Structured Review

TaKaRa dntp
Dntp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntp/product/TaKaRa
Average 97 stars, based on 72 article reviews
Price from $9.99 to $1999.99
dntp - by Bioz Stars, 2020-01
97/100 stars

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Amplification:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: Briefly, two portions of the HFE gene surrounding both mutations were amplified separately using PCR with HFE -specific primers. .. The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China).

Agarose Gel Electrophoresis:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China). .. The products were resolved on 2% agarose gel and stained by ethidium bromide (EB).

Article Title: MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis
Article Snippet: The 25 µl PCR reaction system contained 0.5 µl 10 µM forward primer, 0.5 µl 10 µM reverse primer, 2 µl 2.5 mM dNTP (cat. no. D40300A; Takara), 0.25 µl Ex Taq (cat. no. DRR100A; Takara), 2.5 µl 10 fold Ex Taq E buffer, 1 µl cDNA and ddH2 0. .. The PCR was conducted on an ABI7900 thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc.) and products were detected on a 1% agarose gel with 0.5 µg/ml ethidium bromide.

Synthesized:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: These primers was designed using Primer Express v5.0 software (Applied Biosystem) based on the published sequence of the HFE gene and synthesized from Sangon Biotech Co., Ltd (Shanghai, China). .. The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China).

Isolation:

Article Title: MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis
Article Snippet: EPCs were isolated from rat bone marrow cells, according to a standard procedure ( ) and then von Willebrand factor (vWF; forward primer, 5′-GCTCCAGCAAGTTGAAGACC-3′, reverse primer, 5′-GCAAGTCACTGTGTGGCACT-3′), kinase insert domain receptor (KDR; forward primer, 5′-CTTTTGGTAGCGGGATGAAA-3′, reverse primer, 5′-TCCATTGAAATGGGATTGGT-3′), TEK receptor tyrosine kinase (Tek; forward primer, 5′-ACAATCGTGGACGGCTATTC-3′, reverse primer, 5′-AAGGTCTTTAGGGGCTGGAA-3′), CD34 (forward 5′-ACTTCTGTTGCCTCGGAGAA-3′; reverse 5′-ACGGTTGGGTAAGTCTGTGG-3′), CD133 (forward 5′-CCTCTGGTGGGGTATTTCTT-3′; reverse 5′-AGGTGCTGTTCATGTTCTCC-3′), and nitric oxide synthase 3 (eNOS; forward primer, 5′-CACAGGCATCACCAGGAAGAA-3′, reverse primer, 5′-ATACCAGTGGGTCCGAGCA-3′) gene expression were determined by semi-quantitative PCR to confirm the success of EPC isolation ( , ). β-actin (F240; forward primer, 5′-CACGATGGAGGGGCCGGACTCATC-3′ and R240; reverse primer, 5′-TAAAGACCTCTATGCCAACACAGT-3′) was used as the reference gene. .. The 25 µl PCR reaction system contained 0.5 µl 10 µM forward primer, 0.5 µl 10 µM reverse primer, 2 µl 2.5 mM dNTP (cat. no. D40300A; Takara), 0.25 µl Ex Taq (cat. no. DRR100A; Takara), 2.5 µl 10 fold Ex Taq E buffer, 1 µl cDNA and ddH2 0.

Size-exclusion Chromatography:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China). .. The PCR conditions were 3 min at 94°C, followed by 35 cycles of 30 sec at 94°C, 30 sec at 56°C and 1 min at 72°C, and finally, 5 min at 72°C, performed using the autorisierter thermocycler (eppendorf Mastercycler personal, Germany).

Electrophoresis:

Article Title: MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis
Article Snippet: The 25 µl PCR reaction system contained 0.5 µl 10 µM forward primer, 0.5 µl 10 µM reverse primer, 2 µl 2.5 mM dNTP (cat. no. D40300A; Takara), 0.25 µl Ex Taq (cat. no. DRR100A; Takara), 2.5 µl 10 fold Ex Taq E buffer, 1 µl cDNA and ddH2 0. .. Gels were observed with JY02S Gel Imager (Beijing Junyi Electrophoresis Co., Ltd.) and the acquired gel images were processed by Photoshop CS5 (Adobe Systems, Inc.).

CTG Assay:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: .. The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China). .. The PCR conditions were 3 min at 94°C, followed by 35 cycles of 30 sec at 94°C, 30 sec at 56°C and 1 min at 72°C, and finally, 5 min at 72°C, performed using the autorisierter thermocycler (eppendorf Mastercycler personal, Germany).

Incubation:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China). .. After the PCR amplification, restriction digests were performed directly with 10 µl of the PCR mixtures by the addition of 5 U SnaBI for C282Y (BioLabs, New England, MA, USA) or BclI (BioLabs) for H63D and the corresponding buffers (2 µl 10×NEB4 Buffer added to 2 µl 10×BSA, 5 µl sterile water and 2 µl 10×NEB3 Buffer and adding sterile water to reach 20 µl) and incubated for 2 h at 37°C for the SnaB I restriction or overnight at 50°C for the Bcl I restriction.

Polymerase Chain Reaction:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: .. The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China). .. The PCR conditions were 3 min at 94°C, followed by 35 cycles of 30 sec at 94°C, 30 sec at 56°C and 1 min at 72°C, and finally, 5 min at 72°C, performed using the autorisierter thermocycler (eppendorf Mastercycler personal, Germany).

Article Title: MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis
Article Snippet: .. The 25 µl PCR reaction system contained 0.5 µl 10 µM forward primer, 0.5 µl 10 µM reverse primer, 2 µl 2.5 mM dNTP (cat. no. D40300A; Takara), 0.25 µl Ex Taq (cat. no. DRR100A; Takara), 2.5 µl 10 fold Ex Taq E buffer, 1 µl cDNA and ddH2 0. ..

Expressing:

Article Title: MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis
Article Snippet: EPCs were isolated from rat bone marrow cells, according to a standard procedure ( ) and then von Willebrand factor (vWF; forward primer, 5′-GCTCCAGCAAGTTGAAGACC-3′, reverse primer, 5′-GCAAGTCACTGTGTGGCACT-3′), kinase insert domain receptor (KDR; forward primer, 5′-CTTTTGGTAGCGGGATGAAA-3′, reverse primer, 5′-TCCATTGAAATGGGATTGGT-3′), TEK receptor tyrosine kinase (Tek; forward primer, 5′-ACAATCGTGGACGGCTATTC-3′, reverse primer, 5′-AAGGTCTTTAGGGGCTGGAA-3′), CD34 (forward 5′-ACTTCTGTTGCCTCGGAGAA-3′; reverse 5′-ACGGTTGGGTAAGTCTGTGG-3′), CD133 (forward 5′-CCTCTGGTGGGGTATTTCTT-3′; reverse 5′-AGGTGCTGTTCATGTTCTCC-3′), and nitric oxide synthase 3 (eNOS; forward primer, 5′-CACAGGCATCACCAGGAAGAA-3′, reverse primer, 5′-ATACCAGTGGGTCCGAGCA-3′) gene expression were determined by semi-quantitative PCR to confirm the success of EPC isolation ( , ). β-actin (F240; forward primer, 5′-CACGATGGAGGGGCCGGACTCATC-3′ and R240; reverse primer, 5′-TAAAGACCTCTATGCCAACACAGT-3′) was used as the reference gene. .. The 25 µl PCR reaction system contained 0.5 µl 10 µM forward primer, 0.5 µl 10 µM reverse primer, 2 µl 2.5 mM dNTP (cat. no. D40300A; Takara), 0.25 µl Ex Taq (cat. no. DRR100A; Takara), 2.5 µl 10 fold Ex Taq E buffer, 1 µl cDNA and ddH2 0.

Sequencing:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: These primers was designed using Primer Express v5.0 software (Applied Biosystem) based on the published sequence of the HFE gene and synthesized from Sangon Biotech Co., Ltd (Shanghai, China). .. The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China).

Staining:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China). .. The products were resolved on 2% agarose gel and stained by ethidium bromide (EB).

Variant Assay:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: .. The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China). .. The PCR conditions were 3 min at 94°C, followed by 35 cycles of 30 sec at 94°C, 30 sec at 56°C and 1 min at 72°C, and finally, 5 min at 72°C, performed using the autorisierter thermocycler (eppendorf Mastercycler personal, Germany).

Derivative Assay:

Article Title: MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis
Article Snippet: EPC adhesion and proliferation The EPCs isolated and used in the current study were derived from the rat bone marrow where no vascular ECs are present. .. The 25 µl PCR reaction system contained 0.5 µl 10 µM forward primer, 0.5 µl 10 µM reverse primer, 2 µl 2.5 mM dNTP (cat. no. D40300A; Takara), 0.25 µl Ex Taq (cat. no. DRR100A; Takara), 2.5 µl 10 fold Ex Taq E buffer, 1 µl cDNA and ddH2 0.

Software:

Article Title: The Effect of the Hemochromatosis (HFE) Genotype on Lead Load and Iron Metabolism among Lead Smelter Workers
Article Snippet: These primers was designed using Primer Express v5.0 software (Applied Biosystem) based on the published sequence of the HFE gene and synthesized from Sangon Biotech Co., Ltd (Shanghai, China). .. The PCR reactions consisted of 20 µM of each primer, forward PCR primer, 5′- TGT GGA GCC TCA ACA TCCT-3′ ; reverse PCR primer, 5′- TGA AAA GCT CTG ACA ACC TCA-3′ for the H63D variant (rs1799945), and forward PCR primer, 5′ -TCC AGT CTT CCT GGC AA-3′; reverse PCR primer, 5′-TTC TAG CTC CTG GCT CTCA-3′ for the C282Y variant (rs1800562), 2.5 µl 10×Reaction Buffer (containing Mg2+ ), 2 µl dNTP (2.5 mM), 0.125 µl Ex Taq DNA polymerase(5 U/µl), and 4 µl genomic DNA; then sterile water was added to 25 µl of Ex Taq DNA polymerase, 10×Reaction Buffer, dNTP (100 mM) and 6×Loading Buffer, which were purchased from Takara (Dalian, China).

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    TaKaRa dntp mixture
    Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 67 article reviews
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    TaKaRa takara dntp mixture
    Takara Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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