drugs dizocilpine  (Alomone Labs)

Journal: Pharmaceuticals

Article Title: Administration of Bacterial Lipopolysaccharide during Early Postnatal Ontogenesis Induces Transient Impairment of Long-Term Synaptic Plasticity Associated with Behavioral Abnormalities in Young Rats

doi: 10.3390/ph13030048

Figure Lengend Snippet: LTP properties are not disturbed in adolescent rats administered with LPS during the first postnatal week (1wLPS_a) or third postnatal week (3wLPS_a). ( A ) Diagram showing the normalized slope of fEPSP in the control (ctrl) and experimental groups (1wLPS_a and 3wLPS_a) before and after TBS. ( B ) Diagram illustrating average LTP values in these groups. One-way ANOVA: F 2;48 = 0.27; p = 0.77. ( C ) Diagram showing the PPRs of fEPSPs before (baseline) and after (LTP) TBS in different groups. ( D ) Diagram illustrating the effect of MK-801 (10 μM) on LTP magnitude in the control (ctrl) and experimental (1wLPS_a; 3wLPS_a) groups * p

Article Snippet: Drugs Dizocilpine (MK-801, Alomone Labs, Israel, cat # M-230, 10 µM), a non-competitive antagonist of NMDAR, 4-[1-(2-fluoropyridin-3-yl)-5-methyltriazol-4-yl]-N-methylN-propan-2-yl-3,6dihydro-2H-pyridine-1-carboxamide (FTIDC, Alomone Labs, cat # F-190, 5 µM), a potent and selective antagonist of mGlu1Rs, and 4-[2-(4-Benzylpiperidin-1-yl)-1-hydroxypropyl]phenol (ifenprodil, Alomone Labs, cat # I-105, 3 µM), a NMDAR antagonist that selectively inhibits receptors containing the NR2B subunit, were used for the electrophysiology experiments and were diluted in distilled water and then bath-applied.

Techniques:

Journal: Pharmaceuticals

Article Title: Administration of Bacterial Lipopolysaccharide during Early Postnatal Ontogenesis Induces Transient Impairment of Long-Term Synaptic Plasticity Associated with Behavioral Abnormalities in Young Rats

doi: 10.3390/ph13030048

Figure Lengend Snippet: The NMDA receptor (NMDAR)-dependent mechanism of LTP induction is disrupted in the CA1 hippocampus of juvenile animals treated with LPS during the third postnatal week. ( A – C ) The normalized fEPSP slope in the control and experimental groups in the presence of the NMDAR blocker Dizocilpine (MK-801) (10 μM) before and after TBS. ( D – F ) The relative fEPSP slope in the control and experimental groups in the presence of ifenprodil (If, 3 μM), a selective GluN2B-containing NMDAR antagonist, before and after TBS. ( G – H ) Diagrams illustrating the magnitude of plasticity in the control and experimental groups in the presence of MK-801 or ifenprodil. Two-way ANOVA following Tukey post hoc tests were used. * p

Article Snippet: Drugs Dizocilpine (MK-801, Alomone Labs, Israel, cat # M-230, 10 µM), a non-competitive antagonist of NMDAR, 4-[1-(2-fluoropyridin-3-yl)-5-methyltriazol-4-yl]-N-methylN-propan-2-yl-3,6dihydro-2H-pyridine-1-carboxamide (FTIDC, Alomone Labs, cat # F-190, 5 µM), a potent and selective antagonist of mGlu1Rs, and 4-[2-(4-Benzylpiperidin-1-yl)-1-hydroxypropyl]phenol (ifenprodil, Alomone Labs, cat # I-105, 3 µM), a NMDAR antagonist that selectively inhibits receptors containing the NR2B subunit, were used for the electrophysiology experiments and were diluted in distilled water and then bath-applied.

Techniques: