dntpack  (Roche)


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    Structured Review

    Roche dntpack
    Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntpack/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dntpack - by Bioz Stars, 2021-07
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
    Article Snippet: Viral loads were determined as previously described [ ], using five μl of the extracted RNA and the RNA UltraSense™ One-Step Quantitative RT-PCR System (Invitrogen, ThermoFisher Scientific), and were expressed as genome copies/ml of serum. .. For genotyping, DNA amplification was performed using the FastStart High Fidelity PCR System, dNTPack (Roche Applied Science, Switzerland), with 10 μl of cDNA and using the primers described in and 2 of the web extra material. .. Consensus sequences were obtained using the ABI Prism® Big Dye™ Terminator Cycle Sequencing Ready Reaction Kit v3·1 (Applied Biosystems, CA, USA) and the ABI Prism 3700 automatic sequencer (Applied Biosystems).

    Article Title: Functional Analysis of the TRIB1 Associated Locus Linked to Plasma Triglycerides and Coronary Artery Disease
    Article Snippet: All PCRs were run on the ABI GenAmp PCR system 9700. .. TRIBAL amplification required 2 rounds of PCR with the Roche FastStart Taq DNA polymerase, dNTPack (Roche Diagnostics). .. For 3′ RACE, in the first round of PCR, each reaction contained a gene‐specific forward primer targeting exon 1 or 2 and 1× GC‐rich solution.

    Article Title: C11orf95-MKL2 is the Resulting Fusion Oncogene of t(11;16)(q13;p13) in Chondroid Lipoma
    Article Snippet: .. Because the BAC FISH studies revealed that the chromosome 16p13 breakpoint was occurring within clone CTD-2118O12 containing a single candidate gene ( MKL2 ), two long-range PCR products, MKL2/913-25107 (24195 bp) and MKL2/175221-194480 (19279 bp), corresponding to a normal control genomic MKL2 gene 5′ and 3′ region respectively, were constructed by amplification with Expand Long Range, dNTPack (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s instructions to further confirm and define the rearrangement of this gene. .. MKL2 long-range PCR primer sets included 5′-TGCCCT AACAGACAACCCTCAATCG-3′/5′-CCCTGGAATGAGGAGGACAGCC-3′ for MKL2/913-25107, and 5′-CCAGGAAGTGAACAGCAGCG-3′/5′-TGATTCCCGA CAGCACCTTG-3′ for MKL2/175221-194480.

    Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
    Article Snippet: A second internal or nested PCR amplifying a shorter fragment was performed to obtain sufficient DNA product for sequencing. .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: Following sample harvesting, purification and dilution 1:100, a two-step second PCR of 17 cycles (5 cycles with annealing temperature of 55°C + 12 cycles with annealing temperature of 70°C) is performed to generate a dual indexed sequencing library (note that the ‘Outer primers’ sequences were as described for the second PCR primer sequences in ( ). .. The first PCR (in the AA chip) is done using the manufacturer recommended enzyme: FastStart High Fidelity PCR System, dNTPack (Roche) while the second PCR is done using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the addition of SYBR green I (LONZA) at a final concentration of X1, to enable real time tracking of amplification. .. Following second PCR, each sample was purified using SPRI beads.

    Article Title: Mutations in TJP2 cause progressive cholestatic liver disease
    Article Snippet: .. PCR amplification was optimized in accordance to the standard PCR protocol using FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science). .. Sequencing reaction was performed using the BigDye® v.1.1 Terminator cycle sequencing kit and the ABI Prism® 3130xl Genetic Analyzer (Life Technologies).

    Polymerase Chain Reaction:

    Article Title: Evidence for positive selection of hepatitis A virus antigenic variants in vaccinated men-having-sex-with men patients: Implications for immunization policies
    Article Snippet: Viral loads were determined as previously described [ ], using five μl of the extracted RNA and the RNA UltraSense™ One-Step Quantitative RT-PCR System (Invitrogen, ThermoFisher Scientific), and were expressed as genome copies/ml of serum. .. For genotyping, DNA amplification was performed using the FastStart High Fidelity PCR System, dNTPack (Roche Applied Science, Switzerland), with 10 μl of cDNA and using the primers described in and 2 of the web extra material. .. Consensus sequences were obtained using the ABI Prism® Big Dye™ Terminator Cycle Sequencing Ready Reaction Kit v3·1 (Applied Biosystems, CA, USA) and the ABI Prism 3700 automatic sequencer (Applied Biosystems).

    Article Title: Functional Analysis of the TRIB1 Associated Locus Linked to Plasma Triglycerides and Coronary Artery Disease
    Article Snippet: All PCRs were run on the ABI GenAmp PCR system 9700. .. TRIBAL amplification required 2 rounds of PCR with the Roche FastStart Taq DNA polymerase, dNTPack (Roche Diagnostics). .. For 3′ RACE, in the first round of PCR, each reaction contained a gene‐specific forward primer targeting exon 1 or 2 and 1× GC‐rich solution.

    Article Title: C11orf95-MKL2 is the Resulting Fusion Oncogene of t(11;16)(q13;p13) in Chondroid Lipoma
    Article Snippet: .. Because the BAC FISH studies revealed that the chromosome 16p13 breakpoint was occurring within clone CTD-2118O12 containing a single candidate gene ( MKL2 ), two long-range PCR products, MKL2/913-25107 (24195 bp) and MKL2/175221-194480 (19279 bp), corresponding to a normal control genomic MKL2 gene 5′ and 3′ region respectively, were constructed by amplification with Expand Long Range, dNTPack (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s instructions to further confirm and define the rearrangement of this gene. .. MKL2 long-range PCR primer sets included 5′-TGCCCT AACAGACAACCCTCAATCG-3′/5′-CCCTGGAATGAGGAGGACAGCC-3′ for MKL2/913-25107, and 5′-CCAGGAAGTGAACAGCAGCG-3′/5′-TGATTCCCGA CAGCACCTTG-3′ for MKL2/175221-194480.

    Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
    Article Snippet: A second internal or nested PCR amplifying a shorter fragment was performed to obtain sufficient DNA product for sequencing. .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

    Article Title: A PEPTIDE ANTAGONIST DISRUPTS NATURAL KILLER CELL INHIBITORY SYNAPSE FORMATION1
    Article Snippet: The KIR2DL3-GFP fusion constructs were generated by subcloning RT-PCR amplified cDNAs encoding KIR2DL3 into plasmid pcDNA3.1 (Invitrogen, Life technologies Ltd, Paisley, UK) already containing the eGFP sequence. .. Substitution of KIR2DL3 residues Tyr282 and Tyr312 with phenylalanine was achieved by sequential site-directed mutagenesis by polymerase chain reaction (PCR) using the Expand High Fidelity PCR System and dNTPack (Roche Diagnostics Ltd, Burgess Hill, UK). .. For expression in NKL, KIR2DL3-GFP was cloned into retroviral vector pIB2 and the Phoenix packaging cell line was used to produce retroviral particles.

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: Following sample harvesting, purification and dilution 1:100, a two-step second PCR of 17 cycles (5 cycles with annealing temperature of 55°C + 12 cycles with annealing temperature of 70°C) is performed to generate a dual indexed sequencing library (note that the ‘Outer primers’ sequences were as described for the second PCR primer sequences in ( ). .. The first PCR (in the AA chip) is done using the manufacturer recommended enzyme: FastStart High Fidelity PCR System, dNTPack (Roche) while the second PCR is done using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the addition of SYBR green I (LONZA) at a final concentration of X1, to enable real time tracking of amplification. .. Following second PCR, each sample was purified using SPRI beads.

