double strand circular φx174 rfi dna  (New England Biolabs)


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    Structured Review

    New England Biolabs double strand circular φx174 rfi dna
    Double Strand Circular φx174 Rfi Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/double strand circular φx174 rfi dna/product/New England Biolabs
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    double strand circular φx174 rfi dna - by Bioz Stars, 2020-08
    84/100 stars

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    In Vitro:

    Article Title: RADA is the main branch migration factor in plant mitochondrial recombination and its defect leads to mtDNA instability and cell cycle arrest
    Article Snippet: .. In vitro strand exchange reaction Recombination assays were performed with single-strand linear ΦX174 virion DNA and double strand circular ΦX174 RFI DNA (New England Biolabs) linearized with PstI in 20 mM Tris-acetate pH 7.4, 12.5 mM phosphocreatine, 10 U/mL creatine kinase, 3 mM ammonium glutamate, 1 mM dithiothreitol, 2 % glycerol and 11 mM magnesium acetate. .. In our conditions, 20.1 µM (in nucleotides) linear single strand DNA (ssDNA), 6.7 µM RecA (New England Biolabs), 2 µM RADA are incubated with buffer for 8 min at 37 °C.

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    New England Biolabs bacteriophage φx174 dna
    <t>DNA</t> strand exchange reaction. A scheme of the reaction (a), the temperature (b) and kinetic (c) characteristics, and control reactions (d) are shown. In panels b and c, the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized M13mp18 dsDNA. The latter was added to initiate the reaction after 5 min of preincubation at the temperature indicated. In panel d (homologous controls, lanes 1 to 5), the reaction mixture (20 μl) was the same as described above, but either without RadA Da (lane 1) or with RadA Da degraded by proteinase K (PrK) (lane 2); a complete mixture (lane 3); the same mixture as in lane 3, but without ATP (lane 4); or the same mixture as in lane 3, but without Mg +2 (lane 5). In panel d (heterologous controls, lanes 6 to 8), the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized <t>φX174</t> dsDNA (lane 6) or 8 μM φX174 ssDNA and 16 μM linearized M13mp18 dsDNA (lane 7), or 8 μM φX174 ssDNA and 16 μM linearized φX174 dsDNA (lane 8, showing the strand exchange reaction with φX174 DNA substrates).
    Bacteriophage φx174 Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteriophage φx174 dna/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacteriophage φx174 dna - by Bioz Stars, 2020-08
    80/100 stars
      Buy from Supplier

    84
    New England Biolabs double strand circular φx174 rfi dna
    <t>DNA</t> strand exchange reaction. A scheme of the reaction (a), the temperature (b) and kinetic (c) characteristics, and control reactions (d) are shown. In panels b and c, the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized M13mp18 dsDNA. The latter was added to initiate the reaction after 5 min of preincubation at the temperature indicated. In panel d (homologous controls, lanes 1 to 5), the reaction mixture (20 μl) was the same as described above, but either without RadA Da (lane 1) or with RadA Da degraded by proteinase K (PrK) (lane 2); a complete mixture (lane 3); the same mixture as in lane 3, but without ATP (lane 4); or the same mixture as in lane 3, but without Mg +2 (lane 5). In panel d (heterologous controls, lanes 6 to 8), the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized <t>φX174</t> dsDNA (lane 6) or 8 μM φX174 ssDNA and 16 μM linearized M13mp18 dsDNA (lane 7), or 8 μM φX174 ssDNA and 16 μM linearized φX174 dsDNA (lane 8, showing the strand exchange reaction with φX174 DNA substrates).
    Double Strand Circular φx174 Rfi Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/double strand circular φx174 rfi dna/product/New England Biolabs
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    double strand circular φx174 rfi dna - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

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    DNA strand exchange reaction. A scheme of the reaction (a), the temperature (b) and kinetic (c) characteristics, and control reactions (d) are shown. In panels b and c, the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized M13mp18 dsDNA. The latter was added to initiate the reaction after 5 min of preincubation at the temperature indicated. In panel d (homologous controls, lanes 1 to 5), the reaction mixture (20 μl) was the same as described above, but either without RadA Da (lane 1) or with RadA Da degraded by proteinase K (PrK) (lane 2); a complete mixture (lane 3); the same mixture as in lane 3, but without ATP (lane 4); or the same mixture as in lane 3, but without Mg +2 (lane 5). In panel d (heterologous controls, lanes 6 to 8), the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized φX174 dsDNA (lane 6) or 8 μM φX174 ssDNA and 16 μM linearized M13mp18 dsDNA (lane 7), or 8 μM φX174 ssDNA and 16 μM linearized φX174 dsDNA (lane 8, showing the strand exchange reaction with φX174 DNA substrates).

    Journal: Journal of Bacteriology

    Article Title: Efficient Strand Transfer by the RadA Recombinase from the Hyperthermophilic Archaeon Desulfurococcus amylolyticus

    doi:

    Figure Lengend Snippet: DNA strand exchange reaction. A scheme of the reaction (a), the temperature (b) and kinetic (c) characteristics, and control reactions (d) are shown. In panels b and c, the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized M13mp18 dsDNA. The latter was added to initiate the reaction after 5 min of preincubation at the temperature indicated. In panel d (homologous controls, lanes 1 to 5), the reaction mixture (20 μl) was the same as described above, but either without RadA Da (lane 1) or with RadA Da degraded by proteinase K (PrK) (lane 2); a complete mixture (lane 3); the same mixture as in lane 3, but without ATP (lane 4); or the same mixture as in lane 3, but without Mg +2 (lane 5). In panel d (heterologous controls, lanes 6 to 8), the reaction mixture (20 μl) contained TAcMD buffer (pH 7.9), 2.5 mM ATP, 8 μM RadA Da , 8 μM M13mp18 ssDNA, and 16 μM linearized φX174 dsDNA (lane 6) or 8 μM φX174 ssDNA and 16 μM linearized M13mp18 dsDNA (lane 7), or 8 μM φX174 ssDNA and 16 μM linearized φX174 dsDNA (lane 8, showing the strand exchange reaction with φX174 DNA substrates).

    Article Snippet: Bacteriophage φX174 DNA (RFI and circular ssDNA) was obtained from New England Biolabs.

    Techniques: