dntps  (Roche)


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    Structured Review

    Roche dntps
    Dntps, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 863 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/Roche
    Average 94 stars, based on 863 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Mitochondrial Polymorphisms, in The D-Loop Area, Are Associated with Brain Tumors
    Article Snippet: .. The volume of a PCR reaction was 25 μl and contained 50- 100 ng of the DNA, 0.8 μl of the primers, 0.8 μl of MgCl2, 0.5 μl of dNTPs 10 mM, 2.5 μl of PCR buffer (10X), and 0.3 μl of Taq DNA polymerase (Roche Applied Sciences, Germany). .. Moreover, the PCR procedure was carried out according to the following protocol: pre-denaturation phase (5 minutes at 94˚C), followed by 35 cycles of the shorter denaturation phase (50 seconds at 94˚C), annealing step (50 seconds at 55˚C), the extension step (50 seconds at 72˚C), The final stage is the extension phase for 10 minutes at 72˚C.

    Article Title: Characterization of the cyanobacteria and associated bacterial community from an ephemeral wetland in New Zealand.
    Article Snippet: .. New Zealand ephemeral wetlands are ecologically important, containing up to 12% of threatened native plant species and frequently exhibiting conspicuous cyanobacterial growth. .. New Zealand ephemeral wetlands are ecologically important, containing up to 12% of threatened native plant species and frequently exhibiting conspicuous cyanobacterial growth.

    Article Title: Mitochondrial DNA enrichment reduced NUMT contamination in porcine NGS analyses.
    Article Snippet: .. Genetic associations between mitochondrial DNA (mtDNA) and economic traits have been widely reported for pigs, which indicate the importance of mtDNA. .. Genetic associations between mitochondrial DNA (mtDNA) and economic traits have been widely reported for pigs, which indicate the importance of mtDNA.

    Article Title: Identification of a novel non-sense mutation in TBX5 gene in pediatric patients with congenital heart defects
    Article Snippet: .. PCR reaction was carried out in a 25 µl mixture containing 1×PCR buffer, 50 ng genomic DNA as the template, 10 pmol from each forward and reverse primers, 1.7 mmol/L MgCl2 , 0.2 mmol/L in each dNTPs and 3 U Taq DNA polymerase (Roche). .. The amplification program for the cycle reactions were performed in a thermocycler with a three-step PCR protocol: An preliminary denaturation phase at 94°C for 5 minUTES, followed by 35 cycle at 94°C for 35 seconds; 57-63.5°C for 45 seconds (due to different primers); 72°C for 50 seconds and a finishing extension at 72°C for 7 minutes.

    Article Title: Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients.
    Article Snippet: .. The aim of the study was to examine the effects of frequently used polymerase chain reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal growth factor receptor (EGFR) gene promoter region, in order to detect the presence of -216G > T and -191C > A gene variations in non-small-cell lung cancer (NSCLC) patients. ..

    Article Title: Development of Candida auris microsatellite typing and its application on a global collection of isolates
    Article Snippet: .. The PCR reaction for amplification of the STR flanking regions contained 1× Fast Start Taq Polymerase buffer with MgCl2 , 0.2 mM dNTPs, 25 pmol forward (fwd) and (rev) primer, 1 U Faststart Taq polymerase (Roche Diagnostics, Germany) and DNA and was supplemented with water to a final volume of 25 μl. .. A similar setup was used for the multiplex PCR reactions with 4.5 - 20 pmol fwd or rev primers.

    Article Title: Genetic diversity and multiplicity of infection in Fasciola gigantica isolates of Pakistani livestock
    Article Snippet: .. The barcoded PCR was performed with the following conditions: 10 mM dNTPs, 10 μM barcoded forward and reverse primers, 5X KAPA HiFi Fidelity buffer, 0.5 U KAPA HiFi Fidelity Polymerase (KAPA Biosystems, USA), 14μl ddH2O and 2μl of the first-round PCR product. ..

