dulbecco s modified eagle medium dmem  (Millipore)

 
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    Name:
    Sigmacote
    Description:
    Sigmacote R is a solution of a chlorinated organopolysiloxane in heptane that readily forms a covalent microscopically thin film on glass The film repels water retards the clotting of blood or plasma and prevents surface adsorption of many basic proteins
    Catalog Number:
    sl2
    Price:
    None
    Applications:
    Sigmacote(R) is ideal for glass , ceramics and fiber optics. It is used to treat GC injection glass inserts. Ready to use without dilution; reusable if kept free of moisture. Sigmacote(R) has been used to prevent adhesion of the growth factors to plasticware. It has also been used to coat coverslip in order to prevent the adhesion of larval brain during squashing.
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    Structured Review

    Millipore dulbecco s modified eagle medium dmem
    Sigmacote R is a solution of a chlorinated organopolysiloxane in heptane that readily forms a covalent microscopically thin film on glass The film repels water retards the clotting of blood or plasma and prevents surface adsorption of many basic proteins
    https://www.bioz.com/result/dulbecco s modified eagle medium dmem/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle medium dmem - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Cell Culture:

    Article Title: A novel role for peptidylarginine deiminases in microvesicle release reveals therapeutic potential of PAD inhibition in sensitizing prostate cancer cells to chemotherapyMolecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1alpha,25-dihydroxyvitamin D
    Article Snippet: .. Cell culture The highly metastatic prostate cancer cell line PC3 (Sigma–Aldrich, Gillingham, U.K.) and a control immortalised normal prostate cell line (PNT2; ECACC) were cultured in MV-free complete growth medium (CGM) consisting of EMV (exosome and MV)-free RPMI 1640 supplemented with 10% EMV-free foetal bovine serum (FBS; Hyclone, Thermo Scientific, Paisley, UK) in the absence of antibiotics. .. The CGM medium supplemented with 10% FBS was then centrifuged at 100,000g for 2 h to remove exosomes and MVs before using it in cell culture.

    Article Title: Tyrosinase Inhibition and Kinetic Details of Puerol A Having But-2-Enolide Structure from Amorpha fruticosa
    Article Snippet: The absorbance at 595 nm was measured on a Bio-Rad microplate reader (Hercules, CA, USA). .. Measurement of Melanin Content For the analysis of melanin content accumulated in the cytosol, B16F10 mouse melanoma cells were seeded on to six-well plates at a density of 1 × 105 cells per well, and cultured for 24 h. Then, the cells were treated with 1 μM of α-MSH (Sigma–Aldrich, St. Louis, MO, USA) in the presence or absence of different concentrations of compound 1. .. Thereafter, the cells were collected using trypsin-EDTA (Lonza, Walkersville, MD, USA) and dissolved in 1 N NaOH, including 10% DMSO at 65 °C for 24 h. The melanin content was determined at 415 nm by a Bio-Rad microplate reader [ , ].

    Mouse Assay:

    Article Title: D‐Aspartate treatment attenuates myelin damage and stimulates myelin repair
    Article Snippet: Drugs were applied using a handheld pipette and used at the following concentrations: 1–100 μM AMPA, 100 μM D‐Aspartate, and 10 μM DNQX, 10 μM MK‐801, and 20 μM L‐trans‐pyrrolidine‐2,4‐dicarboxylic acid (PDC). .. Cuprizone‐induced demyelination/remyelination and D‐Asp treatment Experimental toxic demyelination was induced by feeding 8‐week‐old male C57BL/6 mice a diet with 0.2% (w/w) cuprizone (oxalic bis‐cyclohexylidenehydrazide; Sigma‐Aldrich, Milan, Italy) mixed into milled chow pellets (Harlan Laboratories, Milan, Italy). ..

    Concentration Assay:

    Article Title: Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells
    Article Snippet: .. The following ECMPs, GFs, and SMs (Product/Vendor/Catalog #/Concentration) were used: Collagen I/Sigma–Aldrich (St. Louis, MO, United States)/C7774/250 µg/ml, Collagen III/Sigma–Aldrich/C4407/250 µg/ml, Collagen IV/Sigma–Aldrich/C7521/250 µg/ml, Collagen V/Sigma–Aldrich/C3657/250 µg/ml, Fibronectin/Sigma–Aldrich/F2518/250 µg/ml, Laminin/Sigma–Aldrich/L6274/250 µg/ml, Vitronectin/Sigma–Aldrich/V8379/250 µg/ml, Wnt3a/In House/100 ng/ml, R-Spondin/In House/100 ng/ml, CHIR98014/Selleck Chemicals (Houston, TX, United States)/S2745/50 ng/ml, Dkk-1/R & D Systems (Minneapolis, MN, United States)/5439-DK-010/50 ng/ml, IWP-2/Tocris (United Kingdom)/3533/50 ng/ml, FGF/Thermo Fisher Scientific/13256-029/40 ng/ml, KGF/Thermo Fisher Scientific/PHG0094/50 ng/ml, VEGF/R & D Systems/293-VE-010/50 ng/ml, EGF/R & D Systems/236-EG-01M/50 ng/ml, SHH/R & D Systems/464-SH-025/50 ng/ml, Cyclopamine/Tocris/1523/50 ng/ml, BMP4/R & D Systems/314-BP-010/50 ng/ml, Activin/R & D Systems/338-AC-010/50 ng/ml, Dorsomorphin/Sigma–Aldrich/P5499-5MG/50 ng/ml, SB 431542/Tocris/1614/50 ng/ml, Noggin/R & D Systems/6057-NG-025/50 ng/ml. ..

    Article Title: Proteinase activated receptor‐2 counterbalances the vascular effects of endothelin‐1 in fibrotic tight‐skin mice) Proteinase activated receptor‐2 counterbalances the vascular effects of endothelin‐1 in fibrotic tight‐skin mice
    Article Snippet: Conversely, to evaluate tissue vasorelaxation, cumulative concentration–response curves to ACh (10 nM–30 μM; Sigma‐Aldrich) and to the PAR2 tethered ligand peptide (PAR2‐AP, SLIGRL‐NH2 , 1 nM–1 μM; synthesized in house) were performed on rings precontracted with phenylephrine. .. In order to investigate the involvement of NO and COX metabolites, concentration–response curves with ET‐1 or PAR2‐AP were carried out in presence of the NOS inhibitor L‐NG ‐nitro‐arginine methyl ester (L‐NAME, 100 μM, 20 min; Sigma‐Aldrich) and the COX inhibitor ibuprofen (10 μM; Sigma‐Aldrich). (10 μM; Tocris) was used as antagonist for ETA receptors while ENMD1068 was used to block PAR2 (100 μM; Sigma‐Aldrich). .. Samples of the thoracic aorta from six different mouse genotypes were homogenized in lysis buffer containing 0.5 M β‐glycerophosphate, 10 mM sodium orthovanadate, 20 mM MgCl2 , 10 mM EGTA, 100 mM DTT and protease inhibitors.

    Fluorescence:

    Article Title: Lung CSC‐derived exosomal miR‐210‐3p contributes to a pro‐metastatic phenotype in lung cancer by targeting FGFRL1, et al. Lung CSC‐derived exosomal miR‐210‐3p contributes to a pro‐metastatic phenotype in lung cancer by targeting FGFRL1
    Article Snippet: NanoSight NS300 (Malvern Instruments) nanoparticle tracking analysis (NTA) software was then used to visualize and measure particle size. .. 2.11 PKH26 labelling of exosomesTo observe the interaction between exosomes and lung cancer cells, exosomes were labelled with PKH26 (a membrane fluorescence dye that is red in colour) (Sigma‐Aldrich). ..

    Transduction:

    Article Title: Telomere dysfunction promotes transdifferentiation of human fibroblasts into myofibroblasts. Telomere dysfunction promotes transdifferentiation of human fibroblasts into myofibroblasts
    Article Snippet: To measure EdU incorporation, proliferating cells were labeled with 10 μM EdU for 12 hr, and EdU‐positive cells were detected by using the ClickiT EdU Alexa Fluor 488 imaging kit (Invitrogen) according to manufacturer’s instructions. .. BJ and BJ‐hTERT cells were transduced with lentiviral particles encoding shRNAs against TRF2 and p53 (Sigma‐Aldrich, St Louis, MO). .. BJ cells expressing SV40 Large T antigen (Addgene; #1780) and HPV E6 cells were generated by retroviral transductions with pBabe‐Neo‐SV40 Large T antigen plasmid and pBabe‐puro‐HPV‐E6 plasmid, respectively.

    Blocking Assay:

    Article Title: Proteinase activated receptor‐2 counterbalances the vascular effects of endothelin‐1 in fibrotic tight‐skin mice) Proteinase activated receptor‐2 counterbalances the vascular effects of endothelin‐1 in fibrotic tight‐skin mice
    Article Snippet: Conversely, to evaluate tissue vasorelaxation, cumulative concentration–response curves to ACh (10 nM–30 μM; Sigma‐Aldrich) and to the PAR2 tethered ligand peptide (PAR2‐AP, SLIGRL‐NH2 , 1 nM–1 μM; synthesized in house) were performed on rings precontracted with phenylephrine. .. In order to investigate the involvement of NO and COX metabolites, concentration–response curves with ET‐1 or PAR2‐AP were carried out in presence of the NOS inhibitor L‐NG ‐nitro‐arginine methyl ester (L‐NAME, 100 μM, 20 min; Sigma‐Aldrich) and the COX inhibitor ibuprofen (10 μM; Sigma‐Aldrich). (10 μM; Tocris) was used as antagonist for ETA receptors while ENMD1068 was used to block PAR2 (100 μM; Sigma‐Aldrich). .. Samples of the thoracic aorta from six different mouse genotypes were homogenized in lysis buffer containing 0.5 M β‐glycerophosphate, 10 mM sodium orthovanadate, 20 mM MgCl2 , 10 mM EGTA, 100 mM DTT and protease inhibitors.

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  • 97
    Millipore dmem cell culture medium
    Physical-chemical characterization of polystyrene NPs. Intensity weighted size distribution of carboxylated ( a ) and PEGylated ( b ) polystyrene nanoparticles measured by dynamic light scattering. Representative TEM images of the particles are shown in the top right panels of each DLS plot, scale bars represent 400 nm. c , d Aggregation–agglomeration properties and corona thickening of polystyrene nanoparticles was measured by DLS as a change in hydrodynamic size. Particles were incubated in inorganic ( c ) or biological solutions ( d ) for 96 h, and the size distribution was monitored. Color codes of samples are shown in the figure. Data are presented as mean ± standard deviation (n = 3). Particles showed no aggregation in distilled water or in <t>PBS</t> during the 96-h assay period ( c ). In contrast a time-dependent, heavy particle aggregation was found in serum-free <t>DMEM</t> (d). Incubation of nanoparticles with 10 % FBS also evoked an immediate size increase, but prevented large-scale aggregation ( d ). SDS-PAGE analysis confirmed the adsorption of serum proteins to both PS-COOH and PS-PEG nanoparticles after 1 h incubation in 10 % serum containing MEM (e) and verified reduced protein adsorption of PEG-coated nanoparticles after 24 h incubation ( f )
    Dmem Cell Culture Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem cell culture medium/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem cell culture medium - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Millipore dulbecco s modified eagle s medium ham s nutrient mixture f12
    Signaling assays. Aortic smooth muscle cells were isolated from Rgs5 +/+ and Rgs5 −/− mice and cultured in <t>Dulbecco's</t> modified <t>Eagle's</t> <t>medium-F12</t> containing 20% fetal calf serum. Cells were serum starved for 24 h
    Dulbecco S Modified Eagle S Medium Ham S Nutrient Mixture F12, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium ham s nutrient mixture f12/product/Millipore
    Average 97 stars, based on 1 article reviews
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    98
    Millipore dmem cultured jpcs
    Quantification of MSCA-1 + cells under <t>DMEM-</t> and MC-XF culturing conditions by FACS analysis. Unseparated <t>JPCs</t> were plated in culture dishes in DMEM medium. For the first test runs, cells were maintained in DMEM medium whereas the second series underwent stepwise FCS reduction and convertion to MC-XF medium. At the same time points (day 2, 5 and 9 after conversion from DMEM to MC-XF culture conditions), cells were detached from the dishes and the percentages of MSCA-1 + cells were determined by FACS analysis. Significant higher amounts of MSCA-1 + cells were detected under MC-XF culturing conditions at day 2 and 5 (p
    Dmem Cultured Jpcs, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem cultured jpcs/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem cultured jpcs - by Bioz Stars, 2021-03
    98/100 stars
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    97
    Millipore serum free chondrogenic induction medium
    <t>Chondrogenic</t> potential of human IPFSCs after FN1-KO. Human IPFSCs were chondrogenically induced in a pellet culture system for 18 days. The effect of fibronectin on chondrogenic capacity of human IPFSCs was evaluated using gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs and immunohistochemical staining (IHC) for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P
    Serum Free Chondrogenic Induction Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum free chondrogenic induction medium/product/Millipore
    Average 97 stars, based on 1 article reviews
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    Image Search Results


    Physical-chemical characterization of polystyrene NPs. Intensity weighted size distribution of carboxylated ( a ) and PEGylated ( b ) polystyrene nanoparticles measured by dynamic light scattering. Representative TEM images of the particles are shown in the top right panels of each DLS plot, scale bars represent 400 nm. c , d Aggregation–agglomeration properties and corona thickening of polystyrene nanoparticles was measured by DLS as a change in hydrodynamic size. Particles were incubated in inorganic ( c ) or biological solutions ( d ) for 96 h, and the size distribution was monitored. Color codes of samples are shown in the figure. Data are presented as mean ± standard deviation (n = 3). Particles showed no aggregation in distilled water or in PBS during the 96-h assay period ( c ). In contrast a time-dependent, heavy particle aggregation was found in serum-free DMEM (d). Incubation of nanoparticles with 10 % FBS also evoked an immediate size increase, but prevented large-scale aggregation ( d ). SDS-PAGE analysis confirmed the adsorption of serum proteins to both PS-COOH and PS-PEG nanoparticles after 1 h incubation in 10 % serum containing MEM (e) and verified reduced protein adsorption of PEG-coated nanoparticles after 24 h incubation ( f )

    Journal: Journal of Nanobiotechnology

    Article Title: Enhanced detection with spectral imaging fluorescence microscopy reveals tissue- and cell-type-specific compartmentalization of surface-modified polystyrene nanoparticles

    doi: 10.1186/s12951-016-0210-0

    Figure Lengend Snippet: Physical-chemical characterization of polystyrene NPs. Intensity weighted size distribution of carboxylated ( a ) and PEGylated ( b ) polystyrene nanoparticles measured by dynamic light scattering. Representative TEM images of the particles are shown in the top right panels of each DLS plot, scale bars represent 400 nm. c , d Aggregation–agglomeration properties and corona thickening of polystyrene nanoparticles was measured by DLS as a change in hydrodynamic size. Particles were incubated in inorganic ( c ) or biological solutions ( d ) for 96 h, and the size distribution was monitored. Color codes of samples are shown in the figure. Data are presented as mean ± standard deviation (n = 3). Particles showed no aggregation in distilled water or in PBS during the 96-h assay period ( c ). In contrast a time-dependent, heavy particle aggregation was found in serum-free DMEM (d). Incubation of nanoparticles with 10 % FBS also evoked an immediate size increase, but prevented large-scale aggregation ( d ). SDS-PAGE analysis confirmed the adsorption of serum proteins to both PS-COOH and PS-PEG nanoparticles after 1 h incubation in 10 % serum containing MEM (e) and verified reduced protein adsorption of PEG-coated nanoparticles after 24 h incubation ( f )

