donkey antigoat igg  (Jackson Immuno)

 
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    Name:
    Donkey Anti Goat IgG
    Description:
    Polyclonal antisera from immunized hosts are lipid extracted to improve clarity salt fractionated dialyzed against phosphate buffered saline containing sodium azide and freeze dried Antisera against whole serums are obtained by immunizing host animals with whole serum Antisera against whole IgG molecules i e Anti IgG H L are recommended for bridging PAP to primary antibodies Based on immunoelectrophoresis the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    705-001-003
    Price:
    36
    Purity:
    The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
    Conjugate:
    Unconjugated
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Donkey
    Quantity:
    2 0
    Buy from Supplier


    Structured Review

    Jackson Immuno donkey antigoat igg
    Donkey Anti Goat IgG
    Polyclonal antisera from immunized hosts are lipid extracted to improve clarity salt fractionated dialyzed against phosphate buffered saline containing sodium azide and freeze dried Antisera against whole serums are obtained by immunizing host animals with whole serum Antisera against whole IgG molecules i e Anti IgG H L are recommended for bridging PAP to primary antibodies Based on immunoelectrophoresis the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/donkey antigoat igg/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey antigoat igg - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Low oxygen tension enhances the generation of lung progenitor cells from mouse embryonic and induced pluripotent stem cells
    Article Snippet: .. After overnight primary antibody incubation, samples were washed with washing solution (0.1% BSA, 6% fetal bovine serum, 0.1% triton in TBS) and incubated with anti‐mouse (donkey)‐Alexa488, anti‐mouse (donkey)‐Dylight549, anti‐goat (donkey)‐Alexa488, anti‐mouse (donkey)‐Alexa647, anti‐rabbit (donkey)‐Cy3 secondary antibodies (all from Jackson Immunoresearch, Suffolk, UK). .. Samples were also counterstained with DAPI (#21490; Invitrogen, Carlsbad, CA).

    Article Title: Electrophysiological and Morphological Characterization of Propriospinal Interneurons in the Thoracic Spinal Cord
    Article Snippet: .. The selected sections were incubated at room temperature for 90 min in avidin conjugated to fluorescein isothiocyanate (Vector Laboratories, Peterborough, UK) and then for 3 days at 4°C in sheep antiserum against glutamic acid decarboxylase (GAD, diluted 1:600; a gift from Dr. W. H. Oertel) and monoclonal mouse antibody against gephyrin (mAb 7a, diluted 1:100; Boehringer Mannheim, Mannheim, Germany), and then overnight in species-specific secondary antibodies (donkey anti-goat immunoglobulin G [IgG] conjugated to Cy5 and donkey anti-mouse IgG conjugated to lissamine rhodamine, both diluted 1:100; both from Jackson ImmunoResearch, West Grove, PA). .. The sections were then scanned through a ×60 oil-immersion lens with a Bio-Rad MRC 1024 confocal microscope (Bio-Rad, Hemel Hempstead, UK), equipped with a krypton–argon laser.

    Article Title: Leptin Suppresses Mouse Taste Cell Responses to Sweet Compounds
    Article Snippet: .. Then, tissues were incubated with secondary antibodies for DIG (1:500, anti-DIG Fab fragments conjugated with alkaline phosphatase; Roche) and T1R3 (1:500, peroxidase-conjugated donkey anti-goat IgG; Jackson ImmunoResearch Laboratories, West Grove, PA) or GFP or GLAST (1:500, peroxidase-conjugated donkey anti-rabbit IgG; Jackson ImmunoResearch Laboratories); and then tyramide-Alexa Fluor 488 substrate (Molecular Probes TSA Kit; Life Technologies), AP buffer, and HNPP/FastRed AP substrate (HNPP Fluorescent Detection Kit; Roche). .. For triple staining with T1R3, SUR1, and Ob-Rb, the double Tyramide Signal Amplification labeling method ( ) was combined with ISH.

    other:

    Article Title: Cortical nNOS/NK1 Receptor Neurons are Regulated by Cholinergic Projections From the Basal Forebrain
    Article Snippet: One series of four sections from each mouse ( n = 3) was then processed with nDAB (10 min; SK4100, Vector Laboratories) to detect the BDA before secondary detection using goat anti-nNOS (1:3000, ab1376, abcam), followed by donkey antigoat IgG (1:1000, Jackson ImmunoResearch) and 3,3′ diaminobenzidine tetrahydrochloride (2 min; SK4100, Vector Laboratories).

    Immunohistochemistry:

    Article Title: VGluT3+ Primary Afferents Play Distinct Roles in Mechanical and Cold Hypersensitivity Depending on Pain Etiology
    Article Snippet: .. Immunohistochemistry for TH and eYFP (goat anti-GFP, 1:500, antikoerperonline.de; donkey anti-goat IgG Cy2, 1:400, Jackson ImmunoReseach) was performed subsequently, according to the above-mentioned protocol. ..

    Avidin-Biotin Assay:

    Article Title: Electrophysiological and Morphological Characterization of Propriospinal Interneurons in the Thoracic Spinal Cord
    Article Snippet: .. The selected sections were incubated at room temperature for 90 min in avidin conjugated to fluorescein isothiocyanate (Vector Laboratories, Peterborough, UK) and then for 3 days at 4°C in sheep antiserum against glutamic acid decarboxylase (GAD, diluted 1:600; a gift from Dr. W. H. Oertel) and monoclonal mouse antibody against gephyrin (mAb 7a, diluted 1:100; Boehringer Mannheim, Mannheim, Germany), and then overnight in species-specific secondary antibodies (donkey anti-goat immunoglobulin G [IgG] conjugated to Cy5 and donkey anti-mouse IgG conjugated to lissamine rhodamine, both diluted 1:100; both from Jackson ImmunoResearch, West Grove, PA). .. The sections were then scanned through a ×60 oil-immersion lens with a Bio-Rad MRC 1024 confocal microscope (Bio-Rad, Hemel Hempstead, UK), equipped with a krypton–argon laser.

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  • 99
    Jackson Immuno cy5 conjugated donkey antigoat igg
    Double immunolabeling in the C57BL/6J mouse. Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and retinal pigment epithelium-specific protein 65 kDa (RPE65) in sections from the posterior pole of a C57BL/6J mouse by laser scanning cytometry. Panel A shows RPE65 immunolabeling in the green channel (6 μg/ml mouse antibovine RPE65 monoclonal antibody; 7 μg/ml Alexa Fluor 488-conjugated donkey antimouse <t>IgG).</t> Panel B shows the same section viewed in the long-red channel. Panel C shows data from a MnSOD + /RPE65 + double-immunolabeled section in the green channel. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows the same section in the long-red channel (3 μg/ml goat anti-SOD2 antibody; 40 μg/ml Alexa Fluor 647-conjugated donkey <t>antigoat</t> IgG secondary antibody). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD+ represents cells expressing Mn-superoxide dismutase, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, C57BL/6J represents C57BL/6J strain of mice.
    Cy5 Conjugated Donkey Antigoat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy5 conjugated donkey antigoat igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy5 conjugated donkey antigoat igg - by Bioz Stars, 2020-07
    99/100 stars
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    85
    Jackson Immuno donkey antigoat biotinylated igg
    Double immunolabeling in the C57BL/6J mouse. Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and retinal pigment epithelium-specific protein 65 kDa (RPE65) in sections from the posterior pole of a C57BL/6J mouse by laser scanning cytometry. Panel A shows RPE65 immunolabeling in the green channel (6 μg/ml mouse antibovine RPE65 monoclonal antibody; 7 μg/ml Alexa Fluor 488-conjugated donkey antimouse <t>IgG).</t> Panel B shows the same section viewed in the long-red channel. Panel C shows data from a MnSOD + /RPE65 + double-immunolabeled section in the green channel. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows the same section in the long-red channel (3 μg/ml goat anti-SOD2 antibody; 40 μg/ml Alexa Fluor 647-conjugated donkey <t>antigoat</t> IgG secondary antibody). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD+ represents cells expressing Mn-superoxide dismutase, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, C57BL/6J represents C57BL/6J strain of mice.
    Donkey Antigoat Biotinylated Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey antigoat biotinylated igg/product/Jackson Immuno
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey antigoat biotinylated igg - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    96
    Jackson Immuno fitc
    Double immunolabeling in the C57BL/6J mouse. Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and retinal pigment epithelium-specific protein 65 kDa (RPE65) in sections from the posterior pole of a C57BL/6J mouse by laser scanning cytometry. Panel A shows RPE65 immunolabeling in the green channel (6 μg/ml mouse antibovine RPE65 monoclonal antibody; 7 μg/ml Alexa Fluor 488-conjugated donkey antimouse <t>IgG).</t> Panel B shows the same section viewed in the long-red channel. Panel C shows data from a MnSOD + /RPE65 + double-immunolabeled section in the green channel. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows the same section in the long-red channel (3 μg/ml goat anti-SOD2 antibody; 40 μg/ml Alexa Fluor 647-conjugated donkey <t>antigoat</t> IgG secondary antibody). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD+ represents cells expressing Mn-superoxide dismutase, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, C57BL/6J represents C57BL/6J strain of mice.
    Fitc, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1040 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc/product/Jackson Immuno
    Average 96 stars, based on 1040 article reviews
    Price from $9.99 to $1999.99
    fitc - by Bioz Stars, 2020-07
    96/100 stars
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    94
    Jackson Immuno donkey antigoat igg
    Double immunolabeling in the C57BL/6J mouse. Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and retinal pigment epithelium-specific protein 65 kDa (RPE65) in sections from the posterior pole of a C57BL/6J mouse by laser scanning cytometry. Panel A shows RPE65 immunolabeling in the green channel (6 μg/ml mouse antibovine RPE65 monoclonal antibody; 7 μg/ml Alexa Fluor 488-conjugated donkey antimouse <t>IgG).</t> Panel B shows the same section viewed in the long-red channel. Panel C shows data from a MnSOD + /RPE65 + double-immunolabeled section in the green channel. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows the same section in the long-red channel (3 μg/ml goat anti-SOD2 antibody; 40 μg/ml Alexa Fluor 647-conjugated donkey <t>antigoat</t> IgG secondary antibody). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD+ represents cells expressing Mn-superoxide dismutase, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, C57BL/6J represents C57BL/6J strain of mice.
    Donkey Antigoat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey antigoat igg/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey antigoat igg - by Bioz Stars, 2020-07
    94/100 stars
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    Double immunolabeling in the C57BL/6J mouse. Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and retinal pigment epithelium-specific protein 65 kDa (RPE65) in sections from the posterior pole of a C57BL/6J mouse by laser scanning cytometry. Panel A shows RPE65 immunolabeling in the green channel (6 μg/ml mouse antibovine RPE65 monoclonal antibody; 7 μg/ml Alexa Fluor 488-conjugated donkey antimouse IgG). Panel B shows the same section viewed in the long-red channel. Panel C shows data from a MnSOD + /RPE65 + double-immunolabeled section in the green channel. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows the same section in the long-red channel (3 μg/ml goat anti-SOD2 antibody; 40 μg/ml Alexa Fluor 647-conjugated donkey antigoat IgG secondary antibody). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD+ represents cells expressing Mn-superoxide dismutase, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, C57BL/6J represents C57BL/6J strain of mice.

    Journal: Molecular Vision

    Article Title: Quantification of retinal pigment epithelial phenotypic variation using laser scanning cytometry

    doi:

    Figure Lengend Snippet: Double immunolabeling in the C57BL/6J mouse. Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and retinal pigment epithelium-specific protein 65 kDa (RPE65) in sections from the posterior pole of a C57BL/6J mouse by laser scanning cytometry. Panel A shows RPE65 immunolabeling in the green channel (6 μg/ml mouse antibovine RPE65 monoclonal antibody; 7 μg/ml Alexa Fluor 488-conjugated donkey antimouse IgG). Panel B shows the same section viewed in the long-red channel. Panel C shows data from a MnSOD + /RPE65 + double-immunolabeled section in the green channel. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows the same section in the long-red channel (3 μg/ml goat anti-SOD2 antibody; 40 μg/ml Alexa Fluor 647-conjugated donkey antigoat IgG secondary antibody). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD+ represents cells expressing Mn-superoxide dismutase, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, C57BL/6J represents C57BL/6J strain of mice.

    Article Snippet: Slides were next incubated at 37 °C in a humidified chamber for 2 h with a mixture of secondary antibodies: 7.5 µg/ml Cy5-conjugated donkey antigoat IgG (Jackson ImmunoResearch, West Grove, PA) and 4 µg/ml of Alexa Fluor 488-conjugated donkey antimouse IgG (Molecular Probes, Eugene, OR) and 100 nM 4',6-diamidino-2-phenylindole (DAPI; Molecular Probes) diluted in immunofluorescence-labeling buffer.

