Structured Review

Millipore drugs mk 801
Effects of antipsychotics on hyperlocomotion induced by <t>MK-801.</t> Each symbol represents mean ± SEM distance traveled ( n = 7–8) in the 16–45-min period after MK-801 administration. Test compound or vehicle was administered 45 min before MK-801 (0.3 mg/kg). * p
Drugs Mk 801, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/drugs mk 801/product/Millipore
Average 97 stars, based on 7 article reviews
Price from $9.99 to $1999.99
drugs mk 801 - by Bioz Stars, 2022-09
97/100 stars

Images

1) Product Images from "Antipsychotic, antidepressant, and cognitive-impairment properties of antipsychotics: rat profile and implications for behavioral and psychological symptoms of dementia"

Article Title: Antipsychotic, antidepressant, and cognitive-impairment properties of antipsychotics: rat profile and implications for behavioral and psychological symptoms of dementia

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

doi: 10.1007/s00210-014-0966-4

Effects of antipsychotics on hyperlocomotion induced by MK-801. Each symbol represents mean ± SEM distance traveled ( n = 7–8) in the 16–45-min period after MK-801 administration. Test compound or vehicle was administered 45 min before MK-801 (0.3 mg/kg). * p
Figure Legend Snippet: Effects of antipsychotics on hyperlocomotion induced by MK-801. Each symbol represents mean ± SEM distance traveled ( n = 7–8) in the 16–45-min period after MK-801 administration. Test compound or vehicle was administered 45 min before MK-801 (0.3 mg/kg). * p

Techniques Used:

2) Product Images from "Chronic MK-801 Application in Adolescence and Early Adulthood: A Spatial Working Memory Deficit in Adult Long-Evans Rats But No Changes in the Hippocampal NMDA Receptor Subunits"

Article Title: Chronic MK-801 Application in Adolescence and Early Adulthood: A Spatial Working Memory Deficit in Adult Long-Evans Rats But No Changes in the Hippocampal NMDA Receptor Subunits

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2018.00042

Latency and path to the platform (distance) in the water maze summed for swim 2, 3, and 4, and averaged across the 10 days of training. In the Long-Evans PD 60 cohort, MK-801 treated rats did not search for the platform on repeated swims as efficiently as saline treated rats ( ∗ P
Figure Legend Snippet: Latency and path to the platform (distance) in the water maze summed for swim 2, 3, and 4, and averaged across the 10 days of training. In the Long-Evans PD 60 cohort, MK-801 treated rats did not search for the platform on repeated swims as efficiently as saline treated rats ( ∗ P

Techniques Used:

Performance in the elevated plus maze evaluated by total time spent in the central part (A) , open arms (B) , and closed arms (C) . Comparing MK-801 and saline-treated rats in each cohort yielded significant differences only for PD 30 Long-Evans rats in entering the center, and for PD 30 Long-Evans rats in entering the closed arms. Group means ± SEM ( ∗ P
Figure Legend Snippet: Performance in the elevated plus maze evaluated by total time spent in the central part (A) , open arms (B) , and closed arms (C) . Comparing MK-801 and saline-treated rats in each cohort yielded significant differences only for PD 30 Long-Evans rats in entering the center, and for PD 30 Long-Evans rats in entering the closed arms. Group means ± SEM ( ∗ P

Techniques Used:

Performance in active place avoidance evaluated as the summed number of entrances into the to-be-avoided sector during 4 days of acquisition (left graphs) and 1st day of reversal (right graphs). Differences between MK-801 and saline treated rats were statistically significant only during reversal in the Long-Evans PD 60 cohort, with MK-801 administered rats to be superior to saline controls ( ∗ P
Figure Legend Snippet: Performance in active place avoidance evaluated as the summed number of entrances into the to-be-avoided sector during 4 days of acquisition (left graphs) and 1st day of reversal (right graphs). Differences between MK-801 and saline treated rats were statistically significant only during reversal in the Long-Evans PD 60 cohort, with MK-801 administered rats to be superior to saline controls ( ∗ P

Techniques Used:

Examples of Western blot of the hippocampal supernatant showing expression of the NMDA subunits (GluN1 ∼100 kDa, GluN2A∼170 kDa, GluN2B ∼180 kDa) at two age intervals (PD 30, PD 60) in Long-Evans and Wistar rats. Control group samples are in columns 1–4 and MK-801 samples are in the columns 5–8.
Figure Legend Snippet: Examples of Western blot of the hippocampal supernatant showing expression of the NMDA subunits (GluN1 ∼100 kDa, GluN2A∼170 kDa, GluN2B ∼180 kDa) at two age intervals (PD 30, PD 60) in Long-Evans and Wistar rats. Control group samples are in columns 1–4 and MK-801 samples are in the columns 5–8.