    Article Snippet: The suspensions were further diluted 1:10 with PBS (pH 7.4), DMEM cell culture medium (Sigma-Aldrich, St. Louis, MO, USA) or DMEM supplemented with 10 % fetal bovine serum (Invitrogen/Gibco, Carlsbad, CA, USA).

    Techniques: Transmission Electron Microscopy, Incubation, Standard Deviation, SDS Page, Adsorption

    Signaling assays. Aortic smooth muscle cells were isolated from Rgs5 +/+ and Rgs5 −/− mice and cultured in Dulbecco's modified Eagle's medium-F12 containing 20% fetal calf serum. Cells were serum starved for 24 h

    Journal:

    Article Title: Rgs5 Targeting Leads to Chronic Low Blood Pressure and a Lean Body Habitus ▿

    doi: 10.1128/MCB.01889-07

    Figure Lengend Snippet: Signaling assays. Aortic smooth muscle cells were isolated from Rgs5 +/+ and Rgs5 −/− mice and cultured in Dulbecco's modified Eagle's medium-F12 containing 20% fetal calf serum. Cells were serum starved for 24 h

    Article Snippet: Cells in culture were maintained in Dulbecco's modified Eagle's medium-F12 containing 20% fetal calf serum and 2 mM glutamine with penicillin and streptomycin and were serum starved for 24 h prior to the signaling assays.

    Techniques: Isolation, Mouse Assay, Cell Culture, Modification

    Quantification of MSCA-1 + cells under DMEM- and MC-XF culturing conditions by FACS analysis. Unseparated JPCs were plated in culture dishes in DMEM medium. For the first test runs, cells were maintained in DMEM medium whereas the second series underwent stepwise FCS reduction and convertion to MC-XF medium. At the same time points (day 2, 5 and 9 after conversion from DMEM to MC-XF culture conditions), cells were detached from the dishes and the percentages of MSCA-1 + cells were determined by FACS analysis. Significant higher amounts of MSCA-1 + cells were detected under MC-XF culturing conditions at day 2 and 5 (p

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Quantification of MSCA-1 + cells under DMEM- and MC-XF culturing conditions by FACS analysis. Unseparated JPCs were plated in culture dishes in DMEM medium. For the first test runs, cells were maintained in DMEM medium whereas the second series underwent stepwise FCS reduction and convertion to MC-XF medium. At the same time points (day 2, 5 and 9 after conversion from DMEM to MC-XF culture conditions), cells were detached from the dishes and the percentages of MSCA-1 + cells were determined by FACS analysis. Significant higher amounts of MSCA-1 + cells were detected under MC-XF culturing conditions at day 2 and 5 (p

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: FACS

    Detection of mineral deposition by mineralizing JPCs (of passage 6). The upper panel of the figure illustrates the beginning of mineralization at day 12 of osteogenic induction (ob) under both media conditions. Note that precipitate formation originates from only a few cells of the DMEM-cultured monolayer. In contrast, the MC-XF-cultivated monolayer seemed to be purer due to the appearance of mineralization potential originating from almost every cell of the monolayer. 4× magnification. The lower panel of the figure illustrates representative fluorescent stainings of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. JPCs cultivated under DMEM media conditions showed a stronger mineralization potential. 10× magnification.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Detection of mineral deposition by mineralizing JPCs (of passage 6). The upper panel of the figure illustrates the beginning of mineralization at day 12 of osteogenic induction (ob) under both media conditions. Note that precipitate formation originates from only a few cells of the DMEM-cultured monolayer. In contrast, the MC-XF-cultivated monolayer seemed to be purer due to the appearance of mineralization potential originating from almost every cell of the monolayer. 4× magnification. The lower panel of the figure illustrates representative fluorescent stainings of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. JPCs cultivated under DMEM media conditions showed a stronger mineralization potential. 10× magnification.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Cell Culture

