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The Jackson Laboratory donkey anti goat igg
Donkey Anti Goat Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/donkey anti goat igg/product/The Jackson Laboratory
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
donkey anti goat igg - by Bioz Stars, 2020-07
92/100 stars

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Incubation:

Article Title: Transduction of Nonhuman Primate Brain with Adeno-Associated Virus Serotype 1: Vector Trafficking and Immune Response
Article Snippet: .. The next day, sections were washed in PBS with 2% goat serum and 0.01% Triton-X (three times, 5 min each), and incubated in secondary antibody for 1 hr (tetramethylrhodamine isothiocyanate [TRITC]-conjugated goat anti-mouse, goat anti-rabbit, or donkey anti-goat IgG [diluted 1:100; Jackson Laboratory, Bar Harbor, ME]). .. After three washes in PBS with 0.01% Triton-X, sections were mounted on slides, coverslipped, and examined by fluorescence microscopy.

other:

Article Title: Splicing factor hnRNP A2 activates the Ras-MAPK-ERK pathway by controlling A-Raf splicing in hepatocellular carcinoma development
Article Snippet: Secondary antibodies are as follows: HRP-conjugated goat anti-mouse, goat anti-rabbit, donkey anti-goat IgG (H+L; 1:10,000 Jackson Laboratories).

Article Title: Distribution of cholinergic contacts on Renshaw cells in the rat spinal cord: a light microscopic study
Article Snippet: Immunoreactive sites were revealed with donkey anti-mouse IgG coupled to Cy3 and donkey anti-goat IgG coupled to fluorescein isothiocyanate (FITC; Jackson Labs, West Grove, PA, USA; dilution 1:25 in PBS-TX) or tetramethylrhodamine isothiocyanate (TRITC; Jackson Labs; dilution 1:25 in PBS-TX).

Article Title: 2-APB and CBD-Mediated Targeting of Charged Cytotoxic Compounds Into Tumor Cells Suggests the Involvement of TRPV2 Channels
Article Snippet: Secondary antibodies used were as follows: HRP-conjugated goat anti-mouse, goat anti-rabbit, and donkey anti-goat IgG (1:10,000 Jackson Laboratories).

Article Title: The T3SS Effector EspT Defines a New Category of Invasive Enteropathogenic E. coli (EPEC) Which Form Intracellular Actin Pedestals
Article Snippet: Donkey anti-rabbit IgG conjugated to a Cy2 or Cy3 fluorophore, donkey anti-mouse IgG conjugated to a Cy5 or Cy5 fluorophore, donkey anti goat IgG conjugated to a Cy2 or Cy3 fluorophore (Jackson laboratories) were used at a 1∶200.

Article Title: RoXaN, a Novel Cellular Protein Containing TPR, LD, and Zinc Finger Motifs, Forms a Ternary Complex with Eukaryotic Initiation Factor 4G and Rotavirus NSP3
Article Snippet: Secondary antibodies goat anti-mouse immunoglobulin G (IgG), donkey anti-goat IgG (Jackson Laboratory), and goat anti-rabbit IgG (Sigma) conjugated to horseradish peroxidase were used as described below.

Article Title: Insulin receptor alternative splicing is regulated by insulin signaling and modulates beta cell survival
Article Snippet: Secondary antibodies: HRP-conjugated goat anti-mouse, goat anti-rabbit and donkey anti-goat IgG (H+L; 1:10000 Jackson Laboratories).

Immunofluorescence:

Article Title: Complementary Actions of BDNF and Neurotrophin-3 on the Firing Patterns and Synaptic Composition of Motoneurons
Article Snippet: .. Immunofluorescence was visualized with donkey anti-rabbit IgG coupled to fluorescein isothiocyanate (FITC) or Cy2, donkey anti-goat IgG coupled to FITC, tetramethylrhodamine isothiocyanate or Cy3 and donkey mouse IgG coupled Cy5 (The Jackson Laboratory). ..

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  • 85
    The Jackson Laboratory cy2 labeled donkey anti goat igg
    SEB binds to CD28. (A) Phage display peptides selected by affinity for the SEB binding site in CD28 are SEB antagonists that protect mice from killing by SEB. PBMC were induced with SEB alone or with 0.1 µg/ml p c3 , p e12 , or p c9 , a negative control. IL2 and IFN-γ mRNA are shown; β-actin mRNA indicates equal loading of RNA. For p e12 , IL10 was determined (data are shown as means ± SEM ( n = 3 experiments)). Mice ( n = 10 per group) were challenged with 6 µg SEB alone or with 0.2 µg p e12 or 0.5 µg p c3 ; p for survival, 10 −4 . (B–D) Binding of SEB to cell surface CD28. Representative fields of confocal microscopy are shown. In (B), HEK293-T cells were transfected to express CD28-GFP fusion protein (green) and after 48 h incubated for 1 h with Alexa-Fluor-633-labeled SEB (red). In (C), BHK-21 hamster cells were transfected with CD28 cDNA vector and after 48 h incubated successively for 30 min with labeled SEB (red), goat polyclonal αCD28, and <t>Cy2-labeled</t> donkey anti-goat <t>IgG</t> (green). In (D), BHK-21 cells were transfected to express CD28 and after 48 h incubated first with goat polyclonal αCD28 and Cy2-labeled donkey anti-goat IgG (top) and only then with labeled SEB (bottom). (E) Binding of SEB to CD28. SEB, lysozyme, and polyclonal αCD28 (Ab) were separated on duplicate SDS-PAGE gels. Coomassie blue staining (top); far-western blot with CD28-Fc (bottom); M, size marker.
    Cy2 Labeled Donkey Anti Goat Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy2 labeled donkey anti goat igg/product/The Jackson Laboratory
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy2 labeled donkey anti goat igg - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    88
    The Jackson Laboratory alexa 594 conjugated donkey anti goat igg
    Immunostaining of live SK-N-SH cells with ScFvs. Substrate-attached SK-N-SH cells were incubated with scFvs I4 ( A ), I6 ( B ), I13 ( C ), or I27 ( D ), or without scFv ( E ). Bound scFvs were visualized by rabbit antibody against the His-tag followed by incubation with Alexa 488 nm (green)-conjugated goat anti-rabbit <t>IgG</t> and double labeling with goat anti-human extracellular domain of L1 as positive control, followed by <t>Alexa</t> 594 nm (red)-conjugated donkey anti-goat IgG. Bar in ( E ) indicates 5 µm for all panels. ( F ) Binding of purified scFvs I4, I6, I13, I27 to an SK-N-SH cell lysate was tested by subjecting 50 µg protein to SDS-PAGE in 8% gels under reducing conditions. Western blot analysis was carried out with scFvs I4, I6, I13, I27, and goat anti-human L1 as primary antibodies. Primary antibodies were detected with secondary antibody against His or rabbit anti-goat, respectively. Molecular weight markers are indicated at the left margins in kilodaltons (kDa).
    Alexa 594 Conjugated Donkey Anti Goat Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 594 conjugated donkey anti goat igg/product/The Jackson Laboratory
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    alexa 594 conjugated donkey anti goat igg - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    The Jackson Laboratory donkey anti goat igg hrp conjugated
    Western blot analysis of cytoplasmic(C) and nuclear(N) expression of PIM-2 in various cell lines. Nuclear or cytoplasmic proteins (50 µg) were separated on a 15% SDS-PAGE. Blots were reacted with anti PIM-2 antibodies as primary antibody and <t>HRP</t> conjugated anti rabbit <t>IgG</t> secondary antibody. The membranes were stripped twice and reacted once with anti RCC1 antibody as control for nuclear proteins and once with anti β-tubulin as control for cytoplasmic proteins.
    Donkey Anti Goat Igg Hrp Conjugated, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey anti goat igg hrp conjugated/product/The Jackson Laboratory
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    donkey anti goat igg hrp conjugated - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    The Jackson Laboratory donkey anti goat igg
    Western blot analysis of cytoplasmic(C) and nuclear(N) expression of PIM-2 in various cell lines. Nuclear or cytoplasmic proteins (50 µg) were separated on a 15% SDS-PAGE. Blots were reacted with anti PIM-2 antibodies as primary antibody and <t>HRP</t> conjugated anti rabbit <t>IgG</t> secondary antibody. The membranes were stripped twice and reacted once with anti RCC1 antibody as control for nuclear proteins and once with anti β-tubulin as control for cytoplasmic proteins.
    Donkey Anti Goat Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey anti goat igg/product/The Jackson Laboratory
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    donkey anti goat igg - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    SEB binds to CD28. (A) Phage display peptides selected by affinity for the SEB binding site in CD28 are SEB antagonists that protect mice from killing by SEB. PBMC were induced with SEB alone or with 0.1 µg/ml p c3 , p e12 , or p c9 , a negative control. IL2 and IFN-γ mRNA are shown; β-actin mRNA indicates equal loading of RNA. For p e12 , IL10 was determined (data are shown as means ± SEM ( n = 3 experiments)). Mice ( n = 10 per group) were challenged with 6 µg SEB alone or with 0.2 µg p e12 or 0.5 µg p c3 ; p for survival, 10 −4 . (B–D) Binding of SEB to cell surface CD28. Representative fields of confocal microscopy are shown. In (B), HEK293-T cells were transfected to express CD28-GFP fusion protein (green) and after 48 h incubated for 1 h with Alexa-Fluor-633-labeled SEB (red). In (C), BHK-21 hamster cells were transfected with CD28 cDNA vector and after 48 h incubated successively for 30 min with labeled SEB (red), goat polyclonal αCD28, and Cy2-labeled donkey anti-goat IgG (green). In (D), BHK-21 cells were transfected to express CD28 and after 48 h incubated first with goat polyclonal αCD28 and Cy2-labeled donkey anti-goat IgG (top) and only then with labeled SEB (bottom). (E) Binding of SEB to CD28. SEB, lysozyme, and polyclonal αCD28 (Ab) were separated on duplicate SDS-PAGE gels. Coomassie blue staining (top); far-western blot with CD28-Fc (bottom); M, size marker.

    Journal: PLoS Biology

    Article Title: Binding of Superantigen Toxins into the CD28 Homodimer Interface Is Essential for Induction of Cytokine Genes That Mediate Lethal ShockClarifying the Mechanism of Superantigen Toxicity

    doi: 10.1371/journal.pbio.1001149

    Figure Lengend Snippet: SEB binds to CD28. (A) Phage display peptides selected by affinity for the SEB binding site in CD28 are SEB antagonists that protect mice from killing by SEB. PBMC were induced with SEB alone or with 0.1 µg/ml p c3 , p e12 , or p c9 , a negative control. IL2 and IFN-γ mRNA are shown; β-actin mRNA indicates equal loading of RNA. For p e12 , IL10 was determined (data are shown as means ± SEM ( n = 3 experiments)). Mice ( n = 10 per group) were challenged with 6 µg SEB alone or with 0.2 µg p e12 or 0.5 µg p c3 ; p for survival, 10 −4 . (B–D) Binding of SEB to cell surface CD28. Representative fields of confocal microscopy are shown. In (B), HEK293-T cells were transfected to express CD28-GFP fusion protein (green) and after 48 h incubated for 1 h with Alexa-Fluor-633-labeled SEB (red). In (C), BHK-21 hamster cells were transfected with CD28 cDNA vector and after 48 h incubated successively for 30 min with labeled SEB (red), goat polyclonal αCD28, and Cy2-labeled donkey anti-goat IgG (green). In (D), BHK-21 cells were transfected to express CD28 and after 48 h incubated first with goat polyclonal αCD28 and Cy2-labeled donkey anti-goat IgG (top) and only then with labeled SEB (bottom). (E) Binding of SEB to CD28. SEB, lysozyme, and polyclonal αCD28 (Ab) were separated on duplicate SDS-PAGE gels. Coomassie blue staining (top); far-western blot with CD28-Fc (bottom); M, size marker.

    Article Snippet: CD28 -transfected cells were incubated successively for 30 min each with wt SEB labeled with Alexa Fluor 633 (Molecular Probes), goat polyclonal αCD28 Ab (AF-342-PB, R & D Systems), and Cy2-labeled donkey anti-goat IgG (Jackson Laboratories), washing after each step.

