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GenScript dntps
Dntps, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 25 article reviews
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dntps - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Transduction:

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: Paragraph title: Analysis of the SA and JA Signal Transduction Pathways by qRT-PCR ... PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA).

Clone Assay:

Article Title: M2 polarization enhances silica nanoparticle uptake by macrophages
Article Snippet: Standards, from 60 to 0.00006 attomoles of the PCR product cloned into pGEMTeasy (Promega), were run alongside the samples to generate a standard curve. .. The PCR reaction mix consisted of 10x PCR buffer (GenScript), either 2 or 8 mM dNTPs (GenScript), 3–9 mM Mg2+ , 500 nM sense, and antisense primers, either 2.5 or 1.5 pmol of the respective dual-labeled probe, and 2.5 U of Taq DNA Polymerase (GenScript) in a total volume of 25 μl as described in ( , ; ).

Amplification:

Article Title: Population attenuation in zooplankton communities during transoceanic transfer in ballast water
Article Snippet: A 25 μ L PCR cocktail contained 100 ng of genomic DNA, 1 × PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2U of Taq DNA polymerase (Genscript). .. Samples were prepared for amplicon sequencing on an Ion Torrent Personal Genome Machine (PGM) according to the manufacturer's protocols.

Article Title: Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Article Snippet: Paragraph title: DNA extraction, PCR amplification, and pyrosequencing ... Each reaction consisted of approximately 100 ng of genomic DNA, 1× PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2 units of Taq polymerase (Genscript, Piscataway, NJ, USA).

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: .. The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript). .. Total volumes were adjusted to 20 μL on a CFX96 (Bio-Rad) and underwent qPCR with the following cycling conditions: 95 °C for 10 s, 45 cycles (95 °C for 5 s, 60 °C for 30 s).

Article Title: Influence of Artifact Removal on Rare Species Recovery in Natural Complex Communities Using High-Throughput Sequencing
Article Snippet: PCRs were performed in 25 µL cocktail in eight duplicates for each sample to avoid biased amplification. .. Each duplicate contained 100 ng DNA, 1×PCR buffer, 2 mM Mg2+ , 0.2 mM dNTPs, 0.4 µM each primer, and 2 U Taq DNA polymerase (Genscript).

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: .. The mycoplasma genomic DNA was amplified by qPCR using primers and probes and 0.25 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript). .. Total volumes were adjusted to 50 μL on a LightCycler DX480 (Roche) and underwent qPCR with the following cycling conditions: 95 °C for 10 s, 45 cycles (95 °C for 5 s, 60 °C for 1 min).

Article Title: Population Genetic Analysis Infers Migration Pathways of Phytophthora ramorum in US Nurseries
Article Snippet: .. PrMS6, Pr9C3, PrMS39a and b, and PrMS45 were amplified using a PCR program of 1 cycle of 92°C for 2 min, followed by 30 cycles of 92°C for 30 s, 52°C for 30 s, 65°C for 30 s, and 1 cycle of 65°C for 5 min. Fluorescent multiplex PCR reactions were performed in 10-µL volumes with the following final concentrations: 1× GenScript PCR Buffer (10 mM Tris-HCl; 50 mM KCl; 1.5 mM MgCl2 ; 0.1% Triton X-100 buffer), 0.2 µM dNTPs, 3–6 µM of primer pairs, 0.5 U GenScript Taq DNA polymerase (Genscript Corporation, Piscataway, NJ), and 0.5 µL (∼50 ng) DNA template. .. Loci PrMS43a and b were amplified using the following PCR program: 1 cycle of 92°C for 2 min, 35 cycles of 92°C for 30 s, 52°C for 30 s, and 72°C for 1 min, and 1 cycle of 72°C for 45 min.

Article Title: Dispersal influences genetic and acoustic spatial structure for both males and females in a tropical songbird. Dispersal influences genetic and acoustic spatial structure for both males and females in a tropical songbird
Article Snippet: PCR cocktails contained 1.25 μl of 10× PCR buffer (Applied Biosystems), 0.5 μl of MgCl2 (2.5 mmol/L), 0.45 μl of dNTPs (0.2 mmol/L), 0.05 μl of bovine serum albumin, and 0.5 U of Taq (Genscript, Applied Biosystems). .. PCR cocktails for primer sets ThPl 14 , ThPl 20 , and ThPl 30 contained 1 μmol/L each of the forward primer and the IR‐dye‐labeled reverse primer and used the same PCR amplification profiles described in Douglas, Heath, and Mennill ( ).

Polymerase Chain Reaction:

Article Title: Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it
Article Snippet: .. The 50 μl PCR conditions were: 1× Taq polymerase buffer, 200 μM dNTPs, and 2.5 U Taq polymerase (Genscript, E00007), 24 cycles using 1 μM each Primer1F and Primer1R ( ) with denaturation at 94°C for 15 s, annealing at 58°C for 30 s, extension at 70°C for 30 s, and a final extension at 70°C for 5 min. .. The PCR products were resolved on a 2.5% agarose gel, and the bands visualized using ethidium bromide, excised and extracted using QuickClean II Gel Extraction Kit (Genscript, L00418).

Article Title: Population attenuation in zooplankton communities during transoceanic transfer in ballast water
Article Snippet: .. A 25 μ L PCR cocktail contained 100 ng of genomic DNA, 1 × PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2U of Taq DNA polymerase (Genscript). .. PCR cycling parameters consisted of an initial denaturation step at 95°C for 5 min, followed by 25 cycles of 95°C for 30 s, 50°C for 30 s, 72°C for 90 s, and a final elongation step at 72°C for 10 min. Two PCR replicates were prepared for each sample.

Article Title: Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Article Snippet: .. Each reaction consisted of approximately 100 ng of genomic DNA, 1× PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2 units of Taq polymerase (Genscript, Piscataway, NJ, USA). .. PCR cycling parameters consisted of an initial denaturation step at 95°C for 5 min, followed by 25 amplification cycles of 95°C for 30 sec, 50°C for 30 sec, 72°C for 90 sec, and a final elongation step at 72°C for 10 min. All PCR products were cleaned to remove short products using Solid Phase Reversible Immobilisation (SPRI) paramagnetic bead-based method (ChargeSwitch, Invitrogen, Carlsbad, CA, USA).

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: .. Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA). ..

Article Title: Influence of Artifact Removal on Rare Species Recovery in Natural Complex Communities Using High-Throughput Sequencing
Article Snippet: Paragraph title: DNA Isolation, PCR and Pyrosequencing ... Each duplicate contained 100 ng DNA, 1×PCR buffer, 2 mM Mg2+ , 0.2 mM dNTPs, 0.4 µM each primer, and 2 U Taq DNA polymerase (Genscript).

Article Title: M2 polarization enhances silica nanoparticle uptake by macrophages
Article Snippet: .. The PCR reaction mix consisted of 10x PCR buffer (GenScript), either 2 or 8 mM dNTPs (GenScript), 3–9 mM Mg2+ , 500 nM sense, and antisense primers, either 2.5 or 1.5 pmol of the respective dual-labeled probe, and 2.5 U of Taq DNA Polymerase (GenScript) in a total volume of 25 μl as described in ( , ; ). .. Fluorescence Microscopy Cells were seeded into a SensoPlateTM 24-well glass-bottom plate (Greiner Bio-One) at a density of 1.5 × 105 cells/well in 1 ml Macrophage-SFM supplemented with the respective cytokines and allowed to adhere overnight.

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: .. PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA). .. The PCR cycles were 3 min at 95°C, followed by 40 cycles of 30 s at 95°C, 30 s at 57°C, and 30 s at 72°C, followed by a final extension of 72°C for 7 min. PCR products were visualized by staining with 1:1000 dilution of GelRed® (Invitrogen, Carlsbad, CA, USA) added to the loading dye, after separation on a 1.4% agarose gel.

Article Title: Population Genetic Analysis Infers Migration Pathways of Phytophthora ramorum in US Nurseries
Article Snippet: .. PrMS6, Pr9C3, PrMS39a and b, and PrMS45 were amplified using a PCR program of 1 cycle of 92°C for 2 min, followed by 30 cycles of 92°C for 30 s, 52°C for 30 s, 65°C for 30 s, and 1 cycle of 65°C for 5 min. Fluorescent multiplex PCR reactions were performed in 10-µL volumes with the following final concentrations: 1× GenScript PCR Buffer (10 mM Tris-HCl; 50 mM KCl; 1.5 mM MgCl2 ; 0.1% Triton X-100 buffer), 0.2 µM dNTPs, 3–6 µM of primer pairs, 0.5 U GenScript Taq DNA polymerase (Genscript Corporation, Piscataway, NJ), and 0.5 µL (∼50 ng) DNA template. .. Loci PrMS43a and b were amplified using the following PCR program: 1 cycle of 92°C for 2 min, 35 cycles of 92°C for 30 s, 52°C for 30 s, and 72°C for 1 min, and 1 cycle of 72°C for 45 min.

Article Title: Dispersal influences genetic and acoustic spatial structure for both males and females in a tropical songbird. Dispersal influences genetic and acoustic spatial structure for both males and females in a tropical songbird
Article Snippet: .. PCR cocktails contained 1.25 μl of 10× PCR buffer (Applied Biosystems), 0.5 μl of MgCl2 (2.5 mmol/L), 0.45 μl of dNTPs (0.2 mmol/L), 0.05 μl of bovine serum albumin, and 0.5 U of Taq (Genscript, Applied Biosystems). .. For the primer sets Tru 08 , Tru 11 , Tru 18 , Tru 20 , Tru 24 , Tru 25 , and RWWR 2c , PCR cocktails included 1 μmol/L each of an M13 tailed‐forward primer, reverse primer, and a 5′ IR‐dye‐labeled M13 primer (GTAAAACGACGGCCAGT).

Picogreen Assay:

Article Title: Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Article Snippet: Each reaction consisted of approximately 100 ng of genomic DNA, 1× PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2 units of Taq polymerase (Genscript, Piscataway, NJ, USA). .. The quality and quantity of DNA was assessed using gel electrophoresis and Quant-iT PicoGreen dsDNA Assay kit (Invitrogen).

Construct:

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA). .. A genetic linkage map was constructed using JoinMap 4.0 (van Ooijen ).

Article Title: In vitro systems for coupling RNAP II transcription to splicing and polyadenylation
Article Snippet: Paragraph title: 2.1. Materials for preparation of CMV-DNA constructs ... 10 mM dNTPs (GenScript, Cat#: C01582).

Real-time Polymerase Chain Reaction:

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: .. The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript). .. Total volumes were adjusted to 20 μL on a CFX96 (Bio-Rad) and underwent qPCR with the following cycling conditions: 95 °C for 10 s, 45 cycles (95 °C for 5 s, 60 °C for 30 s).

Article Title: M2 polarization enhances silica nanoparticle uptake by macrophages
Article Snippet: The CFX96 TouchTM Real-Time PCR Detection System (Bio-Rad) was used for real-time RT-PCR. .. The PCR reaction mix consisted of 10x PCR buffer (GenScript), either 2 or 8 mM dNTPs (GenScript), 3–9 mM Mg2+ , 500 nM sense, and antisense primers, either 2.5 or 1.5 pmol of the respective dual-labeled probe, and 2.5 U of Taq DNA Polymerase (GenScript) in a total volume of 25 μl as described in ( , ; ).

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: .. The mycoplasma genomic DNA was amplified by qPCR using primers and probes and 0.25 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript). .. Total volumes were adjusted to 50 μL on a LightCycler DX480 (Roche) and underwent qPCR with the following cycling conditions: 95 °C for 10 s, 45 cycles (95 °C for 5 s, 60 °C for 1 min).

Stripping Membranes:

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: .. The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript). .. Total volumes were adjusted to 20 μL on a CFX96 (Bio-Rad) and underwent qPCR with the following cycling conditions: 95 °C for 10 s, 45 cycles (95 °C for 5 s, 60 °C for 30 s).

Modification:

Article Title: Dispersal influences genetic and acoustic spatial structure for both males and females in a tropical songbird. Dispersal influences genetic and acoustic spatial structure for both males and females in a tropical songbird
Article Snippet: We developed the new microsatellites primer sets using a modified method of the Fischer and Bachman ( ) microsatellite enrichment procedure (Walter, Ovenden, & Heath, ). .. PCR cocktails contained 1.25 μl of 10× PCR buffer (Applied Biosystems), 0.5 μl of MgCl2 (2.5 mmol/L), 0.45 μl of dNTPs (0.2 mmol/L), 0.05 μl of bovine serum albumin, and 0.5 U of Taq (Genscript, Applied Biosystems).

Infection:

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA). .. Samples with genomic DNA contamination were discarded and cDNA synthesis was repeated after total RNA treatment with a doubled concentration of DNAse I. Primer sequences of were used for PR-4 , whereas primer pairs for PR-1 and PR-5 (LcPR-1 and LcPR-5) were designed using the mRNA sequences available in the Expressed Sequence Tag (EST) library of lentil infected with the C. lentis ( ).

Sequencing:

Article Title: Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it
Article Snippet: Paragraph title: Library preparation procedure for coligo 122 transcripts followed by Sanger sequencing ... The 50 μl PCR conditions were: 1× Taq polymerase buffer, 200 μM dNTPs, and 2.5 U Taq polymerase (Genscript, E00007), 24 cycles using 1 μM each Primer1F and Primer1R ( ) with denaturation at 94°C for 15 s, annealing at 58°C for 30 s, extension at 70°C for 30 s, and a final extension at 70°C for 5 min.

Article Title: Population attenuation in zooplankton communities during transoceanic transfer in ballast water
Article Snippet: A 25 μ L PCR cocktail contained 100 ng of genomic DNA, 1 × PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2U of Taq DNA polymerase (Genscript). .. Samples were prepared for amplicon sequencing on an Ion Torrent Personal Genome Machine (PGM) according to the manufacturer's protocols.

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: Linkage analysis and genetic mapping The fluorescently labeled simple sequence repeats (SSR) markers and the universal soybean linkage panel 1.0 (the USLP 1.0) containing 1536 single nucleotide polymorphism (SNP) loci (Hyten et al. ) were utilized to genotype the mapping population using the Illumina GoldenGate assay (Fan et al. ; Hyten et al. ). .. Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA).

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA). .. Samples with genomic DNA contamination were discarded and cDNA synthesis was repeated after total RNA treatment with a doubled concentration of DNAse I. Primer sequences of were used for PR-4 , whereas primer pairs for PR-1 and PR-5 (LcPR-1 and LcPR-5) were designed using the mRNA sequences available in the Expressed Sequence Tag (EST) library of lentil infected with the C. lentis ( ).

DNA Extraction:

Article Title: Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Article Snippet: Paragraph title: DNA extraction, PCR amplification, and pyrosequencing ... Each reaction consisted of approximately 100 ng of genomic DNA, 1× PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2 units of Taq polymerase (Genscript, Piscataway, NJ, USA).

Article Title: Influence of Artifact Removal on Rare Species Recovery in Natural Complex Communities Using High-Throughput Sequencing
Article Snippet: Paragraph title: DNA Isolation, PCR and Pyrosequencing ... Each duplicate contained 100 ng DNA, 1×PCR buffer, 2 mM Mg2+ , 0.2 mM dNTPs, 0.4 µM each primer, and 2 U Taq DNA polymerase (Genscript).

Nucleic Acid Electrophoresis:

Article Title: Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Article Snippet: Each reaction consisted of approximately 100 ng of genomic DNA, 1× PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2 units of Taq polymerase (Genscript, Piscataway, NJ, USA). .. The quality and quantity of DNA was assessed using gel electrophoresis and Quant-iT PicoGreen dsDNA Assay kit (Invitrogen).

Isolation:

Article Title: Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Article Snippet: DNA extraction, PCR amplification, and pyrosequencing Total genomic DNA was isolated independently from the tissue in the four tubes using DNeasy Blood and Tissue Kits (Qiagen, Venlo, Limburg, Netherlands) following the manufacturer's protocol. .. Each reaction consisted of approximately 100 ng of genomic DNA, 1× PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2 units of Taq polymerase (Genscript, Piscataway, NJ, USA).

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: Paragraph title: DNA and RNA isolation, and detection of viruses ... The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript).

Article Title: M2 polarization enhances silica nanoparticle uptake by macrophages
Article Snippet: Paragraph title: RNA Isolation, Reverse transcription, and Real-Time RT-PCR ... The PCR reaction mix consisted of 10x PCR buffer (GenScript), either 2 or 8 mM dNTPs (GenScript), 3–9 mM Mg2+ , 500 nM sense, and antisense primers, either 2.5 or 1.5 pmol of the respective dual-labeled probe, and 2.5 U of Taq DNA Polymerase (GenScript) in a total volume of 25 μl as described in ( , ; ).

Marker:

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: The protocol for SSR marker analysis was described by Vuong et al. ( ). .. Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA).