Techniques Used: Western Blot, Expressing

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Millipore mice mk 801
    Effects of haloperidol, olanzapine, ziprasidone, and  PHA ‐543613 on spatial learning and memory in  MK ‐801‐treated mice in the Morris water maze test. (a) Mean escape latency of reaching the submerged platform in the training period. (b) Crossing platform area times in the probe test. Data were expressed as mean ±  SEM  ( n  = 8/group). * p
    Mice Mk 801, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mice mk 801/product/Millipore
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mice mk 801 - by Bioz Stars, 2022-09
    97/100 stars
      Buy from Supplier

    96
    Millipore memantine
    PCP and MK801—but not <t>memantine,</t> saline or LSD—induce RSP-localized rhythms. a , Quantification of oscillation frequency content before and after PCP injection, n = 5 mice. b , For PCP, example temporal video sequence of 410 nm-corrected fluorescence over 1 s in a Thy1-GCaMP6s mouse showing RSP rhythm. c , Quantification of oscillation frequency content before and after MK801 injection, n = 5 mice. d , For MK801, example temporal video sequence of 410 nm-corrected fluorescence over 1 s in a Thy1-GCaMP6s mouse, showing RSP rhythm. e , Quantification of oscillation frequency content before and after memantine injection, n = 5 mice. f , For memantine, example temporal video sequence of 410 nm-corrected fluorescence across 1 s in a Thy1-GCaMP6s mouse. Here, neither rhythms nor travelling waves are seen. g , Quantification of oscillation frequency content before and after saline injection, n = 5 mice. h , Quantification of mean power in 1–3 Hz band for saline ( n = 5 mice), corrected two-sided paired t -tests (* P
    Memantine, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/memantine/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    memantine - by Bioz Stars, 2022-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Effects of haloperidol, olanzapine, ziprasidone, and  PHA ‐543613 on spatial learning and memory in  MK ‐801‐treated mice in the Morris water maze test. (a) Mean escape latency of reaching the submerged platform in the training period. (b) Crossing platform area times in the probe test. Data were expressed as mean ±  SEM  ( n  = 8/group). * p

    Journal: Brain and Behavior

    Article Title: Effects of haloperidol, olanzapine, ziprasidone, and PHA‐543613 on spatial learning and memory in the Morris water maze test in naïve and MK‐801‐treated mice. Effects of haloperidol, olanzapine, ziprasidone, and PHA‐543613 on spatial learning and memory in the Morris water maze test in naïve and MK‐801‐treated mice

    doi: 10.1002/brb3.764

    Figure Lengend Snippet: Effects of haloperidol, olanzapine, ziprasidone, and PHA ‐543613 on spatial learning and memory in MK ‐801‐treated mice in the Morris water maze test. (a) Mean escape latency of reaching the submerged platform in the training period. (b) Crossing platform area times in the probe test. Data were expressed as mean ±  SEM ( n  = 8/group). * p

    Article Snippet: 2.3 Drugs and treatment of mice MK‐801 and PHA‐543613 were purchased from Sigma‐Aldrich (St. Louis, MO, USA).

    Techniques: Mouse Assay

    Effects of haloperidol, olanzapine, ziprasidone, and  PHA ‐543613 on swimming speed in naïve and  MK ‐801‐treated mice. (a) Swimming speed of naïve mice in training period. (b) Swimming speed of  MK ‐801‐treated mice in training period. Data were expressed as mean ±  SEM  ( n  = 8/group)

    Journal: Brain and Behavior

    Article Title: Effects of haloperidol, olanzapine, ziprasidone, and PHA‐543613 on spatial learning and memory in the Morris water maze test in naïve and MK‐801‐treated mice. Effects of haloperidol, olanzapine, ziprasidone, and PHA‐543613 on spatial learning and memory in the Morris water maze test in naïve and MK‐801‐treated mice

    doi: 10.1002/brb3.764

    Figure Lengend Snippet: Effects of haloperidol, olanzapine, ziprasidone, and PHA ‐543613 on swimming speed in naïve and MK ‐801‐treated mice. (a) Swimming speed of naïve mice in training period. (b) Swimming speed of MK ‐801‐treated mice in training period. Data were expressed as mean ±  SEM ( n  = 8/group)

    Article Snippet: 2.3 Drugs and treatment of mice MK‐801 and PHA‐543613 were purchased from Sigma‐Aldrich (St. Louis, MO, USA).

