Structured Review

TaKaRa dsred express gene
Mixed infections . Ratio of wt L. monocytogenes strains and its inlA' mutant tagged with <t>YFP</t> (yellow), <t>DsRed</t> (red) or CFP (blue) prior to invasion (A) and after recovery from Caco-2 cells (B).
Dsred Express Gene, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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85/100 stars

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1) Product Images from "Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes"

Article Title: Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes

Journal: BMC Microbiology

doi: 10.1186/1471-2180-6-86

Mixed infections . Ratio of wt L. monocytogenes strains and its inlA' mutant tagged with YFP (yellow), DsRed (red) or CFP (blue) prior to invasion (A) and after recovery from Caco-2 cells (B).
Figure Legend Snippet: Mixed infections . Ratio of wt L. monocytogenes strains and its inlA' mutant tagged with YFP (yellow), DsRed (red) or CFP (blue) prior to invasion (A) and after recovery from Caco-2 cells (B).

Techniques Used: Mutagenesis

Related Articles

Amplification:

Article Title: Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes
Article Snippet: .. To construct a multicolour tagging system, the cfp ++ gene of pUC-cfp++ , the yfp ++ gene of pUC-yfp++ , the HcRed gene of pHcRed1 and the DsRed-Express gene of pDsRed-Express (Clontech Laboratories Inc., USA), were initially PCR amplified using either of the primersets Gfp_for/Gfp_rev, HcRed_for/HcRed_rev, or RedEx_for/DsRedEx_rev (Table ). .. The four resulting PCR products encoding cfp ++ , yfp ++ , HcRed and DsRed-Express respectively, were restricted with BamHI and PstI and subsequently ligated to the 7 Kb BamHI-PstI fragment of pNF8, to give pJEBAN2, pJEBAN3, pJEBAN4 and pJEBAN6.

Construct:

Article Title: Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes
Article Snippet: .. To construct a multicolour tagging system, the cfp ++ gene of pUC-cfp++ , the yfp ++ gene of pUC-yfp++ , the HcRed gene of pHcRed1 and the DsRed-Express gene of pDsRed-Express (Clontech Laboratories Inc., USA), were initially PCR amplified using either of the primersets Gfp_for/Gfp_rev, HcRed_for/HcRed_rev, or RedEx_for/DsRedEx_rev (Table ). .. The four resulting PCR products encoding cfp ++ , yfp ++ , HcRed and DsRed-Express respectively, were restricted with BamHI and PstI and subsequently ligated to the 7 Kb BamHI-PstI fragment of pNF8, to give pJEBAN2, pJEBAN3, pJEBAN4 and pJEBAN6.

Article Title: Viral complementation allows HIV-1 replication without integration
Article Snippet: .. DsRed-Express (DsRedX)-containing viruses were constructed in an identical manner using the DsRed-Express gene from Clontech. .. Replication-competent NLENG1-IRES (containing GFP), NLENY1-IRES (containing YFP), NLENC1-IRES (containing CFP) and NLRX-IRES (containing DsRedX)) and replication-defective viruses containing two stop codons in the envelope reading frame (NLENG1-ES-IRES, NLENY1-ES-IRES, NLENC1-ES-IRES and NLRX-ES-IRES) were constructed for each reporter virus [ ].

Polymerase Chain Reaction:

Article Title: Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes
Article Snippet: .. To construct a multicolour tagging system, the cfp ++ gene of pUC-cfp++ , the yfp ++ gene of pUC-yfp++ , the HcRed gene of pHcRed1 and the DsRed-Express gene of pDsRed-Express (Clontech Laboratories Inc., USA), were initially PCR amplified using either of the primersets Gfp_for/Gfp_rev, HcRed_for/HcRed_rev, or RedEx_for/DsRedEx_rev (Table ). .. The four resulting PCR products encoding cfp ++ , yfp ++ , HcRed and DsRed-Express respectively, were restricted with BamHI and PstI and subsequently ligated to the 7 Kb BamHI-PstI fragment of pNF8, to give pJEBAN2, pJEBAN3, pJEBAN4 and pJEBAN6.

