dulbecco s modified eagle medium  (Thermo Fisher)


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    Name:
    Dulbecco s Modified Eagle s Limiting Medium DMEM LM
    Description:
    Formulation Sterile filtered DMEM L leucine L methionine with 4 5g L glucose 4 0mM L glutamine sodium pyruvate and phenol red Related Products L Photo Leucine L Photo Methionine
    Catalog Number:
    30030
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Protein Biology|Protein Crosslinking|Protein Labeling & Crosslinking
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    Structured Review

    Thermo Fisher dulbecco s modified eagle medium
    Formulation Sterile filtered DMEM L leucine L methionine with 4 5g L glucose 4 0mM L glutamine sodium pyruvate and phenol red Related Products L Photo Leucine L Photo Methionine
    https://www.bioz.com/result/dulbecco s modified eagle medium/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle medium - by Bioz Stars, 2021-03
    86/100 stars

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    Modification:

    Article Title: Modulation of ERK1/2 and Akt Pathways Involved in the Neurotrophic Action of Caffeic Acid Alkyl Esters
    Article Snippet: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), rat nerve growth factor (NGF-β) and all other reagents needed for chemical synthesis were obtained from Sigma-Aldrich (St Louis, MO, USA). .. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and horse serum were acquired from Invitrogen (San Diego, CA, USA), while penicillin/streptomycin, RPMI 1640, sterile phosphate-buffered saline (PBS), and trypsin EDTA 0.25% were purchased from Biosera (Ringmer, UK). .. Anti-phospho-TrkA, anti-MAP kinase ERK1/2, anti-phospho-MAP kinase ERK1/2 (pThr202/Tyr204), and anti-phospho-Akt antibodies were obtained from Cell Signaling (Danvers, MA, USA).

    Article Title: Structure–Activity Relationship of Piplartine and Synthetic Analogues against Schistosoma mansoni and Cytotoxicity to Mammalian Cells
    Article Snippet: Murine fibroblast (NIH3T3) cells were acquired from the cell bank of Rio de Janeiro (Rio de Janeiro, Brazil), and the murine BALB/cN macrophage (J774A.1, TIB-67™) (ATCC® , Washington, DC, USA). .. Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco BRL, Grand Island, NY, USA) supplemented with 10% heat inactivated foetal bovine serum (Life, USA) and 1% antibiotic solution (100 IU/mL penicillin/100 µg/mL streptomycin, Life, USA) at 37 °C and 5% CO2 . .. Viability assays were performed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) dye reduction method.

    Article Title: Induction of sarcomas by mutant IDH2
    Article Snippet: All sequences were confirmed by direct sequencing before retrovirus generation. .. C3H10T1/2 cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Invitrogen) with 10% fetal bovine serum (FBS; CellGro). .. To generate 10T cell lines with stable expression of wild-type or mutant IDH2, supernatant from 293T cells transfected with pCL-Eco helper virus and plasmids was collected after 72 h, filtered, and applied to 10T parental cells overnight.

    Article Title: Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells
    Article Snippet: Culture of rat bone marrow MSCs Bone marrow MSCs were isolated and cultured from 6-week-old male F344 rats according to our previously published procedures. .. Briefly, the bone marrow was rinsed using Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY) with 10% fetal bovine serum (Hyclone, Logan, UT) and 200 U/mL heparin (Sigma, St Louis, MO) from rat femurs after both ends were cut off at the epiphysis. .. Cells were cultured in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum in an incubator with an atmosphere of 5% CO2 at 37°C.

    Article Title: Melatonin inhibits the proliferation of breast cancer cells induced by bisphenol A via targeting estrogen receptor‐related pathways
    Article Snippet: Rabbit primary monoclonal antibodies against SRCs, AKT, ERK1/2, phospho‐AKTSer473 , phospho‐ERK1/2, p21, GAPDH, and anti‐goat and anti‐rabbit immunoglobulin G horseradish peroxidase‐linked secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Cell culture MCF‐7 and T47D cell lines were acquired from the American Type Culture Collection (ATCC, Rockville, MD, USA), and cultivated in 10% fetal bovine serum (FBS) supplemented Dulbecco's modified Eagle's medium (DMEM) or RPMI 1640 media (Gibco, Rockville, MD, USA) containing penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37°C with 5% CO2 . .. Plasmids The pERE‐E1b‐luc reporter plasmid, which contains the vitellogenin ERE, and pCMV‐[beta]‐galactosidase (pCMV‐[beta]‐gal) were kindly provided by C. Smith (Baylor College of Medicine).

    Cell Culture:

    Article Title: Structure–Activity Relationship of Piplartine and Synthetic Analogues against Schistosoma mansoni and Cytotoxicity to Mammalian Cells
    Article Snippet: Murine fibroblast (NIH3T3) cells were acquired from the cell bank of Rio de Janeiro (Rio de Janeiro, Brazil), and the murine BALB/cN macrophage (J774A.1, TIB-67™) (ATCC® , Washington, DC, USA). .. Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco BRL, Grand Island, NY, USA) supplemented with 10% heat inactivated foetal bovine serum (Life, USA) and 1% antibiotic solution (100 IU/mL penicillin/100 µg/mL streptomycin, Life, USA) at 37 °C and 5% CO2 . .. Viability assays were performed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) dye reduction method.

    Article Title: Meso-Endothelial Bipotent Progenitors from Human Placenta Display Distinct Molecular and Cellular Identity
    Article Snippet: .. Single-cell suspensions upon digestion were denoted “unsorted population” and cultured in DMEM (Gibco, USA) supplemented with 10% FBS (Gibco), or in the endothelial cell growth medium (EGM2; Lonza, USA). .. For removal of hematopoietic populations, unsorted cells were resuspended in ice-cold MACS buffer (PBS [Lonza]/0.5% BSA [Sigma]/2.5 mM EDTA disodium salt [Merck, Darmstadt, Germany]) and incubated in Dynabeads CD45 (Invitrogen, USA) for 20 min and the CD45+ cells depleted by using a magnet (DynaMag-15; Invitrogen).

    Article Title: STAT1 deficiency supports PD-1/PD-L1 signaling resulting in dysfunctional TNFα mediated immune responses in a model of NSCLC
    Article Snippet: .. LL/2-luc-M38 cells were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FCS, 1% Pen/Strep and 1% l-Glut at 37°C and 5% CO2 . .. Mycoplasma contamination was detected using Mycoplasma Detection Kit (Absource Diagnostics GmbH) according to the manufacturer’s instructions (latest test: August, 9th, 2016).

    Article Title: Induction of sarcomas by mutant IDH2
    Article Snippet: All sequences were confirmed by direct sequencing before retrovirus generation. .. C3H10T1/2 cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Invitrogen) with 10% fetal bovine serum (FBS; CellGro). .. To generate 10T cell lines with stable expression of wild-type or mutant IDH2, supernatant from 293T cells transfected with pCL-Eco helper virus and plasmids was collected after 72 h, filtered, and applied to 10T parental cells overnight.

    Article Title: Melatonin inhibits the proliferation of breast cancer cells induced by bisphenol A via targeting estrogen receptor‐related pathways
    Article Snippet: Rabbit primary monoclonal antibodies against SRCs, AKT, ERK1/2, phospho‐AKTSer473 , phospho‐ERK1/2, p21, GAPDH, and anti‐goat and anti‐rabbit immunoglobulin G horseradish peroxidase‐linked secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Cell culture MCF‐7 and T47D cell lines were acquired from the American Type Culture Collection (ATCC, Rockville, MD, USA), and cultivated in 10% fetal bovine serum (FBS) supplemented Dulbecco's modified Eagle's medium (DMEM) or RPMI 1640 media (Gibco, Rockville, MD, USA) containing penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37°C with 5% CO2 . .. Plasmids The pERE‐E1b‐luc reporter plasmid, which contains the vitellogenin ERE, and pCMV‐[beta]‐galactosidase (pCMV‐[beta]‐gal) were kindly provided by C. Smith (Baylor College of Medicine).

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  • 99
    Thermo Fisher dmem f
    Reconstitution of signal-dependent retrieval of SSTR3 and GPR161. (A) Diagram of the signal-dependent retrieval systems under study. Left: Addition of sst triggers SSTR3 exit from cilia by directly activating SSTR3. Right: Addition of SAG activates the Hedgehog pathway and promotes GPR161 retrieval. SMO, Smoothened. (B) Kinetics of SSTR3 disappearance from cilia of primary hippocampal neurons and of IMCD3 stably expressing AP SSTR3 NG under the control of the TATA-less EF1α promoter were estimated by quantitation of immunofluorescence signals after addition of sst. The entire dataset for the sst condition is shown in Fig. S1 B. Data were fitted to a single exponential. Error bars indicate 95% confidence interval (CI). n = 280–424 cilia (neurons) and 57–80 cilia (IMCD3). (C) High-level expression of SSTR3 drives elongation of primary cilia. Top: AP SSTR3 GFP driven by various promoters or AP SSTR3 NG ) or an NG calibrator (see Materials and methods). Endogenous SSTR3 levels were estimated by comparative immunostaining (see Materials and methods). A Mann-Whitney test was used for pairwise comparisons of the number of SSTR3 molecules per cilia in neurons and in IMCD3 cells expressing AP SSTR3 NG or AP SSTR3 GFP under the control of pEF1αΔ. P > 0.05. n = 10–38 cilia. Error bars represent SD. Bottom: Effect of AP SSTR3 GFP expression on cilium length. Cilia lengths were measured in the GFP channel by live-cell imaging. n . (D) IMCD3-[pCrys- AP GPR161 NG3 ] were treated for 2 h with either SAG or vehicle. AP GPR161 NG3 was visualized by NG fluorescence, and basal bodies of cilia were stained with ninein. All cells were pretreated with the translation inhibitor emetine to eliminate signals from new protein synthesis. Bar, 4 µm. (E) Absolute quantitation of ciliary GPCR abundance. Top: Calibration of single-molecule fluorescence intensity. Bacterially expressed NG3 protein was spotted on glass coverslips (inset), and the fluorescent intensity of each individual NG3 was measured. n = 1,257 particles measured. Bottom: The three-step photobleaching of a representative spot shows that the fluorescence was emitted by a single NG3 molecule. The measured fluorescence intensity of NG3 was used to calibrate NG- and NG3-tagged SSTR3, GPR161, BBS5, and IFT88. Bar, 0.5 μm. (F) IMCD3-[pEF1αΔ- AP SSTR3 NG ] cells were treated with vehicle or sst for 2 h. Stable expression of an ER-localized biotin ligase BirA enables the biotinylation of AP SSTR3 with the biotin existing in the <t>DMEM/F-12</t> cell culture medium. Ciliary AP SSTR3 was pulse-labeled by Alexa Fluor 647–conjugated mSA (mSA647) for 5–10 min before imaging (see Materials and methods for details). Bar, 1 μm. The absolute number of AP SSTR3 NG molecules per cilia at t 0 was calculated by measuring the NG signal and using the NG3 calibrator. For all other time points, the ratio in ciliary mSA647 signal compared with t 0 was used to calculate the absolute number of molecules (see Materials and methods for details). Data were fitted to a single exponential. Error bars indicate 95% CI. n = 14 cilia. (G) IMCD3-[pCrys-GPR161 NG3 ] cells were treated with SAG or vehicle for 2 h. NG fluorescence was tracked in individual cilia, and the ratio of GPR161 NG3 to endogenous GPR161 was used to calculate the total levels of GPR161 as detailed in Materials and methods. Bar, 1 μm. Data were fitted to a single exponential. Error bars indicate 95% CI. n = 12–20 cilia.
    Dmem F, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher minimal essential medium dmem