    Expression patterns of DMEM- and MC-XF-cultured JPCs (growing within coated flasks) by flow cytometric analyses. Representative histograms and the average percentages (±STD) of positive cells for CD29, CD45, CD73, CD90 and CD105 expression by unseparated JPCs are illustrated.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Expression patterns of DMEM- and MC-XF-cultured JPCs (growing within coated flasks) by flow cytometric analyses. Representative histograms and the average percentages (±STD) of positive cells for CD29, CD45, CD73, CD90 and CD105 expression by unseparated JPCs are illustrated.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Expressing, Cell Culture, Flow Cytometry

    Detection of mineral deposition by non-mineralizing JPCs (of passage 6). Representative fluorescent staining of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. Non-mineralizing JPCs showed mineralization capacity only under MC-XF but not DMEM media conditions. 10× magnification. Co = untreated cell, ob = osteogenic induced cells.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Detection of mineral deposition by non-mineralizing JPCs (of passage 6). Representative fluorescent staining of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. Non-mineralizing JPCs showed mineralization capacity only under MC-XF but not DMEM media conditions. 10× magnification. Co = untreated cell, ob = osteogenic induced cells.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Staining, Cell Culture

    Quantitative analysis of gene expression levels in DMEM- and MC-XF-cultured JPCs at day 5 and 10 of osteogenesis (of passage 6, n = 4). Induction indices (x-fold) and significance values of alkaline phosphatase, Runx-2, type I collagen (alpha1-chain) and osteoprotegerin in osteogenic induced in comparison to untreated cells under both culture conditions are illustrated.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Quantitative analysis of gene expression levels in DMEM- and MC-XF-cultured JPCs at day 5 and 10 of osteogenesis (of passage 6, n = 4). Induction indices (x-fold) and significance values of alkaline phosphatase, Runx-2, type I collagen (alpha1-chain) and osteoprotegerin in osteogenic induced in comparison to untreated cells under both culture conditions are illustrated.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Expressing, Cell Culture

    Life-monitoring measurements of cell proliferation by unseparated JPCs using the x-CELLigence system (ACEA Biosciences). JPCs of passage 4 derived from two different donors were seeded into special E-plates in DMEM/F12/10%FCS culture medium. Two days later (44 hours - each tick of the scale corresponds to 11 hours), a gradual FCS reduction was performed in one-half of the test runs (green and dark green), whereas the other half of the wells was further cultivated in DMEM/F12/10%FCS (red and coral). Nine days (297 hours) after the initiation of FCS reduction, MC-XF culture medium was added to the cells. The proliferation curve progression of DMEM-cultured JPCs (from two representative patients) is highlighted in red and coral and that of MC-XF-cultivated cells is highlighted in dark green and green. The right panel of the figure shows the cell morphology of DMEM- and MC-XF-cultured JPCs. Note the reduced cell size under MC-XF culture conditions (on uncoated dishes) leading to the significant decrease of cell impedance immediately after the addition of the MC-XF culture medium.

    Journal: PLoS ONE

    Article Title: Selection of Osteoprogenitors from the Jaw Periosteum by a Specific Animal-Free Culture Medium

    doi: 10.1371/journal.pone.0081674

    Figure Lengend Snippet: Life-monitoring measurements of cell proliferation by unseparated JPCs using the x-CELLigence system (ACEA Biosciences). JPCs of passage 4 derived from two different donors were seeded into special E-plates in DMEM/F12/10%FCS culture medium. Two days later (44 hours - each tick of the scale corresponds to 11 hours), a gradual FCS reduction was performed in one-half of the test runs (green and dark green), whereas the other half of the wells was further cultivated in DMEM/F12/10%FCS (red and coral). Nine days (297 hours) after the initiation of FCS reduction, MC-XF culture medium was added to the cells. The proliferation curve progression of DMEM-cultured JPCs (from two representative patients) is highlighted in red and coral and that of MC-XF-cultivated cells is highlighted in dark green and green. The right panel of the figure shows the cell morphology of DMEM- and MC-XF-cultured JPCs. Note the reduced cell size under MC-XF culture conditions (on uncoated dishes) leading to the significant decrease of cell impedance immediately after the addition of the MC-XF culture medium.