    Techniques: Immunolabeling, Cytometry, Expressing, Mouse Assay

    Double immunolabeling for young and old retinal pigment epithelium (RPE). Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase (MnSOD) and retinal pigment epithelium-specific protein 65 kDa (RPE65) proteins in sections of human eyes at 14 months and 64 years by laser scanning cytometry. Formalin-fixed paraffin-embedded sections were prepared and processed for laser scanning cytometry from a 14-month-old male and a 64-year-old female eye. Panel A shows data for the 14-month-old eye double immunolabeled for MnSOD and RPE65 (6 μg/ml mouse monoclonal antibovine RPE65, 4 μg/ml goat anti-SOD2 primary antibodies and 4 μg/ml Alexa Fluor 488-conjugated donkey antimouse IgG and 40 μg/ml Alexa Fluor 647-conjugated donkey antigoat IgG secondary antibodies). Panel B shows a similar analysis of a section from a 64-year-old female eye.

    Journal: Molecular Vision

    Article Title: Quantification of retinal pigment epithelial phenotypic variation using laser scanning cytometry

    doi:

    Figure Lengend Snippet: Double immunolabeling for young and old retinal pigment epithelium (RPE). Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase (MnSOD) and retinal pigment epithelium-specific protein 65 kDa (RPE65) proteins in sections of human eyes at 14 months and 64 years by laser scanning cytometry. Formalin-fixed paraffin-embedded sections were prepared and processed for laser scanning cytometry from a 14-month-old male and a 64-year-old female eye. Panel A shows data for the 14-month-old eye double immunolabeled for MnSOD and RPE65 (6 μg/ml mouse monoclonal antibovine RPE65, 4 μg/ml goat anti-SOD2 primary antibodies and 4 μg/ml Alexa Fluor 488-conjugated donkey antimouse IgG and 40 μg/ml Alexa Fluor 647-conjugated donkey antigoat IgG secondary antibodies). Panel B shows a similar analysis of a section from a 64-year-old female eye.

    Article Snippet: Slides were next incubated at 37 °C in a humidified chamber for 2 h with a mixture of secondary antibodies: 7.5 µg/ml Cy5-conjugated donkey antigoat IgG (Jackson ImmunoResearch, West Grove, PA) and 4 µg/ml of Alexa Fluor 488-conjugated donkey antimouse IgG (Molecular Probes, Eugene, OR) and 100 nM 4',6-diamidino-2-phenylindole (DAPI; Molecular Probes) diluted in immunofluorescence-labeling buffer.

    Techniques: Immunolabeling, Cytometry, Formalin-fixed Paraffin-Embedded

    Immunolabeling for Mn-superoxide dismutase (MnSOD) in human retinal pigment epithelium (RPE). Panel A shows a negative control section. Panel B shows a section from the macula of a 64-year-old female; the section was processed for immunohistochemistry with 4 μg/ml goat antibody against Mn-superoxide dismutase protein (anti-SOD2) primary antibody, 7.5 μg/ml biotinylated rabbit antigoat IgG, avidin/biotin complexed with alkaline phosphatase, and NBT/BCIP substrate. Arrow heads point to cone photoreceptors, and arrows point to RPE cells. The magnification bar represents 10 µ (400×). Abbreviations: NBT represents 4-Nitro blue tetrazolium, BCIP represents 5-bromo-4-chloro-3-indolyl-phosphate.

    Journal: Molecular Vision

    Article Title: Quantification of retinal pigment epithelial phenotypic variation using laser scanning cytometry

    doi:

    Figure Lengend Snippet: Immunolabeling for Mn-superoxide dismutase (MnSOD) in human retinal pigment epithelium (RPE). Panel A shows a negative control section. Panel B shows a section from the macula of a 64-year-old female; the section was processed for immunohistochemistry with 4 μg/ml goat antibody against Mn-superoxide dismutase protein (anti-SOD2) primary antibody, 7.5 μg/ml biotinylated rabbit antigoat IgG, avidin/biotin complexed with alkaline phosphatase, and NBT/BCIP substrate. Arrow heads point to cone photoreceptors, and arrows point to RPE cells. The magnification bar represents 10 µ (400×). Abbreviations: NBT represents 4-Nitro blue tetrazolium, BCIP represents 5-bromo-4-chloro-3-indolyl-phosphate.

