du145 htb 81 cells  (ATCC)


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    ATCC du145 htb 81 cells
    Du145 Htb 81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    du145 htb 81 cells - by Bioz Stars, 2024-09
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    du145 htb 81 cells  (ATCC)


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    ATCC du145 htb 81 cells
    ROR1 levels are elevated in aggressive prostate cancer cell lines. ( A ) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 in RWPE-1 (normal prostate epithelium), LNCAP (AR pos -AD), <t>DU145</t> (AR neg -AI), and PC3 (AR neg -AI). ( B ) Quantification of immunoblot bands pROR1/ROR1 across different cell lines (all normalized to HSP40). Data are expressed as the mean +/− SEM from three independent western blots. ( C ) Quantification of immunoblot band ROR1 across different cell lines (all normalized to HSP40). Data are expressed as the mean +/− SEM from three independent western blots. [N = 3. ** = p value < 0.01; * = p value < 0.05].
    Du145 Htb 81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    du145 htb 81 cells - by Bioz Stars, 2024-09
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    1) Product Images from "Pentagalloyl Glucose (PGG) Exhibits Anti-Cancer Activity against Aggressive Prostate Cancer by Modulating the ROR1 Mediated AKT-GSK3β Pathway"

    Article Title: Pentagalloyl Glucose (PGG) Exhibits Anti-Cancer Activity against Aggressive Prostate Cancer by Modulating the ROR1 Mediated AKT-GSK3β Pathway

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25137003

    ROR1 levels are elevated in aggressive prostate cancer cell lines. ( A ) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 in RWPE-1 (normal prostate epithelium), LNCAP (AR pos -AD), DU145 (AR neg -AI), and PC3 (AR neg -AI). ( B ) Quantification of immunoblot bands pROR1/ROR1 across different cell lines (all normalized to HSP40). Data are expressed as the mean +/− SEM from three independent western blots. ( C ) Quantification of immunoblot band ROR1 across different cell lines (all normalized to HSP40). Data are expressed as the mean +/− SEM from three independent western blots. [N = 3. ** = p value < 0.01; * = p value < 0.05].
    Figure Legend Snippet: ROR1 levels are elevated in aggressive prostate cancer cell lines. ( A ) Representative western blot of phospho-ROR1 (Tyr786) and ROR1 in RWPE-1 (normal prostate epithelium), LNCAP (AR pos -AD), DU145 (AR neg -AI), and PC3 (AR neg -AI). ( B ) Quantification of immunoblot bands pROR1/ROR1 across different cell lines (all normalized to HSP40). Data are expressed as the mean +/− SEM from three independent western blots. ( C ) Quantification of immunoblot band ROR1 across different cell lines (all normalized to HSP40). Data are expressed as the mean +/− SEM from three independent western blots. [N = 3. ** = p value < 0.01; * = p value < 0.05].

    Techniques Used: Western Blot

    PGG exhibits anti-migratory and anti-invasive effects on DU145 cells. ( A ) Wound healing assay to assess the migration of DU145 cells after treatment with vehicle, 7 μM PGG, or 15 μM PGG for 24 h. ( B ) Quantification of the % wound healed to assess the migration of DU145 cells after 24-h vehicle or PGG treatment. ( C ) Boyden chamber assay to assess the invasion of DU145 cells through a basement membrane after treatment with vehicle or 31.25 μM PGG for 24 h. ( D ) Quantification of the % area covered by crystal violet stained cells to assess the invasion of DU145 cells after 24-h vehicle or PGG treatment. ( E ) Representative data of Muse analyzer flow cytometry apoptosis profile of DU145 cells treated with vehicle or 31.25 μM PGG for 48 h. Cells were sorted into live, apoptotic, apoptotic/dead, or dead categories by intensity of caspase 3 and 7 expression. ( F ) Quantification of flow cytometry apoptosis profile on treated DU145 cells. [N ≥ 3. * = p value < 0.05; ns = not significant].
    Figure Legend Snippet: PGG exhibits anti-migratory and anti-invasive effects on DU145 cells. ( A ) Wound healing assay to assess the migration of DU145 cells after treatment with vehicle, 7 μM PGG, or 15 μM PGG for 24 h. ( B ) Quantification of the % wound healed to assess the migration of DU145 cells after 24-h vehicle or PGG treatment. ( C ) Boyden chamber assay to assess the invasion of DU145 cells through a basement membrane after treatment with vehicle or 31.25 μM PGG for 24 h. ( D ) Quantification of the % area covered by crystal violet stained cells to assess the invasion of DU145 cells after 24-h vehicle or PGG treatment. ( E ) Representative data of Muse analyzer flow cytometry apoptosis profile of DU145 cells treated with vehicle or 31.25 μM PGG for 48 h. Cells were sorted into live, apoptotic, apoptotic/dead, or dead categories by intensity of caspase 3 and 7 expression. ( F ) Quantification of flow cytometry apoptosis profile on treated DU145 cells. [N ≥ 3. * = p value < 0.05; ns = not significant].

    Techniques Used: Wound Healing Assay, Migration, Boyden Chamber Assay, Membrane, Staining, Flow Cytometry, Expressing

    du145 htb 81 cell lines  (ATCC)


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    ATCC du145 htb 81 cell lines
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du145 htb 81 cell lines/product/ATCC
    Average 86 stars, based on 1 article reviews
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    du145 htb 81 cell lines  (ATCC)


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    ATCC du145 htb 81 cell lines
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du145 htb 81 cell lines/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    du145 htb 81 cell lines  (ATCC)


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    ATCC du145 htb 81 cell lines
    A, Graphical scheme of the experimental design of CRISPR/Cas9 screenings . B, Volcano plot showing PC3 CRISPR/Cas9 screening results. C, Volcano plot showing <t>DU145</t> CRISPR/Cas9 screening results. D, Venn diagram showing the number of genes significantly associated with PCa invasive process in each line and the number of common genes between both screenings.
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du145 htb 81 cell lines/product/ATCC
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    du145 htb 81 cell lines - by Bioz Stars, 2024-09
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    1) Product Images from "CRISPR/Cas9 screenings unearth protein arginine methyltransferase 7 as a novel driver of metastasis in prostate cancer"

    Article Title: CRISPR/Cas9 screenings unearth protein arginine methyltransferase 7 as a novel driver of metastasis in prostate cancer

    Journal: bioRxiv

    doi: 10.1101/2023.07.20.549704

    A, Graphical scheme of the experimental design of CRISPR/Cas9 screenings . B, Volcano plot showing PC3 CRISPR/Cas9 screening results. C, Volcano plot showing DU145 CRISPR/Cas9 screening results. D, Venn diagram showing the number of genes significantly associated with PCa invasive process in each line and the number of common genes between both screenings.
    Figure Legend Snippet: A, Graphical scheme of the experimental design of CRISPR/Cas9 screenings . B, Volcano plot showing PC3 CRISPR/Cas9 screening results. C, Volcano plot showing DU145 CRISPR/Cas9 screening results. D, Venn diagram showing the number of genes significantly associated with PCa invasive process in each line and the number of common genes between both screenings.

    Techniques Used: CRISPR

    Gene ontology biological pathway enrichment analysis (GO:BP) using Metascape of (A) PC3 and (B) DU145 results. C-D, Biological pathways GSEA in (C) PC3 and (D) DU145 screening results.
    Figure Legend Snippet: Gene ontology biological pathway enrichment analysis (GO:BP) using Metascape of (A) PC3 and (B) DU145 results. C-D, Biological pathways GSEA in (C) PC3 and (D) DU145 screening results.

    Techniques Used:

    A, Invasion assay of PC3 inhibited cells using specific siRNA to target our best gene candidates versus control (siCTL) cells. B-C, Representative western blot of PRMT7, PRMT5 and β-actin protein levels in (B) PC3-Cas9 and (C) DU145-Cas9 cell lines. The numbers below each lane represent PRMT7/β-actin or PRMT5/β-actin respectively densitometric quantification is referred to control cells. (n=3). D-E, Invasion assay of PRMT7 depleted versus control (CTL) cells of (D) PC3-Cas9 and (E) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001). F-G, Migration assay of PRMT7 depleted versus CTL cells of (F) PC3-Cas9 and (G) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). H-I, Viability assay of PRMT7 depleted versus CTL cells of (H) PC3- Cas9 and (I) DU145-Cas9 cells (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Figure Legend Snippet: A, Invasion assay of PC3 inhibited cells using specific siRNA to target our best gene candidates versus control (siCTL) cells. B-C, Representative western blot of PRMT7, PRMT5 and β-actin protein levels in (B) PC3-Cas9 and (C) DU145-Cas9 cell lines. The numbers below each lane represent PRMT7/β-actin or PRMT5/β-actin respectively densitometric quantification is referred to control cells. (n=3). D-E, Invasion assay of PRMT7 depleted versus control (CTL) cells of (D) PC3-Cas9 and (E) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001). F-G, Migration assay of PRMT7 depleted versus CTL cells of (F) PC3-Cas9 and (G) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). H-I, Viability assay of PRMT7 depleted versus CTL cells of (H) PC3- Cas9 and (I) DU145-Cas9 cells (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Techniques Used: Invasion Assay, Western Blot, Migration, Viability Assay

    A, Volcano plot showing differentially expressed genes (DEGs) (adjusted p-value < 0.05, |logFC| > 1) from the differential gene expression analysis of PC3 control versus PRMT7 depleted cells results, and a barplot representing the number of genes that significantly changed their expression, accounting for the number of genes that were upregulated (red) and downregulated (blue) in PRMT7 depleted cells. B, Over-representation analysis of biological process GO terms in DEGs (adjusted p-value < 0.05, |logFC| > 1) of PRMT7 depleted cells compared to control cells, representing top 25 terms with highest gene ratio with an adjusted p-value cutoff of 0.05 and redundant GO terms were removed. C, Heatmap showing the gene expression (Z-score) of the genes found in parental GO term cell adhesion sorted by log 2 fold change. Horizontal lines denote DEG positions, and those of interest are highlighted in red. D-E, Western blot analysis of ITGα1 and ITGβ4 protein levels in (D) PC3-Cas9 and (E) DU145-Cas9 cells. The numbers below each lane represent ITGα1/β-actin or ITGβ4/β-actin densitometric quantification referred to control cells, respectively. F-G, Graph representing mean number of cells per field adhered to (F) collagen IV or (G) laminin (mean ± SEM of n=3, by unpaired Student’s t test *P<0.05, **P>0.01).
    Figure Legend Snippet: A, Volcano plot showing differentially expressed genes (DEGs) (adjusted p-value < 0.05, |logFC| > 1) from the differential gene expression analysis of PC3 control versus PRMT7 depleted cells results, and a barplot representing the number of genes that significantly changed their expression, accounting for the number of genes that were upregulated (red) and downregulated (blue) in PRMT7 depleted cells. B, Over-representation analysis of biological process GO terms in DEGs (adjusted p-value < 0.05, |logFC| > 1) of PRMT7 depleted cells compared to control cells, representing top 25 terms with highest gene ratio with an adjusted p-value cutoff of 0.05 and redundant GO terms were removed. C, Heatmap showing the gene expression (Z-score) of the genes found in parental GO term cell adhesion sorted by log 2 fold change. Horizontal lines denote DEG positions, and those of interest are highlighted in red. D-E, Western blot analysis of ITGα1 and ITGβ4 protein levels in (D) PC3-Cas9 and (E) DU145-Cas9 cells. The numbers below each lane represent ITGα1/β-actin or ITGβ4/β-actin densitometric quantification referred to control cells, respectively. F-G, Graph representing mean number of cells per field adhered to (F) collagen IV or (G) laminin (mean ± SEM of n=3, by unpaired Student’s t test *P<0.05, **P>0.01).

    Techniques Used: Expressing, Western Blot

    A Hematoxylin and eosin staining of PCa primary tumor slides showing the aggressiveness of tumors measured by Gleason score PRMT7 (scale bars: 50 μM). B, PRMT7 gene expression levels in PCa primary tumor samples with higher (n=8) versus lower (n=5) Gleason score samples (mean ± SEM of n=13 primary tumor samples, by unpaired Mann-Whitney test *p < 0.05, **p < 0.01, ***p < 0.001). C, D, Representative western blot of arginine monomethylation (MMA) levels in (C) PC3 and (D) DU145 cells treated with 10μM of SGC3027 (PRMT7 inhibitor). The numbers below each lane represent MMA/β-actin densitometric quantification referred to control cells. E-F, Invasion assay of PRMT7 inhibited versus vehicle cells of (E) PC3 and (F) DU145 cells (mean ± SEM of n=6 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). G-H, Cell viability assay of PRMT7 inhibited versus vehicle cells of (G) PC3 and (H) DU145 cell lines (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Figure Legend Snippet: A Hematoxylin and eosin staining of PCa primary tumor slides showing the aggressiveness of tumors measured by Gleason score PRMT7 (scale bars: 50 μM). B, PRMT7 gene expression levels in PCa primary tumor samples with higher (n=8) versus lower (n=5) Gleason score samples (mean ± SEM of n=13 primary tumor samples, by unpaired Mann-Whitney test *p < 0.05, **p < 0.01, ***p < 0.001). C, D, Representative western blot of arginine monomethylation (MMA) levels in (C) PC3 and (D) DU145 cells treated with 10μM of SGC3027 (PRMT7 inhibitor). The numbers below each lane represent MMA/β-actin densitometric quantification referred to control cells. E-F, Invasion assay of PRMT7 inhibited versus vehicle cells of (E) PC3 and (F) DU145 cells (mean ± SEM of n=6 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). G-H, Cell viability assay of PRMT7 inhibited versus vehicle cells of (G) PC3 and (H) DU145 cell lines (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Techniques Used: Staining, Expressing, MANN-WHITNEY, Western Blot, Invasion Assay, Viability Assay

    prostate cancer pca cell lines du145 atcc htb 81  (ATCC)


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    ATCC prostate cancer pca cell lines du145 atcc htb 81
    Advanced <t>prostate</t> <t>cancer</t> <t>cells</t> secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and <t>DU145</t> supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Prostate Cancer Pca Cell Lines Du145 Atcc Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells"

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    Journal: Journal of Immunology Research

    doi: 10.1155/2022/1810804

    Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Figure Legend Snippet: Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Techniques Used: Concentration Assay, Cell Culture

    The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Figure Legend Snippet: The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Techniques Used: Expressing, Cell Culture, Flow Cytometry

    Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.
    Figure Legend Snippet: Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.

    Techniques Used: De-Phosphorylation Assay, Cell Culture, Expressing, Western Blot, In-Cell ELISA, Activity Assay, Concentration Assay

    Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.
    Figure Legend Snippet: Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.

    Techniques Used:

    cell lines du145 htb 81  (ATCC)


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    ATCC cell lines du145 htb 81
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    cell lines du145 htb 81 - by Bioz Stars, 2024-09
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    1) Product Images from "Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines"

    Article Title: Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064426

    DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Figure Legend Snippet: DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.

    Techniques Used: Staining, Incubation, Flow Cytometry

    du145 htb 81 cells  (ATCC)


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    ATCC du145 htb 81 cells
    JICD promotes prostate cancer stem–like cell properties in prostate cancer cells. ( A , B ) Overexpression of JICD increases CSC marker expression in prostate cancer cells. Representative Western blot analysis ( A ) showing the protein levels of CD133, NANOG, BIM, BCL–XL, and JICD; Representative RT–PCR ( top ) and quantitative RT–PCR (qPCR; bottom ) analysis of three independent experiments ( B ) revealing the mRNA levels of CD133 and JICD in stable EV– or JICD–overexpressing CWR22Rv1 subline cells. GAPDH was used as a loading control. Data are shown as mean ± SEM, three independent experiments. *, p < 0.01; two–tailed t -test. ( C ) Heatmap showing the strong upregulation of CD63, ASNS, TEX264, and FOXM1 and the downregulation of RB1 and P53 downstream signaling pathways (RB1, TP53BP2, TP53INP1, and RRM2B), which are related to stem-like cell properties. ( D , E ) JICD overexpression facilitates and maintains sphere formation. Representative images of stable EV– or JICD–overexpressing CWR22Rv1 spheres grown for the sphere formation ( D , top ) and modified 3D cell culture ( E , top ) assays (magnification, ×10 and ×20; bars, 400 and 200 μm, respectively). Arrowheads ( D , E , top ) indicate the spheres with differentiated morphology. Graphs presenting the number of spheres versus the diameter of spheres (μm) derived from stable EV– or JICD–overexpressing CWR22Rv1 cells, which were grown under sphere formation ( D , bottom ) and modified 3D cell culture ( E , bottom ) conditions. Data are shown as mean ± SEM. *, p < 0.05; **, p < 0.01; ns, not significant; one-way ANOVA with Tukey’s post-hoc test. ( F ) Representative Western blot analysis showing that spheres derived from stable JICD–overexpressing CWR22Rv1 cells expressed higher protein levels of CD133, NANOG, OCT3/4, and ARs, especially AR–Vs, including AR–V7, than the control EV cell-derived spheres did. Values above Western blots indicate the relative band intensity of each protein normalized to GAPDH and are shown as mean ± SD, which were obtained by densitometrical quantification of at least three independent experiments. All original uncropped Western blot and gel images are shown in .
    Du145 Htb 81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "JAG1 Intracellular Domain Enhances AR Expression and Signaling and Promotes Stem-like Properties in Prostate Cancer Cells"

    Article Title: JAG1 Intracellular Domain Enhances AR Expression and Signaling and Promotes Stem-like Properties in Prostate Cancer Cells

    Journal: Cancers

    doi: 10.3390/cancers14225714

    JICD promotes prostate cancer stem–like cell properties in prostate cancer cells. ( A , B ) Overexpression of JICD increases CSC marker expression in prostate cancer cells. Representative Western blot analysis ( A ) showing the protein levels of CD133, NANOG, BIM, BCL–XL, and JICD; Representative RT–PCR ( top ) and quantitative RT–PCR (qPCR; bottom ) analysis of three independent experiments ( B ) revealing the mRNA levels of CD133 and JICD in stable EV– or JICD–overexpressing CWR22Rv1 subline cells. GAPDH was used as a loading control. Data are shown as mean ± SEM, three independent experiments. *, p < 0.01; two–tailed t -test. ( C ) Heatmap showing the strong upregulation of CD63, ASNS, TEX264, and FOXM1 and the downregulation of RB1 and P53 downstream signaling pathways (RB1, TP53BP2, TP53INP1, and RRM2B), which are related to stem-like cell properties. ( D , E ) JICD overexpression facilitates and maintains sphere formation. Representative images of stable EV– or JICD–overexpressing CWR22Rv1 spheres grown for the sphere formation ( D , top ) and modified 3D cell culture ( E , top ) assays (magnification, ×10 and ×20; bars, 400 and 200 μm, respectively). Arrowheads ( D , E , top ) indicate the spheres with differentiated morphology. Graphs presenting the number of spheres versus the diameter of spheres (μm) derived from stable EV– or JICD–overexpressing CWR22Rv1 cells, which were grown under sphere formation ( D , bottom ) and modified 3D cell culture ( E , bottom ) conditions. Data are shown as mean ± SEM. *, p < 0.05; **, p < 0.01; ns, not significant; one-way ANOVA with Tukey’s post-hoc test. ( F ) Representative Western blot analysis showing that spheres derived from stable JICD–overexpressing CWR22Rv1 cells expressed higher protein levels of CD133, NANOG, OCT3/4, and ARs, especially AR–Vs, including AR–V7, than the control EV cell-derived spheres did. Values above Western blots indicate the relative band intensity of each protein normalized to GAPDH and are shown as mean ± SD, which were obtained by densitometrical quantification of at least three independent experiments. All original uncropped Western blot and gel images are shown in .
    Figure Legend Snippet: JICD promotes prostate cancer stem–like cell properties in prostate cancer cells. ( A , B ) Overexpression of JICD increases CSC marker expression in prostate cancer cells. Representative Western blot analysis ( A ) showing the protein levels of CD133, NANOG, BIM, BCL–XL, and JICD; Representative RT–PCR ( top ) and quantitative RT–PCR (qPCR; bottom ) analysis of three independent experiments ( B ) revealing the mRNA levels of CD133 and JICD in stable EV– or JICD–overexpressing CWR22Rv1 subline cells. GAPDH was used as a loading control. Data are shown as mean ± SEM, three independent experiments. *, p < 0.01; two–tailed t -test. ( C ) Heatmap showing the strong upregulation of CD63, ASNS, TEX264, and FOXM1 and the downregulation of RB1 and P53 downstream signaling pathways (RB1, TP53BP2, TP53INP1, and RRM2B), which are related to stem-like cell properties. ( D , E ) JICD overexpression facilitates and maintains sphere formation. Representative images of stable EV– or JICD–overexpressing CWR22Rv1 spheres grown for the sphere formation ( D , top ) and modified 3D cell culture ( E , top ) assays (magnification, ×10 and ×20; bars, 400 and 200 μm, respectively). Arrowheads ( D , E , top ) indicate the spheres with differentiated morphology. Graphs presenting the number of spheres versus the diameter of spheres (μm) derived from stable EV– or JICD–overexpressing CWR22Rv1 cells, which were grown under sphere formation ( D , bottom ) and modified 3D cell culture ( E , bottom ) conditions. Data are shown as mean ± SEM. *, p < 0.05; **, p < 0.01; ns, not significant; one-way ANOVA with Tukey’s post-hoc test. ( F ) Representative Western blot analysis showing that spheres derived from stable JICD–overexpressing CWR22Rv1 cells expressed higher protein levels of CD133, NANOG, OCT3/4, and ARs, especially AR–Vs, including AR–V7, than the control EV cell-derived spheres did. Values above Western blots indicate the relative band intensity of each protein normalized to GAPDH and are shown as mean ± SD, which were obtained by densitometrical quantification of at least three independent experiments. All original uncropped Western blot and gel images are shown in .

    Techniques Used: Over Expression, Marker, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Two Tailed Test, Modification, Cell Culture, Derivative Assay

    JICD overexpression increases prostate cancer cell mobility and in vivo tumorigenesis. ( A , B ) Stable JICD overexpression reduces the proliferation and viability of CWR22Rv1 cells. Stable EV (#1) or JICD (#2) CWR22Rv1 subline cells were maintained for 3 days and then subjected to MTS/PMS assays ( A , left ) or cell counting assay ( A , right ). Stable EV (#1 and #3) or JICD (#2 and #4) subline cells were maintained for 1 and 6 days, stained with crystal violet, and imaged via ZEISS microscopy at 20× magnification (scale bar 10 μm) ( B ). Data are shown as mean ± SEM. *, p < 0.01; two-tailed t -test. ( C ) Colony size increases in stable JICD-overexpressing CWR22Rv1 subline cells compared with that in the control without significant difference in the total number of colonies between them. Stable EV (#1) or JICD (#2) subline cells were maintained for 2 weeks and then processed for 0.5% crystal violet staining. Colonies were imaged with a normal camera. ( D – F ) JICD increases the mobility of prostate cancer cells. Invasion assays of stable EV (#1) or JICD (#2) CWR22Rv1 subline cells ( D ). Cell invasion was allowed to occur for 48 h, stained with crystal violet, and imaged via ZEISS microscopy at 10× magnification (Scale bar = 10 μm). Boyden Chamber migration assay of stable EV– or JICD–overexpressing CWR22Rv1 cells ( E ). Cells were allowed to migrate for 24 h, stained with crystal violet, and imaged with ZEISS microscopy at 10× magnification (Scale bar = 10 μm). Scratch wound-closure assay of stable EV (#1) or JICD (#2) CWR22Rv1 subline cells ( F ). Scratch wounds were generated (day 0, D0), and wound distances were monitored for 2 (D2) and 4 (D4) days; they were then imaged using EVOS ® FL Cell Imaging System at 4× magnification (Scale bar = 1000 μm). Boundary lines were simply drawn using the shape tool from PowerPoint. ( G – I ) JICD overexpression promotes CWR22Rv1 xenograft tumor growth in vivo. Stable JICD–overexpressing (JICD; #2) or control (EV; #1) CWR22Rv1 subline cells were implanted into the shoulders of 4–week–old male NOD.CB17–PrkdcSCID/J mice, and tumors were allowed to grow for 7 weeks. Representative tumors ( G ) dissected from mice after 7 weeks. Tumor weight ( H ) and body weight ( I ) measured after 7 weeks. Data are shown as mean ± SEM, two independent biological in vivo experiments ( n = 6 mice per each group). *, p < 0.01; ns, not significant; two–tailed t -test.
    Figure Legend Snippet: JICD overexpression increases prostate cancer cell mobility and in vivo tumorigenesis. ( A , B ) Stable JICD overexpression reduces the proliferation and viability of CWR22Rv1 cells. Stable EV (#1) or JICD (#2) CWR22Rv1 subline cells were maintained for 3 days and then subjected to MTS/PMS assays ( A , left ) or cell counting assay ( A , right ). Stable EV (#1 and #3) or JICD (#2 and #4) subline cells were maintained for 1 and 6 days, stained with crystal violet, and imaged via ZEISS microscopy at 20× magnification (scale bar 10 μm) ( B ). Data are shown as mean ± SEM. *, p < 0.01; two-tailed t -test. ( C ) Colony size increases in stable JICD-overexpressing CWR22Rv1 subline cells compared with that in the control without significant difference in the total number of colonies between them. Stable EV (#1) or JICD (#2) subline cells were maintained for 2 weeks and then processed for 0.5% crystal violet staining. Colonies were imaged with a normal camera. ( D – F ) JICD increases the mobility of prostate cancer cells. Invasion assays of stable EV (#1) or JICD (#2) CWR22Rv1 subline cells ( D ). Cell invasion was allowed to occur for 48 h, stained with crystal violet, and imaged via ZEISS microscopy at 10× magnification (Scale bar = 10 μm). Boyden Chamber migration assay of stable EV– or JICD–overexpressing CWR22Rv1 cells ( E ). Cells were allowed to migrate for 24 h, stained with crystal violet, and imaged with ZEISS microscopy at 10× magnification (Scale bar = 10 μm). Scratch wound-closure assay of stable EV (#1) or JICD (#2) CWR22Rv1 subline cells ( F ). Scratch wounds were generated (day 0, D0), and wound distances were monitored for 2 (D2) and 4 (D4) days; they were then imaged using EVOS ® FL Cell Imaging System at 4× magnification (Scale bar = 1000 μm). Boundary lines were simply drawn using the shape tool from PowerPoint. ( G – I ) JICD overexpression promotes CWR22Rv1 xenograft tumor growth in vivo. Stable JICD–overexpressing (JICD; #2) or control (EV; #1) CWR22Rv1 subline cells were implanted into the shoulders of 4–week–old male NOD.CB17–PrkdcSCID/J mice, and tumors were allowed to grow for 7 weeks. Representative tumors ( G ) dissected from mice after 7 weeks. Tumor weight ( H ) and body weight ( I ) measured after 7 weeks. Data are shown as mean ± SEM, two independent biological in vivo experiments ( n = 6 mice per each group). *, p < 0.01; ns, not significant; two–tailed t -test.

    Techniques Used: Over Expression, In Vivo, Cell Counting, Staining, Microscopy, Two Tailed Test, Migration, Scratch-induced Wound-closure Assay, Generated, Imaging

    du145 htb 81 cells  (ATCC)


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    ATCC du145 htb 81 cells
    Du145 Htb 81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human pca cell lines du145 htb 81  (ATCC)


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    ATCC human pca cell lines du145 htb 81
    Human Pca Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC du145 htb 81 cells
    Du145 Htb 81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    du145 htb 81 cell lines  (ATCC)
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    ATCC du145 htb 81 cell lines
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    prostate cancer pca cell lines du145 atcc htb 81  (ATCC)
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    ATCC prostate cancer pca cell lines du145 atcc htb 81
    Advanced <t>prostate</t> <t>cancer</t> <t>cells</t> secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and <t>DU145</t> supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Prostate Cancer Pca Cell Lines Du145 Atcc Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell lines du145 htb 81  (ATCC)
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    ATCC cell lines du145 htb 81
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human pca cell lines du145 htb 81  (ATCC)
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    ATCC human pca cell lines du145 htb 81
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Human Pca Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques: Concentration Assay, Cell Culture

    The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques: Expressing, Cell Culture, Flow Cytometry

    Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques: De-Phosphorylation Assay, Cell Culture, Expressing, Western Blot, In-Cell ELISA, Activity Assay, Concentration Assay

    Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques:

    DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.

    Journal: PLoS ONE

    Article Title: Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

    doi: 10.1371/journal.pone.0064426

    Figure Lengend Snippet: DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.

    Article Snippet: The cell lines DU145 (HTB-81™) and PC3 (CRL-1435™) were purchased from ATCC®.

    Techniques: Staining, Incubation, Flow Cytometry