    Article Snippet: Differentiation experiments DMEM-cultured JPCs (4×104 cells per well in 6-well plates) were treated with osteogenic medium (ob - DMEM/F12 containing 10% FCS, 10 mM β-glycerophosphate, 100 µM L-ascorbic acid 2-phosphate and 4 µm dexamethasone, Sigma-Aldrich) for 30 days.

    Techniques: Derivative Assay, Cell Culture

    Chondrogenic potential of human IPFSCs after FN1-KO. Human IPFSCs were chondrogenically induced in a pellet culture system for 18 days. The effect of fibronectin on chondrogenic capacity of human IPFSCs was evaluated using gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs and immunohistochemical staining (IHC) for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

    doi: 10.3389/fbioe.2019.00321

    Figure Lengend Snippet: Chondrogenic potential of human IPFSCs after FN1-KO. Human IPFSCs were chondrogenically induced in a pellet culture system for 18 days. The effect of fibronectin on chondrogenic capacity of human IPFSCs was evaluated using gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs and immunohistochemical staining (IHC) for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Article Snippet: After overnight incubation (day 0), pellets were grown in a serum-free chondrogenic induction medium [high-glucose Dulbecco's modified Eagle's medium (DMEM) with 40 μg/ml proline (MilliporeSigma), 100 nM dexamethasone (MilliporeSigma), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM ascorbic acid-2-phosphate, and 1 × ITS™ Premix (BD Biosciences)] with the supplementation of 10 ng/ml transforming growth factor beta3 (TGFβ3; PeproTech, Rocky Hill, NJ) for up to 18 days.

    Techniques: Staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Marker

    Chondrogenic potential of human IPFSCs after expansion on dECMs deposited by FN1-KO cells. Passage 15 human IPFSCs in a pellet culture system were compared for chondrogenic capacity after expansion on dECMs deposited by Cas9-sgFN1a/b transduced cells (sgFN1a ECM and sgFN1b ECM, respectively) with those deposited by copGFP (copGFP ECM) and those grown on TCP (TCP) as controls including gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs, and IHC for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

    doi: 10.3389/fbioe.2019.00321

    Figure Lengend Snippet: Chondrogenic potential of human IPFSCs after expansion on dECMs deposited by FN1-KO cells. Passage 15 human IPFSCs in a pellet culture system were compared for chondrogenic capacity after expansion on dECMs deposited by Cas9-sgFN1a/b transduced cells (sgFN1a ECM and sgFN1b ECM, respectively) with those deposited by copGFP (copGFP ECM) and those grown on TCP (TCP) as controls including gross observation of 18-day pellets, Alcian blue staining (Ab) for sulfated GAGs, and IHC for type II collagen (Col2) (A) . qPCR was used to evaluate expression of chondrogenic marker genes ( SOX9, ACAN, COL2A1 , and PRG4 ) and hypertrophic marker genes ( COL10A1 and MMP13 ) (B) . GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P

    Article Snippet: After overnight incubation (day 0), pellets were grown in a serum-free chondrogenic induction medium [high-glucose Dulbecco's modified Eagle's medium (DMEM) with 40 μg/ml proline (MilliporeSigma), 100 nM dexamethasone (MilliporeSigma), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 mM ascorbic acid-2-phosphate, and 1 × ITS™ Premix (BD Biosciences)] with the supplementation of 10 ng/ml transforming growth factor beta3 (TGFβ3; PeproTech, Rocky Hill, NJ) for up to 18 days.

    Techniques: Staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Marker