dntps taq dna polymerase  (TaKaRa)

 
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    Name:
    TaKaRa Taq DNA Polymerase
    Description:
    TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT 1 strain and is suitable for routine PCR applications
    Catalog Number:
    r001c
    Price:
    None
    Size:
    3 000 Units
    Category:
    Takara Taq and premix Takara Taq products Standard PCR PCR
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    Structured Review

    TaKaRa dntps taq dna polymerase
    TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT 1 strain and is suitable for routine PCR applications
    https://www.bioz.com/result/dntps taq dna polymerase/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dntps taq dna polymerase - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of a Gene Essential for Sheathed Structure Formation in Sphaerotilus natans, a Filamentous Sheathed Bacterium
    Article Snippet: .. The thermal cycling protocol consisted of an initial denaturation step at 95°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min. Adenine was added to the 3′ terminus of the PCR product by using Taq polymerase (Takara Shuzo), and the product was cloned into pT7Blue by TA cloning. .. A unique Asc I site within sthA in the PCR product was used as the insertion site of the Kmr gene cassette as follows.

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan). .. The PCR products were analyzed by 1.5% agarose gel electrophoresis, purified using a Gel Extraction Kit (Tiangen Biotech, Beijing, China), cloned into the pMD18-T vector (TaKaRa, Japan) and sequenced commercially (Sangon, China).

    Amplification:

    Article Title: Identification of a Gene Essential for Sheathed Structure Formation in Sphaerotilus natans, a Filamentous Sheathed Bacterium
    Article Snippet: A 2.3-kb DNA fragment situated entirely within the sthA gene was amplified by PCR by using primers 5′-TGACGCAGTTGGTACAAGTC-3′ (upstream region of sthA ) and 5′-AGATCCTTCAGGCGGATGCT-3′ (downstream region of sthA ) and PyroBEST DNA polymerase according to the instructions of the supplier (Takara Shuzo). .. The thermal cycling protocol consisted of an initial denaturation step at 95°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min. Adenine was added to the 3′ terminus of the PCR product by using Taq polymerase (Takara Shuzo), and the product was cloned into pT7Blue by TA cloning.

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer
    Article Snippet: .. The results showed that the use of 0.1–0.15 U of high-fidelity DNA polymerase combined with 1 U of Taq DNA polymerase in per 20 μL reaction resulted in the best discrimination and amplification effects ( ). .. Thus, a mix of 0.1–0.15 U of high-fidelity DNA polymerase with 1 U of Taq DNA polymerase in per 20 μL reaction was selected for the subsequent experiments.

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: The viral cDNA was amplified in six distinct overlapping fragments by PCR with six pairs of primers in individual reactions. .. PCR amplifications were performed in separate reactions containing cDNA, 1 pmol of primers, 0.4 mM deoxynucleoside triphosphates, 10% dimethyl sulfoxide, and proofreading TaKaRa Taq polymerase (Takara Shuzo Co., Ltd.) in a buffer provided by the manufacturer.

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: .. For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. We have used Pfu DNA polymerase in PCR amplification of RV sequence and found that this enzyme produced smaller amounts of DNA than TaKaRa Taq polymerase and also amplified DNA fragments over 3 kb poorly.

    Mass Spectrometry:

    Article Title: Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by Deep Vent (exo− ) DNA polymerase
    Article Snippet: Taq DNA polymerase was purchased from TaKaRa. .. The structure of the 30-mer sequence shown in was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on an ultraflex TOF/TOF (Bruker Daltonics).

    Synthesized:

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: Standard polymerase chain reaction reactions and sequencing Primer pairs were designed using Primer 5.0 and then synthesized commercially (Sangon, China). .. The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan).

    TA Cloning:

    Article Title: Identification of a Gene Essential for Sheathed Structure Formation in Sphaerotilus natans, a Filamentous Sheathed Bacterium
    Article Snippet: .. The thermal cycling protocol consisted of an initial denaturation step at 95°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min. Adenine was added to the 3′ terminus of the PCR product by using Taq polymerase (Takara Shuzo), and the product was cloned into pT7Blue by TA cloning. .. A unique Asc I site within sthA in the PCR product was used as the insertion site of the Kmr gene cassette as follows.