Size-exclusion Chromatography:

Article Title: Toward accurate molecular identification of species in complex environmental samples: testing the performance of sequence filtering and clustering methods
Article Snippet: Each reaction consisted of approximately 100 ng of genomic DNA, 1× PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2 units of Taq polymerase (Genscript, Piscataway, NJ, USA). .. PCR cycling parameters consisted of an initial denaturation step at 95°C for 5 min, followed by 25 amplification cycles of 95°C for 30 sec, 50°C for 30 sec, 72°C for 90 sec, and a final elongation step at 72°C for 10 min. All PCR products were cleaned to remove short products using Solid Phase Reversible Immobilisation (SPRI) paramagnetic bead-based method (ChargeSwitch, Invitrogen, Carlsbad, CA, USA).

Labeling:

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: .. Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA). ..

Goldengate Assay:

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: Linkage analysis and genetic mapping The fluorescently labeled simple sequence repeats (SSR) markers and the universal soybean linkage panel 1.0 (the USLP 1.0) containing 1536 single nucleotide polymorphism (SNP) loci (Hyten et al. ) were utilized to genotype the mapping population using the Illumina GoldenGate assay (Fan et al. ; Hyten et al. ). .. Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA).

Reverse Transcription Polymerase Chain Reaction:

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript). .. The RNA viruses were amplified by RT-qPCR using RNA virus strip and One step PrimeScript RT-PCR kit (Perfect Real time) (TaKaRa-Bio).

Quantitative RT-PCR:

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript). .. The RNA viruses were amplified by RT-qPCR using RNA virus strip and One step PrimeScript RT-PCR kit (Perfect Real time) (TaKaRa-Bio).

Article Title: M2 polarization enhances silica nanoparticle uptake by macrophages
Article Snippet: Paragraph title: RNA Isolation, Reverse transcription, and Real-Time RT-PCR ... The PCR reaction mix consisted of 10x PCR buffer (GenScript), either 2 or 8 mM dNTPs (GenScript), 3–9 mM Mg2+ , 500 nM sense, and antisense primers, either 2.5 or 1.5 pmol of the respective dual-labeled probe, and 2.5 U of Taq DNA Polymerase (GenScript) in a total volume of 25 μl as described in ( , ; ).

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: Paragraph title: Analysis of the SA and JA Signal Transduction Pathways by qRT-PCR ... PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA).

Gel Extraction:

Article Title: Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it
Article Snippet: The 50 μl PCR conditions were: 1× Taq polymerase buffer, 200 μM dNTPs, and 2.5 U Taq polymerase (Genscript, E00007), 24 cycles using 1 μM each Primer1F and Primer1R ( ) with denaturation at 94°C for 15 s, annealing at 58°C for 30 s, extension at 70°C for 30 s, and a final extension at 70°C for 5 min. .. The PCR products were resolved on a 2.5% agarose gel, and the bands visualized using ethidium bromide, excised and extracted using QuickClean II Gel Extraction Kit (Genscript, L00418).

IA:

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: .. Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA). ..

Purification:

Article Title: Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C
Article Snippet: Each PCR included 40–50 ng of template DNA, 0.13 μM of labeled forward primer (Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ, USA). .. After purified with the Whatman PCR cleanup procedure (Whatman Inc., Piscataway, NJ, USA), PCR products were analyzed with the ABI 3100 or 3730 DNA sequencer (Applied Biosystems, Foster City, CA, USA) and the GeneMapper 3.7 program (Applied Biosystems, Foster City, CA, USA).

Article Title: Influence of Artifact Removal on Rare Species Recovery in Natural Complex Communities Using High-Throughput Sequencing
Article Snippet: Each duplicate contained 100 ng DNA, 1×PCR buffer, 2 mM Mg2+ , 0.2 mM dNTPs, 0.4 µM each primer, and 2 U Taq DNA polymerase (Genscript). .. PCR cycling parameters consisted of an initial denaturation step at 95°C for 5 min, followed by 25 amplification cycles of 95°C for 30 s, 50°C for 30 s, 72°C for 90 s, and a final elongation step at 72°C for 10 min. We pooled and subsequently purified PCR products of duplicates using the Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based method (Agencourt Bioscience Corporation, MA, USA).

Plasmid Preparation:

Article Title: In vitro systems for coupling RNAP II transcription to splicing and polyadenylation
Article Snippet: Plasmid encoding CMV-Ftz DoF construct containing or lacking BGH polyA signal or encoding constructs of interest. .. 10 mM dNTPs (GenScript, Cat#: C01582).

Software:

Article Title: Population attenuation in zooplankton communities during transoceanic transfer in ballast water
Article Snippet: A 25 μ L PCR cocktail contained 100 ng of genomic DNA, 1 × PCR buffer, 2 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.4 μ mol/L of each primer, and 2U of Taq DNA polymerase (Genscript). .. Raw sequences obtained from Ion Torrent PGM were trimmed (e.g., homopolymer ≤8, maximum number of ambiguous nucleotides = 0) using the software Mothur v. 1.31.2 (Schloss et al. ).

Multiplex Assay:

Article Title: High-sensitivity virus and mycoplasma screening test reveals high prevalence of parvovirus B19 infection in human synovial tissues and bone marrow
Article Snippet: We designed a seven-tube multiplex for detection of 13 DNA viruses (human herpes simplex virus (HSV)1, HSV2, human hepatitis B virus (HBV), BK virus (BKV), human polyomavirus (JCV), EBV, varicella zoster virus (VZV), HHV6, HHV7, HHV8, adenovirus (ADV), cytomegalovirus (CMV), and parvovirus B19), and six-tube multiplex for detection of six RNA viruses (human immunodeficiency virus (HIV)1, HIV2, human T-cell leukemia virus (HTLV)1, HTLV2, West Nile virus (WNV), and human hepatitis C virus (HCV)). .. The DNA viruses were amplified by quantitative PCR (qPCR) using DNA virus strip and 0.1 U taq DNA polymerase (Thermo), anti-taq high (TOYOBO), 3 mM MgCl2 , and 1 mM dNTPs (genscript).

Article Title: Population Genetic Analysis Infers Migration Pathways of Phytophthora ramorum in US Nurseries
Article Snippet: .. PrMS6, Pr9C3, PrMS39a and b, and PrMS45 were amplified using a PCR program of 1 cycle of 92°C for 2 min, followed by 30 cycles of 92°C for 30 s, 52°C for 30 s, 65°C for 30 s, and 1 cycle of 65°C for 5 min. Fluorescent multiplex PCR reactions were performed in 10-µL volumes with the following final concentrations: 1× GenScript PCR Buffer (10 mM Tris-HCl; 50 mM KCl; 1.5 mM MgCl2 ; 0.1% Triton X-100 buffer), 0.2 µM dNTPs, 3–6 µM of primer pairs, 0.5 U GenScript Taq DNA polymerase (Genscript Corporation, Piscataway, NJ), and 0.5 µL (∼50 ng) DNA template. .. Loci PrMS43a and b were amplified using the following PCR program: 1 cycle of 92°C for 2 min, 35 cycles of 92°C for 30 s, 52°C for 30 s, and 72°C for 1 min, and 1 cycle of 72°C for 45 min.

Agarose Gel Electrophoresis:

Article Title: Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it
Article Snippet: The 50 μl PCR conditions were: 1× Taq polymerase buffer, 200 μM dNTPs, and 2.5 U Taq polymerase (Genscript, E00007), 24 cycles using 1 μM each Primer1F and Primer1R ( ) with denaturation at 94°C for 15 s, annealing at 58°C for 30 s, extension at 70°C for 30 s, and a final extension at 70°C for 5 min. .. The PCR products were resolved on a 2.5% agarose gel, and the bands visualized using ethidium bromide, excised and extracted using QuickClean II Gel Extraction Kit (Genscript, L00418).

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: The integrity of RNA was determined by denaturing agarose gel electrophoresis ( ). .. PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA).

Concentration Assay:

Article Title: Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it
Article Snippet: The circularization products were PCI extracted, ethanol precipitated and re-linearized using RNase A (1 mg/ml final concentration in 10 μl TE buffer for 1 h at 37°C). .. The 50 μl PCR conditions were: 1× Taq polymerase buffer, 200 μM dNTPs, and 2.5 U Taq polymerase (Genscript, E00007), 24 cycles using 1 μM each Primer1F and Primer1R ( ) with denaturation at 94°C for 15 s, annealing at 58°C for 30 s, extension at 70°C for 30 s, and a final extension at 70°C for 5 min.

Article Title: In vitro systems for coupling RNAP II transcription to splicing and polyadenylation
Article Snippet: Primers for coupled transcription/splicing/polyadenylation: Make 500 μL aliquots of Forward primer (5′ tgg agg tcg ctg agt agt gc 3′) and Reverse primer (5′ cca cac cct aac tga gac 3′) at a final concentration of 1.6 μM. .. 10 mM dNTPs (GenScript, Cat#: C01582).