    Plaque morphology and growth kinetics of WT and NS2A/A30P mutated WNV KUN in BHK-21 and <t>A549</t> cells. (A) Immunofluorescence analysis of viral foci. BHK-21 and A549 cells on coverslips in 24-well plates were infected with the WT or NS2A/A30P mutated WNV KUN and overlaid with 0.75% LMT agarose in <t>DMEM</t> containing 2% FBS. At 48 h after infection, the cells were fixed and stained using a monoclonal antibody specific for the WNV E protein. (B) Plaque assay. BHK and A549 cells in six-well plates were infected with the WT or NS2A/A30P-mutated WNV KUN , overlaid with 0.75% LMT agarose in DMEM with 2% FBS, and stained with crystal violet at 5 days postinfection. (C and D) Virus growth kinetics. BHK-21 and A549 cells in six-well plates were infected with the WT or NS2A/A30P-mutated WNV KUN at an MOI of ∼1. At the indicated times, cell culture fluid was collected and virus titers in the cell culture fluid were determined by plaque assays on BHK-21 cells.
    Minimal Essential Medium Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Reconstitution of signal-dependent retrieval of SSTR3 and GPR161. (A) Diagram of the signal-dependent retrieval systems under study. Left: Addition of sst triggers SSTR3 exit from cilia by directly activating SSTR3. Right: Addition of SAG activates the Hedgehog pathway and promotes GPR161 retrieval. SMO, Smoothened. (B) Kinetics of SSTR3 disappearance from cilia of primary hippocampal neurons and of IMCD3 stably expressing AP SSTR3 NG under the control of the TATA-less EF1α promoter were estimated by quantitation of immunofluorescence signals after addition of sst. The entire dataset for the sst condition is shown in Fig. S1 B. Data were fitted to a single exponential. Error bars indicate 95% confidence interval (CI). n = 280–424 cilia (neurons) and 57–80 cilia (IMCD3). (C) High-level expression of SSTR3 drives elongation of primary cilia. Top: AP SSTR3 GFP driven by various promoters or AP SSTR3 NG ) or an NG calibrator (see Materials and methods). Endogenous SSTR3 levels were estimated by comparative immunostaining (see Materials and methods). A Mann-Whitney test was used for pairwise comparisons of the number of SSTR3 molecules per cilia in neurons and in IMCD3 cells expressing AP SSTR3 NG or AP SSTR3 GFP under the control of pEF1αΔ. P > 0.05. n = 10–38 cilia. Error bars represent SD. Bottom: Effect of AP SSTR3 GFP expression on cilium length. Cilia lengths were measured in the GFP channel by live-cell imaging. n . (D) IMCD3-[pCrys- AP GPR161 NG3 ] were treated for 2 h with either SAG or vehicle. AP GPR161 NG3 was visualized by NG fluorescence, and basal bodies of cilia were stained with ninein. All cells were pretreated with the translation inhibitor emetine to eliminate signals from new protein synthesis. Bar, 4 µm. (E) Absolute quantitation of ciliary GPCR abundance. Top: Calibration of single-molecule fluorescence intensity. Bacterially expressed NG3 protein was spotted on glass coverslips (inset), and the fluorescent intensity of each individual NG3 was measured. n = 1,257 particles measured. Bottom: The three-step photobleaching of a representative spot shows that the fluorescence was emitted by a single NG3 molecule. The measured fluorescence intensity of NG3 was used to calibrate NG- and NG3-tagged SSTR3, GPR161, BBS5, and IFT88. Bar, 0.5 μm. (F) IMCD3-[pEF1αΔ- AP SSTR3 NG ] cells were treated with vehicle or sst for 2 h. Stable expression of an ER-localized biotin ligase BirA enables the biotinylation of AP SSTR3 with the biotin existing in the DMEM/F-12 cell culture medium. Ciliary AP SSTR3 was pulse-labeled by Alexa Fluor 647–conjugated mSA (mSA647) for 5–10 min before imaging (see Materials and methods for details). Bar, 1 μm. The absolute number of AP SSTR3 NG molecules per cilia at t 0 was calculated by measuring the NG signal and using the NG3 calibrator. For all other time points, the ratio in ciliary mSA647 signal compared with t 0 was used to calculate the absolute number of molecules (see Materials and methods for details). Data were fitted to a single exponential. Error bars indicate 95% CI. n = 14 cilia. (G) IMCD3-[pCrys-GPR161 NG3 ] cells were treated with SAG or vehicle for 2 h. NG fluorescence was tracked in individual cilia, and the ratio of GPR161 NG3 to endogenous GPR161 was used to calculate the total levels of GPR161 as detailed in Materials and methods. Bar, 1 μm. Data were fitted to a single exponential. Error bars indicate 95% CI. n = 12–20 cilia.

