donkey antigoat igg irdye 800cw  (LI-COR)


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  • 98
    Name:
    IRDye 800CW Donkey anti Goat IgG H L
    Description:
    Highly cross adsorbed secondary antibody Suitable for a variety of applications including Western blotting In Cell Western Assays In Gel Westerns and many others
    Catalog Number:
    925-32214
    Price:
    None
    Applications:
    Western blot, In-Cell Western assay, In-Gel Westerns, in vivo imaging
    Conjugate:
    IRDye 800CW
    Size:
    1
    Category:
    IRDye secondary antibody
    Quantity:
    1
    Buy from Supplier


    Structured Review

    LI-COR donkey antigoat igg irdye 800cw
    IRDye 800CW Donkey anti Goat IgG H L
    Highly cross adsorbed secondary antibody Suitable for a variety of applications including Western blotting In Cell Western Assays In Gel Westerns and many others
    https://www.bioz.com/result/donkey antigoat igg irdye 800cw/product/LI-COR
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey antigoat igg irdye 800cw - by Bioz Stars, 2020-07
    98/100 stars

    Related Products / Commonly Used Together

    goat antirabbit igg ir680

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    Related Articles

    Western Blot:

    Article Title: Targeting PFKFB3 radiosensitizes cancer cells and suppresses homologous recombination
    Article Snippet: .. The secondary antibodies for Western blot were: donkey anti-mouse IgG IRDye 680RD (925-68072, 1:5000), goat anti-rabbit IgG IRDye 800CW (925-32211, 1:5000), donkey anti-rabbit IRDye 800CW (926-32213, 1:5000), donkey anti-mouse IRDye 800CW (926-32212, 1:5000), and donkey anti-goat IRDye 800CW (925-32214, 1:5000) from Li-Cor Biosciences. ..

    Article Title: A Human/Murine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro
    Article Snippet: .. Individual clones were identified by Western blotting using goat antihuman IgG (Fab-specific) and IRDye 800CW donkey antigoat IgG in an Odyssey infrared image system (LI-COR Biosciences, Lincoln, NE). .. Soluble LF8-Fab in the cell lysate was purified by immobilized metal affinity chromatography (IMAC) using a 5 mL His-trap HP column, followed by ion exchange chromatography (IEC) using a 5 mL Q sepharose column as described previously [ ].

    Incubation:

    Article Title: Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro
    Article Snippet: .. Membranes were then incubated with the primary antibodies, anti-oxytocin receptor rabbit antibody (1/10,000, ab181077, Abcam), anti-GABAA R α1 subunit rabbit antibody (1/3,000, ab3299, Abcam), anti-GABAA R α2 subunit rabbit antibody (1/1,000, ab72445, Abcam), anti-GABAA R α3 subunit rabbit antibody (1/1,000, ab72446, Abcam), anti-GABAA R α4 subunit rabbit antibody (1/1,000, ab4120, Abcam), anti-GABAA R α5 subunit rabbit antibody (1/3,000, ab10098, Abcam), anti-GABAA R α6 subunit goat antibody (1/1,000, ab117100, Abcam), anti-GABAA R β1 subunit rabbit antibody (1/3,000, ab154822, Abcam), anti-GABAA R β2 subunit rabbit antibody (1/30,000, ab16213, Abcam), anti-GABAA R β3 subunit rabbit antibody (1/1,000, ab4046, Abcam), anti-GABAA R γ1 subunit rabbit antibody (1/5,000, AMIN485542, Antibodies), anti-GABAA R γ2 subunit rabbit antibody (1/1,000, ab16213, Abcam), anti-GABAA R γ3 subunit rabbit antibody (1/500, ab13861, Abcam), anti-GABAA R δ subunit rabbit antibody (1/1,000, ab11048, Abcam), anti-GABAA R ε subunit rabbit antibody (1/500, ab35971, Abcam), anti-GABAA R π subunit rabbit antibody (1/5,000, ab26055, Abcam), anti-GABAA R θ subunit rabbit antibody (1/5,000, ARP5283, Antibodies), anti-bestrophin-1 (BST1) mouse antibody (1/3,000, NB300-164, Antibodies), anti-G6PD rabbit antibody (1/10,000, ab993, Abcam), and anti-GAPDH mouse antibody (1/10,000, ab8245, Abcam) at 4 °C for 14 h. After washing with PBS containing 0.1% Tween-20 (PBST), the nitrocellulose membrane was incubated with donkey anti-mouse IRDye800® CW secondary antibody (1/5,000, 926-32212, LI-COR, Lincoln, NE, USA), or donkey anti-rabbit IRDye800® CW secondary antibody (1/5,000, 926-32213, LI-COR), or donkey anti-goat IRDye800® CW secondary antibody (1/5,000, 926-32214, LI-COR) for 90 min at RT in dark. .. Immunoreactive detection using near-infrared fluorescence was performed according to the protocol of Odyssey® Infrared Imaging System (LI-COR® ).

    Article Title: Simulated microgravity hampers Notch signaling in the fight against myocardial ischemia-reperfusion injury
    Article Snippet: .. The membranes were incubated with IRDye 800CW-conjugated donkey-anti goat (1:10,000; cat. no. 926-32214; LI-COR, Inc.) or IRDye 800CW-conjugated goat-anti mouse secondary antibodies (1:10,000; cat. no. 926-32210; LI-COR, Inc.) for 90 min at room temperature, and were visualized using an Odyssey scanner (LI-COR Biosciences, Lincoln, NE, USA). .. Quantification analysis of blots was performed with the ImageJ software v1.43u (National Institutes of Health, Bethesda, MD, USA).

    other:

    Article Title: S-Glutathionylation of estrogen receptor α affects dendritic cell function
    Article Snippet: The following antibodies were used for immunoblotting: rabbit monoclonal anti-ERα (Millipore, Burlington, MA; 04-820, Lot 2135146; 1:500 dilution); rabbit polyclonal anti-GSTP (MBL International, Woburn, MA; 311, Lot 071; 1:1000 dilution); mouse monoclonal anti-GSH (Virogen, Watertown, MA; 101-A, Lot GS-D8/G2-68; 1:1000 dilution); rabbit polyclonal anti-β-actin (ab8227, Lot GR60359-1), rabbit polyclonal anti-glucose transporter GLUT1 (ab32551, Lot GR208248-1), and goat polyclonal anti-GSTP (ab53943, Lot GR121458-9) (all from Abcam, Cambridge, MA; 1:1000 dilution); rabbit polyclonal anti-Akt (9272, Lot 24), rabbit polyclonal anti-phospho-Akt (Ser-473; 9271, Lot 22), mouse monoclonal anti-AMPK (2793, Lot 4), and rabbit polyclonal anti-phospho-AMPK (Thr-172; 2535, Lot 16) (all from Cell Signaling Technology, Danvers, MA; 1:500 dilution); mouse monoclonal anti-PGAM1 (sc-130334, Lot G0317) and mouse monoclonal anti-β-enolase (sc-10081, Lot G2417) (both from Santa Cruz Biotechnology, Dallas, TX; 1:200 dilution); and IRDye 800CW goat anti-mouse IgG (926-32210, Lot C20510-05), IRDye 800CW goat anti-rabbit IgG (926-32211, Lot C21227-02), 800CW donkey anti-goat IgG (926-32214, Lot C60119-05), IRDye 680RD goat anti-mouse IgG (926-68070, Lot C70427-05), IRDye 680RD goat anti-rabbit IgG (926-68071, Lot C20530-01), and IRDye 680RD donkey anti-goat IgG (926-68074, Lot C20606-04) (all from LI-COR Biosciences, Lincoln, NE; 1:15,000 dilution).

    Article Title: Epigallocatechin-3-gallate, a prototypic chemopreventative agent for protection against cisplatin-based ototoxicity
    Article Snippet: Secondary antibodies used were as follows: donkey anti-rabbit IRDye 680RD (no. 926-68073), donkey anti-goat IRDye 800RD (no. 926-32212), goat anti-mouse IRDye 800RD (no. 926-32214) from LI-COR Biosciences (Lincoln, NE, USA); Alexa Fluor 488 goat anti-rabbit (no. A11008) from Thermo Fisher Scientific; Alexa Fluor 647 goat anti-mouse IgG1 (no. A-21240) from Molecular Probes (Eugene, OR, USA).

    Imaging:

    Article Title: Astrocytic modulation of excitatory synaptic signaling in a mouse model of Rett syndrome
    Article Snippet: .. Secondary antibodies were conjugated to IR-fluorophore for imaging on Li-Cor Odyssey machine: goat anti mouse- IR680 (Thermo Fisher 35518, Waltham, MA), goat anti rabit-IR680 (Thermo Fisher #33568, Waltham, MA) donkey anti goat- IR 800 (Li-Cor 925–32214, Lincoln, NE). ..

    Clone Assay:

    Article Title: A Human/Murine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro
    Article Snippet: .. Individual clones were identified by Western blotting using goat antihuman IgG (Fab-specific) and IRDye 800CW donkey antigoat IgG in an Odyssey infrared image system (LI-COR Biosciences, Lincoln, NE). .. Soluble LF8-Fab in the cell lysate was purified by immobilized metal affinity chromatography (IMAC) using a 5 mL His-trap HP column, followed by ion exchange chromatography (IEC) using a 5 mL Q sepharose column as described previously [ ].

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  • News
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  • Bioz Stars
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  • 98
    LI-COR irdye 800cw donkey antigoat igg
    Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and <t>IRDye</t> <t>800CW</t> donkey <t>antigoat</t> <t>IgG</t> were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.
    Irdye 800cw Donkey Antigoat Igg, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irdye 800cw donkey antigoat igg/product/LI-COR
    Average 98 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    irdye 800cw donkey antigoat igg - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

    99
    LI-COR irdye 800cw donkey anti rabbit igg h l
    Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and <t>IRDye</t> <t>800CW</t> donkey <t>antigoat</t> <t>IgG</t> were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.
    Irdye 800cw Donkey Anti Rabbit Igg H L, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irdye 800cw donkey anti rabbit igg h l/product/LI-COR
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    irdye 800cw donkey anti rabbit igg h l - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and IRDye 800CW donkey antigoat IgG were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.

    Journal: Clinical and Developmental Immunology

    Article Title: A Human/Murine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro

    doi: 10.1155/2013/475809

    Figure Lengend Snippet: Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and IRDye 800CW donkey antigoat IgG were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.

    Article Snippet: Individual clones were identified by Western blotting using goat antihuman IgG (Fab-specific) and IRDye 800CW donkey antigoat IgG in an Odyssey infrared image system (LI-COR Biosciences, Lincoln, NE).

    Techniques: Expressing, Purification, Western Blot, Sonication, Negative Control, Marker, Staining