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA). .. Samples with genomic DNA contamination were discarded and cDNA synthesis was repeated after total RNA treatment with a doubled concentration of DNAse I. Primer sequences of were used for PR-4 , whereas primer pairs for PR-1 and PR-5 (LcPR-1 and LcPR-5) were designed using the mRNA sequences available in the Expressed Sequence Tag (EST) library of lentil infected with the C. lentis ( ).

CTG Assay:

Article Title: In vitro systems for coupling RNAP II transcription to splicing and polyadenylation
Article Snippet: Primers for coupled transcription/splicing/polyadenylation: Make 500 μL aliquots of Forward primer (5′ tgg agg tcg ctg agt agt gc 3′) and Reverse primer (5′ cca cac cct aac tga gac 3′) at a final concentration of 1.6 μM. .. 10 mM dNTPs (GenScript, Cat#: C01582).

Staining:

Article Title: Genotype-Dependent Interaction of Lentil Lines with Ascochyta lentis
Article Snippet: PCR was conducted in a 20 μL reaction mix containing 4 μL of 1:10 diluted cDNA, 1X taq reaction buffer, 0.13 μM of each primer, 0.25 mM dNTPs, 3 mM MgCl2 , and 1 U Taq polymerase (GenScript, Piscataway, NJ, USA). .. The PCR cycles were 3 min at 95°C, followed by 40 cycles of 30 s at 95°C, 30 s at 57°C, and 30 s at 72°C, followed by a final extension of 72°C for 7 min. PCR products were visualized by staining with 1:1000 dilution of GelRed® (Invitrogen, Carlsbad, CA, USA) added to the loading dye, after separation on a 1.4% agarose gel.

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  • 92
    GenScript dntp standards
    Representative chromatogram of <t>dNTP</t> levels at the LLOQ Ion chromatograms showing relative abundance for dGTP, ATP, <t>dATP,</t> labeled C-13 dATP, dTTP, and dCTP upon injection of 62.5 fmol dNTPs, 1,250 fmol ATP, and 2.5 pmol of labeled C-13 dATP.
    Dntp Standards, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp standards/product/GenScript
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dntp standards - by Bioz Stars, 2020-04
    92/100 stars
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    95
    GenScript dntp mix
    Representative chromatogram of <t>dNTP</t> levels at the LLOQ Ion chromatograms showing relative abundance for dGTP, ATP, <t>dATP,</t> labeled C-13 dATP, dTTP, and dCTP upon injection of 62.5 fmol dNTPs, 1,250 fmol ATP, and 2.5 pmol of labeled C-13 dATP.
    Dntp Mix, supplied by GenScript, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp mix/product/GenScript
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    93
    GenScript dntps
    Representative chromatogram of <t>dNTP</t> levels at the LLOQ Ion chromatograms showing relative abundance for dGTP, ATP, <t>dATP,</t> labeled C-13 dATP, dTTP, and dCTP upon injection of 62.5 fmol dNTPs, 1,250 fmol ATP, and 2.5 pmol of labeled C-13 dATP.
    Dntps, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/GenScript
    Average 93 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

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    Representative chromatogram of dNTP levels at the LLOQ Ion chromatograms showing relative abundance for dGTP, ATP, dATP, labeled C-13 dATP, dTTP, and dCTP upon injection of 62.5 fmol dNTPs, 1,250 fmol ATP, and 2.5 pmol of labeled C-13 dATP.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Fast and sensitive HPLC-MS/MS method for direct quantification of intracellular deoxyribonucleoside triphosphates from tissue and cells

    doi: 10.1016/j.jchromb.2017.10.008

    Figure Lengend Snippet: Representative chromatogram of dNTP levels at the LLOQ Ion chromatograms showing relative abundance for dGTP, ATP, dATP, labeled C-13 dATP, dTTP, and dCTP upon injection of 62.5 fmol dNTPs, 1,250 fmol ATP, and 2.5 pmol of labeled C-13 dATP.

    Article Snippet: The dNTP standards, 2′-deoxyadenosine 5′-triphosphate (dATP), 2′-deoxyguanosine 5′-triphosphate (dGTP), 2′-deoxycytidine 5′-triphosphate (dCTP), and 2′-deoxythymidine 5′-triphosphate (dTTP), were purchased as a mixture from GenScript (Piscataway, NJ, USA).

    Techniques: Labeling, Injection