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Thermo Fisher dtt data
Dtt Data, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 83 stars, based on 1 article reviews
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dtt data - by Bioz Stars, 2020-03
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Isolation:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: Microarray study Amygdalae were isolated from wild-type (n=15) and neuropsin−/− (n=15) mice using a dissecting microscope in ice-cold ACSF (in mM: glucose (25), NaCl (115), NaH2 PO4 ·H2 O (1.2), KCl (3.3), CaCl2 , (2), MgSO4 , (1), NaHCO3 , (25.5), pH 7.4 and stored at −20°C in RNAlater solution (Qiagen). .. DTT data were transferred by Transfer Tool software (Affymetrix).

Microscopy:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: Microarray study Amygdalae were isolated from wild-type (n=15) and neuropsin−/− (n=15) mice using a dissecting microscope in ice-cold ACSF (in mM: glucose (25), NaCl (115), NaH2 PO4 ·H2 O (1.2), KCl (3.3), CaCl2 , (2), MgSO4 , (1), NaHCO3 , (25.5), pH 7.4 and stored at −20°C in RNAlater solution (Qiagen). .. DTT data were transferred by Transfer Tool software (Affymetrix).

Mouse Assay:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: Microarray study Amygdalae were isolated from wild-type (n=15) and neuropsin−/− (n=15) mice using a dissecting microscope in ice-cold ACSF (in mM: glucose (25), NaCl (115), NaH2 PO4 ·H2 O (1.2), KCl (3.3), CaCl2 , (2), MgSO4 , (1), NaHCO3 , (25.5), pH 7.4 and stored at −20°C in RNAlater solution (Qiagen). .. DTT data were transferred by Transfer Tool software (Affymetrix).

Electrophoresis:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen), the ribosomal fraction of RNA reduced with RiboMinus Kit (Invitrogen) and the RNA integrity verified by electrophoresis using Agilent Bioanalyser 2100 (Agilent Technologies). .. DTT data were transferred by Transfer Tool software (Affymetrix).

Microarray:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: Paragraph title: Microarray study ... DTT data were transferred by Transfer Tool software (Affymetrix).

Expressing:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: DTT data were transferred by Transfer Tool software (Affymetrix). .. Chip quality and raw microarray data pre-processing were performed according to the Affymetrix guidelines using Expression Console software (Affymetrix).

Chromatin Immunoprecipitation:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: DTT data were transferred by Transfer Tool software (Affymetrix). .. Chip quality and raw microarray data pre-processing were performed according to the Affymetrix guidelines using Expression Console software (Affymetrix).

Derivative Assay:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: DTT data were transferred by Transfer Tool software (Affymetrix). .. The obtained results were taken as the measure of mRNA abundance derived from the level of gene expression.

Software:

Article Title: Neuropsin cleaves EphB2 in the amygdala to control anxiety
Article Snippet: .. DTT data were transferred by Transfer Tool software (Affymetrix). .. Chip quality and raw microarray data pre-processing were performed according to the Affymetrix guidelines using Expression Console software (Affymetrix).

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    Thermo Fisher m dithiothreitol
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    M Dithiothreitol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Thermo Fisher
    Average 95 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-03
    95/100 stars
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    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) dithiothreitol. Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Redox Regulation of Methionine Aminopeptidase 2 Activity *

    doi: 10.1074/jbc.M114.554253

    Figure Lengend Snippet: Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) dithiothreitol. Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.

    Article Snippet: Just before use, the active site disulfide bond of the oxidoreductase was reduced with 50 m m dithiothreitol and 50 m m tris-(2-carboxyethyl)phosphine (TCEP) for 1 h at 25 °C, and the reducing agents were removed by two passes through Zeba spin desalting columns equilibrated with phosphate-buffered saline (Thermo Scientific).

    Techniques: Labeling, SDS Page