    Amplification:

    Article Title: Development of Candida auris microsatellite typing and its application on a global collection of isolates
    Article Snippet: .. The PCR reaction for amplification of the STR flanking regions contained 1× Fast Start Taq Polymerase buffer with MgCl2 , 0.2 mM dNTPs, 25 pmol forward (fwd) and (rev) primer, 1 U Faststart Taq polymerase (Roche Diagnostics, Germany) and DNA and was supplemented with water to a final volume of 25 μl. .. A similar setup was used for the multiplex PCR reactions with 4.5 - 20 pmol fwd or rev primers.

    Purification:

    Article Title: Characterization of the cyanobacteria and associated bacterial community from an ephemeral wetland in New Zealand.
    Article Snippet: .. New Zealand ephemeral wetlands are ecologically important, containing up to 12% of threatened native plant species and frequently exhibiting conspicuous cyanobacterial growth. .. New Zealand ephemeral wetlands are ecologically important, containing up to 12% of threatened native plant species and frequently exhibiting conspicuous cyanobacterial growth.

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    Roche fitc dntp
    Inhibition of F4/80 + cell apoptosis reduces CD8 + T cell response and increases fungal burden. Wild-type mice were treated daily with Boc-D-FMK apoptosis inhibitor or Z-FA-FMK control peptide or left untreated starting at the day of intratracheal inoculation of 2 × 10 5 live Histoplasma . (A) Lung cells isolated from infected mice at day 4 after infection were stained with TUNEL reagents containing <t>FITC-dNTP</t> and PE-anti-F4/80 antibodies. Apoptotic F4/80 + cells in the lungs were analyzed by flow cytometry. Data shown represent the means ± SD of the total numbers of F4/80 + cells determined for 3 mice used in 3 independent experiments (*, P
    Fitc Dntp, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc dntp/product/Roche
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fitc dntp - by Bioz Stars, 2020-09
    85/100 stars
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    dntps  (Roche)
    94
    Roche dntps
    Primase activity of human PrimPol. ( A ) Human PrimPol has primase activity and can produce de novo primers using <t>rNTPs</t> and <t>dNTPs</t> opposite a poly(dT) template. ( B ) PrimPol ZF-KO lacks de novo primer synthesis activity, suggesting that an intact zinc finger is required for primase activity. ( C ) PrimPol 1–487 also has primase activity similar to the wild-type PrimPol. The unstructured region that is downstream of the zinc finger is therefore not required for primase activity. ( D ) PrimPol 1–354 has no primase activity, which indicates that PrimPol requires a functional zinc finger for primer synthesis.
    Dntps, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 863 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/Roche
    Average 94 stars, based on 863 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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    Inhibition of F4/80 + cell apoptosis reduces CD8 + T cell response and increases fungal burden. Wild-type mice were treated daily with Boc-D-FMK apoptosis inhibitor or Z-FA-FMK control peptide or left untreated starting at the day of intratracheal inoculation of 2 × 10 5 live Histoplasma . (A) Lung cells isolated from infected mice at day 4 after infection were stained with TUNEL reagents containing FITC-dNTP and PE-anti-F4/80 antibodies. Apoptotic F4/80 + cells in the lungs were analyzed by flow cytometry. Data shown represent the means ± SD of the total numbers of F4/80 + cells determined for 3 mice used in 3 independent experiments (*, P

    Journal: Infection and Immunity

    Article Title: Immunization with Apoptotic Phagocytes Containing Histoplasma capsulatum Activates Functional CD8+ T Cells To Protect against Histoplasmosis ▿

    doi: 10.1128/IAI.05350-11

    Figure Lengend Snippet: Inhibition of F4/80 + cell apoptosis reduces CD8 + T cell response and increases fungal burden. Wild-type mice were treated daily with Boc-D-FMK apoptosis inhibitor or Z-FA-FMK control peptide or left untreated starting at the day of intratracheal inoculation of 2 × 10 5 live Histoplasma . (A) Lung cells isolated from infected mice at day 4 after infection were stained with TUNEL reagents containing FITC-dNTP and PE-anti-F4/80 antibodies. Apoptotic F4/80 + cells in the lungs were analyzed by flow cytometry. Data shown represent the means ± SD of the total numbers of F4/80 + cells determined for 3 mice used in 3 independent experiments (*, P

    Article Snippet: For flow cytometric analysis, cells isolated from subcutaneous tissues and lungs were first stained with PE-anti-F4/80 or PE-anti-Gr-1 MAbs at 4°C for 30 min followed by the addition of the TUNEL reaction mixture containing FITC-dNTP and incubated at 37°C in the dark for 1 h. The cells were acquired with a FACSCanto system and analyzed with FACSDiva software.

    Techniques: Inhibition, Mouse Assay, Isolation, Infection, Staining, TUNEL Assay, Flow Cytometry, Cytometry

    iNOS deficiency reduces F4/80 + cell apoptosis and weakens CD8 + T cell response in pulmonary histoplasmosis. Wild-type and iNOS −/− mice were infected intratracheally with 2 × 10 5 live Histoplasma. (A) At day 5 after infection, lung cells were isolated and stained with PE-anti-F4/80 antibody and TUNEL reagents containing FITC-dNTP. F4/80 + TUNEL + apoptotic cells were analyzed by flow cytometry. Data shown represent the means ± SD of the total numbers of apoptotic F4/80 + cells determined for 3 mice used in 3 independent experiments. (B) At day 10 after infection, the mediastinal lymph nodes were harvested and cells were stained with PE-anti-IFN-γ and FITC-anti-CD4 or APC-anti-CD8 antibodies. Data shown represent the means ± SD of the percentages of IFN-γ-producing CD8 + or CD4 + T cells in the total CD8 + or CD4 + T cell population determined for 6 mice used in 3 independent experiments. Cells harvested from uninfected wild-type mice served as controls. The P values were obtained by comparing the results determined for pairs of groups (linked by a bracket) using Student's t test (*, P

    Journal: Infection and Immunity

    Article Title: Immunization with Apoptotic Phagocytes Containing Histoplasma capsulatum Activates Functional CD8+ T Cells To Protect against Histoplasmosis ▿

    doi: 10.1128/IAI.05350-11

    Figure Lengend Snippet: iNOS deficiency reduces F4/80 + cell apoptosis and weakens CD8 + T cell response in pulmonary histoplasmosis. Wild-type and iNOS −/− mice were infected intratracheally with 2 × 10 5 live Histoplasma. (A) At day 5 after infection, lung cells were isolated and stained with PE-anti-F4/80 antibody and TUNEL reagents containing FITC-dNTP. F4/80 + TUNEL + apoptotic cells were analyzed by flow cytometry. Data shown represent the means ± SD of the total numbers of apoptotic F4/80 + cells determined for 3 mice used in 3 independent experiments. (B) At day 10 after infection, the mediastinal lymph nodes were harvested and cells were stained with PE-anti-IFN-γ and FITC-anti-CD4 or APC-anti-CD8 antibodies. Data shown represent the means ± SD of the percentages of IFN-γ-producing CD8 + or CD4 + T cells in the total CD8 + or CD4 + T cell population determined for 6 mice used in 3 independent experiments. Cells harvested from uninfected wild-type mice served as controls. The P values were obtained by comparing the results determined for pairs of groups (linked by a bracket) using Student's t test (*, P

    Article Snippet: For flow cytometric analysis, cells isolated from subcutaneous tissues and lungs were first stained with PE-anti-F4/80 or PE-anti-Gr-1 MAbs at 4°C for 30 min followed by the addition of the TUNEL reaction mixture containing FITC-dNTP and incubated at 37°C in the dark for 1 h. The cells were acquired with a FACSCanto system and analyzed with FACSDiva software.

    Techniques: Mouse Assay, Infection, Isolation, Staining, TUNEL Assay, Flow Cytometry, Cytometry

    Primase activity of human PrimPol. ( A ) Human PrimPol has primase activity and can produce de novo primers using rNTPs and dNTPs opposite a poly(dT) template. ( B ) PrimPol ZF-KO lacks de novo primer synthesis activity, suggesting that an intact zinc finger is required for primase activity. ( C ) PrimPol 1–487 also has primase activity similar to the wild-type PrimPol. The unstructured region that is downstream of the zinc finger is therefore not required for primase activity. ( D ) PrimPol 1–354 has no primase activity, which indicates that PrimPol requires a functional zinc finger for primer synthesis.

