dominant negative pmcherryatg5 k130r  (Addgene inc)

 
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    Addgene inc dominant negative pmcherryatg5 k130r
    Dominant Negative Pmcherryatg5 K130r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dominant negative pmcherryatg5 k130r/product/Addgene inc
    Average 86 stars, based on 1 article reviews
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    86/100 stars

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    dominant negative pmcherryatg5 k130r  (Addgene inc)

     
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    Addgene inc dominant negative pmcherryatg5 k130r
    Dominant Negative Pmcherryatg5 K130r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dominant negative pmcherryatg5 k130r/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    91/100 stars

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    dominant negative pmcherryatg5 k130r  (Addgene inc)

     
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    Addgene inc dominant negative pmcherryatg5 k130r
    Inhibition of autophagy reduces cardioprotection. a Protocol for Langendorff perfusion. After stabilization, protein (TAT-μ-gal or Tat-ATG5 <t>K130R</t> ) was infused for 15 min. Hearts then underwent three cycles of 5 min global no-flow ischemia alternating with 5 min reperfusion ( IPC ) followed by 30 min of ischemia and 2 h reperfusion. b Infarct size measured from TTC-stained heart sections is shown ( P < 0.05, N = 5)
    Dominant Negative Pmcherryatg5 K130r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dominant negative pmcherryatg5 k130r/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dominant negative pmcherryatg5 k130r - by Bioz Stars, 2023-10
    91/100 stars

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    1) Product Images from "Autophagy Induced by Ischemic Preconditioning is Essential for Cardioprotection"

    Article Title: Autophagy Induced by Ischemic Preconditioning is Essential for Cardioprotection

    Journal: Journal of Cardiovascular Translational Research

    doi: 10.1007/s12265-010-9189-3

    Inhibition of autophagy reduces cardioprotection. a Protocol for Langendorff perfusion. After stabilization, protein (TAT-μ-gal or Tat-ATG5 K130R ) was infused for 15 min. Hearts then underwent three cycles of 5 min global no-flow ischemia alternating with 5 min reperfusion ( IPC ) followed by 30 min of ischemia and 2 h reperfusion. b Infarct size measured from TTC-stained heart sections is shown ( P < 0.05, N = 5)
    Figure Legend Snippet: Inhibition of autophagy reduces cardioprotection. a Protocol for Langendorff perfusion. After stabilization, protein (TAT-μ-gal or Tat-ATG5 K130R ) was infused for 15 min. Hearts then underwent three cycles of 5 min global no-flow ischemia alternating with 5 min reperfusion ( IPC ) followed by 30 min of ischemia and 2 h reperfusion. b Infarct size measured from TTC-stained heart sections is shown ( P < 0.05, N = 5)

    Techniques Used: Inhibition, Staining

    Cardioprotective agents involve autophagy in HL-1 cells. a Autophagy was scored in GFP-LC3-transfected HL-1 cells exposed to the indicated agents for 10 min. Where indicated, cells were cotransfected with the autophagy inhibitor ATG5 K130R . b HL-1 cells were transfected with GFP-LC3 and with or without ATG5 K130R then subjected to simulated ischemia in the presence or absence of the indicated drug. At the end of 2-h simulated ischemia, cell culture supernatant was harvested for LDH determination
    Figure Legend Snippet: Cardioprotective agents involve autophagy in HL-1 cells. a Autophagy was scored in GFP-LC3-transfected HL-1 cells exposed to the indicated agents for 10 min. Where indicated, cells were cotransfected with the autophagy inhibitor ATG5 K130R . b HL-1 cells were transfected with GFP-LC3 and with or without ATG5 K130R then subjected to simulated ischemia in the presence or absence of the indicated drug. At the end of 2-h simulated ischemia, cell culture supernatant was harvested for LDH determination

    Techniques Used: Transfection, Cell Culture

    Modulation of autophagy by pharmacologic agents and Tat-ATG5 K130R . a HL-1 cells were transfected with GFP-LC3 and treated with the indicated drugs for 10 min. Representative images of treated cells are shown. b Adult rat cardiomyocytes were infected with adenovirus for GFP-LC3 and treated with or without Tat-ATG5 K130R before adding the indicated drugs for 10 min. Representative images of cells are shown
    Figure Legend Snippet: Modulation of autophagy by pharmacologic agents and Tat-ATG5 K130R . a HL-1 cells were transfected with GFP-LC3 and treated with the indicated drugs for 10 min. Representative images of treated cells are shown. b Adult rat cardiomyocytes were infected with adenovirus for GFP-LC3 and treated with or without Tat-ATG5 K130R before adding the indicated drugs for 10 min. Representative images of cells are shown

    Techniques Used: Transfection, Infection

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    Addgene inc dominant negative pmcherryatg5 k130r
    Dominant Negative Pmcherryatg5 K130r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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