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docking tlr4 md 2  (AUTODOCK GmbH)

 
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    AUTODOCK GmbH docking tlr4 md 2
    Comparison of protein expression in Vehicle- and 3α,5α-THP-treated P rats. 3α,5α-THP does not alter the expression of key <t> TLR4-associated </t> proteins. To further clarify the effects of 3α,5α-THP on TLR4 signaling, various proteins involved in the TLR4 pathway were tested. Male and female P rats were treated with 3α,5α-THP (15 mg/kg; 30 min) or vehicle (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min), as previously described. There were no significant sex or treatment effects in protein expression in the hippocampi of P rats of the listed proteins.
    Docking Tlr4 Md 2, supplied by AUTODOCK GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/docking tlr4 md 2/product/AUTODOCK GmbH
    Average 86 stars, based on 1 article reviews
    docking tlr4 md 2 - by Bioz Stars, 2025-04
    86/100 stars

    Images

    1) Product Images from "Novel Inhibitory Actions of Neuroactive Steroid [3α,5α]-3-Hydroxypregnan-20-One on Toll-like Receptor 4-Dependent Neuroimmune Signaling"

    Article Title: Novel Inhibitory Actions of Neuroactive Steroid [3α,5α]-3-Hydroxypregnan-20-One on Toll-like Receptor 4-Dependent Neuroimmune Signaling

    Journal: Biomolecules

    doi: 10.3390/biom14111441

    Comparison of protein expression in Vehicle- and 3α,5α-THP-treated P rats. 3α,5α-THP does not alter the expression of key TLR4-associated proteins. To further clarify the effects of 3α,5α-THP on TLR4 signaling, various proteins involved in the TLR4 pathway were tested. Male and female P rats were treated with 3α,5α-THP (15 mg/kg; 30 min) or vehicle (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min), as previously described. There were no significant sex or treatment effects in protein expression in the hippocampi of P rats of the listed proteins.
    Figure Legend Snippet: Comparison of protein expression in Vehicle- and 3α,5α-THP-treated P rats. 3α,5α-THP does not alter the expression of key TLR4-associated proteins. To further clarify the effects of 3α,5α-THP on TLR4 signaling, various proteins involved in the TLR4 pathway were tested. Male and female P rats were treated with 3α,5α-THP (15 mg/kg; 30 min) or vehicle (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min), as previously described. There were no significant sex or treatment effects in protein expression in the hippocampi of P rats of the listed proteins.

    Techniques Used: Comparison, Expressing

    3α,5α-THP has no effect on TLR4 binding to MD-2 in the hippocampus of male and female P rats. ( A , B ) Hippocampus whole lysate was immunoblotted for the presence of MD-2 in vehicle- (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min) and 3α,5α-THP-treated (15 mg/kg; 30 min) P rats and densiometric comparison of the effect of 3α,5α-THP on MD-2 expression. ( C , E ) Immunoprecipitation of TLR4/MD-2 and densiometric comparison of MD2 between vehicle and 3α,5α-THP-treated P rats (male: t -test, p = 0.56, n = 4/group female: ( t -test, p = 0.46, n = 4/group) ( D , F ). Western blot original images are in the . ns: no significant difference.
    Figure Legend Snippet: 3α,5α-THP has no effect on TLR4 binding to MD-2 in the hippocampus of male and female P rats. ( A , B ) Hippocampus whole lysate was immunoblotted for the presence of MD-2 in vehicle- (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min) and 3α,5α-THP-treated (15 mg/kg; 30 min) P rats and densiometric comparison of the effect of 3α,5α-THP on MD-2 expression. ( C , E ) Immunoprecipitation of TLR4/MD-2 and densiometric comparison of MD2 between vehicle and 3α,5α-THP-treated P rats (male: t -test, p = 0.56, n = 4/group female: ( t -test, p = 0.46, n = 4/group) ( D , F ). Western blot original images are in the . ns: no significant difference.

