donkey anti goat igg ap conjugate  (Promega)

 
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  • 90
    Name:
    Donkey Anti Goat IgG AP Conjugate
    Description:
    Secondary antibody that binds goat IgG
    Catalog Number:
    v1151
    Price:
    None
    Category:
    Cell Biology Imaging Immunological Detection ELISAs and Antibodies
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    Structured Review

    Promega donkey anti goat igg ap conjugate
    Secondary antibody that binds goat IgG
    https://www.bioz.com/result/donkey anti goat igg ap conjugate/product/Promega
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    donkey anti goat igg ap conjugate - by Bioz Stars, 2020-07
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Desmin enters the nucleus of cardiac stem cells and modulates Nkx2.5 expression by participating in transcription factor complexes that interact with the nkx2.5 gene
    Article Snippet: .. Western blot analysis of the samples was performed by standard procedures with antibodies against mCherry (St. John's Laboratory, STJ34373), desmin (Abcam, ab8592 and Sigma, D8281), histone H3 (Abcam, ab7228), and gp130 (Santa Cruz, sc656), respectively, and secondary-alkaline phosphatase-conjugated antibodies (Promega S372B, S373B, and V1151). ..

    Blocking Assay:

    Article Title: Eco1 is important for DNA damage repair in S. cerevisiae
    Article Snippet: .. Following transfer to a nitrocellulose membrane and blocking, α-phosphoRad53 antibody (Santa Cruz, SC-6749) was used at a concentration of 1:1,000 followed by α-goat-AP (Promega, V1151) at a 1:5,000 concentration. .. For signal detection, BCIP (5-bromo-4-chloro-3-indolyl-phosphate) was used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity in the presence of alkaline phosphatase buffer [100 mM Tris-HCl [pH 9.0], 150 mM NaCl, 1 mM MgCl2 ].

    Concentration Assay:

    Article Title: Eco1 is important for DNA damage repair in S. cerevisiae
    Article Snippet: .. Following transfer to a nitrocellulose membrane and blocking, α-phosphoRad53 antibody (Santa Cruz, SC-6749) was used at a concentration of 1:1,000 followed by α-goat-AP (Promega, V1151) at a 1:5,000 concentration. .. For signal detection, BCIP (5-bromo-4-chloro-3-indolyl-phosphate) was used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity in the presence of alkaline phosphatase buffer [100 mM Tris-HCl [pH 9.0], 150 mM NaCl, 1 mM MgCl2 ].

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  • 90
    Promega donkey anti goat igg alkaline phosphatase conjugate
    Flow cytometric analysis demonstrating the binding of plasminogen to the surface of neisserial cells. Binding of <t>glu‐plg</t> was detected by goat anti‐plasminogen antibody and donkey anti‐goat <t>IgG‐Alexa</t> Fluor488. Fluorescence is displayed as a histogram: area enclosed by the dashed line indicates bacteria treated with primary antibody only; area shaded in gray indicates bacteria treated with secondary only; area enclosed by the solid line indicates bacteria treated with both antibodies.(A). Glu‐plg binds equally to the surface of wild‐type MC58 and MC58Δ cbbA cells. (B). Glu‐plg binds to the surface of N. gonorrhoeae FA1090, N. lactamica ATCC 23970 and a clinical isolate of N. polysaccharea .
    Donkey Anti Goat Igg Alkaline Phosphatase Conjugate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey anti goat igg alkaline phosphatase conjugate/product/Promega
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    donkey anti goat igg alkaline phosphatase conjugate - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    90
    Promega donkey anti goat igg ap conjugate
    Flow cytometric analysis demonstrating the binding of plasminogen to the surface of neisserial cells. Binding of <t>glu‐plg</t> was detected by goat anti‐plasminogen antibody and donkey anti‐goat <t>IgG‐Alexa</t> Fluor488. Fluorescence is displayed as a histogram: area enclosed by the dashed line indicates bacteria treated with primary antibody only; area shaded in gray indicates bacteria treated with secondary only; area enclosed by the solid line indicates bacteria treated with both antibodies.(A). Glu‐plg binds equally to the surface of wild‐type MC58 and MC58Δ cbbA cells. (B). Glu‐plg binds to the surface of N. gonorrhoeae FA1090, N. lactamica ATCC 23970 and a clinical isolate of N. polysaccharea .
    Donkey Anti Goat Igg Ap Conjugate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey anti goat igg ap conjugate/product/Promega
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    donkey anti goat igg ap conjugate - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Flow cytometric analysis demonstrating the binding of plasminogen to the surface of neisserial cells. Binding of glu‐plg was detected by goat anti‐plasminogen antibody and donkey anti‐goat IgG‐Alexa Fluor488. Fluorescence is displayed as a histogram: area enclosed by the dashed line indicates bacteria treated with primary antibody only; area shaded in gray indicates bacteria treated with secondary only; area enclosed by the solid line indicates bacteria treated with both antibodies.(A). Glu‐plg binds equally to the surface of wild‐type MC58 and MC58Δ cbbA cells. (B). Glu‐plg binds to the surface of N. gonorrhoeae FA1090, N. lactamica ATCC 23970 and a clinical isolate of N. polysaccharea .

    Journal: MicrobiologyOpen

    Article Title: Fructose‐1,6‐bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C‐terminal lysine residue

    doi: 10.1002/mbo3.331

    Figure Lengend Snippet: Flow cytometric analysis demonstrating the binding of plasminogen to the surface of neisserial cells. Binding of glu‐plg was detected by goat anti‐plasminogen antibody and donkey anti‐goat IgG‐Alexa Fluor488. Fluorescence is displayed as a histogram: area enclosed by the dashed line indicates bacteria treated with primary antibody only; area shaded in gray indicates bacteria treated with secondary only; area enclosed by the solid line indicates bacteria treated with both antibodies.(A). Glu‐plg binds equally to the surface of wild‐type MC58 and MC58Δ cbbA cells. (B). Glu‐plg binds to the surface of N. gonorrhoeae FA1090, N. lactamica ATCC 23970 and a clinical isolate of N. polysaccharea .

    Article Snippet: Bound glu‐plg was detected using goat anti‐human plg (1:20,000; Rockland Immunochemicals, Pottstown, Pennsylvannia, USA) and donkey anti‐goat IgG‐alkaline phosphatase conjugate (1:5,000; Promega Southampton, Hampshire, UK).

    Techniques: Flow Cytometry, Binding Assay, Fluorescence