    Techniques: Mouse Assay

    Plasma corticosterone at expression. Plasma corticosterone levels at the expression phase. All groups (n = 12) were habituated to the chamber for 30 min prior to receiving a challenge injection of MK-801 at T0. Corticosterone levels were unaltered by any prior treatment either at baseline (T-30) or 60min after MK-801 challenge (T60). Corticosterone levels did not significantly increase from baseline and were not affected by prior induction treatment of RU486 or MK-801. Data are presented as the mean ± SEM.

    Journal: PLoS ONE

    Article Title: Effect of the glucocorticoid receptor antagonist RU486 on MK-801 induced behavioural sensitisation

    doi: 10.1371/journal.pone.0176156

    Figure Lengend Snippet: Plasma corticosterone at expression. Plasma corticosterone levels at the expression phase. All groups (n = 12) were habituated to the chamber for 30 min prior to receiving a challenge injection of MK-801 at T0. Corticosterone levels were unaltered by any prior treatment either at baseline (T-30) or 60min after MK-801 challenge (T60). Corticosterone levels did not significantly increase from baseline and were not affected by prior induction treatment of RU486 or MK-801. Data are presented as the mean ± SEM.

    Article Snippet: Preparation of drugs and administration procedure MK-801 (Sigma) was dissolved in 0.9% NaCl to a concentration of 0.25mg/ml.

    Techniques: Expressing, Injection

    Experimental timeline. Timeline of the study. Behavioural testing was completed on the first cohort of animals (a) and corticosterone analysis on the second cohort (b). During the induction phase (Days 1–7) rats were sensitised with injections of MK-801 (0.25mg/kg) or Saline. Thirty min prior to these injections either RU486 (20mg/kg) or Vehicle (DMSO) was administered. In the second cohort blood samples were collected from the saphenous vein on Days 1, 4 and 7. The time points are relative to the MK-801 or saline injection at T0. The first sample was collected at T-60 min prior to habituation to the test chamber. After 30 min habituation (T-30) rats were injected with RU486 or Vehicle and the next sample was taken after 30min at T0. Rats were then injected with MK-801 or Saline and the third blood sample was collected 60min later at T60. At day 13, all rats were administered MK-801 to test for the expression of sensitisation. The first blood sample was taken at T-30, prior to habitation to the test chamber. At T0 rats were administered with MK-801 and the second blood sample was collected 60min later at T60.

    Journal: PLoS ONE

    Article Title: Effect of the glucocorticoid receptor antagonist RU486 on MK-801 induced behavioural sensitisation

    doi: 10.1371/journal.pone.0176156

    Figure Lengend Snippet: Experimental timeline. Timeline of the study. Behavioural testing was completed on the first cohort of animals (a) and corticosterone analysis on the second cohort (b). During the induction phase (Days 1–7) rats were sensitised with injections of MK-801 (0.25mg/kg) or Saline. Thirty min prior to these injections either RU486 (20mg/kg) or Vehicle (DMSO) was administered. In the second cohort blood samples were collected from the saphenous vein on Days 1, 4 and 7. The time points are relative to the MK-801 or saline injection at T0. The first sample was collected at T-60 min prior to habituation to the test chamber. After 30 min habituation (T-30) rats were injected with RU486 or Vehicle and the next sample was taken after 30min at T0. Rats were then injected with MK-801 or Saline and the third blood sample was collected 60min later at T60. At day 13, all rats were administered MK-801 to test for the expression of sensitisation. The first blood sample was taken at T-30, prior to habitation to the test chamber. At T0 rats were administered with MK-801 and the second blood sample was collected 60min later at T60.

    Article Snippet: Preparation of drugs and administration procedure MK-801 (Sigma) was dissolved in 0.9% NaCl to a concentration of 0.25mg/ml.

