dual pump dionex ultimate 3000 rapid separation ultra high performance liquid chromatography uhplc  (Thermo Fisher)


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    Thermo Fisher dual pump dionex ultimate 3000 rapid separation ultra high performance liquid chromatography uhplc
    Dual Pump Dionex Ultimate 3000 Rapid Separation Ultra High Performance Liquid Chromatography Uhplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual pump dionex ultimate 3000 rapid separation ultra high performance liquid chromatography uhplc/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dual pump dionex ultimate 3000 rapid separation ultra high performance liquid chromatography uhplc - by Bioz Stars, 2020-09
    92/100 stars

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    Liquid Chromatography:

    Article Title: The Pseudomonas aeruginosa PrrF1 and PrrF2 Small Regulatory RNAs Promote 2-Alkyl-4-Quinolone Production through Redundant Regulation of the antR mRNA
    Article Snippet: .. The AQ extracts were resuspended in a mobile phase of 1:1 acetonitrile-water with 0.1% formic acid and 200 μM EDTA and separated using a dual-pump Dionex Ultimate 3000 Rapid Separation ultra-high performance liquid chromatography (UHPLC) device (Thermo-Fisher). .. Separation was attained using an Ascentis Express C8 column (100 by 2.1 mm; 2.7-μm particle size; Sigma-Aldrich) maintained at 30°C.

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    Thermo Fisher ultimate 3000 uhplc instrument
    Chemoselective synthesis of biotinylated R7A Nod factor and chitin pentamer conjugates. ( A ) Synthesis of biotinylated Mesorhizobium loti , strain R7A Nod factor conjugate. Conditions: (i) R7A Nod factor (5 mM), O -(2-aminoethyl)- N -methyl hydroxylamine (150 mM), 0.62 M acetate buffer at pH 4.5 containing 50% acetonitrile, 16 hr; (ii) N -hydroxysuccinimidyl–dPEG4–biotin (15 mM), 50 mM borate buffer at pH 8.5 containing 50% acetonitrile, 16 hr. ( B ) Structure of analogously synthesized biotinylated chitopentaose conjugate. ( C ) High-performance liquid chromatography (HPLC) chromatogram (215 nm) and high-resolution mass spectrometric (HR-MS) data for biotinylated R7A Nod factor conjugate. The chromatogram displays overlapping peaks, owing to a distribution of OAc groups on the fucosyl unit (main species include 3- O -acetylated, 4- O -acetylated, or non-acetylated fucosyl unit). High-resolution mass spectra (HR-MS) were obtained using a Dionex <t>Ultimate</t> 3000 <t>UHPLC</t> instrument (Thermo) coupled to a Bruker Impact HDII QTOF mass spectrometer. Mass spectra shown for [M + 2 hr]2 + for 3- O and 4- O -acetylated species (upper panel) and non-acetylated species (lower panel). ( D ), HPLC chromatogram (215 nm) and HR-MS data for biotinylated chitopentaose conjugate. Mass spectrum shown for [M + 2 hr]2+.
    Ultimate 3000 Uhplc Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate 3000 uhplc instrument/product/Thermo Fisher
    Average 99 stars, based on 170 article reviews
    Price from $9.99 to $1999.99
    ultimate 3000 uhplc instrument - by Bioz Stars, 2020-09
    99/100 stars
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    Chemoselective synthesis of biotinylated R7A Nod factor and chitin pentamer conjugates. ( A ) Synthesis of biotinylated Mesorhizobium loti , strain R7A Nod factor conjugate. Conditions: (i) R7A Nod factor (5 mM), O -(2-aminoethyl)- N -methyl hydroxylamine (150 mM), 0.62 M acetate buffer at pH 4.5 containing 50% acetonitrile, 16 hr; (ii) N -hydroxysuccinimidyl–dPEG4–biotin (15 mM), 50 mM borate buffer at pH 8.5 containing 50% acetonitrile, 16 hr. ( B ) Structure of analogously synthesized biotinylated chitopentaose conjugate. ( C ) High-performance liquid chromatography (HPLC) chromatogram (215 nm) and high-resolution mass spectrometric (HR-MS) data for biotinylated R7A Nod factor conjugate. The chromatogram displays overlapping peaks, owing to a distribution of OAc groups on the fucosyl unit (main species include 3- O -acetylated, 4- O -acetylated, or non-acetylated fucosyl unit). High-resolution mass spectra (HR-MS) were obtained using a Dionex Ultimate 3000 UHPLC instrument (Thermo) coupled to a Bruker Impact HDII QTOF mass spectrometer. Mass spectra shown for [M + 2 hr]2 + for 3- O and 4- O -acetylated species (upper panel) and non-acetylated species (lower panel). ( D ), HPLC chromatogram (215 nm) and HR-MS data for biotinylated chitopentaose conjugate. Mass spectrum shown for [M + 2 hr]2+.

    Journal: eLife

    Article Title: Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus

    doi: 10.7554/eLife.33506

    Figure Lengend Snippet: Chemoselective synthesis of biotinylated R7A Nod factor and chitin pentamer conjugates. ( A ) Synthesis of biotinylated Mesorhizobium loti , strain R7A Nod factor conjugate. Conditions: (i) R7A Nod factor (5 mM), O -(2-aminoethyl)- N -methyl hydroxylamine (150 mM), 0.62 M acetate buffer at pH 4.5 containing 50% acetonitrile, 16 hr; (ii) N -hydroxysuccinimidyl–dPEG4–biotin (15 mM), 50 mM borate buffer at pH 8.5 containing 50% acetonitrile, 16 hr. ( B ) Structure of analogously synthesized biotinylated chitopentaose conjugate. ( C ) High-performance liquid chromatography (HPLC) chromatogram (215 nm) and high-resolution mass spectrometric (HR-MS) data for biotinylated R7A Nod factor conjugate. The chromatogram displays overlapping peaks, owing to a distribution of OAc groups on the fucosyl unit (main species include 3- O -acetylated, 4- O -acetylated, or non-acetylated fucosyl unit). High-resolution mass spectra (HR-MS) were obtained using a Dionex Ultimate 3000 UHPLC instrument (Thermo) coupled to a Bruker Impact HDII QTOF mass spectrometer. Mass spectra shown for [M + 2 hr]2 + for 3- O and 4- O -acetylated species (upper panel) and non-acetylated species (lower panel). ( D ), HPLC chromatogram (215 nm) and HR-MS data for biotinylated chitopentaose conjugate. Mass spectrum shown for [M + 2 hr]2+.

    Article Snippet: High-resolution mass spectra (HR-MS) were obtained using a Dionex Ultimate 3000 UHPLC instrument (Thermo) coupled to a Bruker Impact HDII QTOF mass spectrometer.

    Techniques: Synthesized, High Performance Liquid Chromatography, Mass Spectrometry