Article Title: Long Range Regulation of Human FXN Gene Expression
Article Snippet: .. PCR primers EXT-BAC265-DSRED-F and EXT-DSRED-R-ADDITION were used to amplify a 1.5 kb PCR product containing the DsRed-Express gene and CMV promoter (pCMVIE) from the pDsRed-Express-N1 plasmid (Clontech, Palo Alto, CA). .. The 21 nucleotides (nt) at the 3′ end of EXT-BAC265-DSRED-F anneal to a unique sequence on the pDsRed-Express-N1 plasmid and the remaining 21 nt correspond to a sequence upstream of the chloramphenicol-resistance gene on the RP11-265B8::Ex5aEK BAC backbone.

Article Title: Protein targeting into secondary plastids of chlorarachniophytes
Article Snippet: .. A chimera fragment of Tgacp fused with the DsRed-Express gene (Clontech) was generated using the splicing by overlapped extension by PCR ( ); it was then inserted into the pSAG1/1 CAT, replacing CAT gene. .. The resulting fragment, which contained the SAG1 promoter, Tgacp fused with DsRed , and the SAG1 terminator, was ligated into the SacII site of pTub5CATSag1 vector.

Generated:

Article Title: Protein targeting into secondary plastids of chlorarachniophytes
Article Snippet: .. A chimera fragment of Tgacp fused with the DsRed-Express gene (Clontech) was generated using the splicing by overlapped extension by PCR ( ); it was then inserted into the pSAG1/1 CAT, replacing CAT gene. .. The resulting fragment, which contained the SAG1 promoter, Tgacp fused with DsRed , and the SAG1 terminator, was ligated into the SacII site of pTub5CATSag1 vector.

Modification:

Article Title: Transcriptional Switch of the dia1 and impA Promoter during the Growth/Differentiation Transition
Article Snippet: .. The DsRed Express gene (Clontech) was modified to carry a His6 tag (NH-Red) at the N terminus and ligated at the dia1 end of the intergenic region. .. Digestion of this construct with SspI left the fragment from bp 440 to 654 fragment ligated to NH-Red.

Chloramphenicol Acetyltransferase Assay:

Article Title: Protein targeting into secondary plastids of chlorarachniophytes
Article Snippet: .. A chimera fragment of Tgacp fused with the DsRed-Express gene (Clontech) was generated using the splicing by overlapped extension by PCR ( ); it was then inserted into the pSAG1/1 CAT, replacing CAT gene. .. The resulting fragment, which contained the SAG1 promoter, Tgacp fused with DsRed , and the SAG1 terminator, was ligated into the SacII site of pTub5CATSag1 vector.

Plasmid Preparation:

Article Title: Long Range Regulation of Human FXN Gene Expression
Article Snippet: .. PCR primers EXT-BAC265-DSRED-F and EXT-DSRED-R-ADDITION were used to amplify a 1.5 kb PCR product containing the DsRed-Express gene and CMV promoter (pCMVIE) from the pDsRed-Express-N1 plasmid (Clontech, Palo Alto, CA). .. The 21 nucleotides (nt) at the 3′ end of EXT-BAC265-DSRED-F anneal to a unique sequence on the pDsRed-Express-N1 plasmid and the remaining 21 nt correspond to a sequence upstream of the chloramphenicol-resistance gene on the RP11-265B8::Ex5aEK BAC backbone.

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  • 95
    TaKaRa pdsred2 mito
    Apposition of ER and mitochondria in M17 cells. A , Examples of colocalization of ER labeled with GFP-Sec61-β (green), and mitochondria labeled with <t>pDsRed2-mito</t> (red), in WT- and A30P-expressing M17 cells. B , Quantitation of colocalization (as in A ) by ImageJ analysis. Asterisk denotes significance versus EV.
    Pdsred2 Mito, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa plvx dsred monomer n1
    APN and EGFR synergistically activate PI3K/AKT and MEK/ERK1/2 signaling pathways. (A) IPEC-J2 cells were transfected with overexpression vector <t>pLVX-DsRed,</t> pLVX-APN-DsRed, pLVX-EGFR-DsRed, or pLVX-APN-DsRed + pLVX-EGFR-DsRed through lentiviral supernatant. Cells were infected with TGEV at an MOI of 2 and cultured for 30 min. Normal cells and TGEV infected-normal cells served as controls. The activation of downstream signaling pathways analyzed by Western-blot with anti-p-EGFR, anti-EGFR, anti-p-AKT, anti-AKT, anti-p-ERK1/2, anti-ERK1/2, and anti-GAPDH antibodies. (B) IPEC-J2 cells were transfected with interference vector pLVX-shRNA-APN, pLVX-shRNA-APNCtrl, pLVX-shRNA-EGFR, pLVX-shRNA-EGFRCtrl, or pLVX-shRNA-APN + pLVX-shRNA-EGFR through lentiviral supernatant. Cells were infected with TGEV at an MOI of 2, and cultured for 30 min Normal cells and TGEV infected-normal cells served as controls. The activation of downstream signaling pathways analyzed by Western-blot with anti-p-EGFR, anti-EGFR, anti-p-AKT, anti-AKT, anti-p-ERK1/2, anti-ERK1/2, and anti-GAPDH antibodies. (C–H) The ratio of p-EGFR, EGFR, p-AKT, AKT, p-ERK1/2 or ERK1/2 to GAPDH was normalized to control conditions. Data shown are the mean results ± SD from three independent experiments. (* 0.01
    Plvx Dsred Monomer N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa pdsred express c1
    AAV-mediated knockdown of MC4R expression in HEK-293 cells. (A) Schematic of the construct of the AAV vector. Top, AAV vector pTR-UF5 containing EGFP under the control of the CMV promoter and shRNA driven by the U6 promoter. Bottom, <t>pDsRed-Express-C1</t>
    Pdsred Express C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    TaKaRa plasmid pires2 dsred express2
    Decrease of lamotrigine-sensitive outward currents in TG neurons expressing MT TRESK subunits. A , Representative images of transfected TG neurons. TG neurons in the control group are transfected with the <t>pIRES2-DsRed-Express2</t> plasmid and express DsRed
    Plasmid Pires2 Dsred Express2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Apposition of ER and mitochondria in M17 cells. A , Examples of colocalization of ER labeled with GFP-Sec61-β (green), and mitochondria labeled with pDsRed2-mito (red), in WT- and A30P-expressing M17 cells. B , Quantitation of colocalization (as in A ) by ImageJ analysis. Asterisk denotes significance versus EV.

    Journal: The Journal of Neuroscience

    Article Title: ?-Synuclein Is Localized to Mitochondria-Associated ER Membranes

    doi: 10.1523/JNEUROSCI.2507-13.2014

    Figure Lengend Snippet: Apposition of ER and mitochondria in M17 cells. A , Examples of colocalization of ER labeled with GFP-Sec61-β (green), and mitochondria labeled with pDsRed2-mito (red), in WT- and A30P-expressing M17 cells. B , Quantitation of colocalization (as in A ) by ImageJ analysis. Asterisk denotes significance versus EV.

    Article Snippet: To investigate the effects of DRP1 on mitochondrial morphology, we cotransfected Drp1-WT and -KO MEFs with pDsRed2-Mito and α-syn-HA constructs.

    Techniques: Labeling, Expressing, Quantitation Assay

    Apposition of ER and mitochondria in HeLa cells. A , Examples of colocalization of ER labeled with GFP-Sec61-β (green), and mitochondria labeled with pDsRed2-mito (red), in HeLa cells transiently expressing WT- and A30P-α-syn. B , Quantitation of colocalization (as in A ) by ImageJ analysis, normalized to the baseline level of apposition value in EV cells (baseline = 100; average of 4 experiments ±SD). Asterisk denotes significance versus EV.

    Journal: The Journal of Neuroscience

    Article Title: ?-Synuclein Is Localized to Mitochondria-Associated ER Membranes

    doi: 10.1523/JNEUROSCI.2507-13.2014

    Figure Lengend Snippet: Apposition of ER and mitochondria in HeLa cells. A , Examples of colocalization of ER labeled with GFP-Sec61-β (green), and mitochondria labeled with pDsRed2-mito (red), in HeLa cells transiently expressing WT- and A30P-α-syn. B , Quantitation of colocalization (as in A ) by ImageJ analysis, normalized to the baseline level of apposition value in EV cells (baseline = 100; average of 4 experiments ±SD). Asterisk denotes significance versus EV.

    Article Snippet: To investigate the effects of DRP1 on mitochondrial morphology, we cotransfected Drp1-WT and -KO MEFs with pDsRed2-Mito and α-syn-HA constructs.

    Techniques: Labeling, Expressing, Quantitation Assay

    Mitochondrial fragmentation in M17 cells. A , Representative images of mitochondria in α-syn-expressing cells labeled with pDsRed2-Mito. Insets, Enlargements of the indicated regions (small boxes). B , Quantitation of fragmentation of the cells in A , measured as the percentage of all cells that contained predominantly fragmented (darker shading) versus tubular (lighter shading) mitochondria. Numbers within the boxes denote the average length (±SE) of the mitochondria in micrometers; n = number of mitochondria measured. *Significant difference versus EV ( p

    Journal: The Journal of Neuroscience

    Article Title: ?-Synuclein Is Localized to Mitochondria-Associated ER Membranes

    doi: 10.1523/JNEUROSCI.2507-13.2014

    Figure Lengend Snippet: Mitochondrial fragmentation in M17 cells. A , Representative images of mitochondria in α-syn-expressing cells labeled with pDsRed2-Mito. Insets, Enlargements of the indicated regions (small boxes). B , Quantitation of fragmentation of the cells in A , measured as the percentage of all cells that contained predominantly fragmented (darker shading) versus tubular (lighter shading) mitochondria. Numbers within the boxes denote the average length (±SE) of the mitochondria in micrometers; n = number of mitochondria measured. *Significant difference versus EV ( p

    Article Snippet: To investigate the effects of DRP1 on mitochondrial morphology, we cotransfected Drp1-WT and -KO MEFs with pDsRed2-Mito and α-syn-HA constructs.

    Techniques: Expressing, Labeling, Quantitation Assay

    Mitochondrial fragmentation in HeLa cells. A , Representative images of mitochondria in α-syn-expressing cells labeled with pDsRed2-Mito. Insets, Enlargements of the indicated regions (small boxes). B , Quantitation of fragmentation of the cells in A , measured as the percentage of all cells that contained predominantly fragmented tubular mitochondria. *Significant difference versus EV ( p

    Journal: The Journal of Neuroscience

    Article Title: ?-Synuclein Is Localized to Mitochondria-Associated ER Membranes

    doi: 10.1523/JNEUROSCI.2507-13.2014

    Figure Lengend Snippet: Mitochondrial fragmentation in HeLa cells. A , Representative images of mitochondria in α-syn-expressing cells labeled with pDsRed2-Mito. Insets, Enlargements of the indicated regions (small boxes). B , Quantitation of fragmentation of the cells in A , measured as the percentage of all cells that contained predominantly fragmented tubular mitochondria. *Significant difference versus EV ( p

    Article Snippet: To investigate the effects of DRP1 on mitochondrial morphology, we cotransfected Drp1-WT and -KO MEFs with pDsRed2-Mito and α-syn-HA constructs.

    Techniques: Expressing, Labeling, Quantitation Assay

    APN and EGFR synergistically activate PI3K/AKT and MEK/ERK1/2 signaling pathways. (A) IPEC-J2 cells were transfected with overexpression vector pLVX-DsRed, pLVX-APN-DsRed, pLVX-EGFR-DsRed, or pLVX-APN-DsRed + pLVX-EGFR-DsRed through lentiviral supernatant. Cells were infected with TGEV at an MOI of 2 and cultured for 30 min. Normal cells and TGEV infected-normal cells served as controls. The activation of downstream signaling pathways analyzed by Western-blot with anti-p-EGFR, anti-EGFR, anti-p-AKT, anti-AKT, anti-p-ERK1/2, anti-ERK1/2, and anti-GAPDH antibodies. (B) IPEC-J2 cells were transfected with interference vector pLVX-shRNA-APN, pLVX-shRNA-APNCtrl, pLVX-shRNA-EGFR, pLVX-shRNA-EGFRCtrl, or pLVX-shRNA-APN + pLVX-shRNA-EGFR through lentiviral supernatant. Cells were infected with TGEV at an MOI of 2, and cultured for 30 min Normal cells and TGEV infected-normal cells served as controls. The activation of downstream signaling pathways analyzed by Western-blot with anti-p-EGFR, anti-EGFR, anti-p-AKT, anti-AKT, anti-p-ERK1/2, anti-ERK1/2, and anti-GAPDH antibodies. (C–H) The ratio of p-EGFR, EGFR, p-AKT, AKT, p-ERK1/2 or ERK1/2 to GAPDH was normalized to control conditions. Data shown are the mean results ± SD from three independent experiments. (* 0.01

    Journal: Virology

    Article Title: Epidermal growth factor receptor is a co-factor for transmissible gastroenteritis virus entry

    doi: 10.1016/j.virol.2018.05.009

    Figure Lengend Snippet: APN and EGFR synergistically activate PI3K/AKT and MEK/ERK1/2 signaling pathways. (A) IPEC-J2 cells were transfected with overexpression vector pLVX-DsRed, pLVX-APN-DsRed, pLVX-EGFR-DsRed, or pLVX-APN-DsRed + pLVX-EGFR-DsRed through lentiviral supernatant. Cells were infected with TGEV at an MOI of 2 and cultured for 30 min. Normal cells and TGEV infected-normal cells served as controls. The activation of downstream signaling pathways analyzed by Western-blot with anti-p-EGFR, anti-EGFR, anti-p-AKT, anti-AKT, anti-p-ERK1/2, anti-ERK1/2, and anti-GAPDH antibodies. (B) IPEC-J2 cells were transfected with interference vector pLVX-shRNA-APN, pLVX-shRNA-APNCtrl, pLVX-shRNA-EGFR, pLVX-shRNA-EGFRCtrl, or pLVX-shRNA-APN + pLVX-shRNA-EGFR through lentiviral supernatant. Cells were infected with TGEV at an MOI of 2, and cultured for 30 min Normal cells and TGEV infected-normal cells served as controls. The activation of downstream signaling pathways analyzed by Western-blot with anti-p-EGFR, anti-EGFR, anti-p-AKT, anti-AKT, anti-p-ERK1/2, anti-ERK1/2, and anti-GAPDH antibodies. (C–H) The ratio of p-EGFR, EGFR, p-AKT, AKT, p-ERK1/2 or ERK1/2 to GAPDH was normalized to control conditions. Data shown are the mean results ± SD from three independent experiments. (* 0.01

    Article Snippet: 2.4 Plasmid construction and DNA transfection The pLVX-DsRed-Monomer-N1 is an HIV-1-based, lentiviral expression vector that expresses the gene of interest fused to the DsRed-Monomer (Clontech, Palo Aito, CA).

    Techniques: Transfection, Over Expression, Plasmid Preparation, Infection, Cell Culture, Activation Assay, Western Blot, shRNA

    AAV-mediated knockdown of MC4R expression in HEK-293 cells. (A) Schematic of the construct of the AAV vector. Top, AAV vector pTR-UF5 containing EGFP under the control of the CMV promoter and shRNA driven by the U6 promoter. Bottom, pDsRed-Express-C1

    Journal:

    Article Title: Adeno-associated virus-mediated knockdown of melanocortin-4 receptor in the paraventricular nucleus of the hypothalamus promotes high-fat diet-induced hyperphagia and obesity

    doi: 10.1677/JOE-08-0009

    Figure Lengend Snippet: AAV-mediated knockdown of MC4R expression in HEK-293 cells. (A) Schematic of the construct of the AAV vector. Top, AAV vector pTR-UF5 containing EGFP under the control of the CMV promoter and shRNA driven by the U6 promoter. Bottom, pDsRed-Express-C1

    Article Snippet: MC4R was cloned into the pDsRed-Express-C1, a mammalian expression vector that encodes DsRed-Express, a variant of red fluorescent protein (Clontech).

    Techniques: Expressing, Construct, Plasmid Preparation, shRNA

    Decrease of lamotrigine-sensitive outward currents in TG neurons expressing MT TRESK subunits. A , Representative images of transfected TG neurons. TG neurons in the control group are transfected with the pIRES2-DsRed-Express2 plasmid and express DsRed

    Journal: The Journal of Neuroscience

    Article Title: Functional Analysis of a Migraine-Associated TRESK K+ Channel Mutation

    doi: 10.1523/JNEUROSCI.1237-13.2013

    Figure Lengend Snippet: Decrease of lamotrigine-sensitive outward currents in TG neurons expressing MT TRESK subunits. A , Representative images of transfected TG neurons. TG neurons in the control group are transfected with the pIRES2-DsRed-Express2 plasmid and express DsRed

    Article Snippet: The coding region of the mouse TRESK K+ channel (encoded by the kcnk18 gene) was PCR amplified from a plasmid purchased from Open Biosystems and cloned into the plasmid pIRES2-DsRed-Express2 (Clontech) to generate the construct wtTRESK-IRES-DsRed.

    Techniques: Expressing, Transfection, Plasmid Preparation