    Techniques: Injection, Expressing

    Plasma corticosterone at induction. Plasma corticosterone levels throughout induction were measured every third day. (a) Rats (n = 12/group) were bled prior to treatment (T-60) for baseline measures. Corticosterone levels at baseline decreased over the 7-day induction period. (b) After 30min habituation to the chamber half the animals were injected with RU486 and half with vehicle. Animals were then bled 30min later (T0). At this time point RU486 was shown to increase plasma corticosterone levels compared to vehicle groups across all days. A main effect of Day was also observed at this time point. (c) Immediately after being bled at T0 rats were injected with MK-801 (or saline) and a final blood sample was collected 60min later (T60). At the T60 time point on Day 1 there was a main effect of both RU486 and MK-801. A post-hoc Dunnett’s test on the repeated measures ANOVA indicate that the RU486+MK-801 group had significantly elevated plasma corticosterone levels across all days. * p

    Journal: PLoS ONE

    Article Title: Effect of the glucocorticoid receptor antagonist RU486 on MK-801 induced behavioural sensitisation

    doi: 10.1371/journal.pone.0176156

    Figure Lengend Snippet: Plasma corticosterone at induction. Plasma corticosterone levels throughout induction were measured every third day. (a) Rats (n = 12/group) were bled prior to treatment (T-60) for baseline measures. Corticosterone levels at baseline decreased over the 7-day induction period. (b) After 30min habituation to the chamber half the animals were injected with RU486 and half with vehicle. Animals were then bled 30min later (T0). At this time point RU486 was shown to increase plasma corticosterone levels compared to vehicle groups across all days. A main effect of Day was also observed at this time point. (c) Immediately after being bled at T0 rats were injected with MK-801 (or saline) and a final blood sample was collected 60min later (T60). At the T60 time point on Day 1 there was a main effect of both RU486 and MK-801. A post-hoc Dunnett’s test on the repeated measures ANOVA indicate that the RU486+MK-801 group had significantly elevated plasma corticosterone levels across all days. * p

    Article Snippet: Preparation of drugs and administration procedure MK-801 (Sigma) was dissolved in 0.9% NaCl to a concentration of 0.25mg/ml.

    Techniques: Injection

    Effect of RU486 on MK-801 behavioural sensitisation. MK-801-induced locomotor sensitisation. (a) During the induction phase, rats received daily administration of RU486 (20mg/kg) (grey symbols) or DMSO vehicle (white symbols) . Thirty minutes later they were injected with MK-801 (triangles) (0.25mg/kg) or saline (circles) (n = 4-8/group). Locomotor activity is recorded as the distance travelled over 120min. Not surprisingly locomotion was increased by repeated MK-801 but not saline. RU486 had no effect on the saline exposed animals but produced an additive locomotor effect in the MK-801 treated animals that progressively increased across the induction phase (* p

    Journal: PLoS ONE

    Article Title: Effect of the glucocorticoid receptor antagonist RU486 on MK-801 induced behavioural sensitisation

    doi: 10.1371/journal.pone.0176156

    Figure Lengend Snippet: Effect of RU486 on MK-801 behavioural sensitisation. MK-801-induced locomotor sensitisation. (a) During the induction phase, rats received daily administration of RU486 (20mg/kg) (grey symbols) or DMSO vehicle (white symbols) . Thirty minutes later they were injected with MK-801 (triangles) (0.25mg/kg) or saline (circles) (n = 4-8/group). Locomotor activity is recorded as the distance travelled over 120min. Not surprisingly locomotion was increased by repeated MK-801 but not saline. RU486 had no effect on the saline exposed animals but produced an additive locomotor effect in the MK-801 treated animals that progressively increased across the induction phase (* p

    Article Snippet: Preparation of drugs and administration procedure MK-801 (Sigma) was dissolved in 0.9% NaCl to a concentration of 0.25mg/ml.

    Techniques: Injection, Activity Assay, Produced

    Dopamine and metabolite levels in the NAc. Nucleus accumbens tissue samples were collected one day after the final MK-801 challenge (n = 12/group). Dopamine (a) and its major metabolites DOPAC (b) and HVA (c) were significantly elevated in RU486 pre-treated rats (grey bars) . Since DOPAC and HVA were both increased there was no difference in the DOPAC/HVA ratio between groups (d). Data are presented as the mean ± SEM * p

    Journal: PLoS ONE

    Article Title: Effect of the glucocorticoid receptor antagonist RU486 on MK-801 induced behavioural sensitisation

    doi: 10.1371/journal.pone.0176156

    Figure Lengend Snippet: Dopamine and metabolite levels in the NAc. Nucleus accumbens tissue samples were collected one day after the final MK-801 challenge (n = 12/group). Dopamine (a) and its major metabolites DOPAC (b) and HVA (c) were significantly elevated in RU486 pre-treated rats (grey bars) . Since DOPAC and HVA were both increased there was no difference in the DOPAC/HVA ratio between groups (d). Data are presented as the mean ± SEM * p

    Article Snippet: Preparation of drugs and administration procedure MK-801 (Sigma) was dissolved in 0.9% NaCl to a concentration of 0.25mg/ml.

