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Bio-Rad dntps
Incorporation of <t>Fc1-dUTP</t> into DNA by Klenow fragment and T4 DNA polymerase. The P18.1/T40.1 primer–template pair of Figure 3 was incubated with DNA polymerase and different sets of <t>dNTPs</t> as indicated. DNA fragment lengths in nucleotides are shown on the left.
Dntps, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA"

Article Title: Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA

Journal: Nucleic Acids Research

doi:

Incorporation of Fc1-dUTP into DNA by Klenow fragment and T4 DNA polymerase. The P18.1/T40.1 primer–template pair of Figure 3 was incubated with DNA polymerase and different sets of dNTPs as indicated. DNA fragment lengths in nucleotides are shown on the left.
Figure Legend Snippet: Incorporation of Fc1-dUTP into DNA by Klenow fragment and T4 DNA polymerase. The P18.1/T40.1 primer–template pair of Figure 3 was incubated with DNA polymerase and different sets of dNTPs as indicated. DNA fragment lengths in nucleotides are shown on the left.

Techniques Used: Incubation

Related Articles

High Performance Liquid Chromatography:

Article Title: Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA
Article Snippet: .. To generate the duplex for HPLC–ECD, the P18.1 and T40.1 oligonucleotides (4 µM each) were annealed before incubation in 240 µl of reaction mixture containing 6.7 mM Tris–HCl pH 8.8, 6.6 mM MgCl2 , 1 mM DTT, 16.8 mM (NH4 )2 SO4 , 200 µM dNTPs (except dTTP), 200 µM Fc1-dUTP and 0.25 U/µl Klenow fragment at room temperature for 20 min. Low molecular weight components were removed with a Bio-Spin 30 chromatography column (Bio-Rad). .. The eluate was successively extracted with equal volumes of phenol/chloroform (1:1) and chloroform.

SYBR Green Assay:

Article Title: Brain-Derived Neurotrophic Factor in Patients with Huntington's Disease
Article Snippet: .. All reactions were performed in a total volume of 25 µl that contained 5 µl of cDNA (1∶10 dilution of reverse transcribed samples for BDNF, ROCK1, ANXA1, GAPDH, B2M and ß-actin amplification or 1∶200 dilution of reverse transcribed samples for EAR amplification), 50 mM KCl, 20 mM Tris-HCI, pH 8.4, 0.2 mM dNTPs, 25 units/ml iTaq DNA polymerase, 3 mM MgCl2 , SYBR Green I with 10 nM fluorescein and stabilisers (iQTM SYBR Green Supermix-Biorad, Hercules, CA, USA), and 0.3 µM each of forward and reverse primers. .. Fluorescence was quantified during the 60°C annealing step, and product formation was confirmed with a melting curve analysis (55°C–94°C).

Article Title: Telomere Length and Mental Well-Being in Elderly Men from the Netherlands and Greece
Article Snippet: .. The quantitative polymerase chain reaction (qPCR) was performed using a BioRad MyiQ iCycler Single Color RT-PCR detection system using iQ™ SYBR® Green Supermix, containing iTaq Polymerase, dNTPs, SYBRGreen I, and buffers (BioRad, CA, USA). ..

Multiplex Assay:

Article Title: Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor
Article Snippet: .. Both probes and primer sets were used in each reaction with the following optimized multiplex PCR protocol: 12.5 μ L iQ Supermix (BioRad), 2.5 μ L 10 mM dNTPs, 2 μ L 50 mM MgCl2 , 0.5 μ L iTaq (BioRad), and 0.5 μ L cDNA template. ..

Amplification:

Article Title: Brain-Derived Neurotrophic Factor in Patients with Huntington's Disease
Article Snippet: .. All reactions were performed in a total volume of 25 µl that contained 5 µl of cDNA (1∶10 dilution of reverse transcribed samples for BDNF, ROCK1, ANXA1, GAPDH, B2M and ß-actin amplification or 1∶200 dilution of reverse transcribed samples for EAR amplification), 50 mM KCl, 20 mM Tris-HCI, pH 8.4, 0.2 mM dNTPs, 25 units/ml iTaq DNA polymerase, 3 mM MgCl2 , SYBR Green I with 10 nM fluorescein and stabilisers (iQTM SYBR Green Supermix-Biorad, Hercules, CA, USA), and 0.3 µM each of forward and reverse primers. .. Fluorescence was quantified during the 60°C annealing step, and product formation was confirmed with a melting curve analysis (55°C–94°C).

Chromatography:

Article Title: Structural and Functional Elucidation of the Mechanism Promoting Error-prone Synthesis by Human DNA Polymerase ? Opposite the 7,8-Dihydro-8-oxo-2?-deoxyguanosine Adduct *
Article Snippet: .. The reaction was terminated by extraction of the remaining dNTPs using a size-exclusion chromatography column (Bio-Spin 6 chromatography column, Bio-Rad). ..

Article Title: Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA
Article Snippet: .. To generate the duplex for HPLC–ECD, the P18.1 and T40.1 oligonucleotides (4 µM each) were annealed before incubation in 240 µl of reaction mixture containing 6.7 mM Tris–HCl pH 8.8, 6.6 mM MgCl2 , 1 mM DTT, 16.8 mM (NH4 )2 SO4 , 200 µM dNTPs (except dTTP), 200 µM Fc1-dUTP and 0.25 U/µl Klenow fragment at room temperature for 20 min. Low molecular weight components were removed with a Bio-Spin 30 chromatography column (Bio-Rad). .. The eluate was successively extracted with equal volumes of phenol/chloroform (1:1) and chloroform.

Size-exclusion Chromatography:

Article Title: Structural and Functional Elucidation of the Mechanism Promoting Error-prone Synthesis by Human DNA Polymerase ? Opposite the 7,8-Dihydro-8-oxo-2?-deoxyguanosine Adduct *
Article Snippet: .. The reaction was terminated by extraction of the remaining dNTPs using a size-exclusion chromatography column (Bio-Spin 6 chromatography column, Bio-Rad). ..

Real-time Polymerase Chain Reaction:

Article Title: Telomere Length and Mental Well-Being in Elderly Men from the Netherlands and Greece
Article Snippet: .. The quantitative polymerase chain reaction (qPCR) was performed using a BioRad MyiQ iCycler Single Color RT-PCR detection system using iQ™ SYBR® Green Supermix, containing iTaq Polymerase, dNTPs, SYBRGreen I, and buffers (BioRad, CA, USA). ..

Polymerase Chain Reaction:

Article Title: Metagenomic Approach to Characterizing Disease Epidemiology in a Disease-Endemic Environment in Northern Thailand
Article Snippet: .. PCRs were performed in a 20-μl volume containing 5 μl (1–100 ng/μL) of DNA template, 400 nM each primer, 200 μM dNTPs, 1.5 mM MgCl2 , 1× PCR buffer, and 0.4 U of iProof High-Fidelity DNA Polymerase (Bio-Rad, Hercules, CA, United States). .. Amplification was performed using a T100 DNA thermal cycler (Bio-Rad) under the following conditions: initial denaturation at 98°C for 3 min; 40 cycles of 98°C for 10 s, 60°C for 20 s, and 72°C for 30 s; and a final extension at 72°C for 5 min. For DNA from all ectoparasites, a fragment of 16S rDNA (V1–V6) region (1,016 bp) was amplified in triplicate in a first-round PCR using primers; 27F-Y (5′-GAGTTTGATCCTGGCTYAG-3′), 1061R (5′-CRRCACGAGCTGACGAC-3′) , and 2.5 μM MidBlocker oligonucleotide to inhibit 16S Candidatus Midichloria mitochondrii amplification ( ).

Article Title: Evidence that the Human Pathogenic Fungus Cryptococcus neoformans var. grubii May Have Evolved in Africa
Article Snippet: .. Each PCR mixture contained 20 µl of 1X PCR buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 1 µM each primer, 0.065 µL iTaq DNA Polymerase (Bio-Rad, Hercules, CA), and approximately 1 ng genomic DNA. .. PCR products were purified using ExoSap-IT purification method (Affymetrix, Cleveland OH), and sequenced using an ABI 3730xl sequencer with Big Dye terminators (Applied Biosystems).

Article Title: Antifungical Activity of Autochthonous Bacillus subtilis Isolated from Prosopis juliflora against Phytopathogenic Fungi
Article Snippet: .. PCR was carried out in 30 µL reaction volume containing 1 µL 10 mM dNTPs, 3 µL 10× PCR buffer, 2.5 µL 50 mM MgCl2 , 0.3 µL (5 units/µL) of Taq polymerase (Bio-Rad), 1.2 µL (10mM) of each primer, and 2 µL template cDNA. .. Amplification was performed in a DNA thermal cycler (Bio-Rad) and consisted of an initial denaturation at 94℃ for 5 min; followed by 35 cycles; denaturation for 30 sec at 94℃, annealing for 30 sec at 58℃, extension at 72℃ for 1 min with final extension was at 72℃ for 7 min. PCR fragments were analysed by agarose gel electrophoresis and ethidium bromide staining and changes in gene expression levels were assessed by visual inspection of band densities.