    Journal: Nucleic Acids Research

    Article Title: Molecular dissection of the domain architecture and catalytic activities of human PrimPol

    doi: 10.1093/nar/gku214

    Figure Lengend Snippet: Primase activity of human PrimPol. ( A ) Human PrimPol has primase activity and can produce de novo primers using rNTPs and dNTPs opposite a poly(dT) template. ( B ) PrimPol ZF-KO lacks de novo primer synthesis activity, suggesting that an intact zinc finger is required for primase activity. ( C ) PrimPol 1–487 also has primase activity similar to the wild-type PrimPol. The unstructured region that is downstream of the zinc finger is therefore not required for primase activity. ( D ) PrimPol 1–354 has no primase activity, which indicates that PrimPol requires a functional zinc finger for primer synthesis.

    Article Snippet: Typically detection of primase activity was started from incubation of 1μM of the enzyme to be tested in 20 μl reaction volume containing 500 nM homopolymeric ss DNA templates with a biotin modification at the 5′ end (see sequences 1–4 in Supplementary Table S2), 500 μM rNTPs (Invitrogen) or 500 μM dNTPs (Roche), 10 mM Bis-Tris-Propane-HCl (pH 7.0), 10 mM MgCl2 , 50 mM NaCl.

    Techniques: Activity Assay, Functional Assay

    Template-independent extension in the presence of manganese. Human PrimPol was incubated for 30 min with DNA substrate and each of the dNTPs in the presence of manganese. ( A ) Wild-type PrimPol was unable to extend from a ds DNA template with a blunt end, but could extend from a primer annealed to an overhanging template, even synthesising long tracts of homopolymers. ( B ) PrimPol ZF-KO could extend from an overhanging DNA template in the presence of manganese and, consistent with the wild-type, did not extend from a ds DNA substrate. The incorporation of 1 or 2 nucleotides of guanine or adenine opposite an overhanging template suggests that PrimPol ZF-KO incorporates in a low-fidelity template-dependent manner when incubated with overhanging DNA. ( C ) PrimPol 1–487 exhibited a highly similar terminal transferase activity spectrum to the PrimPol ZF-KO . In the presence of manganese, it incorporated bases opposite an overhang in a low fidelity, template-dependent manner. ( D ) PrimPol 1–354 also exhibited low fidelity extension of a primer annealed to an overhanging template in the presence of manganese.

    Journal: Nucleic Acids Research

    Article Title: Molecular dissection of the domain architecture and catalytic activities of human PrimPol

    doi: 10.1093/nar/gku214

    Figure Lengend Snippet: Template-independent extension in the presence of manganese. Human PrimPol was incubated for 30 min with DNA substrate and each of the dNTPs in the presence of manganese. ( A ) Wild-type PrimPol was unable to extend from a ds DNA template with a blunt end, but could extend from a primer annealed to an overhanging template, even synthesising long tracts of homopolymers. ( B ) PrimPol ZF-KO could extend from an overhanging DNA template in the presence of manganese and, consistent with the wild-type, did not extend from a ds DNA substrate. The incorporation of 1 or 2 nucleotides of guanine or adenine opposite an overhanging template suggests that PrimPol ZF-KO incorporates in a low-fidelity template-dependent manner when incubated with overhanging DNA. ( C ) PrimPol 1–487 exhibited a highly similar terminal transferase activity spectrum to the PrimPol ZF-KO . In the presence of manganese, it incorporated bases opposite an overhang in a low fidelity, template-dependent manner. ( D ) PrimPol 1–354 also exhibited low fidelity extension of a primer annealed to an overhanging template in the presence of manganese.

    Article Snippet: Typical reactions were performed in 20 μl volume containing 10 mM Bis-Tris-Propane-HCl (pH 7.0), 10 mM NaCl, 10 mM MgCl2 , 1 mM MnCl2 , 1 mM DTT, 20 nM DNA substrate, 200 μM dNTPs (Roche), with 100 nM recombinant human PrimPol or its variants.

    Techniques: Incubation, Activity Assay

    Polymerase activity and fidelity of human PrimPol. ( A ) Human PrimPol was incubated with dNTPs and substrate at 1, 3, 5 and 30 min time points. PrimPol was proficient at extending an undamaged oligonucleotide template using dNTPs. Human PrimPol did not require an intact zinc finger in order to carry out primer extension, as evidenced by the extension of primers by PrimPol ZF-KO and PrimPol 1–354 . PrimPol 1–487 that lacked the unstructured C-terminus of the protein was also polymerase proficient. PrimPol 1–354 exhibited a higher rate of polymerase activity compared to the other constructs. ( B ) Incorporation of nucleotides opposite two templating cytosine bases. PrimPol was incubated for 5 min with the DNA substrate and each of the dNTPs. All four of these PrimPol constructs inserted two guanine nucleotides opposite two cytosines in Watson–Crick base-pairing manner. PrimPol 1–354 could additionally incorporate a single adenine opposite the first cytosine.

    Journal: Nucleic Acids Research

    Article Title: Molecular dissection of the domain architecture and catalytic activities of human PrimPol

    doi: 10.1093/nar/gku214

    Figure Lengend Snippet: Polymerase activity and fidelity of human PrimPol. ( A ) Human PrimPol was incubated with dNTPs and substrate at 1, 3, 5 and 30 min time points. PrimPol was proficient at extending an undamaged oligonucleotide template using dNTPs. Human PrimPol did not require an intact zinc finger in order to carry out primer extension, as evidenced by the extension of primers by PrimPol ZF-KO and PrimPol 1–354 . PrimPol 1–487 that lacked the unstructured C-terminus of the protein was also polymerase proficient. PrimPol 1–354 exhibited a higher rate of polymerase activity compared to the other constructs. ( B ) Incorporation of nucleotides opposite two templating cytosine bases. PrimPol was incubated for 5 min with the DNA substrate and each of the dNTPs. All four of these PrimPol constructs inserted two guanine nucleotides opposite two cytosines in Watson–Crick base-pairing manner. PrimPol 1–354 could additionally incorporate a single adenine opposite the first cytosine.

    Article Snippet: Typical reactions were performed in 20 μl volume containing 10 mM Bis-Tris-Propane-HCl (pH 7.0), 10 mM NaCl, 10 mM MgCl2 , 1 mM MnCl2 , 1 mM DTT, 20 nM DNA substrate, 200 μM dNTPs (Roche), with 100 nM recombinant human PrimPol or its variants.

    Techniques: Activity Assay, Incubation, Construct

    Processivity of the polymerase activity of PrimPol. PrimPol was pre-incubated for 30 min at 37°C with an undamaged DNA primer-template substrate to allow PrimPol to bind to the DNA. The reaction was initiated by the addition of dNTPs and an excess of sonicated herring sperm DNA (trap) and time points taken at 15, 30, 60, 120 and 360 s. ( A ) After 360 s, wild-type PrimPol incorporated up to 4 nucleotides opposite the template but a significant fraction of enzyme incorporated only 1, 2 or 3 nucleotides (left panel). To confirm that the trap prevents polymerase extending from a second template, the trap was also added into the pre-incubation mix with PrimPol and the DNA substrate. This reaction was supplemented with dNTPs and there is no extension (right panel), thus successfully exhibiting the effectiveness of the trap. ( B ) PrimPol 1–354 also predominantly incorporates up to 4 nucleotides but there were fewer polymerases incorporating only 1, 2 or 3 nucleotides (left panel). The effectiveness of the trap was also successfully confirmed (right panel). ( C ) Percentage of PrimPol molecules incorporating at least n dNTPs for either full-length PrimPol or PrimPol 1–354 , calculated using Equation (1) .

    Journal: Nucleic Acids Research

    Article Title: Molecular dissection of the domain architecture and catalytic activities of human PrimPol

    doi: 10.1093/nar/gku214

    Figure Lengend Snippet: Processivity of the polymerase activity of PrimPol. PrimPol was pre-incubated for 30 min at 37°C with an undamaged DNA primer-template substrate to allow PrimPol to bind to the DNA. The reaction was initiated by the addition of dNTPs and an excess of sonicated herring sperm DNA (trap) and time points taken at 15, 30, 60, 120 and 360 s. ( A ) After 360 s, wild-type PrimPol incorporated up to 4 nucleotides opposite the template but a significant fraction of enzyme incorporated only 1, 2 or 3 nucleotides (left panel). To confirm that the trap prevents polymerase extending from a second template, the trap was also added into the pre-incubation mix with PrimPol and the DNA substrate. This reaction was supplemented with dNTPs and there is no extension (right panel), thus successfully exhibiting the effectiveness of the trap. ( B ) PrimPol 1–354 also predominantly incorporates up to 4 nucleotides but there were fewer polymerases incorporating only 1, 2 or 3 nucleotides (left panel). The effectiveness of the trap was also successfully confirmed (right panel). ( C ) Percentage of PrimPol molecules incorporating at least n dNTPs for either full-length PrimPol or PrimPol 1–354 , calculated using Equation (1) .

    Article Snippet: Typical reactions were performed in 20 μl volume containing 10 mM Bis-Tris-Propane-HCl (pH 7.0), 10 mM NaCl, 10 mM MgCl2 , 1 mM MnCl2 , 1 mM DTT, 20 nM DNA substrate, 200 μM dNTPs (Roche), with 100 nM recombinant human PrimPol or its variants.

    Techniques: Activity Assay, Incubation, Sonication

    PrimPol 1–354 can replicate through CPD and (6–4(PP)) lesions. ( A ) PrimPol 1–354 was incubated with a primer-template substrate in which the template contained a CPD lesion downstream of the primer-template junction in the presence of dNTPs. Time points were taken between 0.5 and 60 min. PrimPol 1–354 extends from the primer up to the CPD, before stalling, it will then added a base opposite the first thymine of the CPD and continue to extend until the end of the template (left panel). PrimPol 1–354 was then incubated with each dNTP to test which nucleotide it incorporates opposite a CPD (right panels). PrimPol 1–354 incorporated adenine opposite the first and second thymine of the CPD. ( B ) The polymerase domain of PrimPol can also perform TLS bypass of a 6–4(PP) lesion immediately downstream of the primer-template junction (left panel). PrimPol 1–354 incorporates either an adenine or cytosine nucleotide opposite the first thymine of the 6–4(PP) (right panels). If an adenine is incorporated opposite the first thymine, a cytosine or thymine is then incorporated opposite the second. If a cytosine is incorporated opposite the first thymine, an adenine, cytosine or thymine will be incorporated opposite the second thymine of the 6–4(PP).

    Journal: Nucleic Acids Research

    Article Title: Molecular dissection of the domain architecture and catalytic activities of human PrimPol

    doi: 10.1093/nar/gku214

    Figure Lengend Snippet: PrimPol 1–354 can replicate through CPD and (6–4(PP)) lesions. ( A ) PrimPol 1–354 was incubated with a primer-template substrate in which the template contained a CPD lesion downstream of the primer-template junction in the presence of dNTPs. Time points were taken between 0.5 and 60 min. PrimPol 1–354 extends from the primer up to the CPD, before stalling, it will then added a base opposite the first thymine of the CPD and continue to extend until the end of the template (left panel). PrimPol 1–354 was then incubated with each dNTP to test which nucleotide it incorporates opposite a CPD (right panels). PrimPol 1–354 incorporated adenine opposite the first and second thymine of the CPD. ( B ) The polymerase domain of PrimPol can also perform TLS bypass of a 6–4(PP) lesion immediately downstream of the primer-template junction (left panel). PrimPol 1–354 incorporates either an adenine or cytosine nucleotide opposite the first thymine of the 6–4(PP) (right panels). If an adenine is incorporated opposite the first thymine, a cytosine or thymine is then incorporated opposite the second. If a cytosine is incorporated opposite the first thymine, an adenine, cytosine or thymine will be incorporated opposite the second thymine of the 6–4(PP).

    Article Snippet: Typical reactions were performed in 20 μl volume containing 10 mM Bis-Tris-Propane-HCl (pH 7.0), 10 mM NaCl, 10 mM MgCl2 , 1 mM MnCl2 , 1 mM DTT, 20 nM DNA substrate, 200 μM dNTPs (Roche), with 100 nM recombinant human PrimPol or its variants.

    Techniques: Incubation