    Techniques:

    Mammillary body mediates MK-801 induced state-dependent fear. ( a ) Schema for chemogenetic inhibition of MB. ( b ) Representative image of virus expression in the MB. Red, hM4D-mCherry. Scale bar, 200 μm. ( c ) Representative images showed that CNO reduced c-Fos level in the MB expressing hM4D while not that injected with the control virus. Mice injected with either vehicle or CNO (2 mg/kg) in their homecages were perfused 2 hours later. Brains were processed for c-Fos analysis. Arrows label the c-Fos+ virus- neurons and arrow heads label the c-Fos+ virus+ neurons in the MB. Green, c-Fos. Red, hM4D/EGFP. Scale bar, 50 μm. ( d ) CNO reduced the percentage of c-Fos-positive neurons in the hM4D-expressing MB cells ( n = 4, 5, 4 mice, one-way ANOVA followed by Tukey’s test). ( e ) CNO reduced c-Fos level in MB expressing hM4D ( n = 4, 5, 4 mice, one-way ANOVA followed by Tukey’s test). ( f ) CNO administration either before training or before test had no effect on the fear memory in the control group ( n = 7, 9, 9 mice, one-way ANOVA followed by Tukey’s test). ( g ) Mice expressing hM4D in the MB injected CNO before training only froze when tested under CNO ( n = 9, 11 mice, two-way ANOVA followed by Sidak’s test). ( h ) Mice injected MK-801 before training showed robust freezing when tested either under MK-801 or in the MB-inhibition state but not in the vehicle-injection condition ( n = 8, 9, 7 mice, one-way ANOVA followed by Tukey’s test). ( i ) MK-801 injection before test significantly reduced the freezing level of mice trained in the normal condition. Whereas, MK-801 injection before test in mice both trained and tested under the MB inhibition condition had no effect on fear behavior ( n = 8, 9 mice, two-way ANOVA followed by Sidak’s test). ( j ) Chemogenetic inhibition of MB had no effect on the basal locomotion and foot-shock-induced hyperactivity in mice ( n = 17 mice per group, two-way ANOVA followed by Sidak’s test). ns: P > 0.05, * P

    Journal: Scientific Reports

    Article Title: Mammillary body regulates state-dependent fear by alternating cortical oscillations

    doi: 10.1038/s41598-018-31622-z

    Figure Lengend Snippet: Mammillary body mediates MK-801 induced state-dependent fear. ( a ) Schema for chemogenetic inhibition of MB. ( b ) Representative image of virus expression in the MB. Red, hM4D-mCherry. Scale bar, 200 μm. ( c ) Representative images showed that CNO reduced c-Fos level in the MB expressing hM4D while not that injected with the control virus. Mice injected with either vehicle or CNO (2 mg/kg) in their homecages were perfused 2 hours later. Brains were processed for c-Fos analysis. Arrows label the c-Fos+ virus- neurons and arrow heads label the c-Fos+ virus+ neurons in the MB. Green, c-Fos. Red, hM4D/EGFP. Scale bar, 50 μm. ( d ) CNO reduced the percentage of c-Fos-positive neurons in the hM4D-expressing MB cells ( n = 4, 5, 4 mice, one-way ANOVA followed by Tukey’s test). ( e ) CNO reduced c-Fos level in MB expressing hM4D ( n = 4, 5, 4 mice, one-way ANOVA followed by Tukey’s test). ( f ) CNO administration either before training or before test had no effect on the fear memory in the control group ( n = 7, 9, 9 mice, one-way ANOVA followed by Tukey’s test). ( g ) Mice expressing hM4D in the MB injected CNO before training only froze when tested under CNO ( n = 9, 11 mice, two-way ANOVA followed by Sidak’s test). ( h ) Mice injected MK-801 before training showed robust freezing when tested either under MK-801 or in the MB-inhibition state but not in the vehicle-injection condition ( n = 8, 9, 7 mice, one-way ANOVA followed by Tukey’s test). ( i ) MK-801 injection before test significantly reduced the freezing level of mice trained in the normal condition. Whereas, MK-801 injection before test in mice both trained and tested under the MB inhibition condition had no effect on fear behavior ( n = 8, 9 mice, two-way ANOVA followed by Sidak’s test). ( j ) Chemogenetic inhibition of MB had no effect on the basal locomotion and foot-shock-induced hyperactivity in mice ( n = 17 mice per group, two-way ANOVA followed by Sidak’s test). ns: P > 0.05, * P

    Article Snippet: Drug injections MK-801 (Sigma-Aldrich) was made in a stock solution of 5 mg/ml in ethanol and diluted in PBS to desired concentrations.

    Techniques: Inhibition, Expressing, Injection, Mouse Assay

    MK-801 induces state-dependent fear memory and enhances cortical delta oscillations. ( a ) Effect of different doses of MK-801 injected (i.p.) 30 min before fear conditioning on freezing ( n = 8, 8, 9 mice, one-way ANOVA followed by Tukey’s test). ( b ) Effect of different doses of MK-801 injected (i.p.) 30 min before test on freezing ( n = 7, 13, 9 mice, one-way ANOVA followed by Tukey’s test). ( c ) Effect of MK-801 injected (0.1 mg/kg) both before conditioning and before test on freezing ( n = 8, 8 mice, unpaired t test). ( d ) Mice injected with vehicle/MK-801 before training only froze when tested under the same vehicle/MK-801 condition ( n = 7, 9, 11 mice, two-way ANOVA followed by Sidak’s test). ( e ) Mice trained with MK-801 (0.1 mg/kg) showed robust freezing when tested with MK-801 ( n = 11 mice, one-way ANOVA followed by Tukey’s test). ( f ) MK-801 when injected at the dose of 0.2 mg/kg impaired fear memory in mice ( n = 7, 10, 13 mice, one-way ANOVA followed by Tukey’s test). ( g ) Mean speed measured without or with foot-shock after the injection of MK-801 (i.p.) at different doses ( n = 18, 14, 7 mice, two-way ANOVA followed by Tukey’s test). ( h ) Representative raw traces of cortical LFPs under the basal (black) and the MK-801 (red) conditions. ( i ) One example of the temporal/spectral structures of cortical LFPs under the influence of MK-801 (0.1 mg/kg, i.p.). Black/red box (5 min) labels the signal used as baseline/MK-801 onboard in the following statistical analysis. ( j ) Average power spectrum of the baseline and the MK-801 onboard periods ( n = 12 sites from 7 mice). Shadow areas indicate SEM. ( k ) Quantification of the average power of delta band (2–4 Hz) during the baseline and the MK-801 onboard periods ( n = 12 sites from 7 mice, paired t test). ( l ) Illustration of MK-801 induced brain state transitions. ns: P > 0.05, * P

    Journal: Scientific Reports

    Article Title: Mammillary body regulates state-dependent fear by alternating cortical oscillations

    doi: 10.1038/s41598-018-31622-z

    Figure Lengend Snippet: MK-801 induces state-dependent fear memory and enhances cortical delta oscillations. ( a ) Effect of different doses of MK-801 injected (i.p.) 30 min before fear conditioning on freezing ( n = 8, 8, 9 mice, one-way ANOVA followed by Tukey’s test). ( b ) Effect of different doses of MK-801 injected (i.p.) 30 min before test on freezing ( n = 7, 13, 9 mice, one-way ANOVA followed by Tukey’s test). ( c ) Effect of MK-801 injected (0.1 mg/kg) both before conditioning and before test on freezing ( n = 8, 8 mice, unpaired t test). ( d ) Mice injected with vehicle/MK-801 before training only froze when tested under the same vehicle/MK-801 condition ( n = 7, 9, 11 mice, two-way ANOVA followed by Sidak’s test). ( e ) Mice trained with MK-801 (0.1 mg/kg) showed robust freezing when tested with MK-801 ( n = 11 mice, one-way ANOVA followed by Tukey’s test). ( f ) MK-801 when injected at the dose of 0.2 mg/kg impaired fear memory in mice ( n = 7, 10, 13 mice, one-way ANOVA followed by Tukey’s test). ( g ) Mean speed measured without or with foot-shock after the injection of MK-801 (i.p.) at different doses ( n = 18, 14, 7 mice, two-way ANOVA followed by Tukey’s test). ( h ) Representative raw traces of cortical LFPs under the basal (black) and the MK-801 (red) conditions. ( i ) One example of the temporal/spectral structures of cortical LFPs under the influence of MK-801 (0.1 mg/kg, i.p.). Black/red box (5 min) labels the signal used as baseline/MK-801 onboard in the following statistical analysis. ( j ) Average power spectrum of the baseline and the MK-801 onboard periods ( n = 12 sites from 7 mice). Shadow areas indicate SEM. ( k ) Quantification of the average power of delta band (2–4 Hz) during the baseline and the MK-801 onboard periods ( n = 12 sites from 7 mice, paired t test). ( l ) Illustration of MK-801 induced brain state transitions. ns: P > 0.05, * P

    Article Snippet: Drug injections MK-801 (Sigma-Aldrich) was made in a stock solution of 5 mg/ml in ethanol and diluted in PBS to desired concentrations.

    Techniques: Injection, Mouse Assay

    Memory formed under MK-801 is able to be retrieved by inducing delta oscillations in neocortex. ( a ) Experimental design for testing memory transfer between the MK-801-induced state and state induced by 3 Hz stimulation. Thy1-ChR2 mice were trained (2 s, 0.6 mA, 3 foot shocks) under MK-801 and memory test was performed under different conditions. ( b ) Mice showed increased freezing during the 3-min-light-on epochs when tested in the conditioning context ( n = 8 mice, one-way ANOVA followed by Tukey’s test). ( c ) Freezing behavior during the light-on epochs was as robust as that tested under MK-801 ( n = 8 mice, paired t test). ( d ) Mice did not show light-associated increase of freezing behavior when tested in a novel context ( n 8 mice, paired t test). ns: P > 0.05, * P

    Journal: Scientific Reports

    Article Title: Mammillary body regulates state-dependent fear by alternating cortical oscillations

    doi: 10.1038/s41598-018-31622-z

    Figure Lengend Snippet: Memory formed under MK-801 is able to be retrieved by inducing delta oscillations in neocortex. ( a ) Experimental design for testing memory transfer between the MK-801-induced state and state induced by 3 Hz stimulation. Thy1-ChR2 mice were trained (2 s, 0.6 mA, 3 foot shocks) under MK-801 and memory test was performed under different conditions. ( b ) Mice showed increased freezing during the 3-min-light-on epochs when tested in the conditioning context ( n = 8 mice, one-way ANOVA followed by Tukey’s test). ( c ) Freezing behavior during the light-on epochs was as robust as that tested under MK-801 ( n = 8 mice, paired t test). ( d ) Mice did not show light-associated increase of freezing behavior when tested in a novel context ( n 8 mice, paired t test). ns: P > 0.05, * P

    Article Snippet: Drug injections MK-801 (Sigma-Aldrich) was made in a stock solution of 5 mg/ml in ethanol and diluted in PBS to desired concentrations.

    Techniques: Mouse Assay

    Activity of the mammillary body is repressed upon MK-801 administration. ( a ) Representative images of c-Fos immunostaining in the MB, CA1, DG and septum under the vehicle and the MK-801 (i.p., 0.1 mg/kg) conditions. Green, c-Fos. Blue, DAPI. Scale bar, 200 μm. ( b ) MK-801 reduced the expression of c-Fos in the MB, CA1 and DG, but not septum ( n = 5, 4 mice, unpaired t test). ( c ) Left, schema showing injection of AAV8: hSyn-GCaMP6f and subsequent fiber implantation into the MB. Right bottom, one example image of virus expression in the MB. Green, GCaMP6f. Scale bar, 200 μm. ( d ) Top, representative traces of calcium transients in the MB before and after (30 min later) vehicle (left) or MK-801(right) injection. Bottom left: injection of vehicle had no effect on the basal activity in the MB. Bottom right: MK-801 reduced both the amplitude and the frequency of the calcium transients in the MB ( n = 7 from 5 mice each, paired t test) (Each point represents a normalized average signal of 5 min. Time of injection: 0 min; baseline: - 5 to 0 min; vehicle/MK-801: 30 to 35 min). ns: P > 0.05, ** P

    Journal: Scientific Reports

    Article Title: Mammillary body regulates state-dependent fear by alternating cortical oscillations

    doi: 10.1038/s41598-018-31622-z

    Figure Lengend Snippet: Activity of the mammillary body is repressed upon MK-801 administration. ( a ) Representative images of c-Fos immunostaining in the MB, CA1, DG and septum under the vehicle and the MK-801 (i.p., 0.1 mg/kg) conditions. Green, c-Fos. Blue, DAPI. Scale bar, 200 μm. ( b ) MK-801 reduced the expression of c-Fos in the MB, CA1 and DG, but not septum ( n = 5, 4 mice, unpaired t test). ( c ) Left, schema showing injection of AAV8: hSyn-GCaMP6f and subsequent fiber implantation into the MB. Right bottom, one example image of virus expression in the MB. Green, GCaMP6f. Scale bar, 200 μm. ( d ) Top, representative traces of calcium transients in the MB before and after (30 min later) vehicle (left) or MK-801(right) injection. Bottom left: injection of vehicle had no effect on the basal activity in the MB. Bottom right: MK-801 reduced both the amplitude and the frequency of the calcium transients in the MB ( n = 7 from 5 mice each, paired t test) (Each point represents a normalized average signal of 5 min. Time of injection: 0 min; baseline: - 5 to 0 min; vehicle/MK-801: 30 to 35 min). ns: P > 0.05, ** P

    Article Snippet: Drug injections MK-801 (Sigma-Aldrich) was made in a stock solution of 5 mg/ml in ethanol and diluted in PBS to desired concentrations.

    Techniques: Activity Assay, Immunostaining, Expressing, Mouse Assay, Injection

    PCP and MK801—but not memantine, saline or LSD—induce RSP-localized rhythms. a , Quantification of oscillation frequency content before and after PCP injection, n = 5 mice. b , For PCP, example temporal video sequence of 410 nm-corrected fluorescence over 1 s in a Thy1-GCaMP6s mouse showing RSP rhythm. c , Quantification of oscillation frequency content before and after MK801 injection, n = 5 mice. d , For MK801, example temporal video sequence of 410 nm-corrected fluorescence over 1 s in a Thy1-GCaMP6s mouse, showing RSP rhythm. e , Quantification of oscillation frequency content before and after memantine injection, n = 5 mice. f , For memantine, example temporal video sequence of 410 nm-corrected fluorescence across 1 s in a Thy1-GCaMP6s mouse. Here, neither rhythms nor travelling waves are seen. g , Quantification of oscillation frequency content before and after saline injection, n = 5 mice. h , Quantification of mean power in 1–3 Hz band for saline ( n = 5 mice), corrected two-sided paired t -tests (* P

    Journal: Nature

    Article Title: Deep posteromedial cortical rhythm in dissociation

    doi: 10.1038/s41586-020-2731-9

    Figure Lengend Snippet: PCP and MK801—but not memantine, saline or LSD—induce RSP-localized rhythms. a , Quantification of oscillation frequency content before and after PCP injection, n = 5 mice. b , For PCP, example temporal video sequence of 410 nm-corrected fluorescence over 1 s in a Thy1-GCaMP6s mouse showing RSP rhythm. c , Quantification of oscillation frequency content before and after MK801 injection, n = 5 mice. d , For MK801, example temporal video sequence of 410 nm-corrected fluorescence over 1 s in a Thy1-GCaMP6s mouse, showing RSP rhythm. e , Quantification of oscillation frequency content before and after memantine injection, n = 5 mice. f , For memantine, example temporal video sequence of 410 nm-corrected fluorescence across 1 s in a Thy1-GCaMP6s mouse. Here, neither rhythms nor travelling waves are seen. g , Quantification of oscillation frequency content before and after saline injection, n = 5 mice. h , Quantification of mean power in 1–3 Hz band for saline ( n = 5 mice), corrected two-sided paired t -tests (* P

    Article Snippet: Drugs used were ketamine (VEDCO, ketamine HCl, 6–200 mg kg−1 ), phencyclidine (PCP, 5 mg kg−1 , Sigma-Aldrich P3029), memantine (50 mg kg−1 ), MK801 (0.75–1 mg kg−1 ), dexmedetomidine (0.35–1.5 mg kg−1 ), propofol (35–140 mg kg−1 ), xylazine (AnaSed AKORN, 12.5 mg kg−1 ), buprenorphine SR (2 mg kg−1 ), lidocaine (topical), LSD ((+)-lysergic acid diethylamide (+)-tartrate (2:1), 0.3 mg kg−1 ) and diazepam (2 mg kg−1 ).

    Techniques: Injection, Mouse Assay, Sequencing, Fluorescence