    Construct:

    Article Title: Identification of a Gene Essential for Sheathed Structure Formation in Sphaerotilus natans, a Filamentous Sheathed Bacterium
    Article Snippet: The plasmid used for double-crossover integration was constructed as follows. .. The thermal cycling protocol consisted of an initial denaturation step at 95°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min. Adenine was added to the 3′ terminus of the PCR product by using Taq polymerase (Takara Shuzo), and the product was cloned into pT7Blue by TA cloning.

    Electrophoresis:

    Article Title: Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence
    Article Snippet: PCR-RFLP Both PCRs were performed in 25 μ L volume containing 2 μ L of the DNA sample, 0.2 μ L of Taq polymerase (TaKaRa, Dalian, China), 2.5 μ L of 10×Taq buffer (TaKaRa), 2 μ L of diethylnitrophenyl thiophosphate (dNTP, TaKaRa) mixture, 0.5 μ L of each primer (AF/AR or CAF/CAR, 50 mM), and 17.3 μ L of distilled water. .. PCR products and restriction fragments were analyzed after electrophoresis in 2% and 3% agarose gels with 0.2 μ g/mL of ethidium bromide staining and were visualized on a UV transilluminator.

    Expressing:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: We analyzed nocifensive behavior and pERK expression in Vc and C1–C2 in GluR2 and GluR3 delta 7 KI mice and found that the nocifensive threshold of KI mice were significantly higher than wild-type mice, and pERK expression was significantly decrease in KI mice compared to wild-type mice. .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′).

    Modification:

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer
    Article Snippet: .. Development of a modified PR-PCR method using the dideoxynucleotide-blocked primer and a mixture of Taq DNA polymerase and high-fidelity DNA polymerase The experimental results above indicate that two major factors affect the efficiency of the PR-PCR in mutation detection. .. One is that the modification of the 3'-end of a primer with-NH2 or-Pi is unable to completely block primer extension ( ).

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer
    Article Snippet: .. In conclusion, we developed a novel modified PR-PCR method that uses a ddNTP-blocked primer and an enzyme combination of a routine amount of Taq DNA polymerase and a tiny amount of high-fidelity DNA polymerase. .. This method can be used for easy detection of various mutation types, including point mutations, insertions and deletions, with high selectivity and sensitivity.

    Article Title: Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by Deep Vent (exo− ) DNA polymerase
    Article Snippet: General methods The syntheses of chemically modified dNTPs and templates used in this study were described in our previous paper ( ). .. Taq DNA polymerase was purchased from TaKaRa.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Ligation:

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. The six DNA fragments were assembled into a full-length cDNA by ligation after appropriate restriction sites were used (Fig. ).

    other:

    Article Title: Thermostable DNA Ligase-Mediated PCR Production of Circular Plasmid (PPCP) and Its Application in Directed Evolution via In situ Error-Prone PCR
    Article Snippet: Error-prone PPCP using Taq DNA polymerase in the presence of Mn2+ , Tma DNA ligase and the PPCP primer pair from pHsh-kan was performed over the xynA 1 region of pHsh-xynA 1 template (Fig. ).

    Sequencing:

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: PCR amplifications were performed in separate reactions containing cDNA, 1 pmol of primers, 0.4 mM deoxynucleoside triphosphates, 10% dimethyl sulfoxide, and proofreading TaKaRa Taq polymerase (Takara Shuzo Co., Ltd.) in a buffer provided by the manufacturer. .. The PCR program consisted of 1 min at 98°C followed by 25 cycles of 20 s at 98°C, 1 s at 56°C, and varied extension times (depending on the length of each sequence) at 70°C.

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. We have used Pfu DNA polymerase in PCR amplification of RV sequence and found that this enzyme produced smaller amounts of DNA than TaKaRa Taq polymerase and also amplified DNA fragments over 3 kb poorly.

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: Paragraph title: Standard polymerase chain reaction reactions and sequencing ... The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan).

    Article Title: Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by Deep Vent (exo− ) DNA polymerase
    Article Snippet: Taq DNA polymerase was purchased from TaKaRa. .. The structure of the 30-mer sequence shown in was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on an ultraflex TOF/TOF (Bruker Daltonics).

    Nucleic Acid Electrophoresis:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Mutagenesis:

    Article Title: Identification of a Gene Essential for Sheathed Structure Formation in Sphaerotilus natans, a Filamentous Sheathed Bacterium
    Article Snippet: The thermal cycling protocol consisted of an initial denaturation step at 95°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min. Adenine was added to the 3′ terminus of the PCR product by using Taq polymerase (Takara Shuzo), and the product was cloned into pT7Blue by TA cloning. .. A 1.2-kb Hin cII fragment carrying Kmr prepared from pUC4K was inserted into the Asc I site (blunted by T4 DNA polymerase) of sthA in the same orientation without producing a polar mutation.

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer
    Article Snippet: .. Development of a modified PR-PCR method using the dideoxynucleotide-blocked primer and a mixture of Taq DNA polymerase and high-fidelity DNA polymerase The experimental results above indicate that two major factors affect the efficiency of the PR-PCR in mutation detection. .. One is that the modification of the 3'-end of a primer with-NH2 or-Pi is unable to completely block primer extension ( ).

    Isolation:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Polymerase Chain Reaction:

    Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer
    Article Snippet: .. All reactions were performed in a total volume of 20 μL containing a mixture of 0.5 U of Taq DNA polymerase, 0.3 μM each of forward and reverse primer, 0.2 mM dNTPs, (1×) PCR Buffer and 5 ng (equal to approximately 1×106 copies) of template. .. The PCR cycling condition was pre-denaturation at 98°C for 2 min, followed by 35 cycles of denaturation at 98°C for 10 s, annealing at 55°C for 15 s and extension at 72°C for 15 s. The positive (TB-526PC and TB-531PC) and negative control plasmids were both amplified, regardless of whether WT-F or Mu-F was used.

    Article Title: Identification of a Gene Essential for Sheathed Structure Formation in Sphaerotilus natans, a Filamentous Sheathed Bacterium
    Article Snippet: .. The thermal cycling protocol consisted of an initial denaturation step at 95°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min. Adenine was added to the 3′ terminus of the PCR product by using Taq polymerase (Takara Shuzo), and the product was cloned into pT7Blue by TA cloning. .. A unique Asc I site within sthA in the PCR product was used as the insertion site of the Kmr gene cassette as follows.

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: .. PCR amplifications were performed in separate reactions containing cDNA, 1 pmol of primers, 0.4 mM deoxynucleoside triphosphates, 10% dimethyl sulfoxide, and proofreading TaKaRa Taq polymerase (Takara Shuzo Co., Ltd.) in a buffer provided by the manufacturer. .. The PCR program consisted of 1 min at 98°C followed by 25 cycles of 20 s at 98°C, 1 s at 56°C, and varied extension times (depending on the length of each sequence) at 70°C.

    Article Title: Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence
    Article Snippet: .. PCR-RFLP Both PCRs were performed in 25 μ L volume containing 2 μ L of the DNA sample, 0.2 μ L of Taq polymerase (TaKaRa, Dalian, China), 2.5 μ L of 10×Taq buffer (TaKaRa), 2 μ L of diethylnitrophenyl thiophosphate (dNTP, TaKaRa) mixture, 0.5 μ L of each primer (AF/AR or CAF/CAR, 50 mM), and 17.3 μ L of distilled water. .. PCR cycling parameters were as follows: 1 cycle at 96°C for 5 minutes, then 35 cycles of 96°C for 30 seconds, at 60°C for 30 seconds, and at 72°C for 90 seconds, followed by 1 cycle at 72°C for 7 minutes.

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. We have used Pfu DNA polymerase in PCR amplification of RV sequence and found that this enzyme produced smaller amounts of DNA than TaKaRa Taq polymerase and also amplified DNA fragments over 3 kb poorly.

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: .. The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan). ..

    Mouse Assay:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: Genetic information about GluR2 and GluR3 delta7 KI mice was briefly described previously [ ]. .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: .. For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. We have used Pfu DNA polymerase in PCR amplification of RV sequence and found that this enzyme produced smaller amounts of DNA than TaKaRa Taq polymerase and also amplified DNA fragments over 3 kb poorly.

    Staining:

    Article Title: Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence
    Article Snippet: PCR-RFLP Both PCRs were performed in 25 μ L volume containing 2 μ L of the DNA sample, 0.2 μ L of Taq polymerase (TaKaRa, Dalian, China), 2.5 μ L of 10×Taq buffer (TaKaRa), 2 μ L of diethylnitrophenyl thiophosphate (dNTP, TaKaRa) mixture, 0.5 μ L of each primer (AF/AR or CAF/CAR, 50 mM), and 17.3 μ L of distilled water. .. PCR products and restriction fragments were analyzed after electrophoresis in 2% and 3% agarose gels with 0.2 μ g/mL of ethidium bromide staining and were visualized on a UV transilluminator.

    Activated Clotting Time Assay:

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
    Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

    Purification:

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan). .. The PCR products were analyzed by 1.5% agarose gel electrophoresis, purified using a Gel Extraction Kit (Tiangen Biotech, Beijing, China), cloned into the pMD18-T vector (TaKaRa, Japan) and sequenced commercially (Sangon, China).

    Plasmid Preparation:

    Article Title: Identification of a Gene Essential for Sheathed Structure Formation in Sphaerotilus natans, a Filamentous Sheathed Bacterium
    Article Snippet: The plasmid used for double-crossover integration was constructed as follows. .. The thermal cycling protocol consisted of an initial denaturation step at 95°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min. Adenine was added to the 3′ terminus of the PCR product by using Taq polymerase (Takara Shuzo), and the product was cloned into pT7Blue by TA cloning.

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan). .. The PCR products were analyzed by 1.5% agarose gel electrophoresis, purified using a Gel Extraction Kit (Tiangen Biotech, Beijing, China), cloned into the pMD18-T vector (TaKaRa, Japan) and sequenced commercially (Sangon, China).

    Agarose Gel Electrophoresis:

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan). .. The PCR products were analyzed by 1.5% agarose gel electrophoresis, purified using a Gel Extraction Kit (Tiangen Biotech, Beijing, China), cloned into the pMD18-T vector (TaKaRa, Japan) and sequenced commercially (Sangon, China).

    In Vitro:

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. For synthesis of RNA transcripts in vitro, an SP6 RNA polymerase promoter sequence was positioned immediately upstream of the full-length cDNA by using a primer containing the promoter sequence plus the first 14 nt at the 5′ terminus of RV (M33 strain) ( ).

    Produced:

    Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
    Article Snippet: For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. We have used Pfu DNA polymerase in PCR amplification of RV sequence and found that this enzyme produced smaller amounts of DNA than TaKaRa Taq polymerase and also amplified DNA fragments over 3 kb poorly.

    Gel Extraction:

    Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
    Article Snippet: The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan). .. The PCR products were analyzed by 1.5% agarose gel electrophoresis, purified using a Gel Extraction Kit (Tiangen Biotech, Beijing, China), cloned into the pMD18-T vector (TaKaRa, Japan) and sequenced commercially (Sangon, China).

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    TaKaRa pcr mixture iii
    Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and <t>PCR.</t> ( d ) Amplification efficiency was strongly affected by the presence of SuperScript <t>III.</t> The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.
    Pcr Mixture Iii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mixture iii/product/TaKaRa
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    TaKaRa pcr mixture ii
    Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified <t>cDNA</t> molecules in single cells was measured after the 2nd <t>PCR.</t> ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).
    Pcr Mixture Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mixture ii/product/TaKaRa
    Average 99 stars, based on 1 article reviews
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    TaKaRa sybr premix ex taq ii
    Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified <t>cDNA</t> molecules in single cells was measured after the 2nd <t>PCR.</t> ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).
    Sybr Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr premix ex taq ii/product/TaKaRa
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    TaKaRa premix ex taq
    Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified <t>cDNA</t> molecules in single cells was measured after the 2nd <t>PCR.</t> ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).
    Premix Ex Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Article Snippet: Another 15 cycles of PCR was then performed on the four fractions, each containing 1 μl of the 50 -μl purified PCR products together with 49 μl of PCR mixture III (5-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 1-μM AUP1, 1-μM AUP2 and 2.5-U TaKaRa ExTaqTM HS).

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Purification, Polymerase Chain Reaction, Produced

    Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified cDNA molecules in single cells was measured after the 2nd PCR. ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified cDNA molecules in single cells was measured after the 2nd PCR. ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Single-cell Analysis, Expressing, Amplification, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Reduction of primer-dimer production by introducing VN sequence to UP2 primers. Although the production of primer-dimers by PCR is a big issue in terms of whole-cDNA amplification, it is greatly reduced by the use of UP2 primers with poly(T) plus VN sequences at 3′ termini. Electrophoregrams of PCR products obtained with two different primers (denoted as 1 and 2 in the figures) for the 2nd-strand synthesis are shown in figures ( a1–c2 ). In the figures, ‘a’, ‘b’ and ‘c’ stand for crude 1st PCR products, purified 1st PCR products and 2nd PCR products, respectively. The primers used in the cases of Figures a1, b1 and c1 were UP2 primer having poly(T) sequence at 3′ termini. The primers used in the cases of Figures a2, b2 and c2 were anchored UP2 primers having poly(T) plus VN sequences at the 3′ termini. ( d ) The number of amplified cDNA molecules (EEF1G gene) after the 1st PCR, as well as the 2nd PCR, obtained with the two kinds of primers for 2nd-strand synthesis.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Reduction of primer-dimer production by introducing VN sequence to UP2 primers. Although the production of primer-dimers by PCR is a big issue in terms of whole-cDNA amplification, it is greatly reduced by the use of UP2 primers with poly(T) plus VN sequences at 3′ termini. Electrophoregrams of PCR products obtained with two different primers (denoted as 1 and 2 in the figures) for the 2nd-strand synthesis are shown in figures ( a1–c2 ). In the figures, ‘a’, ‘b’ and ‘c’ stand for crude 1st PCR products, purified 1st PCR products and 2nd PCR products, respectively. The primers used in the cases of Figures a1, b1 and c1 were UP2 primer having poly(T) sequence at 3′ termini. The primers used in the cases of Figures a2, b2 and c2 were anchored UP2 primers having poly(T) plus VN sequences at the 3′ termini. ( d ) The number of amplified cDNA molecules (EEF1G gene) after the 1st PCR, as well as the 2nd PCR, obtained with the two kinds of primers for 2nd-strand synthesis.

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification, Purification

    Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Purification, Polymerase Chain Reaction, Produced

    Change of products produced with 10 7 beads immobilizing RT primers with various densities. Starting material in all cases was 2 pg of mRNA. The error bars are independent amplification replicates. ( a ) Number of amplified cDNA molecules (EEF1G gene) in cDNA libraries after 2nd PCR. ( b ) Number of cDNA molecules (EEF1G gene) produced on beads in RT. ( c ) Electrophoregrams of crude 1st PCR products. ( d ) Electrophoregrams after purifying the crude 1st PCR products showed in the electrophoregrams of graph ‘c’.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Change of products produced with 10 7 beads immobilizing RT primers with various densities. Starting material in all cases was 2 pg of mRNA. The error bars are independent amplification replicates. ( a ) Number of amplified cDNA molecules (EEF1G gene) in cDNA libraries after 2nd PCR. ( b ) Number of cDNA molecules (EEF1G gene) produced on beads in RT. ( c ) Electrophoregrams of crude 1st PCR products. ( d ) Electrophoregrams after purifying the crude 1st PCR products showed in the electrophoregrams of graph ‘c’.

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Produced, Amplification, Polymerase Chain Reaction