dra i  (New England Biolabs)


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    Structured Review

    New England Biolabs dra i
    Dra I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dra i/product/New England Biolabs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dra i - by Bioz Stars, 2020-05
    93/100 stars

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    Clone Assay:

    Article Title: Synaptic silencing of fast muscle is compensated by rewired innervation of slow muscle
    Article Snippet: .. After cloning and digestion by Dra I (New England Biolabs), gRNA was transcribed by T7 polymerase (Roche, Molecular Biochemicals GmbH, Mannheim, Germany). .. The Cas9 mRNA was transcribed using Pme I–digested Cas9 expression vector (MLM 3613; Addgene) by an mMESSAGE mMACHINE T7 ULTRA kit (Thermo Fisher Scientific, Waltham, MA).

    other:

    Article Title: Occurrence of subdioecy and scarcity of gender-specific markers reveal an ongoing transition to dioecy in Himalayan seabuckthorn (Hippophae rhamnoides ssp. turkestanica)
    Article Snippet: Total genomic DNA (1 µg) was digested with rare DNA restriction enzymes, that is, Dra I, Ssp I, Stu I, and Eco RV (NEB, India).

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    New England Biolabs dra i
    Expression of the 46-bp insertion and detection of the two types of 16S rRNA by <t>RT-PCR.</t> The strains analyzed are indicated at the top. (A) Lanes +, use of consensual primer pair 27f and 590r; lanes ins, use of an insertion-specific pair of primers, ins1 and 590r. (B) Lanes +, RT-PCR products obtained with the primers 27f and 590r; lanes Dra I, RT-PCR products obtained in + lanes digested by Dra I. Lanes − (A and B), negative controls performed on the RNA of strain PR17/sat without reverse transcription and with the primers 27f and 590r (negative controls were also obtained for other strains and for the primers ins1 and 590r but are not shown); lane M, 100-bp DNA ladder (Promega) as a molecular weight marker.
    Dra I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dra i/product/New England Biolabs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dra i - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    88
    New England Biolabs restriction enzyme dra i
    Examples of genomic DNA macrorestriction profiles of S. maltophilia produced by PFGE after <t>Dra</t> I digestion. Lane 1, c12; lane 2, c1; lane 3, c7; lane 4, c17; lane 5, c5; lane 6, e1; lane 7, e2; lane 8, lambda ladder marker (size range, 225 to 1,900 kb); lane 9, e9; lane 10, e10; lane 11, t20; lane 12, e18; lane 13, e5.
    Restriction Enzyme Dra I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme dra i/product/New England Biolabs
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme dra i - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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    Expression of the 46-bp insertion and detection of the two types of 16S rRNA by RT-PCR. The strains analyzed are indicated at the top. (A) Lanes +, use of consensual primer pair 27f and 590r; lanes ins, use of an insertion-specific pair of primers, ins1 and 590r. (B) Lanes +, RT-PCR products obtained with the primers 27f and 590r; lanes Dra I, RT-PCR products obtained in + lanes digested by Dra I. Lanes − (A and B), negative controls performed on the RNA of strain PR17/sat without reverse transcription and with the primers 27f and 590r (negative controls were also obtained for other strains and for the primers ins1 and 590r but are not shown); lane M, 100-bp DNA ladder (Promega) as a molecular weight marker.

    Journal: Journal of Bacteriology

    Article Title: Atypical 16S rRNA Gene Copies in Ochrobactrum intermedium Strains Reveal a Large Genomic Rearrangement by Recombination between rrn Copies

    doi: 10.1128/JB.185.9.2901-2909.2003

    Figure Lengend Snippet: Expression of the 46-bp insertion and detection of the two types of 16S rRNA by RT-PCR. The strains analyzed are indicated at the top. (A) Lanes +, use of consensual primer pair 27f and 590r; lanes ins, use of an insertion-specific pair of primers, ins1 and 590r. (B) Lanes +, RT-PCR products obtained with the primers 27f and 590r; lanes Dra I, RT-PCR products obtained in + lanes digested by Dra I. Lanes − (A and B), negative controls performed on the RNA of strain PR17/sat without reverse transcription and with the primers 27f and 590r (negative controls were also obtained for other strains and for the primers ins1 and 590r but are not shown); lane M, 100-bp DNA ladder (Promega) as a molecular weight marker.