    Journal: The Journal of Cell Biology

    Article Title: BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone

    doi: 10.1083/jcb.201709041

    Figure Lengend Snippet: Reconstitution of signal-dependent retrieval of SSTR3 and GPR161. (A) Diagram of the signal-dependent retrieval systems under study. Left: Addition of sst triggers SSTR3 exit from cilia by directly activating SSTR3. Right: Addition of SAG activates the Hedgehog pathway and promotes GPR161 retrieval. SMO, Smoothened. (B) Kinetics of SSTR3 disappearance from cilia of primary hippocampal neurons and of IMCD3 stably expressing AP SSTR3 NG under the control of the TATA-less EF1α promoter were estimated by quantitation of immunofluorescence signals after addition of sst. The entire dataset for the sst condition is shown in Fig. S1 B. Data were fitted to a single exponential. Error bars indicate 95% confidence interval (CI). n = 280–424 cilia (neurons) and 57–80 cilia (IMCD3). (C) High-level expression of SSTR3 drives elongation of primary cilia. Top: AP SSTR3 GFP driven by various promoters or AP SSTR3 NG ) or an NG calibrator (see Materials and methods). Endogenous SSTR3 levels were estimated by comparative immunostaining (see Materials and methods). A Mann-Whitney test was used for pairwise comparisons of the number of SSTR3 molecules per cilia in neurons and in IMCD3 cells expressing AP SSTR3 NG or AP SSTR3 GFP under the control of pEF1αΔ. P > 0.05. n = 10–38 cilia. Error bars represent SD. Bottom: Effect of AP SSTR3 GFP expression on cilium length. Cilia lengths were measured in the GFP channel by live-cell imaging. n . (D) IMCD3-[pCrys- AP GPR161 NG3 ] were treated for 2 h with either SAG or vehicle. AP GPR161 NG3 was visualized by NG fluorescence, and basal bodies of cilia were stained with ninein. All cells were pretreated with the translation inhibitor emetine to eliminate signals from new protein synthesis. Bar, 4 µm. (E) Absolute quantitation of ciliary GPCR abundance. Top: Calibration of single-molecule fluorescence intensity. Bacterially expressed NG3 protein was spotted on glass coverslips (inset), and the fluorescent intensity of each individual NG3 was measured. n = 1,257 particles measured. Bottom: The three-step photobleaching of a representative spot shows that the fluorescence was emitted by a single NG3 molecule. The measured fluorescence intensity of NG3 was used to calibrate NG- and NG3-tagged SSTR3, GPR161, BBS5, and IFT88. Bar, 0.5 μm. (F) IMCD3-[pEF1αΔ- AP SSTR3 NG ] cells were treated with vehicle or sst for 2 h. Stable expression of an ER-localized biotin ligase BirA enables the biotinylation of AP SSTR3 with the biotin existing in the DMEM/F-12 cell culture medium. Ciliary AP SSTR3 was pulse-labeled by Alexa Fluor 647–conjugated mSA (mSA647) for 5–10 min before imaging (see Materials and methods for details). Bar, 1 μm. The absolute number of AP SSTR3 NG molecules per cilia at t 0 was calculated by measuring the NG signal and using the NG3 calibrator. For all other time points, the ratio in ciliary mSA647 signal compared with t 0 was used to calculate the absolute number of molecules (see Materials and methods for details). Data were fitted to a single exponential. Error bars indicate 95% CI. n = 14 cilia. (G) IMCD3-[pCrys-GPR161 NG3 ] cells were treated with SAG or vehicle for 2 h. NG fluorescence was tracked in individual cilia, and the ratio of GPR161 NG3 to endogenous GPR161 was used to calculate the total levels of GPR161 as detailed in Materials and methods. Bar, 1 μm. Data were fitted to a single exponential. Error bars indicate 95% CI. n = 12–20 cilia.

    Article Snippet: Cells were imaged in DMEM/F-12 media with Hepes, no phenol red, and 0.2% FBS (11039-021; Gibco).

    Techniques: Stable Transfection, Expressing, Quantitation Assay, Immunofluorescence, Immunostaining, MANN-WHITNEY, Live Cell Imaging, Fluorescence, Staining, Cell Culture, Labeling, Imaging

    A NPC‐based phenotypic screening platform to discover inducers of OL differentiation and maturation. (a) Steps of OL differentiation from neurospheres generated from cortical NPCs of mouse E14.5 embryos: Neurosphere formation (NPC medium), OPC differentiation (OPC medium), and OL differentiation and maturation (OL medium). (b) Phase contrast (Top) and immunofluorescent staining of specific markers (Bottom) of NPCs (Nestin), OPCs (NG2), and OLs (MBP). Nuclei were stained with Hoechst. Scale bars, 40 μm. (c) The negative (DMSO) and positive (T3, 100 nM) controls of the high‐throughput screening system (Left). Scatter plot of the primary screen results of 7347 compounds (Right). The dotted line indicates two‐fold increase in the percentage of MBP + cells comparing to DMSO. (d) Representative images of MBP + ]

    Journal: Glia

    Article Title: Vitamin C promotes oligodendrocytes generation and remyelination. Vitamin C promotes oligodendrocytes generation and remyelination

    doi: 10.1002/glia.23306

    Figure Lengend Snippet: A NPC‐based phenotypic screening platform to discover inducers of OL differentiation and maturation. (a) Steps of OL differentiation from neurospheres generated from cortical NPCs of mouse E14.5 embryos: Neurosphere formation (NPC medium), OPC differentiation (OPC medium), and OL differentiation and maturation (OL medium). (b) Phase contrast (Top) and immunofluorescent staining of specific markers (Bottom) of NPCs (Nestin), OPCs (NG2), and OLs (MBP). Nuclei were stained with Hoechst. Scale bars, 40 μm. (c) The negative (DMSO) and positive (T3, 100 nM) controls of the high‐throughput screening system (Left). Scatter plot of the primary screen results of 7347 compounds (Right). The dotted line indicates two‐fold increase in the percentage of MBP + cells comparing to DMSO. (d) Representative images of MBP + ]

    Article Snippet: NPCs were expanded as neurospheres in the NPC medium (DMEM/F12 (Gibco), 20 ng mL−1 EGF, 20 ng mL−1 bFGF, 2% B27 (Invitrogen), 100 U mL−1 penicillin, and 100 μg mL−1 streptomycin).

    Techniques: Generated, Staining, High Throughput Screening Assay

    Comparison of IVM for COCs cultured in the same media: DMEM-F12 (a), G1-PLUS (b), G2-PLUS (c), and TYH (d). The data were analyzed by the chi-squared test. A value of P

    Journal: Stem Cells International

    Article Title: Differentiation Potential of Human Wharton's Jelly-Derived Mesenchymal Stem Cells and Paracrine Signaling Interaction Contribute to Improve the In Vitro Maturation of Mouse Cumulus Oocyte Complexes

    doi: 10.1155/2018/7609284

    Figure Lengend Snippet: Comparison of IVM for COCs cultured in the same media: DMEM-F12 (a), G1-PLUS (b), G2-PLUS (c), and TYH (d). The data were analyzed by the chi-squared test. A value of P

    Article Snippet: The third experimental stage included the following types of coculture with hWJ-MSCs: (i) coculture in DMEM-F12, (j) coculture in G1-PLUS, (k) coculture in G2-PLUS, and (l) coculture in TYH.

    Techniques: Cell Culture

    qPCR analysis of OCT4 in hWJ-MSCs cultured under the 4 coculture conditions. In G1-PLUS, TYH, and G2-PLUS, OCT4 expression was downregulated to 25.9, 24.7, and 6.6%, respectively, compared to the OCT4 level in hWJ-MSCs cultured in DMEM-F12. The expression of OCT4 from hWJ-MSCs was not affected by the presence of COCs when cultured in DMEM-F12 (control group). The data was analyzed with the two-tailed paired Student's t -test. A P value

    Journal: Stem Cells International

    Article Title: Differentiation Potential of Human Wharton's Jelly-Derived Mesenchymal Stem Cells and Paracrine Signaling Interaction Contribute to Improve the In Vitro Maturation of Mouse Cumulus Oocyte Complexes

    doi: 10.1155/2018/7609284

    Figure Lengend Snippet: qPCR analysis of OCT4 in hWJ-MSCs cultured under the 4 coculture conditions. In G1-PLUS, TYH, and G2-PLUS, OCT4 expression was downregulated to 25.9, 24.7, and 6.6%, respectively, compared to the OCT4 level in hWJ-MSCs cultured in DMEM-F12. The expression of OCT4 from hWJ-MSCs was not affected by the presence of COCs when cultured in DMEM-F12 (control group). The data was analyzed with the two-tailed paired Student's t -test. A P value

    Article Snippet: The third experimental stage included the following types of coculture with hWJ-MSCs: (i) coculture in DMEM-F12, (j) coculture in G1-PLUS, (k) coculture in G2-PLUS, and (l) coculture in TYH.

    Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Two Tailed Test

    Plaque morphology and growth kinetics of WT and NS2A/A30P mutated WNV KUN in BHK-21 and A549 cells. (A) Immunofluorescence analysis of viral foci. BHK-21 and A549 cells on coverslips in 24-well plates were infected with the WT or NS2A/A30P mutated WNV KUN and overlaid with 0.75% LMT agarose in DMEM containing 2% FBS. At 48 h after infection, the cells were fixed and stained using a monoclonal antibody specific for the WNV E protein. (B) Plaque assay. BHK and A549 cells in six-well plates were infected with the WT or NS2A/A30P-mutated WNV KUN , overlaid with 0.75% LMT agarose in DMEM with 2% FBS, and stained with crystal violet at 5 days postinfection. (C and D) Virus growth kinetics. BHK-21 and A549 cells in six-well plates were infected with the WT or NS2A/A30P-mutated WNV KUN at an MOI of ∼1. At the indicated times, cell culture fluid was collected and virus titers in the cell culture fluid were determined by plaque assays on BHK-21 cells.

    Journal: Journal of Virology

    Article Title: A Single Amino Acid Substitution in the West Nile Virus Nonstructural Protein NS2A Disables Its Ability To Inhibit Alpha/Beta Interferon Induction and Attenuates Virus Virulence in Mice

    doi: 10.1128/JVI.80.5.2396-2404.2006

    Figure Lengend Snippet: Plaque morphology and growth kinetics of WT and NS2A/A30P mutated WNV KUN in BHK-21 and A549 cells. (A) Immunofluorescence analysis of viral foci. BHK-21 and A549 cells on coverslips in 24-well plates were infected with the WT or NS2A/A30P mutated WNV KUN and overlaid with 0.75% LMT agarose in DMEM containing 2% FBS. At 48 h after infection, the cells were fixed and stained using a monoclonal antibody specific for the WNV E protein. (B) Plaque assay. BHK and A549 cells in six-well plates were infected with the WT or NS2A/A30P-mutated WNV KUN , overlaid with 0.75% LMT agarose in DMEM with 2% FBS, and stained with crystal violet at 5 days postinfection. (C and D) Virus growth kinetics. BHK-21 and A549 cells in six-well plates were infected with the WT or NS2A/A30P-mutated WNV KUN at an MOI of ∼1. At the indicated times, cell culture fluid was collected and virus titers in the cell culture fluid were determined by plaque assays on BHK-21 cells.

    Article Snippet: BHK21, A549, L929, and HEp-2 cells were grown in Dulbecco's modification of minimal essential medium (DMEM) (Invitrogen, Carlsbad, Calif.) supplemented with 10% fetal bovine serum (FBS) at 37°C in a CO2 incubator.

    Techniques: Immunofluorescence, Infection, Staining, Plaque Assay, Cell Culture