    Article Snippet: Slides were next incubated at 37 °C in a humidified chamber for 2 h with a mixture of secondary antibodies: 7.5 µg/ml Cy5-conjugated donkey antigoat IgG (Jackson ImmunoResearch, West Grove, PA) and 4 µg/ml of Alexa Fluor 488-conjugated donkey antimouse IgG (Molecular Probes, Eugene, OR) and 100 nM 4',6-diamidino-2-phenylindole (DAPI; Molecular Probes) diluted in immunofluorescence-labeling buffer.

    Techniques: Immunolabeling, Negative Control, Immunohistochemistry, Avidin-Biotin Assay

    Double immunolabeling for retinal pigment epithelium-specific protein 65 kDa (RPE65) and Mn-superoxide dismutase (MnSOD). Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and RPE65 in sections from the posterior pole of a 6- and 24-month-old BALB/c mouse by laser scanning cytometry. Panel A shows retinal pigment epithelium RPE65 + cells for the 6-month-old animal. Panel B shows Mn-superoxide dismutase MnSOD + /RPE65 + double-immunopositive cells for a 6-month-old animal. Panel C shows RPE65 + cells from a 24-month-old animal. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows MnSOD + /RPE65 + double-immunopositive cells. In each panel, fluorescence intensities are divided between two regions (1 and 2) defined by a gate at the indicated fluorescence intensity. Each panel shows statistics, including cell counts in each region (Count), total cell count (Total), percentage of cells in each region (Pct.), Mean (MFI), and coefficient of variation (CV). (Primary antibodies: 6 μg/ml mouse monoclonal antibovine RPE65 and 3 μg/ml goat anti-SOD2; Secondary antibodies: 4 μg/ml Alexa Fluor 488-conjugated donkey antimouse IgG and 40 μg/ml Alexa Fluor 647-conjugated donkey antigoat IgG). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, BALB/c represents BALB/c strain of mice.

    Journal: Molecular Vision

    Article Title: Quantification of retinal pigment epithelial phenotypic variation using laser scanning cytometry

    doi:

    Figure Lengend Snippet: Double immunolabeling for retinal pigment epithelium-specific protein 65 kDa (RPE65) and Mn-superoxide dismutase (MnSOD). Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase and RPE65 in sections from the posterior pole of a 6- and 24-month-old BALB/c mouse by laser scanning cytometry. Panel A shows retinal pigment epithelium RPE65 + cells for the 6-month-old animal. Panel B shows Mn-superoxide dismutase MnSOD + /RPE65 + double-immunopositive cells for a 6-month-old animal. Panel C shows RPE65 + cells from a 24-month-old animal. The red color in region 2 represents RPE cells that are RPE65 + MnSOD + , while green represents RPE cells that are RPE65 + MnSOD - . Panel D shows MnSOD + /RPE65 + double-immunopositive cells. In each panel, fluorescence intensities are divided between two regions (1 and 2) defined by a gate at the indicated fluorescence intensity. Each panel shows statistics, including cell counts in each region (Count), total cell count (Total), percentage of cells in each region (Pct.), Mean (MFI), and coefficient of variation (CV). (Primary antibodies: 6 μg/ml mouse monoclonal antibovine RPE65 and 3 μg/ml goat anti-SOD2; Secondary antibodies: 4 μg/ml Alexa Fluor 488-conjugated donkey antimouse IgG and 40 μg/ml Alexa Fluor 647-conjugated donkey antigoat IgG). Abbreviations: RPE65 + represents cells expressing RPE65 protein, MnSOD + /RPE65 + represents cells expressing both Mn-superoxide dismutase and RPE65 protein, BALB/c represents BALB/c strain of mice.

    Article Snippet: Slides were next incubated at 37 °C in a humidified chamber for 2 h with a mixture of secondary antibodies: 7.5 µg/ml Cy5-conjugated donkey antigoat IgG (Jackson ImmunoResearch, West Grove, PA) and 4 µg/ml of Alexa Fluor 488-conjugated donkey antimouse IgG (Molecular Probes, Eugene, OR) and 100 nM 4',6-diamidino-2-phenylindole (DAPI; Molecular Probes) diluted in immunofluorescence-labeling buffer.

    Techniques: Immunolabeling, Cytometry, Fluorescence, Cell Counting, Expressing, Mouse Assay