    Article Snippet: Restriction analysis of PCR products was done with 10 U of Dra I (New England Biolabs, Hertfordshire, United Kingdom) by following the supplier's recommendations. ) and with sequences deposited in the Ribosomal Database Project, version 7.0, by using SIMILARITY RANK and SUGGEST TREE ( ).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Marker

    Effect of an abasic site on DNA synthesis by reconstituted phage T4 replication complexes. ( A ) An abasic site blocks DNA synthesis by T4 DNA polymerase holoenzyme. 32 P-end-labeled 21mer #7 annealed with 120mer #3 (lanes 1, 3, 5, 7, 9, 11, 13 and 15) or with the same oligomer containing an abasic site at position 99 (120mer #4) (lanes 2, 4, 6, 8, 10, 12, 14 and 16) was incubated with either T4 gp43 (lanes 5, 6, 13 and 14) or the exonuclease-deficient gp43D219A (lanes 7, 8, 15 and 16) in the presence of gp44/62, gp45 and gp32 for the indicated times, and the products were separated in a 10% polyacrylamide gel + 6 M urea. Note that in the substrate with an abasic site, the first nucleotide to be incorporated into the 32 P-end-labeled 21mer is opposite the abasic site. ( B ) Block to lagging-strand synthesis imposed by the abasic site. Products of DNA synthesized by reconstituted T4 replication complexes in the presence of [α- 32 P]dCTP and 240mer circular ssDNA annealed with the 120mer ssDNA without (lanes 1 and 2) or with (lanes 3 and 4) abasic site were digested (lanes 2 and 4) or not (lanes 1 and 3) by Dra I and separated in a 6% polyacrylamide gel + 6 M urea along with marker DNA. Note that the 240mer circular ssDNA has a unique Dra I site. Upon Dra I cleavage of the replication products, the major 240mer fragment is formed along with other fragments that are located between the Dra I site and either the 3′ or 5′ ends of Okazaki fragments. Arrows show positions of the major 240-nt Dra I fragment and the 126-nt fragment located between the Dra I site and the 3′ end of the nascent fragment terminated by the abasic site. ( C ) DNA products were synthesized by reconstituted T4 replication complexes in the presence of [α- 32 P]dTTP and of 240mer circular ssDNA annealed with 120mer ssDNA without (lanes 1–3) or with (lanes 4–6) an abasic site, and separated in a 0.6% alkali gel.

    Journal: Nucleic Acids Research

    Article Title: Characterization of DNA synthesis catalyzed by bacteriophage T4 replication complexes reconstituted on synthetic circular substrates

    doi:

    Figure Lengend Snippet: Effect of an abasic site on DNA synthesis by reconstituted phage T4 replication complexes. ( A ) An abasic site blocks DNA synthesis by T4 DNA polymerase holoenzyme. 32 P-end-labeled 21mer #7 annealed with 120mer #3 (lanes 1, 3, 5, 7, 9, 11, 13 and 15) or with the same oligomer containing an abasic site at position 99 (120mer #4) (lanes 2, 4, 6, 8, 10, 12, 14 and 16) was incubated with either T4 gp43 (lanes 5, 6, 13 and 14) or the exonuclease-deficient gp43D219A (lanes 7, 8, 15 and 16) in the presence of gp44/62, gp45 and gp32 for the indicated times, and the products were separated in a 10% polyacrylamide gel + 6 M urea. Note that in the substrate with an abasic site, the first nucleotide to be incorporated into the 32 P-end-labeled 21mer is opposite the abasic site. ( B ) Block to lagging-strand synthesis imposed by the abasic site. Products of DNA synthesized by reconstituted T4 replication complexes in the presence of [α- 32 P]dCTP and 240mer circular ssDNA annealed with the 120mer ssDNA without (lanes 1 and 2) or with (lanes 3 and 4) abasic site were digested (lanes 2 and 4) or not (lanes 1 and 3) by Dra I and separated in a 6% polyacrylamide gel + 6 M urea along with marker DNA. Note that the 240mer circular ssDNA has a unique Dra I site. Upon Dra I cleavage of the replication products, the major 240mer fragment is formed along with other fragments that are located between the Dra I site and either the 3′ or 5′ ends of Okazaki fragments. Arrows show positions of the major 240-nt Dra I fragment and the 126-nt fragment located between the Dra I site and the 3′ end of the nascent fragment terminated by the abasic site. ( C ) DNA products were synthesized by reconstituted T4 replication complexes in the presence of [α- 32 P]dTTP and of 240mer circular ssDNA annealed with 120mer ssDNA without (lanes 1–3) or with (lanes 4–6) an abasic site, and separated in a 0.6% alkali gel.

    Article Snippet: T4 DNA ligase, Dra I and polynucleotide kinase were from New England Biolabs.

    Techniques: DNA Synthesis, Labeling, Incubation, Blocking Assay, Synthesized, Marker

    PFGE profiles of Sfi I-, Apa I-, Dra I-, Spe I-, and Xba I-digested whole-cell DNA of O. urethralis COH-1 (A; lanes 1 to 5) and their Southern transfer, followed by hybridization with internal probes specific for bla ABA-1 (B) and bla CARB-8 (C). Lanes M, molecular size markers

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Chromosomal Integration of a Cephalosporinase Gene from Acinetobacter baumannii into Oligella urethralis as a Source of Acquired Resistance to ?-Lactams

    doi: 10.1128/AAC.47.5.1536-1542.2003

    Figure Lengend Snippet: PFGE profiles of Sfi I-, Apa I-, Dra I-, Spe I-, and Xba I-digested whole-cell DNA of O. urethralis COH-1 (A; lanes 1 to 5) and their Southern transfer, followed by hybridization with internal probes specific for bla ABA-1 (B) and bla CARB-8 (C). Lanes M, molecular size markers

    Article Snippet: Macrorestriction analysis of whole-cell DNA of O. urethralis COH-1 was done by the pulsed-field gel electrophoresis (PFGE) technique with Xba I, Spe I, Dra I, Apa I, and Sfi I (New England Biolabs), as reported previously ( , ).

    Techniques: Hybridization

    Examples of genomic DNA macrorestriction profiles of S. maltophilia produced by PFGE after Dra I digestion. Lane 1, c12; lane 2, c1; lane 3, c7; lane 4, c17; lane 5, c5; lane 6, e1; lane 7, e2; lane 8, lambda ladder marker (size range, 225 to 1,900 kb); lane 9, e9; lane 10, e10; lane 11, t20; lane 12, e18; lane 13, e5.

    Journal: Journal of Clinical Microbiology

    Article Title: Genotypic and Phenotypic Relationships between Clinical and Environmental Isolates of Stenotrophomonas maltophilia

    doi:

    Figure Lengend Snippet: Examples of genomic DNA macrorestriction profiles of S. maltophilia produced by PFGE after Dra I digestion. Lane 1, c12; lane 2, c1; lane 3, c7; lane 4, c17; lane 5, c5; lane 6, e1; lane 7, e2; lane 8, lambda ladder marker (size range, 225 to 1,900 kb); lane 9, e9; lane 10, e10; lane 11, t20; lane 12, e18; lane 13, e5.

    Article Snippet: Agarose plugs were digested with restriction enzyme Dra I (New England Biolabs, Schalbach, Germany) for 20 h at 35°C according to the manufacturer's recommendations.

    Techniques: Produced, Marker