Journal: Nucleic Acids Research

Article Title: Molecular basis for SOX2-dependent regulation of super-enhancer activity

doi: 10.1093/nar/gkad908

Figure Lengend Snippet: Confocal microscopic analysis of enhancer activity of mSE078 fragments using PCP-PP7 system in MEF cells. ( A ) Left: Schematic representation of mkrn1 P -12 × PP7-SE reporter plasmid construct. Right: Schematic representation of PCP-PP7 system used in this study. ( B ) Confocal microscopic analysis of tdPCP-CFP (transcript), SOX2-mCherry and p300-GFP signals in MEF cells. Cells were imaged after 24 h-post transfections of four plasmids. Left: 40× magnification images; Right: closed-up images of single cells. ( C ) Quantification of CFP (PCP), mCherry (SOX2) and GFP (p300) foci. Error bars indicate standard errors of means (SEM) from at least 15 nuclei. Quantification was done using ImageJ software. Particle size larger than 2 × 2 μm 2 was calculated. ( D ) Pearson's coefficients of CFP and GFP signals to mCherry signals. Pearson's coefficients were calculated using ImageJ software. Error bars indicate SEMs from ten nuclei. ( E ) Fluorescence recovery after photobleaching (FRAP) measuring SOX2-mCherry condensates in MEF cells co-transfected with PCP-CFP, SOX2-mCherry and p300-GFP plasmids. Photobleaching was initiated at 0 s. Curve shows mean (red dot) and SEM (black bar) of mCherry intensity of five regions. FRAP recovery curves were fitted to the double exponential function. P -values were calculated using one-way ANOVA. (ns) P < 0.1234; (*) P < 0.0332; (**) P < 0.0021; (***) P < 0.0002; (****) P < 0.0001.

Article Snippet: FRAP recovery curves were fitted to the double exponential function in GraphPad Prism software v.8.3.0 (GraphPad software, Boston, MA, USA).

Techniques: Activity Assay, Plasmid Preparation, Construct, Transfection, Software, Fluorescence

Journal: Nanomaterials

Article Title: The Mechanical Properties of Blended Fibrinogen:Polycaprolactone (PCL) Nanofibers

doi: 10.3390/nano13081359

Figure Lengend Snippet: Incremental stress–strain curves. ( A ) Strain versus time curve for electrospun 75:25 fibrinogen:PCL fiber. The fiber was pulled to a small strain (~10%) and held constant for approximately 30–40 s; this process was repeated with a slightly larger strain at each time. ( B ) Stress versus time curve. At constant strain, the stress relaxes and decays exponentially with time. ( C ) Representative stress relaxation curves. A double exponential curve is fitted to the relaxation curve (R 2 = 0.99) to determine the relaxation times. The fast and slow relaxation times for this curve were 1.8 s and 21 s. ( D ) Moduli versus strain curve. The total modulus, Y tot , (stars) and relaxed, elastic modulus, Y 0 , (dots) decrease as the strain increases. ( E ) The graph shows statistical differences between the slow and fast relaxation times of the fibers with two different ratios. The fiber diameter was 99 nm. ** indicates a p -value < 0.01; **** indicates a p -value < 0.0001.

Article Snippet: Individual stress relaxation curves were fitted to this double exponential function in Origin (OriginLab Corporation, Northampton, MA, USA).

Techniques: