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Roche dntpack
Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntpack/product/Roche
Average 90 stars, based on 72 article reviews
Price from $9.99 to $1999.99
dntpack - by Bioz Stars, 2020-07
90/100 stars

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Related Articles

SYBR Green Assay:

Article Title: Transcriptional Reprogramming of Arabidopsis thaliana Defence Pathways by the Entomopathogen Beauveria bassiana Correlates With Resistance Against a Fungal Pathogen but Not Against Insects
Article Snippet: .. A total of 2 μg of DNase-treated RNA was then reverse-transcribed into the first-strand cDNA using SuperScript® III First-Strand Synthesis System (Invitrogen). cDNA synthesis was performed according to the manufacturer’s instructions, using Oligo dB 12-18 primer and including an RNase H digestion as a last step to remove RNA template from the cDNA:RNA hybrid molecule. qPCR was performed in triplicate from three biological replicates with a reaction mixture containing gene specific primers, cDNA template with a dilution value of 1:10, SYBR Green reagent, ROX Reference Dye to normalise the fluorescent reporter signal and the FastStartTM Taq DNA Polymerase, dNTPack (Roche). .. The thermal cycling conditions were 95°C for 10 min followed by 95°C for 15′′ , 60°C for 45′′ and 72°C for 45′′ for 40 cycles, followed by melting curve step at 95°C for 15′′ , 60°C for 1′ and 95°C for 15′′ to validate amplicon specificity.

Amplification:

Article Title: Establishing RNAi in a Non-Model Organism: The Antarctic Nematode Panagrolaimus sp. DAW1
Article Snippet: .. Target genes were PCR amplified from cDNA with gene specific primers using the Taq DNA Polymerase, dNTPack (Roche, Basel, Switzerland) (Table A in ). .. PCR products were TA-cloned into the double IPTG inducible T7 RNA polymerase promoter vector pLitmus28i and their identity confirmed by sequencing.

Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
Article Snippet: .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

Article Title: X-linked intellectual disability type Nascimento is a clinically distinct, probably underdiagnosed entity
Article Snippet: .. UBE2A exons were amplified using the FastStart Taq DNA Polymerase, dNTPack (Roche) and purified with AmpureXP (Beckman Coulter, Inc) following standard protocols. .. BigDye Terminator v3.1 Cycle Sequencing Kit was used for sequencing reactions prior to sequencing on a 48-capillary 3730 DNA Analyzer (Applied Biosystems).

Agarose Gel Electrophoresis:

Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
Article Snippet: .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

Purification:

Article Title: X-linked intellectual disability type Nascimento is a clinically distinct, probably underdiagnosed entity
Article Snippet: .. UBE2A exons were amplified using the FastStart Taq DNA Polymerase, dNTPack (Roche) and purified with AmpureXP (Beckman Coulter, Inc) following standard protocols. .. BigDye Terminator v3.1 Cycle Sequencing Kit was used for sequencing reactions prior to sequencing on a 48-capillary 3730 DNA Analyzer (Applied Biosystems).

Electrophoresis:

Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
Article Snippet: .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

Polymerase Chain Reaction:

Article Title: Read count-based method for high-throughput allelic genotyping of transposable elements and structural variants
Article Snippet: .. We used the FastStart High Fidelity PCR System, dNTPack (Roche), which was also used in the Access Array, to assemble the reactions. ..

Article Title: Establishing RNAi in a Non-Model Organism: The Antarctic Nematode Panagrolaimus sp. DAW1
Article Snippet: .. Target genes were PCR amplified from cDNA with gene specific primers using the Taq DNA Polymerase, dNTPack (Roche, Basel, Switzerland) (Table A in ). .. PCR products were TA-cloned into the double IPTG inducible T7 RNA polymerase promoter vector pLitmus28i and their identity confirmed by sequencing.

Article Title: High-Resolution Hepatitis C Virus Subtyping Using NS5B Deep Sequencing and Phylogeny, an Alternative to Current Methods
Article Snippet: .. Heminested PCR was performed using a FastStart High Fidelity PCR System, dNTPack (Roche Applied Science, Basel, Switzerland), 5 μl from the PCR, and a pair of upstream (13N5Bo8254) and downstream (13N5Bo8641) primers ( ). ..

Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
Article Snippet: .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

Article Title: A genome-wide association study reveals a locus for bilateral iridal hypopigmentation in Holstein Friesian cattle
Article Snippet: .. PCR was performed in a total volume of 25 μl using FastStart Taq DNA Polymerase, dNTPack (Roche Diagnostics, Mannheim, Germany). .. One reaction mix (25 μl) included 1.5 U Faststart Taq DNA Polymerase, 200 μmol/L dNTP, 0.4 μmol/L of each primer, 1× PCR reaction buffer (including 20 mM MgCl2 ), 1× Q-Solution (Qiagen, Hilden, Germany) and 40 ng of DNA.

Article Title: Reduced Human Platelet Uptake by Pig Livers Deficient in the Asialoglycoprotein Receptor-1 Protein
Article Snippet: .. Pwo SuperYield DNA Polymerase, dNTPack (Roche, Indianapolis, IN) was used and PCR conditions were as follows: 94°C, 2 minutes; 94°C, 15 seconds, 60°C, 30 seconds and 72°C, 50 seconds for 15 cycles; 94°C, 15 seconds, 60°C, 30 seconds and 72°C, 50 seconds with 5 seconds added to each elongation for 25 cycles; and a final extension step of 72°C for 5 minutes. ..

Nested PCR:

Article Title: Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
Article Snippet: .. This nested PCR used the FastStart High Fidelity PCR system, dNTPack (Roche Applied Science), with a previously described protocol.56 Amplification products were analyzed by electrophoresis on 2% agarose gel. ..

Real-time Polymerase Chain Reaction:

Article Title: Transcriptional Reprogramming of Arabidopsis thaliana Defence Pathways by the Entomopathogen Beauveria bassiana Correlates With Resistance Against a Fungal Pathogen but Not Against Insects
Article Snippet: .. A total of 2 μg of DNase-treated RNA was then reverse-transcribed into the first-strand cDNA using SuperScript® III First-Strand Synthesis System (Invitrogen). cDNA synthesis was performed according to the manufacturer’s instructions, using Oligo dB 12-18 primer and including an RNase H digestion as a last step to remove RNA template from the cDNA:RNA hybrid molecule. qPCR was performed in triplicate from three biological replicates with a reaction mixture containing gene specific primers, cDNA template with a dilution value of 1:10, SYBR Green reagent, ROX Reference Dye to normalise the fluorescent reporter signal and the FastStartTM Taq DNA Polymerase, dNTPack (Roche). .. The thermal cycling conditions were 95°C for 10 min followed by 95°C for 15′′ , 60°C for 45′′ and 72°C for 45′′ for 40 cycles, followed by melting curve step at 95°C for 15′′ , 60°C for 1′ and 95°C for 15′′ to validate amplicon specificity.

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  • 90
    Roche dntpack
    Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntpack/product/Roche
    Average 90 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    dntpack - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    85
    Roche long range dntpack
    Long Range Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range dntpack/product/Roche
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    long range dntpack - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    89
    Roche expand long range dntpack kit
    Expand Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long range dntpack kit/product/Roche
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack kit - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

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