Article Title: Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor
Article Snippet: .. Both probes and primer sets were used in each reaction with the following optimized multiplex PCR protocol: 12.5 μ L iQ Supermix (BioRad), 2.5 μ L 10 mM dNTPs, 2 μ L 50 mM MgCl2 , 0.5 μ L iTaq (BioRad), and 0.5 μ L cDNA template. ..

Incubation:

Article Title: Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA
Article Snippet: .. To generate the duplex for HPLC–ECD, the P18.1 and T40.1 oligonucleotides (4 µM each) were annealed before incubation in 240 µl of reaction mixture containing 6.7 mM Tris–HCl pH 8.8, 6.6 mM MgCl2 , 1 mM DTT, 16.8 mM (NH4 )2 SO4 , 200 µM dNTPs (except dTTP), 200 µM Fc1-dUTP and 0.25 U/µl Klenow fragment at room temperature for 20 min. Low molecular weight components were removed with a Bio-Spin 30 chromatography column (Bio-Rad). .. The eluate was successively extracted with equal volumes of phenol/chloroform (1:1) and chloroform.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Telomere Length and Mental Well-Being in Elderly Men from the Netherlands and Greece
Article Snippet: .. The quantitative polymerase chain reaction (qPCR) was performed using a BioRad MyiQ iCycler Single Color RT-PCR detection system using iQ™ SYBR® Green Supermix, containing iTaq Polymerase, dNTPs, SYBRGreen I, and buffers (BioRad, CA, USA). ..

Molecular Weight:

Article Title: Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA
Article Snippet: .. To generate the duplex for HPLC–ECD, the P18.1 and T40.1 oligonucleotides (4 µM each) were annealed before incubation in 240 µl of reaction mixture containing 6.7 mM Tris–HCl pH 8.8, 6.6 mM MgCl2 , 1 mM DTT, 16.8 mM (NH4 )2 SO4 , 200 µM dNTPs (except dTTP), 200 µM Fc1-dUTP and 0.25 U/µl Klenow fragment at room temperature for 20 min. Low molecular weight components were removed with a Bio-Spin 30 chromatography column (Bio-Rad). .. The eluate was successively extracted with equal volumes of phenol/chloroform (1:1) and chloroform.

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    Bio-Rad α thio dntp
    Targeted disruption of the Cx26 gene and transcriptional analysis. ( A ) Mouse Cx26 wild-type gene with noncoding exon 1 ( Ex1 ) and exon 2 ( Ex2 ) harboring the complete reading frame. ( B ) Targeting vector: a 1,065-bp fragment of Ex2 was replaced by the neo cassette driven by the PGK promoter. A herpes simplex virus TK cassette was inserted at the 5′ end. The construct was linearized by digestion with NotI, and sticky ends were filled with <t>α-thio</t> <t>dNTP.</t> ( C ) Mutated, recombinant Cx26 locus and informative restriction fragments, to be compared with the corresponding fragments, in A . The 12- and 7.3-kb fragments represent wild-type and targeted alleles, respectively. The localization of the 3′-external probe and the position of the primers used to genotype embryo yolk sac DNA are shown. PCR primers were selected to generate a 1,118-bp DNA product indicative of the wild-type allele or a 827-bp product due to the targeted Cx26 allele.
    α Thio Dntp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α thio dntp/product/Bio-Rad
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    α thio dntp - by Bioz Stars, 2020-12
    80/100 stars
      Buy from Supplier

    93
    Bio-Rad dntps
    Incorporation of <t>Fc1-dUTP</t> into DNA by Klenow fragment and T4 DNA polymerase. The P18.1/T40.1 primer–template pair of Figure 3 was incubated with DNA polymerase and different sets of <t>dNTPs</t> as indicated. DNA fragment lengths in nucleotides are shown on the left.
    Dntps, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/Bio-Rad
    Average 93 stars, based on 218 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-12
    93/100 stars
      Buy from Supplier

    92
    Bio-Rad m dntp
    Incorporation of <t>Fc1-dUTP</t> into DNA by Klenow fragment and T4 DNA polymerase. The P18.1/T40.1 primer–template pair of Figure 3 was incubated with DNA polymerase and different sets of <t>dNTPs</t> as indicated. DNA fragment lengths in nucleotides are shown on the left.
    M Dntp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dntp/product/Bio-Rad
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dntp - by Bioz Stars, 2020-12
    92/100 stars
      Buy from Supplier

    Image Search Results


    Targeted disruption of the Cx26 gene and transcriptional analysis. ( A ) Mouse Cx26 wild-type gene with noncoding exon 1 ( Ex1 ) and exon 2 ( Ex2 ) harboring the complete reading frame. ( B ) Targeting vector: a 1,065-bp fragment of Ex2 was replaced by the neo cassette driven by the PGK promoter. A herpes simplex virus TK cassette was inserted at the 5′ end. The construct was linearized by digestion with NotI, and sticky ends were filled with α-thio dNTP. ( C ) Mutated, recombinant Cx26 locus and informative restriction fragments, to be compared with the corresponding fragments, in A . The 12- and 7.3-kb fragments represent wild-type and targeted alleles, respectively. The localization of the 3′-external probe and the position of the primers used to genotype embryo yolk sac DNA are shown. PCR primers were selected to generate a 1,118-bp DNA product indicative of the wild-type allele or a 827-bp product due to the targeted Cx26 allele.

    Journal: The Journal of Cell Biology

    Article Title: Transplacental Uptake of Glucose Is Decreased in Embryonic Lethal Connexin26-deficient Mice

    doi:

    Figure Lengend Snippet: Targeted disruption of the Cx26 gene and transcriptional analysis. ( A ) Mouse Cx26 wild-type gene with noncoding exon 1 ( Ex1 ) and exon 2 ( Ex2 ) harboring the complete reading frame. ( B ) Targeting vector: a 1,065-bp fragment of Ex2 was replaced by the neo cassette driven by the PGK promoter. A herpes simplex virus TK cassette was inserted at the 5′ end. The construct was linearized by digestion with NotI, and sticky ends were filled with α-thio dNTP. ( C ) Mutated, recombinant Cx26 locus and informative restriction fragments, to be compared with the corresponding fragments, in A . The 12- and 7.3-kb fragments represent wild-type and targeted alleles, respectively. The localization of the 3′-external probe and the position of the primers used to genotype embryo yolk sac DNA are shown. PCR primers were selected to generate a 1,118-bp DNA product indicative of the wild-type allele or a 827-bp product due to the targeted Cx26 allele.

    Article Snippet: After linearizing by NotI digestion, the targeting vector (25 μg) was treated with Klenow enzyme in the presence of α-thio dNTP and electroporated into trypsinized and suspended ES cells (2 × 107 ) in 0.5 ml medium of a Hepes-based electroporation buffer, using a gene pulser (Bio-Rad, Munich, Germany).

    Techniques: Plasmid Preparation, Construct, Recombinant, Polymerase Chain Reaction

    Incorporation of Fc1-dUTP into DNA by Klenow fragment and T4 DNA polymerase. The P18.1/T40.1 primer–template pair of Figure 3 was incubated with DNA polymerase and different sets of dNTPs as indicated. DNA fragment lengths in nucleotides are shown on the left.

    Journal: Nucleic Acids Research

    Article Title: Ferrocene conjugates of dUTP for enzymatic redox labelling of DNA

    doi:

    Figure Lengend Snippet: Incorporation of Fc1-dUTP into DNA by Klenow fragment and T4 DNA polymerase. The P18.1/T40.1 primer–template pair of Figure 3 was incubated with DNA polymerase and different sets of dNTPs as indicated. DNA fragment lengths in nucleotides are shown on the left.

    Article Snippet: To generate the duplex for HPLC–ECD, the P18.1 and T40.1 oligonucleotides (4 µM each) were annealed before incubation in 240 µl of reaction mixture containing 6.7 mM Tris–HCl pH 8.8, 6.6 mM MgCl2 , 1 mM DTT, 16.8 mM (NH4 )2 SO4 , 200 µM dNTPs (except dTTP), 200 µM Fc1-dUTP and 0.25 U/µl Klenow fragment at room temperature for 20 min. Low molecular weight components were removed with a Bio-Spin 30 chromatography column (Bio-Rad).

    Techniques: Incubation