    Techniques: Binding Assay, Mouse Assay, Negative Control, Confocal Microscopy, Transfection, Incubation, Labeling, Plasmid Preparation, SDS Page, Staining, Far Western Blot, Marker

    Immunostaining of live SK-N-SH cells with ScFvs. Substrate-attached SK-N-SH cells were incubated with scFvs I4 ( A ), I6 ( B ), I13 ( C ), or I27 ( D ), or without scFv ( E ). Bound scFvs were visualized by rabbit antibody against the His-tag followed by incubation with Alexa 488 nm (green)-conjugated goat anti-rabbit IgG and double labeling with goat anti-human extracellular domain of L1 as positive control, followed by Alexa 594 nm (red)-conjugated donkey anti-goat IgG. Bar in ( E ) indicates 5 µm for all panels. ( F ) Binding of purified scFvs I4, I6, I13, I27 to an SK-N-SH cell lysate was tested by subjecting 50 µg protein to SDS-PAGE in 8% gels under reducing conditions. Western blot analysis was carried out with scFvs I4, I6, I13, I27, and goat anti-human L1 as primary antibodies. Primary antibodies were detected with secondary antibody against His or rabbit anti-goat, respectively. Molecular weight markers are indicated at the left margins in kilodaltons (kDa).

    Journal: PLoS ONE

    Article Title: Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function

    doi: 10.1371/journal.pone.0052404

    Figure Lengend Snippet: Immunostaining of live SK-N-SH cells with ScFvs. Substrate-attached SK-N-SH cells were incubated with scFvs I4 ( A ), I6 ( B ), I13 ( C ), or I27 ( D ), or without scFv ( E ). Bound scFvs were visualized by rabbit antibody against the His-tag followed by incubation with Alexa 488 nm (green)-conjugated goat anti-rabbit IgG and double labeling with goat anti-human extracellular domain of L1 as positive control, followed by Alexa 594 nm (red)-conjugated donkey anti-goat IgG. Bar in ( E ) indicates 5 µm for all panels. ( F ) Binding of purified scFvs I4, I6, I13, I27 to an SK-N-SH cell lysate was tested by subjecting 50 µg protein to SDS-PAGE in 8% gels under reducing conditions. Western blot analysis was carried out with scFvs I4, I6, I13, I27, and goat anti-human L1 as primary antibodies. Primary antibodies were detected with secondary antibody against His or rabbit anti-goat, respectively. Molecular weight markers are indicated at the left margins in kilodaltons (kDa).

    Article Snippet: Antibody binding was detected by incubation for 1 hour at room temperature with Alexa 488-conjugated donkey anti-rabbit IgG (Jackson Laboratories, Bar Harbor, ME, USA) or Alexa 594-conjugated donkey anti-goat IgG (Jackson Laboratories).

    Techniques: Immunostaining, Incubation, Labeling, Positive Control, Binding Assay, Purification, SDS Page, Western Blot, Molecular Weight

    Western blot analysis of cytoplasmic(C) and nuclear(N) expression of PIM-2 in various cell lines. Nuclear or cytoplasmic proteins (50 µg) were separated on a 15% SDS-PAGE. Blots were reacted with anti PIM-2 antibodies as primary antibody and HRP conjugated anti rabbit IgG secondary antibody. The membranes were stripped twice and reacted once with anti RCC1 antibody as control for nuclear proteins and once with anti β-tubulin as control for cytoplasmic proteins.

    Journal: PLoS ONE

    Article Title: Activation of Cell Cycle Arrest and Apoptosis by the Proto-Oncogene Pim-2

    doi: 10.1371/journal.pone.0034736

    Figure Lengend Snippet: Western blot analysis of cytoplasmic(C) and nuclear(N) expression of PIM-2 in various cell lines. Nuclear or cytoplasmic proteins (50 µg) were separated on a 15% SDS-PAGE. Blots were reacted with anti PIM-2 antibodies as primary antibody and HRP conjugated anti rabbit IgG secondary antibody. The membranes were stripped twice and reacted once with anti RCC1 antibody as control for nuclear proteins and once with anti β-tubulin as control for cytoplasmic proteins.

    Article Snippet: Secondary antibodies included: Mouse anti-Rabbit IgG HRP-conjugated (Sigma); Goat anti-Mouse IgG HRP-conjugated (Bio-Rad); and Donkey anti-Goat IgG HRP-conjugated (Jackson Laboratories).

    Techniques: Western Blot, Expressing, SDS Page