    Article Title: Targeting CAG repeat RNAs reduces Huntington’s disease phenotype independently of huntingtin levels
    Article Snippet: Intensity data were extracted using the Agilent Feature Extraction software. ) ( ). .. Equal amounts of RNA samples (1 μg) were treated with DNAse I (Ambion, Thermo Fisher Scientific), and 500 ng was reverse transcribed with the Transcriptor First Strand cDNA Synthesis Kit (Roche) according to the manufacturer’s protocol, and PCR was performed using the FastStart Taq DNA Polymerase, dNTPack (Roche). sCAGs were determined as previously described ( ). ..

    Article Title: Mutations in TJP2 cause progressive cholestatic liver disease
    Article Snippet: .. PCR amplification was optimized in accordance to the standard PCR protocol using FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science). .. Sequencing reaction was performed using the BigDye® v.1.1 Terminator cycle sequencing kit and the ABI Prism® 3130xl Genetic Analyzer (Life Technologies).

    BAC Assay:

    Article Title: C11orf95-MKL2 is the Resulting Fusion Oncogene of t(11;16)(q13;p13) in Chondroid Lipoma
    Article Snippet: .. Because the BAC FISH studies revealed that the chromosome 16p13 breakpoint was occurring within clone CTD-2118O12 containing a single candidate gene ( MKL2 ), two long-range PCR products, MKL2/913-25107 (24195 bp) and MKL2/175221-194480 (19279 bp), corresponding to a normal control genomic MKL2 gene 5′ and 3′ region respectively, were constructed by amplification with Expand Long Range, dNTPack (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s instructions to further confirm and define the rearrangement of this gene. .. MKL2 long-range PCR primer sets included 5′-TGCCCT AACAGACAACCCTCAATCG-3′/5′-CCCTGGAATGAGGAGGACAGCC-3′ for MKL2/913-25107, and 5′-CCAGGAAGTGAACAGCAGCG-3′/5′-TGATTCCCGA CAGCACCTTG-3′ for MKL2/175221-194480.

    Fluorescence In Situ Hybridization:

    Article Title: C11orf95-MKL2 is the Resulting Fusion Oncogene of t(11;16)(q13;p13) in Chondroid Lipoma
    Article Snippet: .. Because the BAC FISH studies revealed that the chromosome 16p13 breakpoint was occurring within clone CTD-2118O12 containing a single candidate gene ( MKL2 ), two long-range PCR products, MKL2/913-25107 (24195 bp) and MKL2/175221-194480 (19279 bp), corresponding to a normal control genomic MKL2 gene 5′ and 3′ region respectively, were constructed by amplification with Expand Long Range, dNTPack (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s instructions to further confirm and define the rearrangement of this gene. .. MKL2 long-range PCR primer sets included 5′-TGCCCT AACAGACAACCCTCAATCG-3′/5′-CCCTGGAATGAGGAGGACAGCC-3′ for MKL2/913-25107, and 5′-CCAGGAAGTGAACAGCAGCG-3′/5′-TGATTCCCGA CAGCACCTTG-3′ for MKL2/175221-194480.

    Construct:

    Article Title: C11orf95-MKL2 is the Resulting Fusion Oncogene of t(11;16)(q13;p13) in Chondroid Lipoma
    Article Snippet: .. Because the BAC FISH studies revealed that the chromosome 16p13 breakpoint was occurring within clone CTD-2118O12 containing a single candidate gene ( MKL2 ), two long-range PCR products, MKL2/913-25107 (24195 bp) and MKL2/175221-194480 (19279 bp), corresponding to a normal control genomic MKL2 gene 5′ and 3′ region respectively, were constructed by amplification with Expand Long Range, dNTPack (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s instructions to further confirm and define the rearrangement of this gene. .. MKL2 long-range PCR primer sets included 5′-TGCCCT AACAGACAACCCTCAATCG-3′/5′-CCCTGGAATGAGGAGGACAGCC-3′ for MKL2/913-25107, and 5′-CCAGGAAGTGAACAGCAGCG-3′/5′-TGATTCCCGA CAGCACCTTG-3′ for MKL2/175221-194480.

    Nested PCR:

    Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
    Article Snippet: A second internal or nested PCR amplifying a shorter fragment was performed to obtain sufficient DNA product for sequencing. .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

    Electrophoresis:

    Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
    Article Snippet: A second internal or nested PCR amplifying a shorter fragment was performed to obtain sufficient DNA product for sequencing. .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

    Agarose Gel Electrophoresis:

    Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
    Article Snippet: A second internal or nested PCR amplifying a shorter fragment was performed to obtain sufficient DNA product for sequencing. .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

    Mutagenesis:

    Article Title: A PEPTIDE ANTAGONIST DISRUPTS NATURAL KILLER CELL INHIBITORY SYNAPSE FORMATION1
    Article Snippet: The KIR2DL3-GFP fusion constructs were generated by subcloning RT-PCR amplified cDNAs encoding KIR2DL3 into plasmid pcDNA3.1 (Invitrogen, Life technologies Ltd, Paisley, UK) already containing the eGFP sequence. .. Substitution of KIR2DL3 residues Tyr282 and Tyr312 with phenylalanine was achieved by sequential site-directed mutagenesis by polymerase chain reaction (PCR) using the Expand High Fidelity PCR System and dNTPack (Roche Diagnostics Ltd, Burgess Hill, UK). .. For expression in NKL, KIR2DL3-GFP was cloned into retroviral vector pIB2 and the Phoenix packaging cell line was used to produce retroviral particles.

    Chromatin Immunoprecipitation:

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: Following sample harvesting, purification and dilution 1:100, a two-step second PCR of 17 cycles (5 cycles with annealing temperature of 55°C + 12 cycles with annealing temperature of 70°C) is performed to generate a dual indexed sequencing library (note that the ‘Outer primers’ sequences were as described for the second PCR primer sequences in ( ). .. The first PCR (in the AA chip) is done using the manufacturer recommended enzyme: FastStart High Fidelity PCR System, dNTPack (Roche) while the second PCR is done using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the addition of SYBR green I (LONZA) at a final concentration of X1, to enable real time tracking of amplification. .. Following second PCR, each sample was purified using SPRI beads.

    SYBR Green Assay:

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: Following sample harvesting, purification and dilution 1:100, a two-step second PCR of 17 cycles (5 cycles with annealing temperature of 55°C + 12 cycles with annealing temperature of 70°C) is performed to generate a dual indexed sequencing library (note that the ‘Outer primers’ sequences were as described for the second PCR primer sequences in ( ). .. The first PCR (in the AA chip) is done using the manufacturer recommended enzyme: FastStart High Fidelity PCR System, dNTPack (Roche) while the second PCR is done using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the addition of SYBR green I (LONZA) at a final concentration of X1, to enable real time tracking of amplification. .. Following second PCR, each sample was purified using SPRI beads.

    Concentration Assay:

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: Following sample harvesting, purification and dilution 1:100, a two-step second PCR of 17 cycles (5 cycles with annealing temperature of 55°C + 12 cycles with annealing temperature of 70°C) is performed to generate a dual indexed sequencing library (note that the ‘Outer primers’ sequences were as described for the second PCR primer sequences in ( ). .. The first PCR (in the AA chip) is done using the manufacturer recommended enzyme: FastStart High Fidelity PCR System, dNTPack (Roche) while the second PCR is done using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the addition of SYBR green I (LONZA) at a final concentration of X1, to enable real time tracking of amplification. .. Following second PCR, each sample was purified using SPRI beads.

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  • 86
    Roche expand long range dntpack kit
    Expand Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long range dntpack kit/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack kit - by Bioz Stars, 2021-07
    86/100 stars
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    Roche expand long range dntpack
    Expand Long Range Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long range dntpack/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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    Roche expand longrange dntpack system
    Expand Longrange Dntpack System, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand longrange dntpack system/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand longrange dntpack system - by Bioz Stars, 2021-07
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      Buy from Supplier

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