donkey antigoat igg  (Thermo Fisher)


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    Name:
    Donkey anti Goat IgG Secondary Antibody
    Description:
    Donkey anti Goat IgG Secondary Antibody for Western Blot IHC F IHC P
    Catalog Number:
    pa128659
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher donkey antigoat igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of <t>antigoat</t> <t>IgG</t> (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Donkey anti Goat IgG Secondary Antibody for Western Blot IHC F IHC P
    https://www.bioz.com/result/donkey antigoat igg/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey antigoat igg - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct"

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    Journal: Experimental Biology and Medicine

    doi: 10.1177/1535370216673746

    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Negative Control

    Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Cell Culture, Incubation, Negative Control

    2) Product Images from "Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct"

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    Journal: Experimental Biology and Medicine

    doi: 10.1177/1535370216673746

    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Negative Control

    Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)
    Figure Legend Snippet: Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Techniques Used: Fluorescence, Staining, Cell Culture, Incubation, Negative Control

    Related Articles

    Incubation:

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct
    Article Snippet: .. Following washing with PBS, the slices were incubated with secondary antibodies of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen), and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen), and goat antimouse IgG (Alexa Fluor® 594-conjugated; Invitrogen) for 30 min at room temperature in the humidified chamber. .. After nucleus counterstain and mounting, the slices were evaluated by a fluorescence microscope (Leica DM4000B).

    Article Title: Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation
    Article Snippet: .. After 3 hr incubation the membranes were washed three times 15 min with PBS + 0.1% Tween, followed by incubation with donkey anti-goat IgG alexa fluor 800 conjugate #W10825 Thermo Fischer (1/10000) and goat anti–rabbit IgG alexa fluor 680 conjugate #A27042 Thermo Fischer (1/10000) and revealed by ODYSSEY scanner device (Li-Cor Biosciences), respectively. ..

    Article Title: Effect and molecular mechanism of mTOR inhibitor rapamycin on temozolomide-induced autophagic death of U251 glioma cells
    Article Snippet: .. Secondary antibody [dilution: 10 µg/ml, donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 350, catalog no. A-21081, Thermo Fisher Scientific Inc.] was added to the cells cultured on glass coverslips and incubated at 37°C for 30 min. .. Cells were counterstained with DAPI and incubated at 37°C for a further 10 min.

    Article Title: bFGF attenuates endoplasmic reticulum stress and mitochondrial injury on myocardial ischaemia/reperfusion via activation of PI3K/Akt/ERK1/2 pathway
    Article Snippet: .. After primary antibody incubation, the sections were washed for 4 × 10 min. at room temperature and then incubated with donkey antimouse/rabbit, donkey antirabbit/mouse or donkey antigoat secondary antibody (1:500; Invitrogen) for 1 hr at room temperature. ..

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct
    Article Snippet: .. The cells were then incubated with a mixed solution of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen, Grand Island, NY) and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen) for 60 min in the dark. .. Next, a mounting medium for fluorescence with DAPI (Vectashield, Burlingame, CA) was used to counterstain the nucleus.

    Article Title: Prenatal Inflammation Dampens Neurogenesis and Enhances Serotonin Transporter Expression in the Hippocampus of Adult Female Rats
    Article Snippet: .. Brain sections were incubated with doublecortin (DCX) antibody made in goat (1:1,000; Santa Cruz Biotechnology, CA, USA) followed by Alexa Fluor 555 bound secondary antibody (donkey anti-goat IgG at 1:1,000; Life Technologies, CA, USA). .. DCX expression was used to monitor ongoing neurogenesis [ ].

    Article Title: Neuroprotective Activities of Heparin, Heparinase III, and Hyaluronic Acid on the Aβ42-Treated Forebrain Spheroids Derived from Human Stem Cells
    Article Snippet: .. After they were washed, the cells were incubated with the corresponding secondary antibody: Alexa Fluor 488 goat anti-Mouse IgG or Alexa Fluor 594 goat anti-Rabbit or donkey antigoat IgG (Life Technologies). .. The samples were stained with Hoechst 33342 and visualized using a fluorescent microscope.

    other:

    Article Title: Dysregulation of junctional adhesion molecule‐A contributes to ethanol‐induced barrier disruption in intestinal epithelial cell monolayers. Dysregulation of junctional adhesion molecule‐A contributes to ethanol‐induced barrier disruption in intestinal epithelial cell monolayers
    Article Snippet: Mouse monoclonal antioccludin, mouse monoclonal antizonula occludens‐2 (ZO‐2), and donkey polyclonal antigoat IgG (H+L)‐Alexa Fluor® 488 were purchased from Thermo Fisher (#33‐1500, #37‐4700, and #A‐11055).

    Cell Culture:

    Article Title: Effect and molecular mechanism of mTOR inhibitor rapamycin on temozolomide-induced autophagic death of U251 glioma cells
    Article Snippet: .. Secondary antibody [dilution: 10 µg/ml, donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 350, catalog no. A-21081, Thermo Fisher Scientific Inc.] was added to the cells cultured on glass coverslips and incubated at 37°C for 30 min. .. Cells were counterstained with DAPI and incubated at 37°C for a further 10 min.

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  • 90
    Thermo Fisher fitc labeled donkey antigoat igg
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Fitc Labeled Donkey Antigoat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc labeled donkey antigoat igg/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc labeled donkey antigoat igg - by Bioz Stars, 2020-07
    90/100 stars
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    85
    Thermo Fisher alexa fluor 594 donkey antigoat igg
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Alexa Fluor 594 Donkey Antigoat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 594 donkey antigoat igg/product/Thermo Fisher
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 594 donkey antigoat igg - by Bioz Stars, 2020-07
    85/100 stars
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    94
    Thermo Fisher donkey polyclonal antigoat igg
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Donkey Polyclonal Antigoat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey polyclonal antigoat igg/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey polyclonal antigoat igg - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor FITC labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.

    Journal: Veterinary World

    Article Title: Ocimum sanctum Linn. stimulate the expression of choline acetyltransferase on the human cerebral microvascular endothelial cells

    doi: 10.14202/vetworld.2016.1348-1354

    Figure Lengend Snippet: Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor FITC labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.

    Article Snippet: Labeled cells were visualized using FITC-labeled rabbit-antimouse IgG (Dako) or FITC-labeled donkey antigoat IgG (Thermo Scientific) and PE-labeled donkey antigoat IgG (Dako).

    Techniques: Expressing, Immunocytochemistry, Incubation, Labeling, Staining