCC HTB-37; in Dulbecco’s modified Eagle’s medium [DMEM]), T84 (ATCC CCL-248; in DMEM and Ham’s F-12 nutrient mixture), and LS174T (ATCC CL-188; in DMEM) were maintained in medium (Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen).

    Techniques: Incubation, Immunofluorescence, Staining, Infection

    Energy and wavelength requirement for PIP2 solubilization by photoexcited retinal. ( A ) UV-VIS absorption spectra for both 11-cis retinal (11CR) and all trans retinal (ATR). Note that 445 nm blue light spectrally overlaps with both absorption spectra. ( B ) The absorption spectra of melanopsin and retinal (left), (ε ATR = 44180.0 M −1 cm −1 ). ( C ) The energy level diagram and the population (pop.) of energy levels of free retinal (blue) and melanopsin (red) according to their respective absorption maxima (right). Note that blue light (445 nm) can highly populate melanopsin compared to that of free retinal. ( D – F ) Images of HeLa cells expressing PIP2 sensor (mCherry-PH). ( D ) Cells were incubated with ATR (50 µM) for 5 minutes. A substantial PIP2 sensor translocation was observed upon exposing cells to short pulses of blue light (4.86 µW of 445 nm). The plot shows the dynamics of PIP2 sensor translocation to cytosol. ( E ) In the absence of retinal, cells did not show a detectable PIP2 sensor translocation when exposed to blue light or other wavelengths. ( F ) Both blue light excited ATR (50 µM) and 11CR (50 µM) exhibited a permanent accumulation of PIP2 sensor cytosol. Compared to exposed cell (yellow arrow), control cell without blue light (BL) exposure (white arrow) did not show any detectable PIP2 response. The plots show the dynamics of PIP2 sensor translocation in cells shown in F (mean ± S.E.M., n = 6 cells). ( G ) All trans retinal and blue light induce PIP2 sensor translocation in cells with distinct origins. Images of RAW264.7, NIH3t3, ARPE-19, MDA-MB-468, BT-20, HCT116 and HEK293 cells expressing mCherry-PH (PIP2 sensor). ATR (50 µM) was incubated in cells for 5 minutes followed by continuous exposure of blue light for 5 minutes. Blue light exposure induced PIP2 sensor translocation from PM in all the cell types tested while cells that were not exposed to blue light did not respond. Mean and S.E.M. are from 3

    Journal: Scientific Reports

    Article Title: Blue light excited retinal intercepts cellular signaling

    doi: 10.1038/s41598-018-28254-8

    Figure Lengend Snippet: Energy and wavelength requirement for PIP2 solubilization by photoexcited retinal. ( A ) UV-VIS absorption spectra for both 11-cis retinal (11CR) and all trans retinal (ATR). Note that 445 nm blue light spectrally overlaps with both absorption spectra. ( B ) The absorption spectra of melanopsin and retinal (left), (ε ATR = 44180.0 M −1 cm −1 ). ( C ) The energy level diagram and the population (pop.) of energy levels of free retinal (blue) and melanopsin (red) according to their respective absorption maxima (right). Note that blue light (445 nm) can highly populate melanopsin compared to that of free retinal. ( D – F ) Images of HeLa cells expressing PIP2 sensor (mCherry-PH). ( D ) Cells were incubated with ATR (50 µM) for 5 minutes. A substantial PIP2 sensor translocation was observed upon exposing cells to short pulses of blue light (4.86 µW of 445 nm). The plot shows the dynamics of PIP2 sensor translocation to cytosol. ( E ) In the absence of retinal, cells did not show a detectable PIP2 sensor translocation when exposed to blue light or other wavelengths. ( F ) Both blue light excited ATR (50 µM) and 11CR (50 µM) exhibited a permanent accumulation of PIP2 sensor cytosol. Compared to exposed cell (yellow arrow), control cell without blue light (BL) exposure (white arrow) did not show any detectable PIP2 response. The plots show the dynamics of PIP2 sensor translocation in cells shown in F (mean ± S.E.M., n = 6 cells). ( G ) All trans retinal and blue light induce PIP2 sensor translocation in cells with distinct origins. Images of RAW264.7, NIH3t3, ARPE-19, MDA-MB-468, BT-20, HCT116 and HEK293 cells expressing mCherry-PH (PIP2 sensor). ATR (50 µM) was incubated in cells for 5 minutes followed by continuous exposure of blue light for 5 minutes. Blue light exposure induced PIP2 sensor translocation from PM in all the cell types tested while cells that were not exposed to blue light did not respond. Mean and S.E.M. are from 3

    Article Snippet: RAW264.7 (RPMI/10% DFBS/1% PS), NIH3T3 (DMEM/10% BCS/ 1% PS), HEK293 (DMEM/10% DFBS/1% PS), MDA-MB-468, HCT116 and BT20 (DMEM/10% FBS/1% PS) and ARPE-19 (DMEM-F12 (50:50)/10% FBS/1% PS) (ATCC, Manassas, VA).

    Techniques: Expressing, Incubation, Translocation Assay, Multiple Displacement Amplification

    Representative dynamic spectra from a WM266.4 melanoma cell suspension. Kinetic modeling was performed using a 2-site (left) and 3-site (right) model. Total (T), intracellular (I) and extracellular (E) [1- 13 C]lactate fits, derived from the 3-site kinetic model are shown. Residuals between the data and the model are shown (central row). The concentration curves (bottom) were generated by correcting data for hyperpolarized relaxation.

    Journal: PLoS ONE

    Article Title: Model Free Approach to Kinetic Analysis of Real-Time Hyperpolarized 13C Magnetic Resonance Spectroscopy Data

    doi: 10.1371/journal.pone.0071996

    Figure Lengend Snippet: Representative dynamic spectra from a WM266.4 melanoma cell suspension. Kinetic modeling was performed using a 2-site (left) and 3-site (right) model. Total (T), intracellular (I) and extracellular (E) [1- 13 C]lactate fits, derived from the 3-site kinetic model are shown. Residuals between the data and the model are shown (central row). The concentration curves (bottom) were generated by correcting data for hyperpolarized relaxation.

    Article Snippet: Cell lines : CHL-1 melanoma (from American Type Culture Collection, ATCC, cultured in Dulbecco's Modified Eagle Medium (DMEM) containing glutamine and 1% non-essential amino acids), HCT116 Bax-KO colon carcinoma (a kind gift from Dr. Bert Vogelstein, Johns Hopkins Medical Center, USA; via Dr. Paul Clarke, ICR, Sutton, UK , cultured in DMEM with glutamine and 1% non-essential amino acids (Invitrogen, UK)), HT29 colon carcinoma (from American Type Culture Collection, ATCC, cultured in McCoy 5A Medium with glutamine and HEPES (Invitrogen, UK)), PC3 prostate adenocarcinoma (from American Type Culture Collection, ATCC, cultured in DMEM with glutamine (Invitrogen, UK)), pediatric SF188 glioblastoma (a kind gift from Dr. Daphne Haas-Kogan, UCSF, San Francisco, CA, USA , cultured in DMEM/F12 Ham's medium (Invitrogen, UK), without penicillin & streptomycin), SW1222 colon carcinoma (from The European Collection of Cell Cultures, ECACC, cultured in DMEM with glutamine (Invitrogen, UK)), WM266-4 melanoma (from American Type Culture Collection, ATCC, cultured in DMEM containing glutamine and 1% non-essential amino acids).

    Techniques: Derivative Assay, Concentration Assay, Generated