    Techniques Used: Binding Assay, Comparison, Expressing, Immunoprecipitation, Western Blot

    3α,5α-THP docks TLR4-bound MD-2 and inhibits Lipid A binding to MD-2 in SPR studies. ( A ) Molecular docking shows that 3α,5α-THP favors binding to MD-2, with multiple docking poses of 3α,5α-THP found within the MD-2 binding pocket. ( B ) The top pose of 3α,5α-THP in MD-2 shows multiple π-alkyl bonds formed with key MD-2 residues. ( C ) Two-dimensional representation of 3α,5α-THP binding MD-2, depicting the amino acid interactions. ( D ) SPR studies show Lipid A binds immobilized MD-2 with a K D = 4.3 ± 0.5 µM (k a = 1.5 × 10 2 M −1 S −1 , k d = 6.78 × 10 −4 S −1 ). ( E ) SPR competition assay showing that 3α,5α-THP competitively binds MD2, as increasing concentrations of 3α,5α-THP decrease Lipid A binding ( D ).
    Figure Legend Snippet: 3α,5α-THP docks TLR4-bound MD-2 and inhibits Lipid A binding to MD-2 in SPR studies. ( A ) Molecular docking shows that 3α,5α-THP favors binding to MD-2, with multiple docking poses of 3α,5α-THP found within the MD-2 binding pocket. ( B ) The top pose of 3α,5α-THP in MD-2 shows multiple π-alkyl bonds formed with key MD-2 residues. ( C ) Two-dimensional representation of 3α,5α-THP binding MD-2, depicting the amino acid interactions. ( D ) SPR studies show Lipid A binds immobilized MD-2 with a K D = 4.3 ± 0.5 µM (k a = 1.5 × 10 2 M −1 S −1 , k d = 6.78 × 10 −4 S −1 ). ( E ) SPR competition assay showing that 3α,5α-THP competitively binds MD2, as increasing concentrations of 3α,5α-THP decrease Lipid A binding ( D ).

    Techniques Used: Binding Assay, Competitive Binding Assay



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    docking tlr4 md 2  (AUTODOCK GmbH)
    86
    AUTODOCK GmbH docking tlr4 md 2
    Comparison of protein expression in Vehicle- and 3α,5α-THP-treated P rats. 3α,5α-THP does not alter the expression of key <t> TLR4-associated </t> proteins. To further clarify the effects of 3α,5α-THP on TLR4 signaling, various proteins involved in the TLR4 pathway were tested. Male and female P rats were treated with 3α,5α-THP (15 mg/kg; 30 min) or vehicle (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min), as previously described. There were no significant sex or treatment effects in protein expression in the hippocampi of P rats of the listed proteins.
    Docking Tlr4 Md 2, supplied by AUTODOCK GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/docking tlr4 md 2/product/AUTODOCK GmbH
    Average 86 stars, based on 1 article reviews
    docking tlr4 md 2 - by Bioz Stars, 2025-04
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of protein expression in Vehicle- and 3α,5α-THP-treated P rats. 3α,5α-THP does not alter the expression of key TLR4-associated proteins. To further clarify the effects of 3α,5α-THP on TLR4 signaling, various proteins involved in the TLR4 pathway were tested. Male and female P rats were treated with 3α,5α-THP (15 mg/kg; 30 min) or vehicle (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min), as previously described. There were no significant sex or treatment effects in protein expression in the hippocampi of P rats of the listed proteins.

    Journal: Biomolecules

    Article Title: Novel Inhibitory Actions of Neuroactive Steroid [3α,5α]-3-Hydroxypregnan-20-One on Toll-like Receptor 4-Dependent Neuroimmune Signaling

    doi: 10.3390/biom14111441

    Figure Lengend Snippet: Comparison of protein expression in Vehicle- and 3α,5α-THP-treated P rats. 3α,5α-THP does not alter the expression of key TLR4-associated proteins. To further clarify the effects of 3α,5α-THP on TLR4 signaling, various proteins involved in the TLR4 pathway were tested. Male and female P rats were treated with 3α,5α-THP (15 mg/kg; 30 min) or vehicle (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min), as previously described. There were no significant sex or treatment effects in protein expression in the hippocampi of P rats of the listed proteins.

    Article Snippet: AutoDock Vina (Version 1.2.0) was then used for the docking TLR4:MD-2 and the calculation of docking scores [ ].

    Techniques: Comparison, Expressing

    3α,5α-THP has no effect on TLR4 binding to MD-2 in the hippocampus of male and female P rats. ( A , B ) Hippocampus whole lysate was immunoblotted for the presence of MD-2 in vehicle- (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min) and 3α,5α-THP-treated (15 mg/kg; 30 min) P rats and densiometric comparison of the effect of 3α,5α-THP on MD-2 expression. ( C , E ) Immunoprecipitation of TLR4/MD-2 and densiometric comparison of MD2 between vehicle and 3α,5α-THP-treated P rats (male: t -test, p = 0.56, n = 4/group female: ( t -test, p = 0.46, n = 4/group) ( D , F ). Western blot original images are in the . ns: no significant difference.

    Journal: Biomolecules

    Article Title: Novel Inhibitory Actions of Neuroactive Steroid [3α,5α]-3-Hydroxypregnan-20-One on Toll-like Receptor 4-Dependent Neuroimmune Signaling

    doi: 10.3390/biom14111441

    Figure Lengend Snippet: 3α,5α-THP has no effect on TLR4 binding to MD-2 in the hippocampus of male and female P rats. ( A , B ) Hippocampus whole lysate was immunoblotted for the presence of MD-2 in vehicle- (45% w / v 2-hydroxypropyl-β-cyclodextrin; 30 min) and 3α,5α-THP-treated (15 mg/kg; 30 min) P rats and densiometric comparison of the effect of 3α,5α-THP on MD-2 expression. ( C , E ) Immunoprecipitation of TLR4/MD-2 and densiometric comparison of MD2 between vehicle and 3α,5α-THP-treated P rats (male: t -test, p = 0.56, n = 4/group female: ( t -test, p = 0.46, n = 4/group) ( D , F ). Western blot original images are in the . ns: no significant difference.

    Article Snippet: AutoDock Vina (Version 1.2.0) was then used for the docking TLR4:MD-2 and the calculation of docking scores [ ].

    Techniques: Binding Assay, Comparison, Expressing, Immunoprecipitation, Western Blot

    3α,5α-THP docks TLR4-bound MD-2 and inhibits Lipid A binding to MD-2 in SPR studies. ( A ) Molecular docking shows that 3α,5α-THP favors binding to MD-2, with multiple docking poses of 3α,5α-THP found within the MD-2 binding pocket. ( B ) The top pose of 3α,5α-THP in MD-2 shows multiple π-alkyl bonds formed with key MD-2 residues. ( C ) Two-dimensional representation of 3α,5α-THP binding MD-2, depicting the amino acid interactions. ( D ) SPR studies show Lipid A binds immobilized MD-2 with a K D = 4.3 ± 0.5 µM (k a = 1.5 × 10 2 M −1 S −1 , k d = 6.78 × 10 −4 S −1 ). ( E ) SPR competition assay showing that 3α,5α-THP competitively binds MD2, as increasing concentrations of 3α,5α-THP decrease Lipid A binding ( D ).

    Journal: Biomolecules

    Article Title: Novel Inhibitory Actions of Neuroactive Steroid [3α,5α]-3-Hydroxypregnan-20-One on Toll-like Receptor 4-Dependent Neuroimmune Signaling

    doi: 10.3390/biom14111441

    Figure Lengend Snippet: 3α,5α-THP docks TLR4-bound MD-2 and inhibits Lipid A binding to MD-2 in SPR studies. ( A ) Molecular docking shows that 3α,5α-THP favors binding to MD-2, with multiple docking poses of 3α,5α-THP found within the MD-2 binding pocket. ( B ) The top pose of 3α,5α-THP in MD-2 shows multiple π-alkyl bonds formed with key MD-2 residues. ( C ) Two-dimensional representation of 3α,5α-THP binding MD-2, depicting the amino acid interactions. ( D ) SPR studies show Lipid A binds immobilized MD-2 with a K D = 4.3 ± 0.5 µM (k a = 1.5 × 10 2 M −1 S −1 , k d = 6.78 × 10 −4 S −1 ). ( E ) SPR competition assay showing that 3α,5α-THP competitively binds MD2, as increasing concentrations of 3α,5α-THP decrease Lipid A binding ( D ).

    Article Snippet: AutoDock Vina (Version 1.2.0) was then used for the docking TLR4:MD-2 and the calculation of docking scores [ ].

    Techniques: Binding Assay, Competitive Binding Assay